Methyltransferase
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT3G51070 AT3G51070 |
| fragaria_vesca | FvH4_1g12411 |
| malus_domestica | MD02G1136800.v1.1 MD15G1250600.v1.1 |
| prunus_persica | Prupe.7G163700_v2.0.a1 |
| pyrus_communis | pycom15g22090 |
| rosa_chinensis | RchiOBHm_Chr2g0100281 |
| rosa_laevigata | RLG00000016952 |
| rosa_multiflora | Rmu_sc0002547.1_g000021 Rmu_sc0002547.1_g000022 |
| rosa_roxburghii | Rroxscaffold_2G00142420 |
| rosa_rugosa | Rorug02G0087100 Rorug02G0087200 |
| rosa_samantha | Rh2AG136100 Rh2BG139700 Rh2CG141400 Rh2DG141600 |
| rosa_wichuraiana | Rw2G010650 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 2832 |
| Acc36I | ACCTGC | 1 cut(s) | 1819 |
| AccB1I | GGYRCC | 2 cut(s) | 608, 1610 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 1470, 2055 |
| AccBSI | CCGCTC | 1 cut(s) | 510 |
| AccI | GTMKAC | 3 cut(s) | 1214, 1376, 2109 |
| AccII | CGCG | 3 cut(s) | 22, 905, 1469 |
| AccIII | TCCGGA | 1 cut(s) | 1324 |
| AclWI | GGATC | 1 cut(s) | 510 |
| AcoI | YGGCCR | 5 cut(s) | 102, 1412, 1472, 2100, 2849 |
| AcsI | RAATTY | 6 cut(s) | 49, 812, 838, 2013, 2089, 2577 |
| AcuI | CTGAAG | 1 cut(s) | 2510 |
| AcvI | CACGTG | 2 cut(s) | 1296, 1428 |
| AcyI | GRCGYC | 1 cut(s) | 1611 |
| AflIII | ACRYGT | 1 cut(s) | 1293 |
| AgeI | ACCGGT | 1 cut(s) | 2229 |
| AgsI | TTSAA | 5 cut(s) | 348, 1637, 1936, 2364, 2770 |
| AhlI | ACTAGT | 1 cut(s) | 371 |
| AjnI | CCWGG | 3 cut(s) | 1431, 2198, 2707 |
| AluBI | AGCT | 8 cut(s) | 337, 542, 1303, 1710, 1778, 2319, 2462, 2486 |
| AluI | AGCT | 8 cut(s) | 337, 542, 1303, 1710, 1778, 2319, 2462, 2486 |
| Alw21I | GWGCWC | 4 cut(s) | 160, 1395, 2379, 2744 |
| Alw26I | GTCTC | 3 cut(s) | 932, 1479, 2806 |
| AlwI | GGATC | 1 cut(s) | 510 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1843 |
| Ama87I | CYCGRG | 2 cut(s) | 1144, 1952 |
| Aor13HI | TCCGGA | 1 cut(s) | 1324 |
| ApaI | GGGCCC | 1 cut(s) | 1425 |
| ApoI | RAATTY | 6 cut(s) | 49, 812, 838, 2013, 2089, 2577 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 1108, 1140, 1767, 1799 |
| AseI | ATTAAT | 1 cut(s) | 264 |
| AsiGI | ACCGGT | 1 cut(s) | 2229 |
| AspLEI | GCGC | 7 cut(s) | 7, 24, 1228, 1254, 1613, 2350, 2833 |
| AspS9I | GGNCC | 9 cut(s) | 211, 1124, 1322, 1421, 1422, 1435, 1537, 1950, 2494 |
| AsuC2I | CCSGG | 7 cut(s) | 297, 1397, 1584, 1940, 1953, 1954, 2729 |
| AsuHPI | GGTGA | 7 cut(s) | 214, 311, 437, 1543, 1564, 1577, 2526 |
| AvaI | CYCGRG | 2 cut(s) | 1144, 1952 |
| AvaII | GGWCC | 4 cut(s) | 1124, 1322, 1435, 2494 |
| BaeGI | GKGCMC | 2 cut(s) | 563, 1425 |
| BanI | GGYRCC | 2 cut(s) | 608, 1610 |
| BanII | GRGCYC | 1 cut(s) | 1425 |
| BauI | CACGAG | 1 cut(s) | 1616 |
| BbrPI | CACGTG | 2 cut(s) | 1296, 1428 |
| BbsI | GAAGAC | 1 cut(s) | 1855 |
| Bbv12I | GWGCWC | 4 cut(s) | 160, 1395, 2379, 2744 |
| BbvCI | CCTCAGC | 1 cut(s) | 498 |
| BccI | CCATC | 4 cut(s) | 326, 1472, 2078, 2320 |
| BceAI | ACGGC | 8 cut(s) | 89, 424, 649, 1096, 1294, 1332, 2058, 2090 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 765, 799, 1846, 1880 |
| BciT130I | CCWGG | 3 cut(s) | 1433, 2200, 2709 |
| BciVI | GTATCC | 1 cut(s) | 1283 |
| BclI | TGATCA | 3 cut(s) | 219, 331, 589 |
| BcnI | CCSGG | 7 cut(s) | 297, 1397, 1584, 1940, 1953, 1954, 2729 |
| BcoDI | GTCTC | 3 cut(s) | 932, 1479, 2806 |
| BcuI | ACTAGT | 1 cut(s) | 371 |
| BfaI | CTAG | 6 cut(s) | 8, 372, 444, 818, 1674, 2283 |
| BfmI | CTRYAG | 4 cut(s) | 1821, 2213, 2291, 2481 |
| BfoI | RGCGCY | 3 cut(s) | 8, 1614, 2351 |
| BfuAI | ACCTGC | 1 cut(s) | 1819 |
| BfuI | GTATCC | 1 cut(s) | 1283 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1420 |
| BmcAI | AGTACT | 3 cut(s) | 370, 400, 929 |
| Bme18I | GGWCC | 4 cut(s) | 1124, 1322, 1435, 2494 |
| BmeT110I | CYCGRG | 2 cut(s) | 1144, 1952 |
| BmgT120I | GGNCC | 9 cut(s) | 211, 1124, 1322, 1421, 1422, 1435, 1537, 1950, 2494 |
| BmiI | GGNNCC | 7 cut(s) | 610, 1125, 1323, 1422, 1423, 1437, 1612 |
| BmrI | ACTGGG | 1 cut(s) | 1558 |
| BmuI | ACTGGG | 1 cut(s) | 1558 |
| BpiI | GAAGAC | 1 cut(s) | 1855 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1127, 1159 |
| Bpu10I | CCTNAGC | 2 cut(s) | 207, 498 |
| BpuEI | CTTGAG | 2 cut(s) | 1764, 1817 |
| BpuMI | CCSGG | 7 cut(s) | 297, 1397, 1584, 1940, 1953, 1954, 2729 |
| Bsa29I | ATCGAT | 1 cut(s) | 1626 |
| BsaAI | YACGTR | 3 cut(s) | 1296, 1428, 2206 |
| BsaBI | GATNNNNATC | 1 cut(s) | 2330 |
| BsaHI | GRCGYC | 1 cut(s) | 1611 |
| BsaJI | CCNNGG | 3 cut(s) | 1898, 1952, 1953 |
| BsaWI | WCCGGW | 5 cut(s) | 1324, 1444, 1562, 2229, 2674 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1043, 1073 |
| Bse118I | RCCGGY | 2 cut(s) | 1410, 2229 |
| Bse1I | ACTGG | 9 cut(s) | 406, 1553, 1786, 2312, 2384, 2432, 2594, 2685, 2849 |
| Bse3DI | GCAATG | 3 cut(s) | 829, 952, 1108 |
| Bse8I | GATNNNNATC | 1 cut(s) | 2330 |
| BseAI | TCCGGA | 1 cut(s) | 1324 |
| BseBI | CCWGG | 3 cut(s) | 1433, 2200, 2709 |
| BseCI | ATCGAT | 1 cut(s) | 1626 |
| BseDI | CCNNGG | 3 cut(s) | 1898, 1952, 1953 |
| BseGI | GGATG | 4 cut(s) | 117, 2034, 2225, 2737 |
| BseJI | GATNNNNATC | 1 cut(s) | 2330 |
| BseMI | GCAATG | 3 cut(s) | 829, 952, 1108 |
| BseNI | ACTGG | 9 cut(s) | 406, 1553, 1786, 2312, 2384, 2432, 2594, 2685, 2849 |
| BseSI | GKGCMC | 2 cut(s) | 563, 1425 |
| BseX3I | CGGCCG | 2 cut(s) | 1412, 2100 |
| BseYI | CCCAGC | 2 cut(s) | 1044, 1853 |
| BsgI | GTGCAG | 1 cut(s) | 2019 |
| Bsh1236I | CGCG | 3 cut(s) | 22, 905, 1469 |
| Bsh1285I | CGRYCG | 4 cut(s) | 174, 1376, 1415, 2103 |
| BshNI | GGYRCC | 2 cut(s) | 608, 1610 |
| BshTI | ACCGGT | 1 cut(s) | 2229 |
| BshVI | ATCGAT | 1 cut(s) | 1626 |
| BsiEI | CGRYCG | 4 cut(s) | 174, 1376, 1415, 2103 |
| BsiHKAI | GWGCWC | 4 cut(s) | 160, 1395, 2379, 2744 |
| BsiHKCI | CYCGRG | 2 cut(s) | 1144, 1952 |
| BslFI | GGGAC | 8 cut(s) | 122, 1181, 1421, 1606, 1631, 1880, 2272, 2758 |
| BsmAI | GTCTC | 3 cut(s) | 932, 1479, 2806 |
| BsmBI | CGTCTC | 2 cut(s) | 932, 2806 |
| BsmFI | GGGAC | 8 cut(s) | 122, 1181, 1421, 1606, 1631, 1880, 2272, 2758 |
| BsmI | GAATGC | 1 cut(s) | 2826 |
| BsoBI | CYCGRG | 2 cut(s) | 1144, 1952 |
| Bsp120I | GGGCCC | 1 cut(s) | 1421 |
| Bsp1286I | GDGCHC | 6 cut(s) | 160, 563, 1395, 1425, 2379, 2744 |
| Bsp13I | TCCGGA | 1 cut(s) | 1324 |
| Bsp1407I | TGTACA | 1 cut(s) | 1620 |
| BspDI | ATCGAT | 1 cut(s) | 1626 |
| BspEI | TCCGGA | 1 cut(s) | 1324 |
| BspFNI | CGCG | 3 cut(s) | 22, 905, 1469 |
| BspHI | TCATGA | 1 cut(s) | 2511 |
| BspLI | GGNNCC | 7 cut(s) | 610, 1125, 1323, 1422, 1423, 1437, 1612 |
| BspMAI | CTGCAG | 3 cut(s) | 1825, 2295, 2485 |
| BspMI | ACCTGC | 1 cut(s) | 1819 |
| BspPI | GGATC | 1 cut(s) | 510 |
| BspT107I | GGYRCC | 2 cut(s) | 608, 1610 |
| BsrBI | CCGCTC | 1 cut(s) | 510 |
| BsrDI | GCAATG | 3 cut(s) | 829, 952, 1108 |
| BsrFI | RCCGGY | 2 cut(s) | 1410, 2229 |
| BsrGI | TGTACA | 1 cut(s) | 1620 |
| BsrI | ACTGG | 9 cut(s) | 406, 1553, 1786, 2312, 2384, 2432, 2594, 2685, 2849 |
| BssAI | RCCGGY | 2 cut(s) | 1410, 2229 |
| BssECI | CCNNGG | 3 cut(s) | 1898, 1952, 1953 |
| BssNI | GRCGYC | 1 cut(s) | 1611 |
| BssSI | CACGAG | 1 cut(s) | 1616 |
| BssT1I | CCWWGG | 1 cut(s) | 1898 |
| Bst2BI | CACGAG | 1 cut(s) | 1616 |
| Bst2UI | CCWGG | 3 cut(s) | 1433, 2200, 2709 |
| Bst4CI | ACNGT | 8 cut(s) | 67, 239, 921, 1177, 1271, 1338, 2114, 2800 |
| Bst6I | CTCTTC | 2 cut(s) | 1730, 2405 |
| BstACI | GRCGYC | 1 cut(s) | 1611 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 1519, 1820 |
| BstAUI | TGTACA | 1 cut(s) | 1620 |
| BstBAI | YACGTR | 3 cut(s) | 1296, 1428, 2206 |
| BstC8I | GCNNGC | 9 cut(s) | 958, 962, 1221, 1412, 1524, 1708, 2225, 2291, 2346 |
| BstEII | GGTNACC | 1 cut(s) | 2232 |
| BstF5I | GGATG | 4 cut(s) | 117, 2034, 2225, 2737 |
| BstFNI | CGCG | 3 cut(s) | 22, 905, 1469 |
| BstH2I | RGCGCY | 3 cut(s) | 8, 1614, 2351 |
| BstHHI | GCGC | 7 cut(s) | 7, 24, 1228, 1254, 1613, 2350, 2833 |
| BstMAI | GTCTC | 3 cut(s) | 932, 1479, 2806 |
| BstMCI | CGRYCG | 4 cut(s) | 174, 1376, 1415, 2103 |
| BstNI | CCWGG | 3 cut(s) | 1433, 2200, 2709 |
| BstNSI | RCATGY | 1 cut(s) | 2227 |
| BstPI | GGTNACC | 1 cut(s) | 2232 |
| BstSFI | CTRYAG | 4 cut(s) | 1821, 2213, 2291, 2481 |
| BstSLI | GKGCMC | 2 cut(s) | 563, 1425 |
| BstSNI | TACGTA | 1 cut(s) | 2206 |
| BstUI | CGCG | 3 cut(s) | 22, 905, 1469 |
| BstV2I | GAAGAC | 1 cut(s) | 1855 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 1432, 2307 |
| BstZI | CGGCCG | 2 cut(s) | 1412, 2100 |
| Bsu15I | ATCGAT | 1 cut(s) | 1626 |
| BsuI | GTATCC | 1 cut(s) | 1283 |
| BsuTUI | ATCGAT | 1 cut(s) | 1626 |
| BtgZI | GCGATG | 3 cut(s) | 828, 1450, 1639 |
| BtsCI | GGATG | 4 cut(s) | 117, 2034, 2225, 2737 |
| BtsI | GCAGTG | 1 cut(s) | 1405 |
| BtsIMutI | CAGTG | 7 cut(s) | 96, 244, 522, 1405, 2036, 2377, 2833 |
| BveI | ACCTGC | 1 cut(s) | 1819 |
| Cac8I | GCNNGC | 9 cut(s) | 958, 962, 1221, 1412, 1524, 1708, 2225, 2291, 2346 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1843 |
| CciI | TCATGA | 1 cut(s) | 2511 |
| CciNI | GCGGCCGC | 1 cut(s) | 2100 |
| CfoI | GCGC | 7 cut(s) | 7, 24, 1228, 1254, 1613, 2350, 2833 |
| Cfr10I | RCCGGY | 2 cut(s) | 1410, 2229 |
| Cfr13I | GGNCC | 9 cut(s) | 211, 1124, 1322, 1421, 1422, 1435, 1537, 1950, 2494 |
| Cfr9I | CCCGGG | 1 cut(s) | 1952 |
| ClaI | ATCGAT | 1 cut(s) | 1626 |
| CseI | GACGC | 2 cut(s) | 1225, 1603 |
| CspAI | ACCGGT | 1 cut(s) | 2229 |
| CviAII | CATG | 8 cut(s) | 1607, 1659, 1828, 2050, 2224, 2407, 2512, 2585 |
| DinI | GGCGCC | 1 cut(s) | 1612 |
| DraI | TTTAAA | 1 cut(s) | 286 |
| EaeI | YGGCCR | 5 cut(s) | 102, 1412, 1472, 2100, 2849 |
| EagI | CGGCCG | 2 cut(s) | 1412, 2100 |
| Eam1104I | CTCTTC | 2 cut(s) | 1730, 2405 |
| EarI | CTCTTC | 2 cut(s) | 1730, 2405 |
| EciI | GGCGGA | 3 cut(s) | 426, 1110, 1541 |
| EclXI | CGGCCG | 2 cut(s) | 1412, 2100 |
| Eco105I | TACGTA | 1 cut(s) | 2206 |
| Eco130I | CCWWGG | 1 cut(s) | 1898 |
| Eco24I | GRGCYC | 1 cut(s) | 1425 |
| Eco32I | GATATC | 2 cut(s) | 1721, 2008 |
| Eco47I | GGWCC | 4 cut(s) | 1124, 1322, 1435, 2494 |
| Eco52I | CGGCCG | 2 cut(s) | 1412, 2100 |
| Eco57I | CTGAAG | 1 cut(s) | 2510 |
| Eco72I | CACGTG | 2 cut(s) | 1296, 1428 |
| Eco88I | CYCGRG | 2 cut(s) | 1144, 1952 |
| Eco91I | GGTNACC | 1 cut(s) | 2232 |
| EcoO109I | RGGNCCY | 2 cut(s) | 1421, 2494 |
| EcoO65I | GGTNACC | 1 cut(s) | 2232 |
| EcoRI | GAATTC | 2 cut(s) | 49, 838 |
| EcoRII | CCWGG | 3 cut(s) | 1431, 2198, 2707 |
| EcoRV | GATATC | 2 cut(s) | 1721, 2008 |
| EcoT14I | CCWWGG | 1 cut(s) | 1898 |
| EcoT22I | ATGCAT | 1 cut(s) | 2225 |
| EcoT38I | GRGCYC | 1 cut(s) | 1425 |
| EgeI | GGCGCC | 1 cut(s) | 1612 |
| EheI | GGCGCC | 1 cut(s) | 1612 |
| ErhI | CCWWGG | 1 cut(s) | 1898 |
| Esp3I | CGTCTC | 2 cut(s) | 932, 2806 |
| FaeI | CATG | 8 cut(s) | 1610, 1662, 1831, 2053, 2227, 2410, 2515, 2588 |
| FaqI | GGGAC | 8 cut(s) | 122, 1181, 1421, 1606, 1631, 1880, 2272, 2758 |
| FatI | CATG | 8 cut(s) | 1606, 1658, 1827, 2049, 2223, 2406, 2511, 2584 |
| FauI | CCCGC | 4 cut(s) | 147, 1157, 1312, 1583 |
| FauNDI | CATATG | 2 cut(s) | 2637, 2860 |
| FbaI | TGATCA | 3 cut(s) | 219, 331, 589 |
| FblI | GTMKAC | 3 cut(s) | 1214, 1376, 2109 |
| FokI | GGATG | 4 cut(s) | 124, 2041, 2232, 2744 |
| FriOI | GRGCYC | 1 cut(s) | 1425 |
| FspBI | CTAG | 6 cut(s) | 8, 372, 444, 818, 1674, 2283 |
| FspI | TGCGCA | 1 cut(s) | 2832 |
| GlaI | GCGC | 7 cut(s) | 6, 23, 1227, 1253, 1612, 2349, 2832 |
| GsaI | CCCAGC | 2 cut(s) | 1048, 1857 |
| HaeII | RGCGCY | 3 cut(s) | 8, 1614, 2351 |
| HgaI | GACGC | 2 cut(s) | 1225, 1603 |
| HhaI | GCGC | 7 cut(s) | 7, 24, 1228, 1254, 1613, 2350, 2833 |
| Hin1I | GRCGYC | 1 cut(s) | 1611 |
| Hin1II | CATG | 8 cut(s) | 1610, 1662, 1831, 2053, 2227, 2410, 2515, 2588 |
| Hin6I | GCGC | 7 cut(s) | 5, 22, 1226, 1252, 1611, 2348, 2831 |
| HinP1I | GCGC | 7 cut(s) | 5, 22, 1226, 1252, 1611, 2348, 2831 |
| HincII | GTYRAC | 2 cut(s) | 1215, 1377 |
| HindII | GTYRAC | 2 cut(s) | 1215, 1377 |
| HindIII | AAGCTT | 1 cut(s) | 1708 |
| HphI | GGTGA | 7 cut(s) | 214, 311, 437, 1543, 1564, 1577, 2526 |
| Hpy166II | GTNNAC | 7 cut(s) | 1215, 1377, 1621, 1987, 2110, 2117, 2524 |
| Hpy188III | TCNNGA | 7 cut(s) | 132, 698, 1274, 1325, 1648, 2074, 2512 |
| Hpy8I | GTNNAC | 7 cut(s) | 1215, 1377, 1621, 1987, 2110, 2117, 2524 |
| Hpy99I | CGWCG | 8 cut(s) | 49, 248, 314, 410, 617, 659, 787, 1219 |
| HpyCH4III | ACNGT | 8 cut(s) | 67, 239, 921, 1177, 1271, 1338, 2114, 2800 |
| HpyCH4IV | ACGT | 8 cut(s) | 1295, 1311, 1427, 1689, 2205, 2386, 2436, 2526 |
| HpySE526I | ACGT | 8 cut(s) | 1295, 1311, 1427, 1689, 2205, 2386, 2436, 2526 |
| Hsp92I | GRCGYC | 1 cut(s) | 1611 |
| Hsp92II | CATG | 8 cut(s) | 1610, 1662, 1831, 2053, 2227, 2410, 2515, 2588 |
| HspAI | GCGC | 7 cut(s) | 5, 22, 1226, 1252, 1611, 2348, 2831 |
| KasI | GGCGCC | 1 cut(s) | 1610 |
| Kpn2I | TCCGGA | 1 cut(s) | 1324 |
| KroI | GCCGGC | 1 cut(s) | 1410 |
| KroNI | GCCGGC | 1 cut(s) | 1412 |
| Ksp22I | TGATCA | 3 cut(s) | 219, 331, 589 |
| LmnI | GCTCC | 2 cut(s) | 2324, 2677 |
| MaeI | CTAG | 6 cut(s) | 8, 372, 444, 818, 1674, 2283 |
| MaeII | ACGT | 8 cut(s) | 1295, 1311, 1427, 1689, 2205, 2386, 2436, 2526 |
| MaeIII | GTNAC | 6 cut(s) | 67, 239, 1312, 1958, 2232, 2396 |
| MbiI | CCGCTC | 1 cut(s) | 510 |
| MboII | GAAGA | 7 cut(s) | 1081, 1084, 1213, 1717, 1855, 2422, 2868 |
| MfeI | CAATTG | 2 cut(s) | 491, 2745 |
| MhlI | GDGCHC | 6 cut(s) | 160, 563, 1395, 1425, 2379, 2744 |
| Mly113I | GGCGCC | 1 cut(s) | 1611 |
| MlyI | GAGTC | 8 cut(s) | 144, 1052, 1145, 1196, 1205, 1902, 2290, 2744 |
| MmeI | TCCRAC | 4 cut(s) | 765, 1080, 1164, 1212 |
| Mph1103I | ATGCAT | 1 cut(s) | 2225 |
| MroI | TCCGGA | 1 cut(s) | 1324 |
| MroNI | GCCGGC | 1 cut(s) | 1410 |
| MseI | TTAA | 7 cut(s) | 14, 264, 285, 1160, 2312, 2499, 2881 |
| MslI | CAYNNNNRTG | 2 cut(s) | 89, 2405 |
| MspA1I | CMGCKG | 2 cut(s) | 337, 1089 |
| MunI | CAATTG | 2 cut(s) | 491, 2745 |
| Mva1269I | GAATGC | 1 cut(s) | 2826 |
| MvaI | CCWGG | 3 cut(s) | 1433, 2200, 2709 |
| MvnI | CGCG | 3 cut(s) | 22, 905, 1469 |
| NaeI | GCCGGC | 1 cut(s) | 1412 |
| NarI | GGCGCC | 1 cut(s) | 1611 |
| NciI | CCSGG | 7 cut(s) | 297, 1397, 1584, 1940, 1953, 1954, 2729 |
| NdeI | CATATG | 2 cut(s) | 2637, 2860 |
| NgoMIV | GCCGGC | 1 cut(s) | 1410 |
| NlaIII | CATG | 8 cut(s) | 1610, 1662, 1831, 2053, 2227, 2410, 2515, 2588 |
| NlaIV | GGNNCC | 7 cut(s) | 610, 1125, 1323, 1422, 1423, 1437, 1612 |
| NmuCI | GTSAC | 3 cut(s) | 67, 239, 1312 |
| NotI | GCGGCCGC | 1 cut(s) | 2100 |
| NsbI | TGCGCA | 1 cut(s) | 2832 |
| NsiI | ATGCAT | 1 cut(s) | 2225 |
| NspI | RCATGY | 1 cut(s) | 2227 |
| PaeI | GCATGC | 1 cut(s) | 2227 |
| PagI | TCATGA | 1 cut(s) | 2511 |
| PcsI | WCGNNNNNNNCGW | 6 cut(s) | 621, 938, 1196, 1205, 1623, 1757 |
| PctI | GAATGC | 1 cut(s) | 2826 |
| PdiI | GCCGGC | 1 cut(s) | 1412 |
| PfeI | GAWTC | 9 cut(s) | 572, 851, 980, 1151, 1262, 2331, 2531, 2597, 2873 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 1470, 2055 |
| PfoI | TCCNGGA | 1 cut(s) | 2727 |
| PinAI | ACCGGT | 1 cut(s) | 2229 |
| PleI | GAGTC | 8 cut(s) | 143, 1052, 1144, 1195, 1204, 1901, 2290, 2743 |
| PluTI | GGCGCC | 1 cut(s) | 1614 |
| PmaCI | CACGTG | 2 cut(s) | 1296, 1428 |
| PmlI | CACGTG | 2 cut(s) | 1296, 1428 |
| PpsI | GAGTC | 8 cut(s) | 143, 1052, 1144, 1195, 1204, 1901, 2290, 2743 |
| Ppu21I | YACGTR | 3 cut(s) | 1296, 1428, 2206 |
| PpuMI | RGGWCCY | 1 cut(s) | 2494 |
| PshBI | ATTAAT | 1 cut(s) | 264 |
| Psp5II | RGGWCCY | 1 cut(s) | 2494 |
| Psp6I | CCWGG | 3 cut(s) | 1431, 2198, 2707 |
| PspCI | CACGTG | 2 cut(s) | 1296, 1428 |
| PspEI | GGTNACC | 1 cut(s) | 2232 |
| PspFI | CCCAGC | 2 cut(s) | 1044, 1853 |
| PspGI | CCWGG | 3 cut(s) | 1431, 2198, 2707 |
| PspN4I | GGNNCC | 7 cut(s) | 610, 1125, 1323, 1422, 1423, 1437, 1612 |
| PspOMI | GGGCCC | 1 cut(s) | 1421 |
| PspPI | GGNCC | 9 cut(s) | 211, 1124, 1322, 1421, 1422, 1435, 1537, 1950, 2494 |
| PspPPI | RGGWCCY | 1 cut(s) | 2494 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 358, 390 |
| PstI | CTGCAG | 3 cut(s) | 1825, 2295, 2485 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1843 |
| PvuII | CAGCTG | 1 cut(s) | 337 |
| RseI | CAYNNNNRTG | 2 cut(s) | 89, 2405 |
| SalI | GTCGAC | 2 cut(s) | 1213, 1375 |
| SaqAI | TTAA | 7 cut(s) | 14, 264, 285, 1160, 2312, 2499, 2881 |
| Sau96I | GGNCC | 9 cut(s) | 211, 1124, 1322, 1421, 1422, 1435, 1537, 1950, 2494 |
| ScaI | AGTACT | 3 cut(s) | 370, 400, 929 |
| SchI | GAGTC | 8 cut(s) | 144, 1052, 1145, 1196, 1205, 1902, 2290, 2744 |
| SduI | GDGCHC | 6 cut(s) | 160, 563, 1395, 1425, 2379, 2744 |
| SfcI | CTRYAG | 4 cut(s) | 1821, 2213, 2291, 2481 |
| SfiI | GGCCNNNNNGGCC | 1 cut(s) | 1420 |
| SfoI | GGCGCC | 1 cut(s) | 1612 |
| SinI | GGWCC | 4 cut(s) | 1124, 1322, 1435, 2494 |
| SmaI | CCCGGG | 1 cut(s) | 1954 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 89, 2405 |
| SmlI | CTYRAG | 2 cut(s) | 1779, 1796 |
| SmoI | CTYRAG | 2 cut(s) | 1779, 1796 |
| SnaBI | TACGTA | 1 cut(s) | 2206 |
| SpeI | ACTAGT | 1 cut(s) | 371 |
| SphI | GCATGC | 1 cut(s) | 2227 |
| SspDI | GGCGCC | 1 cut(s) | 1610 |
| SspI | AATATT | 2 cut(s) | 262, 727 |
| SspMI | CTAG | 6 cut(s) | 8, 372, 444, 818, 1674, 2283 |
| StyI | CCWWGG | 1 cut(s) | 1898 |
| TaaI | ACNGT | 8 cut(s) | 67, 239, 921, 1177, 1271, 1338, 2114, 2800 |
| TaiI | ACGT | 8 cut(s) | 1298, 1314, 1430, 1692, 2208, 2389, 2439, 2529 |
| TatI | WGTACW | 5 cut(s) | 368, 398, 927, 967, 1620 |
| TfiI | GAWTC | 9 cut(s) | 572, 851, 980, 1151, 1262, 2331, 2531, 2597, 2873 |
| Tru1I | TTAA | 7 cut(s) | 14, 264, 285, 1160, 2312, 2499, 2881 |
| Tru9I | TTAA | 7 cut(s) | 14, 264, 285, 1160, 2312, 2499, 2881 |
| TscAI | CASTG | 7 cut(s) | 96, 244, 522, 1412, 2043, 2384, 2833 |
| TseFI | GTSAC | 3 cut(s) | 67, 239, 1312 |
| Tsp45I | GTSAC | 3 cut(s) | 67, 239, 1312 |
| TspGWI | ACGGA | 1 cut(s) | 1302 |
| TspMI | CCCGGG | 1 cut(s) | 1952 |
| TspRI | CASTG | 7 cut(s) | 96, 244, 522, 1412, 2043, 2384, 2833 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 1470, 2055 |
| VpaK11BI | GGWCC | 4 cut(s) | 1124, 1322, 1435, 2494 |
| VspI | ATTAAT | 1 cut(s) | 264 |
| XapI | RAATTY | 6 cut(s) | 49, 812, 838, 2013, 2089, 2577 |
| XceI | RCATGY | 1 cut(s) | 2227 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 398 |
| XmaI | CCCGGG | 1 cut(s) | 1952 |
| XmiI | GTMKAC | 3 cut(s) | 1214, 1376, 2109 |
| XspI | CTAG | 6 cut(s) | 8, 372, 444, 818, 1674, 2283 |
| ZrmI | AGTACT | 3 cut(s) | 370, 400, 929 |
| Zsp2I | ATGCAT | 1 cut(s) | 2225 |