pycom15g10680

Carbon catabolite repressor protein 4 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0005330
Physical Location & Seq
Forward (+)
7149971 .. 7154953
4983 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2412 bp
ATGAGACACGCTCCTCCTCCTTCCCTCCAATTCCTCGCCGCTGCCGCCACCAACTCCGCCGTCACCACCATGTCTTCGCGCCCTCCTTACCGAGGTGGTCGAAACCCATGGCAGAGAGGCTTCTCCAACCGCCCCTACTCAGGCGGCGGTAGGAACAACTACGTCACCGGAGACTCCCACTTCCGCTCAGTCCGCGACGCCAATCTAGGGTCCCGGCAAGGCGATTCCGGACCCTTCCCCAACCAAACCTCCTATCGTCGGACTCCGCCGCCGTCTTTCGATGTCAACCACTTCCGCGGTCCTCCGCAGCTCGATCACAATCAACCGTACCGGCCGAATCAGCAATTTCGGCCTCCCCATCAGTTACGCCCCCCTCAGCAGTTTCGGCCGCCGCAGCAATTCCGGCCTCGGCCGAAACCCTTGGACTATCGGAACTGGGAATTTGCCAAAACGACACCGTCCCCTACTTGTGAGAGGTTTACTGTGTTGTCATATAACATACTAGCCGATTACTTGGCTAACGATCACCGGAGTAAGCTATACTATCACATACCTCATCATATGATGGACTGGCTGTGGAGAAAGCGAAATTTGATATTCGAGCTCGGGCTGTGGTCACCCGACATAATGTGCCTTCAGGAAGTTGATAAATTTGAGGACCTTGAGGAGGAGTTGAAGCTTAAAGGGTACATCGGCATTTGGAAGATGAGGACTGGCAATCCAGTTGATGGATGTGCAATTTTTTGGAGATCGTCAAGATTCAAGTTGGTCCATGAGGAATCCATCGAGTTCAATAAGCTTGGACTTCGGGATAATGTTGCTCAAATATGCGTGCTGGAGTCAATACGTCAGAATAGCACCAAAAATATTGCAGCTCTACCAACAAGCTCAACAGGTACTAATAAAGTTGTCATTTGTAACATTCATGTGCTTTACAACCCTAGACGGGGAGAAATTAAGCTTGGCCAGGTCAGGGTTCTTTTGGATAGGGCTCATGCTGTTTCCAAAATCTGGAATGATGCTCCAATTGTTCTTTGTGGGGATTTCAATTGTACACCTAAGAGTCCGTTATACAACTTTATTTCAGAACAGAAGTTAGATTTGTCTGGAGTTGACAGAGATAAGGTATCTGGACAAGCTTCAGCTCAAATTTATGCACCAAGGTCATATGATCATAATTATGGGGTTGGACCTGCTAGTCATATAGCTCAAGGTCCACCAATTGTAGATGGGAGGGAAGCTGGATTGAACTCGGGCAATCTGTCATTGGATATCCAGAACCCAAATAATCCAGAAAGTAGTGCAGAGAGTGTTCCATTAGCGAACAACTTGTCTCAGCCTCAAAGTACTGACACTCCAGACGTTTTTGATAAGTCTTGCCCCAATGTGCATTTTGGAAAAGATGACGGTTCCAAAGATGTTGATGTGAACCAAGAAACTAAGCAGAACTCTTTAGATGTCTCTGAGGTTGAAACTGGATCAGCATGCCATGTGACTGAGATGGGATACGAAGAAAAAGTGGATTTCACCTCTAATTCAAATCATGGATTATCGAGCGGACATTTGAATTCAGATGTTTGCCTAGAGAAAGAAAGGTTGAACTGTGATATCCATAAACATCTCTCTACGAGGGACAATGATTCTATTAGGGAAGGGACTGATCCAGATTCTGTGGTTCCTATCGACTCAGAAGTTCCTTTGTCTAAGCTATCTAACCAGGCTTCCATTACCGATGATACCAAAGTCTCTTCATTGGAAAACTTAGGGCATTTGTCTTCTGATCCAGTTTCTGGTGTCGTGGGGGAAAGCCCTTCAGACTCACATCAAGTGGATATATCATGTCCATCTGCTAGTGCTGACCTTGAATTGCAGAAAAATATGGAAAGTTTATCTCTTAATGTGCTAGATGAAGCCATTCCGGCAGATGAAAATACAGCTGAAGATGATAAAACGTTTATATCTGCATTACACGATATGGAAGATGCCTTTCCATCTGATTTTACTTCAGTTACTCCCAACTCAGCATTTTTGGCATCACCCAATGCAGAGAGAACCTATTATGATCCATCACTATGGACTCCGATGGAAATAGAAACTGCCACAGGCAATGCGGAACACACTCTTTTGGAACATCCTCTGAAGCTTAAGAGCACATATACTGAAGTTGAGGATTGTTCTGGCACCAGAGATTCAAGTGGGGAGCCCCAGGTGACCAGTTATAATAAATTATTCTTGGGCACGGTTGATTACATCTGGCGTTCCGAAGGTCTGCAGACTGTCAGGGTGCTTGCTCCTATTCCAAAGCAAGCTATGCAGTGGACTTCAGGGTTCCCAACCAAGAAATGGGGTAGTGATCATATTGCATTGGCTTCTGAGTTGTCATTCATCAACAATTCTGACCAATCATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo_endo_phos

Protein Analysis

804

Amino Acids

89.44

Weight (kDa)

5.47

Isoelectric Point (pI)

46.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 205 - 394 9.6e-12 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2220
AasI GACNNNNNNGTC 1 cut(s) 1197
Acc36I ACCTGC 1 cut(s) 1201
AccB1I GGYRCC 1 cut(s) 2180
AccB7I CCANNNNNTGG 4 cut(s) 728, 1011, 1790, 2343
AccBSI CCGCTC 2 cut(s) 186, 1557
AccII CGCG 3 cut(s) 79, 195, 297
AccIII TCCGGA 1 cut(s) 227
AclI AACGTT 1 cut(s) 1952
AclWI GGATC 4 cut(s) 1486, 1655, 1775, 2057
AcoI YGGCCR 4 cut(s) 332, 386, 410, 964
AcsI RAATTY 5 cut(s) 440, 589, 650, 1149, 1567
AcuI CTGAAG 8 cut(s) 620, 1125, 1797, 1959, 1989, 2159, 2181, 2307
AcyI GRCGYC 1 cut(s) 198
AfaI GTAC 5 cut(s) 329, 689, 898, 1054, 1348
AflII CTTAAG 1 cut(s) 2144
AjnI CCWGG 3 cut(s) 966, 1716, 2205
AjuI GAANNNNNNNTTGG 2 cut(s) 945, 977
Alw21I GWGCWC 2 cut(s) 606, 2153
Alw26I GTCTC 4 cut(s) 165, 1338, 1465, 1750
AlwI GGATC 4 cut(s) 1486, 1655, 1775, 2057
AlwNI CAGNNNCTG 2 cut(s) 1670, 1790
Ama87I CYCGRG 2 cut(s) 605, 1252
Aor13HI TCCGGA 1 cut(s) 227
AoxI GGCC 6 cut(s) 332, 350, 386, 404, 410, 964
ApeKI GCWGC 4 cut(s) 41, 307, 394, 872
ApoI RAATTY 5 cut(s) 440, 589, 650, 1149, 1567
ArsI GACNNNNNNTTYG 2 cut(s) 1316, 1348
Asp700I GAANNNNTTC 1 cut(s) 1914
AspLEI GCGC 1 cut(s) 81
AspS9I GGNCC 7 cut(s) 210, 230, 299, 658, 769, 1190, 1214
AsuC2I CCSGG 1 cut(s) 214
AsuHPI GGTGA 7 cut(s) 55, 157, 518, 609, 1519, 2028, 2221
AvaI CYCGRG 2 cut(s) 605, 1252
AvaII GGWCC 7 cut(s) 210, 230, 299, 658, 769, 1190, 1214
BaeGI GKGCMC 1 cut(s) 2240
BaeI ACNNNNGTAYC 2 cut(s) 1728, 1761
BalI TGGCCA 1 cut(s) 966
BanI GGYRCC 1 cut(s) 2180
BanII GRGCYC 3 cut(s) 606, 994, 2205
BbsI GAAGAC 2 cut(s) 66, 1767
Bbv12I GWGCWC 2 cut(s) 606, 2153
BbvCI CCTCAGC 1 cut(s) 375
BbvI GCAGC 4 cut(s) 28, 319, 406, 884
BceAI ACGGC 2 cut(s) 44, 256
BciT130I CCWGG 3 cut(s) 968, 1718, 2207
BciVI GTATCC 1 cut(s) 1499
BclI TGATCA 2 cut(s) 1171, 2353
BcnI CCSGG 1 cut(s) 214
BcoDI GTCTC 4 cut(s) 165, 1338, 1465, 1750
BfaI CTAG 7 cut(s) 206, 503, 942, 1197, 1583, 1851, 1904
BfmI CTRYAG 1 cut(s) 2270
BfrI CTTAAG 1 cut(s) 2144
BfuAI ACCTGC 1 cut(s) 1201
BfuI GTATCC 1 cut(s) 1499
BglI GCCNNNNNGGC 1 cut(s) 1919
BmcAI AGTACT 1 cut(s) 1348
Bme1390I CCNGG 4 cut(s) 214, 968, 1718, 2207
Bme18I GGWCC 7 cut(s) 210, 230, 299, 658, 769, 1190, 1214
BmeT110I CYCGRG 2 cut(s) 605, 1252
BmgT120I GGNCC 7 cut(s) 210, 230, 299, 658, 769, 1190, 1214
BmiI GGNNCC 8 cut(s) 211, 212, 232, 1411, 1677, 2182, 2202, 2330
BmrFI CCNGG 4 cut(s) 214, 968, 1718, 2207
BmrI ACTGGG 1 cut(s) 445
BmsI GCATC 3 cut(s) 1009, 1972, 2042
BmuI ACTGGG 1 cut(s) 445
BpiI GAAGAC 2 cut(s) 66, 1767
BplI GAGNNNNNCTC 1 cut(s) 27
BpmI CTGGAG 3 cut(s) 857, 1128, 1341
Bpu10I CCTNAGC 1 cut(s) 375
BpuEI CTTGAG 2 cut(s) 683, 1194
BpuMI CCSGG 1 cut(s) 214
BsaBI GATNNNNATC 1 cut(s) 318
BsaHI GRCGYC 1 cut(s) 198
BsaJI CCNNGG 7 cut(s) 91, 107, 295, 407, 420, 1160, 2205
BsaWI WCCGGW 3 cut(s) 167, 227, 528
BsaXI ACNNNNNCTCC 4 cut(s) 233, 263, 1339, 1369
Bse118I RCCGGY 1 cut(s) 330
Bse1I ACTGG 7 cut(s) 440, 575, 718, 722, 1480, 1784, 2214
Bse3DI GCAATG 1 cut(s) 2113
Bse8I GATNNNNATC 1 cut(s) 318
BseAI TCCGGA 1 cut(s) 227
BseBI CCWGG 3 cut(s) 968, 1718, 2207
BseDI CCNNGG 7 cut(s) 91, 107, 295, 407, 420, 1160, 2205
BseGI GGATG 2 cut(s) 737, 2131
BseJI GATNNNNATC 1 cut(s) 318
BseMI GCAATG 1 cut(s) 2113
BseMII CTCAG 9 cut(s) 153, 201, 389, 1349, 1455, 1488, 1701, 2034, 2364
BseNI ACTGG 7 cut(s) 440, 575, 718, 722, 1480, 1784, 2214
BseRI GAGGAG 3 cut(s) 6, 680, 683
BseSI GKGCMC 1 cut(s) 2240
BseX3I CGGCCG 3 cut(s) 332, 386, 410
BseXI GCAGC 4 cut(s) 28, 319, 406, 884
BsgI GTGCAG 1 cut(s) 1323
Bsh1236I CGCG 3 cut(s) 79, 195, 297
Bsh1285I CGRYCG 3 cut(s) 335, 389, 413
BshFI GGCC 6 cut(s) 334, 352, 388, 406, 412, 966
BshNI GGYRCC 1 cut(s) 2180
BsiEI CGRYCG 3 cut(s) 335, 389, 413
BsiHKAI GWGCWC 2 cut(s) 606, 2153
BsiHKCI CYCGRG 2 cut(s) 605, 1252
BsiSI CCGG 7 cut(s) 168, 214, 228, 331, 403, 529, 1919
BslFI GGGAC 4 cut(s) 196, 445, 1646, 1669
BsmAI GTCTC 4 cut(s) 165, 1338, 1465, 1750
BsmFI GGGAC 4 cut(s) 196, 445, 1646, 1669
BsnI GGCC 6 cut(s) 334, 352, 388, 406, 412, 966
BsoBI CYCGRG 2 cut(s) 605, 1252
Bsp1286I GDGCHC 5 cut(s) 606, 994, 2153, 2205, 2240
Bsp13I TCCGGA 1 cut(s) 227
Bsp1407I TGTACA 1 cut(s) 1052
Bsp143I GATC 9 cut(s) 313, 523, 749, 1171, 1478, 1660, 1780, 2062, 2353
Bsp19I CCATGG 1 cut(s) 107
BspANI GGCC 6 cut(s) 334, 352, 388, 406, 412, 966
BspCNI CTCAG 9 cut(s) 152, 200, 388, 1348, 1456, 1489, 1700, 2033, 2365
BspEI TCCGGA 1 cut(s) 227
BspFNI CGCG 3 cut(s) 79, 195, 297
BspLI GGNNCC 8 cut(s) 211, 212, 232, 1411, 1677, 2182, 2202, 2330
BspMAI CTGCAG 1 cut(s) 2274
BspMI ACCTGC 1 cut(s) 1201
BspPI GGATC 4 cut(s) 1486, 1655, 1775, 2057
BspT107I GGYRCC 1 cut(s) 2180
BspTI CTTAAG 1 cut(s) 2144
BsrBI CCGCTC 2 cut(s) 186, 1557
BsrDI GCAATG 1 cut(s) 2113
BsrFI RCCGGY 1 cut(s) 330
BsrGI TGTACA 1 cut(s) 1052
BsrI ACTGG 7 cut(s) 440, 575, 718, 722, 1480, 1784, 2214
BssAI RCCGGY 1 cut(s) 330
BssECI CCNNGG 7 cut(s) 91, 107, 295, 407, 420, 1160, 2205
BssMI GATC 9 cut(s) 313, 523, 749, 1171, 1478, 1660, 1780, 2062, 2353
BssNI GRCGYC 1 cut(s) 198
BssT1I CCWWGG 3 cut(s) 107, 420, 1160
Bst2UI CCWGG 3 cut(s) 968, 1718, 2207
Bst4CI ACNGT 7 cut(s) 327, 459, 484, 1409, 1604, 2242, 2278
Bst6I CTCTTC 1 cut(s) 1753
BstACI GRCGYC 1 cut(s) 198
BstAFI CTTAAG 1 cut(s) 2144
BstAPI GCANNNNNTGC 1 cut(s) 2311
BstAUI TGTACA 1 cut(s) 1052
BstC8I GCNNGC 4 cut(s) 833, 1486, 2289, 2307
BstDSI CCRYGG 2 cut(s) 107, 295
BstEII GGTNACC 2 cut(s) 615, 2209
BstENI CCTNNNNNAGG 2 cut(s) 90, 665
BstF5I GGATG 2 cut(s) 737, 2131
BstFNI CGCG 3 cut(s) 79, 195, 297
BstHHI GCGC 1 cut(s) 81
BstKTI GATC 9 cut(s) 316, 526, 752, 1174, 1481, 1663, 1783, 2065, 2356
BstMAI GTCTC 4 cut(s) 165, 1338, 1465, 1750
BstMBI GATC 9 cut(s) 313, 523, 749, 1171, 1478, 1660, 1780, 2062, 2353
BstMCI CGRYCG 3 cut(s) 335, 389, 413
BstNI CCWGG 3 cut(s) 968, 1718, 2207
BstNSI RCATGY 1 cut(s) 1488
BstPI GGTNACC 2 cut(s) 615, 2209
BstSCI CCNGG 4 cut(s) 212, 966, 1716, 2205
BstSFI CTRYAG 1 cut(s) 2270
BstSLI GKGCMC 1 cut(s) 2240
BstUI CGCG 3 cut(s) 79, 195, 297
BstV1I GCAGC 4 cut(s) 28, 319, 406, 884
BstV2I GAAGAC 2 cut(s) 66, 1767
BstXI CCANNNNNNTGG 1 cut(s) 2073
BstZI CGGCCG 3 cut(s) 332, 386, 410
BsuI GTATCC 1 cut(s) 1499
BsuRI GGCC 6 cut(s) 334, 352, 388, 406, 412, 966
BtgI CCRYGG 2 cut(s) 107, 295
BtsCI GGATG 2 cut(s) 737, 2131
BtsI GCAGTG 1 cut(s) 2321
BtsIMutI CAGTG 1 cut(s) 2321
BveI ACCTGC 1 cut(s) 1201
Cac8I GCNNGC 4 cut(s) 833, 1486, 2289, 2307
CaiI CAGNNNCTG 2 cut(s) 1670, 1790
CfoI GCGC 1 cut(s) 81
Cfr10I RCCGGY 1 cut(s) 330
Cfr13I GGNCC 7 cut(s) 210, 230, 299, 658, 769, 1190, 1214
Cfr42I CCGCGG 1 cut(s) 298
CseI GACGC 1 cut(s) 206
Csp6I GTAC 5 cut(s) 328, 688, 897, 1053, 1347
CviAII CATG 9 cut(s) 70, 108, 773, 926, 995, 1485, 1490, 1544, 1839
CviQI GTAC 5 cut(s) 328, 688, 897, 1053, 1347
DpnI GATC 9 cut(s) 315, 525, 751, 1173, 1480, 1662, 1782, 2064, 2355
DpnII GATC 9 cut(s) 313, 523, 749, 1171, 1478, 1660, 1780, 2062, 2353
DrdI GACNNNNNNGTC 1 cut(s) 1197
DseDI GACNNNNNNGTC 1 cut(s) 1197
EaeI YGGCCR 4 cut(s) 332, 386, 410, 964
EagI CGGCCG 3 cut(s) 332, 386, 410
Eam1104I CTCTTC 1 cut(s) 1753
EarI CTCTTC 1 cut(s) 1753
EciI GGCGGA 2 cut(s) 46, 255
Ecl136II GAGCTC 1 cut(s) 604
EclXI CGGCCG 3 cut(s) 332, 386, 410
Eco130I CCWWGG 3 cut(s) 107, 420, 1160
Eco24I GRGCYC 3 cut(s) 606, 994, 2205
Eco32I GATATC 2 cut(s) 1273, 1609
Eco47I GGWCC 7 cut(s) 210, 230, 299, 658, 769, 1190, 1214
Eco52I CGGCCG 3 cut(s) 332, 386, 410
Eco53kI GAGCTC 1 cut(s) 604
Eco57I CTGAAG 8 cut(s) 620, 1125, 1797, 1959, 1989, 2159, 2181, 2307
Eco88I CYCGRG 2 cut(s) 605, 1252
Eco91I GGTNACC 2 cut(s) 615, 2209
EcoICRI GAGCTC 1 cut(s) 604
EcoNI CCTNNNNNAGG 2 cut(s) 90, 665
EcoO109I RGGNCCY 2 cut(s) 210, 658
EcoO65I GGTNACC 2 cut(s) 615, 2209
EcoRI GAATTC 1 cut(s) 1567
EcoRII CCWGG 3 cut(s) 966, 1716, 2205
EcoRV GATATC 2 cut(s) 1273, 1609
EcoT14I CCWWGG 3 cut(s) 107, 420, 1160
EcoT38I GRGCYC 3 cut(s) 606, 994, 2205
ErhI CCWWGG 3 cut(s) 107, 420, 1160
FaeI CATG 9 cut(s) 73, 111, 776, 929, 998, 1488, 1493, 1547, 1842
FaqI GGGAC 4 cut(s) 196, 445, 1646, 1669
FatI CATG 9 cut(s) 69, 107, 772, 925, 994, 1484, 1489, 1543, 1838
FauNDI CATATG 2 cut(s) 561, 1168
FbaI TGATCA 2 cut(s) 1171, 2353
FokI GGATG 2 cut(s) 744, 2118
FriOI GRGCYC 3 cut(s) 606, 994, 2205
FspBI CTAG 7 cut(s) 206, 503, 942, 1197, 1583, 1851, 1904
GlaI GCGC 1 cut(s) 80
GsuI CTGGAG 3 cut(s) 857, 1128, 1341
HaeIII GGCC 6 cut(s) 334, 352, 388, 406, 412, 966
HapII CCGG 7 cut(s) 168, 214, 228, 331, 403, 529, 1919
HgaI GACGC 1 cut(s) 206
HhaI GCGC 1 cut(s) 81
Hin1I GRCGYC 1 cut(s) 198
Hin1II CATG 9 cut(s) 73, 111, 776, 929, 998, 1488, 1493, 1547, 1842
Hin6I GCGC 1 cut(s) 79
HinP1I GCGC 1 cut(s) 79
HincII GTYRAC 2 cut(s) 286, 1114
HindII GTYRAC 2 cut(s) 286, 1114
HindIII AAGCTT 5 cut(s) 677, 797, 959, 1137, 2141
HpaII CCGG 7 cut(s) 168, 214, 228, 331, 403, 529, 1919
HphI GGTGA 7 cut(s) 55, 157, 518, 609, 1519, 2028, 2221
Hpy166II GTNNAC 7 cut(s) 286, 480, 1055, 1114, 1217, 1429, 2319
Hpy8I GTNNAC 7 cut(s) 286, 480, 1055, 1114, 1217, 1429, 2319
Hpy99I CGWCG 2 cut(s) 200, 261
HpyAV CCTTC 6 cut(s) 30, 244, 644, 1646, 1821, 2258
HpyCH4III ACNGT 7 cut(s) 327, 459, 484, 1409, 1604, 2242, 2278
HpyCH4IV ACGT 4 cut(s) 162, 847, 1362, 1952
HpySE526I ACGT 4 cut(s) 162, 847, 1362, 1952
Hsp92I GRCGYC 1 cut(s) 198
Hsp92II CATG 9 cut(s) 73, 111, 776, 929, 998, 1488, 1493, 1547, 1842
HspAI GCGC 1 cut(s) 79
KflI GGGWCCC 1 cut(s) 210
Kpn2I TCCGGA 1 cut(s) 227
Ksp22I TGATCA 2 cut(s) 1171, 2353
KspI CCGCGG 1 cut(s) 298
Kzo9I GATC 9 cut(s) 313, 523, 749, 1171, 1478, 1660, 1780, 2062, 2353
LmnI GCTCC 4 cut(s) 16, 1027, 2200, 2296
Lsp1109I GCAGC 4 cut(s) 28, 319, 406, 884
LweI GCATC 3 cut(s) 1009, 1972, 2042
MaeI CTAG 7 cut(s) 206, 503, 942, 1197, 1583, 1851, 1904
MaeII ACGT 4 cut(s) 162, 847, 1362, 1952
MaeIII GTNAC 8 cut(s) 61, 163, 363, 615, 917, 1492, 2008, 2209
MalI GATC 9 cut(s) 315, 525, 751, 1173, 1480, 1662, 1782, 2064, 2355
MbiI CCGCTC 2 cut(s) 186, 1557
MboI GATC 9 cut(s) 313, 523, 749, 1171, 1478, 1660, 1780, 2062, 2353
MboII GAAGA 7 cut(s) 66, 715, 1523, 1740, 1767, 1952, 1991
MfeI CAATTG 3 cut(s) 1026, 1048, 1221
MhlI GDGCHC 5 cut(s) 606, 994, 2153, 2205, 2240
MlsI TGGCCA 1 cut(s) 966
MluNI TGGCCA 1 cut(s) 966
MlyI GAGTC 7 cut(s) 167, 256, 848, 1072, 1679, 1811, 2071
MmeI TCCRAC 3 cut(s) 150, 239, 1168
Mox20I TGGCCA 1 cut(s) 966
MroI TCCGGA 1 cut(s) 227
MroXI GAANNNNTTC 1 cut(s) 1914
MscI TGGCCA 1 cut(s) 966
MseI TTAA 4 cut(s) 681, 957, 1896, 2145
MslI CAYNNNNRTG 4 cut(s) 68, 926, 1179, 2071
Msp20I TGGCCA 1 cut(s) 966
MspA1I CMGCKG 3 cut(s) 41, 297, 1937
MspCI CTTAAG 1 cut(s) 2144
MspI CCGG 7 cut(s) 168, 214, 228, 331, 403, 529, 1919
MspR9I CCNGG 4 cut(s) 214, 968, 1718, 2207
MunI CAATTG 3 cut(s) 1026, 1048, 1221
MvaI CCWGG 3 cut(s) 968, 1718, 2207
MvnI CGCG 3 cut(s) 79, 195, 297
NciI CCSGG 1 cut(s) 214
NcoI CCATGG 1 cut(s) 107
NdeI CATATG 2 cut(s) 561, 1168
NdeII GATC 9 cut(s) 313, 523, 749, 1171, 1478, 1660, 1780, 2062, 2353
NlaIII CATG 9 cut(s) 73, 111, 776, 929, 998, 1488, 1493, 1547, 1842
NlaIV GGNNCC 8 cut(s) 211, 212, 232, 1411, 1677, 2182, 2202, 2330
NmeAIII GCCGAG 1 cut(s) 388
NmuCI GTSAC 5 cut(s) 61, 163, 615, 1492, 2209
NspI RCATGY 1 cut(s) 1488
PaeI GCATGC 1 cut(s) 1488
PdmI GAANNNNTTC 1 cut(s) 1914
PfeI GAWTC 7 cut(s) 224, 337, 759, 779, 1640, 1667, 2189
PflFI GACNNNGTC 1 cut(s) 457
PflMI CCANNNNNTGG 4 cut(s) 728, 1011, 1790, 2343
PleI GAGTC 7 cut(s) 167, 256, 847, 1071, 1679, 1811, 2071
PpsI GAGTC 7 cut(s) 167, 256, 847, 1071, 1679, 1811, 2071
PpuMI RGGWCCY 2 cut(s) 210, 658
PsiI TTATAA 1 cut(s) 2220
Psp124BI GAGCTC 1 cut(s) 606
Psp1406I AACGTT 1 cut(s) 1952
Psp5II RGGWCCY 2 cut(s) 210, 658
Psp6I CCWGG 3 cut(s) 966, 1716, 2205
PspEI GGTNACC 2 cut(s) 615, 2209
PspGI CCWGG 3 cut(s) 966, 1716, 2205
PspN4I GGNNCC 8 cut(s) 211, 212, 232, 1411, 1677, 2182, 2202, 2330
PspPI GGNCC 7 cut(s) 210, 230, 299, 658, 769, 1190, 1214
PspPPI RGGWCCY 2 cut(s) 210, 658
PstI CTGCAG 1 cut(s) 2274
PstNI CAGNNNCTG 2 cut(s) 1670, 1790
PsyI GACNNNGTC 1 cut(s) 457
PvuII CAGCTG 1 cut(s) 1937
RsaI GTAC 5 cut(s) 329, 689, 898, 1054, 1348
RsaNI GTAC 5 cut(s) 328, 688, 897, 1053, 1347
RseI CAYNNNNRTG 4 cut(s) 68, 926, 1179, 2071
SacI GAGCTC 1 cut(s) 606
SacII CCGCGG 1 cut(s) 298
SaqAI TTAA 4 cut(s) 681, 957, 1896, 2145
Sau3AI GATC 9 cut(s) 313, 523, 749, 1171, 1478, 1660, 1780, 2062, 2353
Sau96I GGNCC 7 cut(s) 210, 230, 299, 658, 769, 1190, 1214
ScaI AGTACT 1 cut(s) 1348
SchI GAGTC 7 cut(s) 167, 256, 848, 1072, 1679, 1811, 2071
ScrFI CCNGG 4 cut(s) 214, 968, 1718, 2207
SduI GDGCHC 5 cut(s) 606, 994, 2153, 2205, 2240
SfaNI GCATC 3 cut(s) 1009, 1972, 2042
SfcI CTRYAG 1 cut(s) 2270
Sfr303I CCGCGG 1 cut(s) 298
SgrBI CCGCGG 1 cut(s) 298
SinI GGWCC 7 cut(s) 210, 230, 299, 658, 769, 1190, 1214
SmiMI CAYNNNNRTG 4 cut(s) 68, 926, 1179, 2071
SmlI CTYRAG 3 cut(s) 662, 1209, 2144
SmoI CTYRAG 3 cut(s) 662, 1209, 2144
SphI GCATGC 1 cut(s) 1488
SspI AATATT 1 cut(s) 868
SspMI CTAG 7 cut(s) 206, 503, 942, 1197, 1583, 1851, 1904
SstI GAGCTC 1 cut(s) 606
StyD4I CCNGG 4 cut(s) 212, 966, 1716, 2205
StyI CCWWGG 3 cut(s) 107, 420, 1160
TaaI ACNGT 7 cut(s) 327, 459, 484, 1409, 1604, 2242, 2278
TaiI ACGT 4 cut(s) 165, 850, 1365, 1955
TaqI TCGA 7 cut(s) 100, 279, 312, 600, 786, 1553, 1683
TatI WGTACW 2 cut(s) 1052, 1346
TauI GCSGC 6 cut(s) 41, 47, 147, 271, 391, 394
TfiI GAWTC 7 cut(s) 224, 337, 759, 779, 1640, 1667, 2189
Tru1I TTAA 4 cut(s) 681, 957, 1896, 2145
Tru9I TTAA 4 cut(s) 681, 957, 1896, 2145
TscAI CASTG 1 cut(s) 2321
TseFI GTSAC 5 cut(s) 61, 163, 615, 1492, 2209
TseI GCWGC 4 cut(s) 41, 307, 394, 872
Tsp45I GTSAC 5 cut(s) 61, 163, 615, 1492, 2209
TspDTI ATGAA 5 cut(s) 914, 1740, 1923, 1941, 2374
TspGWI ACGGA 1 cut(s) 1056
TspRI CASTG 1 cut(s) 2321
Tth111I GACNNNGTC 1 cut(s) 457
Van91I CCANNNNNTGG 4 cut(s) 728, 1011, 1790, 2343
Vha464I CTTAAG 1 cut(s) 2144
VpaK11BI GGWCC 7 cut(s) 210, 230, 299, 658, 769, 1190, 1214
XagI CCTNNNNNAGG 2 cut(s) 90, 665
XapI RAATTY 5 cut(s) 440, 589, 650, 1149, 1567
XceI RCATGY 1 cut(s) 1488
XcmI CCANNNNNNNNNTGG 1 cut(s) 2340
XmnI GAANNNNTTC 1 cut(s) 1914
XspI CTAG 7 cut(s) 206, 503, 942, 1197, 1583, 1851, 1904
ZrmI AGTACT 1 cut(s) 1348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.