pycom15g29110

aarF domain-containing protein kinase At1g79600

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0007840
Physical Location & Seq
Reverse (-)
25751248 .. 25757903
6656 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g29110.1

Sequence Viewer

Length: 1380 bp
ATGGCTACTTGTTCTCTACCTCTACTGGAACTGACCTTTCTCTCACCACAAACCACTCCTAAACGCCGCCTTTCTACCTCTGGAGTTTCTCTCTCTAGATTCTCTCTTATTGGATACCCTAGAGGCGGTGTTCGATCCCGAATCAGAGCGACTAAAGAGGATGGCCTTGTAGTTGAAAAGACGATCAACAAAGTCAATGGTGTCGAGTTGGGTGGTAATGGCGCTGCTGCTAGTACTAGTGGTAGCAATTACGGGTCCAATGGCTCGGTCAACAGCTACTCGAATGGCAGTGCTAATGGGACGAGTAACGGGAGCTTGGTGAAGTTCGTGAATGGCAATGGAGTGGCAGCGGAAGTGGTGGAAGGAGTTCAAGTGTTGGAGACGAAGGAGGAAGGAAGGAGGAAGAATATTGAGGAAATTGGAAAGGAAGATGCGTGGTTTAAGCAAAGCGGGCTACCAAAAGTCGAGGTCTCTGTTGCACCTGGTGGCCGTTGGAGTAGATTTAAAACTTACTCGACAATCCAGAGGACCTTGGAAATATGGGGATTTGTTCTAAAATTCATTATAAAGGCTTGGTTAGACAATCAAAAATTTACTTATCAAGGGGGAATGACAGAGGAGAAGAAAACCTTGAGGCGTAAGGCACTTGCCAAGTGGCTTAAGGAGAATATTTTGAGATTAGGTCCAACATTCATTAAAATTGGCCAGCAGTTCTCCACAAGGGTGGACATTCTTGCTCAAGAATATGATGATCAATTGTCAGAACTTCAGGATCAAGTTCCTCCTTTCCCTTCAGATACAGCTATTTCTATTGTTGAAGAAGAGCTTGGTGCGCCTGTGGGAGATATCTTTGATCGGTTTGATTATGAACCAATAGCTGCTGCTAGTCTTGGTCAGGTTCACCGTGCAAGATTGAAAGGACAGGAAGTTGTTGTTAAAGTACAGAGGCCTGGACTTAAGGATCTTTTCAATATTGATCTTAAGAACCTGAGGGTAATTGCAGAATATCTTCAAAAAATTGACCCGAAGTCAGATGGTGCGAAGAGGGATTGGGTTGCTATCTATGATGAGTGTGCAAATGTCTTATATGAGGAGATTGATTATACCAAGGAAGCTGCTAATGCTGAACTATTTGCAAGTAACTTCAAAAACATGGATTATGTCAAAGTCCCAACCATAATATGGGAATACACTACACCACAGGTTCTGACAATGGAGTATGTCCCGGGGATCAAGATTAACAAGATACAAGCTATAGATCAATTGGGCCTTGATCGGCAAAGGCTGGGCCGGTATGCTGTTGAATCGTACTTGGAACAAATTCTGTCTCATGGATTTTTTCATGCTCGATCCTCATCCCGGAAATATTGCTGTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC1

Protein Analysis

460

Amino Acids

51.18

Weight (kDa)

8.74

Isoelectric Point (pI)

41.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC1 PF03109 256 - 449 1.3e-66 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 566
AccB7I CCANNNNNTGG 1 cut(s) 1182
AciI CCGC 4 cut(s) 67, 126, 350, 450
AclWI GGATC 5 cut(s) 129, 780, 969, 1238, 1344
AcoI YGGCCR 2 cut(s) 487, 703
AcsI RAATTY 3 cut(s) 557, 590, 1320
AcuI CTGAAG 2 cut(s) 752, 777
AdeI CACNNNGTG 1 cut(s) 485
AfaI GTAC 3 cut(s) 235, 942, 1310
AfiI CCNNNNNNNGG 3 cut(s) 125, 1182, 1359
AflII CTTAAG 3 cut(s) 659, 956, 980
AgsI TTSAA 8 cut(s) 176, 371, 818, 916, 970, 1013, 1147, 1304
AhdI GACNNNNNGTC 1 cut(s) 1027
AhlI ACTAGT 1 cut(s) 236
AjnI CCWGG 2 cut(s) 481, 949
AjuI GAANNNNNNNTTGG 2 cut(s) 810, 842
AleI CACNNNNGTG 1 cut(s) 722
AluBI AGCT 7 cut(s) 276, 315, 803, 826, 878, 1115, 1253
AluI AGCT 7 cut(s) 276, 315, 803, 826, 878, 1115, 1253
Alw26I GTCTC 3 cut(s) 374, 475, 1332
AlwI GGATC 5 cut(s) 129, 780, 969, 1238, 1344
AlwNI CAGNNNCTG 1 cut(s) 1207
Ama87I CYCGRG 1 cut(s) 1225
AoxI GGCC 6 cut(s) 163, 487, 703, 947, 1267, 1288
ApeKI GCWGC 6 cut(s) 224, 227, 347, 878, 881, 1115
ApoI RAATTY 3 cut(s) 557, 590, 1320
Asp700I GAANNNNTTC 3 cut(s) 366, 1008, 1320
AspLEI GCGC 2 cut(s) 224, 835
AspS9I GGNCC 5 cut(s) 255, 528, 683, 1267, 1288
AsuC2I CCSGG 3 cut(s) 1226, 1227, 1360
AsuHPI GGTGA 3 cut(s) 36, 331, 893
AvaI CYCGRG 1 cut(s) 1225
AvaII GGWCC 3 cut(s) 255, 528, 683
AxyI CCTNAGG 1 cut(s) 989
BalI TGGCCA 1 cut(s) 705
BbvI GCAGC 6 cut(s) 211, 214, 359, 865, 868, 1102
BccI CCATC 2 cut(s) 155, 1028
BceAI ACGGC 1 cut(s) 474
BciT130I CCWGG 2 cut(s) 483, 951
BciVI GTATCC 1 cut(s) 107
BclI TGATCA 1 cut(s) 751
BcnI CCSGG 3 cut(s) 1226, 1227, 1360
BcoDI GTCTC 3 cut(s) 374, 475, 1332
BcuI ACTAGT 1 cut(s) 236
BfaI CTAG 5 cut(s) 96, 120, 231, 237, 885
BfmI CTRYAG 1 cut(s) 1254
BfoI RGCGCY 1 cut(s) 225
BfrI CTTAAG 3 cut(s) 659, 956, 980
BfuI GTATCC 1 cut(s) 107
BisI GCNGC 7 cut(s) 67, 225, 228, 348, 879, 882, 1116
BlsI GCNGC 7 cut(s) 68, 226, 229, 349, 880, 883, 1117
BmcAI AGTACT 1 cut(s) 235
Bme1390I CCNGG 5 cut(s) 483, 951, 1226, 1227, 1360
Bme18I GGWCC 3 cut(s) 255, 528, 683
BmeRI GACNNNNNGTC 1 cut(s) 1027
BmeT110I CYCGRG 1 cut(s) 1225
BmgT120I GGNCC 5 cut(s) 255, 528, 683, 1267, 1288
BmiI GGNNCC 1 cut(s) 256
BmrFI CCNGG 5 cut(s) 483, 951, 1226, 1227, 1360
BmsI GCATC 1 cut(s) 421
BplI GAGNNNNNCTC 2 cut(s) 75, 107
BpmI CTGGAG 1 cut(s) 102
BpuEI CTTGAG 2 cut(s) 652, 723
BpuMI CCSGG 3 cut(s) 1226, 1227, 1360
BsaBI GATNNNNATC 1 cut(s) 1354
BsaI GGTCTC 1 cut(s) 475
BsaJI CCNNGG 4 cut(s) 531, 1107, 1225, 1226
BsaXI ACNNNNNCTCC 2 cut(s) 357, 387
Bsc4I CCNNNNNNNGG 3 cut(s) 125, 1182, 1359
Bse118I RCCGGY 1 cut(s) 1290
Bse1I ACTGG 1 cut(s) 30
Bse21I CCTNAGG 1 cut(s) 989
Bse3DI GCAATG 1 cut(s) 343
Bse8I GATNNNNATC 1 cut(s) 1354
BseBI CCWGG 2 cut(s) 483, 951
BseDI CCNNGG 4 cut(s) 531, 1107, 1225, 1226
BseGI GGATG 2 cut(s) 166, 1355
BseJI GATNNNNATC 1 cut(s) 1354
BseLI CCNNNNNNNGG 3 cut(s) 125, 1182, 1359
BseMI GCAATG 1 cut(s) 343
BseMII CTCAG 1 cut(s) 980
BseNI ACTGG 1 cut(s) 30
BseRI GAGGAG 2 cut(s) 632, 1106
BseXI GCAGC 6 cut(s) 211, 214, 359, 865, 868, 1102
BseYI CCCAGC 1 cut(s) 1285
BshFI GGCC 6 cut(s) 165, 489, 705, 949, 1269, 1290
BsiHKCI CYCGRG 1 cut(s) 1225
BsiSI CCGG 3 cut(s) 1226, 1291, 1360
BslFI GGGAC 3 cut(s) 313, 1154, 1208
BslI CCNNNNNNNGG 3 cut(s) 125, 1182, 1359
BsmAI GTCTC 3 cut(s) 374, 475, 1332
BsmBI CGTCTC 1 cut(s) 374
BsmFI GGGAC 3 cut(s) 313, 1154, 1208
BsnI GGCC 6 cut(s) 165, 489, 705, 949, 1269, 1290
Bso31I GGTCTC 1 cut(s) 475
BsoBI CYCGRG 1 cut(s) 1225
BspACI CCGC 4 cut(s) 67, 126, 350, 450
BspANI GGCC 6 cut(s) 165, 489, 705, 949, 1269, 1290
BspCNI CTCAG 1 cut(s) 981
BspLI GGNNCC 1 cut(s) 256
BspPI GGATC 5 cut(s) 129, 780, 969, 1238, 1344
BspQI GCTCTTC 1 cut(s) 816
BspTI CTTAAG 3 cut(s) 659, 956, 980
BspTNI GGTCTC 1 cut(s) 475
BsrDI GCAATG 1 cut(s) 343
BsrFI RCCGGY 1 cut(s) 1290
BsrI ACTGG 1 cut(s) 30
BssAI RCCGGY 1 cut(s) 1290
BssECI CCNNGG 4 cut(s) 531, 1107, 1225, 1226
BssT1I CCWWGG 2 cut(s) 531, 1107
Bst2UI CCWGG 2 cut(s) 483, 951
Bst4CI ACNGT 1 cut(s) 905
Bst6I CTCTTC 2 cut(s) 816, 1037
BstAFI CTTAAG 3 cut(s) 659, 956, 980
BstC8I GCNNGC 2 cut(s) 452, 707
BstDEI CTNAG 1 cut(s) 989
BstF5I GGATG 2 cut(s) 166, 1355
BstH2I RGCGCY 1 cut(s) 225
BstHHI GCGC 2 cut(s) 224, 835
BstMAI GTCTC 3 cut(s) 374, 475, 1332
BstMWI GCNNNNNNNGC 3 cut(s) 451, 832, 1121
BstNI CCWGG 2 cut(s) 483, 951
BstSCI CCNGG 5 cut(s) 481, 949, 1224, 1225, 1358
BstSFI CTRYAG 1 cut(s) 1254
BstV1I GCAGC 6 cut(s) 211, 214, 359, 865, 868, 1102
BstX2I RGATCY 1 cut(s) 961
BstXI CCANNNNNNTGG 1 cut(s) 724
BstYI RGATCY 1 cut(s) 961
Bsu36I CCTNAGG 1 cut(s) 989
BsuI GTATCC 1 cut(s) 107
BsuRI GGCC 6 cut(s) 165, 489, 705, 949, 1269, 1290
BtsCI GGATG 2 cut(s) 166, 1355
BtsI GCAGTG 1 cut(s) 295
BtsIMutI CAGTG 1 cut(s) 295
Cac8I GCNNGC 2 cut(s) 452, 707
CaiI CAGNNNCTG 1 cut(s) 1207
CfoI GCGC 2 cut(s) 224, 835
Cfr10I RCCGGY 1 cut(s) 1290
Cfr13I GGNCC 5 cut(s) 255, 528, 683, 1267, 1288
Cfr9I CCCGGG 1 cut(s) 1225
CsiI ACCWGGT 1 cut(s) 481
Csp6I GTAC 3 cut(s) 234, 941, 1309
CviAII CATG 3 cut(s) 1153, 1331, 1343
CviQI GTAC 3 cut(s) 234, 941, 1309
DdeI CTNAG 1 cut(s) 989
DraI TTTAAA 1 cut(s) 505
DraIII CACNNNGTG 1 cut(s) 485
DriI GACNNNNNGTC 1 cut(s) 1027
EaeI YGGCCR 2 cut(s) 487, 703
Eam1104I CTCTTC 2 cut(s) 816, 1037
Eam1105I GACNNNNNGTC 1 cut(s) 1027
EarI CTCTTC 2 cut(s) 816, 1037
Eco130I CCWWGG 2 cut(s) 531, 1107
Eco147I AGGCCT 1 cut(s) 949
Eco31I GGTCTC 1 cut(s) 475
Eco32I GATATC 1 cut(s) 847
Eco47I GGWCC 3 cut(s) 255, 528, 683
Eco57I CTGAAG 2 cut(s) 752, 777
Eco81I CCTNAGG 1 cut(s) 989
Eco88I CYCGRG 1 cut(s) 1225
EcoO109I RGGNCCY 1 cut(s) 528
EcoRII CCWGG 2 cut(s) 481, 949
EcoRV GATATC 1 cut(s) 847
EcoT14I CCWWGG 2 cut(s) 531, 1107
ErhI CCWWGG 2 cut(s) 531, 1107
Esp3I CGTCTC 1 cut(s) 374
FaeI CATG 3 cut(s) 1156, 1334, 1346
FalI AAGNNNNNCTT 4 cut(s) 614, 646, 810, 842
FaqI GGGAC 3 cut(s) 313, 1154, 1208
FatI CATG 3 cut(s) 1152, 1330, 1342
FauI CCCGC 1 cut(s) 443
FbaI TGATCA 1 cut(s) 751
Fnu4HI GCNGC 7 cut(s) 67, 225, 228, 348, 879, 882, 1116
FokI GGATG 2 cut(s) 173, 1342
Fsp4HI GCNGC 7 cut(s) 67, 225, 228, 348, 879, 882, 1116
FspBI CTAG 5 cut(s) 96, 120, 231, 237, 885
GlaI GCGC 2 cut(s) 223, 834
GluI GCNGC 7 cut(s) 67, 225, 228, 348, 879, 882, 1116
GsaI CCCAGC 1 cut(s) 1289
GsuI CTGGAG 1 cut(s) 102
HaeII RGCGCY 1 cut(s) 225
HaeIII GGCC 6 cut(s) 165, 489, 705, 949, 1269, 1290
HapII CCGG 3 cut(s) 1226, 1291, 1360
HhaI GCGC 2 cut(s) 224, 835
Hin1II CATG 3 cut(s) 1156, 1334, 1346
Hin6I GCGC 2 cut(s) 222, 833
HinP1I GCGC 2 cut(s) 222, 833
HincII GTYRAC 1 cut(s) 271
HindII GTYRAC 1 cut(s) 271
HinfI GANTC 3 cut(s) 99, 141, 1304
HpaII CCGG 3 cut(s) 1226, 1291, 1360
HphI GGTGA 3 cut(s) 36, 331, 893
Hpy166II GTNNAC 3 cut(s) 271, 727, 901
Hpy188I TCNGA 5 cut(s) 146, 763, 796, 1033, 1209
Hpy188III TCNNGA 8 cut(s) 81, 96, 138, 328, 523, 740, 770, 1234
Hpy8I GTNNAC 3 cut(s) 271, 727, 901
HpyAV CCTTC 5 cut(s) 356, 379, 386, 390, 801
HpyCH4III ACNGT 1 cut(s) 905
HpyCH4V TGCA 5 cut(s) 479, 908, 1001, 1076, 1136
HpyF10VI GCNNNNNNNGC 3 cut(s) 451, 832, 1121
HpyF3I CTNAG 1 cut(s) 989
Hsp92II CATG 3 cut(s) 1156, 1334, 1346
HspAI GCGC 2 cut(s) 222, 833
Ksp22I TGATCA 1 cut(s) 751
LguI GCTCTTC 1 cut(s) 816
LmnI GCTCC 1 cut(s) 312
Lsp1109I GCAGC 6 cut(s) 211, 214, 359, 865, 868, 1102
LweI GCATC 1 cut(s) 421
MabI ACCWGGT 1 cut(s) 481
MaeI CTAG 5 cut(s) 96, 120, 231, 237, 885
MaeIII GTNAC 2 cut(s) 305, 1139
MboII GAAGA 7 cut(s) 415, 440, 634, 830, 833, 1001, 1054
MfeI CAATTG 2 cut(s) 755, 1262
MflI RGATCY 1 cut(s) 961
MlsI TGGCCA 1 cut(s) 705
MluNI TGGCCA 1 cut(s) 705
MmeI TCCRAC 3 cut(s) 357, 473, 710
Mox20I TGGCCA 1 cut(s) 705
MroXI GAANNNNTTC 3 cut(s) 366, 1008, 1320
MscI TGGCCA 1 cut(s) 705
MseI TTAA 8 cut(s) 441, 504, 660, 696, 936, 957, 981, 1239
MslI CAYNNNNRTG 1 cut(s) 722
Msp20I TGGCCA 1 cut(s) 705
MspA1I CMGCKG 1 cut(s) 350
MspCI CTTAAG 3 cut(s) 659, 956, 980
MspI CCGG 3 cut(s) 1226, 1291, 1360
MspR9I CCNGG 5 cut(s) 483, 951, 1226, 1227, 1360
MunI CAATTG 2 cut(s) 755, 1262
MvaI CCWGG 2 cut(s) 483, 951
MwoI GCNNNNNNNGC 3 cut(s) 451, 832, 1121
NciI CCSGG 3 cut(s) 1226, 1227, 1360
NlaIII CATG 3 cut(s) 1156, 1334, 1346
NlaIV GGNNCC 1 cut(s) 256
OliI CACNNNNGTG 1 cut(s) 722
PceI AGGCCT 1 cut(s) 949
PciSI GCTCTTC 1 cut(s) 816
PdmI GAANNNNTTC 3 cut(s) 366, 1008, 1320
PfeI GAWTC 3 cut(s) 99, 141, 1304
PflMI CCANNNNNTGG 1 cut(s) 1182
PfoI TCCNGGA 1 cut(s) 1358
PkrI GCNGC 7 cut(s) 68, 226, 229, 349, 880, 883, 1117
PpuMI RGGWCCY 1 cut(s) 528
PsiI TTATAA 1 cut(s) 566
Psp5II RGGWCCY 1 cut(s) 528
Psp6I CCWGG 2 cut(s) 481, 949
PspFI CCCAGC 1 cut(s) 1285
PspGI CCWGG 2 cut(s) 481, 949
PspN4I GGNNCC 1 cut(s) 256
PspPI GGNCC 5 cut(s) 255, 528, 683, 1267, 1288
PspPPI RGGWCCY 1 cut(s) 528
PstNI CAGNNNCTG 1 cut(s) 1207
PsuI RGATCY 1 cut(s) 961
RsaI GTAC 3 cut(s) 235, 942, 1310
RsaNI GTAC 3 cut(s) 234, 941, 1309
RseI CAYNNNNRTG 1 cut(s) 722
SapI GCTCTTC 1 cut(s) 816
SaqAI TTAA 8 cut(s) 441, 504, 660, 696, 936, 957, 981, 1239
SatI GCNGC 7 cut(s) 67, 225, 228, 348, 879, 882, 1116
Sau96I GGNCC 5 cut(s) 255, 528, 683, 1267, 1288
ScaI AGTACT 1 cut(s) 235
ScrFI CCNGG 5 cut(s) 483, 951, 1226, 1227, 1360
SexAI ACCWGGT 1 cut(s) 481
SfaNI GCATC 1 cut(s) 421
SfcI CTRYAG 1 cut(s) 1254
SinI GGWCC 3 cut(s) 255, 528, 683
SmaI CCCGGG 1 cut(s) 1227
SmiMI CAYNNNNRTG 1 cut(s) 722
SmlI CTYRAG 5 cut(s) 631, 659, 738, 956, 980
SmoI CTYRAG 5 cut(s) 631, 659, 738, 956, 980
SpeI ACTAGT 1 cut(s) 236
SseBI AGGCCT 1 cut(s) 949
SsiI CCGC 4 cut(s) 67, 126, 350, 450
SspI AATATT 4 cut(s) 409, 670, 973, 1367
SspMI CTAG 5 cut(s) 96, 120, 231, 237, 885
StuI AGGCCT 1 cut(s) 949
StyD4I CCNGG 5 cut(s) 481, 949, 1224, 1225, 1358
StyI CCWWGG 2 cut(s) 531, 1107
TaaI ACNGT 1 cut(s) 905
TaqI TCGA 6 cut(s) 133, 204, 281, 465, 515, 1348
TaqII GACCGA 1 cut(s) 256
TatI WGTACW 2 cut(s) 233, 940
TauI GCSGC 1 cut(s) 69
TfiI GAWTC 3 cut(s) 99, 141, 1304
Tru1I TTAA 8 cut(s) 441, 504, 660, 696, 936, 957, 981, 1239
Tru9I TTAA 8 cut(s) 441, 504, 660, 696, 936, 957, 981, 1239
TscAI CASTG 1 cut(s) 295
TseI GCWGC 6 cut(s) 224, 227, 347, 878, 881, 1115
TspDTI ATGAA 4 cut(s) 550, 682, 882, 1331
TspMI CCCGGG 1 cut(s) 1225
TspRI CASTG 1 cut(s) 295
Van91I CCANNNNNTGG 1 cut(s) 1182
Vha464I CTTAAG 3 cut(s) 659, 956, 980
VpaK11BI GGWCC 3 cut(s) 255, 528, 683
XapI RAATTY 3 cut(s) 557, 590, 1320
XbaI TCTAGA 1 cut(s) 95
XcmI CCANNNNNNNNNTGG 1 cut(s) 1179
XmaI CCCGGG 1 cut(s) 1225
XmnI GAANNNNTTC 3 cut(s) 366, 1008, 1320
XspI CTAG 5 cut(s) 96, 120, 231, 237, 885
ZrmI AGTACT 1 cut(s) 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.