pycom15g25700

expressed protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008653
Physical Location & Seq
Reverse (-)
20220375 .. 20221270
896 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 714 bp
ATGGAGAAGCTTCTCAAGCCATATGATAAGGAGTACATGAGGATGGCCATTTTAAAGCACGAAGAAACTTTCAAAGAGCAGGTATATGAACTTCATAGGCTATATCATTTGCAGAAGATATTGATGAAAAGCTCCATTGGAAAAAGAAGGCCCAATGGACAGAAACAAGAGAGTTGGGATTTAAATTATCATGACAACCAAGAGGCACAATTTAACCCACAAAGGAAACTAGATTTAGAACAGCCTGCAGCAGCAGCAGCAGCAGGAGAGTACGTTGCAGACACAGAAGGGGATGATGGGGTGCTAGAGATCATAGATGAGAGTGAGATTGAGTTGACTCTAGGCCCTACCAGATACAACAACAACAGCAACAACAGCAACAAAAGCAGCAGCAGCAACAGAAGGAAGAGAGGAGGAGAGGCAGCGGCACTAACACTATCAGATTCAGCAGGTCCAAGTAGTTTCTCATCTTCTTGTTCTACTGGATCAAGCCATACAATAAACAGGAGAAGTAGTTTCTCCAGGAGGACCAATCAAAGTACTATACGTGGGCAGTTGCTGCCTGCTGACATGGCATCAGGAGGGTACAGAAGAAGTGGAGGGGGTAAAATCAGCAGCAGCAATGTTAATGGTGGAGAAGAACAGTTGAGACAGGATGAGAGGCTAAAACTGCCTCCTTGGCTTTTCCAAGTTTTGAGTTTGAACATGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

238

Amino Acids

26.53

Weight (kDa)

8.56

Isoelectric Point (pI)

64.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 70, 440
AciI CCGC 1 cut(s) 425
AclWI GGATC 1 cut(s) 493
AcoI YGGCCR 1 cut(s) 45
AfaI GTAC 4 cut(s) 35, 272, 541, 587
AgsI TTSAA 2 cut(s) 73, 703
AjnI CCWGG 1 cut(s) 521
AluBI AGCT 2 cut(s) 10, 132
AluI AGCT 2 cut(s) 10, 132
Alw26I GTCTC 1 cut(s) 643
AlwI GGATC 1 cut(s) 493
AlwNI CAGNNNCTG 1 cut(s) 559
AoxI GGCC 3 cut(s) 45, 149, 343
AspS9I GGNCC 4 cut(s) 150, 344, 452, 528
AvaII GGWCC 2 cut(s) 452, 528
BalI TGGCCA 1 cut(s) 47
BarI GAAGNNNNNNTAC 2 cut(s) 75, 107
BccI CCATC 2 cut(s) 37, 290
BciT130I CCWGG 1 cut(s) 523
BcoDI GTCTC 1 cut(s) 643
BfaI CTAG 3 cut(s) 230, 305, 341
BfmI CTRYAG 1 cut(s) 246
BfuAI ACCTGC 2 cut(s) 70, 440
BglI GCCNNNNNGGC 1 cut(s) 679
BmcAI AGTACT 1 cut(s) 541
Bme1390I CCNGG 1 cut(s) 523
Bme18I GGWCC 2 cut(s) 452, 528
BmgT120I GGNCC 4 cut(s) 150, 344, 452, 528
BmrFI CCNGG 1 cut(s) 523
BmsI GCATC 1 cut(s) 584
BpmI CTGGAG 1 cut(s) 505
BsaAI YACGTR 1 cut(s) 548
BsaJI CCNNGG 1 cut(s) 677
BsaXI ACNNNNNCTCC 4 cut(s) 258, 288, 499, 529
Bse1I ACTGG 1 cut(s) 487
Bse3DI GCAATG 1 cut(s) 628
BseBI CCWGG 1 cut(s) 523
BseDI CCNNGG 1 cut(s) 677
BseGI GGATG 3 cut(s) 48, 298, 661
BseMI GCAATG 1 cut(s) 628
BseNI ACTGG 1 cut(s) 487
BseRI GAGGAG 2 cut(s) 426, 429
BshFI GGCC 3 cut(s) 47, 151, 345
BsmAI GTCTC 1 cut(s) 643
BsnI GGCC 3 cut(s) 47, 151, 345
Bsp143I GATC 2 cut(s) 309, 485
BspACI CCGC 1 cut(s) 425
BspANI GGCC 3 cut(s) 47, 151, 345
BspHI TCATGA 1 cut(s) 190
BspMAI CTGCAG 1 cut(s) 250
BspMI ACCTGC 2 cut(s) 70, 440
BspPI GGATC 1 cut(s) 493
BsrDI GCAATG 1 cut(s) 628
BsrI ACTGG 1 cut(s) 487
BssECI CCNNGG 1 cut(s) 677
BssMI GATC 2 cut(s) 309, 485
BssT1I CCWWGG 1 cut(s) 677
Bst2UI CCWGG 1 cut(s) 523
Bst4CI ACNGT 1 cut(s) 645
Bst6I CTCTTC 1 cut(s) 401
BstAPI GCANNNNNTGC 1 cut(s) 559
BstBAI YACGTR 1 cut(s) 548
BstC8I GCNNGC 2 cut(s) 246, 564
BstF5I GGATG 3 cut(s) 48, 298, 661
BstKTI GATC 2 cut(s) 312, 488
BstMAI GTCTC 1 cut(s) 643
BstMBI GATC 2 cut(s) 309, 485
BstNI CCWGG 1 cut(s) 523
BstSCI CCNGG 1 cut(s) 521
BstSFI CTRYAG 1 cut(s) 246
BsuRI GGCC 3 cut(s) 47, 151, 345
BtsCI GGATG 3 cut(s) 48, 298, 661
BveI ACCTGC 2 cut(s) 70, 440
Cac8I GCNNGC 2 cut(s) 246, 564
CaiI CAGNNNCTG 1 cut(s) 559
CciI TCATGA 1 cut(s) 190
Cfr13I GGNCC 4 cut(s) 150, 344, 452, 528
Csp6I GTAC 4 cut(s) 34, 271, 540, 586
CviAII CATG 4 cut(s) 37, 191, 571, 706
CviQI GTAC 4 cut(s) 34, 271, 540, 586
DpnI GATC 2 cut(s) 311, 487
DpnII GATC 2 cut(s) 309, 485
DraI TTTAAA 2 cut(s) 54, 183
EaeI YGGCCR 1 cut(s) 45
Eam1104I CTCTTC 1 cut(s) 401
EarI CTCTTC 1 cut(s) 401
Eco130I CCWWGG 1 cut(s) 677
Eco47I GGWCC 2 cut(s) 452, 528
EcoO109I RGGNCCY 1 cut(s) 344
EcoRII CCWGG 1 cut(s) 521
EcoT14I CCWWGG 1 cut(s) 677
ErhI CCWWGG 1 cut(s) 677
FaeI CATG 4 cut(s) 40, 194, 574, 709
FatI CATG 4 cut(s) 36, 190, 570, 705
FauNDI CATATG 1 cut(s) 22
FokI GGATG 3 cut(s) 55, 305, 668
FspBI CTAG 3 cut(s) 230, 305, 341
GsuI CTGGAG 1 cut(s) 505
HaeIII GGCC 3 cut(s) 47, 151, 345
Hin1II CATG 4 cut(s) 40, 194, 574, 709
HincII GTYRAC 1 cut(s) 336
HindII GTYRAC 1 cut(s) 336
HindIII AAGCTT 1 cut(s) 8
HinfI GANTC 2 cut(s) 337, 443
Hpy166II GTNNAC 1 cut(s) 336
Hpy188I TCNGA 1 cut(s) 442
Hpy188III TCNNGA 2 cut(s) 191, 579
Hpy8I GTNNAC 1 cut(s) 336
HpyAV CCTTC 3 cut(s) 141, 281, 396
HpyCH4III ACNGT 1 cut(s) 645
HpyCH4IV ACGT 2 cut(s) 273, 547
HpyCH4V TGCA 3 cut(s) 112, 248, 278
HpySE526I ACGT 2 cut(s) 273, 547
Hsp92II CATG 4 cut(s) 40, 194, 574, 709
Kzo9I GATC 2 cut(s) 309, 485
LmnI GCTCC 1 cut(s) 137
LweI GCATC 1 cut(s) 584
MaeI CTAG 3 cut(s) 230, 305, 341
MaeII ACGT 2 cut(s) 273, 547
MalI GATC 2 cut(s) 311, 487
MboI GATC 2 cut(s) 309, 485
MboII GAAGA 6 cut(s) 74, 127, 418, 462, 603, 650
MlsI TGGCCA 1 cut(s) 47
MluCI AATT 2 cut(s) 184, 209
MluNI TGGCCA 1 cut(s) 47
MlyI GAGTC 1 cut(s) 331
Mox20I TGGCCA 1 cut(s) 47
MscI TGGCCA 1 cut(s) 47
MseI TTAA 4 cut(s) 53, 182, 213, 627
MslI CAYNNNNRTG 1 cut(s) 41
Msp20I TGGCCA 1 cut(s) 47
MspA1I CMGCKG 1 cut(s) 425
MspR9I CCNGG 1 cut(s) 523
MvaI CCWGG 1 cut(s) 523
NdeI CATATG 1 cut(s) 22
NdeII GATC 2 cut(s) 309, 485
NlaIII CATG 4 cut(s) 40, 194, 574, 709
PagI TCATGA 1 cut(s) 190
PfeI GAWTC 1 cut(s) 443
PfoI TCCNGGA 1 cut(s) 521
PleI GAGTC 1 cut(s) 331
PpsI GAGTC 1 cut(s) 331
Ppu21I YACGTR 1 cut(s) 548
Psp6I CCWGG 1 cut(s) 521
PspGI CCWGG 1 cut(s) 521
PspPI GGNCC 4 cut(s) 150, 344, 452, 528
PstI CTGCAG 1 cut(s) 250
PstNI CAGNNNCTG 1 cut(s) 559
RsaI GTAC 4 cut(s) 35, 272, 541, 587
RsaNI GTAC 4 cut(s) 34, 271, 540, 586
RseI CAYNNNNRTG 1 cut(s) 41
SaqAI TTAA 4 cut(s) 53, 182, 213, 627
Sau3AI GATC 2 cut(s) 309, 485
Sau96I GGNCC 4 cut(s) 150, 344, 452, 528
ScaI AGTACT 1 cut(s) 541
SchI GAGTC 1 cut(s) 331
ScrFI CCNGG 1 cut(s) 523
SetI ASST 6 cut(s) 12, 84, 134, 276, 454, 550
SfaNI GCATC 1 cut(s) 584
SfcI CTRYAG 1 cut(s) 246
SinI GGWCC 2 cut(s) 452, 528
SmiI ATTTAAAT 1 cut(s) 183
SmiMI CAYNNNNRTG 1 cut(s) 41
SmlI CTYRAG 1 cut(s) 14
SmoI CTYRAG 1 cut(s) 14
Sse9I AATT 2 cut(s) 184, 209
SsiI CCGC 1 cut(s) 425
SspMI CTAG 3 cut(s) 230, 305, 341
StyD4I CCNGG 1 cut(s) 521
StyI CCWWGG 1 cut(s) 677
SwaI ATTTAAAT 1 cut(s) 183
TaaI ACNGT 1 cut(s) 645
TaiI ACGT 2 cut(s) 276, 550
TasI AATT 2 cut(s) 184, 209
TatI WGTACW 2 cut(s) 33, 539
TauI GCSGC 1 cut(s) 428
TfiI GAWTC 1 cut(s) 443
Tru1I TTAA 4 cut(s) 53, 182, 213, 627
Tru9I TTAA 4 cut(s) 53, 182, 213, 627
TspDTI ATGAA 3 cut(s) 83, 102, 140
VpaK11BI GGWCC 2 cut(s) 452, 528
XspI CTAG 3 cut(s) 230, 305, 341
ZrmI AGTACT 1 cut(s) 541
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.