pycom15g09340

EF-hand domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0016772
Physical Location & Seq
Forward (+)
6095098 .. 6095667
570 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 570 bp
ATGGGAATTACAGGAGTTTGCTTTAGATTACTTGGTGACTTGGTTCAAGCCATTGGAGGAGTCCCATCAAGTTCAACAAGCAAAGGAGCTCACATTGTTCATGAAGATCAACGTGACCAATATTCCAAATGCAAACCACAAAATATTGCAACCAAAGTTGATGAGTCCATGGCAAGGGCACTGATCACAGTGTTTGGGATGGAAACAAATGGAAGAATCAAGAAGGAAAAGGCGCGGCTTGTCGTGGAGAATCTTGGACTAATACGTAATGAGGAAGACCAGACGGGTTTCGATTTGCCAGGGGATGATCATGAAGTGCCAGTGGAGGAGGTCCTTGGTGGATTGGAGGATAATGAGTCCGAGCGCAACGAGCTCCTGCGCGAAGCATTCAAGATTTTCGACGAGGATGGCAATGGATTCATAGAGGCTGTGGAGTTGAAAAGGGTTTTGGAATGCTTGGGTTTGGGGAGTGGGTGGGAGATGGACCAGGTTGAGAAGATGGTGAAGGTTGTGGATTTGAATATGGATGGGAAGGTTGATTTTCGAGAGTTTGAGTTAATGATGGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0008150 GO:0010035 GO:0010038 GO:0042221 GO:0043167 GO:0043169 GO:0046686 GO:0046872 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7

Protein Analysis

190

Amino Acids

21.03

Weight (kDa)

4.59

Isoelectric Point (pI)

34.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 126 - 188 3e-13 EF-hand domain pair
EF-hand_1 PF00036 126 - 151 2.6e-06 EF hand domain
EF-hand_6 PF13405 126 - 154 4.1e-08 EF-hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016772)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g36371
malus_domestica MD08G1123200.v1.1 MD15G1102200.v1.1
prunus_persica Prupe.1G455200_v2.0.a1
pyrus_communis pycom15g09340
rosa_laevigata RLG00000010883
rosa_multiflora Rmu_sc0001809.1_g000003
rosa_roxburghii Rroxscaffold_7G00162730
rosa_rugosa Rorug06G0340900
rosa_samantha Rh6AG453100 Rh6BG448700 Rh6CG466800 Rh6DG453500
rosa_wichuraiana Rw6G039480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 235, 381
AciI CCGC 1 cut(s) 235
AfiI CCNNNNNNNGG 1 cut(s) 174
AgsI TTSAA 5 cut(s) 47, 75, 391, 439, 520
AjnI CCWGG 2 cut(s) 298, 486
AjuI GAANNNNNNNTTGG 2 cut(s) 431, 463
AluBI AGCT 2 cut(s) 89, 373
AluI AGCT 2 cut(s) 89, 373
Alw21I GWGCWC 2 cut(s) 91, 375
AspLEI GCGC 3 cut(s) 235, 366, 381
AspS9I GGNCC 2 cut(s) 331, 484
AsuHPI GGTGA 2 cut(s) 47, 514
AvaII GGWCC 2 cut(s) 331, 484
BaeGI GKGCMC 1 cut(s) 181
BanII GRGCYC 2 cut(s) 91, 375
BbsI GAAGAC 1 cut(s) 282
Bbv12I GWGCWC 2 cut(s) 91, 375
BccI CCATC 7 cut(s) 73, 193, 401, 475, 493, 521, 556
BciT130I CCWGG 2 cut(s) 300, 488
BclI TGATCA 2 cut(s) 183, 307
BisI GCNGC 1 cut(s) 236
BlsI GCNGC 1 cut(s) 237
Bme1390I CCNGG 2 cut(s) 300, 488
Bme18I GGWCC 2 cut(s) 331, 484
BmgT120I GGNCC 2 cut(s) 331, 484
BmrFI CCNGG 2 cut(s) 300, 488
BpiI GAAGAC 1 cut(s) 282
BsaAI YACGTR 1 cut(s) 266
BsaJI CCNNGG 3 cut(s) 168, 299, 334
Bsc4I CCNNNNNNNGG 1 cut(s) 174
Bse1I ACTGG 1 cut(s) 320
Bse3DI GCAATG 1 cut(s) 418
BseBI CCWGG 2 cut(s) 300, 488
BseDI CCNNGG 3 cut(s) 168, 299, 334
BseGI GGATG 4 cut(s) 204, 310, 412, 532
BseLI CCNNNNNNNGG 1 cut(s) 174
BseMI GCAATG 1 cut(s) 418
BseNI ACTGG 1 cut(s) 320
BseRI GAGGAG 2 cut(s) 72, 341
BseSI GKGCMC 1 cut(s) 181
Bsh1236I CGCG 2 cut(s) 235, 381
BsiHKAI GWGCWC 2 cut(s) 91, 375
BslFI GGGAC 1 cut(s) 47
BslI CCNNNNNNNGG 1 cut(s) 174
BsmFI GGGAC 1 cut(s) 47
BsmI GAATGC 2 cut(s) 386, 458
Bsp1286I GDGCHC 3 cut(s) 91, 181, 375
Bsp143I GATC 3 cut(s) 106, 183, 307
Bsp19I CCATGG 1 cut(s) 168
BspACI CCGC 1 cut(s) 235
BspFNI CGCG 2 cut(s) 235, 381
BspHI TCATGA 2 cut(s) 100, 310
BsrDI GCAATG 1 cut(s) 418
BsrI ACTGG 1 cut(s) 320
BssECI CCNNGG 3 cut(s) 168, 299, 334
BssMI GATC 3 cut(s) 106, 183, 307
BssT1I CCWWGG 2 cut(s) 168, 334
Bst2UI CCWGG 2 cut(s) 300, 488
Bst4CI ACNGT 1 cut(s) 190
BstBAI YACGTR 1 cut(s) 266
BstDSI CCRYGG 1 cut(s) 168
BstF5I GGATG 4 cut(s) 204, 310, 412, 532
BstFNI CGCG 2 cut(s) 235, 381
BstHHI GCGC 3 cut(s) 235, 366, 381
BstKTI GATC 3 cut(s) 109, 186, 310
BstMBI GATC 3 cut(s) 106, 183, 307
BstMWI GCNNNNNNNGC 1 cut(s) 370
BstNI CCWGG 2 cut(s) 300, 488
BstSCI CCNGG 2 cut(s) 298, 486
BstSLI GKGCMC 1 cut(s) 181
BstSNI TACGTA 1 cut(s) 266
BstUI CGCG 2 cut(s) 235, 381
BstV2I GAAGAC 1 cut(s) 282
BtgI CCRYGG 1 cut(s) 168
BtsCI GGATG 4 cut(s) 204, 310, 412, 532
BtsIMutI CAGTG 3 cut(s) 179, 195, 327
CciI TCATGA 2 cut(s) 100, 310
CfoI GCGC 3 cut(s) 235, 366, 381
Cfr13I GGNCC 2 cut(s) 331, 484
CsiI ACCWGGT 1 cut(s) 486
CviAII CATG 3 cut(s) 101, 169, 311
CviJI RGCY 5 cut(s) 50, 89, 238, 373, 428
CviKI_1 RGCY 5 cut(s) 50, 89, 238, 373, 428
DpnI GATC 3 cut(s) 108, 185, 309
DpnII GATC 3 cut(s) 106, 183, 307
Ecl136II GAGCTC 2 cut(s) 89, 373
Eco105I TACGTA 1 cut(s) 266
Eco130I CCWWGG 2 cut(s) 168, 334
Eco24I GRGCYC 2 cut(s) 91, 375
Eco47I GGWCC 2 cut(s) 331, 484
Eco53kI GAGCTC 2 cut(s) 89, 373
EcoICRI GAGCTC 2 cut(s) 89, 373
EcoO109I RGGNCCY 1 cut(s) 331
EcoRII CCWGG 2 cut(s) 298, 486
EcoT14I CCWWGG 2 cut(s) 168, 334
EcoT38I GRGCYC 2 cut(s) 91, 375
ErhI CCWWGG 2 cut(s) 168, 334
FaeI CATG 3 cut(s) 104, 172, 314
FaiI YATR 5 cut(s) 102, 170, 312, 422, 524
FaqI GGGAC 1 cut(s) 47
FatI CATG 3 cut(s) 100, 168, 310
FbaI TGATCA 2 cut(s) 183, 307
Fnu4HI GCNGC 1 cut(s) 236
FokI GGATG 4 cut(s) 211, 317, 419, 539
FriOI GRGCYC 2 cut(s) 91, 375
Fsp4HI GCNGC 1 cut(s) 236
GlaI GCGC 3 cut(s) 234, 365, 380
GluI GCNGC 1 cut(s) 236
HhaI GCGC 3 cut(s) 235, 366, 381
Hin1II CATG 3 cut(s) 104, 172, 314
Hin6I GCGC 3 cut(s) 233, 364, 379
HinP1I GCGC 3 cut(s) 233, 364, 379
HinfI GANTC 6 cut(s) 60, 164, 216, 250, 356, 417
HphI GGTGA 2 cut(s) 47, 514
Hpy188I TCNGA 1 cut(s) 361
Hpy188III TCNNGA 5 cut(s) 101, 220, 311, 391, 545
Hpy99I CGWCG 1 cut(s) 404
HpyAV CCTTC 3 cut(s) 217, 499, 526
HpyCH4III ACNGT 1 cut(s) 190
HpyCH4IV ACGT 2 cut(s) 112, 265
HpyCH4V TGCA 2 cut(s) 132, 149
HpyF10VI GCNNNNNNNGC 1 cut(s) 370
HpySE526I ACGT 2 cut(s) 112, 265
Hsp92II CATG 3 cut(s) 104, 172, 314
HspAI GCGC 3 cut(s) 233, 364, 379
Ksp22I TGATCA 2 cut(s) 183, 307
Kzo9I GATC 3 cut(s) 106, 183, 307
LmnI GCTCC 2 cut(s) 86, 378
LpnPI CCDG 7 cut(s) 285, 293, 312, 333, 389, 473, 500
MabI ACCWGGT 1 cut(s) 486
MaeII ACGT 2 cut(s) 112, 265
MaeIII GTNAC 2 cut(s) 35, 113
MalI GATC 3 cut(s) 108, 185, 309
MboI GATC 3 cut(s) 106, 183, 307
MboII GAAGA 4 cut(s) 116, 225, 287, 508
MhlI GDGCHC 3 cut(s) 91, 181, 375
MluCI AATT 1 cut(s) 6
MlyI GAGTC 3 cut(s) 69, 173, 365
MnlI CCTC 7 cut(s) 50, 265, 319, 322, 340, 397, 418
MseI TTAA 1 cut(s) 557
MspR9I CCNGG 2 cut(s) 300, 488
Mva1269I GAATGC 2 cut(s) 386, 458
MvaI CCWGG 2 cut(s) 300, 488
MvnI CGCG 2 cut(s) 235, 381
MwoI GCNNNNNNNGC 1 cut(s) 370
NcoI CCATGG 1 cut(s) 168
NdeII GATC 3 cut(s) 106, 183, 307
NlaIII CATG 3 cut(s) 104, 172, 314
NmuCI GTSAC 2 cut(s) 35, 113
PagI TCATGA 2 cut(s) 100, 310
PctI GAATGC 2 cut(s) 386, 458
PfeI GAWTC 3 cut(s) 216, 250, 417
PkrI GCNGC 1 cut(s) 237
PleI GAGTC 3 cut(s) 68, 172, 364
PpsI GAGTC 3 cut(s) 68, 172, 364
Ppu21I YACGTR 1 cut(s) 266
PpuMI RGGWCCY 1 cut(s) 331
Psp124BI GAGCTC 2 cut(s) 91, 375
Psp5II RGGWCCY 1 cut(s) 331
Psp6I CCWGG 2 cut(s) 298, 486
PspGI CCWGG 2 cut(s) 298, 486
PspPI GGNCC 2 cut(s) 331, 484
PspPPI RGGWCCY 1 cut(s) 331
SacI GAGCTC 2 cut(s) 91, 375
SaqAI TTAA 1 cut(s) 557
SatI GCNGC 1 cut(s) 236
Sau3AI GATC 3 cut(s) 106, 183, 307
Sau96I GGNCC 2 cut(s) 331, 484
SchI GAGTC 3 cut(s) 69, 173, 365
ScrFI CCNGG 2 cut(s) 300, 488
SduI GDGCHC 3 cut(s) 91, 181, 375
SetI ASST 8 cut(s) 91, 115, 268, 333, 375, 492, 510, 537
SexAI ACCWGGT 1 cut(s) 486
SinI GGWCC 2 cut(s) 331, 484
SnaBI TACGTA 1 cut(s) 266
Sse9I AATT 1 cut(s) 6
SsiI CCGC 1 cut(s) 235
SspI AATATT 2 cut(s) 122, 145
SstI GAGCTC 2 cut(s) 91, 375
StyD4I CCNGG 2 cut(s) 298, 486
StyI CCWWGG 2 cut(s) 168, 334
TaaI ACNGT 1 cut(s) 190
TaiI ACGT 2 cut(s) 115, 268
TaqI TCGA 3 cut(s) 291, 399, 544
TasI AATT 1 cut(s) 6
TauI GCSGC 1 cut(s) 238
TfiI GAWTC 3 cut(s) 216, 250, 417
Tru1I TTAA 1 cut(s) 557
Tru9I TTAA 1 cut(s) 557
TscAI CASTG 3 cut(s) 186, 195, 327
TseFI GTSAC 2 cut(s) 35, 113
Tsp45I GTSAC 2 cut(s) 35, 113
TspDTI ATGAA 4 cut(s) 89, 117, 327, 409
TspRI CASTG 3 cut(s) 186, 195, 327
VpaK11BI GGWCC 2 cut(s) 331, 484
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.