pycom15g31280

Protein of unknown function (DUF740)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0014253
Physical Location & Seq
Reverse (-)
29637548 .. 29639754
2207 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 651 bp
ATGAATGGCTTCAGAGAAGAGAACTCCTTTTGCTATTTCCACCCTCAAGAGTTGGTTGTGGGGGTCTGCTCGCTCTGCCTGACTGAGAGGCTTCTGATCTTGTCTGCCACAAAAGGCCGCCACCACCACCGCCACCATGTTTCCTCCGTCAGAGGCGGCAGTGGCCATAAAAACCCAAATGGCATGCACAAGAAGGCACCCATTCGCCTCCCCAAGATCTTTGCTTTCAGCTCTTTCCTCAATCGGCAGTGGAAACCTGACGACGGCTCAGATCAAGAGGCTTCCACCAGCCAAGAAGACTCATTCATATCAATCAAGTTTGAAGACAATGGAGTAACCTCATGGGAGAAGAACAAAGTCTCTAAGGTCTCTCTAGATCATTGCAACATGTCCTGGAATCACAACTTCAGGAAGGAAGCCAAGGAAGCCAAGGAAACCAAGGAGGCAAGGGATACCAAGAGCGTGATAGAGTACGGGAAATCCCACATCCCGCTTAGGTGGCGGAAGAGGATTGGTCACTTGACCCAGCTCATCAGATGGAAGAGGTCCAACAAGGGCAGTGTGTGCCATGTCAGCAACAAGGTGGAGGGTGTCAAGGTAAGGAAGGGTTGGATAAGAAGCTTGACAAAGAGGAGATGGGCCATGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF740

Protein Analysis

217

Amino Acids

25.06

Weight (kDa)

10.05

Isoelectric Point (pI)

54.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014253)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21830
fragaria_vesca FvH4_2g26860
malus_domestica MD08G1163000.v1.1 MD15G1349700.v1.1
prunus_persica Prupe.1G498100_v2.0.a1
pyrus_communis pycom08g14080 pycom15g31280
rosa_chinensis RchiOBHm_Chr6g0295711
rosa_laevigata RLG00000011773
rosa_multiflora Rmu_sc0007372.1_g000002
rosa_roxburghii Rroxscaffold_7G00172190
rosa_rugosa Rorug06G0253700
rosa_samantha Rh6AG367100 Rh6BG374900 Rh6CG381200 Rh6DG368000
rosa_wichuraiana Rw6G032040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 196
AciI CCGC 5 cut(s) 118, 130, 156, 491, 502
AcoI YGGCCR 1 cut(s) 163
AcuI CTGAAG 1 cut(s) 391
AfaI GTAC 1 cut(s) 473
AfiI CCNNNNNNNGG 1 cut(s) 263
AflIII ACRYGT 1 cut(s) 387
AgsI TTSAA 1 cut(s) 323
AjnI CCWGG 1 cut(s) 392
AluBI AGCT 3 cut(s) 231, 529, 621
AluI AGCT 3 cut(s) 231, 529, 621
Alw26I GTCTC 2 cut(s) 364, 373
AoxI GGCC 3 cut(s) 115, 163, 639
Asp700I GAANNNNTTC 1 cut(s) 8
AspS9I GGNCC 2 cut(s) 546, 639
AvaII GGWCC 1 cut(s) 546
BalI TGGCCA 1 cut(s) 165
BanI GGYRCC 1 cut(s) 196
BbsI GAAGAC 2 cut(s) 303, 330
BccI CCATC 2 cut(s) 531, 630
BceAI ACGGC 1 cut(s) 280
BciT130I CCWGG 1 cut(s) 394
BciVI GTATCC 1 cut(s) 445
BcoDI GTCTC 2 cut(s) 364, 373
BfaI CTAG 1 cut(s) 374
BfuI GTATCC 1 cut(s) 445
BglII AGATCT 1 cut(s) 216
BisI GCNGC 2 cut(s) 118, 157
BlsI GCNGC 2 cut(s) 119, 158
Bme1390I CCNGG 1 cut(s) 394
Bme18I GGWCC 1 cut(s) 546
BmgT120I GGNCC 2 cut(s) 546, 639
BmiI GGNNCC 1 cut(s) 198
BmrFI CCNGG 1 cut(s) 394
BpiI GAAGAC 2 cut(s) 303, 330
Bpu10I CCTNAGC 1 cut(s) 494
BpuEI CTTGAG 1 cut(s) 30
BsaI GGTCTC 1 cut(s) 373
BsaJI CCNNGG 4 cut(s) 420, 429, 438, 642
Bsc4I CCNNNNNNNGG 1 cut(s) 263
Bse3DI GCAATG 1 cut(s) 379
BseBI CCWGG 1 cut(s) 394
BseDI CCNNGG 4 cut(s) 420, 429, 438, 642
BseGI GGATG 1 cut(s) 486
BseLI CCNNNNNNNGG 1 cut(s) 263
BseMI GCAATG 1 cut(s) 379
BseMII CTCAG 2 cut(s) 75, 282
BseRI GAGGAG 1 cut(s) 646
BseYI CCCAGC 1 cut(s) 525
BshFI GGCC 3 cut(s) 117, 165, 641
BshNI GGYRCC 1 cut(s) 196
BslI CCNNNNNNNGG 1 cut(s) 263
BsmAI GTCTC 2 cut(s) 364, 373
BsnI GGCC 3 cut(s) 117, 165, 641
Bso31I GGTCTC 1 cut(s) 373
Bsp143I GATC 4 cut(s) 96, 216, 271, 376
Bsp19I CCATGG 1 cut(s) 642
BspACI CCGC 5 cut(s) 118, 130, 156, 491, 502
BspANI GGCC 3 cut(s) 117, 165, 641
BspCNI CTCAG 2 cut(s) 76, 281
BspLI GGNNCC 1 cut(s) 198
BspT107I GGYRCC 1 cut(s) 196
BspTNI GGTCTC 1 cut(s) 373
BsrDI GCAATG 1 cut(s) 379
BssECI CCNNGG 4 cut(s) 420, 429, 438, 642
BssMI GATC 4 cut(s) 96, 216, 271, 376
BssT1I CCWWGG 4 cut(s) 420, 429, 438, 642
Bst2UI CCWGG 1 cut(s) 394
Bst6I CTCTTC 3 cut(s) 12, 500, 536
BstAPI GCANNNNNTGC 1 cut(s) 564
BstC8I GCNNGC 2 cut(s) 71, 185
BstDEI CTNAG 4 cut(s) 84, 268, 363, 494
BstDSI CCRYGG 1 cut(s) 642
BstF5I GGATG 1 cut(s) 486
BstKTI GATC 4 cut(s) 99, 219, 274, 379
BstMAI GTCTC 2 cut(s) 364, 373
BstMBI GATC 4 cut(s) 96, 216, 271, 376
BstMWI GCNNNNNNNGC 6 cut(s) 75, 162, 425, 499, 564, 573
BstNI CCWGG 1 cut(s) 394
BstNSI RCATGY 2 cut(s) 187, 391
BstSCI CCNGG 1 cut(s) 392
BstV2I GAAGAC 2 cut(s) 303, 330
BstX2I RGATCY 1 cut(s) 216
BstYI RGATCY 1 cut(s) 216
BsuI GTATCC 1 cut(s) 445
BsuRI GGCC 3 cut(s) 117, 165, 641
BtgI CCRYGG 1 cut(s) 642
BtsCI GGATG 1 cut(s) 486
BtsI GCAGTG 3 cut(s) 166, 254, 565
BtsIMutI CAGTG 3 cut(s) 166, 254, 565
Cac8I GCNNGC 2 cut(s) 71, 185
Cfr13I GGNCC 2 cut(s) 546, 639
Csp6I GTAC 1 cut(s) 472
CviAII CATG 6 cut(s) 137, 184, 342, 388, 569, 643
CviQI GTAC 1 cut(s) 472
DdeI CTNAG 4 cut(s) 84, 268, 363, 494
DpnI GATC 4 cut(s) 98, 218, 273, 378
DpnII GATC 4 cut(s) 96, 216, 271, 376
EaeI YGGCCR 1 cut(s) 163
Eam1104I CTCTTC 3 cut(s) 12, 500, 536
EarI CTCTTC 3 cut(s) 12, 500, 536
EciI GGCGGA 1 cut(s) 517
Eco130I CCWWGG 4 cut(s) 420, 429, 438, 642
Eco31I GGTCTC 1 cut(s) 373
Eco47I GGWCC 1 cut(s) 546
Eco57I CTGAAG 1 cut(s) 391
EcoRII CCWGG 1 cut(s) 392
EcoT14I CCWWGG 4 cut(s) 420, 429, 438, 642
ErhI CCWWGG 4 cut(s) 420, 429, 438, 642
FaeI CATG 6 cut(s) 140, 187, 345, 391, 572, 646
FaiI YATR 8 cut(s) 138, 168, 185, 308, 343, 389, 570, 644
FatI CATG 6 cut(s) 136, 183, 341, 387, 568, 642
FauI CCCGC 1 cut(s) 498
Fnu4HI GCNGC 2 cut(s) 118, 157
FokI GGATG 1 cut(s) 473
Fsp4HI GCNGC 2 cut(s) 118, 157
FspBI CTAG 1 cut(s) 374
GluI GCNGC 2 cut(s) 118, 157
GsaI CCCAGC 1 cut(s) 529
HaeIII GGCC 3 cut(s) 117, 165, 641
Hin1II CATG 6 cut(s) 140, 187, 345, 391, 572, 646
HindIII AAGCTT 1 cut(s) 619
HinfI GANTC 2 cut(s) 299, 397
Hpy188I TCNGA 5 cut(s) 14, 96, 152, 271, 536
Hpy188III TCNNGA 4 cut(s) 47, 275, 374, 409
Hpy99I CGWCG 1 cut(s) 266
HpyAV CCTTC 3 cut(s) 187, 406, 598
HpyCH4V TGCA 2 cut(s) 187, 384
HpyF10VI GCNNNNNNNGC 6 cut(s) 75, 162, 425, 499, 564, 573
HpyF3I CTNAG 4 cut(s) 84, 268, 363, 494
Hsp92II CATG 6 cut(s) 140, 187, 345, 391, 572, 646
Kzo9I GATC 4 cut(s) 96, 216, 271, 376
LpnPI CCDG 7 cut(s) 92, 270, 301, 379, 394, 406, 539
MaeI CTAG 1 cut(s) 374
MaeIII GTNAC 2 cut(s) 334, 515
MalI GATC 4 cut(s) 98, 218, 273, 378
MboI GATC 4 cut(s) 96, 216, 271, 376
MboII GAAGA 6 cut(s) 29, 308, 335, 361, 517, 553
MflI RGATCY 1 cut(s) 216
MlsI TGGCCA 1 cut(s) 165
MluNI TGGCCA 1 cut(s) 165
MlyI GAGTC 1 cut(s) 293
MmeI TCCRAC 2 cut(s) 573, 590
Mox20I TGGCCA 1 cut(s) 165
MroXI GAANNNNTTC 1 cut(s) 8
MscI TGGCCA 1 cut(s) 165
Msp20I TGGCCA 1 cut(s) 165
MspR9I CCNGG 1 cut(s) 394
MvaI CCWGG 1 cut(s) 394
MwoI GCNNNNNNNGC 6 cut(s) 75, 162, 425, 499, 564, 573
NcoI CCATGG 1 cut(s) 642
NdeII GATC 4 cut(s) 96, 216, 271, 376
NlaIII CATG 6 cut(s) 140, 187, 345, 391, 572, 646
NlaIV GGNNCC 1 cut(s) 198
NmuCI GTSAC 1 cut(s) 515
NspI RCATGY 2 cut(s) 187, 391
PaeI GCATGC 1 cut(s) 187
PciI ACATGT 1 cut(s) 387
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 1 cut(s) 397
PfoI TCCNGGA 1 cut(s) 392
PkrI GCNGC 2 cut(s) 119, 158
PleI GAGTC 1 cut(s) 293
PpsI GAGTC 1 cut(s) 293
PscI ACATGT 1 cut(s) 387
Psp6I CCWGG 1 cut(s) 392
PspFI CCCAGC 1 cut(s) 525
PspGI CCWGG 1 cut(s) 392
PspN4I GGNNCC 1 cut(s) 198
PspPI GGNCC 2 cut(s) 546, 639
PsuI RGATCY 1 cut(s) 216
RsaI GTAC 1 cut(s) 473
RsaNI GTAC 1 cut(s) 472
SatI GCNGC 2 cut(s) 118, 157
Sau3AI GATC 4 cut(s) 96, 216, 271, 376
Sau96I GGNCC 2 cut(s) 546, 639
SchI GAGTC 1 cut(s) 293
ScrFI CCNGG 1 cut(s) 394
SinI GGWCC 1 cut(s) 546
SmlI CTYRAG 1 cut(s) 45
SmoI CTYRAG 1 cut(s) 45
SphI GCATGC 1 cut(s) 187
SsiI CCGC 5 cut(s) 118, 130, 156, 491, 502
SspMI CTAG 1 cut(s) 374
StyD4I CCNGG 1 cut(s) 392
StyI CCWWGG 4 cut(s) 420, 429, 438, 642
TauI GCSGC 2 cut(s) 120, 159
TfiI GAWTC 1 cut(s) 397
TscAI CASTG 3 cut(s) 166, 254, 565
TseFI GTSAC 1 cut(s) 515
Tsp45I GTSAC 1 cut(s) 515
TspDTI ATGAA 2 cut(s) 17, 295
TspGWI ACGGA 1 cut(s) 136
TspRI CASTG 3 cut(s) 166, 254, 565
VpaK11BI GGWCC 1 cut(s) 546
XbaI TCTAGA 1 cut(s) 373
XceI RCATGY 2 cut(s) 187, 391
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 1 cut(s) 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.