pycom15g13650

26S proteasome non-ATPase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0011000
Physical Location & Seq
Forward (+)
9260315 .. 9262068
1754 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 660 bp
ATGGTGGGAACGAATCTGAAGGCGGAGACGATGGGTTTAATGGAGAAGAGGACCGCCCTGGAGGCGGAGATGAACGCGATCATAGAGCGTCTTACTCAGCCCGGCGGCCCTGGAATCTCCGGCAACCTCCTTGACTCCGAGGGTTTTCCTCGCGAAGACATTGACATCCCAGCTGTGCGAGCAGAAAGGCGTCGTCTTGCTGAATTGCGTAATGATCACAGAGAGATTACCGAGAAACTGAACAGAAATATTGAAGTTCTGCATTCGGCAAGTCTTGCTCCTAAACAATCATCACTCAATGATTCAGATGGCCAAAGCGCATCAGCTGTCAACGATGTTGCATCTGCATCCTCAACCAATGTTCATACTGCCATGGATGTGGATATGATTGTGAGCGTACCCTTTGCATTGGTGGATGAGATAGCTGATGCATCACCAGCAGCAGAGGATGGTTTACAGCTTGGAGATCAGATTGTTAAGTTTGGTAATGTGGAAAATGGTGATAATTTGTTGCAGAAACTTGCTTCTGAGGCTCAAGCTAATCAGGGCCGTGGGATACCAATAATACTTGTAAGGCAAGGTGCTCAAGTCAACTTAACCGTGACACCTAGAACATGGCAAGGCAGGGGTCTACTTGGCTGTCATTTCCGGATCCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PDZ_2

Protein Analysis

220

Amino Acids

23.55

Weight (kDa)

4.96

Isoelectric Point (pI)

42.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nas2_N PF18265 12 - 89 4.6e-23 Nas2 N_terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011000)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G57950 AT5G57950
fragaria_vesca FvH4_1g00940 FvH4_1g00940 FvH4_1g00940
malus_domestica MD02G1007700.v1.1 MD09G1175000.v1.1
prunus_persica Prupe.7G262800_v2.0.a1
pyrus_communis pycom02g00660 pycom15g13650
rosa_chinensis RchiOBHm_Chr2g0085671
rosa_laevigata RLG00000015710
rosa_multiflora Rmu_co8318769.1_g000001 Rmu_sc0007631.1_g000007
rosa_roxburghii Rroxscaffold_2G00155140
rosa_rugosa Rorug01G0462100
rosa_samantha Rh2AG011300 Rh2BG011800 Rh2CG012600
rosa_wichuraiana Rw2G001000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 631
AccII CGCG 2 cut(s) 77, 153
AccIII TCCGGA 1 cut(s) 648
AciI CCGC 4 cut(s) 23, 54, 65, 105
AclWI GGATC 2 cut(s) 646, 659
AcoI YGGCCR 1 cut(s) 310
AcuI CTGAAG 1 cut(s) 38
AcyI GRCGYC 1 cut(s) 190
AfaI GTAC 1 cut(s) 399
AfiI CCNNNNNNNGG 1 cut(s) 64
AgsI TTSAA 1 cut(s) 254
AjnI CCWGG 2 cut(s) 57, 109
AluBI AGCT 5 cut(s) 173, 326, 425, 460, 539
AluI AGCT 5 cut(s) 173, 326, 425, 460, 539
Alw21I GWGCWC 1 cut(s) 586
Alw26I GTCTC 1 cut(s) 20
AlwI GGATC 2 cut(s) 646, 659
Aor13HI TCCGGA 1 cut(s) 648
AoxI GGCC 3 cut(s) 106, 310, 547
ApeKI GCWGC 1 cut(s) 440
AspLEI GCGC 1 cut(s) 320
AspS9I GGNCC 3 cut(s) 51, 107, 547
AsuC2I CCSGG 1 cut(s) 102
AsuHPI GGTGA 2 cut(s) 426, 512
AvaII GGWCC 1 cut(s) 51
BalI TGGCCA 1 cut(s) 312
BamHI GGATCC 1 cut(s) 651
BbsI GAAGAC 1 cut(s) 162
Bbv12I GWGCWC 1 cut(s) 586
BbvI GCAGC 1 cut(s) 452
BccI CCATC 3 cut(s) 25, 302, 443
BceAI ACGGC 1 cut(s) 534
BciT130I CCWGG 2 cut(s) 59, 111
BciVI GTATCC 1 cut(s) 549
BclI TGATCA 1 cut(s) 214
BcnI CCSGG 1 cut(s) 102
BcoDI GTCTC 1 cut(s) 20
BfaI CTAG 1 cut(s) 609
BfuI GTATCC 1 cut(s) 549
BglI GCCNNNNNGGC 1 cut(s) 62
BisI GCNGC 2 cut(s) 106, 441
BlsI GCNGC 2 cut(s) 107, 442
Bme1390I CCNGG 3 cut(s) 59, 102, 111
Bme18I GGWCC 1 cut(s) 51
BmgT120I GGNCC 3 cut(s) 51, 107, 547
BmiI GGNNCC 1 cut(s) 653
BmrFI CCNGG 3 cut(s) 59, 102, 111
BmsI GCATC 5 cut(s) 329, 350, 356, 418, 440
BpiI GAAGAC 1 cut(s) 162
BpmI CTGGAG 1 cut(s) 80
BpuEI CTTGAG 2 cut(s) 519, 570
BpuMI CCSGG 1 cut(s) 102
BsaHI GRCGYC 1 cut(s) 190
BsaJI CCNNGG 5 cut(s) 57, 109, 138, 372, 550
BsaWI WCCGGW 1 cut(s) 648
Bsc4I CCNNNNNNNGG 1 cut(s) 64
BseAI TCCGGA 1 cut(s) 648
BseBI CCWGG 2 cut(s) 59, 111
BseDI CCNNGG 5 cut(s) 57, 109, 138, 372, 550
BseGI GGATG 5 cut(s) 165, 347, 382, 421, 454
BseLI CCNNNNNNNGG 1 cut(s) 64
BseMII CTCAG 2 cut(s) 110, 519
BseXI GCAGC 1 cut(s) 452
BseYI CCCAGC 1 cut(s) 169
Bsh1236I CGCG 2 cut(s) 77, 153
BshFI GGCC 3 cut(s) 108, 312, 549
BsiHKAI GWGCWC 1 cut(s) 586
BsiSI CCGG 3 cut(s) 102, 120, 649
BslI CCNNNNNNNGG 1 cut(s) 64
BsmAI GTCTC 1 cut(s) 20
BsmBI CGTCTC 1 cut(s) 20
BsmI GAATGC 1 cut(s) 262
BsnI GGCC 3 cut(s) 108, 312, 549
Bsp1286I GDGCHC 1 cut(s) 586
Bsp13I TCCGGA 1 cut(s) 648
Bsp143I GATC 4 cut(s) 78, 214, 466, 651
Bsp19I CCATGG 1 cut(s) 372
Bsp68I TCGCGA 1 cut(s) 153
BspACI CCGC 4 cut(s) 23, 54, 65, 105
BspANI GGCC 3 cut(s) 108, 312, 549
BspCNI CTCAG 2 cut(s) 109, 520
BspEI TCCGGA 1 cut(s) 648
BspFNI CGCG 2 cut(s) 77, 153
BspLI GGNNCC 1 cut(s) 653
BspPI GGATC 2 cut(s) 646, 659
BssECI CCNNGG 5 cut(s) 57, 109, 138, 372, 550
BssMI GATC 4 cut(s) 78, 214, 466, 651
BssNI GRCGYC 1 cut(s) 190
BssT1I CCWWGG 1 cut(s) 372
Bst2UI CCWGG 2 cut(s) 59, 111
Bst4CI ACNGT 1 cut(s) 601
Bst6I CTCTTC 1 cut(s) 41
BstACI GRCGYC 1 cut(s) 190
BstAPI GCANNNNNTGC 1 cut(s) 275
BstC8I GCNNGC 1 cut(s) 180
BstDEI CTNAG 2 cut(s) 96, 528
BstDSI CCRYGG 2 cut(s) 372, 550
BstF5I GGATG 5 cut(s) 165, 347, 382, 421, 454
BstFNI CGCG 2 cut(s) 77, 153
BstHHI GCGC 1 cut(s) 320
BstKTI GATC 4 cut(s) 81, 217, 469, 654
BstMAI GTCTC 1 cut(s) 20
BstMBI GATC 4 cut(s) 78, 214, 466, 651
BstMWI GCNNNNNNNGC 5 cut(s) 62, 179, 275, 437, 530
BstNI CCWGG 2 cut(s) 59, 111
BstSCI CCNGG 3 cut(s) 57, 100, 109
BstUI CGCG 2 cut(s) 77, 153
BstV1I GCAGC 1 cut(s) 452
BstV2I GAAGAC 1 cut(s) 162
BstX2I RGATCY 1 cut(s) 651
BstXI CCANNNNNNTGG 1 cut(s) 379
BstYI RGATCY 1 cut(s) 651
BsuI GTATCC 1 cut(s) 549
BsuRI GGCC 3 cut(s) 108, 312, 549
BtgI CCRYGG 2 cut(s) 372, 550
BtsCI GGATG 5 cut(s) 165, 347, 382, 421, 454
BtuMI TCGCGA 1 cut(s) 153
Cac8I GCNNGC 1 cut(s) 180
CfoI GCGC 1 cut(s) 320
Cfr13I GGNCC 3 cut(s) 51, 107, 547
CseI GACGC 2 cut(s) 77, 179
Csp6I GTAC 1 cut(s) 398
CviAII CATG 2 cut(s) 373, 615
CviQI GTAC 1 cut(s) 398
DdeI CTNAG 2 cut(s) 96, 528
DpnI GATC 4 cut(s) 80, 216, 468, 653
DpnII GATC 4 cut(s) 78, 214, 466, 651
EaeI YGGCCR 1 cut(s) 310
Eam1104I CTCTTC 1 cut(s) 41
EarI CTCTTC 1 cut(s) 41
EciI GGCGGA 2 cut(s) 38, 80
Eco130I CCWWGG 1 cut(s) 372
Eco47I GGWCC 1 cut(s) 51
Eco57I CTGAAG 1 cut(s) 38
EcoRII CCWGG 2 cut(s) 57, 109
EcoT14I CCWWGG 1 cut(s) 372
EcoT22I ATGCAT 1 cut(s) 433
ErhI CCWWGG 1 cut(s) 372
Esp3I CGTCTC 1 cut(s) 20
FaeI CATG 2 cut(s) 376, 618
FaiI YATR 5 cut(s) 83, 366, 374, 386, 616
FatI CATG 2 cut(s) 372, 614
FbaI TGATCA 1 cut(s) 214
FblI GTMKAC 1 cut(s) 631
Fnu4HI GCNGC 2 cut(s) 106, 441
FokI GGATG 5 cut(s) 152, 334, 389, 428, 461
Fsp4HI GCNGC 2 cut(s) 106, 441
FspBI CTAG 1 cut(s) 609
GlaI GCGC 1 cut(s) 319
GluI GCNGC 2 cut(s) 106, 441
GsaI CCCAGC 1 cut(s) 173
GsuI CTGGAG 1 cut(s) 80
HaeIII GGCC 3 cut(s) 108, 312, 549
HapII CCGG 3 cut(s) 102, 120, 649
HgaI GACGC 2 cut(s) 77, 179
HhaI GCGC 1 cut(s) 320
Hin1I GRCGYC 1 cut(s) 190
Hin1II CATG 2 cut(s) 376, 618
Hin6I GCGC 1 cut(s) 318
HinP1I GCGC 1 cut(s) 318
HincII GTYRAC 2 cut(s) 331, 592
HindII GTYRAC 2 cut(s) 331, 592
HinfI GANTC 4 cut(s) 13, 114, 134, 302
HpaII CCGG 3 cut(s) 102, 120, 649
HphI GGTGA 2 cut(s) 426, 512
Hpy166II GTNNAC 4 cut(s) 331, 455, 592, 632
Hpy188I TCNGA 5 cut(s) 18, 139, 307, 471, 529
Hpy188III TCNNGA 2 cut(s) 152, 649
Hpy8I GTNNAC 4 cut(s) 331, 455, 592, 632
Hpy99I CGWCG 1 cut(s) 195
HpyAV CCTTC 1 cut(s) 13
HpyCH4III ACNGT 1 cut(s) 601
HpyCH4V TGCA 6 cut(s) 262, 341, 347, 407, 431, 514
HpyF10VI GCNNNNNNNGC 5 cut(s) 62, 179, 275, 437, 530
HpyF3I CTNAG 2 cut(s) 96, 528
Hsp92I GRCGYC 1 cut(s) 190
Hsp92II CATG 2 cut(s) 376, 618
HspAI GCGC 1 cut(s) 318
Kpn2I TCCGGA 1 cut(s) 648
Ksp22I TGATCA 1 cut(s) 214
Kzo9I GATC 4 cut(s) 78, 214, 466, 651
LmnI GCTCC 1 cut(s) 283
Lsp1109I GCAGC 1 cut(s) 452
LweI GCATC 5 cut(s) 329, 350, 356, 418, 440
MaeI CTAG 1 cut(s) 609
MaeIII GTNAC 1 cut(s) 601
MalI GATC 4 cut(s) 80, 216, 468, 653
MboI GATC 4 cut(s) 78, 214, 466, 651
MboII GAAGA 2 cut(s) 58, 167
MflI RGATCY 1 cut(s) 651
MhlI GDGCHC 1 cut(s) 586
MlsI TGGCCA 1 cut(s) 312
MluCI AATT 2 cut(s) 203, 505
MluNI TGGCCA 1 cut(s) 312
MlyI GAGTC 1 cut(s) 128
MnlI CCTC 8 cut(s) 42, 55, 133, 137, 159, 361, 439, 523
Mox20I TGGCCA 1 cut(s) 312
Mph1103I ATGCAT 1 cut(s) 433
MroI TCCGGA 1 cut(s) 648
MscI TGGCCA 1 cut(s) 312
MseI TTAA 3 cut(s) 38, 477, 596
MslI CAYNNNNRTG 1 cut(s) 377
Msp20I TGGCCA 1 cut(s) 312
MspA1I CMGCKG 2 cut(s) 173, 326
MspI CCGG 3 cut(s) 102, 120, 649
MspR9I CCNGG 3 cut(s) 59, 102, 111
Mva1269I GAATGC 1 cut(s) 262
MvaI CCWGG 2 cut(s) 59, 111
MvnI CGCG 2 cut(s) 77, 153
MwoI GCNNNNNNNGC 5 cut(s) 62, 179, 275, 437, 530
NciI CCSGG 1 cut(s) 102
NcoI CCATGG 1 cut(s) 372
NdeII GATC 4 cut(s) 78, 214, 466, 651
NlaIII CATG 2 cut(s) 376, 618
NlaIV GGNNCC 1 cut(s) 653
NmuCI GTSAC 1 cut(s) 601
NruI TCGCGA 1 cut(s) 153
NsiI ATGCAT 1 cut(s) 433
PctI GAATGC 1 cut(s) 262
PfeI GAWTC 3 cut(s) 13, 114, 302
PkrI GCNGC 2 cut(s) 107, 442
PleI GAGTC 1 cut(s) 128
PpsI GAGTC 1 cut(s) 128
Psp6I CCWGG 2 cut(s) 57, 109
PspFI CCCAGC 1 cut(s) 169
PspGI CCWGG 2 cut(s) 57, 109
PspN4I GGNNCC 1 cut(s) 653
PspPI GGNCC 3 cut(s) 51, 107, 547
PsuI RGATCY 1 cut(s) 651
PvuII CAGCTG 2 cut(s) 173, 326
RruI TCGCGA 1 cut(s) 153
RsaI GTAC 1 cut(s) 399
RsaNI GTAC 1 cut(s) 398
RseI CAYNNNNRTG 1 cut(s) 377
SaqAI TTAA 3 cut(s) 38, 477, 596
SatI GCNGC 2 cut(s) 106, 441
Sau3AI GATC 4 cut(s) 78, 214, 466, 651
Sau96I GGNCC 3 cut(s) 51, 107, 547
SchI GAGTC 1 cut(s) 128
ScrFI CCNGG 3 cut(s) 59, 102, 111
SduI GDGCHC 1 cut(s) 586
SetI ASST 8 cut(s) 129, 175, 328, 427, 462, 541, 583, 610
SfaNI GCATC 5 cut(s) 329, 350, 356, 418, 440
SinI GGWCC 1 cut(s) 51
SmiMI CAYNNNNRTG 1 cut(s) 377
SmlI CTYRAG 2 cut(s) 534, 585
SmoI CTYRAG 2 cut(s) 534, 585
Sse9I AATT 2 cut(s) 203, 505
SsiI CCGC 4 cut(s) 23, 54, 65, 105
SspI AATATT 1 cut(s) 250
SspMI CTAG 1 cut(s) 609
StyD4I CCNGG 3 cut(s) 57, 100, 109
StyI CCWWGG 1 cut(s) 372
TaaI ACNGT 1 cut(s) 601
TasI AATT 2 cut(s) 203, 505
TauI GCSGC 1 cut(s) 108
TfiI GAWTC 3 cut(s) 13, 114, 302
Tru1I TTAA 3 cut(s) 38, 477, 596
Tru9I TTAA 3 cut(s) 38, 477, 596
TseFI GTSAC 1 cut(s) 601
TseI GCWGC 1 cut(s) 440
Tsp45I GTSAC 1 cut(s) 601
TspDTI ATGAA 2 cut(s) 86, 353
VpaK11BI GGWCC 1 cut(s) 51
XmiI GTMKAC 1 cut(s) 631
XspI CTAG 1 cut(s) 609
Zsp2I ATGCAT 1 cut(s) 433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.