RLG00000018244

aarF domain-containing protein kinase At1g79600

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0007840
Physical Location & Seq
Reverse (-)
27497789 .. 27510477
12689 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018244

Sequence Viewer

Length: 2235 bp
ATGGCGTCCTGTTCGTTACCGTTACCGGAGCTCACCTTTCTCTCGCCGCGGACGACTTCTAAACGCCGGCTTTCTCTCTCCCGAGTTCCTCTCTCTAAATTTCCTATTTCCAGATACCAGAGGTGCGGTCTCCGATCGCGAATCAGAGCGGCGAAAGAGGAAGGGGTGGTGGTGGCGGTGGAAGAGAGGGAAGCGGAGTTGATCAAGAATGTGAATGGCGTTAGTGGTAGTGGATATAGTTCGAATGGCGCGGCGAACGAGTACTCGAATGGAAGCGTGAGGGTGGTGGAGAGTGAGAATGGGAGCTTGGTGAAGTATGTGAATGGGAATGGGGCGGCGGAGGCCACGGTGGTGGATGAGGTTGAGGTTTTGAAGTCCAAGGAGGAAGGGAGGCAGAGGAAGATTGAGGAAATTGGTAAGGAGGACGCGTGGTTTAAGCAAAGCGGGCTGCCAGAGGTTGAGGTTTCTGTTGCACCTGGTGGCCGTTGGAGTAGATTCAAAACTTACTCGACAATCCAGAGGACCTTGGAAATATGGGGATTTGTTCTATCTTTCCTCGTTAGGGCTTGGTGGAATACTAAAAAATTTGCCTATCGAGGGGGAATGACAGAGGAAAAGCAAACCTTAAGGCGTAAGGCGCTTGCCAAGTGGCTAAAGGAGAATATTCTGAGATTGGGTCCAACATTCATCAAAATTGGCCAGCAGTTCTCCACAAGGGTCGACATTCTCGCTCAAGAATATGTTGACCAGTTGTCTGAACTTCAGGATCAAGTTCCTCCTTTCCCATCAGAAACAGCTGTATCAATCGTTGAAGAAGAGCTTGGAGCTCCTGTGAATGCTATCTTTGATCGTTTTGACTATGAACCAATAGCTGCTGCTAGTCTTGGTCAGGTTCACCGTGCAAGGTTGAAGGGACAGGAAGTTGTTATTAAAGTACAGAGGCCTGGACTCAAGGATCTTTTCAATATTGATCTTAAGAACCTGAGGGTAATTGCAGAATATCTTCAGAAAATTGACCCCAAGTCCGATGGTGCGAAGAGGGATTGGGTTGCTATCTATGATGAGTGTGCTAATGTGTTATATCAGGAGATTGATTACACAAAGGAAGCTGATAATGCTGAACTATTTGCAAGTAACTTCAAGAATATGGATTATGTGAAAGTCCCAACAATAGTACGGGAATACACCACACCACAGGTTCTGACTATGGAATATGTCCCTGGGATCAAAATAAACAAGATACAAGCTTTGGATCAATTGGGAGTTGACCGGCAGAGGCTGGGACGGTACGCTGTTGAATCATACTTGGAACAAATTCTGTCTCATGGGTTTTTCCATGCTGATCCTCATCCTGGAAATATTGCTGTGGATGATGTCAATGGTGGAAGATTGATCTTTTACGATTTTGGAATGATGGGAAGCATCAGTCCAAACATTAGAGAAGGTTTGCTGGAAACATTTTATGGAGTTTATGAGAAGGATCCAGATAAGGTCCTTCAGGCAATGATTCAAATGGGTGTTCTTGTGCCTACTGGAGATATGACAGCTGTCAGACGAACAGCACAGTTCTTCCTTAGGAGTTTTGAAGAGCGTCTTGCTGCACAGAGAAAAGAGAGAGAAATGGCAACAAAAGAACTTGGATTTAAAAAGCCACTGAGCAAGGAGGAAAGGATAATGAAAAAGAAGGAAAGGCTGGCTGCAATTGGGGAAGATTTATTAGCCATTGCAGCAGACCAACCCTTCAGATTCCCAGCGACATTCACATTCGTTGTAAGAGCATTTTCAGTGTTGGATGGAATCGGGAAGGGTCTTGATCCTCGATTTGATATCACTGAGATTGCCAAACCGTATGCACTGGAATTGCTAAAATTTCGTGAAGCTGGAGTTGAAGTCGTTTGGAAGGACATCAGAAAGCGATGGGACAGGCAATCTCAGGCATTTTATAACTTATTTAGACAGGCAGACAGAGTTGAAAAGCTTGCTGAAATTATCCAAAGATTGGAGCAAGGTGATCTGAAGCTTAGAGTTCGGACTTTAGAATCTGAGAGGGCATTCCAGCGAGTTGCTACTGTCCAGAAGACTGTTGGAAATGCTGTTGCTGCTGGAAGCCTGATCAACCTCGCAACAATCTTGTATATTAATTCCATTCGAGTGCCTGCCTTTGGACTGCTTAATCAATTGGAGATCCAGCTCATGCTTTTACGTGTCAACCTTCCAATCCTTGAAGAAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC1

Protein Analysis

745

Amino Acids

84.29

Weight (kDa)

8.85

Isoelectric Point (pI)

41.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC1 PF03109 254 - 501 8.4e-85 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1944
AccB7I CCANNNNNTGG 1 cut(s) 1999
AccBSI CCGCTC 1 cut(s) 149
AccI GTMKAC 1 cut(s) 720
AccII CGCG 4 cut(s) 49, 139, 251, 428
AclWI GGATC 9 cut(s) 774, 963, 1232, 1260, 1337, 1475, 1488, 1808, 2179
AcoI YGGCCR 2 cut(s) 481, 697
AcsI RAATTY 4 cut(s) 98, 584, 1314, 1868
AcuI CTGAAG 5 cut(s) 746, 989, 1481, 1726, 2036
AcyI GRCGYC 1 cut(s) 5
AdeI CACNNNGTG 1 cut(s) 479
AfaI GTAC 4 cut(s) 263, 936, 1176, 1289
AfiI CCNNNNNNNGG 5 cut(s) 562, 597, 1352, 1999, 2162
AflII CTTAAG 2 cut(s) 625, 974
AflIII ACRYGT 2 cut(s) 426, 2203
AhdI GACNNNNNGTC 2 cut(s) 751, 1021
AjnI CCWGG 4 cut(s) 475, 943, 1219, 1351
AjuI GAANNNNNNNTTGG 4 cut(s) 290, 322, 804, 836
AleI CACNNNNGTG 1 cut(s) 350
Alw21I GWGCWC 2 cut(s) 33, 829
Alw26I GTCTC 2 cut(s) 134, 1326
AlwI GGATC 9 cut(s) 774, 963, 1232, 1260, 1337, 1475, 1488, 1808, 2179
AlwNI CAGNNNCTG 2 cut(s) 1201, 1279
Ama87I CYCGRG 1 cut(s) 81
AoxI GGCC 4 cut(s) 342, 481, 697, 941
ApeKI GCWGC 7 cut(s) 448, 872, 875, 1598, 1697, 1727, 2099
ApoI RAATTY 4 cut(s) 98, 584, 1314, 1868
AseI ATTAAT 1 cut(s) 2139
Asp700I GAANNNNTTC 2 cut(s) 1002, 1314
AspLEI GCGC 2 cut(s) 251, 640
AspS9I GGNCC 3 cut(s) 522, 677, 1492
AsuHPI GGTGA 4 cut(s) 25, 322, 887, 2021
AsuII TTCGAA 1 cut(s) 242
AvaI CYCGRG 1 cut(s) 81
AvaII GGWCC 3 cut(s) 522, 677, 1492
AxyI CCTNAGG 2 cut(s) 983, 1574
BaeI ACNNNNGTAYC 2 cut(s) 783, 816
BalI TGGCCA 1 cut(s) 699
BamHI GGATCC 1 cut(s) 1480
BanII GRGCYC 2 cut(s) 33, 829
BbsI GAAGAC 1 cut(s) 2084
Bbv12I GWGCWC 2 cut(s) 33, 829
BbvI GCAGC 7 cut(s) 435, 859, 862, 1585, 1684, 1739, 2086
BccI CCATC 5 cut(s) 793, 1022, 1408, 1787, 1911
BceAI ACGGC 1 cut(s) 468
BciT130I CCWGG 4 cut(s) 477, 945, 1221, 1353
BclI TGATCA 2 cut(s) 201, 2112
BcoDI GTCTC 2 cut(s) 134, 1326
BfaI CTAG 1 cut(s) 879
BfoI RGCGCY 1 cut(s) 641
BfrI CTTAAG 2 cut(s) 625, 974
BmcAI AGTACT 1 cut(s) 263
Bme1390I CCNGG 4 cut(s) 477, 945, 1221, 1353
Bme18I GGWCC 3 cut(s) 522, 677, 1492
BmeRI GACNNNNNGTC 2 cut(s) 751, 1021
BmeT110I CYCGRG 1 cut(s) 81
BmgT120I GGNCC 3 cut(s) 522, 677, 1492
BmiI GGNNCC 2 cut(s) 678, 1482
BmrFI CCNGG 4 cut(s) 477, 945, 1221, 1353
BmsI GCATC 1 cut(s) 1431
BoxI GACNNNNGTC 1 cut(s) 1547
BpiI GAAGAC 1 cut(s) 2084
BplI GAGNNNNNCTC 2 cut(s) 75, 107
BpmI CTGGAG 2 cut(s) 1554, 1902
Bpu14I TTCGAA 1 cut(s) 242
BpuEI CTTGAG 2 cut(s) 717, 935
BsaAI YACGTR 1 cut(s) 2204
BsaBI GATNNNNATC 1 cut(s) 1347
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 134
BsaJI CCNNGG 6 cut(s) 47, 345, 378, 525, 1219, 1220
BsaWI WCCGGW 1 cut(s) 25
BsaXI ACNNNNNCTCC 6 cut(s) 413, 443, 816, 846, 1875, 1905
Bsc4I CCNNNNNNNGG 5 cut(s) 562, 597, 1352, 1999, 2162
Bse118I RCCGGY 2 cut(s) 66, 1269
Bse1I ACTGG 3 cut(s) 748, 1537, 1860
Bse21I CCTNAGG 2 cut(s) 983, 1574
Bse3DI GCAATG 2 cut(s) 1509, 1722
Bse8I GATNNNNATC 1 cut(s) 1347
BseBI CCWGG 4 cut(s) 477, 945, 1221, 1353
BseDI CCNNGG 6 cut(s) 47, 345, 378, 525, 1219, 1220
BseGI GGATG 4 cut(s) 361, 1348, 1375, 1798
BseJI GATNNNNATC 1 cut(s) 1347
BseLI CCNNNNNNNGG 5 cut(s) 562, 597, 1352, 1999, 2162
BseMI GCAATG 2 cut(s) 1509, 1722
BseMII CTCAG 6 cut(s) 659, 974, 1646, 1824, 1946, 2034
BseNI ACTGG 3 cut(s) 748, 1537, 1860
BseXI GCAGC 7 cut(s) 435, 859, 862, 1585, 1684, 1739, 2086
BseYI CCCAGC 2 cut(s) 1279, 1750
BsgI GTGCAG 1 cut(s) 1584
Bsh1236I CGCG 4 cut(s) 49, 139, 251, 428
Bsh1285I CGRYCG 1 cut(s) 137
BshFI GGCC 4 cut(s) 344, 483, 699, 943
BsiEI CGRYCG 1 cut(s) 137
BsiHKAI GWGCWC 2 cut(s) 33, 829
BsiHKCI CYCGRG 1 cut(s) 81
BsiSI CCGG 3 cut(s) 26, 67, 1270
BslFI GGGAC 5 cut(s) 927, 1148, 1202, 1296, 1934
BslI CCNNNNNNNGG 5 cut(s) 562, 597, 1352, 1999, 2162
BsmAI GTCTC 2 cut(s) 134, 1326
BsmFI GGGAC 5 cut(s) 927, 1148, 1202, 1296, 1934
BsmI GAATGC 2 cut(s) 841, 2051
BsnI GGCC 4 cut(s) 344, 483, 699, 943
Bso31I GGTCTC 1 cut(s) 134
BsoBI CYCGRG 1 cut(s) 81
Bsp119I TTCGAA 1 cut(s) 242
Bsp1286I GDGCHC 2 cut(s) 33, 829
Bsp68I TCGCGA 1 cut(s) 139
BspANI GGCC 4 cut(s) 344, 483, 699, 943
BspCNI CTCAG 6 cut(s) 660, 975, 1647, 1825, 1945, 2035
BspFNI CGCG 4 cut(s) 49, 139, 251, 428
BspLI GGNNCC 2 cut(s) 678, 1482
BspPI GGATC 9 cut(s) 774, 963, 1232, 1260, 1337, 1475, 1488, 1808, 2179
BspQI GCTCTTC 2 cut(s) 810, 1581
BspT104I TTCGAA 1 cut(s) 242
BspTI CTTAAG 2 cut(s) 625, 974
BspTNI GGTCTC 1 cut(s) 134
BsrBI CCGCTC 1 cut(s) 149
BsrDI GCAATG 2 cut(s) 1509, 1722
BsrFI RCCGGY 2 cut(s) 66, 1269
BsrI ACTGG 3 cut(s) 748, 1537, 1860
BssAI RCCGGY 2 cut(s) 66, 1269
BssECI CCNNGG 6 cut(s) 47, 345, 378, 525, 1219, 1220
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 2 cut(s) 378, 525
Bst2UI CCWGG 4 cut(s) 477, 945, 1221, 1353
Bst4CI ACNGT 8 cut(s) 21, 349, 899, 1287, 1566, 1848, 2071, 2083
Bst6I CTCTTC 4 cut(s) 177, 810, 1031, 1581
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 2 cut(s) 625, 974
BstBAI YACGTR 1 cut(s) 2204
BstBI TTCGAA 1 cut(s) 242
BstC8I GCNNGC 7 cut(s) 68, 446, 642, 701, 1695, 1980, 2157
BstDEI CTNAG 8 cut(s) 668, 983, 1574, 1655, 1833, 1932, 2021, 2043
BstDSI CCRYGG 2 cut(s) 47, 345
BstF5I GGATG 4 cut(s) 361, 1348, 1375, 1798
BstFNI CGCG 4 cut(s) 49, 139, 251, 428
BstH2I RGCGCY 1 cut(s) 641
BstHHI GCGC 2 cut(s) 251, 640
BstMAI GTCTC 2 cut(s) 134, 1326
BstMCI CGRYCG 1 cut(s) 137
BstMWI GCNNNNNNNGC 6 cut(s) 341, 445, 637, 1115, 1727, 2099
BstNI CCWGG 4 cut(s) 477, 945, 1221, 1353
BstPAI GACNNNNGTC 1 cut(s) 1547
BstSCI CCNGG 4 cut(s) 475, 943, 1219, 1351
BstUI CGCG 4 cut(s) 49, 139, 251, 428
BstV1I GCAGC 7 cut(s) 435, 859, 862, 1585, 1684, 1739, 2086
BstV2I GAAGAC 1 cut(s) 2084
BstX2I RGATCY 3 cut(s) 955, 1480, 2184
BstXI CCANNNNNNTGG 1 cut(s) 352
BstYI RGATCY 3 cut(s) 955, 1480, 2184
Bsu36I CCTNAGG 2 cut(s) 983, 1574
BsuRI GGCC 4 cut(s) 344, 483, 699, 943
BtgI CCRYGG 2 cut(s) 47, 345
BtgZI GCGATG 1 cut(s) 1930
BtsCI GGATG 4 cut(s) 361, 1348, 1375, 1798
BtsIMutI CAGTG 4 cut(s) 1652, 1791, 1830, 1853
BtuMI TCGCGA 1 cut(s) 139
Cac8I GCNNGC 7 cut(s) 68, 446, 642, 701, 1695, 1980, 2157
CaiI CAGNNNCTG 2 cut(s) 1201, 1279
CfoI GCGC 2 cut(s) 251, 640
Cfr10I RCCGGY 2 cut(s) 66, 1269
Cfr13I GGNCC 3 cut(s) 522, 677, 1492
Cfr42I CCGCGG 1 cut(s) 50
CseI GACGC 2 cut(s) 434, 1580
CsiI ACCWGGT 1 cut(s) 475
Csp6I GTAC 4 cut(s) 262, 935, 1175, 1288
CviAII CATG 3 cut(s) 1325, 1337, 2194
CviQI GTAC 4 cut(s) 262, 935, 1175, 1288
DdeI CTNAG 8 cut(s) 668, 983, 1574, 1655, 1833, 1932, 2021, 2043
DraI TTTAAA 1 cut(s) 1645
DraIII CACNNNGTG 1 cut(s) 479
DriI GACNNNNNGTC 2 cut(s) 751, 1021
EaeI YGGCCR 2 cut(s) 481, 697
Eam1104I CTCTTC 4 cut(s) 177, 810, 1031, 1581
Eam1105I GACNNNNNGTC 2 cut(s) 751, 1021
EarI CTCTTC 4 cut(s) 177, 810, 1031, 1581
EciI GGCGGA 1 cut(s) 353
Ecl136II GAGCTC 2 cut(s) 31, 827
Eco130I CCWWGG 2 cut(s) 378, 525
Eco147I AGGCCT 1 cut(s) 943
Eco24I GRGCYC 2 cut(s) 33, 829
Eco31I GGTCTC 1 cut(s) 134
Eco32I GATATC 1 cut(s) 1828
Eco47I GGWCC 3 cut(s) 522, 677, 1492
Eco53kI GAGCTC 2 cut(s) 31, 827
Eco57I CTGAAG 5 cut(s) 746, 989, 1481, 1726, 2036
Eco81I CCTNAGG 2 cut(s) 983, 1574
Eco88I CYCGRG 1 cut(s) 81
EcoICRI GAGCTC 2 cut(s) 31, 827
EcoO109I RGGNCCY 2 cut(s) 522, 1492
EcoRII CCWGG 4 cut(s) 475, 943, 1219, 1351
EcoRV GATATC 1 cut(s) 1828
EcoT14I CCWWGG 2 cut(s) 378, 525
EcoT38I GRGCYC 2 cut(s) 33, 829
ErhI CCWWGG 2 cut(s) 378, 525
FaeI CATG 3 cut(s) 1328, 1340, 2197
FalI AAGNNNNNCTT 6 cut(s) 608, 640, 804, 836, 1578, 1610
FaqI GGGAC 5 cut(s) 927, 1148, 1202, 1296, 1934
FatI CATG 3 cut(s) 1324, 1336, 2193
FauI CCCGC 1 cut(s) 437
FbaI TGATCA 2 cut(s) 201, 2112
FblI GTMKAC 1 cut(s) 720
FokI GGATG 4 cut(s) 368, 1335, 1382, 1805
FriOI GRGCYC 2 cut(s) 33, 829
FspBI CTAG 1 cut(s) 879
GlaI GCGC 2 cut(s) 250, 639
GsaI CCCAGC 2 cut(s) 1283, 1754
GsuI CTGGAG 2 cut(s) 1554, 1902
HaeII RGCGCY 1 cut(s) 641
HaeIII GGCC 4 cut(s) 344, 483, 699, 943
HapII CCGG 3 cut(s) 26, 67, 1270
HgaI GACGC 2 cut(s) 434, 1580
HhaI GCGC 2 cut(s) 251, 640
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 3 cut(s) 1328, 1340, 2197
Hin6I GCGC 2 cut(s) 249, 638
HinP1I GCGC 2 cut(s) 249, 638
HincII GTYRAC 4 cut(s) 721, 745, 1267, 2209
HindII GTYRAC 4 cut(s) 721, 745, 1267, 2209
HindIII AAGCTT 3 cut(s) 1245, 1976, 2018
HinfI GANTC 8 cut(s) 141, 495, 948, 1298, 1507, 1746, 1797, 2039
HpaII CCGG 3 cut(s) 26, 67, 1270
HphI GGTGA 4 cut(s) 25, 322, 887, 2021
Hpy166II GTNNAC 5 cut(s) 721, 745, 895, 1267, 2209
Hpy8I GTNNAC 5 cut(s) 721, 745, 895, 1267, 2209
HpyCH4III ACNGT 8 cut(s) 21, 349, 899, 1287, 1566, 1848, 2071, 2083
HpyCH4IV ACGT 1 cut(s) 2203
HpyCH4V TGCA 8 cut(s) 473, 902, 995, 1130, 1601, 1700, 1727, 1853
HpyF10VI GCNNNNNNNGC 6 cut(s) 341, 445, 637, 1115, 1727, 2099
HpyF3I CTNAG 8 cut(s) 668, 983, 1574, 1655, 1833, 1932, 2021, 2043
HpySE526I ACGT 1 cut(s) 2203
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 3 cut(s) 1328, 1340, 2197
HspAI GCGC 2 cut(s) 249, 638
KroI GCCGGC 1 cut(s) 66
KroNI GCCGGC 1 cut(s) 68
Ksp22I TGATCA 2 cut(s) 201, 2112
KspI CCGCGG 1 cut(s) 50
LguI GCTCTTC 2 cut(s) 810, 1581
LmnI GCTCC 5 cut(s) 28, 303, 824, 832, 2002
Lsp1109I GCAGC 7 cut(s) 435, 859, 862, 1585, 1684, 1739, 2086
LweI GCATC 1 cut(s) 1431
MabI ACCWGGT 1 cut(s) 475
MaeI CTAG 1 cut(s) 879
MaeII ACGT 1 cut(s) 2203
MaeIII GTNAC 3 cut(s) 15, 21, 1133
MbiI CCGCTC 1 cut(s) 149
MfeI CAATTG 3 cut(s) 1256, 1701, 2177
MflI RGATCY 3 cut(s) 955, 1480, 2184
MhlI GDGCHC 2 cut(s) 33, 829
MlsI TGGCCA 1 cut(s) 699
MluI ACGCGT 1 cut(s) 426
MluNI TGGCCA 1 cut(s) 699
MlyI GAGTC 1 cut(s) 942
MmeI TCCRAC 4 cut(s) 467, 704, 1770, 2065
Mox20I TGGCCA 1 cut(s) 699
MroNI GCCGGC 1 cut(s) 66
MroXI GAANNNNTTC 2 cut(s) 1002, 1314
MscI TGGCCA 1 cut(s) 699
MseI TTAA 7 cut(s) 435, 626, 930, 975, 1644, 2139, 2172
MslI CAYNNNNRTG 2 cut(s) 350, 2150
Msp20I TGGCCA 1 cut(s) 699
MspA1I CMGCKG 3 cut(s) 49, 797, 1547
MspCI CTTAAG 2 cut(s) 625, 974
MspI CCGG 3 cut(s) 26, 67, 1270
MspR9I CCNGG 4 cut(s) 477, 945, 1221, 1353
MunI CAATTG 3 cut(s) 1256, 1701, 2177
Mva1269I GAATGC 2 cut(s) 841, 2051
MvaI CCWGG 4 cut(s) 477, 945, 1221, 1353
MvnI CGCG 4 cut(s) 49, 139, 251, 428
MwoI GCNNNNNNNGC 6 cut(s) 341, 445, 637, 1115, 1727, 2099
NaeI GCCGGC 1 cut(s) 68
NgoMIV GCCGGC 1 cut(s) 66
NlaIII CATG 3 cut(s) 1328, 1340, 2197
NlaIV GGNNCC 2 cut(s) 678, 1482
NruI TCGCGA 1 cut(s) 139
NspV TTCGAA 1 cut(s) 242
OliI CACNNNNGTG 1 cut(s) 350
PasI CCCWGGG 1 cut(s) 1220
PceI AGGCCT 1 cut(s) 943
PciSI GCTCTTC 2 cut(s) 810, 1581
PcsI WCGNNNNNNNCGW 1 cut(s) 50
PctI GAATGC 2 cut(s) 841, 2051
PdiI GCCGGC 1 cut(s) 68
PdmI GAANNNNTTC 2 cut(s) 1002, 1314
PfeI GAWTC 7 cut(s) 141, 495, 1298, 1507, 1746, 1797, 2039
PflMI CCANNNNNTGG 1 cut(s) 1999
PfoI TCCNGGA 1 cut(s) 1351
Ple19I CGATCG 1 cut(s) 137
PleI GAGTC 1 cut(s) 942
PpsI GAGTC 1 cut(s) 942
Ppu21I YACGTR 1 cut(s) 2204
PpuMI RGGWCCY 2 cut(s) 522, 1492
PshAI GACNNNNGTC 1 cut(s) 1547
PshBI ATTAAT 1 cut(s) 2139
PsiI TTATAA 1 cut(s) 1944
Psp124BI GAGCTC 2 cut(s) 33, 829
Psp5II RGGWCCY 2 cut(s) 522, 1492
Psp6I CCWGG 4 cut(s) 475, 943, 1219, 1351
PspFI CCCAGC 2 cut(s) 1279, 1750
PspGI CCWGG 4 cut(s) 475, 943, 1219, 1351
PspN4I GGNNCC 2 cut(s) 678, 1482
PspPI GGNCC 3 cut(s) 522, 677, 1492
PspPPI RGGWCCY 2 cut(s) 522, 1492
PstNI CAGNNNCTG 2 cut(s) 1201, 1279
PsuI RGATCY 3 cut(s) 955, 1480, 2184
PvuI CGATCG 1 cut(s) 137
PvuII CAGCTG 2 cut(s) 797, 1547
RruI TCGCGA 1 cut(s) 139
RsaI GTAC 4 cut(s) 263, 936, 1176, 1289
RsaNI GTAC 4 cut(s) 262, 935, 1175, 1288
RseI CAYNNNNRTG 2 cut(s) 350, 2150
SacI GAGCTC 2 cut(s) 33, 829
SacII CCGCGG 1 cut(s) 50
SalI GTCGAC 1 cut(s) 719
SapI GCTCTTC 2 cut(s) 810, 1581
SaqAI TTAA 7 cut(s) 435, 626, 930, 975, 1644, 2139, 2172
Sau96I GGNCC 3 cut(s) 522, 677, 1492
ScaI AGTACT 1 cut(s) 263
SchI GAGTC 1 cut(s) 942
ScrFI CCNGG 4 cut(s) 477, 945, 1221, 1353
SduI GDGCHC 2 cut(s) 33, 829
SexAI ACCWGGT 1 cut(s) 475
SfaNI GCATC 1 cut(s) 1431
Sfr303I CCGCGG 1 cut(s) 50
SfuI TTCGAA 1 cut(s) 242
SgrBI CCGCGG 1 cut(s) 50
SinI GGWCC 3 cut(s) 522, 677, 1492
SmiMI CAYNNNNRTG 2 cut(s) 350, 2150
SmlI CTYRAG 4 cut(s) 625, 732, 950, 974
SmoI CTYRAG 4 cut(s) 625, 732, 950, 974
SseBI AGGCCT 1 cut(s) 943
SspI AATATT 3 cut(s) 664, 967, 1360
SspMI CTAG 1 cut(s) 879
SstI GAGCTC 2 cut(s) 33, 829
StuI AGGCCT 1 cut(s) 943
StyD4I CCNGG 4 cut(s) 475, 943, 1219, 1351
StyI CCWWGG 2 cut(s) 378, 525
TaaI ACNGT 8 cut(s) 21, 349, 899, 1287, 1566, 1848, 2071, 2083
TaiI ACGT 1 cut(s) 2206
TaqI TCGA 7 cut(s) 242, 266, 509, 595, 720, 1819, 2149
TatI WGTACW 2 cut(s) 261, 934
TauI GCSGC 4 cut(s) 49, 152, 254, 338
TfiI GAWTC 7 cut(s) 141, 495, 1298, 1507, 1746, 1797, 2039
Tru1I TTAA 7 cut(s) 435, 626, 930, 975, 1644, 2139, 2172
Tru9I TTAA 7 cut(s) 435, 626, 930, 975, 1644, 2139, 2172
TscAI CASTG 4 cut(s) 1659, 1791, 1837, 1860
TseI GCWGC 7 cut(s) 448, 872, 875, 1598, 1697, 1727, 2099
TspDTI ATGAA 3 cut(s) 676, 876, 1691
TspRI CASTG 4 cut(s) 1659, 1791, 1837, 1860
Van91I CCANNNNNTGG 1 cut(s) 1999
Vha464I CTTAAG 2 cut(s) 625, 974
VpaK11BI GGWCC 3 cut(s) 522, 677, 1492
VspI ATTAAT 1 cut(s) 2139
XapI RAATTY 4 cut(s) 98, 584, 1314, 1868
XcmI CCANNNNNNNNNTGG 1 cut(s) 2081
XmiI GTMKAC 1 cut(s) 720
XmnI GAANNNNTTC 2 cut(s) 1002, 1314
XspI CTAG 1 cut(s) 879
ZrmI AGTACT 1 cut(s) 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.