pycom15g12100

Protein trichome birefringence-like 14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008475
Physical Location & Seq
Reverse (-)
8239054 .. 8243862
4809 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1389 bp
ATGAAAGGGGGATATAACTACAAATTGATTGGGAAACCAATCTCTTTCACTCTGGCTGCCCTTGTATTGACAACAATATTACTTTGGGCGTGGGAGAAAAATCCTTTTGCTGATACTCTACTATTGCCGCACCAGCGGTTTCCAATGCCTTCCATAGATTTTTTTGGGGACTTCTCAAATAATTCCTCCAAACCTATGAAGACAAATGGAAATATTAAAGGGAAGGATTTACATTTGAATACAGCAGAAGATACAAAAGTAGTAGAAAAGTTTGGCACTGCCCCAATAGATTCTATGTATGCATTCTCTCCTGGAATGCAAGAAAGTAATGGGAATGCGACATCTTTCCAAACTAAAGTGTGTGATTATGCCAAGGGTAGATGGGTTGCAGACAGCAGGCACCCTTTATATTCTGGGTTTAAATGTAAGCAGTGGTTATCAGAAATGTGGGCGTGTAGATTGACACAACGAACAGATTTTTCTTTTGAGGGTTACCGATGGAAGCCAGCAAATTGTGGAATACCAGATTTTGAACGCTCTGCATTCTTGAGAAGGATGCAGGACAAAACGATTGCTTTCATAGGAGATTCATTGGGTAGACAGCAGTTCCAGTCTTTGATGTGTATGACAACTGGCGGAGAAGAAAGCCCCGAAGTTGAGAATGTCGGAAAGGAATATGGCCTTGTCAAACGTCGGGGAGCCATTCGTCCTGATGGCTGGGCTTATCGATTCTCTAACACCAATACCACTATCTTATATTATTGGTCAGCAAGCCTGTGTGATCTAGTGCCCCTTAACATCTCAGACCGAGCCAGTGATATTGCCATGCATTTGGATCGTCCCCCAGCTTTCTTGAGACAATACCTTCACCGGTTTGATGTGCTGGTTCTTAATACAGGGCATCATTGGAATAGGGGAAAGATTAATGGTAACCGGTGGGTAATGTATGTAAATGGAAAGCCCAATCAAGACAAGAAGCGTGCAGAAATTGGGAATGCTAAGAATTTTACTGTGTACAGTATTGCCAGGTGGGTTGATTCACAACTACCATCACATCCCCGCCTAAAAGCTTTCTTTCGGACTATTTCACCAAGGCATTTCTTCAATGGTGACTGGAATACTGGGGGTACCTGCGACAATACTACTCCGTTGGTAGGAGGAAGTGAAGTAGTCCAGGAAGGATCCAGTGATTCAGTGATCGAGGGTGCTTTGAAAGGTACAAAAGTAAAGTTACTGGATATAACTGCCATTTCTCAATTGAGAGACGAAGGTCACATATCCCACTATACCATTAGAGGAAAAGGAAATGCAGGCACAAATGATTGCTTACACTGGTGCTTACCTGGAATTCCAGATGCATGGAATGAACTCCTTGTTGCGCAGATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PC-Esterase PMR5N

Protein Analysis

463

Amino Acids

52.15

Weight (kDa)

9.05

Isoelectric Point (pI)

37.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMR5N PF14416 120 - 172 3.1e-15 PMR5 N terminal Domain
PC-Esterase PF13839 174 - 460 4.9e-74 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1380
Acc36I ACCTGC 1 cut(s) 1139
Acc65I GGTACC 1 cut(s) 1127
AccB1I GGYRCC 2 cut(s) 399, 1127
AccI GTMKAC 1 cut(s) 598
AciI CCGC 4 cut(s) 128, 136, 636, 1060
AclWI GGATC 3 cut(s) 843, 1176, 1189
AcsI RAATTY 2 cut(s) 1003, 1347
AfaI GTAC 3 cut(s) 1016, 1129, 1219
AfiI CCNNNNNNNGG 1 cut(s) 1154
AgeI ACCGGT 2 cut(s) 870, 933
AgsI TTSAA 4 cut(s) 238, 533, 1105, 1213
AjnI CCWGG 4 cut(s) 310, 1025, 1173, 1342
AluBI AGCT 2 cut(s) 848, 1070
AluI AGCT 2 cut(s) 848, 1070
Alw26I GTCTC 2 cut(s) 850, 1257
AlwI GGATC 3 cut(s) 843, 1176, 1189
AoxI GGCC 1 cut(s) 679
ApeKI GCWGC 1 cut(s) 56
ApoI RAATTY 2 cut(s) 1003, 1347
AseI ATTAAT 1 cut(s) 924
AsiGI ACCGGT 2 cut(s) 870, 933
Asp718I GGTACC 1 cut(s) 1127
AspLEI GCGC 1 cut(s) 1381
AsuHPI GGTGA 3 cut(s) 860, 1080, 1121
BaeGI GKGCMC 1 cut(s) 792
BaeI ACNNNNGTAYC 4 cut(s) 243, 276, 1209, 1242
BamHI GGATCC 1 cut(s) 1181
BanI GGYRCC 2 cut(s) 399, 1127
BbsI GAAGAC 1 cut(s) 206
BbvI GCAGC 1 cut(s) 43
BccI CCATC 4 cut(s) 375, 492, 707, 1057
BcgI CGANNNNNNTGC 2 cut(s) 818, 852
BciT130I CCWGG 4 cut(s) 312, 1027, 1175, 1344
BcoDI GTCTC 2 cut(s) 850, 1257
BfaI CTAG 1 cut(s) 785
BfuAI ACCTGC 1 cut(s) 1139
BisI GCNGC 2 cut(s) 57, 128
BlsI GCNGC 2 cut(s) 58, 129
Bme1390I CCNGG 4 cut(s) 312, 1027, 1175, 1344
BmiI GGNNCC 4 cut(s) 401, 700, 1129, 1183
BmrFI CCNGG 4 cut(s) 312, 1027, 1175, 1344
BmrI ACTGGG 1 cut(s) 1131
BmsI GCATC 3 cut(s) 546, 910, 1345
BmuI ACTGGG 1 cut(s) 1131
BoxI GACNNNNGTC 1 cut(s) 1269
BpiI GAAGAC 1 cut(s) 206
BpuEI CTTGAG 2 cut(s) 568, 874
Bsa29I ATCGAT 1 cut(s) 727
BsaJI CCNNGG 2 cut(s) 372, 1091
BsaWI WCCGGW 2 cut(s) 870, 933
Bsc4I CCNNNNNNNGG 1 cut(s) 1154
Bse118I RCCGGY 2 cut(s) 870, 933
Bse1I ACTGG 8 cut(s) 610, 637, 813, 1118, 1126, 1185, 1239, 1337
BseBI CCWGG 4 cut(s) 312, 1027, 1175, 1344
BseCI ATCGAT 1 cut(s) 727
BseDI CCNNGG 2 cut(s) 372, 1091
BseGI GGATG 2 cut(s) 561, 1054
BseLI CCNNNNNNNGG 1 cut(s) 1154
BseMII CTCAG 1 cut(s) 816
BseNI ACTGG 8 cut(s) 610, 637, 813, 1118, 1126, 1185, 1239, 1337
BseSI GKGCMC 1 cut(s) 792
BseXI GCAGC 1 cut(s) 43
BseYI CCCAGC 2 cut(s) 717, 844
BsgI GTGCAG 1 cut(s) 1002
BshFI GGCC 1 cut(s) 681
BshNI GGYRCC 2 cut(s) 399, 1127
BshTI ACCGGT 2 cut(s) 870, 933
BshVI ATCGAT 1 cut(s) 727
BsiSI CCGG 2 cut(s) 871, 934
BslFI GGGAC 2 cut(s) 182, 825
BslI CCNNNNNNNGG 1 cut(s) 1154
BsmAI GTCTC 2 cut(s) 850, 1257
BsmBI CGTCTC 1 cut(s) 1257
BsmFI GGGAC 2 cut(s) 182, 825
BsmI GAATGC 5 cut(s) 302, 321, 340, 542, 1000
BsnI GGCC 1 cut(s) 681
Bsp1286I GDGCHC 1 cut(s) 792
Bsp1407I TGTACA 1 cut(s) 1014
Bsp143I GATC 4 cut(s) 781, 835, 1181, 1197
BspACI CCGC 4 cut(s) 128, 136, 636, 1060
BspANI GGCC 1 cut(s) 681
BspCNI CTCAG 1 cut(s) 815
BspDI ATCGAT 1 cut(s) 727
BspLI GGNNCC 4 cut(s) 401, 700, 1129, 1183
BspMI ACCTGC 1 cut(s) 1139
BspPI GGATC 3 cut(s) 843, 1176, 1189
BspT107I GGYRCC 2 cut(s) 399, 1127
BsrFI RCCGGY 2 cut(s) 870, 933
BsrGI TGTACA 1 cut(s) 1014
BsrI ACTGG 8 cut(s) 610, 637, 813, 1118, 1126, 1185, 1239, 1337
BssAI RCCGGY 2 cut(s) 870, 933
BssECI CCNNGG 2 cut(s) 372, 1091
BssMI GATC 4 cut(s) 781, 835, 1181, 1197
BssT1I CCWWGG 2 cut(s) 372, 1091
Bst2UI CCWGG 4 cut(s) 312, 1027, 1175, 1344
Bst4CI ACNGT 2 cut(s) 1012, 1019
BstAUI TGTACA 1 cut(s) 1014
BstC8I GCNNGC 5 cut(s) 398, 507, 772, 981, 1312
BstDEI CTNAG 2 cut(s) 802, 999
BstEII GGTNACC 2 cut(s) 491, 929
BstF5I GGATG 2 cut(s) 561, 1054
BstHHI GCGC 1 cut(s) 1381
BstKTI GATC 4 cut(s) 784, 838, 1184, 1200
BstMAI GTCTC 2 cut(s) 850, 1257
BstMBI GATC 4 cut(s) 781, 835, 1181, 1197
BstMWI GCNNNNNNNGC 1 cut(s) 133
BstNI CCWGG 4 cut(s) 312, 1027, 1175, 1344
BstPAI GACNNNNGTC 1 cut(s) 1269
BstPI GGTNACC 2 cut(s) 491, 929
BstSCI CCNGG 4 cut(s) 310, 1025, 1173, 1342
BstSLI GKGCMC 1 cut(s) 792
BstV1I GCAGC 1 cut(s) 43
BstV2I GAAGAC 1 cut(s) 206
BstX2I RGATCY 1 cut(s) 1181
BstXI CCANNNNNNTGG 2 cut(s) 832, 1359
BstYI RGATCY 1 cut(s) 1181
Bsu15I ATCGAT 1 cut(s) 727
BsuRI GGCC 1 cut(s) 681
BsuTUI ATCGAT 1 cut(s) 727
BtsCI GGATG 2 cut(s) 561, 1054
BtsI GCAGTG 2 cut(s) 276, 437
BtsIMutI CAGTG 6 cut(s) 276, 437, 820, 1192, 1200, 1330
BveI ACCTGC 1 cut(s) 1139
Cac8I GCNNGC 5 cut(s) 398, 507, 772, 981, 1312
CfoI GCGC 1 cut(s) 1381
Cfr10I RCCGGY 2 cut(s) 870, 933
ClaI ATCGAT 1 cut(s) 727
Csp6I GTAC 3 cut(s) 1015, 1128, 1218
CspAI ACCGGT 2 cut(s) 870, 933
CviAII CATG 2 cut(s) 826, 1359
CviQI GTAC 3 cut(s) 1015, 1128, 1218
DdeI CTNAG 2 cut(s) 802, 999
DpnI GATC 4 cut(s) 783, 837, 1183, 1199
DpnII GATC 4 cut(s) 781, 835, 1181, 1197
DraI TTTAAA 1 cut(s) 421
EciI GGCGGA 1 cut(s) 651
Eco130I CCWWGG 2 cut(s) 372, 1091
Eco91I GGTNACC 2 cut(s) 491, 929
EcoO65I GGTNACC 2 cut(s) 491, 929
EcoRI GAATTC 1 cut(s) 1347
EcoRII CCWGG 4 cut(s) 310, 1025, 1173, 1342
EcoT14I CCWWGG 2 cut(s) 372, 1091
EcoT22I ATGCAT 3 cut(s) 304, 831, 1360
ErhI CCWWGG 2 cut(s) 372, 1091
Esp3I CGTCTC 1 cut(s) 1257
FaeI CATG 2 cut(s) 829, 1362
FaqI GGGAC 2 cut(s) 182, 825
FatI CATG 2 cut(s) 825, 1358
FauI CCCGC 1 cut(s) 1067
FblI GTMKAC 1 cut(s) 598
Fnu4HI GCNGC 2 cut(s) 57, 128
FokI GGATG 2 cut(s) 568, 1041
Fsp4HI GCNGC 2 cut(s) 57, 128
FspBI CTAG 1 cut(s) 785
FspI TGCGCA 1 cut(s) 1380
GlaI GCGC 1 cut(s) 1380
GluI GCNGC 2 cut(s) 57, 128
GsaI CCCAGC 2 cut(s) 721, 848
HaeIII GGCC 1 cut(s) 681
HapII CCGG 2 cut(s) 871, 934
HhaI GCGC 1 cut(s) 1381
Hin1II CATG 2 cut(s) 829, 1362
Hin6I GCGC 1 cut(s) 1379
HinP1I GCGC 1 cut(s) 1379
HindIII AAGCTT 1 cut(s) 1068
HinfI GANTC 5 cut(s) 290, 587, 729, 1037, 1190
HpaII CCGG 2 cut(s) 871, 934
HphI GGTGA 3 cut(s) 860, 1080, 1121
Hpy166II GTNNAC 2 cut(s) 599, 1015
Hpy188I TCNGA 4 cut(s) 442, 668, 805, 1080
Hpy188III TCNNGA 5 cut(s) 547, 710, 853, 968, 1352
Hpy8I GTNNAC 2 cut(s) 599, 1015
Hpy99I CGWCG 1 cut(s) 696
HpyAV CCTTC 6 cut(s) 159, 217, 546, 875, 1172, 1262
HpyCH4III ACNGT 2 cut(s) 1012, 1019
HpyCH4IV ACGT 1 cut(s) 691
HpyCH4V TGCA 9 cut(s) 302, 319, 389, 542, 559, 829, 983, 1310, 1358
HpyF10VI GCNNNNNNNGC 1 cut(s) 133
HpyF3I CTNAG 2 cut(s) 802, 999
HpySE526I ACGT 1 cut(s) 691
Hsp92II CATG 2 cut(s) 829, 1362
HspAI GCGC 1 cut(s) 1379
KpnI GGTACC 1 cut(s) 1131
Kzo9I GATC 4 cut(s) 781, 835, 1181, 1197
LmnI GCTCC 1 cut(s) 698
Lsp1109I GCAGC 1 cut(s) 43
LweI GCATC 3 cut(s) 546, 910, 1345
MaeI CTAG 1 cut(s) 785
MaeII ACGT 1 cut(s) 691
MaeIII GTNAC 5 cut(s) 491, 929, 1109, 1230, 1271
MalI GATC 4 cut(s) 783, 837, 1183, 1199
MboI GATC 4 cut(s) 781, 835, 1181, 1197
MboII GAAGA 4 cut(s) 211, 260, 653, 1093
MfeI CAATTG 1 cut(s) 1256
MflI RGATCY 1 cut(s) 1181
MhlI GDGCHC 1 cut(s) 792
MluCI AATT 7 cut(s) 23, 181, 511, 987, 1003, 1256, 1347
MmeI TCCRAC 1 cut(s) 646
MnlI CCTC 5 cut(s) 196, 481, 1151, 1195, 1289
Mph1103I ATGCAT 3 cut(s) 304, 831, 1360
MseI TTAA 5 cut(s) 216, 420, 795, 891, 924
MspA1I CMGCKG 1 cut(s) 136
MspI CCGG 2 cut(s) 871, 934
MspR9I CCNGG 4 cut(s) 312, 1027, 1175, 1344
MunI CAATTG 1 cut(s) 1256
Mva1269I GAATGC 5 cut(s) 302, 321, 340, 542, 1000
MvaI CCWGG 4 cut(s) 312, 1027, 1175, 1344
MwoI GCNNNNNNNGC 1 cut(s) 133
NdeII GATC 4 cut(s) 781, 835, 1181, 1197
NlaIII CATG 2 cut(s) 829, 1362
NlaIV GGNNCC 4 cut(s) 401, 700, 1129, 1183
NmuCI GTSAC 2 cut(s) 1109, 1271
NsbI TGCGCA 1 cut(s) 1380
NsiI ATGCAT 3 cut(s) 304, 831, 1360
PctI GAATGC 5 cut(s) 302, 321, 340, 542, 1000
PfeI GAWTC 5 cut(s) 290, 587, 729, 1037, 1190
PfoI TCCNGGA 2 cut(s) 310, 1173
PinAI ACCGGT 2 cut(s) 870, 933
PkrI GCNGC 2 cut(s) 58, 129
PshAI GACNNNNGTC 1 cut(s) 1269
PshBI ATTAAT 1 cut(s) 924
Psp6I CCWGG 4 cut(s) 310, 1025, 1173, 1342
PspEI GGTNACC 2 cut(s) 491, 929
PspFI CCCAGC 2 cut(s) 717, 844
PspGI CCWGG 4 cut(s) 310, 1025, 1173, 1342
PspN4I GGNNCC 4 cut(s) 401, 700, 1129, 1183
PsuI RGATCY 1 cut(s) 1181
RsaI GTAC 3 cut(s) 1016, 1129, 1219
RsaNI GTAC 3 cut(s) 1015, 1128, 1218
SaqAI TTAA 5 cut(s) 216, 420, 795, 891, 924
SatI GCNGC 2 cut(s) 57, 128
Sau3AI GATC 4 cut(s) 781, 835, 1181, 1197
ScrFI CCNGG 4 cut(s) 312, 1027, 1175, 1344
SduI GDGCHC 1 cut(s) 792
SfaNI GCATC 3 cut(s) 546, 910, 1345
SmlI CTYRAG 2 cut(s) 547, 853
SmoI CTYRAG 2 cut(s) 547, 853
Sse9I AATT 7 cut(s) 23, 181, 511, 987, 1003, 1256, 1347
SsiI CCGC 4 cut(s) 128, 136, 636, 1060
SspI AATATT 2 cut(s) 78, 214
SspMI CTAG 1 cut(s) 785
StyD4I CCNGG 4 cut(s) 310, 1025, 1173, 1342
StyI CCWWGG 2 cut(s) 372, 1091
TaaI ACNGT 2 cut(s) 1012, 1019
TaiI ACGT 1 cut(s) 694
TaqI TCGA 2 cut(s) 727, 1200
TaqII GACCGA 1 cut(s) 822
TasI AATT 7 cut(s) 23, 181, 511, 987, 1003, 1256, 1347
TatI WGTACW 1 cut(s) 1014
TauI GCSGC 1 cut(s) 130
TfiI GAWTC 5 cut(s) 290, 587, 729, 1037, 1190
Tru1I TTAA 5 cut(s) 216, 420, 795, 891, 924
Tru9I TTAA 5 cut(s) 216, 420, 795, 891, 924
TscAI CASTG 6 cut(s) 283, 437, 820, 1192, 1200, 1337
TseFI GTSAC 2 cut(s) 1109, 1271
TseI GCWGC 1 cut(s) 56
Tsp45I GTSAC 2 cut(s) 1109, 1271
TspDTI ATGAA 5 cut(s) 17, 212, 568, 579, 1380
TspGWI ACGGA 1 cut(s) 1137
TspRI CASTG 6 cut(s) 283, 437, 820, 1192, 1200, 1337
VspI ATTAAT 1 cut(s) 924
XapI RAATTY 2 cut(s) 1003, 1347
XmiI GTMKAC 1 cut(s) 598
XspI CTAG 1 cut(s) 785
Zsp2I ATGCAT 3 cut(s) 304, 831, 1360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.