pycom15g12650

Domain of unknown function (DUF3475)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0010330
Physical Location & Seq
Reverse (-)
8647972 .. 8656173
8202 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1938 bp
ATGGGAGCGGTTTGTTCCAAGAAGTCGAGGAGTACGAGCGTGAAGAGCGCCGGTTGGGATATCAATCGGCAACAAGTTCAAGTTGATCAGCCCAAGTCAAGCTTGAAGACCAGTGGCGGCGTCACTCCGTCCTCGCCTCAGACTCCGCGCACGGATGTGCATTTGCAGAAAGACAAAGAGGAACAGTTATCAGACAAGGAAGACAATGAGATTCAGATTGATAGAATTAATCCTCTTGCGTATGAGTCTGACGATGAATTCTACGACGGAATTCCGCGCTATCCCGCCTCCTTGTCTCAGAAATCCAGCTCCTTTCGCTCTAAGCAGGCCGCCACTAAGGTTTCTGAGGTGAGTTCGCGTTTGGGCAGAGCTGGCAGTGCTGGCCTTGGAAAGGCAGTCCAGGTCCTGGACACACTCGGGAGCAGCATGACAAATTTGAATTCCGGCCCTGGTTTCGCTTCCGCAGCACCTGCCAAAGGCAATGAAATTGCAATCATTTCGTTTGAGATTGCCAACACTATTGTTAAGGGTTCCAATCTAATGCAATCCCTTTCCAAGCGATCTATGCGCCAGTTGAAAGAAATGGTGCTTGTTTCACAAGGTGTGCAGCATTTGGTGTCCAAAGATATGGATGAATTATTAAGGATTGTTGCAGCTGACAAAAGGGAAGAACTACAAATTTTTTCGGGTGAGGTTGTTCGTTTTGGAAATCGATGCAAGGAGTCTCAGTGGCACAACTTGGACCGATATTTTGACAAAATCAGCAGGGAACTTACCCCTCTGAAGTATCTGAAGGAGGATGCAGGTTCGATGATGCAGCAACTGATGACTTTGGTCGTGTTTACTGCTGAATTATACAATGAGTTTCATGCGTTGGACCGATTGGAACAAATCTATGCACGCAAGCGTCAGGAAGAAGGTGACAGCCTTGGAAGCTTGAGGTCAGGATTAAAAAGCCAAAGGAAAATAGTTAAATCTTTAAAGAAAAAGTCGCTTTGGTCCAAAAGTATGGAAGAGGTGATGGAGAAGCTTGTTGATATTGTCCACTTTTTACATCTAGAAATACATTCGGCCTTTGGTGGTGCTGATAGTCATGAAGCAGTGGAGGGACCTACAAGCAATCATCAAAGACTAGGACCAGCTGGACTTTCTTTACACTATGCAAATATAATCCTCCAAATTGATAGTCTTGTAGCCAAGTCAAGTTCTATGCCTCAAAATACAAAGGATACATTATACCAGAACTTGCCGCCTAATATAAAATCATCCTTGCGCTCCAAATTACAGTCTTTTCATGTTAAAGAAGAGCTTAGTATTACAGATATCAAAGCTGAGATGGAGAAAACTCTACACTGGCTTGTTCCAGTTGCTACAAACACAGCAAAAGCGCATCATGGTTTTGGCTGGGTAGGAGAGTGGGCGAGTTCTGGTTCCCAGTCAAACCGAAAGCCATTGTTGCAGACTGAAGCGATTCGAATTGAGACATTCCATCATGCAGACAAGGAAAAAACAGAAGCCTGTATACTCGATCTAATCTTATGGCTTCACCATTTGGCTTGTCAAACCAAGTCTAGTGCCGATAACGGAGGCATGAAGTCACCTGTCAAGTCCACAACCAGTACAACCCTCGTGAAGACAAATAAACAGCTGCCCACCGGTGTTCAACCAAGCATGCTAACAACTGAAGACGCGGAGATGCTGCAGGATGTGAACAAGAGAAAACGGATTCCAGGTAATAGTAAGAGTCAGGACTTTGACAGTGCCAGTTCCAGTTCAACAGAGGGCCGAAAATTGAGTAAGAGTAACAGCTACTCTCACGGGCAAGGAAGTGACAAATTGTTACATTTGAGTAGGCCCTCTTCTGGCGTTGATTTTGGTATCGATAAGGAAAAAGCACTGGATGTTATAGATAGAGTGGACGGACTCACAATCACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3475 DUF668

Protein Analysis

646

Amino Acids

71.3

Weight (kDa)

8.86

Isoelectric Point (pI)

46.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3475 PF11961 165 - 221 3.5e-21 Domain of unknown function (DUF3475)
DUF668 PF05003 377 - 462 5.4e-32 Protein of unknown function (DUF668)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010330)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08660 AT5G08660 AT5G08660 AT5G08660
fragaria_vesca FvH4_1g25750 FvH4_1g25750
malus_domestica MD15G1140000.v1.1
prunus_persica Prupe.4G285700_v2.0.a1 Prupe.4G285700_v2.0.a1 Prupe.4G285700_v2.0.a1
pyrus_communis pycom15g12650
rosa_chinensis RchiOBHm_Chr3g0484691
rosa_laevigata RLG00000023207
rosa_multiflora Rmu_sc0006192.1_g000003
rosa_roxburghii Rroxscaffold_6G00397520
rosa_rugosa Rorug03G0213500
rosa_samantha Rh3AG263600 Rh3BG298800 Rh3CG296200 Rh3DG292300
rosa_wichuraiana Rw3G023510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 478
Acc36I ACCTGC 2 cut(s) 478, 794
AccB7I CCANNNNNTGG 1 cut(s) 406
AccBSI CCGCTC 1 cut(s) 8
AccI GTMKAC 1 cut(s) 1522
AccII CGCG 4 cut(s) 148, 277, 358, 1691
AciI CCGC 9 cut(s) 8, 117, 146, 275, 285, 330, 462, 1250, 1691
AcsI RAATTY 5 cut(s) 257, 270, 433, 439, 678
AcuI CTGAAG 4 cut(s) 803, 812, 1485, 1704
AcyI GRCGYC 1 cut(s) 120
AdeI CACNNNGTG 1 cut(s) 602
AfaI GTAC 2 cut(s) 34, 1621
AfiI CCNNNNNNNGG 4 cut(s) 391, 406, 476, 1862
AgeI ACCGGT 1 cut(s) 1655
AgsI TTSAA 6 cut(s) 80, 106, 439, 577, 1664, 1776
AjnI CCWGG 4 cut(s) 399, 405, 448, 1729
AleI CACNNNNGTG 1 cut(s) 155
Alw26I GTCTC 3 cut(s) 300, 729, 1475
AlwNI CAGNNNCTG 3 cut(s) 406, 470, 823
Ama87I CYCGRG 1 cut(s) 416
AoxI GGCC 6 cut(s) 327, 382, 445, 1071, 1783, 1853
ApeKI GCWGC 7 cut(s) 423, 464, 607, 653, 817, 1648, 1699
ApoI RAATTY 5 cut(s) 257, 270, 433, 439, 678
ArsI GACNNNNNNTTYG 4 cut(s) 734, 766, 1171, 1203
AseI ATTAAT 1 cut(s) 228
AsiGI ACCGGT 1 cut(s) 1655
Asp700I GAANNNNTTC 1 cut(s) 1470
AspLEI GCGC 6 cut(s) 50, 150, 279, 570, 1275, 1390
AspS9I GGNCC 9 cut(s) 403, 446, 742, 877, 999, 1109, 1136, 1783, 1854
AsuHPI GGTGA 6 cut(s) 361, 701, 932, 1030, 1538, 1590
AsuII TTCGAA 1 cut(s) 1474
AvaI CYCGRG 1 cut(s) 416
AvaII GGWCC 6 cut(s) 403, 742, 877, 999, 1109, 1136
BarI GAAGNNNNNNTAC 4 cut(s) 1506, 1538, 1843, 1875
BauI CACGAG 1 cut(s) 1628
BbsI GAAGAC 4 cut(s) 113, 207, 1640, 1692
BbvI GCAGC 7 cut(s) 435, 476, 619, 665, 829, 1635, 1686
BccI CCATC 3 cut(s) 1015, 1330, 1497
BcgI CGANNNNNNTGC 2 cut(s) 480, 514
BciT130I CCWGG 4 cut(s) 401, 407, 450, 1731
BciVI GTATCC 1 cut(s) 1222
BclI TGATCA 1 cut(s) 85
BcoDI GTCTC 3 cut(s) 300, 729, 1475
BfaI CTAG 3 cut(s) 1058, 1133, 1572
BfmI CTRYAG 1 cut(s) 1700
BfoI RGCGCY 1 cut(s) 51
BfuAI ACCTGC 2 cut(s) 478, 794
BfuI GTATCC 1 cut(s) 1222
Bme1390I CCNGG 4 cut(s) 401, 407, 450, 1731
Bme18I GGWCC 6 cut(s) 403, 742, 877, 999, 1109, 1136
BmeT110I CYCGRG 1 cut(s) 416
BmgT120I GGNCC 9 cut(s) 403, 446, 742, 877, 999, 1109, 1136, 1783, 1854
BmiI GGNNCC 3 cut(s) 532, 1110, 1432
BmrFI CCNGG 4 cut(s) 401, 407, 450, 1731
BmrI ACTGGG 1 cut(s) 1429
BmsI GCATC 5 cut(s) 704, 790, 804, 1399, 1686
BmuI ACTGGG 1 cut(s) 1429
BoxI GACNNNNGTC 1 cut(s) 833
BpiI GAAGAC 4 cut(s) 113, 207, 1640, 1692
Bpu14I TTCGAA 1 cut(s) 1474
BpuEI CTTGAG 1 cut(s) 958
Bsa29I ATCGAT 2 cut(s) 712, 1881
BsaBI GATNNNNATC 1 cut(s) 63
BsaHI GRCGYC 1 cut(s) 120
BsaJI CCNNGG 3 cut(s) 385, 448, 928
BsaWI WCCGGW 1 cut(s) 1655
BsaXI ACNNNNNCTCC 2 cut(s) 713, 743
Bsc4I CCNNNNNNNGG 4 cut(s) 391, 406, 476, 1862
Bse118I RCCGGY 2 cut(s) 50, 1655
Bse1I ACTGG 9 cut(s) 111, 571, 1358, 1364, 1435, 1617, 1764, 1770, 1902
Bse3DI GCAATG 1 cut(s) 487
Bse8I GATNNNNATC 1 cut(s) 63
BseBI CCWGG 4 cut(s) 401, 407, 450, 1731
BseCI ATCGAT 2 cut(s) 712, 1881
BseDI CCNNGG 3 cut(s) 385, 448, 928
BseGI GGATG 6 cut(s) 160, 637, 805, 1265, 1711, 1906
BseJI GATNNNNATC 1 cut(s) 63
BseLI CCNNNNNNNGG 4 cut(s) 391, 406, 476, 1862
BseMI GCAATG 1 cut(s) 487
BseMII CTCAG 5 cut(s) 152, 311, 336, 740, 1323
BseNI ACTGG 9 cut(s) 111, 571, 1358, 1364, 1435, 1617, 1764, 1770, 1902
BseRI GAGGAG 1 cut(s) 43
BseXI GCAGC 7 cut(s) 435, 476, 619, 665, 829, 1635, 1686
BseYI CCCAGC 1 cut(s) 1404
BsgI GTGCAG 1 cut(s) 626
Bsh1236I CGCG 4 cut(s) 148, 277, 358, 1691
BshFI GGCC 6 cut(s) 329, 384, 447, 1073, 1785, 1855
BshTI ACCGGT 1 cut(s) 1655
BshVI ATCGAT 2 cut(s) 712, 1881
BsiHKCI CYCGRG 1 cut(s) 416
BsiSI CCGG 3 cut(s) 51, 444, 1656
BslFI GGGAC 1 cut(s) 1122
BslI CCNNNNNNNGG 4 cut(s) 391, 406, 476, 1862
BsmAI GTCTC 3 cut(s) 300, 729, 1475
BsmFI GGGAC 1 cut(s) 1122
BsnI GGCC 6 cut(s) 329, 384, 447, 1073, 1785, 1855
BsoBI CYCGRG 1 cut(s) 416
Bsp119I TTCGAA 1 cut(s) 1474
Bsp143I GATC 3 cut(s) 85, 560, 1528
BspACI CCGC 9 cut(s) 8, 117, 146, 275, 285, 330, 462, 1250, 1691
BspANI GGCC 6 cut(s) 329, 384, 447, 1073, 1785, 1855
BspCNI CTCAG 5 cut(s) 151, 310, 337, 739, 1324
BspDI ATCGAT 2 cut(s) 712, 1881
BspFNI CGCG 4 cut(s) 148, 277, 358, 1691
BspHI TCATGA 1 cut(s) 1093
BspLI GGNNCC 3 cut(s) 532, 1110, 1432
BspMAI CTGCAG 1 cut(s) 1704
BspMI ACCTGC 2 cut(s) 478, 794
BspQI GCTCTTC 2 cut(s) 38, 1299
BspT104I TTCGAA 1 cut(s) 1474
BsrBI CCGCTC 1 cut(s) 8
BsrDI GCAATG 1 cut(s) 487
BsrFI RCCGGY 2 cut(s) 50, 1655
BsrI ACTGG 9 cut(s) 111, 571, 1358, 1364, 1435, 1617, 1764, 1770, 1902
BssAI RCCGGY 2 cut(s) 50, 1655
BssECI CCNNGG 3 cut(s) 385, 448, 928
BssMI GATC 3 cut(s) 85, 560, 1528
BssNAI GTATAC 1 cut(s) 1523
BssNI GRCGYC 1 cut(s) 120
BssSI CACGAG 1 cut(s) 1628
BssT1I CCWWGG 2 cut(s) 385, 928
Bst1107I GTATAC 1 cut(s) 1523
Bst2BI CACGAG 1 cut(s) 1628
Bst2UI CCWGG 4 cut(s) 401, 407, 450, 1731
Bst4CI ACNGT 3 cut(s) 186, 1287, 1760
Bst6I CTCTTC 4 cut(s) 38, 1008, 1299, 1864
BstACI GRCGYC 1 cut(s) 120
BstAPI GCANNNNNTGC 1 cut(s) 470
BstBI TTCGAA 1 cut(s) 1474
BstC8I GCNNGC 6 cut(s) 327, 373, 382, 901, 905, 1673
BstDEI CTNAG 8 cut(s) 138, 297, 321, 336, 345, 726, 1310, 1332
BstENI CCTNNNNNAGG 2 cut(s) 389, 474
BstF5I GGATG 6 cut(s) 160, 637, 805, 1265, 1711, 1906
BstFNI CGCG 4 cut(s) 148, 277, 358, 1691
BstH2I RGCGCY 1 cut(s) 51
BstHHI GCGC 6 cut(s) 50, 150, 279, 570, 1275, 1390
BstKTI GATC 3 cut(s) 88, 563, 1531
BstMAI GTCTC 3 cut(s) 300, 729, 1475
BstMBI GATC 3 cut(s) 85, 560, 1528
BstNI CCWGG 4 cut(s) 401, 407, 450, 1731
BstNSI RCATGY 1 cut(s) 1675
BstPAI GACNNNNGTC 1 cut(s) 833
BstSCI CCNGG 4 cut(s) 399, 405, 448, 1729
BstSFI CTRYAG 1 cut(s) 1700
BstUI CGCG 4 cut(s) 148, 277, 358, 1691
BstV1I GCAGC 7 cut(s) 435, 476, 619, 665, 829, 1635, 1686
BstV2I GAAGAC 4 cut(s) 113, 207, 1640, 1692
BstXI CCANNNNNNTGG 2 cut(s) 628, 1009
BstZ17I GTATAC 1 cut(s) 1523
Bsu15I ATCGAT 2 cut(s) 712, 1881
BsuI GTATCC 1 cut(s) 1222
BsuRI GGCC 6 cut(s) 329, 384, 447, 1073, 1785, 1855
BsuTUI ATCGAT 2 cut(s) 712, 1881
BtsCI GGATG 6 cut(s) 160, 637, 805, 1265, 1711, 1906
BtsI GCAGTG 2 cut(s) 382, 1107
BtsIMutI CAGTG 7 cut(s) 118, 382, 734, 1107, 1351, 1765, 1895
BveI ACCTGC 2 cut(s) 478, 794
Cac8I GCNNGC 6 cut(s) 327, 373, 382, 901, 905, 1673
CaiI CAGNNNCTG 3 cut(s) 406, 470, 823
CciI TCATGA 1 cut(s) 1093
CfoI GCGC 6 cut(s) 50, 150, 279, 570, 1275, 1390
Cfr10I RCCGGY 2 cut(s) 50, 1655
Cfr13I GGNCC 9 cut(s) 403, 446, 742, 877, 999, 1109, 1136, 1783, 1854
ClaI ATCGAT 2 cut(s) 712, 1881
CseI GACGC 3 cut(s) 109, 896, 1697
Csp6I GTAC 2 cut(s) 33, 1620
CspAI ACCGGT 1 cut(s) 1655
CviAII CATG 8 cut(s) 427, 869, 1094, 1295, 1394, 1493, 1591, 1672
CviQI GTAC 2 cut(s) 33, 1620
DdeI CTNAG 8 cut(s) 138, 297, 321, 336, 345, 726, 1310, 1332
DpnI GATC 3 cut(s) 87, 562, 1530
DpnII GATC 3 cut(s) 85, 560, 1528
DraI TTTAAA 1 cut(s) 981
DraIII CACNNNGTG 1 cut(s) 602
Eam1104I CTCTTC 4 cut(s) 38, 1008, 1299, 1864
EarI CTCTTC 4 cut(s) 38, 1008, 1299, 1864
Eco130I CCWWGG 2 cut(s) 385, 928
Eco32I GATATC 2 cut(s) 61, 1324
Eco47I GGWCC 6 cut(s) 403, 742, 877, 999, 1109, 1136
Eco57I CTGAAG 4 cut(s) 803, 812, 1485, 1704
Eco88I CYCGRG 1 cut(s) 416
EcoNI CCTNNNNNAGG 2 cut(s) 389, 474
EcoO109I RGGNCCY 3 cut(s) 403, 1109, 1854
EcoRI GAATTC 3 cut(s) 257, 270, 439
EcoRII CCWGG 4 cut(s) 399, 405, 448, 1729
EcoRV GATATC 2 cut(s) 61, 1324
EcoT14I CCWWGG 2 cut(s) 385, 928
ErhI CCWWGG 2 cut(s) 385, 928
FaeI CATG 8 cut(s) 430, 872, 1097, 1298, 1397, 1496, 1594, 1675
FalI AAGNNNNNCTT 4 cut(s) 86, 118, 1293, 1325
FaqI GGGAC 1 cut(s) 1122
FatI CATG 8 cut(s) 426, 868, 1093, 1294, 1393, 1492, 1590, 1671
FauI CCCGC 1 cut(s) 292
FbaI TGATCA 1 cut(s) 85
FblI GTMKAC 1 cut(s) 1522
FokI GGATG 6 cut(s) 167, 644, 812, 1252, 1718, 1913
FspBI CTAG 3 cut(s) 1058, 1133, 1572
GlaI GCGC 6 cut(s) 49, 149, 278, 569, 1274, 1389
GsaI CCCAGC 1 cut(s) 1408
HaeII RGCGCY 1 cut(s) 51
HaeIII GGCC 6 cut(s) 329, 384, 447, 1073, 1785, 1855
HapII CCGG 3 cut(s) 51, 444, 1656
HgaI GACGC 3 cut(s) 109, 896, 1697
HhaI GCGC 6 cut(s) 50, 150, 279, 570, 1275, 1390
Hin1I GRCGYC 1 cut(s) 120
Hin1II CATG 8 cut(s) 430, 872, 1097, 1298, 1397, 1496, 1594, 1675
Hin6I GCGC 6 cut(s) 48, 148, 277, 568, 1273, 1388
HinP1I GCGC 6 cut(s) 48, 148, 277, 568, 1273, 1388
HindIII AAGCTT 3 cut(s) 100, 934, 1028
HinfI GANTC 8 cut(s) 142, 211, 245, 722, 1471, 1726, 1744, 1923
HpaII CCGG 3 cut(s) 51, 444, 1656
HphI GGTGA 6 cut(s) 361, 701, 932, 1030, 1538, 1590
Hpy166II GTNNAC 6 cut(s) 843, 1045, 1523, 1611, 1711, 1918
Hpy188I TCNGA 8 cut(s) 141, 193, 216, 250, 300, 346, 783, 792
Hpy188III TCNNGA 7 cut(s) 418, 911, 945, 1058, 1094, 1630, 1748
Hpy8I GTNNAC 6 cut(s) 843, 1045, 1523, 1611, 1711, 1918
Hpy99I CGWCG 1 cut(s) 269
HpyAV CCTTC 2 cut(s) 787, 911
HpyCH4III ACNGT 3 cut(s) 186, 1287, 1760
HpyF3I CTNAG 8 cut(s) 138, 297, 321, 336, 345, 726, 1310, 1332
Hsp92I GRCGYC 1 cut(s) 120
Hsp92II CATG 8 cut(s) 430, 872, 1097, 1298, 1397, 1496, 1594, 1675
HspAI GCGC 6 cut(s) 48, 148, 277, 568, 1273, 1388
Ksp22I TGATCA 1 cut(s) 85
Kzo9I GATC 3 cut(s) 85, 560, 1528
LguI GCTCTTC 2 cut(s) 38, 1299
LmnI GCTCC 4 cut(s) 5, 314, 420, 1280
Lsp1109I GCAGC 7 cut(s) 435, 476, 619, 665, 829, 1635, 1686
LweI GCATC 5 cut(s) 704, 790, 804, 1399, 1686
MaeI CTAG 3 cut(s) 1058, 1133, 1572
MaeIII GTNAC 6 cut(s) 121, 920, 1596, 1802, 1829, 1839
MalI GATC 3 cut(s) 87, 562, 1530
MbiI CCGCTC 1 cut(s) 8
MboI GATC 3 cut(s) 85, 560, 1528
MlyI GAGTC 5 cut(s) 136, 254, 731, 1753, 1917
MmeI TCCRAC 1 cut(s) 855
MroXI GAANNNNTTC 1 cut(s) 1470
MseI TTAA 7 cut(s) 228, 525, 641, 950, 972, 980, 1299
MslI CAYNNNNRTG 1 cut(s) 155
MspA1I CMGCKG 3 cut(s) 656, 1142, 1648
MspI CCGG 3 cut(s) 51, 444, 1656
MspR9I CCNGG 4 cut(s) 401, 407, 450, 1731
MvaI CCWGG 4 cut(s) 401, 407, 450, 1731
MvnI CGCG 4 cut(s) 148, 277, 358, 1691
NdeII GATC 3 cut(s) 85, 560, 1528
NlaIII CATG 8 cut(s) 430, 872, 1097, 1298, 1397, 1496, 1594, 1675
NlaIV GGNNCC 3 cut(s) 532, 1110, 1432
NmuCI GTSAC 4 cut(s) 121, 920, 1596, 1829
NspI RCATGY 1 cut(s) 1675
NspV TTCGAA 1 cut(s) 1474
OliI CACNNNNGTG 1 cut(s) 155
PaeI GCATGC 1 cut(s) 1675
PagI TCATGA 1 cut(s) 1093
PaqCI CACCTGC 1 cut(s) 478
PciSI GCTCTTC 2 cut(s) 38, 1299
PcsI WCGNNNNNNNCGW 1 cut(s) 32
PdmI GAANNNNTTC 1 cut(s) 1470
PfeI GAWTC 3 cut(s) 211, 1471, 1726
PflMI CCANNNNNTGG 1 cut(s) 406
PfoI TCCNGGA 1 cut(s) 405
PinAI ACCGGT 1 cut(s) 1655
PleI GAGTC 5 cut(s) 136, 253, 730, 1752, 1917
PpsI GAGTC 5 cut(s) 136, 253, 730, 1752, 1917
PpuMI RGGWCCY 2 cut(s) 403, 1109
PshAI GACNNNNGTC 1 cut(s) 833
PshBI ATTAAT 1 cut(s) 228
Psp5II RGGWCCY 2 cut(s) 403, 1109
Psp6I CCWGG 4 cut(s) 399, 405, 448, 1729
PspFI CCCAGC 1 cut(s) 1404
PspGI CCWGG 4 cut(s) 399, 405, 448, 1729
PspN4I GGNNCC 3 cut(s) 532, 1110, 1432
PspPI GGNCC 9 cut(s) 403, 446, 742, 877, 999, 1109, 1136, 1783, 1854
PspPPI RGGWCCY 2 cut(s) 403, 1109
PstI CTGCAG 1 cut(s) 1704
PstNI CAGNNNCTG 3 cut(s) 406, 470, 823
PvuII CAGCTG 3 cut(s) 656, 1142, 1648
RsaI GTAC 2 cut(s) 34, 1621
RsaNI GTAC 2 cut(s) 33, 1620
RseI CAYNNNNRTG 1 cut(s) 155
SapI GCTCTTC 2 cut(s) 38, 1299
SaqAI TTAA 7 cut(s) 228, 525, 641, 950, 972, 980, 1299
Sau3AI GATC 3 cut(s) 85, 560, 1528
Sau96I GGNCC 9 cut(s) 403, 446, 742, 877, 999, 1109, 1136, 1783, 1854
SchI GAGTC 5 cut(s) 136, 254, 731, 1753, 1917
ScrFI CCNGG 4 cut(s) 401, 407, 450, 1731
SfaNI GCATC 5 cut(s) 704, 790, 804, 1399, 1686
SfcI CTRYAG 1 cut(s) 1700
SfuI TTCGAA 1 cut(s) 1474
SgrAI CRCCGGYG 1 cut(s) 1655
SinI GGWCC 6 cut(s) 403, 742, 877, 999, 1109, 1136
SmiMI CAYNNNNRTG 1 cut(s) 155
SmlI CTYRAG 1 cut(s) 937
SmoI CTYRAG 1 cut(s) 937
SphI GCATGC 1 cut(s) 1675
SsiI CCGC 9 cut(s) 8, 117, 146, 275, 285, 330, 462, 1250, 1691
SspMI CTAG 3 cut(s) 1058, 1133, 1572
StyD4I CCNGG 4 cut(s) 399, 405, 448, 1729
StyI CCWWGG 2 cut(s) 385, 928
TaaI ACNGT 3 cut(s) 186, 1287, 1760
TaqI TCGA 6 cut(s) 26, 712, 809, 1474, 1527, 1881
TaqII GACCGA 2 cut(s) 759, 894
TatI WGTACW 1 cut(s) 1619
TauI GCSGC 3 cut(s) 120, 332, 1252
TfiI GAWTC 3 cut(s) 211, 1471, 1726
Tru1I TTAA 7 cut(s) 228, 525, 641, 950, 972, 980, 1299
Tru9I TTAA 7 cut(s) 228, 525, 641, 950, 972, 980, 1299
TscAI CASTG 7 cut(s) 118, 382, 734, 1107, 1358, 1765, 1902
TseFI GTSAC 4 cut(s) 121, 920, 1596, 1829
TseI GCWGC 7 cut(s) 423, 464, 607, 653, 817, 1648, 1699
Tsp45I GTSAC 4 cut(s) 121, 920, 1596, 1829
TspDTI ATGAA 7 cut(s) 270, 498, 648, 857, 1110, 1283, 1607
TspGWI ACGGA 6 cut(s) 117, 167, 282, 1599, 1738, 1935
TspRI CASTG 7 cut(s) 118, 382, 734, 1107, 1358, 1765, 1902
Van91I CCANNNNNTGG 1 cut(s) 406
VpaK11BI GGWCC 6 cut(s) 403, 742, 877, 999, 1109, 1136
VspI ATTAAT 1 cut(s) 228
XagI CCTNNNNNAGG 2 cut(s) 389, 474
XapI RAATTY 5 cut(s) 257, 270, 433, 439, 678
XbaI TCTAGA 1 cut(s) 1057
XceI RCATGY 1 cut(s) 1675
XmiI GTMKAC 1 cut(s) 1522
XmnI GAANNNNTTC 1 cut(s) 1470
XspI CTAG 3 cut(s) 1058, 1133, 1572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.