pycom15g25610

DnaJ domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0003816
Physical Location & Seq
Forward (+)
20115804 .. 20119447
3644 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1593 bp
ATGGCTTCTCATGGCGGCAAAGACGACGTTCTGCTTCGCGGTCGTCCAATTCTTCTGCTATCACTTCAACAGAACCCAAGGCGCAATGTCTTATTTCTCTTCAATCGGAGTGGTGGAGCTCAGTGGTTCTGTAGCAGAACCGAGTCAGCTCGGACCGAGTTCGGCTCAGAAAACGCGTATGAGGTCCTAGGTGTTTCCGAAACCAGCTCATTTGCCGAAATCAAAGTGTCTTTCCACAAATTGGCGAAGCAAACTCACCCGGACCTCGCCGAGTCGAAGACCGATTCCGATGCTTCTCGCCGATTCGTGCAAATCCTCGCCGCCTATGAGATTCTTTCGGATTGCGAAAAGAGGGCCCATTACGACATGGACCTGTTATCTCAGAGAAAGCTTGTGCAGAAACATTCTGAACATGTCATGACATTCAGCATGTATAAGGGTCATCTAAGTACATATAAGAAGATGGAAGTTGTTGAATGGTTGAAGTGGTATAGACTAGCTATAAATGATGTTTTGTCAGAGAGGAGGGTGGTGGTTGGTACAGGTTATTTTGATGTTCTTGAGAGAGATTTCTATTCAGCCATACATGCGGCTTACTATGGCCCTCTGATAGAGTCTATGGAGCTTCTTCCGGACTGCTTCGAAGCTGAAGAGAGGTCTGTGTATGAAACTCCAGAGGTGCTGCATTTGGTTTCGGGGCGTGATCTTTTTGGGATGGTCCGTCTGGGCAATAAAATTCCAGAATTGTCATATTACAACAATATGAAATTGAATTATTCCACATCTGCGGGTTTGGGAATTTGTCATTCTGTTGAGGACTCTAGCATCAGCAAGAACTCTGGTGGCGTTGATGGTGTTGGAAACGCAAATACAGAAACTTGGGATATCAAAAATGACCCATCAGATGCATATAAAGATTTAGAATTGCATCTATGTGGAAGGGTGGTTGCTACAGCCACCAGAGTCCCTCCAAAAAACAATTGTGATGGGAAACAGAATCAAGAGTCTGATCGCATTCATGTTTTTCTCAGCTGTAATGAAAATCCTGTGCATACCAACAAAGGGTTTTCTCAAGATTCATTTTTAGGTGGTGCCATTGGATCAAGGATTCTGTTGGGTACAATAACAGGATTAGGGACTAGCTCAGAAGAAGGGTCTTGCGTTGTCTACAATAGCAGTGGTTCGAAAACCCATGTGATTATGAAGCATAGAACATTGCTGGTGAAACACTTGTATTGGTATGAATTGGGAGAAAATGTTTCTGTTTGTGAGTGCAGATGCAGTAGAGCTCGGTTGCCACCAAGCAAATTTTGGCTGTTTGAGCCTCGCTGTGGCATGCATGACATAGGCGGTTGGTATATTGAAACATTTGGTAGAGATAAGAAAGGCCGCATGTTGCCATCTCAACGATATTGGGATGGCTTTGATGCTGGAAAACAATTTGAAAAGAGACTCCATCCTGCGATGTATCTTCTTGCTCTTGCATATAGAACTTTAGATCTTGAAGATGCACAAAGAAGGAAAAATACATTCAGGGATGTCGTTGACAGACGACTTTTTAAAATTCTCAATTGGTGCAAAAGACTTATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

531

Amino Acids

60.35

Weight (kDa)

8.12

Isoelectric Point (pI)

44.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 58 - 122 3.8e-19 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1091
AccB7I CCANNNNNTGG 1 cut(s) 241
AccI GTMKAC 1 cut(s) 1167
AccII CGCG 2 cut(s) 39, 176
AccIII TCCGGA 1 cut(s) 631
AciI CCGC 7 cut(s) 15, 39, 321, 590, 788, 1350, 1390
AclWI GGATC 1 cut(s) 1108
AcsI RAATTY 4 cut(s) 735, 798, 1307, 1563
AcuI CTGAAG 1 cut(s) 669
AfaI GTAC 3 cut(s) 451, 541, 1120
AfiI CCNNNNNNNGG 3 cut(s) 241, 1062, 1331
AflIII ACRYGT 2 cut(s) 174, 412
AgsI TTSAA 8 cut(s) 68, 103, 476, 484, 772, 1364, 1445, 1505
Alw21I GWGCWC 2 cut(s) 121, 1291
Alw26I GTCTC 1 cut(s) 1444
AlwI GGATC 1 cut(s) 1108
Aor13HI TCCGGA 1 cut(s) 631
AoxI GGCC 3 cut(s) 354, 601, 1387
ApaI GGGCCC 1 cut(s) 358
ApeKI GCWGC 1 cut(s) 682
ApoI RAATTY 4 cut(s) 735, 798, 1307, 1563
AspA2I CCTAGG 1 cut(s) 187
AspLEI GCGC 1 cut(s) 84
AspS9I GGNCC 8 cut(s) 153, 184, 262, 354, 355, 370, 602, 718
AsuC2I CCSGG 1 cut(s) 260
AsuHPI GGTGA 2 cut(s) 248, 1234
AsuII TTCGAA 2 cut(s) 642, 1184
AvaII GGWCC 5 cut(s) 153, 184, 262, 370, 718
AvrII CCTAGG 1 cut(s) 187
BaeGI GKGCMC 1 cut(s) 358
BanI GGYRCC 1 cut(s) 1091
BanII GRGCYC 3 cut(s) 121, 358, 1291
BarI GAAGNNNNNNTAC 2 cut(s) 1510, 1542
BbsI GAAGAC 1 cut(s) 284
Bbv12I GWGCWC 2 cut(s) 121, 1291
BbvI GCAGC 1 cut(s) 669
BccI CCATC 8 cut(s) 457, 709, 845, 907, 980, 1408, 1412, 1464
BcgI CGANNNNNNTGC 2 cut(s) 272, 306
BcnI CCSGG 1 cut(s) 260
BcoDI GTCTC 1 cut(s) 1444
BfaI CTAG 4 cut(s) 188, 497, 822, 1140
BfmI CTRYAG 2 cut(s) 130, 951
BglII AGATCT 1 cut(s) 1498
BisI GCNGC 5 cut(s) 16, 321, 591, 683, 1390
BlnI CCTAGG 1 cut(s) 187
BlsI GCNGC 5 cut(s) 17, 322, 592, 684, 1391
Bme1390I CCNGG 1 cut(s) 260
Bme18I GGWCC 5 cut(s) 153, 184, 262, 370, 718
BmgT120I GGNCC 8 cut(s) 153, 184, 262, 354, 355, 370, 602, 718
BmiI GGNNCC 2 cut(s) 356, 1093
BmrFI CCNGG 1 cut(s) 260
BmsI GCATC 7 cut(s) 280, 834, 895, 937, 1268, 1417, 1498
BpiI GAAGAC 1 cut(s) 284
BplI GAGNNNNNCTC 2 cut(s) 149, 181
BpmI CTGGAG 1 cut(s) 657
Bpu14I TTCGAA 2 cut(s) 642, 1184
BpuEI CTTGAG 2 cut(s) 581, 1056
BpuMI CCSGG 1 cut(s) 260
BsaJI CCNNGG 2 cut(s) 77, 187
BsaWI WCCGGW 1 cut(s) 631
BsaXI ACNNNNNCTCC 2 cut(s) 1242, 1272
Bsc4I CCNNNNNNNGG 3 cut(s) 241, 1062, 1331
Bse3DI GCAATG 2 cut(s) 91, 1214
BseAI TCCGGA 1 cut(s) 631
BseDI CCNNGG 2 cut(s) 77, 187
BseGI GGATG 4 cut(s) 720, 1423, 1456, 1543
BseLI CCNNNNNNNGG 3 cut(s) 241, 1062, 1331
BseMI GCAATG 2 cut(s) 91, 1214
BseMII CTCAG 5 cut(s) 134, 180, 395, 1042, 1158
BseRI GAGGAG 1 cut(s) 538
BseSI GKGCMC 1 cut(s) 358
BseXI GCAGC 1 cut(s) 669
BsgI GTGCAG 2 cut(s) 416, 1294
Bsh1236I CGCG 2 cut(s) 39, 176
Bsh1285I CGRYCG 1 cut(s) 43
BshFI GGCC 3 cut(s) 356, 603, 1389
BshNI GGYRCC 1 cut(s) 1091
BsiEI CGRYCG 1 cut(s) 43
BsiHKAI GWGCWC 2 cut(s) 121, 1291
BsiSI CCGG 2 cut(s) 260, 632
BslFI GGGAC 2 cut(s) 950, 1150
BslI CCNNNNNNNGG 3 cut(s) 241, 1062, 1331
BsmAI GTCTC 1 cut(s) 1444
BsmFI GGGAC 2 cut(s) 950, 1150
BsmI GAATGC 1 cut(s) 1014
BsnI GGCC 3 cut(s) 356, 603, 1389
Bsp119I TTCGAA 2 cut(s) 642, 1184
Bsp120I GGGCCC 1 cut(s) 354
Bsp1286I GDGCHC 3 cut(s) 121, 358, 1291
Bsp13I TCCGGA 1 cut(s) 631
Bsp143I GATC 4 cut(s) 703, 1009, 1100, 1498
BspACI CCGC 7 cut(s) 15, 39, 321, 590, 788, 1350, 1390
BspANI GGCC 3 cut(s) 356, 603, 1389
BspCNI CTCAG 5 cut(s) 133, 179, 394, 1041, 1157
BspEI TCCGGA 1 cut(s) 631
BspFNI CGCG 2 cut(s) 39, 176
BspHI TCATGA 1 cut(s) 417
BspLI GGNNCC 2 cut(s) 356, 1093
BspPI GGATC 1 cut(s) 1108
BspT104I TTCGAA 2 cut(s) 642, 1184
BspT107I GGYRCC 1 cut(s) 1091
BsrDI GCAATG 2 cut(s) 91, 1214
BssECI CCNNGG 2 cut(s) 77, 187
BssMI GATC 4 cut(s) 703, 1009, 1100, 1498
BssT1I CCWWGG 2 cut(s) 77, 187
Bst6I CTCTTC 2 cut(s) 104, 645
BstBI TTCGAA 2 cut(s) 642, 1184
BstC8I GCNNGC 1 cut(s) 1337
BstDEI CTNAG 6 cut(s) 120, 166, 381, 446, 1028, 1144
BstF5I GGATG 4 cut(s) 720, 1423, 1456, 1543
BstFNI CGCG 2 cut(s) 39, 176
BstHHI GCGC 1 cut(s) 84
BstKTI GATC 4 cut(s) 706, 1012, 1103, 1501
BstMAI GTCTC 1 cut(s) 1444
BstMBI GATC 4 cut(s) 703, 1009, 1100, 1498
BstMCI CGRYCG 1 cut(s) 43
BstMWI GCNNNNNNNGC 2 cut(s) 587, 1321
BstNSI RCATGY 5 cut(s) 416, 433, 590, 1339, 1396
BstSCI CCNGG 1 cut(s) 258
BstSFI CTRYAG 2 cut(s) 130, 951
BstSLI GKGCMC 1 cut(s) 358
BstUI CGCG 2 cut(s) 39, 176
BstV1I GCAGC 1 cut(s) 669
BstV2I GAAGAC 1 cut(s) 284
BstX2I RGATCY 1 cut(s) 1498
BstYI RGATCY 1 cut(s) 1498
BsuRI GGCC 3 cut(s) 356, 603, 1389
BtgZI GCGATG 1 cut(s) 1478
BtsCI GGATG 4 cut(s) 720, 1423, 1456, 1543
BtsI GCAGTG 1 cut(s) 1183
BtsIMutI CAGTG 2 cut(s) 128, 1183
Cac8I GCNNGC 1 cut(s) 1337
CciI TCATGA 1 cut(s) 417
CfoI GCGC 1 cut(s) 84
Cfr13I GGNCC 8 cut(s) 153, 184, 262, 354, 355, 370, 602, 718
CpoI CGGWCCG 1 cut(s) 153
Csp6I GTAC 3 cut(s) 450, 540, 1119
CspCI CAANNNNNGTGG 4 cut(s) 91, 126, 1159, 1194
CspI CGGWCCG 1 cut(s) 153
CviQI GTAC 3 cut(s) 450, 540, 1119
DdeI CTNAG 6 cut(s) 120, 166, 381, 446, 1028, 1144
DpnI GATC 4 cut(s) 705, 1011, 1102, 1500
DpnII GATC 4 cut(s) 703, 1009, 1100, 1498
DraI TTTAAA 1 cut(s) 1561
Eam1104I CTCTTC 2 cut(s) 104, 645
EarI CTCTTC 2 cut(s) 104, 645
Ecl136II GAGCTC 2 cut(s) 119, 1289
Eco130I CCWWGG 2 cut(s) 77, 187
Eco24I GRGCYC 3 cut(s) 121, 358, 1291
Eco32I GATATC 1 cut(s) 886
Eco47I GGWCC 5 cut(s) 153, 184, 262, 370, 718
Eco53kI GAGCTC 2 cut(s) 119, 1289
Eco57I CTGAAG 1 cut(s) 669
EcoICRI GAGCTC 2 cut(s) 119, 1289
EcoO109I RGGNCCY 2 cut(s) 184, 354
EcoRV GATATC 1 cut(s) 886
EcoT14I CCWWGG 2 cut(s) 77, 187
EcoT22I ATGCAT 2 cut(s) 910, 1341
EcoT38I GRGCYC 3 cut(s) 121, 358, 1291
ErhI CCWWGG 2 cut(s) 77, 187
FaqI GGGAC 2 cut(s) 950, 1150
FauI CCCGC 1 cut(s) 781
FblI GTMKAC 1 cut(s) 1167
Fnu4HI GCNGC 5 cut(s) 16, 321, 591, 683, 1390
FokI GGATG 4 cut(s) 727, 1430, 1443, 1550
FriOI GRGCYC 3 cut(s) 121, 358, 1291
Fsp4HI GCNGC 5 cut(s) 16, 321, 591, 683, 1390
FspBI CTAG 4 cut(s) 188, 497, 822, 1140
GlaI GCGC 1 cut(s) 83
GluI GCNGC 5 cut(s) 16, 321, 591, 683, 1390
GsuI CTGGAG 1 cut(s) 657
HaeIII GGCC 3 cut(s) 356, 603, 1389
HapII CCGG 2 cut(s) 260, 632
HhaI GCGC 1 cut(s) 84
Hin6I GCGC 1 cut(s) 82
HinP1I GCGC 1 cut(s) 82
HincII GTYRAC 1 cut(s) 1546
HindII GTYRAC 1 cut(s) 1546
HindIII AAGCTT 1 cut(s) 389
HpaII CCGG 2 cut(s) 260, 632
HphI GGTGA 2 cut(s) 248, 1234
Hpy166II GTNNAC 2 cut(s) 1168, 1546
Hpy188III TCNNGA 8 cut(s) 418, 560, 632, 674, 740, 1001, 1073, 1502
Hpy8I GTNNAC 2 cut(s) 1168, 1546
Hpy99I CGWCG 1 cut(s) 29
HpyAV CCTTC 3 cut(s) 933, 1145, 1512
HpyCH4IV ACGT 1 cut(s) 27
HpyF10VI GCNNNNNNNGC 2 cut(s) 587, 1321
HpyF3I CTNAG 6 cut(s) 120, 166, 381, 446, 1028, 1144
HpySE526I ACGT 1 cut(s) 27
HspAI GCGC 1 cut(s) 82
Kpn2I TCCGGA 1 cut(s) 631
Kzo9I GATC 4 cut(s) 703, 1009, 1100, 1498
LmnI GCTCC 2 cut(s) 116, 622
Lsp1109I GCAGC 1 cut(s) 669
LweI GCATC 7 cut(s) 280, 834, 895, 937, 1268, 1417, 1498
MaeI CTAG 4 cut(s) 188, 497, 822, 1140
MaeII ACGT 1 cut(s) 27
MalI GATC 4 cut(s) 705, 1011, 1102, 1500
MboI GATC 4 cut(s) 703, 1009, 1100, 1498
MboII GAAGA 9 cut(s) 44, 91, 289, 472, 620, 662, 1160, 1463, 1517
MfeI CAATTG 2 cut(s) 979, 1570
MflI RGATCY 1 cut(s) 1498
MhlI GDGCHC 3 cut(s) 121, 358, 1291
MluI ACGCGT 1 cut(s) 174
MlyI GAGTC 7 cut(s) 152, 281, 623, 812, 972, 1013, 1446
MmeI TCCRAC 1 cut(s) 838
Mph1103I ATGCAT 2 cut(s) 910, 1341
MroI TCCGGA 1 cut(s) 631
MseI TTAA 2 cut(s) 1560, 1591
MslI CAYNNNNRTG 1 cut(s) 933
MspA1I CMGCKG 1 cut(s) 1032
MspI CCGG 2 cut(s) 260, 632
MspR9I CCNGG 1 cut(s) 260
MunI CAATTG 2 cut(s) 979, 1570
Mva1269I GAATGC 1 cut(s) 1014
MvnI CGCG 2 cut(s) 39, 176
MwoI GCNNNNNNNGC 2 cut(s) 587, 1321
NciI CCSGG 1 cut(s) 260
NdeII GATC 4 cut(s) 703, 1009, 1100, 1498
NlaIV GGNNCC 2 cut(s) 356, 1093
NmeAIII GCCGAG 1 cut(s) 295
NsiI ATGCAT 2 cut(s) 910, 1341
NspI RCATGY 5 cut(s) 416, 433, 590, 1339, 1396
NspV TTCGAA 2 cut(s) 642, 1184
PaeI GCATGC 1 cut(s) 1339
PagI TCATGA 1 cut(s) 417
PciI ACATGT 1 cut(s) 412
PctI GAATGC 1 cut(s) 1014
PfeI GAWTC 6 cut(s) 284, 303, 331, 997, 1076, 1108
PflMI CCANNNNNTGG 1 cut(s) 241
PkrI GCNGC 5 cut(s) 17, 322, 592, 684, 1391
PleI GAGTC 7 cut(s) 151, 280, 622, 812, 971, 1012, 1446
PpsI GAGTC 7 cut(s) 151, 280, 622, 812, 971, 1012, 1446
PpuMI RGGWCCY 1 cut(s) 184
PscI ACATGT 1 cut(s) 412
Psp124BI GAGCTC 2 cut(s) 121, 1291
Psp5II RGGWCCY 1 cut(s) 184
PspN4I GGNNCC 2 cut(s) 356, 1093
PspOMI GGGCCC 1 cut(s) 354
PspPI GGNCC 8 cut(s) 153, 184, 262, 354, 355, 370, 602, 718
PspPPI RGGWCCY 1 cut(s) 184
PsuI RGATCY 1 cut(s) 1498
PvuII CAGCTG 1 cut(s) 1032
RsaI GTAC 3 cut(s) 451, 541, 1120
RsaNI GTAC 3 cut(s) 450, 540, 1119
RseI CAYNNNNRTG 1 cut(s) 933
Rsr2I CGGWCCG 1 cut(s) 153
RsrII CGGWCCG 1 cut(s) 153
SacI GAGCTC 2 cut(s) 121, 1291
SaqAI TTAA 2 cut(s) 1560, 1591
SatI GCNGC 5 cut(s) 16, 321, 591, 683, 1390
Sau3AI GATC 4 cut(s) 703, 1009, 1100, 1498
Sau96I GGNCC 8 cut(s) 153, 184, 262, 354, 355, 370, 602, 718
SchI GAGTC 7 cut(s) 152, 281, 623, 812, 972, 1013, 1446
ScrFI CCNGG 1 cut(s) 260
SduI GDGCHC 3 cut(s) 121, 358, 1291
SfaNI GCATC 7 cut(s) 280, 834, 895, 937, 1268, 1417, 1498
SfcI CTRYAG 2 cut(s) 130, 951
SfuI TTCGAA 2 cut(s) 642, 1184
SinI GGWCC 5 cut(s) 153, 184, 262, 370, 718
SmiMI CAYNNNNRTG 1 cut(s) 933
SmlI CTYRAG 2 cut(s) 560, 1071
SmoI CTYRAG 2 cut(s) 560, 1071
SphI GCATGC 1 cut(s) 1339
SsiI CCGC 7 cut(s) 15, 39, 321, 590, 788, 1350, 1390
SspMI CTAG 4 cut(s) 188, 497, 822, 1140
SstI GAGCTC 2 cut(s) 121, 1291
StyD4I CCNGG 1 cut(s) 258
StyI CCWWGG 2 cut(s) 77, 187
TaiI ACGT 1 cut(s) 30
TaqI TCGA 3 cut(s) 275, 642, 1184
TaqII GACCGA 2 cut(s) 170, 296
TatI WGTACW 1 cut(s) 449
TauI GCSGC 4 cut(s) 18, 323, 593, 1392
TfiI GAWTC 6 cut(s) 284, 303, 331, 997, 1076, 1108
Tru1I TTAA 2 cut(s) 1560, 1591
Tru9I TTAA 2 cut(s) 1560, 1591
TscAI CASTG 2 cut(s) 128, 1183
TseI GCWGC 1 cut(s) 682
TspDTI ATGAA 7 cut(s) 681, 779, 1007, 1053, 1068, 1217, 1257
TspGWI ACGGA 1 cut(s) 710
TspRI CASTG 2 cut(s) 128, 1183
Van91I CCANNNNNTGG 1 cut(s) 241
VpaK11BI GGWCC 5 cut(s) 153, 184, 262, 370, 718
XapI RAATTY 4 cut(s) 735, 798, 1307, 1563
XceI RCATGY 5 cut(s) 416, 433, 590, 1339, 1396
XcmI CCANNNNNNNNNTGG 1 cut(s) 1308
XmaJI CCTAGG 1 cut(s) 187
XmiI GTMKAC 1 cut(s) 1167
XspI CTAG 4 cut(s) 188, 497, 822, 1140
Zsp2I ATGCAT 2 cut(s) 910, 1341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.