pycom15g31060

Iaa-amino acid hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0010157
Physical Location & Seq
Reverse (-)
29254764 .. 29260439
5676 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1707 bp
ATGAGTCTCATCGAAAACCCATCCCGAGAGTGCTGGTCCCCACAATTGGCGTTCGAATTTCGACGCCACACGCCCTTCTCATATTCCGAACGTACTGAAAATCTCTTCTTCTATTACCGTCTTTTATCCCCACGTGCTCAAATTTCCCCATCCCATTACTTCTACTCCCCTCATCTCTCTCTCTCTCTCATAATTCCCACACAACTCCATCCCCAAACCCTCTCTCTCTCTCTCTTTCTCAAAATGAATTTTAATCAAACCAAAACAAAACTAATCTACACAAATTCATTATTAATAATCTCCATCTTCGTCCTCGGTTCGATAATTTCACCCGCCGAGACGACGCCGTCCGGCGATCACAGCCACCCGTTCTTCGAGCTCGATGACTGTTGCCCCAGCTGCAAGGCAAGCCCCGAAGCTCGAAAGAATCTGAGCCTCCAAGCGCTAACCTCTCCGCCAACATCACCGCCGTCGTCGTCTCCTCAGAGCTGCGAGGTTTGGACGGAGGCGTGCTCGGTAGCGGTTTTGAGCTTGGCGAAGCGACCGGAGAACGTGGAGTGGTTGAAGACGGTGAGGCGGAGGATCCACGAACATCCAGAGCTCGCTTTCGAGGAGTTCGAGACGAGCCGGCTTGTTCGGGATGAGTTGGACCGGTTGGATATCGGTTACCGGTATCCTTTGGCTAAAACCGGAATCCGAGCTTGGATCGGAACCGGCGGTCCGCCATTTGTCGCCGTCAGGGCTGACATGGACGCCCTTCCAATTCAGGAAGCTGTTGAATGGGAGCACAAGAGCAAAGTAGCCGGCAAAATGCACGCATGTGGGCACGATGCGCATGTGACTATGCTAATTGGCGCTGCCAAGATCCTGAAGAACCGTGAACACCTTCTGAAGGGAACTGTAATATTGCTGTTTCAGCCAGCGGAGGAAGCCGGTAATGGTGCAAAGCAAATGATCGGAGATGATGCTTTGGAGGATGTGGAGGCCATATTTGCAGCTCATGTATCCCATGAGCACCCTACTGGCGTCATCGGGTCAAGGCCTGGTCCTTTGCTAGCTGGCTGTGGCTTCTTCAGAGCTGTTATCAGTGGAACCACCGGCTCTACTGACCCTGTTTTGGCGGCCGCCACGACCGTCATCAGCTTGCAGGGCATCGTATCCCGTGAATCAAACCCTTTGGACTCGCAGGTTGTTTCCGTAACTGCATTCAATGGAGGCGATGATCTTGGGATGATACCCAACACTGTGGTACTTGGTGGTACTTTCAGGGCTTTCTCCAACACAAACTTCTACAGAATTCTTCAAAGAATTGAAGAGGTAATTGTGGAGCAAGCAAGTGTTTACAGATGCTCAGCAACAGTGGACTTCTTCAAAAACCAGAACACCATCTATCCTCCAACAGTGAACGATGAGAAAATGCATGAGCATGTGAGCAAAGTGGCCATCGATTTGCTGGGCCCCACCAACTTCAGGGTGGTGCCACCCATGATGGGGGCGGAGGACTTCTCGTTCTACTCGGAGGTCATCCCTGCTGGCTTCTTCTACATCGGGATCAGGAACGAGACCTTGGGGTCCACACACACGGGCCACTCTCCTTATTTTTTCATCGACGAAGATGTTTTGCCAATTGGGGCTGCAACTCATGCGACAATTGCAGAAAGGTACCTCAACGAGCGGTCGCGGGTGACCACAGACGGGTTATTTTAA

Protein Analysis

569

Amino Acids

62.83

Weight (kDa)

5.84

Isoelectric Point (pI)

47.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M20 PF01546 245 - 553 6.8e-44 Peptidase family M20/M25/M40
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 346, 1570
Acc16I TGCGCA 1 cut(s) 834
Acc36I ACCTGC 1 cut(s) 1177
Acc65I GGTACC 1 cut(s) 1662
AccB1I GGYRCC 2 cut(s) 1477, 1662
AccBSI CCGCTC 1 cut(s) 1675
AccII CGCG 1 cut(s) 1681
AclWI GGATC 5 cut(s) 577, 590, 713, 859, 1559
AcoI YGGCCR 2 cut(s) 1122, 1440
AcsI RAATTY 5 cut(s) 56, 141, 247, 283, 1296
AcuI CTGAAG 4 cut(s) 890, 911, 1057, 1453
AcvI CACGTG 1 cut(s) 134
AcyI GRCGYC 4 cut(s) 64, 344, 753, 1026
AfaI GTAC 4 cut(s) 94, 1251, 1261, 1664
AfeI AGCGCT 1 cut(s) 444
AfiI CCNNNNNNNGG 7 cut(s) 46, 892, 1117, 1470, 1490, 1491, 1695
AgeI ACCGGT 2 cut(s) 651, 669
AgsI TTSAA 6 cut(s) 565, 779, 1210, 1304, 1313, 1372
AjnI CCWGG 1 cut(s) 1042
AjuI GAANNNNNNNTTGG 6 cut(s) 685, 717, 1271, 1303, 1550, 1582
AleI CACNNNNGTG 1 cut(s) 819
AloI GAACNNNNNNTCC 4 cut(s) 703, 735, 1493, 1525
Alw21I GWGCWC 6 cut(s) 139, 381, 515, 603, 789, 1017
Alw26I GTCTC 5 cut(s) 11, 332, 483, 614, 1556
AlwI GGATC 5 cut(s) 577, 590, 713, 859, 1559
Ama87I CYCGRG 1 cut(s) 24
Aor51HI AGCGCT 1 cut(s) 444
AoxI GGCC 6 cut(s) 984, 1040, 1122, 1440, 1456, 1585
ApaI GGGCCC 1 cut(s) 1460
ApeKI GCWGC 5 cut(s) 399, 489, 857, 995, 1634
ApoI RAATTY 5 cut(s) 56, 141, 247, 283, 1296
AseI ATTAAT 1 cut(s) 293
AsiGI ACCGGT 2 cut(s) 651, 669
Asp700I GAANNNNTTC 1 cut(s) 885
Asp718I GGTACC 1 cut(s) 1662
AspLEI GCGC 3 cut(s) 445, 835, 857
AspS9I GGNCC 8 cut(s) 36, 649, 719, 1046, 1456, 1457, 1572, 1585
AsuHPI GGTGA 4 cut(s) 321, 456, 583, 1696
AsuII TTCGAA 1 cut(s) 54
AsuNHI GCTAGC 1 cut(s) 1054
AvaI CYCGRG 1 cut(s) 24
AvaII GGWCC 5 cut(s) 36, 649, 719, 1046, 1572
BaeGI GKGCMC 2 cut(s) 828, 1460
BalI TGGCCA 1 cut(s) 1442
BamHI GGATCC 1 cut(s) 582
BanI GGYRCC 2 cut(s) 1477, 1662
BanII GRGCYC 3 cut(s) 381, 603, 1460
BbrPI CACGTG 1 cut(s) 134
BbsI GAAGAC 1 cut(s) 572
Bbv12I GWGCWC 6 cut(s) 139, 381, 515, 603, 789, 1017
BbvI GCAGC 5 cut(s) 386, 476, 844, 1007, 1621
BccI CCATC 7 cut(s) 28, 157, 216, 311, 1394, 1451, 1483
BceAI ACGGC 3 cut(s) 331, 454, 719
BciT130I CCWGG 1 cut(s) 1044
BciVI GTATCC 3 cut(s) 684, 1015, 1168
BcoDI GTCTC 5 cut(s) 11, 332, 483, 614, 1556
BfaI CTAG 1 cut(s) 1055
BfmI CTRYAG 1 cut(s) 1291
BfoI RGCGCY 2 cut(s) 446, 858
BfuAI ACCTGC 1 cut(s) 1177
BfuI GTATCC 3 cut(s) 684, 1015, 1168
BglI GCCNNNNNGGC 1 cut(s) 740
BisI GCNGC 7 cut(s) 400, 490, 858, 996, 1122, 1125, 1635
BlpI GCTNAGC 1 cut(s) 1351
BlsI GCNGC 7 cut(s) 401, 491, 859, 997, 1123, 1126, 1636
Bme1390I CCNGG 1 cut(s) 1044
Bme18I GGWCC 5 cut(s) 36, 649, 719, 1046, 1572
BmeT110I CYCGRG 1 cut(s) 24
BmgT120I GGNCC 8 cut(s) 36, 649, 719, 1046, 1456, 1457, 1572, 1585
BmiI GGNNCC 9 cut(s) 38, 584, 712, 1093, 1458, 1459, 1479, 1573, 1664
BmrFI CCNGG 1 cut(s) 1044
BmsI GCATC 4 cut(s) 820, 955, 1161, 1337
BmtI GCTAGC 1 cut(s) 1058
BpiI GAAGAC 1 cut(s) 572
BplI GAGNNNNNCTC 4 cut(s) 497, 529, 1490, 1522
Bpu1102I GCTNAGC 1 cut(s) 1351
Bpu14I TTCGAA 1 cut(s) 54
Bsa29I ATCGAT 1 cut(s) 1446
BsaAI YACGTR 1 cut(s) 134
BsaHI GRCGYC 4 cut(s) 64, 344, 753, 1026
BsaI GGTCTC 1 cut(s) 1556
BsaJI CCNNGG 2 cut(s) 313, 1566
BsaWI WCCGGW 4 cut(s) 544, 651, 669, 689
BsaXI ACNNNNNCTCC 2 cut(s) 149, 179
Bsc4I CCNNNNNNNGG 7 cut(s) 46, 892, 1117, 1470, 1490, 1491, 1695
Bse118I RCCGGY 7 cut(s) 627, 651, 669, 713, 803, 932, 1097
Bse1I ACTGG 1 cut(s) 1027
BseBI CCWGG 1 cut(s) 1044
BseCI ATCGAT 1 cut(s) 1446
BseDI CCNNGG 2 cut(s) 313, 1566
BseGI GGATG 8 cut(s) 20, 149, 208, 592, 646, 982, 1236, 1524
BseLI CCNNNNNNNGG 7 cut(s) 46, 892, 1117, 1470, 1490, 1491, 1695
BseMII CTCAG 3 cut(s) 422, 497, 1365
BseNI ACTGG 1 cut(s) 1027
BseRI GAGGAG 2 cut(s) 471, 626
BseSI GKGCMC 2 cut(s) 828, 1460
BseX3I CGGCCG 1 cut(s) 1122
BseXI GCAGC 5 cut(s) 386, 476, 844, 1007, 1621
BseYI CCCAGC 2 cut(s) 395, 1453
Bsh1236I CGCG 1 cut(s) 1681
Bsh1285I CGRYCG 4 cut(s) 545, 1125, 1134, 1679
BshFI GGCC 6 cut(s) 986, 1042, 1124, 1442, 1458, 1587
BshNI GGYRCC 2 cut(s) 1477, 1662
BshTI ACCGGT 2 cut(s) 651, 669
BshVI ATCGAT 1 cut(s) 1446
BsiEI CGRYCG 4 cut(s) 545, 1125, 1134, 1679
BsiHKAI GWGCWC 6 cut(s) 139, 381, 515, 603, 789, 1017
BsiHKCI CYCGRG 1 cut(s) 24
BslFI GGGAC 1 cut(s) 22
BslI CCNNNNNNNGG 7 cut(s) 46, 892, 1117, 1470, 1490, 1491, 1695
BsmAI GTCTC 5 cut(s) 11, 332, 483, 614, 1556
BsmBI CGTCTC 3 cut(s) 332, 483, 614
BsmFI GGGAC 1 cut(s) 22
BsmI GAATGC 1 cut(s) 1205
BsnI GGCC 6 cut(s) 986, 1042, 1124, 1442, 1458, 1587
Bso31I GGTCTC 1 cut(s) 1556
BsoBI CYCGRG 1 cut(s) 24
Bsp119I TTCGAA 1 cut(s) 54
Bsp120I GGGCCC 1 cut(s) 1456
Bsp1286I GDGCHC 8 cut(s) 139, 381, 515, 603, 789, 828, 1017, 1460
Bsp143I GATC 7 cut(s) 355, 582, 705, 864, 954, 1222, 1551
Bsp1720I GCTNAGC 1 cut(s) 1351
BspANI GGCC 6 cut(s) 986, 1042, 1124, 1442, 1458, 1587
BspCNI CTCAG 3 cut(s) 423, 496, 1364
BspDI ATCGAT 1 cut(s) 1446
BspFNI CGCG 1 cut(s) 1681
BspLI GGNNCC 9 cut(s) 38, 584, 712, 1093, 1458, 1459, 1479, 1573, 1664
BspMI ACCTGC 1 cut(s) 1177
BspOI GCTAGC 1 cut(s) 1058
BspPI GGATC 5 cut(s) 577, 590, 713, 859, 1559
BspT104I TTCGAA 1 cut(s) 54
BspT107I GGYRCC 2 cut(s) 1477, 1662
BspTNI GGTCTC 1 cut(s) 1556
BsrBI CCGCTC 1 cut(s) 1675
BsrFI RCCGGY 7 cut(s) 627, 651, 669, 713, 803, 932, 1097
BsrI ACTGG 1 cut(s) 1027
BssAI RCCGGY 7 cut(s) 627, 651, 669, 713, 803, 932, 1097
BssECI CCNNGG 2 cut(s) 313, 1566
BssMI GATC 7 cut(s) 355, 582, 705, 864, 954, 1222, 1551
BssNI GRCGYC 4 cut(s) 64, 344, 753, 1026
BssT1I CCWWGG 1 cut(s) 1566
Bst2UI CCWGG 1 cut(s) 1044
Bst4CI ACNGT 9 cut(s) 119, 389, 571, 878, 901, 1135, 1246, 1360, 1402
Bst6I CTCTTC 2 cut(s) 110, 1308
BstACI GRCGYC 4 cut(s) 64, 344, 753, 1026
BstAPI GCANNNNNTGC 1 cut(s) 1643
BstBAI YACGTR 1 cut(s) 134
BstBI TTCGAA 1 cut(s) 54
BstDEI CTNAG 3 cut(s) 431, 483, 1351
BstEII GGTNACC 2 cut(s) 665, 1684
BstENI CCTNNNNNAGG 1 cut(s) 890
BstF5I GGATG 8 cut(s) 20, 149, 208, 592, 646, 982, 1236, 1524
BstFNI CGCG 1 cut(s) 1681
BstH2I RGCGCY 2 cut(s) 446, 858
BstHHI GCGC 3 cut(s) 445, 835, 857
BstKTI GATC 7 cut(s) 358, 585, 708, 867, 957, 1225, 1554
BstMAI GTCTC 5 cut(s) 11, 332, 483, 614, 1556
BstMBI GATC 7 cut(s) 355, 582, 705, 864, 954, 1222, 1551
BstMCI CGRYCG 4 cut(s) 545, 1125, 1134, 1679
BstNI CCWGG 1 cut(s) 1044
BstNSI RCATGY 3 cut(s) 822, 839, 1430
BstPI GGTNACC 2 cut(s) 665, 1684
BstSCI CCNGG 1 cut(s) 1042
BstSFI CTRYAG 1 cut(s) 1291
BstSLI GKGCMC 2 cut(s) 828, 1460
BstUI CGCG 1 cut(s) 1681
BstV1I GCAGC 5 cut(s) 386, 476, 844, 1007, 1621
BstV2I GAAGAC 1 cut(s) 572
BstX2I RGATCY 2 cut(s) 582, 864
BstXI CCANNNNNNTGG 1 cut(s) 1246
BstYI RGATCY 2 cut(s) 582, 864
BstZI CGGCCG 1 cut(s) 1122
Bsu15I ATCGAT 1 cut(s) 1446
BsuI GTATCC 3 cut(s) 684, 1015, 1168
BsuRI GGCC 6 cut(s) 986, 1042, 1124, 1442, 1458, 1587
BsuTUI ATCGAT 1 cut(s) 1446
BtgZI GCGATG 1 cut(s) 1233
BtsCI GGATG 8 cut(s) 20, 149, 208, 592, 646, 982, 1236, 1524
BtsIMutI CAGTG 4 cut(s) 1093, 1242, 1365, 1407
BveI ACCTGC 1 cut(s) 1177
CciNI GCGGCCGC 1 cut(s) 1122
CfoI GCGC 3 cut(s) 445, 835, 857
Cfr10I RCCGGY 7 cut(s) 627, 651, 669, 713, 803, 932, 1097
Cfr13I GGNCC 8 cut(s) 36, 649, 719, 1046, 1456, 1457, 1572, 1585
ClaI ATCGAT 1 cut(s) 1446
CpoI CGGWCCG 1 cut(s) 719
CseI GACGC 4 cut(s) 72, 352, 761, 1015
Csp6I GTAC 4 cut(s) 93, 1250, 1260, 1663
CspAI ACCGGT 2 cut(s) 651, 669
CspI CGGWCCG 1 cut(s) 719
CviAII CATG 9 cut(s) 748, 819, 836, 1001, 1010, 1421, 1427, 1486, 1643
CviQI GTAC 4 cut(s) 93, 1250, 1260, 1663
DdeI CTNAG 3 cut(s) 431, 483, 1351
DpnI GATC 7 cut(s) 357, 584, 707, 866, 956, 1224, 1553
DpnII GATC 7 cut(s) 355, 582, 705, 864, 954, 1222, 1551
DrdI GACNNNNNNGTC 2 cut(s) 346, 1570
DseDI GACNNNNNNGTC 2 cut(s) 346, 1570
EaeI YGGCCR 2 cut(s) 1122, 1440
EagI CGGCCG 1 cut(s) 1122
Eam1104I CTCTTC 2 cut(s) 110, 1308
EarI CTCTTC 2 cut(s) 110, 1308
EciI GGCGGA 4 cut(s) 444, 592, 711, 1511
Ecl136II GAGCTC 2 cut(s) 379, 601
EclXI CGGCCG 1 cut(s) 1122
Eco130I CCWWGG 1 cut(s) 1566
Eco147I AGGCCT 1 cut(s) 1042
Eco24I GRGCYC 3 cut(s) 381, 603, 1460
Eco31I GGTCTC 1 cut(s) 1556
Eco32I GATATC 1 cut(s) 661
Eco47I GGWCC 5 cut(s) 36, 649, 719, 1046, 1572
Eco47III AGCGCT 1 cut(s) 444
Eco52I CGGCCG 1 cut(s) 1122
Eco53kI GAGCTC 2 cut(s) 379, 601
Eco57I CTGAAG 4 cut(s) 890, 911, 1057, 1453
Eco72I CACGTG 1 cut(s) 134
Eco88I CYCGRG 1 cut(s) 24
Eco91I GGTNACC 2 cut(s) 665, 1684
EcoICRI GAGCTC 2 cut(s) 379, 601
EcoNI CCTNNNNNAGG 1 cut(s) 890
EcoO109I RGGNCCY 1 cut(s) 1457
EcoO65I GGTNACC 2 cut(s) 665, 1684
EcoRI GAATTC 1 cut(s) 1296
EcoRII CCWGG 1 cut(s) 1042
EcoRV GATATC 1 cut(s) 661
EcoT14I CCWWGG 1 cut(s) 1566
EcoT22I ATGCAT 1 cut(s) 1422
EcoT38I GRGCYC 3 cut(s) 381, 603, 1460
ErhI CCWWGG 1 cut(s) 1566
Esp3I CGTCTC 3 cut(s) 332, 483, 614
FaeI CATG 9 cut(s) 751, 822, 839, 1004, 1013, 1424, 1430, 1489, 1646
FaqI GGGAC 1 cut(s) 22
FatI CATG 9 cut(s) 747, 818, 835, 1000, 1009, 1420, 1426, 1485, 1642
FauI CCCGC 2 cut(s) 340, 1674
Fnu4HI GCNGC 7 cut(s) 400, 490, 858, 996, 1122, 1125, 1635
FokI GGATG 8 cut(s) 7, 136, 195, 579, 653, 989, 1243, 1511
FriOI GRGCYC 3 cut(s) 381, 603, 1460
Fsp4HI GCNGC 7 cut(s) 400, 490, 858, 996, 1122, 1125, 1635
FspAI RTGCGCAY 1 cut(s) 834
FspBI CTAG 1 cut(s) 1055
FspI TGCGCA 1 cut(s) 834
GlaI GCGC 3 cut(s) 444, 834, 856
GluI GCNGC 7 cut(s) 400, 490, 858, 996, 1122, 1125, 1635
GsaI CCCAGC 2 cut(s) 399, 1457
HaeII RGCGCY 2 cut(s) 446, 858
HaeIII GGCC 6 cut(s) 986, 1042, 1124, 1442, 1458, 1587
HgaI GACGC 4 cut(s) 72, 352, 761, 1015
HhaI GCGC 3 cut(s) 445, 835, 857
Hin1I GRCGYC 4 cut(s) 64, 344, 753, 1026
Hin1II CATG 9 cut(s) 751, 822, 839, 1004, 1013, 1424, 1430, 1489, 1646
Hin6I GCGC 3 cut(s) 443, 833, 855
HinP1I GCGC 3 cut(s) 443, 833, 855
HinfI GANTC 5 cut(s) 4, 427, 693, 1166, 1181
HphI GGTGA 4 cut(s) 321, 456, 583, 1696
Hpy166II GTNNAC 5 cut(s) 881, 1342, 1363, 1405, 1575
Hpy188I TCNGA 9 cut(s) 88, 432, 486, 698, 710, 891, 959, 1076, 1519
Hpy188III TCNNGA 8 cut(s) 24, 596, 619, 638, 767, 868, 1549, 1555
Hpy8I GTNNAC 5 cut(s) 881, 1342, 1363, 1405, 1575
Hpy99I CGWCG 5 cut(s) 66, 346, 475, 478, 1613
HpyAV CCTTC 4 cut(s) 85, 767, 886, 896
HpyCH4III ACNGT 9 cut(s) 119, 389, 571, 878, 901, 1135, 1246, 1360, 1402
HpyCH4IV ACGT 3 cut(s) 91, 133, 552
HpyCH4V TGCA 9 cut(s) 402, 814, 944, 995, 1147, 1205, 1420, 1637, 1655
HpyF3I CTNAG 3 cut(s) 431, 483, 1351
HpySE526I ACGT 3 cut(s) 91, 133, 552
Hsp92I GRCGYC 4 cut(s) 64, 344, 753, 1026
Hsp92II CATG 9 cut(s) 751, 822, 839, 1004, 1013, 1424, 1430, 1489, 1646
HspAI GCGC 3 cut(s) 443, 833, 855
KpnI GGTACC 1 cut(s) 1666
KroI GCCGGC 2 cut(s) 627, 803
KroNI GCCGGC 2 cut(s) 629, 805
Kzo9I GATC 7 cut(s) 355, 582, 705, 864, 954, 1222, 1551
LmnI GCTCC 2 cut(s) 784, 1327
Lsp1109I GCAGC 5 cut(s) 386, 476, 844, 1007, 1621
LweI GCATC 4 cut(s) 820, 955, 1161, 1337
MaeI CTAG 1 cut(s) 1055
MaeII ACGT 3 cut(s) 91, 133, 552
MaeIII GTNAC 4 cut(s) 665, 838, 1198, 1684
MalI GATC 7 cut(s) 357, 584, 707, 866, 956, 1224, 1553
MbiI CCGCTC 1 cut(s) 1675
MboI GATC 7 cut(s) 355, 582, 705, 864, 954, 1222, 1551
MfeI CAATTG 3 cut(s) 44, 1626, 1650
MflI RGATCY 2 cut(s) 582, 864
MhlI GDGCHC 8 cut(s) 139, 381, 515, 603, 789, 828, 1017, 1460
MlsI TGGCCA 1 cut(s) 1442
MluNI TGGCCA 1 cut(s) 1442
MlyI GAGTC 2 cut(s) 13, 1175
MmeI TCCRAC 4 cut(s) 627, 636, 1302, 1421
Mox20I TGGCCA 1 cut(s) 1442
Mph1103I ATGCAT 1 cut(s) 1422
MroNI GCCGGC 2 cut(s) 627, 803
MroXI GAANNNNTTC 1 cut(s) 885
MscI TGGCCA 1 cut(s) 1442
MseI TTAA 3 cut(s) 252, 293, 1705
MslI CAYNNNNRTG 2 cut(s) 819, 1425
Msp20I TGGCCA 1 cut(s) 1442
MspA1I CMGCKG 2 cut(s) 399, 923
MspR9I CCNGG 1 cut(s) 1044
MunI CAATTG 3 cut(s) 44, 1626, 1650
Mva1269I GAATGC 1 cut(s) 1205
MvaI CCWGG 1 cut(s) 1044
MvnI CGCG 1 cut(s) 1681
NaeI GCCGGC 2 cut(s) 629, 805
NdeII GATC 7 cut(s) 355, 582, 705, 864, 954, 1222, 1551
NgoMIV GCCGGC 2 cut(s) 627, 803
NheI GCTAGC 1 cut(s) 1054
NlaIII CATG 9 cut(s) 751, 822, 839, 1004, 1013, 1424, 1430, 1489, 1646
NlaIV GGNNCC 9 cut(s) 38, 584, 712, 1093, 1458, 1459, 1479, 1573, 1664
NmeAIII GCCGAG 1 cut(s) 361
NmuCI GTSAC 2 cut(s) 838, 1684
NotI GCGGCCGC 1 cut(s) 1122
NsbI TGCGCA 1 cut(s) 834
NsiI ATGCAT 1 cut(s) 1422
NspI RCATGY 3 cut(s) 822, 839, 1430
NspV TTCGAA 1 cut(s) 54
OliI CACNNNNGTG 1 cut(s) 819
PceI AGGCCT 1 cut(s) 1042
PcsI WCGNNNNNNNCGW 1 cut(s) 615
PctI GAATGC 1 cut(s) 1205
PdiI GCCGGC 2 cut(s) 629, 805
PdmI GAANNNNTTC 1 cut(s) 885
PfeI GAWTC 3 cut(s) 427, 693, 1166
PflFI GACNNNGTC 1 cut(s) 346
PinAI ACCGGT 2 cut(s) 651, 669
PkrI GCNGC 7 cut(s) 401, 491, 859, 997, 1123, 1126, 1636
PleI GAGTC 2 cut(s) 12, 1175
PmaCI CACGTG 1 cut(s) 134
PmlI CACGTG 1 cut(s) 134
PpsI GAGTC 2 cut(s) 12, 1175
Ppu21I YACGTR 1 cut(s) 134
PshBI ATTAAT 1 cut(s) 293
Psp124BI GAGCTC 2 cut(s) 381, 603
Psp6I CCWGG 1 cut(s) 1042
PspCI CACGTG 1 cut(s) 134
PspEI GGTNACC 2 cut(s) 665, 1684
PspFI CCCAGC 2 cut(s) 395, 1453
PspGI CCWGG 1 cut(s) 1042
PspN4I GGNNCC 9 cut(s) 38, 584, 712, 1093, 1458, 1459, 1479, 1573, 1664
PspOMI GGGCCC 1 cut(s) 1456
PspPI GGNCC 8 cut(s) 36, 649, 719, 1046, 1456, 1457, 1572, 1585
PsuI RGATCY 2 cut(s) 582, 864
PsyI GACNNNGTC 1 cut(s) 346
PvuII CAGCTG 1 cut(s) 399
RsaI GTAC 4 cut(s) 94, 1251, 1261, 1664
RsaNI GTAC 4 cut(s) 93, 1250, 1260, 1663
RseI CAYNNNNRTG 2 cut(s) 819, 1425
Rsr2I CGGWCCG 1 cut(s) 719
RsrII CGGWCCG 1 cut(s) 719
SacI GAGCTC 2 cut(s) 381, 603
SaqAI TTAA 3 cut(s) 252, 293, 1705
SatI GCNGC 7 cut(s) 400, 490, 858, 996, 1122, 1125, 1635
Sau3AI GATC 7 cut(s) 355, 582, 705, 864, 954, 1222, 1551
Sau96I GGNCC 8 cut(s) 36, 649, 719, 1046, 1456, 1457, 1572, 1585
SchI GAGTC 2 cut(s) 13, 1175
ScrFI CCNGG 1 cut(s) 1044
SduI GDGCHC 8 cut(s) 139, 381, 515, 603, 789, 828, 1017, 1460
SfaNI GCATC 4 cut(s) 820, 955, 1161, 1337
SfcI CTRYAG 1 cut(s) 1291
SfuI TTCGAA 1 cut(s) 54
SinI GGWCC 5 cut(s) 36, 649, 719, 1046, 1572
SmiMI CAYNNNNRTG 2 cut(s) 819, 1425
SseBI AGGCCT 1 cut(s) 1042
SspI AATATT 1 cut(s) 906
SspMI CTAG 1 cut(s) 1055
SstI GAGCTC 2 cut(s) 381, 603
StuI AGGCCT 1 cut(s) 1042
StyD4I CCNGG 1 cut(s) 1042
StyI CCWWGG 1 cut(s) 1566
TaaI ACNGT 9 cut(s) 119, 389, 571, 878, 901, 1135, 1246, 1360, 1402
TaiI ACGT 3 cut(s) 94, 136, 555
TauI GCSGC 2 cut(s) 1124, 1127
TfiI GAWTC 3 cut(s) 427, 693, 1166
Tru1I TTAA 3 cut(s) 252, 293, 1705
Tru9I TTAA 3 cut(s) 252, 293, 1705
TscAI CASTG 4 cut(s) 1093, 1249, 1365, 1407
TseFI GTSAC 2 cut(s) 838, 1684
TseI GCWGC 5 cut(s) 399, 489, 857, 995, 1634
Tsp45I GTSAC 2 cut(s) 838, 1684
TspDTI ATGAA 3 cut(s) 260, 276, 1594
TspGWI ACGGA 2 cut(s) 518, 1186
TspRI CASTG 4 cut(s) 1093, 1249, 1365, 1407
Tth111I GACNNNGTC 1 cut(s) 346
VpaK11BI GGWCC 5 cut(s) 36, 649, 719, 1046, 1572
VspI ATTAAT 1 cut(s) 293
XagI CCTNNNNNAGG 1 cut(s) 890
XapI RAATTY 5 cut(s) 56, 141, 247, 283, 1296
XceI RCATGY 3 cut(s) 822, 839, 1430
XcmI CCANNNNNNNNNTGG 2 cut(s) 1450, 1471
XmnI GAANNNNTTC 1 cut(s) 885
XspI CTAG 1 cut(s) 1055
Zsp2I ATGCAT 1 cut(s) 1422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.