pycom15g07770

VID27 cytoplasmic protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009824
Physical Location & Seq
Forward (+)
4811145 .. 4815184
4040 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3408 bp
ATGAACTTGGCCTTTTCTTTCGGTCTCTCTGCGAAGCCCAACCTTCCCTATTTCCACTCTCTCCTAAAATCTCACAGCGAAAGCAAAAATCCTAAACAGGCACTGCTTCTTTTTCGCCAGTTGTTTGAGTACAATTTGAAGCCCAATGATCTCACTTTCTCTCTGCTCCTTAAATCCTGCACTTCTTCCTCCTCCTCGGGTTTTAATTCAGTGAGCTCAAAACTAGAAGCAAATCAGATTCATACCCACTTGCTGAAATCAGGTGTCAATCGATATGTATATGTGAGTACTGCTCTTCTTGATTTGTATATGAAATTGGATTGTGTAAGGGCTGCCCAGAAGGTGTTTGATGATATGCCTGACAGAGATGTTGTCTCATGGAATGCGTTGATTTCTGGGTTTTCTCGAAATGGGTATGCTTTTGAAGCGTTGAATTGCTTTGTCCGAATGTGTAGAGAGGGGCTTTGTCCTTGTCGGACGACTTTGGTTAGTTTGGTTCCGTCGTGTGGTAGGCAAGAGTTAGTTTTTCTTGGGAAATGTGTTCATGGGTTTGGAATTAAGGCGGGCCTGGATTCGGATTCTAACGTGAAGAATGTGCTTACCTCGATGTATGCTAAATCTGCAGATTTGGAGGCGGCGGAGCTCTTGTTTGAAGAGATAGTTGACAAGAGTGTAGTTTCTTGGAATACTATGATTGGCGCTTATGGACAAAATGGTTTCTTTCATGAGGCAATGCTTGTCTTTAAACAAATGCAGGAGGAAAATGTTCCGGCCAATCTGGTGACCATGGTAAGCCTCCTGTCAGCAAATGCAAATCCTGAGTCTACCCATTGTCATGCCCTTAAAACTGGTCATGCAAATGATGCTTCTGTGATTACTTCGCTAATTTGTGAATATGCAACACGAGGGAACACAGAATCCGGAAAACTGCTTTACAGGTCATTGCCTCAGAAGAACTTGGTTTCCCTGACTGCAATTATTTCAAGCTATGCTGAGAAAGATAACATAGCTCAGGTACTGGAATGCTTTGCTCACTTGCAGCGGTTAGACATGAAACTGGACGCAGTTTCTATGGTTTGCATCCTTCATGGGATAAAAGTCCCTGTTGATATTGGCCTTGGACTTGCTTTCCATGGTTATGGAATAAAGATTGGGCTGACGGCTGATTCTTTGGTTGCAAATTGTTTGATAAGCATGTACTCGAGGTTTGACAAGATAGAAGCCGCTTTTTCTTTATTCCTTGATATGCAAGCAAAATCGTTGCTTACTTGGAATTCTTTGATATCGGGATGTGTACAAGTGGGAAGGTCAAGTGATGCCCTGGACTTGTTTCTCCAAATGAAAATGTCCGGATATAGTCCAGATAGCATCACTGTTTCTAGCTTACTATCTGGGTGTGGCCAAATTGGGTACTTGCGATTTGGGGAGAAACTTCATAACTATGTCCTCCGAAACAATCTTGAAGTGGAAGATTTTGTTGGAACCGCTCTTATAGACATGTACACCAAGTGTGGAAGAATAGAGCTTGCTGAAAGGGTGTTTAAGAGCATCCAAGAACCATGTTTGGCATCATGGAACTCAATCCTCTTGGGTTATAGCCACTACGGGTCAGAACATAGAGCTCTTGCTTGCTTCTTGAAAATGCAAGAGCAGGGGATAATGCCCGATAATATCACTTTTTTGGGAGTTTTGGTTGCTTGTGCTCATGGAGGTTTTGTTGAGGAGGGAAGAAAATATTTCCAAATAATGAGGGAAGAGTTCAATATAGTGCCATGCTTGCAACACTGCGCTTGCATGGTTGGTCTCTTTGGCCGCGCGGGCTTCTTCCAGGAGGCATTGGTTTTCATCAGAGATATGGAGATGGAGCCCGACTTCGCAGTAAACCCTAACGGAAAAGTAAGACGATGCCAAAAAACCGCAAAAGCGCTCCCAAATTTGAAATTTGAACTTTTCATCCCAACACACCATCTCACTCTCAGACTCCCAAAGCTCTCATCTTTCACATACCATTTCCCAAATCTTCGTACAAAAATGGGCGGAGCTCACAGCCGCGAACACCTCGAGCTTTCCGACTCCGACGAGGAAGAGAGCGAAGAGCAGGAGAGCTACGAGGACGTGGAGGAAACTGAAGCCGACCAGCGCCAGAGATCCGCCGAGCGGGGAACCAAAACGCCGTCGTCGGCTGCATTAGACGACGTGGACGCCAAGCTCAAGGCTTTGAGTCTCAAGTACGGGTCTTCTTCCAGGAACACGAATCTGAAGAATCCGGTGAAGCTGTACCTCCACATTGGAGGGAACACCCCAAAAGCGAAATGGGTAACTTCCGAAAAACTCACTTCTTTCAATTTTATTAAGACATCTCGAATGGAGGTTGATAATGATGAGGAGGAAGAGGAAGAAGAGGAATTTGATGAGGAGGGTTTGTGGGTTTTGAGGGTGGGGGCCAAAGTTAAGGCTAAGGTATCGGCCGAGATGCAATTGCTCACTTTTGCCGATCAGCGGCGTGTCGATTTCTTTGCCAGAGGCGTGTGGGCAATGAAGTTTTTCAGTGATGAGGAGTATAGTGCTTTTGTGATTAAGTACAATGACTGTTTGTTTGAGAACACTTATGGGTATGAGGCCACTGAGGAGAACAGGGTGAAGGTTTATGGGAAAGATTTTGTTGGGTGGGCGAAGCCCGAAATGGCGGATGATTCGGTCTGGGAAGATGCGGAGGATAATTTTTTGAAGAGCCCTGGCTCAGCAACACCAGTGAGGGGAAATCAGAATTTGAGAGAGGAGTTTGAGGAGGCCCACAATGGCGGTATACAGAGCTTGGCATTAGGTGCATTGGACAATAGTTTCTTGGTGGGGAACTCTGGCATTCAGGTTGTTAAGAATTTTGCTCATGGAATACATGGTAAAGGTGTTTATGTGAATTTTGATGATGGGAGCTATGGAGGCGGTTCAAATTTGGCACGGTCTACACCTAGGAAGGCTCTGCTGATGAAGGCCGAGACTAACATGCTCCTTATGAGTCCGATGAATGAAGGGAAGCCTCATACAACCGGGCTCCATCAGCTTGATATCGAAACCGGGAAGGTTGTTACTGAGTGGAAGTTTGAGAAAGATGGAGCTGACATTACAATGAGGGATATCACAAATGATAGTAAGGGAGCACAGTTGGACCCCTCAGGATCGACATTCTTGGGTTTGGATGACAACAGGCTTTGCCGGTGGGATATGCGTGATAGGAAGGGAATGGTTCAGGACCTTGCAGCTTCTAGTGGTCCTGTTCTCAATTGGAACCAAGGGCATCAGTTCTCAAGAGGGACTAACTTTCAGTGCTTTGCTAGCACTGGTGATGGCTCAATTGCTGTTGGGTCGCTTGATGGGAAGATCAGGTTGTACGATCGATCAATTCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 VID27

Protein Analysis

1136

Amino Acids

126.35

Weight (kDa)

5.72

Isoelectric Point (pI)

34.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR PF01535 125 - 155 7.2e-07 PPR repeat
PPR_2 PF13041 224 - 269 1.4e-09 PPR repeat family
PPR PF01535 226 - 256 6.4e-09 PPR repeat
PPR_2 PF13041 422 - 466 1.4e-11 PPR repeat family
PPR PF01535 423 - 451 4.3e-07 PPR repeat
DUF7135 PF23581 719 - 884 1.6e-74 Domain of unknown function (DUF7135)
VID27 PF08553 988 - 1132 2.4e-16 VID27 C-terminal WD40-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 1487, 2158
AccI GTMKAC 3 cut(s) 824, 2806, 2963
AccII CGCG 3 cut(s) 1815, 1817, 2052
AccIII TCCGGA 2 cut(s) 920, 1349
AclWI GGATC 2 cut(s) 2142, 3184
AcoI YGGCCR 4 cut(s) 771, 1399, 1810, 2466
AcsI RAATTY 8 cut(s) 1273, 1933, 1940, 2405, 2767, 2878, 2917, 2950
AcuI CTGAAG 2 cut(s) 2148, 2279
AcyI GRCGYC 1 cut(s) 2202
AdeI CACNNNGTG 1 cut(s) 1509
AfeI AGCGCT 1 cut(s) 1926
AfiI CCNNNNNNNGG 5 cut(s) 506, 574, 2157, 2499, 2756
AflIII ACRYGT 1 cut(s) 1497
AjiI CACGTC 2 cut(s) 2116, 2197
AjnI CCWGG 5 cut(s) 567, 1320, 1827, 2243, 2734
AjuI GAANNNNNNNTTGG 2 cut(s) 1461, 1493
AloI GAACNNNNNNTCC 6 cut(s) 902, 934, 947, 979, 1938, 1970
Alw21I GWGCWC 6 cut(s) 218, 645, 1624, 1705, 2044, 3160
Alw26I GTCTC 5 cut(s) 29, 379, 1808, 2228, 2990
AlwI GGATC 2 cut(s) 2142, 3184
AlwNI CAGNNNCTG 2 cut(s) 103, 1018
Ama87I CYCGRG 3 cut(s) 196, 1201, 2060
Aor13HI TCCGGA 2 cut(s) 920, 1349
Aor51HI AGCGCT 1 cut(s) 1926
ApeKI GCWGC 4 cut(s) 332, 1039, 2183, 3257
ApoI RAATTY 8 cut(s) 1273, 1933, 1940, 2405, 2767, 2878, 2917, 2950
ArsI GACNNNNNNTTYG 4 cut(s) 806, 838, 2579, 2611
Asp700I GAANNNNTTC 2 cut(s) 765, 2543
AspA2I CCTAGG 1 cut(s) 2969
AspLEI GCGC 5 cut(s) 701, 1790, 1817, 1927, 2142
AspS9I GGNCC 6 cut(s) 565, 2442, 2791, 3166, 3250, 3269
AsuC2I CCSGG 2 cut(s) 3049, 3076
AsuHPI GGTGA 4 cut(s) 793, 2281, 2650, 3353
AsuNHI GCTAGC 1 cut(s) 3332
AvaI CYCGRG 3 cut(s) 196, 1201, 2060
AvaII GGWCC 3 cut(s) 3166, 3250, 3269
AvrII CCTAGG 1 cut(s) 2969
AxyI CCTNAGG 1 cut(s) 3172
BalI TGGCCA 1 cut(s) 1401
BanII GRGCYC 7 cut(s) 218, 645, 1624, 1869, 2044, 2735, 3054
BauI CACGAG 1 cut(s) 903
BbsI GAAGAC 1 cut(s) 2229
Bbv12I GWGCWC 6 cut(s) 218, 645, 1624, 1705, 2044, 3160
BbvI GCAGC 4 cut(s) 319, 1051, 2170, 3269
BccI CCATC 7 cut(s) 1855, 1974, 2921, 3063, 3104, 3338, 3365
BceAI ACGGC 2 cut(s) 1176, 2158
BcgI CGANNNNNNTGC 2 cut(s) 2498, 2532
BciT130I CCWGG 5 cut(s) 569, 1322, 1829, 2245, 2736
BcnI CCSGG 2 cut(s) 3049, 3076
BcoDI GTCTC 5 cut(s) 29, 379, 1808, 2228, 2990
BfaI CTAG 5 cut(s) 224, 1380, 2970, 3264, 3333
BfmI CTRYAG 1 cut(s) 621
BfoI RGCGCY 3 cut(s) 702, 1928, 2143
BglI GCCNNNNNGGC 1 cut(s) 1818
BisI GCNGC 9 cut(s) 333, 636, 1040, 1224, 1813, 2050, 2184, 2501, 3258
BlnI CCTAGG 1 cut(s) 2969
BlpI GCTNAGC 1 cut(s) 2740
BlsI GCNGC 9 cut(s) 334, 637, 1041, 1225, 1814, 2051, 2185, 2502, 3259
BmcAI AGTACT 1 cut(s) 289
Bme1390I CCNGG 7 cut(s) 569, 1322, 1829, 2245, 2736, 3049, 3076
Bme18I GGWCC 3 cut(s) 3166, 3250, 3269
BmeT110I CYCGRG 3 cut(s) 196, 1201, 2060
BmgBI CACGTC 2 cut(s) 2116, 2197
BmgT120I GGNCC 6 cut(s) 565, 2442, 2791, 3166, 3250, 3269
BmiI GGNNCC 8 cut(s) 498, 1483, 1866, 2164, 2443, 3053, 3168, 3287
BmrFI CCNGG 7 cut(s) 569, 1322, 1829, 2245, 2736, 3049, 3076
BmtI GCTAGC 1 cut(s) 3336
BpiI GAAGAC 1 cut(s) 2229
BplI GAGNNNNNCTC 2 cut(s) 277, 309
Bpu10I CCTNAGC 2 cut(s) 1011, 2457
Bpu1102I GCTNAGC 1 cut(s) 2740
BpuEI CTTGAG 3 cut(s) 2195, 2210, 3289
BpuMI CCSGG 2 cut(s) 3049, 3076
Bsa29I ATCGAT 2 cut(s) 271, 3394
BsaHI GRCGYC 1 cut(s) 2202
BsaI GGTCTC 2 cut(s) 29, 1808
BsaJI CCNNGG 8 cut(s) 195, 786, 1117, 1132, 1320, 2734, 2969, 3289
BsaWI WCCGGW 3 cut(s) 920, 1349, 2266
BsaXI ACNNNNNCTCC 8 cut(s) 632, 662, 1677, 1701, 1707, 1731, 2408, 2438
Bsc4I CCNNNNNNNGG 5 cut(s) 506, 574, 2157, 2499, 2756
Bse118I RCCGGY 1 cut(s) 3213
Bse1I ACTGG 6 cut(s) 118, 853, 1023, 1062, 2750, 3343
Bse21I CCTNAGG 1 cut(s) 3172
Bse3DI GCAATG 3 cut(s) 738, 941, 2541
BseAI TCCGGA 2 cut(s) 920, 1349
BseBI CCWGG 5 cut(s) 569, 1322, 1829, 2245, 2736
BseCI ATCGAT 2 cut(s) 271, 3394
BseDI CCNNGG 8 cut(s) 195, 786, 1117, 1132, 1320, 2734, 2969, 3289
BseGI GGATG 6 cut(s) 1080, 1295, 1548, 1953, 2695, 3202
BseLI CCNNNNNNNGG 5 cut(s) 506, 574, 2157, 2499, 2756
BseMI GCAATG 3 cut(s) 738, 941, 2541
BseMII CTCAG 9 cut(s) 810, 962, 984, 1025, 1990, 2616, 2754, 3081, 3186
BseNI ACTGG 6 cut(s) 118, 853, 1023, 1062, 2750, 3343
BseRI GAGGAG 9 cut(s) 181, 184, 1736, 2399, 2429, 2570, 2642, 2792, 2801
BseX3I CGGCCG 1 cut(s) 2466
BseXI GCAGC 4 cut(s) 319, 1051, 2170, 3269
BsgI GTGCAG 1 cut(s) 163
Bsh1236I CGCG 3 cut(s) 1815, 1817, 2052
Bsh1285I CGRYCG 2 cut(s) 2469, 3394
BshVI ATCGAT 2 cut(s) 271, 3394
BsiEI CGRYCG 2 cut(s) 2469, 3394
BsiHKAI GWGCWC 6 cut(s) 218, 645, 1624, 1705, 2044, 3160
BsiHKCI CYCGRG 3 cut(s) 196, 1201, 2060
BsiSI CCGG 7 cut(s) 770, 921, 1350, 2267, 3048, 3075, 3214
BslFI GGGAC 2 cut(s) 1085, 3325
BslI CCNNNNNNNGG 5 cut(s) 506, 574, 2157, 2499, 2756
BsmAI GTCTC 5 cut(s) 29, 379, 1808, 2228, 2990
BsmFI GGGAC 2 cut(s) 1085, 3325
BsmI GAATGC 3 cut(s) 388, 1028, 2862
Bso31I GGTCTC 2 cut(s) 29, 1808
BsoBI CYCGRG 3 cut(s) 196, 1201, 2060
Bsp1286I GDGCHC 9 cut(s) 218, 645, 1624, 1705, 1869, 2044, 2735, 3054, 3160
Bsp13I TCCGGA 2 cut(s) 920, 1349
Bsp1407I TGTACA 2 cut(s) 1294, 1500
Bsp143I GATC 7 cut(s) 148, 2147, 2494, 3176, 3378, 3391, 3395
Bsp1720I GCTNAGC 1 cut(s) 2740
Bsp19I CCATGG 2 cut(s) 786, 1132
BspCNI CTCAG 9 cut(s) 811, 961, 985, 1024, 1989, 2617, 2753, 3082, 3185
BspDI ATCGAT 2 cut(s) 271, 3394
BspEI TCCGGA 2 cut(s) 920, 1349
BspFNI CGCG 3 cut(s) 1815, 1817, 2052
BspHI TCATGA 1 cut(s) 724
BspLI GGNNCC 8 cut(s) 498, 1483, 1866, 2164, 2443, 3053, 3168, 3287
BspMAI CTGCAG 1 cut(s) 625
BspOI GCTAGC 1 cut(s) 3336
BspPI GGATC 2 cut(s) 2142, 3184
BspQI GCTCTTC 3 cut(s) 300, 2088, 2723
BspTNI GGTCTC 2 cut(s) 29, 1808
BsrBI CCGCTC 2 cut(s) 1487, 2158
BsrDI GCAATG 3 cut(s) 738, 941, 2541
BsrFI RCCGGY 1 cut(s) 3213
BsrGI TGTACA 2 cut(s) 1294, 1500
BsrI ACTGG 6 cut(s) 118, 853, 1023, 1062, 2750, 3343
BssAI RCCGGY 1 cut(s) 3213
BssECI CCNNGG 8 cut(s) 195, 786, 1117, 1132, 1320, 2734, 2969, 3289
BssMI GATC 7 cut(s) 148, 2147, 2494, 3176, 3378, 3391, 3395
BssNAI GTATAC 1 cut(s) 2807
BssNI GRCGYC 1 cut(s) 2202
BssSI CACGAG 1 cut(s) 903
BssT1I CCWWGG 5 cut(s) 786, 1117, 1132, 2969, 3289
Bst1107I GTATAC 1 cut(s) 2807
Bst2BI CACGAG 1 cut(s) 903
Bst2UI CCWGG 5 cut(s) 569, 1322, 1829, 2245, 2736
Bst4CI ACNGT 4 cut(s) 1375, 2591, 2961, 3162
Bst6I CTCTTC 8 cut(s) 300, 648, 1749, 2079, 2088, 2385, 2394, 2723
BstACI GRCGYC 1 cut(s) 2202
BstAPI GCANNNNNTGC 2 cut(s) 863, 2825
BstAUI TGTACA 2 cut(s) 1294, 1500
BstC8I GCNNGC 8 cut(s) 565, 1251, 1527, 1630, 1778, 1792, 1819, 3334
BstDSI CCRYGG 2 cut(s) 786, 1132
BstEII GGTNACC 1 cut(s) 781
BstF5I GGATG 6 cut(s) 1080, 1295, 1548, 1953, 2695, 3202
BstFNI CGCG 3 cut(s) 1815, 1817, 2052
BstH2I RGCGCY 3 cut(s) 702, 1928, 2143
BstHHI GCGC 5 cut(s) 701, 1790, 1817, 1927, 2142
BstKTI GATC 7 cut(s) 151, 2150, 2497, 3179, 3381, 3394, 3398
BstMAI GTCTC 5 cut(s) 29, 379, 1808, 2228, 2990
BstMBI GATC 7 cut(s) 148, 2147, 2494, 3176, 3378, 3391, 3395
BstMCI CGRYCG 2 cut(s) 2469, 3394
BstMWI GCNNNNNNNGC 9 cut(s) 425, 620, 863, 1777, 1818, 2825, 2940, 3058, 3333
BstNI CCWGG 5 cut(s) 569, 1322, 1829, 2245, 2736
BstNSI RCATGY 3 cut(s) 1198, 1501, 3007
BstPI GGTNACC 1 cut(s) 781
BstSCI CCNGG 7 cut(s) 567, 1320, 1827, 2243, 2734, 3047, 3074
BstSFI CTRYAG 1 cut(s) 621
BstUI CGCG 3 cut(s) 1815, 1817, 2052
BstV1I GCAGC 4 cut(s) 319, 1051, 2170, 3269
BstV2I GAAGAC 1 cut(s) 2229
BstX2I RGATCY 1 cut(s) 2147
BstXI CCANNNNNNTGG 1 cut(s) 1139
BstYI RGATCY 1 cut(s) 2147
BstZ17I GTATAC 1 cut(s) 2807
BstZI CGGCCG 1 cut(s) 2466
Bsu15I ATCGAT 2 cut(s) 271, 3394
Bsu36I CCTNAGG 1 cut(s) 3172
BsuTUI ATCGAT 2 cut(s) 271, 3394
BtgI CCRYGG 2 cut(s) 786, 1132
BtrI CACGTC 2 cut(s) 2116, 2197
BtsCI GGATG 6 cut(s) 1080, 1295, 1548, 1953, 2695, 3202
BtsI GCAGTG 2 cut(s) 101, 1783
BtsIMutI CAGTG 9 cut(s) 101, 216, 1371, 1783, 2554, 2622, 2757, 3329, 3336
Cac8I GCNNGC 8 cut(s) 565, 1251, 1527, 1630, 1778, 1792, 1819, 3334
CaiI CAGNNNCTG 2 cut(s) 103, 1018
CciI TCATGA 1 cut(s) 724
CfoI GCGC 5 cut(s) 701, 1790, 1817, 1927, 2142
Cfr10I RCCGGY 1 cut(s) 3213
Cfr13I GGNCC 6 cut(s) 565, 2442, 2791, 3166, 3250, 3269
ClaI ATCGAT 2 cut(s) 271, 3394
CseI GACGC 2 cut(s) 1070, 2210
DpnI GATC 7 cut(s) 150, 2149, 2496, 3178, 3380, 3393, 3397
DpnII GATC 7 cut(s) 148, 2147, 2494, 3176, 3378, 3391, 3395
DraI TTTAAA 1 cut(s) 745
DraIII CACNNNGTG 1 cut(s) 1509
EaeI YGGCCR 4 cut(s) 771, 1399, 1810, 2466
EagI CGGCCG 1 cut(s) 2466
Eam1104I CTCTTC 8 cut(s) 300, 648, 1749, 2079, 2088, 2385, 2394, 2723
EarI CTCTTC 8 cut(s) 300, 648, 1749, 2079, 2088, 2385, 2394, 2723
EciI GGCGGA 4 cut(s) 653, 2052, 2140, 2702
Ecl136II GAGCTC 4 cut(s) 216, 643, 1622, 2042
EclXI CGGCCG 1 cut(s) 2466
Eco130I CCWWGG 5 cut(s) 786, 1117, 1132, 2969, 3289
Eco24I GRGCYC 7 cut(s) 218, 645, 1624, 1869, 2044, 2735, 3054
Eco31I GGTCTC 2 cut(s) 29, 1808
Eco32I GATATC 3 cut(s) 1284, 3067, 3136
Eco47I GGWCC 3 cut(s) 3166, 3250, 3269
Eco47III AGCGCT 1 cut(s) 1926
Eco52I CGGCCG 1 cut(s) 2466
Eco53kI GAGCTC 4 cut(s) 216, 643, 1622, 2042
Eco57I CTGAAG 2 cut(s) 2148, 2279
Eco81I CCTNAGG 1 cut(s) 3172
Eco88I CYCGRG 3 cut(s) 196, 1201, 2060
Eco91I GGTNACC 1 cut(s) 781
EcoICRI GAGCTC 4 cut(s) 216, 643, 1622, 2042
EcoO109I RGGNCCY 1 cut(s) 3250
EcoO65I GGTNACC 1 cut(s) 781
EcoRI GAATTC 1 cut(s) 1273
EcoRII CCWGG 5 cut(s) 567, 1320, 1827, 2243, 2734
EcoRV GATATC 3 cut(s) 1284, 3067, 3136
EcoT14I CCWWGG 5 cut(s) 786, 1117, 1132, 2969, 3289
EcoT38I GRGCYC 7 cut(s) 218, 645, 1624, 1869, 2044, 2735, 3054
ErhI CCWWGG 5 cut(s) 786, 1117, 1132, 2969, 3289
FaqI GGGAC 2 cut(s) 1085, 3325
FauI CCCGC 3 cut(s) 556, 1810, 2151
FblI GTMKAC 3 cut(s) 824, 2806, 2963
Fnu4HI GCNGC 9 cut(s) 333, 636, 1040, 1224, 1813, 2050, 2184, 2501, 3258
FokI GGATG 6 cut(s) 1067, 1302, 1535, 1940, 2702, 3209
FriOI GRGCYC 7 cut(s) 218, 645, 1624, 1869, 2044, 2735, 3054
Fsp4HI GCNGC 9 cut(s) 333, 636, 1040, 1224, 1813, 2050, 2184, 2501, 3258
FspBI CTAG 5 cut(s) 224, 1380, 2970, 3264, 3333
GlaI GCGC 5 cut(s) 700, 1789, 1816, 1926, 2141
GluI GCNGC 9 cut(s) 333, 636, 1040, 1224, 1813, 2050, 2184, 2501, 3258
HaeII RGCGCY 3 cut(s) 702, 1928, 2143
HapII CCGG 7 cut(s) 770, 921, 1350, 2267, 3048, 3075, 3214
HgaI GACGC 2 cut(s) 1070, 2210
HhaI GCGC 5 cut(s) 701, 1790, 1817, 1927, 2142
Hin1I GRCGYC 1 cut(s) 2202
Hin6I GCGC 5 cut(s) 699, 1788, 1815, 1925, 2140
HinP1I GCGC 5 cut(s) 699, 1788, 1815, 1925, 2140
HincII GTYRAC 1 cut(s) 664
HindII GTYRAC 1 cut(s) 664
HpaII CCGG 7 cut(s) 770, 921, 1350, 2267, 3048, 3075, 3214
HphI GGTGA 4 cut(s) 793, 2281, 2650, 3353
Hpy166II GTNNAC 8 cut(s) 664, 825, 1295, 1503, 1882, 2200, 2807, 2964
Hpy8I GTNNAC 8 cut(s) 664, 825, 1295, 1503, 1882, 2200, 2807, 2964
Hpy99I CGWCG 5 cut(s) 505, 2081, 2179, 2182, 2198
HpyCH4III ACNGT 4 cut(s) 1375, 2591, 2961, 3162
HpyCH4IV ACGT 3 cut(s) 585, 2115, 2196
HpyF10VI GCNNNNNNNGC 9 cut(s) 425, 620, 863, 1777, 1818, 2825, 2940, 3058, 3333
HpySE526I ACGT 3 cut(s) 585, 2115, 2196
Hsp92I GRCGYC 1 cut(s) 2202
HspAI GCGC 5 cut(s) 699, 1788, 1815, 1925, 2140
Kpn2I TCCGGA 2 cut(s) 920, 1349
Kzo9I GATC 7 cut(s) 148, 2147, 2494, 3176, 3378, 3391, 3395
LguI GCTCTTC 3 cut(s) 300, 2088, 2723
Lsp1109I GCAGC 4 cut(s) 319, 1051, 2170, 3269
MaeI CTAG 5 cut(s) 224, 1380, 2970, 3264, 3333
MaeII ACGT 3 cut(s) 585, 2115, 2196
MaeIII GTNAC 3 cut(s) 781, 2317, 3085
MalI GATC 7 cut(s) 150, 2149, 2496, 3178, 3380, 3393, 3397
MbiI CCGCTC 2 cut(s) 1487, 2158
MboI GATC 7 cut(s) 148, 2147, 2494, 3176, 3378, 3391, 3395
MfeI CAATTG 3 cut(s) 2477, 3280, 3351
MflI RGATCY 1 cut(s) 2147
MhlI GDGCHC 9 cut(s) 218, 645, 1624, 1705, 1869, 2044, 2735, 3054, 3160
MlsI TGGCCA 1 cut(s) 1401
MluNI TGGCCA 1 cut(s) 1401
MlyI GAGTC 5 cut(s) 830, 1974, 2066, 2230, 3025
MmeI TCCRAC 5 cut(s) 455, 1459, 2094, 2100, 3144
Mox20I TGGCCA 1 cut(s) 1401
MroI TCCGGA 2 cut(s) 920, 1349
MroXI GAANNNNTTC 2 cut(s) 765, 2543
MscI TGGCCA 1 cut(s) 1401
MslI CAYNNNNRTG 5 cut(s) 834, 858, 1137, 1440, 3125
Msp20I TGGCCA 1 cut(s) 1401
MspA1I CMGCKG 2 cut(s) 1042, 2500
MspI CCGG 7 cut(s) 770, 921, 1350, 2267, 3048, 3075, 3214
MspR9I CCNGG 7 cut(s) 569, 1322, 1829, 2245, 2736, 3049, 3076
MunI CAATTG 3 cut(s) 2477, 3280, 3351
Mva1269I GAATGC 3 cut(s) 388, 1028, 2862
MvaI CCWGG 5 cut(s) 569, 1322, 1829, 2245, 2736
MvnI CGCG 3 cut(s) 1815, 1817, 2052
MwoI GCNNNNNNNGC 9 cut(s) 425, 620, 863, 1777, 1818, 2825, 2940, 3058, 3333
NciI CCSGG 2 cut(s) 3049, 3076
NcoI CCATGG 2 cut(s) 786, 1132
NdeII GATC 7 cut(s) 148, 2147, 2494, 3176, 3378, 3391, 3395
NheI GCTAGC 1 cut(s) 3332
NlaIV GGNNCC 8 cut(s) 498, 1483, 1866, 2164, 2443, 3053, 3168, 3287
NmeAIII GCCGAG 3 cut(s) 2179, 2494, 3019
NmuCI GTSAC 1 cut(s) 781
NspI RCATGY 3 cut(s) 1198, 1501, 3007
PaeR7I CTCGAG 2 cut(s) 1201, 2060
PagI TCATGA 1 cut(s) 724
PciI ACATGT 1 cut(s) 1497
PciSI GCTCTTC 3 cut(s) 300, 2088, 2723
PcsI WCGNNNNNNNCGW 2 cut(s) 2067, 3400
PctI GAATGC 3 cut(s) 388, 1028, 2862
PdmI GAANNNNTTC 2 cut(s) 765, 2543
PfeI GAWTC 8 cut(s) 238, 572, 578, 917, 1166, 2254, 2263, 2693
PfoI TCCNGGA 2 cut(s) 1827, 2243
PkrI GCNGC 9 cut(s) 334, 637, 1041, 1225, 1814, 2051, 2185, 2502, 3259
Ple19I CGATCG 1 cut(s) 3394
PleI GAGTC 5 cut(s) 829, 1974, 2066, 2229, 3024
PpsI GAGTC 5 cut(s) 829, 1974, 2066, 2229, 3024
PpuMI RGGWCCY 1 cut(s) 3250
PscI ACATGT 1 cut(s) 1497
Psp124BI GAGCTC 4 cut(s) 218, 645, 1624, 2044
Psp5II RGGWCCY 1 cut(s) 3250
Psp6I CCWGG 5 cut(s) 567, 1320, 1827, 2243, 2734
PspEI GGTNACC 1 cut(s) 781
PspGI CCWGG 5 cut(s) 567, 1320, 1827, 2243, 2734
PspN4I GGNNCC 8 cut(s) 498, 1483, 1866, 2164, 2443, 3053, 3168, 3287
PspPI GGNCC 6 cut(s) 565, 2442, 2791, 3166, 3250, 3269
PspPPI RGGWCCY 1 cut(s) 3250
PspXI VCTCGAGB 2 cut(s) 1201, 2060
PstI CTGCAG 1 cut(s) 625
PstNI CAGNNNCTG 2 cut(s) 103, 1018
PsuI RGATCY 1 cut(s) 2147
PvuI CGATCG 1 cut(s) 3394
RseI CAYNNNNRTG 5 cut(s) 834, 858, 1137, 1440, 3125
SacI GAGCTC 4 cut(s) 218, 645, 1624, 2044
SapI GCTCTTC 3 cut(s) 300, 2088, 2723
SatI GCNGC 9 cut(s) 333, 636, 1040, 1224, 1813, 2050, 2184, 2501, 3258
Sau3AI GATC 7 cut(s) 148, 2147, 2494, 3176, 3378, 3391, 3395
Sau96I GGNCC 6 cut(s) 565, 2442, 2791, 3166, 3250, 3269
ScaI AGTACT 1 cut(s) 289
SchI GAGTC 5 cut(s) 830, 1974, 2066, 2230, 3025
ScrFI CCNGG 7 cut(s) 569, 1322, 1829, 2245, 2736, 3049, 3076
SduI GDGCHC 9 cut(s) 218, 645, 1624, 1705, 1869, 2044, 2735, 3054, 3160
SfcI CTRYAG 1 cut(s) 621
Sfr274I CTCGAG 2 cut(s) 1201, 2060
SinI GGWCC 3 cut(s) 3166, 3250, 3269
SlaI CTCGAG 2 cut(s) 1201, 2060
SmiMI CAYNNNNRTG 5 cut(s) 834, 858, 1137, 1440, 3125
SmlI CTYRAG 5 cut(s) 1201, 2060, 2210, 2225, 3304
SmoI CTYRAG 5 cut(s) 1201, 2060, 2210, 2225, 3304
SspI AATATT 1 cut(s) 1736
SspMI CTAG 5 cut(s) 224, 1380, 2970, 3264, 3333
SstI GAGCTC 4 cut(s) 218, 645, 1624, 2044
StyD4I CCNGG 7 cut(s) 567, 1320, 1827, 2243, 2734, 3047, 3074
StyI CCWWGG 5 cut(s) 786, 1117, 1132, 2969, 3289
TaaI ACNGT 4 cut(s) 1375, 2591, 2961, 3162
TaiI ACGT 3 cut(s) 588, 2118, 2199
TaqII GACCGA 2 cut(s) 11, 2686
TatI WGTACW 6 cut(s) 129, 287, 1197, 1294, 1500, 2580
TauI GCSGC 5 cut(s) 638, 1226, 1815, 2052, 2503
TfiI GAWTC 8 cut(s) 238, 572, 578, 917, 1166, 2254, 2263, 2693
TscAI CASTG 9 cut(s) 108, 216, 1378, 1790, 2554, 2629, 2757, 3329, 3343
TseFI GTSAC 1 cut(s) 781
TseI GCWGC 4 cut(s) 332, 1039, 2183, 3257
Tsp45I GTSAC 1 cut(s) 781
TspGWI ACGGA 2 cut(s) 489, 1905
TspRI CASTG 9 cut(s) 108, 216, 1378, 1790, 2554, 2629, 2757, 3329, 3343
VpaK11BI GGWCC 3 cut(s) 3166, 3250, 3269
XapI RAATTY 8 cut(s) 1273, 1933, 1940, 2405, 2767, 2878, 2917, 2950
XceI RCATGY 3 cut(s) 1198, 1501, 3007
XcmI CCANNNNNNNNNTGG 1 cut(s) 2310
XhoI CTCGAG 2 cut(s) 1201, 2060
XmaJI CCTAGG 1 cut(s) 2969
XmiI GTMKAC 3 cut(s) 824, 2806, 2963
XmnI GAANNNNTTC 2 cut(s) 765, 2543
XspI CTAG 5 cut(s) 224, 1380, 2970, 3264, 3333
ZrmI AGTACT 1 cut(s) 289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.