Rroxscaffold_2G00127450

aarF domain-containing protein kinase At1g79600

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0007840
Physical Location & Seq
Forward (+)
62897676 .. 62910944
13269 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00127450.1

Sequence Viewer

Length: 1653 bp
ATGACAGAGGAAAAGCAAACCTTAAGGCGTAAGGCGCTTGCCAAGTGGCTAAAGGAGAATATTCTGAGATTGGGTCCAACATTCATCAAAATTGGCCAGCAGTTCTCCACAAGGGTTGACATTCTCGCTCAAGAATATGTTGATCAGTTGTCTGAACTTCAGGATCAAGTTCCTCCTTTCCCATCAGAAACAGCTGTATCAATTGTTGAAGAAGAGCTTGGAGCTCCTGTGAATGCTATCTTTGATCGTTTTGACTATGAACCAATAGCTGCTGCTAGTCTTGGTCAGGTTCACCGTGCAAGGTTGAAGGGACAGGAAGTTGTTATTAAAGTACAAAGGCCTGGACTCAAGGATCTTTTCAATATTGATCTTAAGAACCTGAGGGTAATTGCAGAATATCTTCAGAAAATTGACCCCAAGTCAGATGGTGCGAAGAGGGATTGGGTTGCTATCTATGATGAGTGTGCTAATGTGTTATATCAGGAGATTGATTACACAAAGGAAGCTGATAATGCTGAACTATTTGCAAGTAACTTCAAGAATATGGATTATGTGAAAGTCCCAACAATAGTACGGGAATACACCACACCACAGGTTCTGACTATGGAATATGTCCCTGGGATCAAAATAAACAAGATACAAGCTTTGGATCAATTGGGAGTTGACCGGCAGAGGCTGGGTCGGTACGCTGTTGAATCATACTTGGAACAAATTCTGTCTCATGGGTTTTTCCATGCTGATCCTCATCCTGGAAATATTGCTGTGGATGATGTCAATGGTGGAAGATTGATCTTTTACGATTTTGGAATGATGGGAAGCATCAGTCCAAACATTAGAGAAGGTTTGCTGGAAACATTTTATGGAGTTTATGAGAAGGATCCAGATAAGGTCCTTCAGGCAATGATTCAAATGGGTGTTCTTGTGCCTACTGGAGATATGACAGCTGTCAGACGAACAGCACAGTTCTTCCTTAGGAGTTTTGAAGAGCGTCTTGCTGCACAGAGAAAAGAGAGAGAAATGGCAACAAAAGAACTTGGATTTAAAAAGCCACTGAGCAAGGAGGAAAGGATAATGAAAAAGAAGGAAAGGCTGGCTGCAATTGGGGAAGATTTATTAGCCATTGCAGCAGACCAACCCTTCAGATTCCCTGCGACATTCACATTCGTTGTAAGAGCATTTTCAGTGTTGGATGGAATCGGGAAGGGTCTTGACCCTCGATTTGATATCACTGAGATTGCCAAACCGTATGCACTGGAATTGCTAAAATTTCGTGAAGCTGGAGTTGAAGACATCAGAAAGCGATGGGACAGGCAATCTCAGGCATTTTATAACTTATTTAGACAGGCAGACAGAGTTGAAAAGCTTGCTGAAATTATCCAAAGATTGGAGCAAGGTGATCTGAAGCTTAGAGTTCGGACTTTAGAATCTGAGAGGGCGTTCCAGCGAGTTGCTACTGTCCAGAAGACTGTTGGAAATGCTGTTGCTGCTGGAAGCCTGATCAACCTCGCAACAATCTTGTATATTAATTCCATTCGAGTGCCTGCCATTGTAGCATATATCTCTTGTGCATTTTTTTGCGTTCAAGTTCTTATTGGCATCATAAAGGTCAAAAAGTTTGATGAACGGGAGCGATTGATAACAGGAACTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC1

Protein Analysis

550

Amino Acids

62.6

Weight (kDa)

8.14

Isoelectric Point (pI)

38.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC1 PF03109 53 - 300 4.3e-85 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1329
AccB7I CCANNNNNTGG 1 cut(s) 1384
AclWI GGATC 7 cut(s) 171, 360, 629, 657, 734, 872, 885
AcoI YGGCCR 1 cut(s) 94
AcsI RAATTY 2 cut(s) 711, 1265
AcuI CTGAAG 5 cut(s) 143, 386, 878, 1123, 1421
AfaI GTAC 3 cut(s) 333, 573, 686
AfiI CCNNNNNNNGG 2 cut(s) 749, 1384
AflII CTTAAG 2 cut(s) 22, 371
AhdI GACNNNNNGTC 1 cut(s) 418
AjnI CCWGG 3 cut(s) 340, 616, 748
AjuI GAANNNNNNNTTGG 2 cut(s) 201, 233
Alw21I GWGCWC 1 cut(s) 226
Alw26I GTCTC 1 cut(s) 723
AlwI GGATC 7 cut(s) 171, 360, 629, 657, 734, 872, 885
AlwNI CAGNNNCTG 3 cut(s) 598, 676, 1646
AoxI GGCC 2 cut(s) 94, 338
ApeKI GCWGC 6 cut(s) 269, 272, 995, 1094, 1124, 1484
ApoI RAATTY 2 cut(s) 711, 1265
ArsI GACNNNNNNTTYG 2 cut(s) 1202, 1234
AseI ATTAAT 1 cut(s) 1524
Asp700I GAANNNNTTC 2 cut(s) 399, 711
AspLEI GCGC 1 cut(s) 37
AspS9I GGNCC 2 cut(s) 74, 889
AsuHPI GGTGA 2 cut(s) 284, 1406
AvaII GGWCC 2 cut(s) 74, 889
AxyI CCTNAGG 2 cut(s) 380, 971
BaeI ACNNNNGTAYC 2 cut(s) 180, 213
BalI TGGCCA 1 cut(s) 96
BamHI GGATCC 1 cut(s) 877
BanII GRGCYC 1 cut(s) 226
BbsI GAAGAC 2 cut(s) 1293, 1469
Bbv12I GWGCWC 1 cut(s) 226
BbvI GCAGC 6 cut(s) 256, 259, 982, 1081, 1136, 1471
BccI CCATC 5 cut(s) 190, 419, 805, 1184, 1296
BciT130I CCWGG 3 cut(s) 342, 618, 750
BclI TGATCA 2 cut(s) 142, 1497
BcoDI GTCTC 1 cut(s) 723
BfaI CTAG 1 cut(s) 276
BfoI RGCGCY 1 cut(s) 38
BfrI CTTAAG 2 cut(s) 22, 371
BisI GCNGC 6 cut(s) 270, 273, 996, 1095, 1125, 1485
BlsI GCNGC 6 cut(s) 271, 274, 997, 1096, 1126, 1486
Bme1390I CCNGG 3 cut(s) 342, 618, 750
Bme18I GGWCC 2 cut(s) 74, 889
BmeRI GACNNNNNGTC 1 cut(s) 418
BmgT120I GGNCC 2 cut(s) 74, 889
BmiI GGNNCC 2 cut(s) 75, 879
BmrFI CCNGG 3 cut(s) 342, 618, 750
BmsI GCATC 2 cut(s) 828, 1605
BoxI GACNNNNGTC 1 cut(s) 944
BpiI GAAGAC 2 cut(s) 1293, 1469
BpmI CTGGAG 2 cut(s) 951, 1299
BpuEI CTTGAG 2 cut(s) 114, 332
BsaBI GATNNNNATC 1 cut(s) 744
BsaJI CCNNGG 2 cut(s) 616, 617
BsaXI ACNNNNNCTCC 2 cut(s) 213, 243
Bsc4I CCNNNNNNNGG 2 cut(s) 749, 1384
Bse118I RCCGGY 1 cut(s) 666
Bse1I ACTGG 2 cut(s) 934, 1257
Bse21I CCTNAGG 2 cut(s) 380, 971
Bse3DI GCAATG 2 cut(s) 906, 1119
Bse8I GATNNNNATC 1 cut(s) 744
BseBI CCWGG 3 cut(s) 342, 618, 750
BseDI CCNNGG 2 cut(s) 616, 617
BseGI GGATG 3 cut(s) 745, 772, 1195
BseJI GATNNNNATC 1 cut(s) 744
BseLI CCNNNNNNNGG 2 cut(s) 749, 1384
BseMI GCAATG 2 cut(s) 906, 1119
BseMII CTCAG 6 cut(s) 56, 371, 1043, 1221, 1331, 1419
BseNI ACTGG 2 cut(s) 934, 1257
BseXI GCAGC 6 cut(s) 256, 259, 982, 1081, 1136, 1471
BseYI CCCAGC 1 cut(s) 676
BsgI GTGCAG 1 cut(s) 981
BshFI GGCC 2 cut(s) 96, 340
BsiHKAI GWGCWC 1 cut(s) 226
BsiSI CCGG 1 cut(s) 667
BslFI GGGAC 4 cut(s) 324, 545, 599, 1319
BslI CCNNNNNNNGG 2 cut(s) 749, 1384
BsmAI GTCTC 1 cut(s) 723
BsmFI GGGAC 4 cut(s) 324, 545, 599, 1319
BsmI GAATGC 1 cut(s) 238
BsnI GGCC 2 cut(s) 96, 340
Bsp1286I GDGCHC 1 cut(s) 226
BspANI GGCC 2 cut(s) 96, 340
BspCNI CTCAG 6 cut(s) 57, 372, 1044, 1222, 1330, 1420
BspLI GGNNCC 2 cut(s) 75, 879
BspPI GGATC 7 cut(s) 171, 360, 629, 657, 734, 872, 885
BspQI GCTCTTC 2 cut(s) 207, 978
BspTI CTTAAG 2 cut(s) 22, 371
BsrDI GCAATG 2 cut(s) 906, 1119
BsrFI RCCGGY 1 cut(s) 666
BsrI ACTGG 2 cut(s) 934, 1257
BssAI RCCGGY 1 cut(s) 666
BssECI CCNNGG 2 cut(s) 616, 617
Bst2UI CCWGG 3 cut(s) 342, 618, 750
Bst4CI ACNGT 5 cut(s) 296, 963, 1245, 1456, 1468
Bst6I CTCTTC 3 cut(s) 207, 428, 978
BstAFI CTTAAG 2 cut(s) 22, 371
BstC8I GCNNGC 5 cut(s) 39, 98, 1092, 1365, 1542
BstDEI CTNAG 8 cut(s) 65, 380, 971, 1052, 1230, 1317, 1406, 1428
BstF5I GGATG 3 cut(s) 745, 772, 1195
BstH2I RGCGCY 1 cut(s) 38
BstHHI GCGC 1 cut(s) 37
BstMAI GTCTC 1 cut(s) 723
BstMWI GCNNNNNNNGC 5 cut(s) 34, 512, 1124, 1484, 1550
BstNI CCWGG 3 cut(s) 342, 618, 750
BstPAI GACNNNNGTC 1 cut(s) 944
BstSCI CCNGG 3 cut(s) 340, 616, 748
BstV1I GCAGC 6 cut(s) 256, 259, 982, 1081, 1136, 1471
BstV2I GAAGAC 2 cut(s) 1293, 1469
BstX2I RGATCY 2 cut(s) 352, 877
BstYI RGATCY 2 cut(s) 352, 877
Bsu36I CCTNAGG 2 cut(s) 380, 971
BsuRI GGCC 2 cut(s) 96, 340
BtgZI GCGATG 1 cut(s) 1315
BtsCI GGATG 3 cut(s) 745, 772, 1195
BtsIMutI CAGTG 4 cut(s) 1049, 1188, 1227, 1250
Cac8I GCNNGC 5 cut(s) 39, 98, 1092, 1365, 1542
CaiI CAGNNNCTG 3 cut(s) 598, 676, 1646
CfoI GCGC 1 cut(s) 37
Cfr10I RCCGGY 1 cut(s) 666
Cfr13I GGNCC 2 cut(s) 74, 889
CseI GACGC 1 cut(s) 977
Csp6I GTAC 3 cut(s) 332, 572, 685
CspCI CAANNNNNGTGG 2 cut(s) 97, 132
CviAII CATG 2 cut(s) 722, 734
CviQI GTAC 3 cut(s) 332, 572, 685
DdeI CTNAG 8 cut(s) 65, 380, 971, 1052, 1230, 1317, 1406, 1428
DraI TTTAAA 1 cut(s) 1042
DriI GACNNNNNGTC 1 cut(s) 418
EaeI YGGCCR 1 cut(s) 94
Eam1104I CTCTTC 3 cut(s) 207, 428, 978
Eam1105I GACNNNNNGTC 1 cut(s) 418
EarI CTCTTC 3 cut(s) 207, 428, 978
Ecl136II GAGCTC 1 cut(s) 224
Eco147I AGGCCT 1 cut(s) 340
Eco24I GRGCYC 1 cut(s) 226
Eco32I GATATC 1 cut(s) 1225
Eco47I GGWCC 2 cut(s) 74, 889
Eco53kI GAGCTC 1 cut(s) 224
Eco57I CTGAAG 5 cut(s) 143, 386, 878, 1123, 1421
Eco81I CCTNAGG 2 cut(s) 380, 971
EcoICRI GAGCTC 1 cut(s) 224
EcoO109I RGGNCCY 1 cut(s) 889
EcoRII CCWGG 3 cut(s) 340, 616, 748
EcoRV GATATC 1 cut(s) 1225
EcoT38I GRGCYC 1 cut(s) 226
FaeI CATG 2 cut(s) 725, 737
FalI AAGNNNNNCTT 5 cut(s) 37, 201, 233, 975, 1007
FaqI GGGAC 4 cut(s) 324, 545, 599, 1319
FatI CATG 2 cut(s) 721, 733
FbaI TGATCA 2 cut(s) 142, 1497
Fnu4HI GCNGC 6 cut(s) 270, 273, 996, 1095, 1125, 1485
FokI GGATG 3 cut(s) 732, 779, 1202
FriOI GRGCYC 1 cut(s) 226
Fsp4HI GCNGC 6 cut(s) 270, 273, 996, 1095, 1125, 1485
FspBI CTAG 1 cut(s) 276
GlaI GCGC 1 cut(s) 36
GluI GCNGC 6 cut(s) 270, 273, 996, 1095, 1125, 1485
GsaI CCCAGC 1 cut(s) 680
GsuI CTGGAG 2 cut(s) 951, 1299
HaeII RGCGCY 1 cut(s) 38
HaeIII GGCC 2 cut(s) 96, 340
HapII CCGG 1 cut(s) 667
HgaI GACGC 1 cut(s) 977
HhaI GCGC 1 cut(s) 37
Hin1II CATG 2 cut(s) 725, 737
Hin6I GCGC 1 cut(s) 35
HinP1I GCGC 1 cut(s) 35
HincII GTYRAC 2 cut(s) 118, 664
HindII GTYRAC 2 cut(s) 118, 664
HindIII AAGCTT 3 cut(s) 642, 1361, 1403
HinfI GANTC 6 cut(s) 345, 695, 904, 1143, 1194, 1424
HpaII CCGG 1 cut(s) 667
HphI GGTGA 2 cut(s) 284, 1406
Hpy166II GTNNAC 3 cut(s) 118, 292, 664
Hpy188III TCNNGA 9 cut(s) 131, 161, 482, 538, 881, 1198, 1208, 1271, 1459
Hpy8I GTNNAC 3 cut(s) 118, 292, 664
HpyAV CCTTC 7 cut(s) 301, 833, 868, 902, 1075, 1147, 1195
HpyCH4III ACNGT 5 cut(s) 296, 963, 1245, 1456, 1468
HpyCH4V TGCA 8 cut(s) 299, 392, 527, 998, 1097, 1124, 1250, 1568
HpyF10VI GCNNNNNNNGC 5 cut(s) 34, 512, 1124, 1484, 1550
HpyF3I CTNAG 8 cut(s) 65, 380, 971, 1052, 1230, 1317, 1406, 1428
Hsp92II CATG 2 cut(s) 725, 737
HspAI GCGC 1 cut(s) 35
Ksp22I TGATCA 2 cut(s) 142, 1497
LguI GCTCTTC 2 cut(s) 207, 978
LmnI GCTCC 4 cut(s) 221, 229, 1387, 1627
Lsp1109I GCAGC 6 cut(s) 256, 259, 982, 1081, 1136, 1471
LweI GCATC 2 cut(s) 828, 1605
MaeI CTAG 1 cut(s) 276
MaeIII GTNAC 1 cut(s) 530
MfeI CAATTG 3 cut(s) 201, 653, 1098
MflI RGATCY 2 cut(s) 352, 877
MhlI GDGCHC 1 cut(s) 226
MlsI TGGCCA 1 cut(s) 96
MluNI TGGCCA 1 cut(s) 96
MlyI GAGTC 1 cut(s) 339
MmeI TCCRAC 3 cut(s) 101, 1167, 1450
MnlI CCTC 9 cut(s) 183, 375, 429, 666, 753, 1054, 1224, 1425, 1514
Mox20I TGGCCA 1 cut(s) 96
MroXI GAANNNNTTC 2 cut(s) 399, 711
MscI TGGCCA 1 cut(s) 96
MseI TTAA 6 cut(s) 23, 327, 372, 1041, 1524, 1651
MslI CAYNNNNRTG 1 cut(s) 1535
Msp20I TGGCCA 1 cut(s) 96
MspA1I CMGCKG 2 cut(s) 194, 944
MspCI CTTAAG 2 cut(s) 22, 371
MspI CCGG 1 cut(s) 667
MspR9I CCNGG 3 cut(s) 342, 618, 750
MunI CAATTG 3 cut(s) 201, 653, 1098
Mva1269I GAATGC 1 cut(s) 238
MvaI CCWGG 3 cut(s) 342, 618, 750
MwoI GCNNNNNNNGC 5 cut(s) 34, 512, 1124, 1484, 1550
NlaIII CATG 2 cut(s) 725, 737
NlaIV GGNNCC 2 cut(s) 75, 879
PasI CCCWGGG 1 cut(s) 617
PceI AGGCCT 1 cut(s) 340
PciSI GCTCTTC 2 cut(s) 207, 978
PctI GAATGC 1 cut(s) 238
PdmI GAANNNNTTC 2 cut(s) 399, 711
PfeI GAWTC 5 cut(s) 695, 904, 1143, 1194, 1424
PflMI CCANNNNNTGG 1 cut(s) 1384
PfoI TCCNGGA 1 cut(s) 748
PkrI GCNGC 6 cut(s) 271, 274, 997, 1096, 1126, 1486
PleI GAGTC 1 cut(s) 339
PpsI GAGTC 1 cut(s) 339
PpuMI RGGWCCY 1 cut(s) 889
PshAI GACNNNNGTC 1 cut(s) 944
PshBI ATTAAT 1 cut(s) 1524
PsiI TTATAA 1 cut(s) 1329
Psp124BI GAGCTC 1 cut(s) 226
Psp5II RGGWCCY 1 cut(s) 889
Psp6I CCWGG 3 cut(s) 340, 616, 748
PspFI CCCAGC 1 cut(s) 676
PspGI CCWGG 3 cut(s) 340, 616, 748
PspN4I GGNNCC 2 cut(s) 75, 879
PspPI GGNCC 2 cut(s) 74, 889
PspPPI RGGWCCY 1 cut(s) 889
PstNI CAGNNNCTG 3 cut(s) 598, 676, 1646
PsuI RGATCY 2 cut(s) 352, 877
PvuII CAGCTG 2 cut(s) 194, 944
RsaI GTAC 3 cut(s) 333, 573, 686
RsaNI GTAC 3 cut(s) 332, 572, 685
RseI CAYNNNNRTG 1 cut(s) 1535
SacI GAGCTC 1 cut(s) 226
SapI GCTCTTC 2 cut(s) 207, 978
SaqAI TTAA 6 cut(s) 23, 327, 372, 1041, 1524, 1651
SatI GCNGC 6 cut(s) 270, 273, 996, 1095, 1125, 1485
Sau96I GGNCC 2 cut(s) 74, 889
SchI GAGTC 1 cut(s) 339
ScrFI CCNGG 3 cut(s) 342, 618, 750
SduI GDGCHC 1 cut(s) 226
SfaNI GCATC 2 cut(s) 828, 1605
SinI GGWCC 2 cut(s) 74, 889
SmiMI CAYNNNNRTG 1 cut(s) 1535
SmlI CTYRAG 4 cut(s) 22, 129, 347, 371
SmoI CTYRAG 4 cut(s) 22, 129, 347, 371
SseBI AGGCCT 1 cut(s) 340
SspI AATATT 3 cut(s) 61, 364, 757
SspMI CTAG 1 cut(s) 276
SstI GAGCTC 1 cut(s) 226
StuI AGGCCT 1 cut(s) 340
StyD4I CCNGG 3 cut(s) 340, 616, 748
TaaI ACNGT 5 cut(s) 296, 963, 1245, 1456, 1468
TaqI TCGA 2 cut(s) 1216, 1534
TatI WGTACW 1 cut(s) 331
TfiI GAWTC 5 cut(s) 695, 904, 1143, 1194, 1424
Tru1I TTAA 6 cut(s) 23, 327, 372, 1041, 1524, 1651
Tru9I TTAA 6 cut(s) 23, 327, 372, 1041, 1524, 1651
TscAI CASTG 4 cut(s) 1056, 1188, 1234, 1257
TseI GCWGC 6 cut(s) 269, 272, 995, 1094, 1124, 1484
TspDTI ATGAA 4 cut(s) 73, 273, 1088, 1635
TspRI CASTG 4 cut(s) 1056, 1188, 1234, 1257
Van91I CCANNNNNTGG 1 cut(s) 1384
Vha464I CTTAAG 2 cut(s) 22, 371
VpaK11BI GGWCC 2 cut(s) 74, 889
VspI ATTAAT 1 cut(s) 1524
XapI RAATTY 2 cut(s) 711, 1265
XcmI CCANNNNNNNNNTGG 1 cut(s) 1466
XmnI GAANNNNTTC 2 cut(s) 399, 711
XspI CTAG 1 cut(s) 276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.