pycom15g16270

Tudor domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0011849
Physical Location & Seq
Forward (+)
11404548 .. 11407332
2785 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1329 bp
ATGGATGGGGGGAGTTCCGGCGCCGCTTCCGACGCTGCAATCGAAACCCTAAGAACCAGAGGATGGTGCTTCGGCGACCTCGAACAAGTGAAGGCGATGATCCTGATCCACTCCGCCTTGTCTGACGACACGCGTACGGTGGTGGATTCGGTGGAATCGGAGCTCACCAACGTGGACCTCAAATCCATCTCATCCAGGTCCTTGCCCGACCCGCATACACTCCGAAAGTCCTCTCACCTGCTCGGCCCCAAAGTCCTCCAGATAGCTTGGGTGAGAGACGTTACCAGAAGTGGTGTGGAGGATTTTACAAGGAATTCTAATAGTCGGCGTCTTTTGAAACTTGGGCTCACAGACGGCTACAGTGAGGTAGCAGCCGTGGAGTACTCTCATATCCCGGCAATCCCCGACGACGTCGTTCCGGGTACTAAGGTCCGTTTGGAAGGTAAAGCTACTATCCGTAGTGGAATAGTATGTTTGAATCCTAAAGTTGTGACTGTGTTAGGAGGTGTTGTTCAATCACTTTACGAAGAATGGCAGATGAATAAAAAATATTCTGGGTTCTCACGTTCATCTTTAAGGCTTTCACAGGAAAGTGATGGTAAAGGCCCCCCTCCATTTGAGAAATTGCAAATTGGGGCAGCTAAAAGTCGGTTTCCTCAGCAGGACAAGTCTTCACATTTCTTGGATGTTAGCTCCAAGATTAGTGGGCCTATTGCCAGAAATACTGTGGTTAGACCAGTTGGAAGGCAGCAGAACCTTCAATTGAAAGCAAATGACGAGGATAAATCTTATAAAAAAAGTGAGATAGATAACACTTGTAAGGCAGATTTCCTTGATGAAAGAACTCAAGACAAACCTAGTAGCTCTGCAACGACAACGAGGCCAAAAGAAGCAATTGAAGCTGTCCCTGTTCAAAACCAAGCAGCTGCACAAAAACTCCTTCAAACAATGAGTCATCAAAACCGGGATGACAGGCATTCAAGAGGTAGACAGTTTAGGGGAAAGGGGAGGCAAGAGGAAGAACCCGTGGTGTTCACTTTGGATGAATGGGAAAAGAGAAAAGCTGGGGCAAAGCCTTCAATAGGAGGTGCCTTTTCAAGTAGCAGTCGTGACGAGGAACTTGCACGGCAGCTCCAAACTCAATTTGATTTGGAAGATTATCATGTACAAAAGGGTCCTCCTGACGTGGATGCAGAGAATATTAAAATGAGCATGTTCAGCTACCAAAAAGACGATGACAGGGATTATGAAGGGAGAGGAAGAGGAGGGAGGGGACGAGGAAGGGGACGGGGGAGGAGTAGGGGGAGAGGAAGAGGAGGATTTCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RMI1_N

Protein Analysis

443

Amino Acids

48.98

Weight (kDa)

9.34

Isoelectric Point (pI)

47.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RMI1_N_C PF08585 104 - 180 2.3e-18 RMI1, N-terminal OB-fold domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011849)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19950 AT5G19950 AT5G19950
fragaria_vesca FvH4_1g04220
malus_domestica MD00G1026600.v1.1 MD02G1038900.v1.1 MD02G1061400.v1.1
prunus_persica Prupe.7G235200_v2.0.a1
pyrus_communis pycom15g16270
rosa_chinensis RchiOBHm_Chr2g0089761
rosa_laevigata RLG00000016053
rosa_multiflora Rmu_sc0000665.1_g000010
rosa_roxburghii Rroxscaffold_2G00151640
rosa_rugosa Rorug02G0003000
rosa_samantha Rh2AG048200 Rh2BG047000 Rh2CG049100 Rh2DG048500
rosa_wichuraiana Rw2G004220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 792
AarI CACCTGC 1 cut(s) 246
AatII GACGTC 1 cut(s) 414
Acc36I ACCTGC 1 cut(s) 246
AccB1I GGYRCC 2 cut(s) 20, 1088
AccB7I CCANNNNNTGG 1 cut(s) 63
AccI GTMKAC 1 cut(s) 988
AccII CGCG 1 cut(s) 133
AciI CCGC 3 cut(s) 24, 114, 212
AclWI GGATC 2 cut(s) 94, 100
AcsI RAATTY 1 cut(s) 313
AcyI GRCGYC 3 cut(s) 21, 328, 411
AfaI GTAC 4 cut(s) 136, 383, 424, 1167
AfiI CCNNNNNNNGG 2 cut(s) 63, 1082
AflIII ACRYGT 1 cut(s) 131
AjiI CACGTC 1 cut(s) 1186
AjnI CCWGG 1 cut(s) 194
AleI CACNNNNGTG 1 cut(s) 170
AloI GAACNNNNNNTCC 2 cut(s) 495, 527
Alw21I GWGCWC 1 cut(s) 165
Alw26I GTCTC 1 cut(s) 270
AlwI GGATC 2 cut(s) 94, 100
AoxI GGCC 4 cut(s) 244, 604, 707, 881
ApeKI GCWGC 7 cut(s) 35, 371, 638, 748, 923, 926, 1129
ApoI RAATTY 1 cut(s) 313
ArsI GACNNNNNNTTYG 2 cut(s) 937, 969
AspLEI GCGC 1 cut(s) 23
AspS9I GGNCC 7 cut(s) 175, 198, 245, 430, 605, 707, 1175
AsuC2I CCSGG 3 cut(s) 395, 420, 965
AsuHPI GGTGA 3 cut(s) 157, 227, 283
AvaII GGWCC 4 cut(s) 175, 198, 430, 1175
BaeI ACNNNNGTAYC 2 cut(s) 414, 447
BanI GGYRCC 2 cut(s) 20, 1088
BanII GRGCYC 2 cut(s) 165, 348
BbsI GAAGAC 1 cut(s) 663
Bbv12I GWGCWC 1 cut(s) 165
BbvCI CCTCAGC 1 cut(s) 657
BbvI GCAGC 7 cut(s) 22, 383, 650, 760, 913, 935, 1141
BccI CCATC 3 cut(s) 57, 194, 590
BceAI ACGGC 3 cut(s) 359, 370, 1142
BcgI CGANNNNNNTGC 2 cut(s) 1103, 1137
BciT130I CCWGG 1 cut(s) 196
BcnI CCSGG 3 cut(s) 395, 420, 965
BcoDI GTCTC 1 cut(s) 270
BfaI CTAG 1 cut(s) 858
BfmI CTRYAG 1 cut(s) 358
BfoI RGCGCY 1 cut(s) 24
BfuAI ACCTGC 1 cut(s) 246
BisI GCNGC 8 cut(s) 24, 36, 372, 639, 749, 924, 927, 1130
BlsI GCNGC 8 cut(s) 25, 37, 373, 640, 750, 925, 928, 1131
BmcAI AGTACT 1 cut(s) 383
Bme1390I CCNGG 4 cut(s) 196, 395, 420, 965
Bme18I GGWCC 4 cut(s) 175, 198, 430, 1175
BmgBI CACGTC 1 cut(s) 1186
BmgT120I GGNCC 7 cut(s) 175, 198, 245, 430, 605, 707, 1175
BmiI GGNNCC 5 cut(s) 22, 247, 607, 1090, 1176
BmrFI CCNGG 4 cut(s) 196, 395, 420, 965
BmsI GCATC 1 cut(s) 1180
BpiI GAAGAC 1 cut(s) 663
BpmI CTGGAG 1 cut(s) 242
Bpu10I CCTNAGC 1 cut(s) 657
BpuEI CTTGAG 1 cut(s) 831
BpuMI CCSGG 3 cut(s) 395, 420, 965
BsaBI GATNNNNATC 1 cut(s) 104
BsaHI GRCGYC 3 cut(s) 21, 328, 411
BsaJI CCNNGG 2 cut(s) 375, 1026
BsaXI ACNNNNNCTCC 6 cut(s) 495, 525, 921, 951, 1116, 1146
Bsc4I CCNNNNNNNGG 2 cut(s) 63, 1082
Bse1I ACTGG 1 cut(s) 737
Bse8I GATNNNNATC 1 cut(s) 104
BseBI CCWGG 1 cut(s) 196
BseDI CCNNGG 2 cut(s) 375, 1026
BseGI GGATG 7 cut(s) 10, 68, 191, 691, 973, 1048, 1195
BseJI GATNNNNATC 1 cut(s) 104
BseLI CCNNNNNNNGG 2 cut(s) 63, 1082
BseMII CTCAG 1 cut(s) 671
BseNI ACTGG 1 cut(s) 737
BseRI GAGGAG 3 cut(s) 1278, 1309, 1329
BseXI GCAGC 7 cut(s) 22, 383, 650, 760, 913, 935, 1141
BseYI CCCAGC 1 cut(s) 1064
BsgI GTGCAG 1 cut(s) 912
Bsh1236I CGCG 1 cut(s) 133
BshFI GGCC 4 cut(s) 246, 606, 709, 883
BshNI GGYRCC 2 cut(s) 20, 1088
BsiHKAI GWGCWC 1 cut(s) 165
BsiSI CCGG 4 cut(s) 18, 395, 419, 964
BsiWI CGTACG 1 cut(s) 134
BslFI GGGAC 3 cut(s) 890, 1287, 1299
BslI CCNNNNNNNGG 2 cut(s) 63, 1082
BsmAI GTCTC 1 cut(s) 270
BsmBI CGTCTC 1 cut(s) 270
BsmFI GGGAC 3 cut(s) 890, 1287, 1299
BsmI GAATGC 1 cut(s) 976
BsnI GGCC 4 cut(s) 246, 606, 709, 883
Bsp1286I GDGCHC 2 cut(s) 165, 348
Bsp1407I TGTACA 1 cut(s) 1165
Bsp143I GATC 2 cut(s) 99, 105
BspACI CCGC 3 cut(s) 24, 114, 212
BspANI GGCC 4 cut(s) 246, 606, 709, 883
BspCNI CTCAG 1 cut(s) 670
BspFNI CGCG 1 cut(s) 133
BspLI GGNNCC 5 cut(s) 22, 247, 607, 1090, 1176
BspMI ACCTGC 1 cut(s) 246
BspPI GGATC 2 cut(s) 94, 100
BspT107I GGYRCC 2 cut(s) 20, 1088
BsrGI TGTACA 1 cut(s) 1165
BsrI ACTGG 1 cut(s) 737
BssECI CCNNGG 2 cut(s) 375, 1026
BssMI GATC 2 cut(s) 99, 105
BssNI GRCGYC 3 cut(s) 21, 328, 411
Bst2UI CCWGG 1 cut(s) 196
Bst4CI ACNGT 5 cut(s) 139, 362, 496, 727, 993
Bst6I CTCTTC 2 cut(s) 1255, 1306
BstACI GRCGYC 3 cut(s) 21, 328, 411
BstAUI TGTACA 1 cut(s) 1165
BstDEI CTNAG 3 cut(s) 50, 426, 657
BstDSI CCRYGG 2 cut(s) 375, 1026
BstENI CCTNNNNNAGG 1 cut(s) 1080
BstF5I GGATG 7 cut(s) 10, 68, 191, 691, 973, 1048, 1195
BstFNI CGCG 1 cut(s) 133
BstH2I RGCGCY 1 cut(s) 24
BstHHI GCGC 1 cut(s) 23
BstKTI GATC 2 cut(s) 102, 108
BstMAI GTCTC 1 cut(s) 270
BstMBI GATC 2 cut(s) 99, 105
BstMWI GCNNNNNNNGC 4 cut(s) 32, 211, 899, 1218
BstNI CCWGG 1 cut(s) 196
BstNSI RCATGY 1 cut(s) 1216
BstSCI CCNGG 4 cut(s) 194, 393, 418, 963
BstSFI CTRYAG 1 cut(s) 358
BstUI CGCG 1 cut(s) 133
BstV1I GCAGC 7 cut(s) 22, 383, 650, 760, 913, 935, 1141
BstV2I GAAGAC 1 cut(s) 663
BsuRI GGCC 4 cut(s) 246, 606, 709, 883
BtgI CCRYGG 2 cut(s) 375, 1026
BtgZI GCGATG 1 cut(s) 110
BtrI CACGTC 1 cut(s) 1186
BtsCI GGATG 7 cut(s) 10, 68, 191, 691, 973, 1048, 1195
BtsIMutI CAGTG 1 cut(s) 367
BveI ACCTGC 1 cut(s) 246
CfoI GCGC 1 cut(s) 23
Cfr13I GGNCC 7 cut(s) 175, 198, 245, 430, 605, 707, 1175
CseI GACGC 2 cut(s) 41, 317
Csp6I GTAC 4 cut(s) 135, 382, 423, 1166
CspCI CAANNNNNGTGG 2 cut(s) 685, 720
CviAII CATG 2 cut(s) 1163, 1213
CviQI GTAC 4 cut(s) 135, 382, 423, 1166
DdeI CTNAG 3 cut(s) 50, 426, 657
DinI GGCGCC 1 cut(s) 22
DpnI GATC 2 cut(s) 101, 107
DpnII GATC 2 cut(s) 99, 105
Eam1104I CTCTTC 2 cut(s) 1255, 1306
EarI CTCTTC 2 cut(s) 1255, 1306
EciI GGCGGA 1 cut(s) 103
Ecl136II GAGCTC 1 cut(s) 163
Eco24I GRGCYC 2 cut(s) 165, 348
Eco47I GGWCC 4 cut(s) 175, 198, 430, 1175
Eco53kI GAGCTC 1 cut(s) 163
EcoICRI GAGCTC 1 cut(s) 163
EcoNI CCTNNNNNAGG 1 cut(s) 1080
EcoO109I RGGNCCY 3 cut(s) 198, 605, 1175
EcoRI GAATTC 1 cut(s) 313
EcoRII CCWGG 1 cut(s) 194
EcoT38I GRGCYC 2 cut(s) 165, 348
EgeI GGCGCC 1 cut(s) 22
EheI GGCGCC 1 cut(s) 22
Esp3I CGTCTC 1 cut(s) 270
FaeI CATG 2 cut(s) 1166, 1216
FaiI YATR 7 cut(s) 216, 390, 472, 792, 1164, 1214, 1248
FaqI GGGAC 3 cut(s) 890, 1287, 1299
FatI CATG 2 cut(s) 1162, 1212
FauI CCCGC 1 cut(s) 219
FblI GTMKAC 1 cut(s) 988
Fnu4HI GCNGC 8 cut(s) 24, 36, 372, 639, 749, 924, 927, 1130
FokI GGATG 7 cut(s) 17, 75, 178, 698, 980, 1055, 1202
FriOI GRGCYC 2 cut(s) 165, 348
Fsp4HI GCNGC 8 cut(s) 24, 36, 372, 639, 749, 924, 927, 1130
FspBI CTAG 1 cut(s) 858
GlaI GCGC 1 cut(s) 22
GluI GCNGC 8 cut(s) 24, 36, 372, 639, 749, 924, 927, 1130
GsaI CCCAGC 1 cut(s) 1068
GsuI CTGGAG 1 cut(s) 242
HaeII RGCGCY 1 cut(s) 24
HaeIII GGCC 4 cut(s) 246, 606, 709, 883
HapII CCGG 4 cut(s) 18, 395, 419, 964
HgaI GACGC 2 cut(s) 41, 317
HhaI GCGC 1 cut(s) 23
Hin1I GRCGYC 3 cut(s) 21, 328, 411
Hin1II CATG 2 cut(s) 1166, 1216
Hin6I GCGC 1 cut(s) 21
HinP1I GCGC 1 cut(s) 21
HinfI GANTC 4 cut(s) 146, 155, 478, 952
HpaII CCGG 4 cut(s) 18, 395, 419, 964
HphI GGTGA 3 cut(s) 157, 227, 283
Hpy166II GTNNAC 3 cut(s) 175, 989, 1035
Hpy188I TCNGA 4 cut(s) 31, 124, 160, 224
Hpy188III TCNNGA 6 cut(s) 103, 259, 848, 981, 1109, 1181
Hpy8I GTNNAC 3 cut(s) 175, 989, 1035
Hpy99I CGWCG 4 cut(s) 35, 410, 413, 416
HpyAV CCTTC 8 cut(s) 85, 434, 738, 767, 950, 1086, 1244, 1275
HpyCH4III ACNGT 5 cut(s) 139, 362, 496, 727, 993
HpyCH4IV ACGT 5 cut(s) 171, 279, 411, 565, 1185
HpyCH4V TGCA 6 cut(s) 38, 628, 869, 929, 1124, 1193
HpyF10VI GCNNNNNNNGC 4 cut(s) 32, 211, 899, 1218
HpyF3I CTNAG 3 cut(s) 50, 426, 657
HpySE526I ACGT 5 cut(s) 171, 279, 411, 565, 1185
Hsp92I GRCGYC 3 cut(s) 21, 328, 411
Hsp92II CATG 2 cut(s) 1166, 1216
HspAI GCGC 1 cut(s) 21
KasI GGCGCC 1 cut(s) 20
Kzo9I GATC 2 cut(s) 99, 105
LmnI GCTCC 3 cut(s) 160, 698, 1137
Lsp1109I GCAGC 7 cut(s) 22, 383, 650, 760, 913, 935, 1141
LweI GCATC 1 cut(s) 1180
MaeI CTAG 1 cut(s) 858
MaeII ACGT 5 cut(s) 171, 279, 411, 565, 1185
MaeIII GTNAC 3 cut(s) 280, 490, 1109
MalI GATC 2 cut(s) 101, 107
MboI GATC 2 cut(s) 99, 105
MboII GAAGA 6 cut(s) 539, 663, 1031, 1166, 1272, 1323
MfeI CAATTG 2 cut(s) 761, 894
MhlI GDGCHC 2 cut(s) 165, 348
MluCI AATT 6 cut(s) 313, 623, 630, 761, 894, 1142
MluI ACGCGT 1 cut(s) 131
Mly113I GGCGCC 1 cut(s) 21
MlyI GAGTC 1 cut(s) 961
MmeI TCCRAC 2 cut(s) 54, 721
MseI TTAA 2 cut(s) 575, 1203
MslI CAYNNNNRTG 1 cut(s) 170
MspA1I CMGCKG 1 cut(s) 926
MspI CCGG 4 cut(s) 18, 395, 419, 964
MspR9I CCNGG 4 cut(s) 196, 395, 420, 965
MunI CAATTG 2 cut(s) 761, 894
Mva1269I GAATGC 1 cut(s) 976
MvaI CCWGG 1 cut(s) 196
MvnI CGCG 1 cut(s) 133
MwoI GCNNNNNNNGC 4 cut(s) 32, 211, 899, 1218
NarI GGCGCC 1 cut(s) 21
NciI CCSGG 3 cut(s) 395, 420, 965
NdeII GATC 2 cut(s) 99, 105
NlaIII CATG 2 cut(s) 1166, 1216
NlaIV GGNNCC 5 cut(s) 22, 247, 607, 1090, 1176
NmeAIII GCCGAG 1 cut(s) 222
NmuCI GTSAC 2 cut(s) 490, 1109
NspI RCATGY 1 cut(s) 1216
OliI CACNNNNGTG 1 cut(s) 170
PaqCI CACCTGC 1 cut(s) 246
PcsI WCGNNNNNNNCGW 2 cut(s) 39, 78
PctI GAATGC 1 cut(s) 976
PfeI GAWTC 3 cut(s) 146, 155, 478
Pfl23II CGTACG 1 cut(s) 134
PflFI GACNNNGTC 1 cut(s) 410
PflMI CCANNNNNTGG 1 cut(s) 63
PkrI GCNGC 8 cut(s) 25, 37, 373, 640, 750, 925, 928, 1131
PleI GAGTC 1 cut(s) 960
PluTI GGCGCC 1 cut(s) 24
PpsI GAGTC 1 cut(s) 960
PpuMI RGGWCCY 2 cut(s) 198, 1175
PsiI TTATAA 1 cut(s) 792
Psp124BI GAGCTC 1 cut(s) 165
Psp5II RGGWCCY 2 cut(s) 198, 1175
Psp6I CCWGG 1 cut(s) 194
PspFI CCCAGC 1 cut(s) 1064
PspGI CCWGG 1 cut(s) 194
PspLI CGTACG 1 cut(s) 134
PspN4I GGNNCC 5 cut(s) 22, 247, 607, 1090, 1176
PspPI GGNCC 7 cut(s) 175, 198, 245, 430, 605, 707, 1175
PspPPI RGGWCCY 2 cut(s) 198, 1175
PsyI GACNNNGTC 1 cut(s) 410
PvuII CAGCTG 1 cut(s) 926
RsaI GTAC 4 cut(s) 136, 383, 424, 1167
RsaNI GTAC 4 cut(s) 135, 382, 423, 1166
RseI CAYNNNNRTG 1 cut(s) 170
SacI GAGCTC 1 cut(s) 165
SaqAI TTAA 2 cut(s) 575, 1203
SatI GCNGC 8 cut(s) 24, 36, 372, 639, 749, 924, 927, 1130
Sau3AI GATC 2 cut(s) 99, 105
Sau96I GGNCC 7 cut(s) 175, 198, 245, 430, 605, 707, 1175
ScaI AGTACT 1 cut(s) 383
SchI GAGTC 1 cut(s) 961
ScrFI CCNGG 4 cut(s) 196, 395, 420, 965
SduI GDGCHC 2 cut(s) 165, 348
SfaNI GCATC 1 cut(s) 1180
SfcI CTRYAG 1 cut(s) 358
SfoI GGCGCC 1 cut(s) 22
SinI GGWCC 4 cut(s) 175, 198, 430, 1175
SmiMI CAYNNNNRTG 1 cut(s) 170
SmlI CTYRAG 1 cut(s) 846
SmoI CTYRAG 1 cut(s) 846
Sse9I AATT 6 cut(s) 313, 623, 630, 761, 894, 1142
SsiI CCGC 3 cut(s) 24, 114, 212
SspDI GGCGCC 1 cut(s) 20
SspI AATATT 2 cut(s) 551, 1201
SspMI CTAG 1 cut(s) 858
SstI GAGCTC 1 cut(s) 165
StyD4I CCNGG 4 cut(s) 194, 393, 418, 963
TaaI ACNGT 5 cut(s) 139, 362, 496, 727, 993
TaiI ACGT 5 cut(s) 174, 282, 414, 568, 1188
TaqI TCGA 2 cut(s) 42, 81
TasI AATT 6 cut(s) 313, 623, 630, 761, 894, 1142
TatI WGTACW 2 cut(s) 381, 1165
TauI GCSGC 1 cut(s) 26
TfiI GAWTC 3 cut(s) 146, 155, 478
Tru1I TTAA 2 cut(s) 575, 1203
Tru9I TTAA 2 cut(s) 575, 1203
TscAI CASTG 1 cut(s) 367
TseFI GTSAC 2 cut(s) 490, 1109
TseI GCWGC 7 cut(s) 35, 371, 638, 748, 923, 926, 1129
Tsp45I GTSAC 2 cut(s) 490, 1109
TspDTI ATGAA 5 cut(s) 554, 558, 852, 1059, 1263
TspGWI ACGGA 2 cut(s) 422, 446
TspRI CASTG 1 cut(s) 367
Tth111I GACNNNGTC 1 cut(s) 410
Van91I CCANNNNNTGG 1 cut(s) 63
VpaK11BI GGWCC 4 cut(s) 175, 198, 430, 1175
XagI CCTNNNNNAGG 1 cut(s) 1080
XapI RAATTY 1 cut(s) 313
XceI RCATGY 1 cut(s) 1216
XcmI CCANNNNNNNNNTGG 2 cut(s) 292, 724
XmiI GTMKAC 1 cut(s) 988
XspI CTAG 1 cut(s) 858
ZraI GACGTC 1 cut(s) 412
ZrmI AGTACT 1 cut(s) 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.