pycom15g24520

CTLH/CRA C-terminal to LisH motif domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008461
Physical Location & Seq
Reverse (-)
18712876 .. 18715663
2788 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1293 bp
ATGGACTCGACGCCAATTAACTGGGAAGCCCTAGACGCTCTCATCATCGATTTCGCCAAATCGGAGAAGCTAGTCGAAGACTCCTCCACTTTTACGACGTCGTCTTCCCCGCCCTCTTCCCCTCCGTCTTCCTCTTCCCCCTCCTCTATCTCCTCCTCGTCGTACCACTCGAGATTGATCATTCATCAGATCAGACGGTCGCTAGAGGCCGGTCACATTGACGCCGCCATCGGCCTCCTCCGCTCCCACGCCCCTTTCATTCTCGACGATCACAGGCTCCTCTTCCTCTTGCAGAAACAGAGGTTTATTGAGCTATTGAGGAGAGGGACCACAGAGGATCGTGACTCTGCAATCAATTGCTTGAGGAATGCTCTTGCTCCGTGTGCCCTCGACGCTTATCCGGAAGCTTATGGGGAATTCAAGCATGTACTGCTTGCTTTTATATATGACAAAGAAGACCAATCTTCTCCGGTAGCGACTGAGTGGTCTGAAAAGAGGAGGTTTGACATTGCCGGATTAGTTTCCACTGTCTTGAGAGCTTATTTACATGCTTATGATCCAGTCTTCTCGATGACCTTGAGATATTTGATAAGCATCCACAAAGGATTTTGTTTTCGTCAAGGAATTTCATCGCCTATTTCAGATCTTACACATAGATTACTTCTTGAGGAGCGTGACCCCCCTGCAACACCCCAGGAGAGCTTGTTTGAAGCACCTCCTTTTGATGAGGTGGACATACAAGCCCTTGCACATGCTGTAGAGCTCACGAGACAGGGAGCTGTAGATAGTTTGAGATTTGCTAAGGGTGATTTATTTCAGGCGTTTCAGAATGAATTATGCAGAATGAGATTTGATGTTGCCATGCTTGATGAGCTGGTTCGTGAGTATTGCGTTTACAGGGGCATTGTGGATTCTGGTTTTGCAACTTCTTCTGTGTCTGGGGTGCAAAGCCTTTCCAAATCTTTGAAAGTTGATCAACCGGTGATTGGGCATTCTCTGTCAAGAGACTGTTCTCATGAGGTCGATTATGGATCCAGCAAACATTCTGATGGTGAGATTTCTGTTAGTAATGATAATCTAGGTGGTTCCCCTGGAAAGAGTTCTGATGTGACGAGCATGCAAGGAATGGATATTGAAATGCGTTACGTTTGTGAGCCATCGAGCACTAATGAAGATTGTAGCACCAGTGGATCACATCAGCCTGATAAACTCAGAGTTTTACAAAGAAGACCCAGTGCACCTGCAGAAAGAAGTAAACGCAAGCGATGGAGGGGAAGACAAGATCGATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLTH

Protein Analysis

431

Amino Acids

47.95

Weight (kDa)

5.8

Isoelectric Point (pI)

64.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CTLH PF10607 62 - 181 1.3e-11 CTLH/CRA C-terminal to LisH motif domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1249
AasI GACNNNNNNGTC 1 cut(s) 484
AatII GACGTC 1 cut(s) 101
Acc36I ACCTGC 1 cut(s) 1249
AccBSI CCGCTC 1 cut(s) 243
AccIII TCCGGA 1 cut(s) 400
AciI CCGC 3 cut(s) 110, 225, 241
AclWI GGATC 5 cut(s) 345, 551, 1026, 1039, 1198
AcsI RAATTY 2 cut(s) 416, 624
AcyI GRCGYC 3 cut(s) 11, 98, 222
AfaI GTAC 2 cut(s) 164, 429
AfiI CCNNNNNNNGG 1 cut(s) 986
AgeI ACCGGT 1 cut(s) 979
AgsI TTSAA 4 cut(s) 421, 710, 967, 1136
AjnI CCWGG 2 cut(s) 693, 1090
AluBI AGCT 8 cut(s) 70, 313, 407, 539, 702, 763, 779, 874
AluI AGCT 8 cut(s) 70, 313, 407, 539, 702, 763, 779, 874
Alw21I GWGCWC 3 cut(s) 765, 1166, 1240
Alw26I GTCTC 2 cut(s) 763, 999
Alw44I GTGCAC 1 cut(s) 1236
AlwI GGATC 5 cut(s) 345, 551, 1026, 1039, 1198
Ama87I CYCGRG 1 cut(s) 169
Aor13HI TCCGGA 1 cut(s) 400
AoxI GGCC 2 cut(s) 207, 232
ApaLI GTGCAC 1 cut(s) 1236
ApoI RAATTY 2 cut(s) 416, 624
AsiGI ACCGGT 1 cut(s) 979
Asp700I GAANNNNTTC 1 cut(s) 1099
AspS9I GGNCC 1 cut(s) 327
AsuHPI GGTGA 3 cut(s) 818, 994, 1064
AvaI CYCGRG 1 cut(s) 169
AvaII GGWCC 1 cut(s) 327
BaeGI GKGCMC 2 cut(s) 388, 1240
BamHI GGATCC 1 cut(s) 1031
BanII GRGCYC 1 cut(s) 765
BauI CACGAG 1 cut(s) 766
BbsI GAAGAC 7 cut(s) 84, 96, 120, 462, 556, 1234, 1282
Bbv12I GWGCWC 3 cut(s) 765, 1166, 1240
BccI CCATC 4 cut(s) 236, 1043, 1165, 1260
BciT130I CCWGG 2 cut(s) 695, 1092
BclI TGATCA 2 cut(s) 177, 973
BcoDI GTCTC 2 cut(s) 763, 999
BfaI CTAG 4 cut(s) 32, 71, 203, 1079
BfmI CTRYAG 3 cut(s) 756, 780, 1242
BfuAI ACCTGC 1 cut(s) 1249
BglII AGATCT 1 cut(s) 643
BisI GCNGC 1 cut(s) 225
BlsI GCNGC 1 cut(s) 226
Bme1390I CCNGG 2 cut(s) 695, 1092
Bme18I GGWCC 1 cut(s) 327
BmeT110I CYCGRG 1 cut(s) 169
BmgT120I GGNCC 1 cut(s) 327
BmiI GGNNCC 4 cut(s) 278, 328, 1033, 1087
BmrFI CCNGG 2 cut(s) 695, 1092
BmrI ACTGGG 2 cut(s) 31, 1227
BmsI GCATC 1 cut(s) 603
BmuI ACTGGG 2 cut(s) 31, 1227
BpiI GAAGAC 7 cut(s) 84, 96, 120, 462, 556, 1234, 1282
BplI GAGNNNNNCTC 2 cut(s) 355, 387
Bpu10I CCTNAGC 1 cut(s) 801
BpuEI CTTGAG 4 cut(s) 382, 553, 598, 686
Bsa29I ATCGAT 2 cut(s) 48, 1285
BsaBI GATNNNNATC 1 cut(s) 593
BsaHI GRCGYC 3 cut(s) 11, 98, 222
BsaJI CCNNGG 2 cut(s) 693, 1090
BsaWI WCCGGW 3 cut(s) 400, 469, 979
BsaXI ACNNNNNCTCC 2 cut(s) 689, 719
Bsc4I CCNNNNNNNGG 1 cut(s) 986
Bse118I RCCGGY 2 cut(s) 209, 979
Bse1I ACTGG 4 cut(s) 26, 560, 1185, 1233
Bse3DI GCAATG 1 cut(s) 507
Bse8I GATNNNNATC 1 cut(s) 593
BseAI TCCGGA 1 cut(s) 400
BseBI CCWGG 2 cut(s) 695, 1092
BseCI ATCGAT 2 cut(s) 48, 1285
BseDI CCNNGG 2 cut(s) 693, 1090
BseGI GGATG 1 cut(s) 594
BseJI GATNNNNATC 1 cut(s) 593
BseLI CCNNNNNNNGG 1 cut(s) 986
BseMI GCAATG 1 cut(s) 507
BseMII CTCAG 2 cut(s) 471, 1225
BseNI ACTGG 4 cut(s) 26, 560, 1185, 1233
BseRI GAGGAG 9 cut(s) 73, 133, 142, 145, 227, 269, 334, 511, 683
BseSI GKGCMC 2 cut(s) 388, 1240
Bsh1285I CGRYCG 1 cut(s) 200
BshFI GGCC 2 cut(s) 209, 234
BshTI ACCGGT 1 cut(s) 979
BshVI ATCGAT 2 cut(s) 48, 1285
BsiEI CGRYCG 1 cut(s) 200
BsiHKAI GWGCWC 3 cut(s) 765, 1166, 1240
BsiHKCI CYCGRG 1 cut(s) 169
BsiSI CCGG 5 cut(s) 210, 401, 470, 513, 980
BslFI GGGAC 1 cut(s) 340
BslI CCNNNNNNNGG 1 cut(s) 986
BsmAI GTCTC 2 cut(s) 763, 999
BsmFI GGGAC 1 cut(s) 340
BsmI GAATGC 2 cut(s) 373, 991
BsnI GGCC 2 cut(s) 209, 234
BsoBI CYCGRG 1 cut(s) 169
Bsp1286I GDGCHC 4 cut(s) 388, 765, 1166, 1240
Bsp13I TCCGGA 1 cut(s) 400
BspACI CCGC 3 cut(s) 110, 225, 241
BspANI GGCC 2 cut(s) 209, 234
BspCNI CTCAG 2 cut(s) 472, 1224
BspDI ATCGAT 2 cut(s) 48, 1285
BspEI TCCGGA 1 cut(s) 400
BspHI TCATGA 1 cut(s) 1015
BspLI GGNNCC 4 cut(s) 278, 328, 1033, 1087
BspMAI CTGCAG 1 cut(s) 1246
BspMI ACCTGC 1 cut(s) 1249
BspPI GGATC 5 cut(s) 345, 551, 1026, 1039, 1198
BsrBI CCGCTC 1 cut(s) 243
BsrDI GCAATG 1 cut(s) 507
BsrFI RCCGGY 2 cut(s) 209, 979
BsrI ACTGG 4 cut(s) 26, 560, 1185, 1233
BssAI RCCGGY 2 cut(s) 209, 979
BssECI CCNNGG 2 cut(s) 693, 1090
BssNI GRCGYC 3 cut(s) 11, 98, 222
BssSI CACGAG 1 cut(s) 766
Bst2BI CACGAG 1 cut(s) 766
Bst2UI CCWGG 2 cut(s) 695, 1092
Bst4CI ACNGT 3 cut(s) 198, 529, 1010
Bst6I CTCTTC 3 cut(s) 121, 139, 287
BstACI GRCGYC 3 cut(s) 11, 98, 222
BstAPI GCANNNNNTGC 1 cut(s) 430
BstC8I GCNNGC 3 cut(s) 435, 1118, 1262
BstDEI CTNAG 3 cut(s) 480, 801, 1211
BstF5I GGATG 1 cut(s) 594
BstMAI GTCTC 2 cut(s) 763, 999
BstMCI CGRYCG 1 cut(s) 200
BstMWI GCNNNNNNNGC 6 cut(s) 35, 240, 383, 392, 430, 871
BstNI CCWGG 2 cut(s) 695, 1092
BstNSI RCATGY 4 cut(s) 428, 551, 755, 1120
BstSCI CCNGG 2 cut(s) 693, 1090
BstSFI CTRYAG 3 cut(s) 756, 780, 1242
BstSLI GKGCMC 2 cut(s) 388, 1240
BstV2I GAAGAC 7 cut(s) 84, 96, 120, 462, 556, 1234, 1282
BstX2I RGATCY 2 cut(s) 643, 1031
BstXI CCANNNNNNTGG 1 cut(s) 21
BstYI RGATCY 2 cut(s) 643, 1031
Bsu15I ATCGAT 2 cut(s) 48, 1285
BsuRI GGCC 2 cut(s) 209, 234
BsuTUI ATCGAT 2 cut(s) 48, 1285
BtgZI GCGATG 2 cut(s) 615, 1279
BtsCI GGATG 1 cut(s) 594
BtsIMutI CAGTG 3 cut(s) 525, 1192, 1240
BveI ACCTGC 1 cut(s) 1249
Cac8I GCNNGC 3 cut(s) 435, 1118, 1262
CciI TCATGA 1 cut(s) 1015
Cfr10I RCCGGY 2 cut(s) 209, 979
Cfr13I GGNCC 1 cut(s) 327
ClaI ATCGAT 2 cut(s) 48, 1285
CseI GACGC 4 cut(s) 19, 44, 230, 401
Csp6I GTAC 2 cut(s) 163, 428
CspAI ACCGGT 1 cut(s) 979
CviAII CATG 6 cut(s) 425, 548, 752, 862, 1016, 1117
CviQI GTAC 2 cut(s) 163, 428
DdeI CTNAG 3 cut(s) 480, 801, 1211
DrdI GACNNNNNNGTC 1 cut(s) 484
DseDI GACNNNNNNGTC 1 cut(s) 484
Eam1104I CTCTTC 3 cut(s) 121, 139, 287
EarI CTCTTC 3 cut(s) 121, 139, 287
Ecl136II GAGCTC 1 cut(s) 763
Eco24I GRGCYC 1 cut(s) 765
Eco47I GGWCC 1 cut(s) 327
Eco53kI GAGCTC 1 cut(s) 763
Eco88I CYCGRG 1 cut(s) 169
EcoICRI GAGCTC 1 cut(s) 763
EcoRI GAATTC 1 cut(s) 416
EcoRII CCWGG 2 cut(s) 693, 1090
EcoT38I GRGCYC 1 cut(s) 765
FaeI CATG 6 cut(s) 428, 551, 755, 865, 1019, 1120
FaqI GGGAC 1 cut(s) 340
FatI CATG 6 cut(s) 424, 547, 751, 861, 1015, 1116
FauI CCCGC 1 cut(s) 117
FbaI TGATCA 2 cut(s) 177, 973
Fnu4HI GCNGC 1 cut(s) 225
FokI GGATG 1 cut(s) 581
FriOI GRGCYC 1 cut(s) 765
Fsp4HI GCNGC 1 cut(s) 225
FspBI CTAG 4 cut(s) 32, 71, 203, 1079
GluI GCNGC 1 cut(s) 225
HaeIII GGCC 2 cut(s) 209, 234
HapII CCGG 5 cut(s) 210, 401, 470, 513, 980
HgaI GACGC 4 cut(s) 19, 44, 230, 401
Hin1I GRCGYC 3 cut(s) 11, 98, 222
Hin1II CATG 6 cut(s) 428, 551, 755, 865, 1019, 1120
HindIII AAGCTT 1 cut(s) 405
HinfI GANTC 4 cut(s) 5, 80, 344, 911
HpaII CCGG 5 cut(s) 210, 401, 470, 513, 980
HphI GGTGA 3 cut(s) 818, 994, 1064
Hpy166II GTNNAC 4 cut(s) 733, 895, 1238, 1256
Hpy188I TCNGA 9 cut(s) 64, 189, 194, 490, 643, 828, 1048, 1105, 1214
Hpy8I GTNNAC 4 cut(s) 733, 895, 1238, 1256
Hpy99I CGWCG 6 cut(s) 13, 100, 103, 163, 269, 395
HpyCH4III ACNGT 3 cut(s) 198, 529, 1010
HpyCH4IV ACGT 2 cut(s) 98, 1146
HpyF10VI GCNNNNNNNGC 6 cut(s) 35, 240, 383, 392, 430, 871
HpyF3I CTNAG 3 cut(s) 480, 801, 1211
HpySE526I ACGT 2 cut(s) 98, 1146
Hsp92I GRCGYC 3 cut(s) 11, 98, 222
Hsp92II CATG 6 cut(s) 428, 551, 755, 865, 1019, 1120
Kpn2I TCCGGA 1 cut(s) 400
Ksp22I TGATCA 2 cut(s) 177, 973
LmnI GCTCC 5 cut(s) 248, 282, 382, 670, 776
LweI GCATC 1 cut(s) 603
MaeI CTAG 4 cut(s) 32, 71, 203, 1079
MaeII ACGT 2 cut(s) 98, 1146
MaeIII GTNAC 5 cut(s) 212, 341, 674, 1108, 1142
MbiI CCGCTC 1 cut(s) 243
MfeI CAATTG 1 cut(s) 355
MflI RGATCY 2 cut(s) 643, 1031
MhlI GDGCHC 4 cut(s) 388, 765, 1166, 1240
MluCI AATT 5 cut(s) 15, 355, 416, 624, 833
MlyI GAGTC 2 cut(s) 74, 338
MroI TCCGGA 1 cut(s) 400
MroXI GAANNNNTTC 1 cut(s) 1099
MseI TTAA 1 cut(s) 18
MslI CAYNNNNRTG 2 cut(s) 552, 1047
MspI CCGG 5 cut(s) 210, 401, 470, 513, 980
MspR9I CCNGG 2 cut(s) 695, 1092
MunI CAATTG 1 cut(s) 355
Mva1269I GAATGC 2 cut(s) 373, 991
MvaI CCWGG 2 cut(s) 695, 1092
MwoI GCNNNNNNNGC 6 cut(s) 35, 240, 383, 392, 430, 871
NlaIII CATG 6 cut(s) 428, 551, 755, 865, 1019, 1120
NlaIV GGNNCC 4 cut(s) 278, 328, 1033, 1087
NmuCI GTSAC 4 cut(s) 212, 341, 674, 1108
NspI RCATGY 4 cut(s) 428, 551, 755, 1120
PaeI GCATGC 1 cut(s) 1120
PaeR7I CTCGAG 1 cut(s) 169
PagI TCATGA 1 cut(s) 1015
PaqCI CACCTGC 1 cut(s) 1249
PcsI WCGNNNNNNNCGW 1 cut(s) 167
PctI GAATGC 2 cut(s) 373, 991
PdmI GAANNNNTTC 1 cut(s) 1099
PfeI GAWTC 1 cut(s) 911
PflFI GACNNNGTC 1 cut(s) 100
PinAI ACCGGT 1 cut(s) 979
PkrI GCNGC 1 cut(s) 226
PleI GAGTC 2 cut(s) 74, 338
PpsI GAGTC 2 cut(s) 74, 338
Psp124BI GAGCTC 1 cut(s) 765
Psp6I CCWGG 2 cut(s) 693, 1090
PspGI CCWGG 2 cut(s) 693, 1090
PspN4I GGNNCC 4 cut(s) 278, 328, 1033, 1087
PspPI GGNCC 1 cut(s) 327
PstI CTGCAG 1 cut(s) 1246
PsuI RGATCY 2 cut(s) 643, 1031
PsyI GACNNNGTC 1 cut(s) 100
RsaI GTAC 2 cut(s) 164, 429
RsaNI GTAC 2 cut(s) 163, 428
RseI CAYNNNNRTG 2 cut(s) 552, 1047
SacI GAGCTC 1 cut(s) 765
SaqAI TTAA 1 cut(s) 18
SatI GCNGC 1 cut(s) 225
Sau96I GGNCC 1 cut(s) 327
SchI GAGTC 2 cut(s) 74, 338
ScrFI CCNGG 2 cut(s) 695, 1092
SduI GDGCHC 4 cut(s) 388, 765, 1166, 1240
SfaNI GCATC 1 cut(s) 603
SfcI CTRYAG 3 cut(s) 756, 780, 1242
Sfr274I CTCGAG 1 cut(s) 169
SinI GGWCC 1 cut(s) 327
SlaI CTCGAG 1 cut(s) 169
SmiMI CAYNNNNRTG 2 cut(s) 552, 1047
SmlI CTYRAG 5 cut(s) 169, 361, 532, 577, 665
SmoI CTYRAG 5 cut(s) 169, 361, 532, 577, 665
SphI GCATGC 1 cut(s) 1120
Sse9I AATT 5 cut(s) 15, 355, 416, 624, 833
SsiI CCGC 3 cut(s) 110, 225, 241
SspMI CTAG 4 cut(s) 32, 71, 203, 1079
SstI GAGCTC 1 cut(s) 765
StyD4I CCNGG 2 cut(s) 693, 1090
TaaI ACNGT 3 cut(s) 198, 529, 1010
TaiI ACGT 2 cut(s) 101, 1149
TasI AATT 5 cut(s) 15, 355, 416, 624, 833
TatI WGTACW 1 cut(s) 427
TauI GCSGC 1 cut(s) 227
TfiI GAWTC 1 cut(s) 911
Tru1I TTAA 1 cut(s) 18
Tru9I TTAA 1 cut(s) 18
TscAI CASTG 3 cut(s) 532, 1192, 1240
TseFI GTSAC 4 cut(s) 212, 341, 674, 1108
Tsp45I GTSAC 4 cut(s) 212, 341, 674, 1108
TspDTI ATGAA 5 cut(s) 173, 247, 618, 846, 1185
TspGWI ACGGA 2 cut(s) 114, 369
TspRI CASTG 3 cut(s) 532, 1192, 1240
Tth111I GACNNNGTC 1 cut(s) 100
VneI GTGCAC 1 cut(s) 1236
VpaK11BI GGWCC 1 cut(s) 327
XapI RAATTY 2 cut(s) 416, 624
XceI RCATGY 4 cut(s) 428, 551, 755, 1120
XhoI CTCGAG 1 cut(s) 169
XmnI GAANNNNTTC 1 cut(s) 1099
XspI CTAG 4 cut(s) 32, 71, 203, 1079
ZraI GACGTC 1 cut(s) 99
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.