pycom15g23620

Cold-regulated 413 plasma membrane protein 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0014958
Physical Location & Seq
Reverse (-)
17773318 .. 17774210
893 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 393 bp
ATGCTGACTTCGCTTTTAATCCCTTACATTTTCTTCAGTCTTCCTTCAGTGCTGTTCGACCTCCTAAGAGGAGAGGTTGGAAGATGGATTGCTTTTGTTGCCGTTGTACTGAGGCTTTTCTTCCCAAGGCATTTCCCAGATTGGCTAGAGATGCCGGGATCGTTGATCCTTCTTCTGGTTGTGGCTCCAAACTTGTTTGCGCACACGTTGAAGGACAGCTGGGTTGGCGTCTCGATCTGTCTTCTCATCGGTTGCTACCTGCTGCAAGAGCACATCCGGGCATCAGGTGGATTCCGAAATTCTTTTACACAGAGCCATGGTATATCGAACACGCTCGGAATCATCCTTCTCCTGGTCTACCCTGTCTGGGCATTGGTTCTCCATTTCATCTAA

Protein Analysis

131

Amino Acids

14.69

Weight (kDa)

7.89

Isoelectric Point (pI)

38.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WCOR413 PF05562 1 - 117 5.1e-63 Cold acclimation protein WCOR413
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014958)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50830
fragaria_vesca FvH4_1g14460
malus_domestica MD15G1273000.v1.1
prunus_persica Prupe.7G145800_v2.0.a1 Prupe.7G145800_v2.0.a1
pyrus_communis pycom15g23620
rosa_chinensis RchiOBHm_Chr2g0103221
rosa_laevigata RLG00000017215
rosa_roxburghii Rroxscaffold_2G00139860
rosa_rugosa Rorug02G0112300 Rorug02G0112400
rosa_samantha Rh2AG161100 Rh2BG167900 Rh2CG168100 Rh2DG166800
rosa_wichuraiana Rw2G012640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 201
Acc36I ACCTGC 1 cut(s) 267
AccI GTMKAC 1 cut(s) 357
AclWI GGATC 2 cut(s) 160, 166
AcsI RAATTY 1 cut(s) 298
AcuI CTGAAG 2 cut(s) 19, 30
AcyI GRCGYC 1 cut(s) 228
AfaI GTAC 1 cut(s) 108
AfiI CCNNNNNNNGG 3 cut(s) 175, 352, 367
AflIII ACRYGT 1 cut(s) 204
AgsI TTSAA 1 cut(s) 211
AjnI CCWGG 1 cut(s) 351
AluBI AGCT 1 cut(s) 219
AluI AGCT 1 cut(s) 219
Alw21I GWGCWC 1 cut(s) 273
Alw26I GTCTC 1 cut(s) 235
AlwI GGATC 2 cut(s) 160, 166
ApeKI GCWGC 1 cut(s) 262
ApoI RAATTY 1 cut(s) 298
AspLEI GCGC 1 cut(s) 202
AsuC2I CCSGG 2 cut(s) 156, 278
BbsI GAAGAC 2 cut(s) 32, 233
Bbv12I GWGCWC 1 cut(s) 273
BbvI GCAGC 1 cut(s) 249
BccI CCATC 1 cut(s) 78
BceAI ACGGC 1 cut(s) 86
BciT130I CCWGG 1 cut(s) 353
BcnI CCSGG 2 cut(s) 156, 278
BcoDI GTCTC 1 cut(s) 235
BfaI CTAG 1 cut(s) 146
BfuAI ACCTGC 1 cut(s) 267
BisI GCNGC 1 cut(s) 263
BlsI GCNGC 1 cut(s) 264
Bme1390I CCNGG 3 cut(s) 156, 278, 353
BmiI GGNNCC 1 cut(s) 186
BmrFI CCNGG 3 cut(s) 156, 278, 353
BmsI GCATC 2 cut(s) 141, 290
BpiI GAAGAC 2 cut(s) 32, 233
BpuMI CCSGG 2 cut(s) 156, 278
BsaHI GRCGYC 1 cut(s) 228
BsaJI CCNNGG 2 cut(s) 125, 316
Bsc4I CCNNNNNNNGG 3 cut(s) 175, 352, 367
BseBI CCWGG 1 cut(s) 353
BseDI CCNNGG 2 cut(s) 125, 316
BseGI GGATG 2 cut(s) 273, 342
BseLI CCNNNNNNNGG 3 cut(s) 175, 352, 367
BseMII CTCAG 1 cut(s) 101
BseRI GAGGAG 1 cut(s) 84
BseXI GCAGC 1 cut(s) 249
BseYI CCCAGC 1 cut(s) 219
BsiHKAI GWGCWC 1 cut(s) 273
BsiSI CCGG 2 cut(s) 155, 277
BslI CCNNNNNNNGG 3 cut(s) 175, 352, 367
BsmAI GTCTC 1 cut(s) 235
BsmBI CGTCTC 1 cut(s) 235
Bsp1286I GDGCHC 1 cut(s) 273
Bsp143I GATC 3 cut(s) 158, 165, 234
Bsp19I CCATGG 1 cut(s) 316
BspCNI CTCAG 1 cut(s) 102
BspLI GGNNCC 1 cut(s) 186
BspMI ACCTGC 1 cut(s) 267
BspPI GGATC 2 cut(s) 160, 166
BssECI CCNNGG 2 cut(s) 125, 316
BssMI GATC 3 cut(s) 158, 165, 234
BssNI GRCGYC 1 cut(s) 228
BssT1I CCWWGG 2 cut(s) 125, 316
Bst2UI CCWGG 1 cut(s) 353
BstACI GRCGYC 1 cut(s) 228
BstDEI CTNAG 2 cut(s) 65, 110
BstDSI CCRYGG 1 cut(s) 316
BstF5I GGATG 2 cut(s) 273, 342
BstHHI GCGC 1 cut(s) 202
BstKTI GATC 3 cut(s) 161, 168, 237
BstMAI GTCTC 1 cut(s) 235
BstMBI GATC 3 cut(s) 158, 165, 234
BstMWI GCNNNNNNNGC 5 cut(s) 10, 98, 151, 225, 268
BstNI CCWGG 1 cut(s) 353
BstSCI CCNGG 3 cut(s) 154, 276, 351
BstV1I GCAGC 1 cut(s) 249
BstV2I GAAGAC 2 cut(s) 32, 233
BtgI CCRYGG 1 cut(s) 316
BtsCI GGATG 2 cut(s) 273, 342
BtsIMutI CAGTG 1 cut(s) 54
BveI ACCTGC 1 cut(s) 267
CfoI GCGC 1 cut(s) 202
CseI GACGC 1 cut(s) 217
Csp6I GTAC 1 cut(s) 107
CviAII CATG 1 cut(s) 317
CviJI RGCY 5 cut(s) 115, 145, 185, 219, 315
CviKI_1 RGCY 5 cut(s) 115, 145, 185, 219, 315
CviQI GTAC 1 cut(s) 107
DdeI CTNAG 2 cut(s) 65, 110
DpnI GATC 3 cut(s) 160, 167, 236
DpnII GATC 3 cut(s) 158, 165, 234
Eco130I CCWWGG 2 cut(s) 125, 316
Eco57I CTGAAG 2 cut(s) 19, 30
EcoRII CCWGG 1 cut(s) 351
EcoT14I CCWWGG 2 cut(s) 125, 316
ErhI CCWWGG 2 cut(s) 125, 316
Esp3I CGTCTC 1 cut(s) 235
FaeI CATG 1 cut(s) 320
FaiI YATR 2 cut(s) 318, 323
FatI CATG 1 cut(s) 316
FblI GTMKAC 1 cut(s) 357
Fnu4HI GCNGC 1 cut(s) 263
FokI GGATG 2 cut(s) 260, 329
Fsp4HI GCNGC 1 cut(s) 263
FspBI CTAG 1 cut(s) 146
FspI TGCGCA 1 cut(s) 201
GlaI GCGC 1 cut(s) 201
GluI GCNGC 1 cut(s) 263
GsaI CCCAGC 1 cut(s) 223
HapII CCGG 2 cut(s) 155, 277
HgaI GACGC 1 cut(s) 217
HhaI GCGC 1 cut(s) 202
Hin1I GRCGYC 1 cut(s) 228
Hin1II CATG 1 cut(s) 320
Hin6I GCGC 1 cut(s) 200
HinP1I GCGC 1 cut(s) 200
HinfI GANTC 2 cut(s) 291, 339
HpaII CCGG 2 cut(s) 155, 277
Hpy166II GTNNAC 1 cut(s) 358
Hpy188I TCNGA 2 cut(s) 296, 338
Hpy188III TCNNGA 1 cut(s) 232
Hpy8I GTNNAC 1 cut(s) 358
HpyAV CCTTC 4 cut(s) 54, 179, 205, 356
HpyCH4IV ACGT 1 cut(s) 206
HpyCH4V TGCA 1 cut(s) 265
HpyF10VI GCNNNNNNNGC 5 cut(s) 10, 98, 151, 225, 268
HpyF3I CTNAG 2 cut(s) 65, 110
HpySE526I ACGT 1 cut(s) 206
Hsp92I GRCGYC 1 cut(s) 228
Hsp92II CATG 1 cut(s) 320
HspAI GCGC 1 cut(s) 200
Kzo9I GATC 3 cut(s) 158, 165, 234
LmnI GCTCC 1 cut(s) 190
Lsp1109I GCAGC 1 cut(s) 249
LweI GCATC 2 cut(s) 141, 290
MaeI CTAG 1 cut(s) 146
MaeII ACGT 1 cut(s) 206
MalI GATC 3 cut(s) 160, 167, 236
MboI GATC 3 cut(s) 158, 165, 234
MboII GAAGA 6 cut(s) 25, 32, 93, 112, 164, 233
MhlI GDGCHC 1 cut(s) 273
MluCI AATT 1 cut(s) 298
MmeI TCCRAC 1 cut(s) 58
MnlI CCTC 4 cut(s) 62, 67, 71, 105
MseI TTAA 1 cut(s) 17
MspA1I CMGCKG 1 cut(s) 219
MspI CCGG 2 cut(s) 155, 277
MspR9I CCNGG 3 cut(s) 156, 278, 353
MvaI CCWGG 1 cut(s) 353
MwoI GCNNNNNNNGC 5 cut(s) 10, 98, 151, 225, 268
NciI CCSGG 2 cut(s) 156, 278
NcoI CCATGG 1 cut(s) 316
NdeII GATC 3 cut(s) 158, 165, 234
NlaIII CATG 1 cut(s) 320
NlaIV GGNNCC 1 cut(s) 186
NsbI TGCGCA 1 cut(s) 201
PfeI GAWTC 2 cut(s) 291, 339
PkrI GCNGC 1 cut(s) 264
Psp6I CCWGG 1 cut(s) 351
PspFI CCCAGC 1 cut(s) 219
PspGI CCWGG 1 cut(s) 351
PspN4I GGNNCC 1 cut(s) 186
PvuII CAGCTG 1 cut(s) 219
RsaI GTAC 1 cut(s) 108
RsaNI GTAC 1 cut(s) 107
SaqAI TTAA 1 cut(s) 17
SatI GCNGC 1 cut(s) 263
Sau3AI GATC 3 cut(s) 158, 165, 234
ScrFI CCNGG 3 cut(s) 156, 278, 353
SduI GDGCHC 1 cut(s) 273
SetI ASST 6 cut(s) 63, 78, 209, 221, 261, 289
SfaNI GCATC 2 cut(s) 141, 290
Sse9I AATT 1 cut(s) 298
SspMI CTAG 1 cut(s) 146
StyD4I CCNGG 3 cut(s) 154, 276, 351
StyI CCWWGG 2 cut(s) 125, 316
TaiI ACGT 1 cut(s) 209
TaqI TCGA 3 cut(s) 57, 233, 326
TasI AATT 1 cut(s) 298
TatI WGTACW 1 cut(s) 106
TfiI GAWTC 2 cut(s) 291, 339
Tru1I TTAA 1 cut(s) 17
Tru9I TTAA 1 cut(s) 17
TscAI CASTG 1 cut(s) 54
TseI GCWGC 1 cut(s) 262
TspDTI ATGAA 1 cut(s) 376
TspRI CASTG 1 cut(s) 54
XapI RAATTY 1 cut(s) 298
XmiI GTMKAC 1 cut(s) 357
XspI CTAG 1 cut(s) 146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.