pycom15g08930

Coiled-coil domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0013078
Physical Location & Seq
Reverse (-)
5745780 .. 5747818
2039 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 651 bp
ATGGTGTTCTACTTCAAAGCGCGATCGGACGTGGGCGATTTCACCATTTTCATGGGCCTCGACAAGTTCGAGAACGAGGAGCTCATCAAATATGGCTTCCCCGAAGACATTTGGTTCCATGTGGACAAAATGTCTTCCGCCCATGTGTATGTTAGGCTGCACAAAGGACAGACCATCGATGATATAAGTGAAGGTTTACTGGAGGATTGTGCTCAGCTTGTCAAAGCAAATTCCATCCAAGGCAACAAGGTAAACAATGTTGATGTTGTTTACACTCCCTGGTCCAATTTGAAGAAAAGTGCCTCCATGGATGTTGGTCAAGTTGGTTTCTACAATTCAAAGAATGTTCGGACTGTGAGGGTGGAGAAGCGATCGATCAATGAGGTAGTTAATAGATTGAACAGGACAAAAGTAGAAAGGAAGCCTGATTTGAAAGCTGAGCGAGAAGCTGTTAATGCAGCCGAAAGAGCTGAGAGAAAGCAGTTCCTGAGGGACAAGAAACGACGTGAGGAGATGGAAAGGCTTGAGAAGGAGAGACAATTGGAACTAAGGAGCTACAAAGGTTTGATGAATTCTGAAAATATGACATCGAACAAACAAATTGCATCGGCAAACAAGTCATTGCAGGAACTTGAAGAGGACTTCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF814

Protein Analysis

217

Amino Acids

25.24

Weight (kDa)

8.49

Isoelectric Point (pI)

39.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NFACT-R_1 PF05670 1 - 112 5.4e-36 NFACT protein RNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013078)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G11500 AT5G11500
fragaria_vesca FvH4_2g36060
malus_domestica MD08G1117900.v1.1 MD15G1097400.v1.1
prunus_persica Prupe.1G452200_v2.0.a1 Prupe.1G452200_v2.0.a1
pyrus_communis pycom08g09850 pycom15g08930
rosa_chinensis RchiOBHm_Chr6g0305791
rosa_laevigata RLG00000010858
rosa_roxburghii Rroxscaffold_7G00162330
rosa_rugosa Rorug06G0344000
rosa_samantha Rh6AG455600 Rh6BG445500 Rh6CG469600 Rh6DG456300
rosa_wichuraiana Rw6G039750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 22
AciI CCGC 1 cut(s) 138
AcsI RAATTY 2 cut(s) 229, 571
AgsI TTSAA 6 cut(s) 16, 292, 339, 400, 433, 635
AhdI GACNNNNNGTC 1 cut(s) 130
AjiI CACGTC 2 cut(s) 31, 506
AjnI CCWGG 1 cut(s) 278
AluBI AGCT 6 cut(s) 82, 217, 437, 449, 470, 555
AluI AGCT 6 cut(s) 82, 217, 437, 449, 470, 555
Alw21I GWGCWC 2 cut(s) 84, 214
Alw26I GTCTC 1 cut(s) 529
AlwNI CAGNNNCTG 1 cut(s) 487
AoxI GGCC 1 cut(s) 55
ApeKI GCWGC 2 cut(s) 157, 458
ApoI RAATTY 2 cut(s) 229, 571
AspLEI GCGC 1 cut(s) 22
AspS9I GGNCC 2 cut(s) 55, 282
AsuHPI GGTGA 1 cut(s) 34
AvaII GGWCC 1 cut(s) 282
AxyI CCTNAGG 1 cut(s) 488
BanII GRGCYC 1 cut(s) 84
BbsI GAAGAC 2 cut(s) 111, 126
Bbv12I GWGCWC 2 cut(s) 84, 214
BbvI GCAGC 2 cut(s) 144, 470
BccI CCATC 3 cut(s) 182, 242, 508
BciT130I CCWGG 1 cut(s) 280
BcoDI GTCTC 1 cut(s) 529
BisI GCNGC 2 cut(s) 158, 459
BlpI GCTNAGC 2 cut(s) 213, 438
BlsI GCNGC 2 cut(s) 159, 460
Bme1390I CCNGG 1 cut(s) 280
Bme18I GGWCC 1 cut(s) 282
BmeRI GACNNNNNGTC 1 cut(s) 130
BmgBI CACGTC 2 cut(s) 31, 506
BmgT120I GGNCC 2 cut(s) 55, 282
BmiI GGNNCC 1 cut(s) 116
BmrFI CCNGG 1 cut(s) 280
BmsI GCATC 1 cut(s) 614
BpiI GAAGAC 2 cut(s) 111, 126
BpmI CTGGAG 1 cut(s) 221
Bpu1102I GCTNAGC 2 cut(s) 213, 438
BpuEI CTTGAG 1 cut(s) 545
Bsa29I ATCGAT 2 cut(s) 177, 374
BsaJI CCNNGG 3 cut(s) 238, 278, 306
Bse1I ACTGG 1 cut(s) 204
Bse21I CCTNAGG 1 cut(s) 488
Bse3DI GCAATG 1 cut(s) 620
BseBI CCWGG 1 cut(s) 280
BseCI ATCGAT 2 cut(s) 177, 374
BseDI CCNNGG 3 cut(s) 238, 278, 306
BseGI GGATG 2 cut(s) 234, 316
BseMI GCAATG 1 cut(s) 620
BseMII CTCAG 4 cut(s) 227, 429, 462, 479
BseNI ACTGG 1 cut(s) 204
BseRI GAGGAG 2 cut(s) 92, 524
BseXI GCAGC 2 cut(s) 144, 470
BsgI GTGCAG 1 cut(s) 143
Bsh1236I CGCG 1 cut(s) 22
Bsh1285I CGRYCG 2 cut(s) 26, 374
BshFI GGCC 1 cut(s) 57
BshVI ATCGAT 2 cut(s) 177, 374
BsiEI CGRYCG 2 cut(s) 26, 374
BsiHKAI GWGCWC 2 cut(s) 84, 214
BslFI GGGAC 1 cut(s) 506
BsmAI GTCTC 1 cut(s) 529
BsmFI GGGAC 1 cut(s) 506
BsnI GGCC 1 cut(s) 57
Bsp1286I GDGCHC 2 cut(s) 84, 214
Bsp143I GATC 3 cut(s) 23, 371, 375
Bsp1720I GCTNAGC 2 cut(s) 213, 438
Bsp19I CCATGG 1 cut(s) 306
BspACI CCGC 1 cut(s) 138
BspANI GGCC 1 cut(s) 57
BspCNI CTCAG 4 cut(s) 226, 430, 463, 480
BspDI ATCGAT 2 cut(s) 177, 374
BspFNI CGCG 1 cut(s) 22
BspLI GGNNCC 1 cut(s) 116
BsrDI GCAATG 1 cut(s) 620
BsrI ACTGG 1 cut(s) 204
BssECI CCNNGG 3 cut(s) 238, 278, 306
BssMI GATC 3 cut(s) 23, 371, 375
BssT1I CCWWGG 2 cut(s) 238, 306
Bst2UI CCWGG 1 cut(s) 280
Bst4CI ACNGT 1 cut(s) 355
Bst6I CTCTTC 1 cut(s) 630
BstDEI CTNAG 5 cut(s) 213, 438, 471, 488, 548
BstDSI CCRYGG 1 cut(s) 306
BstF5I GGATG 2 cut(s) 234, 316
BstFNI CGCG 1 cut(s) 22
BstHHI GCGC 1 cut(s) 22
BstKTI GATC 3 cut(s) 26, 374, 378
BstMAI GTCTC 1 cut(s) 529
BstMBI GATC 3 cut(s) 23, 371, 375
BstMCI CGRYCG 2 cut(s) 26, 374
BstMWI GCNNNNNNNGC 2 cut(s) 455, 467
BstNI CCWGG 1 cut(s) 280
BstSCI CCNGG 1 cut(s) 278
BstUI CGCG 1 cut(s) 22
BstV1I GCAGC 2 cut(s) 144, 470
BstV2I GAAGAC 2 cut(s) 111, 126
BstXI CCANNNNNNTGG 1 cut(s) 52
Bsu15I ATCGAT 2 cut(s) 177, 374
Bsu36I CCTNAGG 1 cut(s) 488
BsuRI GGCC 1 cut(s) 57
BsuTUI ATCGAT 2 cut(s) 177, 374
BtgI CCRYGG 1 cut(s) 306
BtrI CACGTC 2 cut(s) 31, 506
BtsCI GGATG 2 cut(s) 234, 316
CaiI CAGNNNCTG 1 cut(s) 487
CfoI GCGC 1 cut(s) 22
Cfr13I GGNCC 2 cut(s) 55, 282
ClaI ATCGAT 2 cut(s) 177, 374
CviAII CATG 5 cut(s) 52, 119, 143, 307, 646
DdeI CTNAG 5 cut(s) 213, 438, 471, 488, 548
DpnI GATC 3 cut(s) 25, 373, 377
DpnII GATC 3 cut(s) 23, 371, 375
DriI GACNNNNNGTC 1 cut(s) 130
Eam1104I CTCTTC 1 cut(s) 630
Eam1105I GACNNNNNGTC 1 cut(s) 130
EarI CTCTTC 1 cut(s) 630
EciI GGCGGA 1 cut(s) 127
Ecl136II GAGCTC 1 cut(s) 82
Eco130I CCWWGG 2 cut(s) 238, 306
Eco24I GRGCYC 1 cut(s) 84
Eco47I GGWCC 1 cut(s) 282
Eco53kI GAGCTC 1 cut(s) 82
Eco81I CCTNAGG 1 cut(s) 488
EcoICRI GAGCTC 1 cut(s) 82
EcoRI GAATTC 1 cut(s) 571
EcoRII CCWGG 1 cut(s) 278
EcoT14I CCWWGG 2 cut(s) 238, 306
EcoT38I GRGCYC 1 cut(s) 84
ErhI CCWWGG 2 cut(s) 238, 306
FaeI CATG 5 cut(s) 55, 122, 146, 310, 649
FaiI YATR 9 cut(s) 53, 93, 120, 144, 150, 185, 308, 584, 647
FaqI GGGAC 1 cut(s) 506
FatI CATG 5 cut(s) 51, 118, 142, 306, 645
Fnu4HI GCNGC 2 cut(s) 158, 459
FokI GGATG 2 cut(s) 221, 323
FriOI GRGCYC 1 cut(s) 84
Fsp4HI GCNGC 2 cut(s) 158, 459
GlaI GCGC 1 cut(s) 21
GluI GCNGC 2 cut(s) 158, 459
GsuI CTGGAG 1 cut(s) 221
HaeIII GGCC 1 cut(s) 57
HhaI GCGC 1 cut(s) 22
Hin1II CATG 5 cut(s) 55, 122, 146, 310, 649
Hin6I GCGC 1 cut(s) 20
HinP1I GCGC 1 cut(s) 20
HphI GGTGA 1 cut(s) 34
Hpy166II GTNNAC 4 cut(s) 124, 197, 253, 271
Hpy188I TCNGA 3 cut(s) 28, 351, 577
Hpy188III TCNNGA 2 cut(s) 70, 487
Hpy8I GTNNAC 4 cut(s) 124, 197, 253, 271
Hpy99I CGWCG 1 cut(s) 507
HpyAV CCTTC 2 cut(s) 185, 523
HpyCH4III ACNGT 1 cut(s) 355
HpyCH4IV ACGT 2 cut(s) 30, 505
HpyCH4V TGCA 4 cut(s) 160, 458, 605, 625
HpyF10VI GCNNNNNNNGC 2 cut(s) 455, 467
HpyF3I CTNAG 5 cut(s) 213, 438, 471, 488, 548
HpySE526I ACGT 2 cut(s) 30, 505
Hsp92II CATG 5 cut(s) 55, 122, 146, 310, 649
HspAI GCGC 1 cut(s) 20
Kzo9I GATC 3 cut(s) 23, 371, 375
LmnI GCTCC 2 cut(s) 79, 552
LpnPI CCDG 7 cut(s) 185, 265, 292, 388, 438, 500, 611
Lsp1109I GCAGC 2 cut(s) 144, 470
LweI GCATC 1 cut(s) 614
MaeII ACGT 2 cut(s) 30, 505
MalI GATC 3 cut(s) 25, 373, 377
MboI GATC 3 cut(s) 23, 371, 375
MboII GAAGA 4 cut(s) 116, 126, 304, 647
MfeI CAATTG 1 cut(s) 539
MhlI GDGCHC 2 cut(s) 84, 214
MluCI AATT 6 cut(s) 229, 286, 334, 539, 571, 600
MnlI CCTC 9 cut(s) 68, 70, 196, 313, 351, 376, 483, 502, 631
MseI TTAA 2 cut(s) 390, 453
MslI CAYNNNNRTG 2 cut(s) 50, 147
MspR9I CCNGG 1 cut(s) 280
MunI CAATTG 1 cut(s) 539
MvaI CCWGG 1 cut(s) 280
MvnI CGCG 1 cut(s) 22
MwoI GCNNNNNNNGC 2 cut(s) 455, 467
NcoI CCATGG 1 cut(s) 306
NdeII GATC 3 cut(s) 23, 371, 375
NlaIII CATG 5 cut(s) 55, 122, 146, 310, 649
NlaIV GGNNCC 1 cut(s) 116
PcsI WCGNNNNNNNCGW 1 cut(s) 66
PkrI GCNGC 2 cut(s) 159, 460
Ple19I CGATCG 2 cut(s) 26, 374
Psp124BI GAGCTC 1 cut(s) 84
Psp6I CCWGG 1 cut(s) 278
PspGI CCWGG 1 cut(s) 278
PspN4I GGNNCC 1 cut(s) 116
PspPI GGNCC 2 cut(s) 55, 282
PstNI CAGNNNCTG 1 cut(s) 487
PvuI CGATCG 2 cut(s) 26, 374
RseI CAYNNNNRTG 2 cut(s) 50, 147
SacI GAGCTC 1 cut(s) 84
SaqAI TTAA 2 cut(s) 390, 453
SatI GCNGC 2 cut(s) 158, 459
Sau3AI GATC 3 cut(s) 23, 371, 375
Sau96I GGNCC 2 cut(s) 55, 282
ScrFI CCNGG 1 cut(s) 280
SduI GDGCHC 2 cut(s) 84, 214
SfaNI GCATC 1 cut(s) 614
SinI GGWCC 1 cut(s) 282
SmiMI CAYNNNNRTG 2 cut(s) 50, 147
SmlI CTYRAG 1 cut(s) 524
SmoI CTYRAG 1 cut(s) 524
Sse9I AATT 6 cut(s) 229, 286, 334, 539, 571, 600
SsiI CCGC 1 cut(s) 138
SstI GAGCTC 1 cut(s) 84
StyD4I CCNGG 1 cut(s) 278
StyI CCWWGG 2 cut(s) 238, 306
TaaI ACNGT 1 cut(s) 355
TaiI ACGT 2 cut(s) 33, 508
TaqI TCGA 5 cut(s) 60, 69, 177, 374, 590
TasI AATT 6 cut(s) 229, 286, 334, 539, 571, 600
Tru1I TTAA 2 cut(s) 390, 453
Tru9I TTAA 2 cut(s) 390, 453
TseI GCWGC 2 cut(s) 157, 458
TspDTI ATGAA 3 cut(s) 40, 584, 634
VpaK11BI GGWCC 1 cut(s) 282
XapI RAATTY 2 cut(s) 229, 571
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.