pycom15g18330

Polyamine oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0012410
Physical Location & Seq
Reverse (-)
13023702 .. 13025392
1691 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1557 bp
ATGGTGGCCAAGAAATCACGGATTGTGATAATTGGAGCGGGAATGGCCGGCGTCACGGCGGCGAACAAGCTGTACACTGCTGCAGGGTCTAAGGACTTGTTCGAGCTCGTGGTTGTGGAAGGCGGGGAGAGAATTGGCGGCAGAATCAACACGTCGGACCCGGTTCATAAAATTGCTCAGGAAATCAATGCCCTCGAGTCAGCGCAGCCGTGGGAGTGCATGGACGGGTCCTCCGACGAGCCCACCACCGTCGCGGAAGGTGGGTTGGAGCTGGAACCGGATGTGGTGGACCCTATTTCGTCTCTGTTCAAGAACTTGATGGATTATGCTCAGAGGAAGCAGGTGTTTGATGAAAGTACAGACAGAGAGTGCAATGGTGATTTTGAATACAGTAAGCTTGGAGATGAAGCTTCCAGGATTTTTGCAAGCAATGACGGTGTTGGGAAGCTCAGTGTCGGCTCTTTCTTAAGGCAAGGCCTTGATACGTATTGGGTTTGGAAGAAAAACCGAGATGAGCAGGTCAGTGGCTATACAAATTGGAGCAGAAAGTTGCTGCAAGAGGCCATCTTTGCAATGCATGAGAACATCCAAAGGACTTATACATCAGCCGGTGATTTGTTAACTCTCGATTACAATGCAGAAAGCGAGTACCGGATGTTTCCTGGCGAAGAAATCACCATTGCTAAAGGGTACTTGAGCATTGTTCAATCTCTAGCCTCTGTACTCCCACCTGGGTTGATCCAATTGGGCAAGAAGGTCAGAAAAATTCAATGGCAGCCGGATAATCGCAAGAACAAGGGCTATGAATCCGACACAAGGCCTGTGAAGCTCCATTTTTCTGATGGGTCAGTTTTGTTAGCTGATCATGTTATTGTTACAGTTTCTTTGGGGGTGCTCAAAGCTTCGATTCGCCAAGATTCTGGTCTGTTCAATCCACCTCTGCCTCGTTTCAAAACCGAGGCGATTTCGAGGCTTGGATTTGGCGTTGTTAACAAGCTGTTTCTGCAGCTGGGTTCTAATCATGCTCCTCCGAAGTCCCAAGATTTCAGCAAGTTTCCATTTTTACAAATGGTTTTCCACAGAGCAGACTCAGAGCTCAGGAATAAAAATCGATCCCGGGAAGAAGCACGTGCGCTAGAAGCACTTTCAGATGAAGAGATTATCAACGGGGTTTCACAAACGGTGTCCAGCTTCCTATCACAAAACTCACATTCCCATGAATTGTGCAATGGGAATGGGAATCCGGAGGAGAACTCCGAAGTGAAATTTTCCCAGGTTTTGAAGAGTCAATGGGGTAGTGATCCACTGTTTCTGGGATCATACTCGTATGTTGCAGATGGATCAAGCGGTGAGGATTTGGACAGCATGGCAGAGCCATTGCCATCTGATGATTCTGGTAGTGCTGCTAGTTCTTCACCTCCACTGCAGATTCTGTTTGCAGGGGAAGCTACACACAGAACCCATTATTCCACAACCCATGGAGCTTACTGTAGTGGCCTTAGGGAAGCCAGTAGGCTTCTCCAACATTACCAACTTGGTGTTTGGAACAATTATTAG

Protein Analysis

519

Amino Acids

57.11

Weight (kDa)

5.37

Isoelectric Point (pI)

40.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amino_oxidase PF01593 15 - 507 3e-46 Flavin containing amine oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012410)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G29720
fragaria_vesca FvH4_7g01430
malus_domestica MD01G1012400.v1.1 MD02G1079400.v1.1 MD15G1206900.v1.1 MD16G1255500.v1.1
prunus_persica Prupe.7G209800_v2.0.a1
pyrus_communis pycom02g06320 pycom15g18330
rosa_chinensis RchiOBHm_Chr2g0093331
rosa_laevigata RLG00000016391
rosa_multiflora Rmu_sc0005227.1_g000004
rosa_roxburghii Rroxscaffold_2G00148180
rosa_rugosa Rorug02G0034900
rosa_samantha Rh2AG081000 Rh2BG082100 Rh2CG084300 Rh2DG080000
rosa_wichuraiana Rw2G006570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 331
Acc36I ACCTGC 2 cut(s) 331, 508
AccBSI CCGCTC 1 cut(s) 38
AccII CGCG 1 cut(s) 254
AccIII TCCGGA 1 cut(s) 1243
AciI CCGC 6 cut(s) 38, 59, 123, 138, 254, 1347
AclWI GGATC 5 cut(s) 733, 1107, 1295, 1324, 1348
AcoI YGGCCR 2 cut(s) 6, 45
AcsI RAATTY 2 cut(s) 765, 1265
AcvI CACGTG 1 cut(s) 1130
AcyI GRCGYC 1 cut(s) 51
AfaI GTAC 5 cut(s) 74, 358, 650, 692, 723
AfiI CCNNNNNNNGG 1 cut(s) 816
AflII CTTAAG 1 cut(s) 466
AflIII ACRYGT 1 cut(s) 150
AgsI TTSAA 7 cut(s) 310, 386, 707, 770, 931, 952, 1282
AjiI CACGTC 1 cut(s) 153
AjnI CCWGG 4 cut(s) 413, 661, 730, 1272
Alw21I GWGCWC 3 cut(s) 108, 897, 1098
Alw26I GTCTC 1 cut(s) 306
AlwI GGATC 5 cut(s) 733, 1107, 1295, 1324, 1348
AlwNI CAGNNNCTG 1 cut(s) 1432
Ama87I CYCGRG 2 cut(s) 194, 1116
Aor13HI TCCGGA 1 cut(s) 1243
AoxI GGCC 6 cut(s) 6, 45, 475, 561, 818, 1495
ApeKI GCWGC 6 cut(s) 80, 205, 553, 775, 1006, 1403
ApoI RAATTY 2 cut(s) 765, 1265
AspLEI GCGC 2 cut(s) 205, 1135
AspS9I GGNCC 3 cut(s) 157, 228, 289
AsuC2I CCSGG 3 cut(s) 161, 1117, 1118
AsuHPI GGTGA 5 cut(s) 389, 623, 667, 1361, 1407
AvaI CYCGRG 2 cut(s) 194, 1116
AvaII GGWCC 3 cut(s) 157, 228, 289
AxyI CCTNAGG 1 cut(s) 1499
BalI TGGCCA 1 cut(s) 8
BanII GRGCYC 3 cut(s) 108, 243, 1098
BauI CACGAG 1 cut(s) 107
BbrPI CACGTG 1 cut(s) 1130
Bbv12I GWGCWC 3 cut(s) 108, 897, 1098
BbvI GCAGC 6 cut(s) 67, 217, 540, 787, 1018, 1390
BccI CCATC 5 cut(s) 313, 572, 836, 1331, 1390
BceAI ACGGC 2 cut(s) 72, 193
BcgI CGANNNNNNTGC 2 cut(s) 617, 651
BciT130I CCWGG 4 cut(s) 415, 663, 732, 1274
BclI TGATCA 1 cut(s) 862
BcnI CCSGG 3 cut(s) 161, 1117, 1118
BcoDI GTCTC 1 cut(s) 306
BfaI CTAG 3 cut(s) 713, 1136, 1407
BfmI CTRYAG 4 cut(s) 81, 1004, 1424, 1489
BfrI CTTAAG 1 cut(s) 466
BfuAI ACCTGC 2 cut(s) 331, 508
BisI GCNGC 8 cut(s) 60, 81, 139, 206, 554, 776, 1007, 1404
BlsI GCNGC 8 cut(s) 61, 82, 140, 207, 555, 777, 1008, 1405
Bme1390I CCNGG 7 cut(s) 161, 415, 663, 732, 1117, 1118, 1274
Bme18I GGWCC 3 cut(s) 157, 228, 289
BmeT110I CYCGRG 2 cut(s) 194, 1116
BmgBI CACGTC 1 cut(s) 153
BmgT120I GGNCC 3 cut(s) 157, 228, 289
BmiI GGNNCC 4 cut(s) 159, 229, 276, 291
BmrFI CCNGG 7 cut(s) 161, 415, 663, 732, 1117, 1118, 1274
BplI GAGNNNNNCTC 2 cut(s) 1238, 1270
Bpu10I CCTNAGC 2 cut(s) 177, 1097
BpuEI CTTGAG 1 cut(s) 715
BpuMI CCSGG 3 cut(s) 161, 1117, 1118
Bsa29I ATCGAT 1 cut(s) 1111
BsaAI YACGTR 2 cut(s) 486, 1130
BsaHI GRCGYC 1 cut(s) 51
BsaJI CCNNGG 6 cut(s) 209, 731, 957, 1116, 1272, 1477
BsaWI WCCGGW 3 cut(s) 277, 651, 1243
BsaXI ACNNNNNCTCC 2 cut(s) 215, 245
Bsc4I CCNNNNNNNGG 1 cut(s) 816
Bse118I RCCGGY 2 cut(s) 47, 608
Bse1I ACTGG 1 cut(s) 1509
Bse21I CCTNAGG 1 cut(s) 1499
Bse3DI GCAATG 6 cut(s) 379, 436, 579, 678, 1234, 1376
BseAI TCCGGA 1 cut(s) 1243
BseBI CCWGG 4 cut(s) 415, 663, 732, 1274
BseCI ATCGAT 1 cut(s) 1111
BseDI CCNNGG 6 cut(s) 209, 731, 957, 1116, 1272, 1477
BseGI GGATG 3 cut(s) 286, 585, 660
BseLI CCNNNNNNNGG 1 cut(s) 816
BseMI GCAATG 6 cut(s) 379, 436, 579, 678, 1234, 1376
BseMII CTCAG 5 cut(s) 191, 344, 463, 1104, 1111
BseNI ACTGG 1 cut(s) 1509
BseRI GAGGAG 2 cut(s) 1017, 1262
BseXI GCAGC 6 cut(s) 67, 217, 540, 787, 1018, 1390
BseYI CCCAGC 1 cut(s) 1009
Bsh1236I CGCG 1 cut(s) 254
BshFI GGCC 6 cut(s) 8, 47, 477, 563, 820, 1497
BshVI ATCGAT 1 cut(s) 1111
BsiHKAI GWGCWC 3 cut(s) 108, 897, 1098
BsiHKCI CYCGRG 2 cut(s) 194, 1116
BsiSI CCGG 8 cut(s) 48, 161, 278, 609, 652, 779, 1117, 1244
BslFI GGGAC 1 cut(s) 1021
BslI CCNNNNNNNGG 1 cut(s) 816
BsmAI GTCTC 1 cut(s) 306
BsmBI CGTCTC 1 cut(s) 306
BsmFI GGGAC 1 cut(s) 1021
BsnI GGCC 6 cut(s) 8, 47, 477, 563, 820, 1497
BsoBI CYCGRG 2 cut(s) 194, 1116
Bsp1286I GDGCHC 4 cut(s) 108, 243, 897, 1098
Bsp13I TCCGGA 1 cut(s) 1243
Bsp1407I TGTACA 1 cut(s) 72
Bsp143I GATC 6 cut(s) 738, 862, 1112, 1300, 1316, 1340
Bsp19I CCATGG 1 cut(s) 1477
BspACI CCGC 6 cut(s) 38, 59, 123, 138, 254, 1347
BspANI GGCC 6 cut(s) 8, 47, 477, 563, 820, 1497
BspCNI CTCAG 5 cut(s) 190, 343, 462, 1103, 1110
BspDI ATCGAT 1 cut(s) 1111
BspEI TCCGGA 1 cut(s) 1243
BspFNI CGCG 1 cut(s) 254
BspLI GGNNCC 4 cut(s) 159, 229, 276, 291
BspMAI CTGCAG 3 cut(s) 85, 1008, 1428
BspMI ACCTGC 2 cut(s) 331, 508
BspPI GGATC 5 cut(s) 733, 1107, 1295, 1324, 1348
BspTI CTTAAG 1 cut(s) 466
BsrBI CCGCTC 1 cut(s) 38
BsrDI GCAATG 6 cut(s) 379, 436, 579, 678, 1234, 1376
BsrFI RCCGGY 2 cut(s) 47, 608
BsrGI TGTACA 1 cut(s) 72
BsrI ACTGG 1 cut(s) 1509
BssAI RCCGGY 2 cut(s) 47, 608
BssECI CCNNGG 6 cut(s) 209, 731, 957, 1116, 1272, 1477
BssMI GATC 6 cut(s) 738, 862, 1112, 1300, 1316, 1340
BssNI GRCGYC 1 cut(s) 51
BssSI CACGAG 1 cut(s) 107
BssT1I CCWWGG 1 cut(s) 1477
Bst2BI CACGAG 1 cut(s) 107
Bst2UI CCWGG 4 cut(s) 415, 663, 732, 1274
Bst4CI ACNGT 7 cut(s) 250, 392, 437, 880, 1183, 1308, 1490
Bst6I CTCTTC 2 cut(s) 1149, 1277
BstACI GRCGYC 1 cut(s) 51
BstAFI CTTAAG 1 cut(s) 466
BstAUI TGTACA 1 cut(s) 72
BstBAI YACGTR 2 cut(s) 486, 1130
BstC8I GCNNGC 2 cut(s) 49, 427
BstDEI CTNAG 7 cut(s) 90, 177, 330, 449, 1090, 1097, 1499
BstDSI CCRYGG 2 cut(s) 209, 1477
BstF5I GGATG 3 cut(s) 286, 585, 660
BstFNI CGCG 1 cut(s) 254
BstHHI GCGC 2 cut(s) 205, 1135
BstKTI GATC 6 cut(s) 741, 865, 1115, 1303, 1319, 1343
BstMAI GTCTC 1 cut(s) 306
BstMBI GATC 6 cut(s) 738, 862, 1112, 1300, 1316, 1340
BstMWI GCNNNNNNNGC 6 cut(s) 44, 569, 826, 1003, 1139, 1445
BstNI CCWGG 4 cut(s) 415, 663, 732, 1274
BstSCI CCNGG 7 cut(s) 159, 413, 661, 730, 1115, 1116, 1272
BstSFI CTRYAG 4 cut(s) 81, 1004, 1424, 1489
BstSNI TACGTA 1 cut(s) 486
BstUI CGCG 1 cut(s) 254
BstV1I GCAGC 6 cut(s) 67, 217, 540, 787, 1018, 1390
BstXI CCANNNNNNTGG 1 cut(s) 920
Bsu15I ATCGAT 1 cut(s) 1111
Bsu36I CCTNAGG 1 cut(s) 1499
BsuRI GGCC 6 cut(s) 8, 47, 477, 563, 820, 1497
BsuTUI ATCGAT 1 cut(s) 1111
BtgI CCRYGG 2 cut(s) 209, 1477
BtrI CACGTC 1 cut(s) 153
BtsCI GGATG 3 cut(s) 286, 585, 660
BtsI GCAGTG 2 cut(s) 75, 1421
BtsIMutI CAGTG 5 cut(s) 75, 457, 529, 1304, 1421
BveI ACCTGC 2 cut(s) 331, 508
Cac8I GCNNGC 2 cut(s) 49, 427
CaiI CAGNNNCTG 1 cut(s) 1432
CfoI GCGC 2 cut(s) 205, 1135
Cfr10I RCCGGY 2 cut(s) 47, 608
Cfr13I GGNCC 3 cut(s) 157, 228, 289
Cfr9I CCCGGG 1 cut(s) 1116
ClaI ATCGAT 1 cut(s) 1111
CseI GACGC 1 cut(s) 40
Csp6I GTAC 5 cut(s) 73, 357, 649, 691, 722
CspCI CAANNNNNGTGG 2 cut(s) 717, 752
CviAII CATG 7 cut(s) 220, 578, 866, 1022, 1217, 1366, 1478
CviQI GTAC 5 cut(s) 73, 357, 649, 691, 722
DdeI CTNAG 7 cut(s) 90, 177, 330, 449, 1090, 1097, 1499
DpnI GATC 6 cut(s) 740, 864, 1114, 1302, 1318, 1342
DpnII GATC 6 cut(s) 738, 862, 1112, 1300, 1316, 1340
EaeI YGGCCR 2 cut(s) 6, 45
Eam1104I CTCTTC 2 cut(s) 1149, 1277
EarI CTCTTC 2 cut(s) 1149, 1277
Ecl136II GAGCTC 2 cut(s) 106, 1096
Eco105I TACGTA 1 cut(s) 486
Eco130I CCWWGG 1 cut(s) 1477
Eco147I AGGCCT 2 cut(s) 477, 820
Eco24I GRGCYC 3 cut(s) 108, 243, 1098
Eco47I GGWCC 3 cut(s) 157, 228, 289
Eco53kI GAGCTC 2 cut(s) 106, 1096
Eco72I CACGTG 1 cut(s) 1130
Eco81I CCTNAGG 1 cut(s) 1499
Eco88I CYCGRG 2 cut(s) 194, 1116
EcoICRI GAGCTC 2 cut(s) 106, 1096
EcoO109I RGGNCCY 1 cut(s) 228
EcoRII CCWGG 4 cut(s) 413, 661, 730, 1272
EcoT14I CCWWGG 1 cut(s) 1477
EcoT22I ATGCAT 1 cut(s) 579
EcoT38I GRGCYC 3 cut(s) 108, 243, 1098
ErhI CCWWGG 1 cut(s) 1477
Esp3I CGTCTC 1 cut(s) 306
FaeI CATG 7 cut(s) 223, 581, 869, 1025, 1220, 1369, 1481
FaqI GGGAC 1 cut(s) 1021
FatI CATG 7 cut(s) 219, 577, 865, 1021, 1216, 1365, 1477
FauI CCCGC 2 cut(s) 31, 116
FbaI TGATCA 1 cut(s) 862
Fnu4HI GCNGC 8 cut(s) 60, 81, 139, 206, 554, 776, 1007, 1404
FokI GGATG 3 cut(s) 293, 572, 667
FriOI GRGCYC 3 cut(s) 108, 243, 1098
Fsp4HI GCNGC 8 cut(s) 60, 81, 139, 206, 554, 776, 1007, 1404
FspBI CTAG 3 cut(s) 713, 1136, 1407
GlaI GCGC 2 cut(s) 204, 1134
GluI GCNGC 8 cut(s) 60, 81, 139, 206, 554, 776, 1007, 1404
GsaI CCCAGC 1 cut(s) 1013
HaeIII GGCC 6 cut(s) 8, 47, 477, 563, 820, 1497
HapII CCGG 8 cut(s) 48, 161, 278, 609, 652, 779, 1117, 1244
HgaI GACGC 1 cut(s) 40
HhaI GCGC 2 cut(s) 205, 1135
Hin1I GRCGYC 1 cut(s) 51
Hin1II CATG 7 cut(s) 223, 581, 869, 1025, 1220, 1369, 1481
Hin6I GCGC 2 cut(s) 203, 1133
HinP1I GCGC 2 cut(s) 203, 1133
HincII GTYRAC 2 cut(s) 621, 991
HindII GTYRAC 2 cut(s) 621, 991
HindIII AAGCTT 3 cut(s) 395, 408, 900
HpaI GTTAAC 2 cut(s) 621, 991
HpaII CCGG 8 cut(s) 48, 161, 278, 609, 652, 779, 1117, 1244
HphI GGTGA 5 cut(s) 389, 623, 667, 1361, 1407
Hpy166II GTNNAC 4 cut(s) 75, 289, 621, 991
Hpy188III TCNNGA 5 cut(s) 179, 310, 626, 1099, 1244
Hpy8I GTNNAC 4 cut(s) 75, 289, 621, 991
Hpy99I CGWCG 3 cut(s) 157, 239, 254
HpyAV CCTTC 3 cut(s) 113, 251, 748
HpyCH4III ACNGT 7 cut(s) 250, 392, 437, 880, 1183, 1308, 1490
HpyCH4IV ACGT 3 cut(s) 152, 485, 1129
HpyF10VI GCNNNNNNNGC 6 cut(s) 44, 569, 826, 1003, 1139, 1445
HpyF3I CTNAG 7 cut(s) 90, 177, 330, 449, 1090, 1097, 1499
HpySE526I ACGT 3 cut(s) 152, 485, 1129
Hsp92I GRCGYC 1 cut(s) 51
Hsp92II CATG 7 cut(s) 223, 581, 869, 1025, 1220, 1369, 1481
HspAI GCGC 2 cut(s) 203, 1133
Kpn2I TCCGGA 1 cut(s) 1243
KroI GCCGGC 1 cut(s) 47
KroNI GCCGGC 1 cut(s) 49
Ksp22I TGATCA 1 cut(s) 862
KspAI GTTAAC 2 cut(s) 621, 991
Kzo9I GATC 6 cut(s) 738, 862, 1112, 1300, 1316, 1340
LmnI GCTCC 6 cut(s) 35, 268, 540, 834, 1030, 1481
Lsp1109I GCAGC 6 cut(s) 67, 217, 540, 787, 1018, 1390
MaeI CTAG 3 cut(s) 713, 1136, 1407
MaeII ACGT 3 cut(s) 152, 485, 1129
MaeIII GTNAC 2 cut(s) 52, 874
MalI GATC 6 cut(s) 740, 864, 1114, 1302, 1318, 1342
MbiI CCGCTC 1 cut(s) 38
MboI GATC 6 cut(s) 738, 862, 1112, 1300, 1316, 1340
MboII GAAGA 6 cut(s) 511, 680, 1133, 1166, 1294, 1404
MfeI CAATTG 1 cut(s) 743
MhlI GDGCHC 4 cut(s) 108, 243, 897, 1098
MlsI TGGCCA 1 cut(s) 8
MluCI AATT 9 cut(s) 30, 132, 171, 535, 743, 765, 1220, 1265, 1549
MluNI TGGCCA 1 cut(s) 8
MlyI GAGTC 3 cut(s) 206, 1082, 1294
MmeI TCCRAC 5 cut(s) 135, 246, 258, 834, 1546
Mox20I TGGCCA 1 cut(s) 8
Mph1103I ATGCAT 1 cut(s) 579
MroI TCCGGA 1 cut(s) 1243
MroNI GCCGGC 1 cut(s) 47
MscI TGGCCA 1 cut(s) 8
MseI TTAA 3 cut(s) 467, 620, 990
MslI CAYNNNNRTG 1 cut(s) 1215
Msp20I TGGCCA 1 cut(s) 8
MspA1I CMGCKG 1 cut(s) 1009
MspCI CTTAAG 1 cut(s) 466
MspI CCGG 8 cut(s) 48, 161, 278, 609, 652, 779, 1117, 1244
MspR9I CCNGG 7 cut(s) 161, 415, 663, 732, 1117, 1118, 1274
MunI CAATTG 1 cut(s) 743
MvaI CCWGG 4 cut(s) 415, 663, 732, 1274
MvnI CGCG 1 cut(s) 254
MwoI GCNNNNNNNGC 6 cut(s) 44, 569, 826, 1003, 1139, 1445
NaeI GCCGGC 1 cut(s) 49
NciI CCSGG 3 cut(s) 161, 1117, 1118
NcoI CCATGG 1 cut(s) 1477
NdeII GATC 6 cut(s) 738, 862, 1112, 1300, 1316, 1340
NgoMIV GCCGGC 1 cut(s) 47
NlaIII CATG 7 cut(s) 223, 581, 869, 1025, 1220, 1369, 1481
NlaIV GGNNCC 4 cut(s) 159, 229, 276, 291
NmuCI GTSAC 1 cut(s) 52
NsiI ATGCAT 1 cut(s) 579
PaeR7I CTCGAG 1 cut(s) 194
PaqCI CACCTGC 1 cut(s) 331
PceI AGGCCT 2 cut(s) 477, 820
PcsI WCGNNNNNNNCGW 1 cut(s) 231
PdiI GCCGGC 1 cut(s) 49
PfeI GAWTC 7 cut(s) 144, 806, 907, 917, 1240, 1391, 1429
PfoI TCCNGGA 1 cut(s) 413
PkrI GCNGC 8 cut(s) 61, 82, 140, 207, 555, 777, 1008, 1405
PleI GAGTC 3 cut(s) 205, 1082, 1293
PmaCI CACGTG 1 cut(s) 1130
PmlI CACGTG 1 cut(s) 1130
PpsI GAGTC 3 cut(s) 205, 1082, 1293
Ppu21I YACGTR 2 cut(s) 486, 1130
PpuMI RGGWCCY 1 cut(s) 228
Psp124BI GAGCTC 2 cut(s) 108, 1098
Psp5II RGGWCCY 1 cut(s) 228
Psp6I CCWGG 4 cut(s) 413, 661, 730, 1272
PspCI CACGTG 1 cut(s) 1130
PspFI CCCAGC 1 cut(s) 1009
PspGI CCWGG 4 cut(s) 413, 661, 730, 1272
PspN4I GGNNCC 4 cut(s) 159, 229, 276, 291
PspPI GGNCC 3 cut(s) 157, 228, 289
PspPPI RGGWCCY 1 cut(s) 228
PspXI VCTCGAGB 1 cut(s) 194
PsrI GAACNNNNNNTAC 2 cut(s) 56, 88
PstI CTGCAG 3 cut(s) 85, 1008, 1428
PstNI CAGNNNCTG 1 cut(s) 1432
PvuII CAGCTG 1 cut(s) 1009
RsaI GTAC 5 cut(s) 74, 358, 650, 692, 723
RsaNI GTAC 5 cut(s) 73, 357, 649, 691, 722
RseI CAYNNNNRTG 1 cut(s) 1215
SacI GAGCTC 2 cut(s) 108, 1098
SaqAI TTAA 3 cut(s) 467, 620, 990
SatI GCNGC 8 cut(s) 60, 81, 139, 206, 554, 776, 1007, 1404
Sau3AI GATC 6 cut(s) 738, 862, 1112, 1300, 1316, 1340
Sau96I GGNCC 3 cut(s) 157, 228, 289
SchI GAGTC 3 cut(s) 206, 1082, 1294
ScrFI CCNGG 7 cut(s) 161, 415, 663, 732, 1117, 1118, 1274
SduI GDGCHC 4 cut(s) 108, 243, 897, 1098
SfcI CTRYAG 4 cut(s) 81, 1004, 1424, 1489
Sfr274I CTCGAG 1 cut(s) 194
SinI GGWCC 3 cut(s) 157, 228, 289
SlaI CTCGAG 1 cut(s) 194
SmaI CCCGGG 1 cut(s) 1118
SmiMI CAYNNNNRTG 1 cut(s) 1215
SmlI CTYRAG 3 cut(s) 194, 466, 694
SmoI CTYRAG 3 cut(s) 194, 466, 694
SnaBI TACGTA 1 cut(s) 486
Sse9I AATT 9 cut(s) 30, 132, 171, 535, 743, 765, 1220, 1265, 1549
SseBI AGGCCT 2 cut(s) 477, 820
SsiI CCGC 6 cut(s) 38, 59, 123, 138, 254, 1347
SspMI CTAG 3 cut(s) 713, 1136, 1407
SstI GAGCTC 2 cut(s) 108, 1098
StuI AGGCCT 2 cut(s) 477, 820
StyD4I CCNGG 7 cut(s) 159, 413, 661, 730, 1115, 1116, 1272
StyI CCWWGG 1 cut(s) 1477
TaaI ACNGT 7 cut(s) 250, 392, 437, 880, 1183, 1308, 1490
TaiI ACGT 3 cut(s) 155, 488, 1132
TaqI TCGA 6 cut(s) 102, 195, 627, 905, 968, 1111
TasI AATT 9 cut(s) 30, 132, 171, 535, 743, 765, 1220, 1265, 1549
TatI WGTACW 3 cut(s) 72, 356, 721
TauI GCSGC 2 cut(s) 62, 141
TfiI GAWTC 7 cut(s) 144, 806, 907, 917, 1240, 1391, 1429
Tru1I TTAA 3 cut(s) 467, 620, 990
Tru9I TTAA 3 cut(s) 467, 620, 990
TscAI CASTG 5 cut(s) 82, 457, 529, 1311, 1428
TseFI GTSAC 1 cut(s) 52
TseI GCWGC 6 cut(s) 80, 205, 553, 775, 1006, 1403
Tsp45I GTSAC 1 cut(s) 52
TspDTI ATGAA 6 cut(s) 155, 366, 420, 819, 1167, 1233
TspGWI ACGGA 1 cut(s) 34
TspMI CCCGGG 1 cut(s) 1116
TspRI CASTG 5 cut(s) 82, 457, 529, 1311, 1428
Vha464I CTTAAG 1 cut(s) 466
VpaK11BI GGWCC 3 cut(s) 157, 228, 289
XapI RAATTY 2 cut(s) 765, 1265
XcmI CCANNNNNNNNNTGG 1 cut(s) 839
XhoI CTCGAG 1 cut(s) 194
XmaI CCCGGG 1 cut(s) 1116
XspI CTAG 3 cut(s) 713, 1136, 1407
Zsp2I ATGCAT 1 cut(s) 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.