Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0012549

Physical Location & Seq

Genomic Coordinates Forward (+)

6615815 .. 6618442

Gene Structure 2628 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1473 bp

ATGGGGTTGCTGAAAGGTGTGGACTTTACAGGCCTAAGTGTTGCCTTATTGTTCTTCAATCTGCTGGTGGGGTTAGTGGTTGGGATTGGAGCGAACTGGGGGACACAGTTAACACACCCTCTGCCACCAGCGACTGTTGTGAAGATGTTGAAGGACAACGGGATCCAAAAGGTCAAGCTTTTCGATGCCGATTCGGGTATTCTGAATGCTCTGAGCAAGTCTGGGATTCAGGTCATGGTTGGGATCCCCAATGAGATGCTGTATTCTTTGTCTAACAGTATGCAGGCAGCTGAGAATTGGGTTTCCAAGAATGTCTCCTCACATGTATCCAACGGTGTAGATATCAGGTATGCTGCAGTGGGGAATGAACCATTGTTGTCAACATACAATGGAACTTTTCTTCCAACAATATTTCCTGCCCTTCAGAATATCCAGTCAGCTCTCATAAAAGCCGGTGTGAGCAATCAGGTTAAGGTCACGATTCCTATCAACGCTGACGTCTACGACAGTGGTGGCTCAGACCTACCTTCTAATGGAGACTTTCGACCAGACACCAAGGATCTCATGTTGGAAATTGTCAAGTTCCTGAGCGACAACGGTGCTGCATTCACTGTCAATATATATCCCTTCATAAGCCTCTATAATGATGCCAATTTTCCAGCTGATTACGCCTTCTTCAATGGATACTCGAGTCCTATTAACGATAATGGAAGAATCTACAGCAATGTGTTTGATGCAAACCATGACACCCTTGTATGGGCGTTACAGAAAAATGGATTTGGAAACTTGTCCATAATTGTTGGAGAGATCGGTTGGCCTACAGATGGGGACCTGAATGCAAATGTACAGACTGCGCAACGGTTCAACCAAGGGTTTATGTCCCATATCAAGAATAACGCAGGGACTCCAATGAGAGCCGGCCCTGTGGACGCCTACTTATTTAGCCTCATCGATGAAGATGCCAAGAGCATTCAGCCAGGTAACTTTGAACGCCATTGGGGGCTGTTTTACTGGGACGGAACACCTAAGTATCAGCTCAGTCTAGGCACTACAGGCTCGAATGGATTAGTAGCAGCAAGTGGCGTACAATATCTGCAAAAGAAATGGTGTGTCATGTCGCCGTCAGCGAGTCTTGATGATCCAGAAGTGGCTCTAAGCGTGAGCTATGCATGTTCAAATGCTGATTGCACTAGTCTTGGATATGGAACTTCATGTGGAGATTTGGATGCTCGCGGAAACATTTCGTATGCATTTAACAGTTACTATCAAATAAATAATCAGCTGGACACTGCCTGCAAGTTTCCAAACCTTTCGACTGTCGCCACGGGTGATCGATCCACTGGAAACTGCAAATTTAGGATCATGATCCGAGCATCTTCTTCGTCTAGTGAGAACGCCGGAGCTGGCTCTCTTGAAAAGCCTAGTTGTTTAGGAATGGTGTTTGTGTTTATGTTCCTGTATACAATTCTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001871 GO:0003674 GO:0003824 GO:0004553 GO:0005488 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0005911 GO:0009505 GO:0009506 GO:0016020 GO:0016787 GO:0016798 GO:0030054 GO:0030246 GO:0030247 GO:0030312 GO:0031224 GO:0031225 GO:0031226 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0046658 GO:0055044 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00500 map00500

Pfam Domains

Protein Families

Glyco_hydro_17 X8

Protein Analysis

491

Amino Acids

52.78

Weight (kDa)

4.98

Isoelectric Point (pI)

25.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_17	PF00332	29 - 347	6.4e-65	Glycosyl hydrolases family 17
X8	PF07983	368 - 437	8.1e-14	X8 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012549)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G31140
fragaria_vesca	FvH4_2g30580
malus_domestica	MD08G1132400.v1.1 MD15G1113000.v1.1
prunus_persica	Prupe.1G463500_v2.0.a1
pyrus_communis	pycom08g11080 pycom15g10150
rosa_chinensis	RchiOBHm_Chr6g0312431
rosa_laevigata	RLG00000010329
rosa_multiflora	Rmu_sc0008363.1_g000019
rosa_roxburghii	Rroxscaffold_7G00156800
rosa_rugosa	Rorug06G0395600 Rorug06G0395700 Rorug06G0395700
rosa_samantha	Rh6AG513100 Rh6BG523900 Rh6CG529400 Rh6DG515200
rosa_wichuraiana	Rw6G044600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	501
Acc16I	TGCGCA	1 cut(s)	855
AccI	GTMKAC	2 cut(s)	501, 1460
AccII	CGCG	1 cut(s)	1233
AciI	CCGC	1 cut(s)	1233
AclWI	GGATC	9 cut(s)	157, 170, 238, 251, 567, 1133, 1329, 1360, 1367
AcsI	RAATTY	1 cut(s)	1352
AcuI	CTGAAG	1 cut(s)	407
AcyI	GRCGYC	2 cut(s)	498, 930
AfaI	GTAC	2 cut(s)	846, 1086
AfiI	CCNNNNNNNGG	4 cut(s)	533, 756, 757, 824
AflIII	ACRYGT	1 cut(s)	322
AgsI	TTSAA	7 cut(s)	58, 151, 679, 865, 989, 1176, 1415
AhlI	ACTAGT	1 cut(s)	1190
AjnI	CCWGG	1 cut(s)	976
AloI	GAACNNNNNNTCC	2 cut(s)	385, 417
AluBI	AGCT	8 cut(s)	178, 290, 440, 662, 1036, 1164, 1282, 1403
AluI	AGCT	8 cut(s)	178, 290, 440, 662, 1036, 1164, 1282, 1403
Alw26I	GTCTC	2 cut(s)	319, 531
AlwI	GGATC	9 cut(s)	157, 170, 238, 251, 567, 1133, 1329, 1360, 1367
AlwNI	CAGNNNCTG	1 cut(s)	134
Ama87I	CYCGRG	1 cut(s)	688
AoxI	GGCC	3 cut(s)	31, 815, 919
ApeKI	GCWGC	4 cut(s)	287, 353, 602, 1073
ApoI	RAATTY	1 cut(s)	1352
Asp700I	GAANNNNTTC	1 cut(s)	1240
AspLEI	GCGC	1 cut(s)	856
AspS9I	GGNCC	2 cut(s)	829, 920
AsuHPI	GGTGA	1 cut(s)	1340
AvaI	CYCGRG	1 cut(s)	688
AvaII	GGWCC	1 cut(s)	829
BaeI	ACNNNNGTAYC	4 cut(s)	676, 709, 1013, 1046
BamHI	GGATCC	2 cut(s)	162, 243
BbvI	GCAGC	4 cut(s)	299, 340, 589, 1085
BccI	CCATC	1 cut(s)	818
BceAI	ACGGC	1 cut(s)	1105
BcgI	CGANNNNNNTGC	6 cut(s)	581, 615, 941, 975, 1362, 1396
BciT130I	CCWGG	1 cut(s)	978
BciVI	GTATCC	2 cut(s)	337, 677
BcoDI	GTCTC	2 cut(s)	319, 531
BcuI	ACTAGT	1 cut(s)	1190
BfaI	CTAG	4 cut(s)	1043, 1191, 1386, 1422
BfmI	CTRYAG	4 cut(s)	354, 718, 819, 1050
BfuI	GTATCC	2 cut(s)	337, 677
BisI	GCNGC	4 cut(s)	288, 354, 603, 1074
BlsI	GCNGC	4 cut(s)	289, 355, 604, 1075
Bme1390I	CCNGG	1 cut(s)	978
Bme18I	GGWCC	1 cut(s)	829
BmeT110I	CYCGRG	1 cut(s)	688
BmgT120I	GGNCC	2 cut(s)	829, 920
BmiI	GGNNCC	3 cut(s)	164, 245, 830
BmrFI	CCNGG	1 cut(s)	978
BmrI	ACTGGG	2 cut(s)	106, 1021
BmsI	GCATC	7 cut(s)	175, 246, 637, 724, 949, 1216, 1382
BmuI	ACTGGG	2 cut(s)	106, 1021
Bpu10I	CCTNAGC	1 cut(s)	587
Bsa29I	ATCGAT	2 cut(s)	951, 1333
BsaBI	GATNNNNATC	2 cut(s)	485, 1364
BsaHI	GRCGYC	2 cut(s)	498, 930
BsaJI	CCNNGG	3 cut(s)	555, 868, 1323
Bsc4I	CCNNNNNNNGG	4 cut(s)	533, 756, 757, 824
Bse118I	RCCGGY	2 cut(s)	452, 917
Bse1I	ACTGG	4 cut(s)	101, 433, 1016, 1345
Bse3DI	GCAATG	1 cut(s)	730
Bse8I	GATNNNNATC	2 cut(s)	485, 1364
BseBI	CCWGG	1 cut(s)	978
BseCI	ATCGAT	2 cut(s)	951, 1333
BseDI	CCNNGG	3 cut(s)	555, 868, 1323
BseGI	GGATG	1 cut(s)	1231
BseJI	GATNNNNATC	2 cut(s)	485, 1364
BseLI	CCNNNNNNNGG	4 cut(s)	533, 756, 757, 824
BseMI	GCAATG	1 cut(s)	730
BseMII	CTCAG	5 cut(s)	203, 282, 531, 578, 1051
BseNI	ACTGG	4 cut(s)	101, 433, 1016, 1345
BseRI	GAGGAG	1 cut(s)	307
BseXI	GCAGC	4 cut(s)	299, 340, 589, 1085
Bsh1236I	CGCG	1 cut(s)	1233
BshFI	GGCC	3 cut(s)	33, 817, 921
BshVI	ATCGAT	2 cut(s)	951, 1333
BsiHKCI	CYCGRG	1 cut(s)	688
BsiSI	CCGG	3 cut(s)	453, 918, 1398
BslFI	GGGAC	5 cut(s)	115, 842, 865, 916, 1028
BslI	CCNNNNNNNGG	4 cut(s)	533, 756, 757, 824
BsmAI	GTCTC	2 cut(s)	319, 531
BsmFI	GGGAC	5 cut(s)	115, 842, 865, 916, 1028
BsmI	GAATGC	4 cut(s)	211, 605, 841, 969
BsnI	GGCC	3 cut(s)	33, 817, 921
BsoBI	CYCGRG	1 cut(s)	688
Bsp1407I	TGTACA	1 cut(s)	844
Bsp143I	GATC	9 cut(s)	162, 243, 559, 807, 1138, 1330, 1334, 1359, 1365
BspACI	CCGC	1 cut(s)	1233
BspANI	GGCC	3 cut(s)	33, 817, 921
BspCNI	CTCAG	5 cut(s)	204, 283, 530, 579, 1050
BspDI	ATCGAT	2 cut(s)	951, 1333
BspFNI	CGCG	1 cut(s)	1233
BspHI	TCATGA	1 cut(s)	1362
BspLI	GGNNCC	3 cut(s)	164, 245, 830
BspMAI	CTGCAG	1 cut(s)	358
BspPI	GGATC	9 cut(s)	157, 170, 238, 251, 567, 1133, 1329, 1360, 1367
BsrDI	GCAATG	1 cut(s)	730
BsrFI	RCCGGY	2 cut(s)	452, 917
BsrGI	TGTACA	1 cut(s)	844
BsrI	ACTGG	4 cut(s)	101, 433, 1016, 1345
BssAI	RCCGGY	2 cut(s)	452, 917
BssECI	CCNNGG	3 cut(s)	555, 868, 1323
BssMI	GATC	9 cut(s)	162, 243, 559, 807, 1138, 1330, 1334, 1359, 1365
BssNAI	GTATAC	1 cut(s)	1461
BssNI	GRCGYC	2 cut(s)	498, 930
BssT1I	CCWWGG	2 cut(s)	555, 868
Bst1107I	GTATAC	1 cut(s)	1461
Bst2UI	CCWGG	1 cut(s)	978
BstACI	GRCGYC	2 cut(s)	498, 930
BstAUI	TGTACA	1 cut(s)	844
BstC8I	GCNNGC	5 cut(s)	285, 919, 1231, 1294, 1405
BstDEI	CTNAG	8 cut(s)	35, 212, 291, 517, 587, 1026, 1037, 1154
BstDSI	CCRYGG	1 cut(s)	1323
BstF5I	GGATG	1 cut(s)	1231
BstFNI	CGCG	1 cut(s)	1233
BstHHI	GCGC	1 cut(s)	856
BstKTI	GATC	9 cut(s)	165, 246, 562, 810, 1141, 1333, 1337, 1362, 1368
BstMAI	GTCTC	2 cut(s)	319, 531
BstMBI	GATC	9 cut(s)	162, 243, 559, 807, 1138, 1330, 1334, 1359, 1365
BstMWI	GCNNNNNNNGC	2 cut(s)	668, 1053
BstNI	CCWGG	1 cut(s)	978
BstNSI	RCATGY	2 cut(s)	326, 1173
BstSCI	CCNGG	1 cut(s)	976
BstSFI	CTRYAG	4 cut(s)	354, 718, 819, 1050
BstUI	CGCG	1 cut(s)	1233
BstV1I	GCAGC	4 cut(s)	299, 340, 589, 1085
BstX2I	RGATCY	3 cut(s)	162, 243, 559
BstYI	RGATCY	3 cut(s)	162, 243, 559
BstZ17I	GTATAC	1 cut(s)	1461
Bsu15I	ATCGAT	2 cut(s)	951, 1333
BsuI	GTATCC	2 cut(s)	337, 677
BsuRI	GGCC	3 cut(s)	33, 817, 921
BsuTUI	ATCGAT	2 cut(s)	951, 1333
BtgI	CCRYGG	1 cut(s)	1323
BtsCI	GGATG	1 cut(s)	1231
BtsI	GCAGTG	2 cut(s)	363, 1287
BtsIMutI	CAGTG	5 cut(s)	363, 514, 609, 1287, 1338
Cac8I	GCNNGC	5 cut(s)	285, 919, 1231, 1294, 1405
CaiI	CAGNNNCTG	1 cut(s)	134
CciI	TCATGA	1 cut(s)	1362
CfoI	GCGC	1 cut(s)	856
Cfr10I	RCCGGY	2 cut(s)	452, 917
Cfr13I	GGNCC	2 cut(s)	829, 920
ClaI	ATCGAT	2 cut(s)	951, 1333
CseI	GACGC	1 cut(s)	938
Csp6I	GTAC	2 cut(s)	845, 1085
CviAII	CATG	8 cut(s)	235, 323, 565, 743, 1114, 1170, 1212, 1363
CviQI	GTAC	2 cut(s)	845, 1085
DdeI	CTNAG	8 cut(s)	35, 212, 291, 517, 587, 1026, 1037, 1154
DpnI	GATC	9 cut(s)	164, 245, 561, 809, 1140, 1332, 1336, 1361, 1367
DpnII	GATC	9 cut(s)	162, 243, 559, 807, 1138, 1330, 1334, 1359, 1365
Eco130I	CCWWGG	2 cut(s)	555, 868
Eco147I	AGGCCT	1 cut(s)	33
Eco32I	GATATC	1 cut(s)	343
Eco47I	GGWCC	1 cut(s)	829
Eco57I	CTGAAG	1 cut(s)	407
Eco88I	CYCGRG	1 cut(s)	688
EcoO109I	RGGNCCY	1 cut(s)	829
EcoRII	CCWGG	1 cut(s)	976
EcoRV	GATATC	1 cut(s)	343
EcoT14I	CCWWGG	2 cut(s)	555, 868
EcoT22I	ATGCAT	2 cut(s)	1171, 1252
ErhI	CCWWGG	2 cut(s)	555, 868
FaeI	CATG	8 cut(s)	238, 326, 568, 746, 1117, 1173, 1215, 1366
FaqI	GGGAC	5 cut(s)	115, 842, 865, 916, 1028
FatI	CATG	8 cut(s)	234, 322, 564, 742, 1113, 1169, 1211, 1362
FblI	GTMKAC	2 cut(s)	501, 1460
Fnu4HI	GCNGC	4 cut(s)	288, 354, 603, 1074
FokI	GGATG	1 cut(s)	1238
Fsp4HI	GCNGC	4 cut(s)	288, 354, 603, 1074
FspBI	CTAG	4 cut(s)	1043, 1191, 1386, 1422
FspI	TGCGCA	1 cut(s)	855
GlaI	GCGC	1 cut(s)	855
GluI	GCNGC	4 cut(s)	288, 354, 603, 1074
HaeIII	GGCC	3 cut(s)	33, 817, 921
HapII	CCGG	3 cut(s)	453, 918, 1398
HgaI	GACGC	1 cut(s)	938
HhaI	GCGC	1 cut(s)	856
Hin1I	GRCGYC	2 cut(s)	498, 930
Hin1II	CATG	8 cut(s)	238, 326, 568, 746, 1117, 1173, 1215, 1366
Hin6I	GCGC	1 cut(s)	854
HinP1I	GCGC	1 cut(s)	854
HincII	GTYRAC	2 cut(s)	111, 381
HindII	GTYRAC	2 cut(s)	111, 381
HindIII	AAGCTT	1 cut(s)	176
HinfI	GANTC	7 cut(s)	191, 226, 481, 691, 714, 904, 1129
HpaI	GTTAAC	1 cut(s)	111
HpaII	CCGG	3 cut(s)	453, 918, 1398
HphI	GGTGA	1 cut(s)	1340
Hpy166II	GTNNAC	6 cut(s)	22, 111, 381, 502, 928, 1461
Hpy188I	TCNGA	5 cut(s)	204, 213, 426, 520, 1370
Hpy188III	TCNNGA	7 cut(s)	478, 586, 889, 1133, 1142, 1363, 1412
Hpy8I	GTNNAC	6 cut(s)	22, 111, 381, 502, 928, 1461
HpyAV	CCTTC	5 cut(s)	145, 431, 537, 637, 682
HpyCH4IV	ACGT	1 cut(s)	498
HpyF10VI	GCNNNNNNNGC	2 cut(s)	668, 1053
HpyF3I	CTNAG	8 cut(s)	35, 212, 291, 517, 587, 1026, 1037, 1154
HpySE526I	ACGT	1 cut(s)	498
Hsp92I	GRCGYC	2 cut(s)	498, 930
Hsp92II	CATG	8 cut(s)	238, 326, 568, 746, 1117, 1173, 1215, 1366
HspAI	GCGC	1 cut(s)	854
KroI	GCCGGC	1 cut(s)	917
KroNI	GCCGGC	1 cut(s)	919
KspAI	GTTAAC	1 cut(s)	111
Kzo9I	GATC	9 cut(s)	162, 243, 559, 807, 1138, 1330, 1334, 1359, 1365
LmnI	GCTCC	2 cut(s)	89, 1400
Lsp1109I	GCAGC	4 cut(s)	299, 340, 589, 1085
LweI	GCATC	7 cut(s)	175, 246, 637, 724, 949, 1216, 1382
MaeI	CTAG	4 cut(s)	1043, 1191, 1386, 1422
MaeII	ACGT	1 cut(s)	498
MaeIII	GTNAC	4 cut(s)	475, 762, 980, 1259
MalI	GATC	9 cut(s)	164, 245, 561, 809, 1140, 1332, 1336, 1361, 1367
MboI	GATC	9 cut(s)	162, 243, 559, 807, 1138, 1330, 1334, 1359, 1365
MboII	GAAGA	8 cut(s)	46, 154, 392, 667, 723, 968, 1368, 1371
MflI	RGATCY	3 cut(s)	162, 243, 559
MluCI	AATT	6 cut(s)	295, 573, 652, 795, 1352, 1464
MlyI	GAGTC	3 cut(s)	700, 898, 1138
MmeI	TCCRAC	4 cut(s)	354, 428, 549, 781
MnlI	CCTC	4 cut(s)	129, 328, 647, 956
Mph1103I	ATGCAT	2 cut(s)	1171, 1252
MroNI	GCCGGC	1 cut(s)	917
MroXI	GAANNNNTTC	1 cut(s)	1240
MseI	TTAA	4 cut(s)	110, 471, 699, 1254
MspA1I	CMGCKG	3 cut(s)	290, 662, 1282
MspI	CCGG	3 cut(s)	453, 918, 1398
MspR9I	CCNGG	1 cut(s)	978
Mva1269I	GAATGC	4 cut(s)	211, 605, 841, 969
MvaI	CCWGG	1 cut(s)	978
MvnI	CGCG	1 cut(s)	1233
MwoI	GCNNNNNNNGC	2 cut(s)	668, 1053
NaeI	GCCGGC	1 cut(s)	919
NdeII	GATC	9 cut(s)	162, 243, 559, 807, 1138, 1330, 1334, 1359, 1365
NgoMIV	GCCGGC	1 cut(s)	917
NlaIII	CATG	8 cut(s)	238, 326, 568, 746, 1117, 1173, 1215, 1366
NlaIV	GGNNCC	3 cut(s)	164, 245, 830
NmuCI	GTSAC	1 cut(s)	475
NsbI	TGCGCA	1 cut(s)	855
NsiI	ATGCAT	2 cut(s)	1171, 1252
NspI	RCATGY	2 cut(s)	326, 1173
PaeR7I	CTCGAG	1 cut(s)	688
PagI	TCATGA	1 cut(s)	1362
PceI	AGGCCT	1 cut(s)	33
PciI	ACATGT	1 cut(s)	322
PcsI	WCGNNNNNNNCGW	1 cut(s)	1124
PctI	GAATGC	4 cut(s)	211, 605, 841, 969
PdiI	GCCGGC	1 cut(s)	919
PdmI	GAANNNNTTC	1 cut(s)	1240
PfeI	GAWTC	4 cut(s)	191, 226, 481, 714
PkrI	GCNGC	4 cut(s)	289, 355, 604, 1075
PleI	GAGTC	3 cut(s)	699, 898, 1137
PpsI	GAGTC	3 cut(s)	699, 898, 1137
PpuMI	RGGWCCY	1 cut(s)	829
PscI	ACATGT	1 cut(s)	322
Psp5II	RGGWCCY	1 cut(s)	829
Psp6I	CCWGG	1 cut(s)	976
PspGI	CCWGG	1 cut(s)	976
PspN4I	GGNNCC	3 cut(s)	164, 245, 830
PspPI	GGNCC	2 cut(s)	829, 920
PspPPI	RGGWCCY	1 cut(s)	829
PspXI	VCTCGAGB	1 cut(s)	688
PstI	CTGCAG	1 cut(s)	358
PstNI	CAGNNNCTG	1 cut(s)	134
PsuI	RGATCY	3 cut(s)	162, 243, 559
PvuII	CAGCTG	3 cut(s)	290, 662, 1282
RsaI	GTAC	2 cut(s)	846, 1086
RsaNI	GTAC	2 cut(s)	845, 1085
SaqAI	TTAA	4 cut(s)	110, 471, 699, 1254
SatI	GCNGC	4 cut(s)	288, 354, 603, 1074
Sau3AI	GATC	9 cut(s)	162, 243, 559, 807, 1138, 1330, 1334, 1359, 1365
Sau96I	GGNCC	2 cut(s)	829, 920
SchI	GAGTC	3 cut(s)	700, 898, 1138
ScrFI	CCNGG	1 cut(s)	978
SfaNI	GCATC	7 cut(s)	175, 246, 637, 724, 949, 1216, 1382
SfcI	CTRYAG	4 cut(s)	354, 718, 819, 1050
Sfr274I	CTCGAG	1 cut(s)	688
SinI	GGWCC	1 cut(s)	829
SlaI	CTCGAG	1 cut(s)	688
SmlI	CTYRAG	1 cut(s)	688
SmoI	CTYRAG	1 cut(s)	688
SpeI	ACTAGT	1 cut(s)	1190
Sse9I	AATT	6 cut(s)	295, 573, 652, 795, 1352, 1464
SseBI	AGGCCT	1 cut(s)	33
SsiI	CCGC	1 cut(s)	1233
SspI	AATATT	1 cut(s)	411
SspMI	CTAG	4 cut(s)	1043, 1191, 1386, 1422
StuI	AGGCCT	1 cut(s)	33
StyD4I	CCNGG	1 cut(s)	976
StyI	CCWWGG	2 cut(s)	555, 868
TaiI	ACGT	1 cut(s)	501
TaqI	TCGA	7 cut(s)	183, 544, 689, 951, 1058, 1313, 1333
TasI	AATT	6 cut(s)	295, 573, 652, 795, 1352, 1464
TatI	WGTACW	1 cut(s)	844
TfiI	GAWTC	4 cut(s)	191, 226, 481, 714
Tru1I	TTAA	4 cut(s)	110, 471, 699, 1254
Tru9I	TTAA	4 cut(s)	110, 471, 699, 1254
TscAI	CASTG	5 cut(s)	363, 514, 616, 1294, 1345
TseFI	GTSAC	1 cut(s)	475
TseI	GCWGC	4 cut(s)	287, 353, 602, 1073
Tsp45I	GTSAC	1 cut(s)	475
TspDTI	ATGAA	4 cut(s)	381, 619, 969, 1200
TspGWI	ACGGA	1 cut(s)	1032
TspRI	CASTG	5 cut(s)	363, 514, 616, 1294, 1345
VpaK11BI	GGWCC	1 cut(s)	829
XapI	RAATTY	1 cut(s)	1352
XceI	RCATGY	2 cut(s)	326, 1173
XhoI	CTCGAG	1 cut(s)	688
XmiI	GTMKAC	2 cut(s)	501, 1460
XmnI	GAANNNNTTC	1 cut(s)	1240
XspI	CTAG	4 cut(s)	1043, 1191, 1386, 1422
ZraI	GACGTC	1 cut(s)	499
Zsp2I	ATGCAT	2 cut(s)	1171, 1252

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.