pycom15g11310

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009226
Physical Location & Seq
Forward (+)
7645436 .. 7647662
2227 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1215 bp
ATGAAGTGGTCGCCGTGGCCGTCAGGCGGAACAAAGAGGTTTCAAGTGAAGGTGAGCCAGCTGAAGCTGCAAGGGTTTAATTTTGAGAAGGAGAAGGATAGTGTTGGTGTGATTGAGGTGAAATGGAAAGGGTCTAAGCCTGGAGGGCCTGGAGTTTTGGTTCCATTCTATGGAAGAAGATCGTCGAGGCAGCCGAAAAACTACACCAGCCAGAGATTTTTGAGTCGTCAAGTCATTGAATGGGATGATGAATTTCAGAGCCTCTGTAATTTTGGGTCGAAGCAAGGTGGGAACTTTAGTCCCTGGGATTTGACTTTTACCCTGTTACACGGAGAGAGTGCGGAGCAAAAGAGTAATATGGCGGTTTTTGGGACGGTTTCGTTGAACCTAGCAGAATTGGCATCGACAATGGAGTCTCAGATTCAGAGGAAGCTCTCTGTTACTTTGAAAATGGAGGGGACGACTCCCAGCGAAGCTACCCTTTTGGTTTGTCTAAGCTTTGCTGAGGTGAGAAACTTGCATGACTCGGCAGCACTCGGTCAAGACTCGAACGAGTCAGACAAGGGCAAGTTGGTTCGAAGAGTAAGCTACTTCAGGAGCTTTAGGAAGTCAAGCAATGAGAAGAAGAGTAGTGAGGACCGAGGCATGAGTGACCGGGACGAGTCATTCTTGTCCGACTCAGAAGGGTCACCGGGGAATGGACTCGTTAACAGTGGTGTCAATGCAGATTTGAGTTCAAAGGGAGAGTCAGGATCGGTCCTGAGTCATGAGACTCAGTTTGACTCGGGGCAGAAGAGTTGGCTCTCGAGGAAGAGGAAGTGGTTGAATGGAAGACCACCTAGGAGAAAAGTGGAGCCCTTTGTTGAGAAGACTAGTAGGGACAATGCAGTGCAAATTTCCGTTGACAACAATCTCAGTGATTCTATAAAGATATCAAATTGTACTGCTGAACGTTCTTCACAATCAGATCATGAGCATGGAAAATGCACCACAAATTGCTGGGAAGAAAGGGAAGTAGAAAGCAGAGATGGGCAAGCAAAGCTCAAAACCAATGCGTTTTTTGCGTCCTTTGATCAACGGAGTGAAAAGGCCGCCGGAGAGAGTGCCTGCACAGCCCTTGTTGCGGTCATTGCACACTGGCTGCATTCGCACCAACACTCAATGCCTACAAGATCCAAATTCGACAGCCTCATCACACAAGGTTCCTCAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NT-C2

Protein Analysis

405

Amino Acids

44.96

Weight (kDa)

8.88

Isoelectric Point (pI)

53.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NT-C2 PF10358 31 - 163 2e-09 N-terminal C2 in EEIG1 and EHBP1 proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 412
AccB7I CCANNNNNTGG 1 cut(s) 170
AciI CCGC 5 cut(s) 27, 341, 362, 1092, 1124
AclI AACGTT 1 cut(s) 952
AclWI GGATC 2 cut(s) 760, 1167
AcoI YGGCCR 1 cut(s) 17
AcsI RAATTY 3 cut(s) 251, 894, 1178
AcuI CTGAAG 2 cut(s) 83, 577
AfaI GTAC 1 cut(s) 943
AfiI CCNNNNNNNGG 4 cut(s) 26, 170, 698, 1123
AgsI TTSAA 6 cut(s) 44, 239, 385, 448, 738, 826
AhlI ACTAGT 1 cut(s) 872
AjnI CCWGG 3 cut(s) 139, 148, 302
AloI GAACNNNNNNTCC 2 cut(s) 144, 176
AluBI AGCT 8 cut(s) 61, 67, 433, 476, 498, 588, 600, 1042
AluI AGCT 8 cut(s) 61, 67, 433, 476, 498, 588, 600, 1042
Alw26I GTCTC 2 cut(s) 420, 764
AlwI GGATC 2 cut(s) 760, 1167
Ama87I CYCGRG 2 cut(s) 784, 805
AoxI GGCC 3 cut(s) 17, 146, 1089
ApeKI GCWGC 4 cut(s) 67, 190, 530, 1141
ApoI RAATTY 3 cut(s) 251, 894, 1178
ArsI GACNNNNNNTTYG 2 cut(s) 731, 763
AspA2I CCTAGG 1 cut(s) 839
AspS9I GGNCC 3 cut(s) 146, 637, 757
AsuC2I CCSGG 2 cut(s) 656, 693
AsuHPI GGTGA 4 cut(s) 64, 130, 520, 681
AsuII TTCGAA 1 cut(s) 577
AvaI CYCGRG 2 cut(s) 784, 805
AvaII GGWCC 2 cut(s) 637, 757
AvrII CCTAGG 1 cut(s) 839
BanII GRGCYC 1 cut(s) 858
BbsI GAAGAC 2 cut(s) 838, 875
BbvCI CCTCAGC 1 cut(s) 504
BbvI GCAGC 4 cut(s) 54, 202, 542, 1128
BccI CCATC 1 cut(s) 1022
BceAI ACGGC 1 cut(s) 4
BciT130I CCWGG 3 cut(s) 141, 150, 304
BclI TGATCA 1 cut(s) 1072
BcnI CCSGG 2 cut(s) 656, 693
BcoDI GTCTC 2 cut(s) 420, 764
BcuI ACTAGT 1 cut(s) 872
BfaI CTAG 3 cut(s) 389, 840, 873
BglI GCCNNNNNGGC 1 cut(s) 145
BisI GCNGC 5 cut(s) 68, 191, 531, 1092, 1142
BlnI CCTAGG 1 cut(s) 839
BlsI GCNGC 5 cut(s) 69, 192, 532, 1093, 1143
Bme1390I CCNGG 5 cut(s) 141, 150, 304, 656, 693
Bme18I GGWCC 2 cut(s) 637, 757
BmeT110I CYCGRG 2 cut(s) 784, 805
BmgT120I GGNCC 3 cut(s) 146, 637, 757
BmiI GGNNCC 3 cut(s) 162, 855, 1204
BmrFI CCNGG 5 cut(s) 141, 150, 304, 656, 693
BmsI GCATC 1 cut(s) 410
BpiI GAAGAC 2 cut(s) 838, 875
BpmI CTGGAG 2 cut(s) 162, 171
Bpu10I CCTNAGC 1 cut(s) 504
Bpu14I TTCGAA 1 cut(s) 577
BpuMI CCSGG 2 cut(s) 656, 693
BsaJI CCNNGG 6 cut(s) 14, 302, 303, 640, 692, 839
BsaXI ACNNNNNCTCC 2 cut(s) 144, 174
Bsc4I CCNNNNNNNGG 4 cut(s) 26, 170, 698, 1123
Bse1I ACTGG 1 cut(s) 1142
Bse3DI GCAATG 2 cut(s) 622, 1128
BseBI CCWGG 3 cut(s) 141, 150, 304
BseDI CCNNGG 6 cut(s) 14, 302, 303, 640, 692, 839
BseGI GGATG 1 cut(s) 250
BseLI CCNNNNNNNGG 4 cut(s) 26, 170, 698, 1123
BseMI GCAATG 2 cut(s) 622, 1128
BseMII CTCAG 6 cut(s) 431, 495, 693, 752, 788, 928
BseNI ACTGG 1 cut(s) 1142
BseXI GCAGC 4 cut(s) 54, 202, 542, 1128
BseYI CCCAGC 2 cut(s) 467, 999
BsgI GTGCAG 1 cut(s) 1093
BshFI GGCC 3 cut(s) 19, 148, 1091
BsiHKCI CYCGRG 2 cut(s) 784, 805
BsiSI CCGG 3 cut(s) 655, 692, 1095
BslFI GGGAC 5 cut(s) 285, 385, 472, 671, 893
BslI CCNNNNNNNGG 4 cut(s) 26, 170, 698, 1123
BsmAI GTCTC 2 cut(s) 420, 764
BsmFI GGGAC 5 cut(s) 285, 385, 472, 671, 893
BsmI GAATGC 1 cut(s) 1144
BsnI GGCC 3 cut(s) 19, 148, 1091
BsoBI CYCGRG 2 cut(s) 784, 805
Bsp119I TTCGAA 1 cut(s) 577
Bsp1286I GDGCHC 1 cut(s) 858
Bsp143I GATC 5 cut(s) 179, 752, 967, 1072, 1172
BspACI CCGC 5 cut(s) 27, 341, 362, 1092, 1124
BspANI GGCC 3 cut(s) 19, 148, 1091
BspCNI CTCAG 6 cut(s) 430, 496, 692, 753, 787, 927
BspHI TCATGA 2 cut(s) 766, 970
BspLI GGNNCC 3 cut(s) 162, 855, 1204
BspPI GGATC 2 cut(s) 760, 1167
BspT104I TTCGAA 1 cut(s) 577
BsrDI GCAATG 2 cut(s) 622, 1128
BsrI ACTGG 1 cut(s) 1142
BssECI CCNNGG 6 cut(s) 14, 302, 303, 640, 692, 839
BssMI GATC 5 cut(s) 179, 752, 967, 1072, 1172
BssT1I CCWWGG 1 cut(s) 839
Bst2UI CCWGG 3 cut(s) 141, 150, 304
Bst4CI ACNGT 2 cut(s) 376, 713
Bst6I CTCTTC 4 cut(s) 574, 620, 788, 806
BstBI TTCGAA 1 cut(s) 577
BstC8I GCNNGC 3 cut(s) 59, 1035, 1108
BstDEI CTNAG 9 cut(s) 135, 417, 494, 504, 679, 761, 774, 914, 1207
BstDSI CCRYGG 1 cut(s) 14
BstEII GGTNACC 1 cut(s) 687
BstF5I GGATG 1 cut(s) 250
BstKTI GATC 5 cut(s) 182, 755, 970, 1075, 1175
BstMAI GTCTC 2 cut(s) 420, 764
BstMBI GATC 5 cut(s) 179, 752, 967, 1072, 1172
BstMWI GCNNNNNNNGC 9 cut(s) 67, 145, 398, 1039, 1061, 1112, 1121, 1130, 1147
BstNI CCWGG 3 cut(s) 141, 150, 304
BstPI GGTNACC 1 cut(s) 687
BstSCI CCNGG 5 cut(s) 139, 148, 302, 654, 691
BstV1I GCAGC 4 cut(s) 54, 202, 542, 1128
BstV2I GAAGAC 2 cut(s) 838, 875
BstX2I RGATCY 1 cut(s) 1172
BstYI RGATCY 1 cut(s) 1172
BsuRI GGCC 3 cut(s) 19, 148, 1091
BtgI CCRYGG 1 cut(s) 14
BtsCI GGATG 1 cut(s) 250
BtsI GCAGTG 1 cut(s) 894
BtsIMutI CAGTG 4 cut(s) 718, 894, 922, 1135
Cac8I GCNNGC 3 cut(s) 59, 1035, 1108
CciI TCATGA 2 cut(s) 766, 970
Cfr13I GGNCC 3 cut(s) 146, 637, 757
CseI GACGC 1 cut(s) 1053
Csp6I GTAC 1 cut(s) 942
CviAII CATG 5 cut(s) 521, 646, 767, 971, 977
CviQI GTAC 1 cut(s) 942
DdeI CTNAG 9 cut(s) 135, 417, 494, 504, 679, 761, 774, 914, 1207
DpnI GATC 5 cut(s) 181, 754, 969, 1074, 1174
DpnII GATC 5 cut(s) 179, 752, 967, 1072, 1172
DrdI GACNNNNNNGTC 1 cut(s) 412
DseDI GACNNNNNNGTC 1 cut(s) 412
EaeI YGGCCR 1 cut(s) 17
Eam1104I CTCTTC 4 cut(s) 574, 620, 788, 806
EarI CTCTTC 4 cut(s) 574, 620, 788, 806
EciI GGCGGA 1 cut(s) 42
Eco130I CCWWGG 1 cut(s) 839
Eco24I GRGCYC 1 cut(s) 858
Eco32I GATATC 1 cut(s) 933
Eco47I GGWCC 2 cut(s) 637, 757
Eco57I CTGAAG 2 cut(s) 83, 577
Eco88I CYCGRG 2 cut(s) 784, 805
Eco91I GGTNACC 1 cut(s) 687
EcoO109I RGGNCCY 1 cut(s) 146
EcoO65I GGTNACC 1 cut(s) 687
EcoRII CCWGG 3 cut(s) 139, 148, 302
EcoRV GATATC 1 cut(s) 933
EcoT14I CCWWGG 1 cut(s) 839
EcoT38I GRGCYC 1 cut(s) 858
ErhI CCWWGG 1 cut(s) 839
FaeI CATG 5 cut(s) 524, 649, 770, 974, 980
FaiI YATR 8 cut(s) 171, 359, 522, 647, 768, 926, 972, 978
FalI AAGNNNNNCTT 2 cut(s) 465, 497
FaqI GGGAC 5 cut(s) 285, 385, 472, 671, 893
FatI CATG 5 cut(s) 520, 645, 766, 970, 976
FbaI TGATCA 1 cut(s) 1072
Fnu4HI GCNGC 5 cut(s) 68, 191, 531, 1092, 1142
FokI GGATG 1 cut(s) 257
FriOI GRGCYC 1 cut(s) 858
Fsp4HI GCNGC 5 cut(s) 68, 191, 531, 1092, 1142
FspBI CTAG 3 cut(s) 389, 840, 873
GluI GCNGC 5 cut(s) 68, 191, 531, 1092, 1142
GsaI CCCAGC 2 cut(s) 471, 1003
GsuI CTGGAG 2 cut(s) 162, 171
HaeIII GGCC 3 cut(s) 19, 148, 1091
HapII CCGG 3 cut(s) 655, 692, 1095
HgaI GACGC 1 cut(s) 1053
Hin1II CATG 5 cut(s) 524, 649, 770, 974, 980
HincII GTYRAC 2 cut(s) 709, 904
HindII GTYRAC 2 cut(s) 709, 904
HindIII AAGCTT 1 cut(s) 496
HpaI GTTAAC 1 cut(s) 709
HpaII CCGG 3 cut(s) 655, 692, 1095
HphI GGTGA 4 cut(s) 64, 130, 520, 681
Hpy166II GTNNAC 2 cut(s) 709, 904
Hpy188I TCNGA 8 cut(s) 258, 420, 426, 559, 676, 682, 967, 1210
Hpy188III TCNNGA 7 cut(s) 542, 595, 750, 760, 767, 805, 971
Hpy8I GTNNAC 2 cut(s) 709, 904
Hpy99I CGWCG 1 cut(s) 187
HpyAV CCTTC 4 cut(s) 43, 82, 88, 677
HpyCH4III ACNGT 2 cut(s) 376, 713
HpyCH4IV ACGT 1 cut(s) 952
HpyCH4V TGCA 9 cut(s) 70, 520, 725, 887, 892, 987, 1110, 1133, 1144
HpyF10VI GCNNNNNNNGC 9 cut(s) 67, 145, 398, 1039, 1061, 1112, 1121, 1130, 1147
HpyF3I CTNAG 9 cut(s) 135, 417, 494, 504, 679, 761, 774, 914, 1207
HpySE526I ACGT 1 cut(s) 952
Hsp92II CATG 5 cut(s) 524, 649, 770, 974, 980
Ksp22I TGATCA 1 cut(s) 1072
KspAI GTTAAC 1 cut(s) 709
Kzo9I GATC 5 cut(s) 179, 752, 967, 1072, 1172
LmnI GCTCC 3 cut(s) 343, 597, 853
Lsp1109I GCAGC 4 cut(s) 54, 202, 542, 1128
LweI GCATC 1 cut(s) 410
MaeI CTAG 3 cut(s) 389, 840, 873
MaeII ACGT 1 cut(s) 952
MaeIII GTNAC 4 cut(s) 324, 439, 650, 687
MalI GATC 5 cut(s) 181, 754, 969, 1074, 1174
MboI GATC 5 cut(s) 179, 752, 967, 1072, 1172
MflI RGATCY 1 cut(s) 1172
MhlI GDGCHC 1 cut(s) 858
MluCI AATT 8 cut(s) 79, 251, 268, 395, 894, 937, 994, 1178
MmeI TCCRAC 1 cut(s) 699
MseI TTAA 2 cut(s) 78, 708
MslI CAYNNNNRTG 1 cut(s) 975
MspA1I CMGCKG 1 cut(s) 61
MspI CCGG 3 cut(s) 655, 692, 1095
MspR9I CCNGG 5 cut(s) 141, 150, 304, 656, 693
Mva1269I GAATGC 1 cut(s) 1144
MvaI CCWGG 3 cut(s) 141, 150, 304
MwoI GCNNNNNNNGC 9 cut(s) 67, 145, 398, 1039, 1061, 1112, 1121, 1130, 1147
NciI CCSGG 2 cut(s) 656, 693
NdeII GATC 5 cut(s) 179, 752, 967, 1072, 1172
NlaIII CATG 5 cut(s) 524, 649, 770, 974, 980
NlaIV GGNNCC 3 cut(s) 162, 855, 1204
NmeAIII GCCGAG 1 cut(s) 506
NmuCI GTSAC 2 cut(s) 650, 687
NspV TTCGAA 1 cut(s) 577
PaeR7I CTCGAG 1 cut(s) 805
PagI TCATGA 2 cut(s) 766, 970
PasI CCCWGGG 1 cut(s) 303
PcsI WCGNNNNNNNCGW 2 cut(s) 17, 191
PctI GAATGC 1 cut(s) 1144
PfeI GAWTC 2 cut(s) 421, 920
PflMI CCANNNNNTGG 1 cut(s) 170
PkrI GCNGC 5 cut(s) 69, 192, 532, 1093, 1143
Psp1406I AACGTT 1 cut(s) 952
Psp6I CCWGG 3 cut(s) 139, 148, 302
PspEI GGTNACC 1 cut(s) 687
PspFI CCCAGC 2 cut(s) 467, 999
PspGI CCWGG 3 cut(s) 139, 148, 302
PspN4I GGNNCC 3 cut(s) 162, 855, 1204
PspPI GGNCC 3 cut(s) 146, 637, 757
PsuI RGATCY 1 cut(s) 1172
PvuII CAGCTG 1 cut(s) 61
RsaI GTAC 1 cut(s) 943
RsaNI GTAC 1 cut(s) 942
RseI CAYNNNNRTG 1 cut(s) 975
SaqAI TTAA 2 cut(s) 78, 708
SatI GCNGC 5 cut(s) 68, 191, 531, 1092, 1142
Sau3AI GATC 5 cut(s) 179, 752, 967, 1072, 1172
Sau96I GGNCC 3 cut(s) 146, 637, 757
ScrFI CCNGG 5 cut(s) 141, 150, 304, 656, 693
SduI GDGCHC 1 cut(s) 858
SfaNI GCATC 1 cut(s) 410
Sfr274I CTCGAG 1 cut(s) 805
SfuI TTCGAA 1 cut(s) 577
SinI GGWCC 2 cut(s) 637, 757
SlaI CTCGAG 1 cut(s) 805
SmiMI CAYNNNNRTG 1 cut(s) 975
SmlI CTYRAG 1 cut(s) 805
SmoI CTYRAG 1 cut(s) 805
SpeI ACTAGT 1 cut(s) 872
Sse9I AATT 8 cut(s) 79, 251, 268, 395, 894, 937, 994, 1178
SsiI CCGC 5 cut(s) 27, 341, 362, 1092, 1124
SspMI CTAG 3 cut(s) 389, 840, 873
StyD4I CCNGG 5 cut(s) 139, 148, 302, 654, 691
StyI CCWWGG 1 cut(s) 839
TaaI ACNGT 2 cut(s) 376, 713
TaiI ACGT 1 cut(s) 955
TaqI TCGA 7 cut(s) 185, 278, 404, 548, 577, 806, 1182
TaqII GACCGA 3 cut(s) 527, 654, 745
TasI AATT 8 cut(s) 79, 251, 268, 395, 894, 937, 994, 1178
TatI WGTACW 1 cut(s) 941
TauI GCSGC 1 cut(s) 1094
TfiI GAWTC 2 cut(s) 421, 920
Tru1I TTAA 2 cut(s) 78, 708
Tru9I TTAA 2 cut(s) 78, 708
TscAI CASTG 4 cut(s) 718, 894, 922, 1142
TseFI GTSAC 2 cut(s) 650, 687
TseI GCWGC 4 cut(s) 67, 190, 530, 1141
Tsp45I GTSAC 2 cut(s) 650, 687
TspDTI ATGAA 2 cut(s) 17, 264
TspGWI ACGGA 3 cut(s) 345, 889, 1093
TspRI CASTG 4 cut(s) 718, 894, 922, 1142
Van91I CCANNNNNTGG 1 cut(s) 170
VpaK11BI GGWCC 2 cut(s) 637, 757
XapI RAATTY 3 cut(s) 251, 894, 1178
XhoI CTCGAG 1 cut(s) 805
XmaJI CCTAGG 1 cut(s) 839
XspI CTAG 3 cut(s) 389, 840, 873
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.