pycom15g20160

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0016727
Physical Location & Seq
Reverse (-)
14391463 .. 14393522
2060 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1590 bp
ATGACTTATAAGGGATTGGCAGCTGGAGGGGCAAGAGGAGGTGCACCAATGGCTAGGATTGCAGTGTACAAGACATGCTGGAACTCTGGTTGTTATGACGTTGACCTACTAGCGGCCTTTGATGATGCAATCAGAGACGGGGTTGATATCATGTCTCTGTCTCTTGGTCCTGATGCTCCTCAGGGAGACTATTTCAGTGATGCCATTTCTGTCGGATCATTTCATGCTGTTAGGCATGGAATTTTAGTGGTTGCTTCTTCTGGAAATGAAGGAAACCCGGGTTCTGCAACAAATTTAGCCCCATGGATGCTCACTGTTGCAGCAAGTTCAACAGATAGGGATTTCACTTCTGATATCATACTAGAAAATGGTGCTAATTTCACGGGTGAAAGTCTCAGTCTCTTTGAAATGAAAGCCTCAGCAAGAATTATTTCCGCCTCTGAAGCCAATGCAGGGTATTTTACTCCGTATCAATCCAGTTACTGTTTAGAAAGTTCCTTGAATAGAACCAAGGCCAGAGGGAAGGTTCTGGTCTGTCGACATGCTGAGAGTTCAACAGAGTCAAAGCTCACTAAAAGTATGCTAGTGAAAAAAGCTGGTGGTGTCGGGATGGTTCTCATTGATGACGCAGATAAGGATATTGCTGTCCCATTTGTCATCCCTTCAGCTATTGTTGAACAGAGGATAGGGCATCATATTTTATCTTATATTAAACGCACACGGAAACCCATGTCTCGAATTTTGCCCGCAAAGACTATACTGGGACTGAAACCTGCACCTCGTGTTACTGCGTTTTCTTCGAAAGGCCCCAATTCTTTGACACCGGAGATTTTGAAGCCCGATGTCACAGCTCCTGGACTGAATATACTAGCATCTTGGTCCCCAGCAGCAGGGAATAAGCAGTTCAACATTCTTTCTGGTACTTCCATGGCTTGCCCACATGTAACAGGAATCGCTGCCTTGATCAAAGCTGTTTATCCATCATGGTCCCCAGCAGCTATTAGATCTGCAATCATGACTACAGCTACTCTTCTGGATAAGAACCACAAACCCATCCTAGTGGACCCTGAAGGAAGAAGGGGTAACGCATTCGATTATGGCTCAGGCTTTGTGAACCCAAAAAGAGTCCTTGACCCCGGTCTTGTATACGACGTGCATTCAGCAGACTACGTAGAATTCCTTTGTTCAGTTGGTTATGACGAGAAAGCAGTCCATCAAATCACACAAGACAACAGTACATGTAAGCAGGCATTTCGAACAGCATCTGACCTGAATTATCCATCTATCACAGTGCCAAATCTTGTAGGTAACTTCTCGGTAACTAGAACAGTGACCAATGTTGGTAAGCCAAAGAGTATATATAAAGCTGTGGTGTCCTCACCCGTTGGAATCAATGTTACTATTGTCCCGAATCGATTGATCTTCAATAGGTTTGGCCAGAAGATACAGTTCACTGTAAACTTCAAGGTGGCTGCTCCACCATCGAAGGGATATGCATTCGGGTTCTTTTCATGGGTAAGTGGACGATCAAGAGTTACCAGCCCCCTGGTTGTCCGAGTTGCACATTCGAACTCAGGGCTGCTGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

530

Amino Acids

56.53

Weight (kDa)

9.44

Isoelectric Point (pI)

35.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 8 - 350 2.7e-39 Subtilase family
fn3_6 PF17766 423 - 518 7.9e-29 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016727)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g09800
malus_domestica MD02G1107500.v1.1 MD15G1226700.v1.1
prunus_persica Prupe.7G188500_v2.0.a1
pyrus_communis pycom02g08610 pycom15g20160
rosa_chinensis RchiOBHm_Chr2g0096651
rosa_laevigata RLG00000016658
rosa_multiflora Rmu_sc0003565.1_g000017
rosa_roxburghii Rroxscaffold_2G00145450
rosa_rugosa Rorug02G0059200
rosa_samantha Rh2BG109100 Rh2DG110400
rosa_wichuraiana Rw2G008260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 9
Acc36I ACCTGC 1 cut(s) 781
AccI GTMKAC 2 cut(s) 538, 1146
AciI CCGC 3 cut(s) 113, 435, 747
AclWI GGATC 1 cut(s) 223
AcoI YGGCCR 1 cut(s) 1435
AcsI RAATTY 4 cut(s) 240, 292, 738, 1175
AcuI CTGAAG 3 cut(s) 462, 648, 1089
AdeI CACNNNGTG 1 cut(s) 782
AfaI GTAC 3 cut(s) 68, 922, 1237
AfiI CCNNNNNNNGG 4 cut(s) 112, 453, 890, 1487
AflIII ACRYGT 2 cut(s) 940, 1238
AgsI TTSAA 9 cut(s) 330, 407, 502, 555, 677, 835, 907, 1426, 1465
AjiI CACGTC 1 cut(s) 1153
AjnI CCWGG 2 cut(s) 853, 1545
AluBI AGCT 9 cut(s) 23, 568, 596, 668, 851, 971, 998, 1025, 1367
AluI AGCT 9 cut(s) 23, 568, 596, 668, 851, 971, 998, 1025, 1367
Alw21I GWGCWC 1 cut(s) 46
Alw26I GTCTC 7 cut(s) 129, 159, 165, 180, 398, 404, 738
Alw44I GTGCAC 1 cut(s) 42
AlwI GGATC 1 cut(s) 223
AlwNI CAGNNNCTG 3 cut(s) 483, 854, 1265
Ama87I CYCGRG 1 cut(s) 277
AoxI GGCC 4 cut(s) 114, 513, 805, 1435
ApaLI GTGCAC 1 cut(s) 42
ApeKI GCWGC 7 cut(s) 20, 320, 887, 956, 995, 1472, 1579
ApoI RAATTY 4 cut(s) 240, 292, 738, 1175
Asp700I GAANNNNTTC 1 cut(s) 430
AspS9I GGNCC 5 cut(s) 167, 806, 879, 987, 1063
AsuC2I CCSGG 3 cut(s) 278, 279, 1137
AsuHPI GGTGA 2 cut(s) 398, 1371
AsuII TTCGAA 3 cut(s) 800, 1255, 1568
AvaI CYCGRG 1 cut(s) 277
AvaII GGWCC 4 cut(s) 167, 879, 987, 1063
AxyI CCTNAGG 1 cut(s) 180
BaeGI GKGCMC 1 cut(s) 46
BalI TGGCCA 1 cut(s) 1437
BauI CACGAG 1 cut(s) 780
Bbv12I GWGCWC 1 cut(s) 46
BbvCI CCTCAGC 1 cut(s) 418
BbvI GCAGC 7 cut(s) 32, 332, 899, 943, 1007, 1459, 1566
BccI CCATC 6 cut(s) 604, 988, 1061, 1221, 1288, 1489
BcgI CGANNNNNNTGC 2 cut(s) 1235, 1269
BciT130I CCWGG 2 cut(s) 855, 1547
BclI TGATCA 1 cut(s) 963
BcnI CCSGG 3 cut(s) 278, 279, 1137
BcoDI GTCTC 7 cut(s) 129, 159, 165, 180, 398, 404, 738
BfaI CTAG 7 cut(s) 54, 110, 362, 584, 869, 1058, 1323
BfmI CTRYAG 1 cut(s) 1020
BfuAI ACCTGC 1 cut(s) 781
BglII AGATCT 1 cut(s) 1004
BisI GCNGC 8 cut(s) 21, 114, 321, 888, 957, 996, 1473, 1580
BlsI GCNGC 8 cut(s) 22, 115, 322, 889, 958, 997, 1474, 1581
Bme1390I CCNGG 5 cut(s) 278, 279, 855, 1137, 1547
Bme18I GGWCC 4 cut(s) 167, 879, 987, 1063
BmeT110I CYCGRG 1 cut(s) 277
BmgBI CACGTC 1 cut(s) 1153
BmgT120I GGNCC 5 cut(s) 167, 806, 879, 987, 1063
BmiI GGNNCC 4 cut(s) 808, 881, 989, 1065
BmrFI CCNGG 5 cut(s) 278, 279, 855, 1137, 1547
BmrI ACTGGG 1 cut(s) 770
BmsI GCATC 7 cut(s) 115, 163, 190, 297, 700, 881, 1271
BmuI ACTGGG 1 cut(s) 770
BoxI GACNNNNGTC 1 cut(s) 1137
BpmI CTGGAG 1 cut(s) 45
Bpu10I CCTNAGC 2 cut(s) 418, 1102
Bpu14I TTCGAA 3 cut(s) 800, 1255, 1568
BpuMI CCSGG 3 cut(s) 278, 279, 1137
Bsa29I ATCGAT 1 cut(s) 1414
BsaAI YACGTR 1 cut(s) 1171
BsaJI CCNNGG 6 cut(s) 277, 302, 510, 927, 1135, 1545
BsaWI WCCGGW 1 cut(s) 823
Bsc4I CCNNNNNNNGG 4 cut(s) 112, 453, 890, 1487
Bse1I ACTGG 2 cut(s) 477, 765
Bse21I CCTNAGG 1 cut(s) 180
BseBI CCWGG 2 cut(s) 855, 1547
BseCI ATCGAT 1 cut(s) 1414
BseDI CCNNGG 6 cut(s) 277, 302, 510, 927, 1135, 1545
BseGI GGATG 4 cut(s) 312, 615, 657, 1053
BseLI CCNNNNNNNGG 4 cut(s) 112, 453, 890, 1487
BseMII CTCAG 7 cut(s) 194, 409, 432, 537, 1116, 1574, 1587
BseNI ACTGG 2 cut(s) 477, 765
BseRI GAGGAG 2 cut(s) 51, 168
BseSI GKGCMC 1 cut(s) 46
BseXI GCAGC 7 cut(s) 32, 332, 899, 943, 1007, 1459, 1566
BseYI CCCAGC 2 cut(s) 883, 991
BsgI GTGCAG 1 cut(s) 759
BshFI GGCC 4 cut(s) 116, 515, 807, 1437
BshVI ATCGAT 1 cut(s) 1414
BsiHKAI GWGCWC 1 cut(s) 46
BsiHKCI CYCGRG 1 cut(s) 277
BsiSI CCGG 3 cut(s) 278, 824, 1137
BslFI GGGAC 5 cut(s) 632, 777, 865, 973, 1391
BslI CCNNNNNNNGG 4 cut(s) 112, 453, 890, 1487
BsmAI GTCTC 7 cut(s) 129, 159, 165, 180, 398, 404, 738
BsmBI CGTCTC 1 cut(s) 129
BsmFI GGGAC 5 cut(s) 632, 777, 865, 973, 1391
BsmI GAATGC 3 cut(s) 1088, 1156, 1496
BsnI GGCC 4 cut(s) 116, 515, 807, 1437
BsoBI CYCGRG 1 cut(s) 277
Bsp119I TTCGAA 3 cut(s) 800, 1255, 1568
Bsp1286I GDGCHC 1 cut(s) 46
Bsp1407I TGTACA 1 cut(s) 66
Bsp143I GATC 5 cut(s) 215, 963, 1004, 1419, 1526
Bsp19I CCATGG 2 cut(s) 302, 927
BspACI CCGC 3 cut(s) 113, 435, 747
BspANI GGCC 4 cut(s) 116, 515, 807, 1437
BspCNI CTCAG 7 cut(s) 193, 408, 431, 538, 1115, 1575, 1586
BspDI ATCGAT 1 cut(s) 1414
BspHI TCATGA 1 cut(s) 1014
BspLI GGNNCC 4 cut(s) 808, 881, 989, 1065
BspMI ACCTGC 1 cut(s) 781
BspPI GGATC 1 cut(s) 223
BspT104I TTCGAA 3 cut(s) 800, 1255, 1568
BsrGI TGTACA 1 cut(s) 66
BsrI ACTGG 2 cut(s) 477, 765
BssECI CCNNGG 6 cut(s) 277, 302, 510, 927, 1135, 1545
BssMI GATC 5 cut(s) 215, 963, 1004, 1419, 1526
BssNAI GTATAC 1 cut(s) 1147
BssSI CACGAG 1 cut(s) 780
BssT1I CCWWGG 3 cut(s) 302, 510, 927
Bst1107I GTATAC 1 cut(s) 1147
Bst2BI CACGAG 1 cut(s) 780
Bst2UI CCWGG 2 cut(s) 855, 1547
Bst4CI ACNGT 7 cut(s) 316, 485, 1235, 1291, 1330, 1449, 1456
Bst6I CTCTTC 1 cut(s) 1035
BstAUI TGTACA 1 cut(s) 66
BstBAI YACGTR 1 cut(s) 1171
BstBI TTCGAA 3 cut(s) 800, 1255, 1568
BstC8I GCNNGC 3 cut(s) 747, 934, 1248
BstDEI CTNAG 7 cut(s) 180, 395, 418, 546, 1102, 1573, 1583
BstDSI CCRYGG 2 cut(s) 302, 927
BstF5I GGATG 4 cut(s) 312, 615, 657, 1053
BstKTI GATC 5 cut(s) 218, 966, 1007, 1422, 1529
BstMAI GTCTC 7 cut(s) 129, 159, 165, 180, 398, 404, 738
BstMBI GATC 5 cut(s) 215, 963, 1004, 1419, 1526
BstMWI GCNNNNNNNGC 4 cut(s) 29, 50, 59, 443
BstNI CCWGG 2 cut(s) 855, 1547
BstNSI RCATGY 4 cut(s) 78, 545, 944, 1242
BstPAI GACNNNNGTC 1 cut(s) 1137
BstSCI CCNGG 5 cut(s) 276, 277, 853, 1135, 1545
BstSFI CTRYAG 1 cut(s) 1020
BstSLI GKGCMC 1 cut(s) 46
BstSNI TACGTA 1 cut(s) 1171
BstV1I GCAGC 7 cut(s) 32, 332, 899, 943, 1007, 1459, 1566
BstX2I RGATCY 1 cut(s) 1004
BstXI CCANNNNNNTGG 2 cut(s) 1060, 1546
BstYI RGATCY 1 cut(s) 1004
BstZ17I GTATAC 1 cut(s) 1147
Bsu15I ATCGAT 1 cut(s) 1414
Bsu36I CCTNAGG 1 cut(s) 180
BsuRI GGCC 4 cut(s) 116, 515, 807, 1437
BsuTUI ATCGAT 1 cut(s) 1414
BtgI CCRYGG 2 cut(s) 302, 927
BtrI CACGTC 1 cut(s) 1153
BtsCI GGATG 4 cut(s) 312, 615, 657, 1053
BtsI GCAGTG 1 cut(s) 69
BtsIMutI CAGTG 6 cut(s) 69, 202, 312, 1296, 1335, 1452
BveI ACCTGC 1 cut(s) 781
Cac8I GCNNGC 3 cut(s) 747, 934, 1248
CaiI CAGNNNCTG 3 cut(s) 483, 854, 1265
CciI TCATGA 1 cut(s) 1014
Cfr13I GGNCC 5 cut(s) 167, 806, 879, 987, 1063
Cfr9I CCCGGG 1 cut(s) 277
ClaI ATCGAT 1 cut(s) 1414
CseI GACGC 1 cut(s) 635
Csp6I GTAC 3 cut(s) 67, 921, 1236
CviQI GTAC 3 cut(s) 67, 921, 1236
DdeI CTNAG 7 cut(s) 180, 395, 418, 546, 1102, 1573, 1583
DpnI GATC 5 cut(s) 217, 965, 1006, 1421, 1528
DpnII GATC 5 cut(s) 215, 963, 1004, 1419, 1526
DraIII CACNNNGTG 1 cut(s) 782
EaeI YGGCCR 1 cut(s) 1435
Eam1104I CTCTTC 1 cut(s) 1035
EarI CTCTTC 1 cut(s) 1035
EciI GGCGGA 1 cut(s) 424
Eco105I TACGTA 1 cut(s) 1171
Eco130I CCWWGG 3 cut(s) 302, 510, 927
Eco32I GATATC 2 cut(s) 148, 355
Eco47I GGWCC 4 cut(s) 167, 879, 987, 1063
Eco57I CTGAAG 3 cut(s) 462, 648, 1089
Eco81I CCTNAGG 1 cut(s) 180
Eco88I CYCGRG 1 cut(s) 277
EcoO109I RGGNCCY 1 cut(s) 806
EcoRI GAATTC 1 cut(s) 1175
EcoRII CCWGG 2 cut(s) 853, 1545
EcoRV GATATC 2 cut(s) 148, 355
EcoT14I CCWWGG 3 cut(s) 302, 510, 927
EcoT22I ATGCAT 1 cut(s) 1498
ErhI CCWWGG 3 cut(s) 302, 510, 927
Esp3I CGTCTC 1 cut(s) 129
FaqI GGGAC 5 cut(s) 632, 777, 865, 973, 1391
FauI CCCGC 1 cut(s) 754
FbaI TGATCA 1 cut(s) 963
FblI GTMKAC 2 cut(s) 538, 1146
Fnu4HI GCNGC 8 cut(s) 21, 114, 321, 888, 957, 996, 1473, 1580
FokI GGATG 4 cut(s) 319, 622, 644, 1040
Fsp4HI GCNGC 8 cut(s) 21, 114, 321, 888, 957, 996, 1473, 1580
FspBI CTAG 7 cut(s) 54, 110, 362, 584, 869, 1058, 1323
GluI GCNGC 8 cut(s) 21, 114, 321, 888, 957, 996, 1473, 1580
GsaI CCCAGC 2 cut(s) 887, 995
GsuI CTGGAG 1 cut(s) 45
HaeIII GGCC 4 cut(s) 116, 515, 807, 1437
HapII CCGG 3 cut(s) 278, 824, 1137
HgaI GACGC 1 cut(s) 635
HincII GTYRAC 2 cut(s) 103, 539
HindII GTYRAC 2 cut(s) 103, 539
HinfI GANTC 5 cut(s) 560, 951, 1125, 1389, 1411
HpaII CCGG 3 cut(s) 278, 824, 1137
HphI GGTGA 2 cut(s) 398, 1371
Hpy188I TCNGA 6 cut(s) 134, 215, 352, 442, 1267, 1556
Hpy188III TCNNGA 8 cut(s) 170, 261, 607, 735, 1015, 1034, 1408, 1530
Hpy99I CGWCG 1 cut(s) 1154
HpyAV CCTTC 6 cut(s) 263, 517, 672, 1064, 1071, 1480
HpyCH4III ACNGT 7 cut(s) 316, 485, 1235, 1291, 1330, 1449, 1456
HpyCH4IV ACGT 3 cut(s) 99, 1152, 1170
HpyF10VI GCNNNNNNNGC 4 cut(s) 29, 50, 59, 443
HpyF3I CTNAG 7 cut(s) 180, 395, 418, 546, 1102, 1573, 1583
HpySE526I ACGT 3 cut(s) 99, 1152, 1170
Ksp22I TGATCA 1 cut(s) 963
Kzo9I GATC 5 cut(s) 215, 963, 1004, 1419, 1526
LmnI GCTCC 3 cut(s) 181, 856, 1480
Lsp1109I GCAGC 7 cut(s) 32, 332, 899, 943, 1007, 1459, 1566
LweI GCATC 7 cut(s) 115, 163, 190, 297, 700, 881, 1271
MaeI CTAG 7 cut(s) 54, 110, 362, 584, 869, 1058, 1323
MaeII ACGT 3 cut(s) 99, 1152, 1170
MalI GATC 5 cut(s) 217, 965, 1006, 1421, 1528
MboI GATC 5 cut(s) 215, 963, 1004, 1419, 1526
MboII GAAGA 6 cut(s) 249, 789, 1022, 1086, 1414, 1453
MflI RGATCY 1 cut(s) 1004
MhlI GDGCHC 1 cut(s) 46
MlsI TGGCCA 1 cut(s) 1437
MluCI AATT 8 cut(s) 240, 292, 376, 426, 738, 811, 1175, 1273
MluNI TGGCCA 1 cut(s) 1437
MlyI GAGTC 2 cut(s) 569, 1134
MmeI TCCRAC 2 cut(s) 193, 1366
Mox20I TGGCCA 1 cut(s) 1437
Mph1103I ATGCAT 1 cut(s) 1498
MroXI GAANNNNTTC 1 cut(s) 430
MscI TGGCCA 1 cut(s) 1437
MseI TTAA 1 cut(s) 711
MslI CAYNNNNRTG 1 cut(s) 1058
Msp20I TGGCCA 1 cut(s) 1437
MspA1I CMGCKG 1 cut(s) 23
MspI CCGG 3 cut(s) 278, 824, 1137
MspR9I CCNGG 5 cut(s) 278, 279, 855, 1137, 1547
Mva1269I GAATGC 3 cut(s) 1088, 1156, 1496
MvaI CCWGG 2 cut(s) 855, 1547
MwoI GCNNNNNNNGC 4 cut(s) 29, 50, 59, 443
NciI CCSGG 3 cut(s) 278, 279, 1137
NcoI CCATGG 2 cut(s) 302, 927
NdeII GATC 5 cut(s) 215, 963, 1004, 1419, 1526
NlaIV GGNNCC 4 cut(s) 808, 881, 989, 1065
NmuCI GTSAC 2 cut(s) 844, 1330
NsiI ATGCAT 1 cut(s) 1498
NspI RCATGY 4 cut(s) 78, 545, 944, 1242
NspV TTCGAA 3 cut(s) 800, 1255, 1568
PagI TCATGA 1 cut(s) 1014
PciI ACATGT 2 cut(s) 940, 1238
PctI GAATGC 3 cut(s) 1088, 1156, 1496
PdmI GAANNNNTTC 1 cut(s) 430
PfeI GAWTC 3 cut(s) 951, 1389, 1411
PfoI TCCNGGA 1 cut(s) 853
PkrI GCNGC 8 cut(s) 22, 115, 322, 889, 958, 997, 1474, 1581
PleI GAGTC 2 cut(s) 568, 1133
PpsI GAGTC 2 cut(s) 568, 1133
Ppu21I YACGTR 1 cut(s) 1171
PscI ACATGT 2 cut(s) 940, 1238
PshAI GACNNNNGTC 1 cut(s) 1137
PsiI TTATAA 1 cut(s) 9
Psp6I CCWGG 2 cut(s) 853, 1545
PspFI CCCAGC 2 cut(s) 883, 991
PspGI CCWGG 2 cut(s) 853, 1545
PspN4I GGNNCC 4 cut(s) 808, 881, 989, 1065
PspPI GGNCC 5 cut(s) 167, 806, 879, 987, 1063
PstNI CAGNNNCTG 3 cut(s) 483, 854, 1265
PsuI RGATCY 1 cut(s) 1004
PvuII CAGCTG 1 cut(s) 23
RsaI GTAC 3 cut(s) 68, 922, 1237
RsaNI GTAC 3 cut(s) 67, 921, 1236
RseI CAYNNNNRTG 1 cut(s) 1058
SalI GTCGAC 1 cut(s) 537
SaqAI TTAA 1 cut(s) 711
SatI GCNGC 8 cut(s) 21, 114, 321, 888, 957, 996, 1473, 1580
Sau3AI GATC 5 cut(s) 215, 963, 1004, 1419, 1526
Sau96I GGNCC 5 cut(s) 167, 806, 879, 987, 1063
SchI GAGTC 2 cut(s) 569, 1134
ScrFI CCNGG 5 cut(s) 278, 279, 855, 1137, 1547
SduI GDGCHC 1 cut(s) 46
SfaNI GCATC 7 cut(s) 115, 163, 190, 297, 700, 881, 1271
SfcI CTRYAG 1 cut(s) 1020
SfuI TTCGAA 3 cut(s) 800, 1255, 1568
SinI GGWCC 4 cut(s) 167, 879, 987, 1063
SmaI CCCGGG 1 cut(s) 279
SmiMI CAYNNNNRTG 1 cut(s) 1058
SnaBI TACGTA 1 cut(s) 1171
Sse9I AATT 8 cut(s) 240, 292, 376, 426, 738, 811, 1175, 1273
SsiI CCGC 3 cut(s) 113, 435, 747
SspMI CTAG 7 cut(s) 54, 110, 362, 584, 869, 1058, 1323
StyD4I CCNGG 5 cut(s) 276, 277, 853, 1135, 1545
StyI CCWWGG 3 cut(s) 302, 510, 927
TaaI ACNGT 7 cut(s) 316, 485, 1235, 1291, 1330, 1449, 1456
TaiI ACGT 3 cut(s) 102, 1155, 1173
TaqI TCGA 8 cut(s) 538, 736, 800, 1092, 1255, 1414, 1484, 1568
TasI AATT 8 cut(s) 240, 292, 376, 426, 738, 811, 1175, 1273
TatI WGTACW 2 cut(s) 66, 1235
TauI GCSGC 1 cut(s) 116
TfiI GAWTC 3 cut(s) 951, 1389, 1411
Tru1I TTAA 1 cut(s) 711
Tru9I TTAA 1 cut(s) 711
TscAI CASTG 6 cut(s) 69, 202, 319, 1296, 1335, 1459
TseFI GTSAC 2 cut(s) 844, 1330
TseI GCWGC 7 cut(s) 20, 320, 887, 956, 995, 1472, 1579
Tsp45I GTSAC 2 cut(s) 844, 1330
TspDTI ATGAA 4 cut(s) 212, 282, 425, 1500
TspGWI ACGGA 2 cut(s) 456, 736
TspMI CCCGGG 1 cut(s) 277
TspRI CASTG 6 cut(s) 69, 202, 319, 1296, 1335, 1459
VneI GTGCAC 1 cut(s) 42
VpaK11BI GGWCC 4 cut(s) 167, 879, 987, 1063
XapI RAATTY 4 cut(s) 240, 292, 738, 1175
XceI RCATGY 4 cut(s) 78, 545, 944, 1242
XmaI CCCGGG 1 cut(s) 277
XmiI GTMKAC 2 cut(s) 538, 1146
XmnI GAANNNNTTC 1 cut(s) 430
XspI CTAG 7 cut(s) 54, 110, 362, 584, 869, 1058, 1323
Zsp2I ATGCAT 1 cut(s) 1498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.