Rroxscaffold_2G00139860

Cold-regulated 413 plasma membrane protein 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0014958
Physical Location & Seq
Forward (+)
78153987 .. 78156671
2685 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_2G00139860.1

Sequence Viewer

Length: 612 bp
ATGGGGAGGGTGGACTATTTGGCTATGAAGACTGACCCAGTTGCGGCCACCGAGTTGATGAACTCTGATATCAATGAGCTTAAGATTGCGGCCAAGAGACTCGTTAGGGATGTTACTAGGCTCGGTGGTCTTGGTTTTGGGACTACTTTTCTCAAATGGCTTGCCTCCTTTGCTGCCATATATTTGTTGATATTGGATCGAACAAACTGGAGAACAAATATGCTGACTTCACTCTTAGTTCCTTATATTTTCTTTAGTCTTCCTTGGGGGCTGTTCCACTTACTGAGAGGAGAGGTTGGAAGCTGGATTGCTTTTGTTGCTGTTGTATTAAGGCTTTTCTTCCCAAGACATTTCCCGGATTGGCTGGAGATGCCGGGTTCTCTGATCCTTCTTCTGGTTGTGGCTCCAAGCTTCTTCGCAGAAACCTTGAAGGAGAGCTGGGTTGGCGTCCTGATATGTCTCCTCATTGGATGTTACCTGCTGCAAGAGCACATCCGGTCATCTGGTGGATTCAGAAATTCATTCACACAAAGCCATGGAATTTCAAACACTCTCGGTATTATCCTTCTCTTGGTCTACCCTGTATGGGCATTGGTCATCCACTTCATTTAA

Protein Analysis

203

Amino Acids

22.95

Weight (kDa)

9.03

Isoelectric Point (pI)

31.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WCOR413 PF05562 10 - 190 1.1e-88 Cold acclimation protein WCOR413
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014958)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50830
fragaria_vesca FvH4_1g14460
malus_domestica MD15G1273000.v1.1
prunus_persica Prupe.7G145800_v2.0.a1 Prupe.7G145800_v2.0.a1
pyrus_communis pycom15g23620
rosa_chinensis RchiOBHm_Chr2g0103221
rosa_laevigata RLG00000017215
rosa_roxburghii Rroxscaffold_2G00139860
rosa_rugosa Rorug02G0112300 Rorug02G0112400
rosa_samantha Rh2AG161100 Rh2BG167900 Rh2CG168100 Rh2DG166800
rosa_wichuraiana Rw2G012640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 486
AccI GTMKAC 1 cut(s) 576
AciI CCGC 2 cut(s) 44, 89
AclWI GGATC 2 cut(s) 204, 379
AcoI YGGCCR 2 cut(s) 45, 90
AcsI RAATTY 2 cut(s) 517, 540
AcyI GRCGYC 1 cut(s) 447
AfiI CCNNNNNNNGG 4 cut(s) 43, 394, 571, 586
AflII CTTAAG 1 cut(s) 80
AgsI TTSAA 2 cut(s) 430, 546
AluBI AGCT 4 cut(s) 79, 303, 411, 438
AluI AGCT 4 cut(s) 79, 303, 411, 438
Alw21I GWGCWC 1 cut(s) 492
Alw26I GTCTC 2 cut(s) 91, 464
AlwI GGATC 2 cut(s) 204, 379
AoxI GGCC 2 cut(s) 45, 90
ApeKI GCWGC 2 cut(s) 173, 481
ApoI RAATTY 2 cut(s) 517, 540
AsuC2I CCSGG 2 cut(s) 356, 375
BbsI GAAGAC 2 cut(s) 35, 251
Bbv12I GWGCWC 1 cut(s) 492
BbvI GCAGC 2 cut(s) 160, 468
BcnI CCSGG 2 cut(s) 356, 375
BcoDI GTCTC 2 cut(s) 91, 464
BfaI CTAG 1 cut(s) 117
BfrI CTTAAG 1 cut(s) 80
BfuAI ACCTGC 1 cut(s) 486
BisI GCNGC 4 cut(s) 45, 90, 174, 482
BlsI GCNGC 4 cut(s) 46, 91, 175, 483
Bme1390I CCNGG 2 cut(s) 356, 375
BmiI GGNNCC 1 cut(s) 405
BmrFI CCNGG 2 cut(s) 356, 375
BmrI ACTGGG 1 cut(s) 32
BmsI GCATC 1 cut(s) 360
BmuI ACTGGG 1 cut(s) 32
BpiI GAAGAC 2 cut(s) 35, 251
BpmI CTGGAG 2 cut(s) 229, 386
BpuMI CCSGG 2 cut(s) 356, 375
BsaHI GRCGYC 1 cut(s) 447
BsaJI CCNNGG 2 cut(s) 263, 535
BsaWI WCCGGW 1 cut(s) 495
Bsc4I CCNNNNNNNGG 4 cut(s) 43, 394, 571, 586
Bse1I ACTGG 2 cut(s) 38, 212
BseDI CCNNGG 2 cut(s) 263, 535
BseGI GGATG 4 cut(s) 115, 476, 492, 597
BseLI CCNNNNNNNGG 4 cut(s) 43, 394, 571, 586
BseMII CTCAG 1 cut(s) 275
BseNI ACTGG 2 cut(s) 38, 212
BseRI GAGGAG 2 cut(s) 303, 452
BseXI GCAGC 2 cut(s) 160, 468
BseYI CCCAGC 1 cut(s) 438
BshFI GGCC 2 cut(s) 47, 92
BsiHKAI GWGCWC 1 cut(s) 492
BsiSI CCGG 3 cut(s) 356, 374, 496
BslFI GGGAC 1 cut(s) 154
BslI CCNNNNNNNGG 4 cut(s) 43, 394, 571, 586
BsmAI GTCTC 2 cut(s) 91, 464
BsmFI GGGAC 1 cut(s) 154
BsnI GGCC 2 cut(s) 47, 92
Bsp1286I GDGCHC 1 cut(s) 492
Bsp143I GATC 2 cut(s) 196, 384
Bsp19I CCATGG 1 cut(s) 535
BspACI CCGC 2 cut(s) 44, 89
BspANI GGCC 2 cut(s) 47, 92
BspCNI CTCAG 1 cut(s) 276
BspLI GGNNCC 1 cut(s) 405
BspMI ACCTGC 1 cut(s) 486
BspPI GGATC 2 cut(s) 204, 379
BspTI CTTAAG 1 cut(s) 80
BsrI ACTGG 2 cut(s) 38, 212
BssECI CCNNGG 2 cut(s) 263, 535
BssMI GATC 2 cut(s) 196, 384
BssNI GRCGYC 1 cut(s) 447
BssT1I CCWWGG 2 cut(s) 263, 535
BstACI GRCGYC 1 cut(s) 447
BstAFI CTTAAG 1 cut(s) 80
BstC8I GCNNGC 1 cut(s) 162
BstDEI CTNAG 2 cut(s) 235, 284
BstDSI CCRYGG 1 cut(s) 535
BstF5I GGATG 4 cut(s) 115, 476, 492, 597
BstKTI GATC 2 cut(s) 199, 387
BstMAI GTCTC 2 cut(s) 91, 464
BstMBI GATC 2 cut(s) 196, 384
BstMWI GCNNNNNNNGC 5 cut(s) 170, 317, 370, 444, 487
BstSCI CCNGG 2 cut(s) 354, 373
BstV1I GCAGC 2 cut(s) 160, 468
BstV2I GAAGAC 2 cut(s) 35, 251
BsuRI GGCC 2 cut(s) 47, 92
BtgI CCRYGG 1 cut(s) 535
BtsCI GGATG 4 cut(s) 115, 476, 492, 597
BveI ACCTGC 1 cut(s) 486
Cac8I GCNNGC 1 cut(s) 162
CseI GACGC 1 cut(s) 436
CviAII CATG 1 cut(s) 536
DdeI CTNAG 2 cut(s) 235, 284
DpnI GATC 2 cut(s) 198, 386
DpnII GATC 2 cut(s) 196, 384
EaeI YGGCCR 2 cut(s) 45, 90
Eco130I CCWWGG 2 cut(s) 263, 535
Eco32I GATATC 1 cut(s) 70
EcoRV GATATC 1 cut(s) 70
EcoT14I CCWWGG 2 cut(s) 263, 535
ErhI CCWWGG 2 cut(s) 263, 535
FaeI CATG 1 cut(s) 539
FaiI YATR 8 cut(s) 26, 179, 181, 221, 246, 457, 537, 586
FaqI GGGAC 1 cut(s) 154
FatI CATG 1 cut(s) 535
FblI GTMKAC 1 cut(s) 576
Fnu4HI GCNGC 4 cut(s) 45, 90, 174, 482
FokI GGATG 4 cut(s) 122, 479, 483, 584
Fsp4HI GCNGC 4 cut(s) 45, 90, 174, 482
FspBI CTAG 1 cut(s) 117
GluI GCNGC 4 cut(s) 45, 90, 174, 482
GsaI CCCAGC 1 cut(s) 442
GsuI CTGGAG 2 cut(s) 229, 386
HaeIII GGCC 2 cut(s) 47, 92
HapII CCGG 3 cut(s) 356, 374, 496
HgaI GACGC 1 cut(s) 436
Hin1I GRCGYC 1 cut(s) 447
Hin1II CATG 1 cut(s) 539
HindIII AAGCTT 1 cut(s) 409
HinfI GANTC 2 cut(s) 99, 510
HpaII CCGG 3 cut(s) 356, 374, 496
Hpy166II GTNNAC 2 cut(s) 13, 577
Hpy188I TCNGA 3 cut(s) 67, 384, 515
Hpy188III TCNNGA 1 cut(s) 451
Hpy8I GTNNAC 2 cut(s) 13, 577
HpyAV CCTTC 3 cut(s) 398, 424, 575
HpyCH4V TGCA 1 cut(s) 484
HpyF10VI GCNNNNNNNGC 5 cut(s) 170, 317, 370, 444, 487
HpyF3I CTNAG 2 cut(s) 235, 284
Hsp92I GRCGYC 1 cut(s) 447
Hsp92II CATG 1 cut(s) 539
Kzo9I GATC 2 cut(s) 196, 384
LmnI GCTCC 1 cut(s) 409
Lsp1109I GCAGC 2 cut(s) 160, 468
LweI GCATC 1 cut(s) 360
MaeI CTAG 1 cut(s) 117
MaeIII GTNAC 2 cut(s) 112, 473
MalI GATC 2 cut(s) 198, 386
MboI GATC 2 cut(s) 196, 384
MboII GAAGA 5 cut(s) 40, 251, 331, 383, 406
MhlI GDGCHC 1 cut(s) 492
MluCI AATT 2 cut(s) 517, 540
MlyI GAGTC 1 cut(s) 93
MmeI TCCRAC 1 cut(s) 277
MnlI CCTC 4 cut(s) 175, 281, 286, 473
MseI TTAA 3 cut(s) 81, 329, 610
MspCI CTTAAG 1 cut(s) 80
MspI CCGG 3 cut(s) 356, 374, 496
MspR9I CCNGG 2 cut(s) 356, 375
MwoI GCNNNNNNNGC 5 cut(s) 170, 317, 370, 444, 487
NciI CCSGG 2 cut(s) 356, 375
NcoI CCATGG 1 cut(s) 535
NdeII GATC 2 cut(s) 196, 384
NlaIII CATG 1 cut(s) 539
NlaIV GGNNCC 1 cut(s) 405
PfeI GAWTC 1 cut(s) 510
PfoI TCCNGGA 1 cut(s) 354
PkrI GCNGC 4 cut(s) 46, 91, 175, 483
PleI GAGTC 1 cut(s) 93
PpsI GAGTC 1 cut(s) 93
PspFI CCCAGC 1 cut(s) 438
PspN4I GGNNCC 1 cut(s) 405
SaqAI TTAA 3 cut(s) 81, 329, 610
SatI GCNGC 4 cut(s) 45, 90, 174, 482
Sau3AI GATC 2 cut(s) 196, 384
SchI GAGTC 1 cut(s) 93
ScrFI CCNGG 2 cut(s) 356, 375
SduI GDGCHC 1 cut(s) 492
SetI ASST 7 cut(s) 81, 297, 305, 413, 428, 440, 480
SfaNI GCATC 1 cut(s) 360
SmlI CTYRAG 1 cut(s) 80
SmoI CTYRAG 1 cut(s) 80
Sse9I AATT 2 cut(s) 517, 540
SsiI CCGC 2 cut(s) 44, 89
SspMI CTAG 1 cut(s) 117
StyD4I CCNGG 2 cut(s) 354, 373
StyI CCWWGG 2 cut(s) 263, 535
TaqI TCGA 1 cut(s) 199
TasI AATT 2 cut(s) 517, 540
TauI GCSGC 2 cut(s) 47, 92
TfiI GAWTC 1 cut(s) 510
Tru1I TTAA 3 cut(s) 81, 329, 610
Tru9I TTAA 3 cut(s) 81, 329, 610
TseI GCWGC 2 cut(s) 173, 481
TspDTI ATGAA 4 cut(s) 41, 74, 510, 595
Vha464I CTTAAG 1 cut(s) 80
XapI RAATTY 2 cut(s) 517, 540
XmiI GTMKAC 1 cut(s) 576
XspI CTAG 1 cut(s) 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.