RLG00000017215

Cold-regulated 413 plasma membrane protein 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0014958
Physical Location & Seq
Reverse (-)
14201890 .. 14204370
2481 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017215

Sequence Viewer

Length: 696 bp
ATGGGGAGGGTGGACTATTTGGCTATGAAGACTGACCCAGTTGCGGCCACCGACTTGATGAACTCTGATATCAATGAGCTTAAGATTGCGGCCAAGAGTCTCGTTAGGGATGTTATTAGGCTCGGTGGTCTTGGTTTTGGGACTACTTTTCTCAAATGGCTTGCCTCCTTTGCTGCCATATATTTGTTGGTATTGGATCGAACAAACTGGAGAACAAATATGCTGACTTCACTCTTAGTTCCTTATATTTTCTTTAGTCTTCCTTGGGGGCTGTTCCACTTATTGAGAGGAGAGGTTGGAAGCTGGATTGCTTTTGTTGCTGTTGTATTAAGGCTTTTCTGCCCGAGACATTTCCCAGATTGGCTGGAGATGCCGGGTTCTCTGATCCTTCTTCTGGTTGTGGCTCCAAGCTTCTTCGCAGAAACCTTGAAGGAGAGCTGGGTTGGTGTCCTGATATGTCTCCTCATTGGATGTTACCTGCTGCAAGAGCACATCCGGTCGTCTGGTGGATTCAGAAATTCATTCACACAAAGCCATGGAATATCAAACACTCTCGTTCAAAATTCGGTTTCTGCTTCTGCCCCGAAATTTGAACATGCAAAGACTGGAATATGGAATGGAACCTTGTTTGTGACTGAGGCTCTTCATTTATATCCTCAAGAGCACTCCAAGCAACATATTTTTGGACAGTCATAG

Protein Analysis

232

Amino Acids

25.86

Weight (kDa)

8.47

Isoelectric Point (pI)

31.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WCOR413 PF05562 10 - 185 6.8e-84 Cold acclimation protein WCOR413
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014958)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50830
fragaria_vesca FvH4_1g14460
malus_domestica MD15G1273000.v1.1
prunus_persica Prupe.7G145800_v2.0.a1 Prupe.7G145800_v2.0.a1
pyrus_communis pycom15g23620
rosa_chinensis RchiOBHm_Chr2g0103221
rosa_laevigata RLG00000017215
rosa_roxburghii Rroxscaffold_2G00139860
rosa_rugosa Rorug02G0112300 Rorug02G0112400
rosa_samantha Rh2AG161100 Rh2BG167900 Rh2CG168100 Rh2DG166800
rosa_wichuraiana Rw2G012640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 486
AciI CCGC 2 cut(s) 44, 89
AclWI GGATC 2 cut(s) 204, 379
AcoI YGGCCR 2 cut(s) 45, 90
AcsI RAATTY 3 cut(s) 517, 562, 587
AfiI CCNNNNNNNGG 2 cut(s) 43, 394
AflII CTTAAG 1 cut(s) 80
AgsI TTSAA 3 cut(s) 430, 560, 593
AluBI AGCT 4 cut(s) 79, 303, 411, 438
AluI AGCT 4 cut(s) 79, 303, 411, 438
Alw21I GWGCWC 2 cut(s) 492, 666
Alw26I GTCTC 3 cut(s) 104, 340, 464
AlwI GGATC 2 cut(s) 204, 379
Ama87I CYCGRG 1 cut(s) 343
AoxI GGCC 2 cut(s) 45, 90
ApeKI GCWGC 2 cut(s) 173, 481
ApoI RAATTY 3 cut(s) 517, 562, 587
AsuC2I CCSGG 1 cut(s) 375
AvaI CYCGRG 1 cut(s) 343
BbsI GAAGAC 2 cut(s) 35, 251
Bbv12I GWGCWC 2 cut(s) 492, 666
BbvI GCAGC 2 cut(s) 160, 468
BcnI CCSGG 1 cut(s) 375
BcoDI GTCTC 3 cut(s) 104, 340, 464
BfrI CTTAAG 1 cut(s) 80
BfuAI ACCTGC 1 cut(s) 486
BisI GCNGC 4 cut(s) 45, 90, 174, 482
BlsI GCNGC 4 cut(s) 46, 91, 175, 483
Bme1390I CCNGG 1 cut(s) 375
BmeT110I CYCGRG 1 cut(s) 343
BmiI GGNNCC 2 cut(s) 405, 622
BmrFI CCNGG 1 cut(s) 375
BmrI ACTGGG 1 cut(s) 32
BmsI GCATC 1 cut(s) 360
BmuI ACTGGG 1 cut(s) 32
BpiI GAAGAC 2 cut(s) 35, 251
BpmI CTGGAG 2 cut(s) 229, 386
BpuEI CTTGAG 1 cut(s) 642
BpuMI CCSGG 1 cut(s) 375
BsaJI CCNNGG 2 cut(s) 263, 535
BsaWI WCCGGW 1 cut(s) 495
Bsc4I CCNNNNNNNGG 2 cut(s) 43, 394
Bse1I ACTGG 3 cut(s) 38, 212, 610
BseDI CCNNGG 2 cut(s) 263, 535
BseGI GGATG 3 cut(s) 115, 476, 492
BseLI CCNNNNNNNGG 2 cut(s) 43, 394
BseMII CTCAG 1 cut(s) 627
BseNI ACTGG 3 cut(s) 38, 212, 610
BseRI GAGGAG 2 cut(s) 303, 452
BseXI GCAGC 2 cut(s) 160, 468
BseYI CCCAGC 1 cut(s) 438
Bsh1285I CGRYCG 1 cut(s) 500
BshFI GGCC 2 cut(s) 47, 92
BsiEI CGRYCG 1 cut(s) 500
BsiHKAI GWGCWC 2 cut(s) 492, 666
BsiHKCI CYCGRG 1 cut(s) 343
BsiSI CCGG 2 cut(s) 374, 496
BslFI GGGAC 1 cut(s) 154
BslI CCNNNNNNNGG 2 cut(s) 43, 394
BsmAI GTCTC 3 cut(s) 104, 340, 464
BsmFI GGGAC 1 cut(s) 154
BsnI GGCC 2 cut(s) 47, 92
BsoBI CYCGRG 1 cut(s) 343
Bsp1286I GDGCHC 2 cut(s) 492, 666
Bsp143I GATC 2 cut(s) 196, 384
Bsp19I CCATGG 1 cut(s) 535
BspACI CCGC 2 cut(s) 44, 89
BspANI GGCC 2 cut(s) 47, 92
BspCNI CTCAG 1 cut(s) 628
BspLI GGNNCC 2 cut(s) 405, 622
BspMI ACCTGC 1 cut(s) 486
BspPI GGATC 2 cut(s) 204, 379
BspQI GCTCTTC 1 cut(s) 648
BspTI CTTAAG 1 cut(s) 80
BsrI ACTGG 3 cut(s) 38, 212, 610
BssECI CCNNGG 2 cut(s) 263, 535
BssMI GATC 2 cut(s) 196, 384
BssT1I CCWWGG 2 cut(s) 263, 535
Bst4CI ACNGT 1 cut(s) 690
Bst6I CTCTTC 1 cut(s) 648
BstAFI CTTAAG 1 cut(s) 80
BstC8I GCNNGC 1 cut(s) 162
BstDEI CTNAG 2 cut(s) 235, 636
BstDSI CCRYGG 1 cut(s) 535
BstF5I GGATG 3 cut(s) 115, 476, 492
BstKTI GATC 2 cut(s) 199, 387
BstMAI GTCTC 3 cut(s) 104, 340, 464
BstMBI GATC 2 cut(s) 196, 384
BstMCI CGRYCG 1 cut(s) 500
BstMWI GCNNNNNNNGC 5 cut(s) 170, 317, 370, 487, 670
BstNSI RCATGY 1 cut(s) 599
BstSCI CCNGG 1 cut(s) 373
BstV1I GCAGC 2 cut(s) 160, 468
BstV2I GAAGAC 2 cut(s) 35, 251
BsuRI GGCC 2 cut(s) 47, 92
BtgI CCRYGG 1 cut(s) 535
BtsCI GGATG 3 cut(s) 115, 476, 492
BveI ACCTGC 1 cut(s) 486
Cac8I GCNNGC 1 cut(s) 162
CviAII CATG 2 cut(s) 536, 596
DdeI CTNAG 2 cut(s) 235, 636
DpnI GATC 2 cut(s) 198, 386
DpnII GATC 2 cut(s) 196, 384
EaeI YGGCCR 2 cut(s) 45, 90
Eam1104I CTCTTC 1 cut(s) 648
EarI CTCTTC 1 cut(s) 648
Eco130I CCWWGG 2 cut(s) 263, 535
Eco32I GATATC 1 cut(s) 70
Eco88I CYCGRG 1 cut(s) 343
EcoRV GATATC 1 cut(s) 70
EcoT14I CCWWGG 2 cut(s) 263, 535
ErhI CCWWGG 2 cut(s) 263, 535
FaeI CATG 2 cut(s) 539, 599
FaqI GGGAC 1 cut(s) 154
FatI CATG 2 cut(s) 535, 595
Fnu4HI GCNGC 4 cut(s) 45, 90, 174, 482
FokI GGATG 3 cut(s) 122, 479, 483
Fsp4HI GCNGC 4 cut(s) 45, 90, 174, 482
GluI GCNGC 4 cut(s) 45, 90, 174, 482
GsaI CCCAGC 1 cut(s) 442
GsuI CTGGAG 2 cut(s) 229, 386
HaeIII GGCC 2 cut(s) 47, 92
HapII CCGG 2 cut(s) 374, 496
Hin1II CATG 2 cut(s) 539, 599
HindIII AAGCTT 1 cut(s) 409
HinfI GANTC 2 cut(s) 97, 510
HpaII CCGG 2 cut(s) 374, 496
Hpy166II GTNNAC 1 cut(s) 13
Hpy188I TCNGA 3 cut(s) 67, 384, 515
Hpy188III TCNNGA 2 cut(s) 451, 659
Hpy8I GTNNAC 1 cut(s) 13
HpyAV CCTTC 2 cut(s) 398, 424
HpyCH4III ACNGT 1 cut(s) 690
HpyCH4V TGCA 2 cut(s) 484, 599
HpyF10VI GCNNNNNNNGC 5 cut(s) 170, 317, 370, 487, 670
HpyF3I CTNAG 2 cut(s) 235, 636
Hsp92II CATG 2 cut(s) 539, 599
Kzo9I GATC 2 cut(s) 196, 384
LguI GCTCTTC 1 cut(s) 648
LmnI GCTCC 1 cut(s) 409
Lsp1109I GCAGC 2 cut(s) 160, 468
LweI GCATC 1 cut(s) 360
MaeIII GTNAC 2 cut(s) 473, 631
MalI GATC 2 cut(s) 198, 386
MboI GATC 2 cut(s) 196, 384
MboII GAAGA 5 cut(s) 40, 251, 383, 406, 635
MhlI GDGCHC 2 cut(s) 492, 666
MluCI AATT 3 cut(s) 517, 562, 587
MlyI GAGTC 1 cut(s) 106
MmeI TCCRAC 1 cut(s) 277
MnlI CCTC 6 cut(s) 175, 281, 286, 473, 631, 666
MseI TTAA 2 cut(s) 81, 329
MspCI CTTAAG 1 cut(s) 80
MspI CCGG 2 cut(s) 374, 496
MspR9I CCNGG 1 cut(s) 375
MwoI GCNNNNNNNGC 5 cut(s) 170, 317, 370, 487, 670
NciI CCSGG 1 cut(s) 375
NcoI CCATGG 1 cut(s) 535
NdeII GATC 2 cut(s) 196, 384
NlaIII CATG 2 cut(s) 539, 599
NlaIV GGNNCC 2 cut(s) 405, 622
NmuCI GTSAC 1 cut(s) 631
NspI RCATGY 1 cut(s) 599
PciSI GCTCTTC 1 cut(s) 648
PfeI GAWTC 1 cut(s) 510
PkrI GCNGC 4 cut(s) 46, 91, 175, 483
PleI GAGTC 1 cut(s) 105
PpsI GAGTC 1 cut(s) 105
PspFI CCCAGC 1 cut(s) 438
PspN4I GGNNCC 2 cut(s) 405, 622
SapI GCTCTTC 1 cut(s) 648
SaqAI TTAA 2 cut(s) 81, 329
SatI GCNGC 4 cut(s) 45, 90, 174, 482
Sau3AI GATC 2 cut(s) 196, 384
SchI GAGTC 1 cut(s) 106
ScrFI CCNGG 1 cut(s) 375
SduI GDGCHC 2 cut(s) 492, 666
SetI ASST 8 cut(s) 81, 297, 305, 413, 428, 440, 480, 626
SfaNI GCATC 1 cut(s) 360
SmlI CTYRAG 2 cut(s) 80, 657
SmoI CTYRAG 2 cut(s) 80, 657
Sse9I AATT 3 cut(s) 517, 562, 587
SsiI CCGC 2 cut(s) 44, 89
StyD4I CCNGG 1 cut(s) 373
StyI CCWWGG 2 cut(s) 263, 535
TaaI ACNGT 1 cut(s) 690
TaqI TCGA 1 cut(s) 199
TasI AATT 3 cut(s) 517, 562, 587
TauI GCSGC 2 cut(s) 47, 92
TfiI GAWTC 1 cut(s) 510
Tru1I TTAA 2 cut(s) 81, 329
Tru9I TTAA 2 cut(s) 81, 329
TseFI GTSAC 1 cut(s) 631
TseI GCWGC 2 cut(s) 173, 481
Tsp45I GTSAC 1 cut(s) 631
TspDTI ATGAA 4 cut(s) 41, 74, 510, 635
Vha464I CTTAAG 1 cut(s) 80
XapI RAATTY 3 cut(s) 517, 562, 587
XceI RCATGY 1 cut(s) 599
XcmI CCANNNNNNNNNTGG 1 cut(s) 184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.