pycom15g23010

U1 small nuclear ribonucleoprotein 70

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009950
Physical Location & Seq
Reverse (-)
17089865 .. 17091821
1957 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 516 bp
ATGGGAGACTTCATCAACGATGCCTTCATGCGCGGCCAAAACGCCGCCGTTCAGAGCCGACCCAAGGCCCACAACCGCCCAAATGTTGTTCAGCTCAAAATGATTGGGCAGAGCCACCCCACTGGTCTAACAACAAACCTTTTGAAGCTGTTTGAGCCTAGACCGCCGTTGGATTTCAAACCGCCTCCGGAGAAGAAAAAGTGCCCACCTTTGACAGGGGTAGCGCAATTTGTGAGTAAGTTTGCGGAGCCCGGAGATCCCGAGTATAATCCGCCTATCCAAAAGGGTGAAACTCCTACACAAAGAAGGGCTCGAATACACCAGTTAAGACTTGAGAAGGGTGCAGCAAAGGCTGCGGAAGAGCTAGAGAAATATGATCCAAATAATGATCCGAGTATTTCTGGAGATCCATACAAGACTTTGTTTGTTGCTAGACTCAATTACGAGACAACTGAGAGCAGAATCAAAAGGGAGTTTGATGTTTATGGTCCAATCAAGCGGGTAGGTATTTTTTGA

Protein Analysis

172

Amino Acids

19.3

Weight (kDa)

9.54

Isoelectric Point (pI)

37.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U1snRNP70_N PF12220 42 - 130 9.3e-21 U1 small nuclear ribonucleoprotein of 70kDa MW N terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 33
AccIII TCCGGA 1 cut(s) 187
AciI CCGC 9 cut(s) 33, 45, 76, 164, 182, 245, 272, 356, 499
AclWI GGATC 4 cut(s) 251, 371, 383, 401
AcoI YGGCCR 1 cut(s) 34
AfiI CCNNNNNNNGG 2 cut(s) 64, 215
AgsI TTSAA 2 cut(s) 145, 178
AluBI AGCT 3 cut(s) 94, 148, 364
AluI AGCT 3 cut(s) 94, 148, 364
Alw26I GTCTC 1 cut(s) 440
AlwI GGATC 4 cut(s) 251, 371, 383, 401
Ama87I CYCGRG 1 cut(s) 260
Aor13HI TCCGGA 1 cut(s) 187
AoxI GGCC 2 cut(s) 34, 66
ApeKI GCWGC 2 cut(s) 344, 353
AspLEI GCGC 2 cut(s) 33, 226
AspS9I GGNCC 2 cut(s) 67, 488
AsuC2I CCSGG 1 cut(s) 252
AsuHPI GGTGA 1 cut(s) 299
AvaI CYCGRG 1 cut(s) 260
AvaII GGWCC 1 cut(s) 488
BaeGI GKGCMC 1 cut(s) 206
BanII GRGCYC 2 cut(s) 252, 313
BbvI GCAGC 2 cut(s) 340, 356
BceAI ACGGC 2 cut(s) 32, 151
BcnI CCSGG 1 cut(s) 252
BcoDI GTCTC 1 cut(s) 440
BfaI CTAG 3 cut(s) 159, 365, 432
BisI GCNGC 4 cut(s) 34, 45, 345, 354
BlsI GCNGC 4 cut(s) 35, 46, 346, 355
Bme1390I CCNGG 1 cut(s) 252
Bme18I GGWCC 1 cut(s) 488
BmeT110I CYCGRG 1 cut(s) 260
BmgT120I GGNCC 2 cut(s) 67, 488
BmiI GGNNCC 1 cut(s) 249
BmrFI CCNGG 1 cut(s) 252
BmsI GCATC 1 cut(s) 10
BpmI CTGGAG 1 cut(s) 423
BpuEI CTTGAG 1 cut(s) 353
BpuMI CCSGG 1 cut(s) 252
BsaJI CCNNGG 1 cut(s) 63
BsaWI WCCGGW 1 cut(s) 187
Bsc4I CCNNNNNNNGG 2 cut(s) 64, 215
Bse1I ACTGG 2 cut(s) 127, 322
BseAI TCCGGA 1 cut(s) 187
BseDI CCNNGG 1 cut(s) 63
BseLI CCNNNNNNNGG 2 cut(s) 64, 215
BseMII CTCAG 1 cut(s) 444
BseNI ACTGG 2 cut(s) 127, 322
BseSI GKGCMC 1 cut(s) 206
BseXI GCAGC 2 cut(s) 340, 356
BsgI GTGCAG 1 cut(s) 363
Bsh1236I CGCG 1 cut(s) 33
BshFI GGCC 2 cut(s) 36, 68
BsiHKCI CYCGRG 1 cut(s) 260
BsiSI CCGG 2 cut(s) 188, 252
BslI CCNNNNNNNGG 2 cut(s) 64, 215
BsmAI GTCTC 1 cut(s) 440
BsnI GGCC 2 cut(s) 36, 68
BsoBI CYCGRG 1 cut(s) 260
Bsp1286I GDGCHC 3 cut(s) 206, 252, 313
Bsp13I TCCGGA 1 cut(s) 187
Bsp143I GATC 4 cut(s) 256, 376, 388, 406
BspACI CCGC 9 cut(s) 33, 45, 76, 164, 182, 245, 272, 356, 499
BspANI GGCC 2 cut(s) 36, 68
BspCNI CTCAG 1 cut(s) 445
BspEI TCCGGA 1 cut(s) 187
BspFNI CGCG 1 cut(s) 33
BspLI GGNNCC 1 cut(s) 249
BspPI GGATC 4 cut(s) 251, 371, 383, 401
BspQI GCTCTTC 1 cut(s) 354
BsrI ACTGG 2 cut(s) 127, 322
BssECI CCNNGG 1 cut(s) 63
BssMI GATC 4 cut(s) 256, 376, 388, 406
BssT1I CCWWGG 1 cut(s) 63
Bst6I CTCTTC 1 cut(s) 354
BstAPI GCANNNNNTGC 1 cut(s) 353
BstDEI CTNAG 1 cut(s) 453
BstENI CCTNNNNNAGG 1 cut(s) 213
BstFNI CGCG 1 cut(s) 33
BstHHI GCGC 2 cut(s) 33, 226
BstKTI GATC 4 cut(s) 259, 379, 391, 409
BstMAI GTCTC 1 cut(s) 440
BstMBI GATC 4 cut(s) 256, 376, 388, 406
BstMWI GCNNNNNNNGC 4 cut(s) 154, 163, 350, 353
BstSCI CCNGG 1 cut(s) 250
BstSLI GKGCMC 1 cut(s) 206
BstUI CGCG 1 cut(s) 33
BstV1I GCAGC 2 cut(s) 340, 356
BstX2I RGATCY 2 cut(s) 256, 406
BstXI CCANNNNNNTGG 1 cut(s) 122
BstYI RGATCY 2 cut(s) 256, 406
BsuRI GGCC 2 cut(s) 36, 68
BtsIMutI CAGTG 1 cut(s) 120
CfoI GCGC 2 cut(s) 33, 226
Cfr13I GGNCC 2 cut(s) 67, 488
CviAII CATG 1 cut(s) 28
DdeI CTNAG 1 cut(s) 453
DpnI GATC 4 cut(s) 258, 378, 390, 408
DpnII GATC 4 cut(s) 256, 376, 388, 406
EaeI YGGCCR 1 cut(s) 34
Eam1104I CTCTTC 1 cut(s) 354
EarI CTCTTC 1 cut(s) 354
EciI GGCGGA 1 cut(s) 261
Eco130I CCWWGG 1 cut(s) 63
Eco24I GRGCYC 2 cut(s) 252, 313
Eco47I GGWCC 1 cut(s) 488
Eco88I CYCGRG 1 cut(s) 260
EcoNI CCTNNNNNAGG 1 cut(s) 213
EcoT14I CCWWGG 1 cut(s) 63
EcoT38I GRGCYC 2 cut(s) 252, 313
ErhI CCWWGG 1 cut(s) 63
FaeI CATG 1 cut(s) 31
FaiI YATR 5 cut(s) 29, 267, 375, 412, 486
FatI CATG 1 cut(s) 27
FauI CCCGC 1 cut(s) 492
Fnu4HI GCNGC 4 cut(s) 34, 45, 345, 354
FriOI GRGCYC 2 cut(s) 252, 313
Fsp4HI GCNGC 4 cut(s) 34, 45, 345, 354
FspBI CTAG 3 cut(s) 159, 365, 432
GlaI GCGC 2 cut(s) 32, 225
GluI GCNGC 4 cut(s) 34, 45, 345, 354
GsuI CTGGAG 1 cut(s) 423
HaeIII GGCC 2 cut(s) 36, 68
HapII CCGG 2 cut(s) 188, 252
HhaI GCGC 2 cut(s) 33, 226
Hin1II CATG 1 cut(s) 31
Hin6I GCGC 2 cut(s) 31, 224
HinP1I GCGC 2 cut(s) 31, 224
HinfI GANTC 2 cut(s) 435, 462
HpaII CCGG 2 cut(s) 188, 252
HphI GGTGA 1 cut(s) 299
Hpy188I TCNGA 2 cut(s) 54, 393
Hpy188III TCNNGA 3 cut(s) 188, 260, 402
HpyAV CCTTC 3 cut(s) 34, 300, 331
HpyCH4V TGCA 1 cut(s) 344
HpyF10VI GCNNNNNNNGC 4 cut(s) 154, 163, 350, 353
HpyF3I CTNAG 1 cut(s) 453
Hsp92II CATG 1 cut(s) 31
HspAI GCGC 2 cut(s) 31, 224
Kpn2I TCCGGA 1 cut(s) 187
Kzo9I GATC 4 cut(s) 256, 376, 388, 406
LguI GCTCTTC 1 cut(s) 354
LmnI GCTCC 1 cut(s) 247
LpnPI CCDG 6 cut(s) 108, 201, 201, 265, 335, 387
Lsp1109I GCAGC 2 cut(s) 340, 356
LweI GCATC 1 cut(s) 10
MaeI CTAG 3 cut(s) 159, 365, 432
MalI GATC 4 cut(s) 258, 378, 390, 408
MboI GATC 4 cut(s) 256, 376, 388, 406
MboII GAAGA 2 cut(s) 205, 371
MflI RGATCY 2 cut(s) 256, 406
MhlI GDGCHC 3 cut(s) 206, 252, 313
MluCI AATT 2 cut(s) 227, 439
MlyI GAGTC 1 cut(s) 429
MmeI TCCRAC 1 cut(s) 150
MnlI CCTC 1 cut(s) 195
MroI TCCGGA 1 cut(s) 187
MseI TTAA 1 cut(s) 326
MspI CCGG 2 cut(s) 188, 252
MspR9I CCNGG 1 cut(s) 252
MvnI CGCG 1 cut(s) 33
MwoI GCNNNNNNNGC 4 cut(s) 154, 163, 350, 353
NciI CCSGG 1 cut(s) 252
NdeII GATC 4 cut(s) 256, 376, 388, 406
NlaIII CATG 1 cut(s) 31
NlaIV GGNNCC 1 cut(s) 249
PciSI GCTCTTC 1 cut(s) 354
PfeI GAWTC 1 cut(s) 462
PkrI GCNGC 4 cut(s) 35, 46, 346, 355
PleI GAGTC 1 cut(s) 429
PpsI GAGTC 1 cut(s) 429
PspN4I GGNNCC 1 cut(s) 249
PspPI GGNCC 2 cut(s) 67, 488
PsuI RGATCY 2 cut(s) 256, 406
SapI GCTCTTC 1 cut(s) 354
SaqAI TTAA 1 cut(s) 326
SatI GCNGC 4 cut(s) 34, 45, 345, 354
Sau3AI GATC 4 cut(s) 256, 376, 388, 406
Sau96I GGNCC 2 cut(s) 67, 488
SchI GAGTC 1 cut(s) 429
ScrFI CCNGG 1 cut(s) 252
SduI GDGCHC 3 cut(s) 206, 252, 313
SetI ASST 6 cut(s) 96, 141, 150, 211, 366, 508
SfaNI GCATC 1 cut(s) 10
SinI GGWCC 1 cut(s) 488
SmlI CTYRAG 1 cut(s) 332
SmoI CTYRAG 1 cut(s) 332
Sse9I AATT 2 cut(s) 227, 439
SsiI CCGC 9 cut(s) 33, 45, 76, 164, 182, 245, 272, 356, 499
SspMI CTAG 3 cut(s) 159, 365, 432
StyD4I CCNGG 1 cut(s) 250
StyI CCWWGG 1 cut(s) 63
TaqI TCGA 1 cut(s) 313
TasI AATT 2 cut(s) 227, 439
TauI GCSGC 2 cut(s) 36, 47
TfiI GAWTC 1 cut(s) 462
Tru1I TTAA 1 cut(s) 326
Tru9I TTAA 1 cut(s) 326
TscAI CASTG 1 cut(s) 127
TseI GCWGC 2 cut(s) 344, 353
TspDTI ATGAA 1 cut(s) 16
TspRI CASTG 1 cut(s) 127
VpaK11BI GGWCC 1 cut(s) 488
XagI CCTNNNNNAGG 1 cut(s) 213
XspI CTAG 3 cut(s) 159, 365, 432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.