pycom15g18450

Cytokinin dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0013976
Physical Location & Seq
Forward (+)
13123882 .. 13126736
2855 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1554 bp
ATGGCTGAGAGTTTTCCCTTCCCAACATTACTCATATTCTTGATCATTCTGAGCGGTCTGATTTTCACCATGGGTTATAAACTTCGTGACGATCCAGAAGCCATTAAATTAGCTTCTAGTGATTTTGGTCACATTCATAGATACAACCCAGCAGCCGTTTTGTACCCAGCTTCCATAAACGACATTTCAAGCTTGATCAAATTCTCAAACAAAGTTTCGACTCCTTTCAGCATAGCTGCTCGAGGCCATGGTCATTCCACCAGAGGACAGGCCATGGCCCCGAACGGTGTTGTGGTGGACATGACCTCACTGAAAAATTACCGTCCACATGTATCTGGAATTAGTGTTAATTCTTCTAAAAATTCGGGTATTAATATTAATAATAATGATAATTATGCTGATGTGGGAGGTGGACAGCTTTGGATTGATGTGCTGCATGCCACATTAGAACATGGACTTACACCGGTCTCGTGGACCGACTACCTTTACTTAACCGTTGGCGGGACTCTCTCCAACGCTGGCATCAGCGGCTCGGCCTTCCGGTTTGGTCCTCAGATTACCAATGTTTATGAAATGGATGTTGTCACTGGGCAAGGGGATTTTGTGACTTGCTCCCCACAAAAAAACTCCGAGCTCTTCTTTGGAGTCCTCGGAGGATTAGGTCAATTTGGGATTATAACTAGGGCAAGAATTTCCCTAGAGCCAGCACCTAAGAGGGTCAAGTGGGTACGAATGCTTTACAGTGACTTCTCTACCTTTGCCAGAGACCAAGAACGTTTAATTTCTCTCAACGGAAGAAAACAAAGCAACGCCCTCGATTATTTGGAAGGTTCACTTATGTTGAACCAGGGTCCTCCGGATAATTGGAGATCCTCCTTTTTTCCACAATCTTCTCACTCAAGAATTATTTCTCTTGTAACCAAACGGGGCATCATTTATTGTCTGGAAGTATCCAAATACTATGACGAGCACACTGAAACGACCGTGAAGAAGGAACTTGAACTTCTATTCAAAGGGTTGAGCTTTCTTCCTGGATTTTTGTTTGAGAAGGATGTGTCTTATGTCGATTTTCTGAATAGAGTTAGAAGTGGAGAGGAAAAGCTTCACTCACAAGGACAATGGGATGTCCCTCATCCATGGCTGAATCTTTTTATTCCAAAAAGTAGCATCTCGGACTTTGATGCAGGAGTGTTCAAGGACATTGTTCTCAGGAGAAACATTACCACAGGACCAGTCCTCGTTTATCCAATGAACCGGACCAAATGGGATGATAGGATGTCAGCGGTTATACCTCAAGAAGATGTTTTCTACACCGTAGGATTTTTGCATTCAAGTGGGTTTGACGATTGGGAAGCATTTGAGGAGCAAAACGAAGAGATATTAGGGTTTTGTGACAGGGCTGGTTTAGAGGTTAAGCAGTACCTTCCTCACATAAAAACACAGCAAGGTTGGATAAACCACTTTGGCTCTAAATGGAAAGCCTTCCTGAAGAAGAAAGCTTTGTTCGACCCCAAGAGGATACTCTCTCCGGGACAAAGAATTTTCAATAATTAA

Protein Analysis

518

Amino Acids

58.15

Weight (kDa)

7.74

Isoelectric Point (pI)

37.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 50 - 205 2.7e-17 FAD binding domain
Cytokin-bind PF09265 237 - 515 3e-119 Cytokinin dehydrogenase 1, FAD and cytokinin binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013976)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G56970 AT5G56970
fragaria_vesca FvH4_1g07620
malus_domestica MD15G1208300.v1.1
prunus_persica Prupe.7G208400_v2.0.a1 Prupe.7G208400_v2.0.a1
pyrus_communis pycom15g18450
rosa_chinensis RchiOBHm_Chr2g0093491
rosa_laevigata RLG00000016411
rosa_multiflora Rmu_sc0004423.1_g000008
rosa_roxburghii Rroxscaffold_2G00148040
rosa_rugosa Rorug02G0036700
rosa_samantha Rh2AG083200 Rh2BG083900 Rh2CG085900 Rh2DG082000
rosa_wichuraiana Rw2G006420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 78, 677
AccBSI CCGCTC 1 cut(s) 54
AccIII TCCGGA 1 cut(s) 856
AciI CCGC 4 cut(s) 54, 501, 528, 1283
AclI AACGTT 1 cut(s) 775
AclWI GGATC 2 cut(s) 86, 864
AcsI RAATTY 4 cut(s) 200, 361, 690, 1539
AcuI CTGAAG 1 cut(s) 1508
AfaI GTAC 3 cut(s) 164, 729, 1421
AfiI CCNNNNNNNGG 1 cut(s) 501
AflIII ACRYGT 1 cut(s) 328
AgeI ACCGGT 1 cut(s) 463
AgsI TTSAA 7 cut(s) 189, 844, 1001, 1012, 1195, 1332, 1546
AjnI CCWGG 2 cut(s) 846, 1030
AluBI AGCT 9 cut(s) 113, 170, 192, 236, 418, 634, 1023, 1102, 1499
AluI AGCT 9 cut(s) 113, 170, 192, 236, 418, 634, 1023, 1102, 1499
Alw21I GWGCWC 2 cut(s) 636, 972
Alw26I GTCTC 2 cut(s) 472, 759
AlwI GGATC 2 cut(s) 86, 864
Ama87I CYCGRG 1 cut(s) 240
Aor13HI TCCGGA 1 cut(s) 856
AoxI GGCC 4 cut(s) 244, 270, 276, 534
ApeKI GCWGC 3 cut(s) 152, 236, 433
ApoI RAATTY 4 cut(s) 200, 361, 690, 1539
AseI ATTAAT 2 cut(s) 372, 378
AsiGI ACCGGT 1 cut(s) 463
Asp700I GAANNNNTTC 3 cut(s) 907, 1101, 1481
AspS9I GGNCC 6 cut(s) 277, 474, 548, 851, 1229, 1257
AsuC2I CCSGG 1 cut(s) 1530
AsuHPI GGTGA 1 cut(s) 58
AvaI CYCGRG 1 cut(s) 240
AvaII GGWCC 5 cut(s) 474, 548, 851, 1229, 1257
BanII GRGCYC 1 cut(s) 636
BauI CACGAG 1 cut(s) 469
Bbv12I GWGCWC 2 cut(s) 636, 972
BbvI GCAGC 3 cut(s) 164, 223, 420
BceAI ACGGC 1 cut(s) 140
BcgI CGANNNNNNTGC 2 cut(s) 796, 830
BciT130I CCWGG 2 cut(s) 848, 1032
BciVI GTATCC 2 cut(s) 961, 1512
BclI TGATCA 2 cut(s) 42, 195
BcnI CCSGG 1 cut(s) 1530
BcoDI GTCTC 2 cut(s) 472, 759
BfaI CTAG 3 cut(s) 117, 681, 698
BfuI GTATCC 2 cut(s) 961, 1512
BisI GCNGC 4 cut(s) 153, 237, 434, 529
BlsI GCNGC 4 cut(s) 154, 238, 435, 530
Bme1390I CCNGG 3 cut(s) 848, 1032, 1530
Bme18I GGWCC 5 cut(s) 474, 548, 851, 1229, 1257
BmeT110I CYCGRG 1 cut(s) 240
BmgT120I GGNCC 6 cut(s) 277, 474, 548, 851, 1229, 1257
BmiI GGNNCC 2 cut(s) 279, 852
BmrFI CCNGG 3 cut(s) 848, 1032, 1530
BmrI ACTGGG 1 cut(s) 597
BmsI GCATC 4 cut(s) 531, 939, 1171, 1176
BmuI ACTGGG 1 cut(s) 597
BpuEI CTTGAG 2 cut(s) 883, 1278
BpuMI CCSGG 1 cut(s) 1530
BsaI GGTCTC 2 cut(s) 472, 759
BsaJI CCNNGG 6 cut(s) 69, 247, 273, 649, 847, 1136
BsaWI WCCGGW 4 cut(s) 463, 540, 856, 1254
Bsc4I CCNNNNNNNGG 1 cut(s) 501
Bse118I RCCGGY 1 cut(s) 463
Bse1I ACTGG 2 cut(s) 592, 1232
BseAI TCCGGA 1 cut(s) 856
BseBI CCWGG 2 cut(s) 848, 1032
BseDI CCNNGG 6 cut(s) 69, 247, 273, 649, 847, 1136
BseGI GGATG 6 cut(s) 583, 1057, 1129, 1132, 1273, 1281
BseLI CCNNNNNNNGG 1 cut(s) 501
BseMII CTCAG 3 cut(s) 41, 566, 1222
BseNI ACTGG 2 cut(s) 592, 1232
BseRI GAGGAG 1 cut(s) 1376
BseXI GCAGC 3 cut(s) 164, 223, 420
BseYI CCCAGC 2 cut(s) 148, 166
Bsh1285I CGRYCG 1 cut(s) 984
BshFI GGCC 4 cut(s) 246, 272, 278, 536
BshTI ACCGGT 1 cut(s) 463
BsiEI CGRYCG 1 cut(s) 984
BsiHKAI GWGCWC 2 cut(s) 636, 972
BsiHKCI CYCGRG 1 cut(s) 240
BsiSI CCGG 5 cut(s) 464, 541, 857, 1255, 1529
BslFI GGGAC 3 cut(s) 517, 1112, 1545
BslI CCNNNNNNNGG 1 cut(s) 501
BsmAI GTCTC 2 cut(s) 472, 759
BsmFI GGGAC 3 cut(s) 517, 1112, 1545
BsmI GAATGC 2 cut(s) 738, 1327
BsnI GGCC 4 cut(s) 246, 272, 278, 536
Bso31I GGTCTC 2 cut(s) 472, 759
BsoBI CYCGRG 1 cut(s) 240
Bsp1286I GDGCHC 2 cut(s) 636, 972
Bsp13I TCCGGA 1 cut(s) 856
Bsp143I GATC 4 cut(s) 42, 91, 195, 869
Bsp19I CCATGG 4 cut(s) 69, 247, 273, 1136
BspACI CCGC 4 cut(s) 54, 501, 528, 1283
BspANI GGCC 4 cut(s) 246, 272, 278, 536
BspCNI CTCAG 3 cut(s) 42, 565, 1221
BspEI TCCGGA 1 cut(s) 856
BspLI GGNNCC 2 cut(s) 279, 852
BspPI GGATC 2 cut(s) 86, 864
BspQI GCTCTTC 1 cut(s) 641
BspTNI GGTCTC 2 cut(s) 472, 759
BsrBI CCGCTC 1 cut(s) 54
BsrFI RCCGGY 1 cut(s) 463
BsrI ACTGG 2 cut(s) 592, 1232
BssAI RCCGGY 1 cut(s) 463
BssECI CCNNGG 6 cut(s) 69, 247, 273, 649, 847, 1136
BssMI GATC 4 cut(s) 42, 91, 195, 869
BssSI CACGAG 1 cut(s) 469
BssT1I CCWWGG 4 cut(s) 69, 247, 273, 1136
Bst2BI CACGAG 1 cut(s) 469
Bst2UI CCWGG 2 cut(s) 848, 1032
Bst4CI ACNGT 6 cut(s) 287, 323, 496, 743, 985, 1315
Bst6I CTCTTC 2 cut(s) 641, 1368
BstC8I GCNNGC 3 cut(s) 438, 520, 705
BstDEI CTNAG 5 cut(s) 6, 50, 552, 711, 1208
BstDSI CCRYGG 4 cut(s) 69, 247, 273, 1136
BstF5I GGATG 6 cut(s) 583, 1057, 1129, 1132, 1273, 1281
BstKTI GATC 4 cut(s) 45, 94, 198, 872
BstMAI GTCTC 2 cut(s) 472, 759
BstMBI GATC 4 cut(s) 42, 91, 195, 869
BstMCI CGRYCG 1 cut(s) 984
BstMWI GCNNNNNNNGC 1 cut(s) 528
BstNI CCWGG 2 cut(s) 848, 1032
BstNSI RCATGY 2 cut(s) 332, 440
BstSCI CCNGG 3 cut(s) 846, 1030, 1528
BstV1I GCAGC 3 cut(s) 164, 223, 420
BstX2I RGATCY 1 cut(s) 869
BstYI RGATCY 1 cut(s) 869
BsuI GTATCC 2 cut(s) 961, 1512
BsuRI GGCC 4 cut(s) 246, 272, 278, 536
BtgI CCRYGG 4 cut(s) 69, 247, 273, 1136
BtsCI GGATG 6 cut(s) 583, 1057, 1129, 1132, 1273, 1281
BtsIMutI CAGTG 4 cut(s) 308, 585, 748, 972
Cac8I GCNNGC 3 cut(s) 438, 520, 705
Cfr10I RCCGGY 1 cut(s) 463
Cfr13I GGNCC 6 cut(s) 277, 474, 548, 851, 1229, 1257
Csp6I GTAC 3 cut(s) 163, 728, 1420
CspAI ACCGGT 1 cut(s) 463
CviAII CATG 8 cut(s) 70, 248, 274, 301, 329, 437, 452, 1137
CviQI GTAC 3 cut(s) 163, 728, 1420
DdeI CTNAG 5 cut(s) 6, 50, 552, 711, 1208
DpnI GATC 4 cut(s) 44, 93, 197, 871
DpnII GATC 4 cut(s) 42, 91, 195, 869
Eam1104I CTCTTC 2 cut(s) 641, 1368
EarI CTCTTC 2 cut(s) 641, 1368
Ecl136II GAGCTC 1 cut(s) 634
Eco130I CCWWGG 4 cut(s) 69, 247, 273, 1136
Eco24I GRGCYC 1 cut(s) 636
Eco31I GGTCTC 2 cut(s) 472, 759
Eco47I GGWCC 5 cut(s) 474, 548, 851, 1229, 1257
Eco53kI GAGCTC 1 cut(s) 634
Eco57I CTGAAG 1 cut(s) 1508
Eco88I CYCGRG 1 cut(s) 240
EcoICRI GAGCTC 1 cut(s) 634
EcoO109I RGGNCCY 1 cut(s) 851
EcoRII CCWGG 2 cut(s) 846, 1030
EcoT14I CCWWGG 4 cut(s) 69, 247, 273, 1136
EcoT38I GRGCYC 1 cut(s) 636
ErhI CCWWGG 4 cut(s) 69, 247, 273, 1136
FaeI CATG 8 cut(s) 73, 251, 277, 304, 332, 440, 455, 1140
FalI AAGNNNNNCTT 2 cut(s) 819, 851
FaqI GGGAC 3 cut(s) 517, 1112, 1545
FatI CATG 8 cut(s) 69, 247, 273, 300, 328, 436, 451, 1136
FauI CCCGC 1 cut(s) 494
FbaI TGATCA 2 cut(s) 42, 195
Fnu4HI GCNGC 4 cut(s) 153, 237, 434, 529
FokI GGATG 6 cut(s) 590, 1064, 1119, 1136, 1280, 1288
FriOI GRGCYC 1 cut(s) 636
Fsp4HI GCNGC 4 cut(s) 153, 237, 434, 529
FspBI CTAG 3 cut(s) 117, 681, 698
GluI GCNGC 4 cut(s) 153, 237, 434, 529
GsaI CCCAGC 2 cut(s) 152, 170
HaeIII GGCC 4 cut(s) 246, 272, 278, 536
HapII CCGG 5 cut(s) 464, 541, 857, 1255, 1529
Hin1II CATG 8 cut(s) 73, 251, 277, 304, 332, 440, 455, 1140
HindIII AAGCTT 3 cut(s) 190, 1100, 1497
HinfI GANTC 4 cut(s) 220, 505, 645, 1144
HpaII CCGG 5 cut(s) 464, 541, 857, 1255, 1529
HphI GGTGA 1 cut(s) 58
Hpy166II GTNNAC 5 cut(s) 298, 326, 413, 474, 833
Hpy188I TCNGA 7 cut(s) 51, 60, 555, 631, 653, 1074, 1174
Hpy8I GTNNAC 5 cut(s) 298, 326, 413, 474, 833
HpyAV CCTTC 7 cut(s) 28, 547, 821, 985, 1042, 1433, 1492
HpyCH4III ACNGT 6 cut(s) 287, 323, 496, 743, 985, 1315
HpyCH4IV ACGT 1 cut(s) 775
HpyCH4V TGCA 3 cut(s) 436, 1184, 1327
HpyF10VI GCNNNNNNNGC 1 cut(s) 528
HpyF3I CTNAG 5 cut(s) 6, 50, 552, 711, 1208
HpySE526I ACGT 1 cut(s) 775
Hsp92II CATG 8 cut(s) 73, 251, 277, 304, 332, 440, 455, 1140
Kpn2I TCCGGA 1 cut(s) 856
Ksp22I TGATCA 2 cut(s) 42, 195
Kzo9I GATC 4 cut(s) 42, 91, 195, 869
LguI GCTCTTC 1 cut(s) 641
LmnI GCTCC 2 cut(s) 617, 1363
Lsp1109I GCAGC 3 cut(s) 164, 223, 420
LweI GCATC 4 cut(s) 531, 939, 1171, 1176
MaeI CTAG 3 cut(s) 117, 681, 698
MaeII ACGT 1 cut(s) 775
MaeIII GTNAC 7 cut(s) 86, 128, 583, 604, 743, 916, 1391
MalI GATC 4 cut(s) 44, 93, 197, 871
MbiI CCGCTC 1 cut(s) 54
MboI GATC 4 cut(s) 42, 91, 195, 869
MflI RGATCY 1 cut(s) 869
MhlI GDGCHC 2 cut(s) 636, 972
MlyI GAGTC 3 cut(s) 214, 499, 654
MmeI TCCRAC 2 cut(s) 537, 1430
MroI TCCGGA 1 cut(s) 856
MroXI GAANNNNTTC 3 cut(s) 907, 1101, 1481
MseI TTAA 8 cut(s) 105, 348, 372, 378, 491, 779, 1413, 1552
MslI CAYNNNNRTG 1 cut(s) 1332
MspA1I CMGCKG 2 cut(s) 528, 1283
MspI CCGG 5 cut(s) 464, 541, 857, 1255, 1529
MspR9I CCNGG 3 cut(s) 848, 1032, 1530
Mva1269I GAATGC 2 cut(s) 738, 1327
MvaI CCWGG 2 cut(s) 848, 1032
MwoI GCNNNNNNNGC 1 cut(s) 528
NciI CCSGG 1 cut(s) 1530
NcoI CCATGG 4 cut(s) 69, 247, 273, 1136
NdeII GATC 4 cut(s) 42, 91, 195, 869
NlaIII CATG 8 cut(s) 73, 251, 277, 304, 332, 440, 455, 1140
NlaIV GGNNCC 2 cut(s) 279, 852
NmeAIII GCCGAG 1 cut(s) 512
NmuCI GTSAC 6 cut(s) 86, 128, 583, 604, 743, 1391
NspI RCATGY 2 cut(s) 332, 440
PaeI GCATGC 1 cut(s) 440
PaeR7I CTCGAG 1 cut(s) 240
PciI ACATGT 1 cut(s) 328
PciSI GCTCTTC 1 cut(s) 641
PctI GAATGC 2 cut(s) 738, 1327
PdmI GAANNNNTTC 3 cut(s) 907, 1101, 1481
PfeI GAWTC 1 cut(s) 1144
PfoI TCCNGGA 2 cut(s) 1030, 1528
PinAI ACCGGT 1 cut(s) 463
PkrI GCNGC 4 cut(s) 154, 238, 435, 530
PleI GAGTC 3 cut(s) 214, 499, 653
PpsI GAGTC 3 cut(s) 214, 499, 653
PpuMI RGGWCCY 1 cut(s) 851
PscI ACATGT 1 cut(s) 328
PshBI ATTAAT 2 cut(s) 372, 378
PsiI TTATAA 2 cut(s) 78, 677
Psp124BI GAGCTC 1 cut(s) 636
Psp1406I AACGTT 1 cut(s) 775
Psp5II RGGWCCY 1 cut(s) 851
Psp6I CCWGG 2 cut(s) 846, 1030
PspFI CCCAGC 2 cut(s) 148, 166
PspGI CCWGG 2 cut(s) 846, 1030
PspN4I GGNNCC 2 cut(s) 279, 852
PspPI GGNCC 6 cut(s) 277, 474, 548, 851, 1229, 1257
PspPPI RGGWCCY 1 cut(s) 851
PspXI VCTCGAGB 1 cut(s) 240
PsuI RGATCY 1 cut(s) 869
RsaI GTAC 3 cut(s) 164, 729, 1421
RsaNI GTAC 3 cut(s) 163, 728, 1420
RseI CAYNNNNRTG 1 cut(s) 1332
SacI GAGCTC 1 cut(s) 636
SapI GCTCTTC 1 cut(s) 641
SaqAI TTAA 8 cut(s) 105, 348, 372, 378, 491, 779, 1413, 1552
SatI GCNGC 4 cut(s) 153, 237, 434, 529
Sau3AI GATC 4 cut(s) 42, 91, 195, 869
Sau96I GGNCC 6 cut(s) 277, 474, 548, 851, 1229, 1257
SchI GAGTC 3 cut(s) 214, 499, 654
ScrFI CCNGG 3 cut(s) 848, 1032, 1530
SduI GDGCHC 2 cut(s) 636, 972
SfaNI GCATC 4 cut(s) 531, 939, 1171, 1176
Sfr274I CTCGAG 1 cut(s) 240
SinI GGWCC 5 cut(s) 474, 548, 851, 1229, 1257
SlaI CTCGAG 1 cut(s) 240
SmiMI CAYNNNNRTG 1 cut(s) 1332
SmlI CTYRAG 3 cut(s) 240, 898, 1293
SmoI CTYRAG 3 cut(s) 240, 898, 1293
SphI GCATGC 1 cut(s) 440
SsiI CCGC 4 cut(s) 54, 501, 528, 1283
SspI AATATT 1 cut(s) 376
SspMI CTAG 3 cut(s) 117, 681, 698
SstI GAGCTC 1 cut(s) 636
StyD4I CCNGG 3 cut(s) 846, 1030, 1528
StyI CCWWGG 4 cut(s) 69, 247, 273, 1136
TaaI ACNGT 6 cut(s) 287, 323, 496, 743, 985, 1315
TaiI ACGT 1 cut(s) 778
TaqI TCGA 5 cut(s) 218, 241, 816, 1065, 1506
TaqII GACCGA 1 cut(s) 491
TauI GCSGC 1 cut(s) 531
TfiI GAWTC 1 cut(s) 1144
Tru1I TTAA 8 cut(s) 105, 348, 372, 378, 491, 779, 1413, 1552
Tru9I TTAA 8 cut(s) 105, 348, 372, 378, 491, 779, 1413, 1552
TscAI CASTG 4 cut(s) 315, 592, 748, 979
TseFI GTSAC 6 cut(s) 86, 128, 583, 604, 743, 1391
TseI GCWGC 3 cut(s) 152, 236, 433
Tsp45I GTSAC 6 cut(s) 86, 128, 583, 604, 743, 1391
TspDTI ATGAA 3 cut(s) 125, 585, 1265
TspGWI ACGGA 1 cut(s) 807
TspRI CASTG 4 cut(s) 315, 592, 748, 979
VpaK11BI GGWCC 5 cut(s) 474, 548, 851, 1229, 1257
VspI ATTAAT 2 cut(s) 372, 378
XapI RAATTY 4 cut(s) 200, 361, 690, 1539
XceI RCATGY 2 cut(s) 332, 440
XhoI CTCGAG 1 cut(s) 240
XmnI GAANNNNTTC 3 cut(s) 907, 1101, 1481
XspI CTAG 3 cut(s) 117, 681, 698
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.