Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0006062

Physical Location & Seq

Genomic Coordinates Forward (+)

30659945 .. 30664410

Gene Structure 4466 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1608 bp

ATGCATCCCTTCTGCTGCATCTCCACGCTCTCCGATCACTCACCAGACCTCCACATGACCTCCTTCGGCTCCAGATCCGACACCCACACCCGCATCGCCAAAATCGACCCGAACAGCGTCAACAATCACAGCTACAGCCACAGCCATTGCAGCGCTGACTACGGACGCCTCAGTCAGCGCACCACGGCGGCTGCTGCTGCTGCCGCCGTGGCAGTCGTGGCTCGGCCACCCTCGGCAAGGGAGCAGCCGGTCGACGTCCAGATAAACGACATCGTCGGGAATGGCGTGTCCGGAATTCTGTACAAGTGGGTGAATTACGGCAAGGGGTGGCGGCCGAGGTGGTTCGTTCTGCAAGACGGAGTTTTGTCGTATTATAAGATTCACGGCCCTGATAGGATCGTCGTTACGGTTGAGACTGAGAAAGGATCAAAAGTCATCGGCGACGAATCGATGCGGCGGATTTCGAGGCATAAGAACGGCATTCCTCACCAGCTCCGCCGCAAGCCTTTCGGCGAAGTCCATCTCAAGGTTTCGAGCGTCCGCGAAAGCAGATCGGATGATAAGAGGTTTTCGATCTTCACAGGCACAAAGAGGTTGCATTTGAGGGCAGAGACTCGAGAAGATCGACACGAATGGGTGGAAGCGTTGCAGGCTGTGAAAGACATGTTCCCGCGGATCTCCAACAGCGAGTTGATGGCTCCGGTCGACAATGTCGCCCTTTCAACTGACAAGGTGAGGCAGCGGCTTGTGGAAGAAGGTGTTAGGGAGGCAGCCATTCAGGACACGGAGCAGATAATGAGGACTGAGTTCGCCGCGTTGCAGAACCACCTCTTGCTGCTCAAGCAGAAGCAGTGGCTCCTTATTGACACGCTGCGACAGTTAGAGACAGAAAAGGTAGATTTGGAGAATACAGTGGTAGATGAGAGCCGACAGTTGAAAGATTCCGGGGATTCTTCTAGATCAAGGCAAGACAAATTCAGTGAAGGGAGTGCAAGTGAATCGGATGAGGATAATGAAAGAGTTGATGCTGCAGAGGAAGAAACAGATGAAGAGGACAATGCTTTCTTTGATACTCGTGATTTTCTATCATCAAGTTCTTTTAAAAGTAATGGGTCCGAGTATCGGATATCATCTGTGTCTTCAGACGATGAAGGGATAAATGCCATTGAACCAGAAGATGGTCTTGATCCATCTATGGGATCTGTTGGAACTAACTTTCCTCTTGTCAAGCGCCGTAATAAGTTGCCGGACCCTGTTGAAAAAGAGAAGAGTGTTAGTCTTTGGTCAATGATTAAGGATAACATTGGGAAGGATCTCACAAAAATATGTCTTCCTGTTTACTTTAATGAGCCTCTATCTACTCTACAAAAGGGTTTTGAGGATTTGGAATACTCGTATCTCCTCGATCGAGCATATGAATGGGGTAAAAGGGGTAATAGCCTTATGAGGATACTTTATGTGGCTGCTTTTGCTGTGTCTGGATATGCATCAACTGAAGGAAGAATTTGCAAGCCATTCAACCCATTATTAGGGGAAACATACGAGGCTGACTATCCAGACAAAGGCCTCCGATTCTTCTCAGAGAAGGTGTTAGTCATCACCCCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Oxysterol_BP PH_11

Protein Analysis

536

Amino Acids

60.45

Weight (kDa)

5.65

Isoelectric Point (pI)

42.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PH	PF00169	97 - 219	1.1e-12	PH domain
PH_11	PF15413	101 - 217	2e-14	Pleckstrin homology domain
Oxysterol_BP	PF01237	426 - 530	5.7e-42	Oxysterol-binding protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006062)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G77730 AT2G31020 AT4G08180 AT4G08180 AT4G08180 AT4G08180
fragaria_vesca	FvH4_2g26250 FvH4_2g26250 FvH4_2g26250
malus_domestica	MD00G1220800.v1.1 MD08G1170000.v1.1 MD15G1357000.v1.1
prunus_persica	Prupe.1G504000_v2.0.a1 Prupe.1G504000_v2.0.a1
pyrus_communis	pycom08g14550 pycom15g31910
rosa_chinensis	RchiOBHm_Chr6g0294861
rosa_laevigata	RLG00000011836
rosa_multiflora	Rmu_co8074688.1_g000001 Rmu_sc0008966.1_g000015
rosa_roxburghii	Rroxscaffold_7G00172950
rosa_rugosa	Rorug06G0247200
rosa_samantha	Rh6AG360900 Rh6BG367500 Rh6CG374700 Rh6DG361400
rosa_wichuraiana	Rw6G031480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	375
AasI	GACNNNNNNGTC	1 cut(s)	171
AatII	GACGTC	1 cut(s)	258
AccB7I	CCANNNNNTGG	1 cut(s)	1178
AccI	GTMKAC	2 cut(s)	252, 705
AccII	CGCG	3 cut(s)	543, 673, 815
AccIII	TCCGGA	1 cut(s)	290
AclWI	GGATC	7 cut(s)	69, 404, 433, 683, 1181, 1207, 1320
AcoI	YGGCCR	2 cut(s)	224, 332
AcsI	RAATTY	3 cut(s)	294, 974, 1503
AcuI	CTGAAG	2 cut(s)	1125, 1515
AcyI	GRCGYC	2 cut(s)	166, 255
AfaI	GTAC	1 cut(s)	302
AfeI	AGCGCT	1 cut(s)	154
AfiI	CCNNNNNNNGG	7 cut(s)	237, 526, 1122, 1178, 1196, 1529, 1562
AflIII	ACRYGT	1 cut(s)	663
AgsI	TTSAA	5 cut(s)	723, 937, 1169, 1259, 1519
AloI	GAACNNNNNNTCC	2 cut(s)	1201, 1233
AluBI	AGCT	2 cut(s)	132, 493
AluI	AGCT	2 cut(s)	132, 493
Alw26I	GTCTC	3 cut(s)	407, 605, 878
AlwI	GGATC	7 cut(s)	69, 404, 433, 683, 1181, 1207, 1320
Ama87I	CYCGRG	1 cut(s)	615
Aor13HI	TCCGGA	1 cut(s)	290
Aor51HI	AGCGCT	1 cut(s)	154
AoxI	GGCC	4 cut(s)	224, 332, 385, 1564
ApoI	RAATTY	3 cut(s)	294, 974, 1503
AspLEI	GCGC	3 cut(s)	155, 180, 1233
AspS9I	GGNCC	3 cut(s)	386, 1113, 1249
AsuC2I	CCSGG	1 cut(s)	946
AsuHPI	GGTGA	5 cut(s)	33, 322, 479, 745, 1591
AvaI	CYCGRG	1 cut(s)	615
AvaII	GGWCC	2 cut(s)	1113, 1249
BauI	CACGAG	1 cut(s)	1074
BbsI	GAAGAC	2 cut(s)	1131, 1322
BccI	CCATC	4 cut(s)	528, 688, 1172, 1198
BceAI	ACGGC	6 cut(s)	191, 201, 334, 400, 493, 1218
BcgI	CGANNNNNNTGC	4 cut(s)	490, 524, 981, 1015
BciVI	GTATCC	1 cut(s)	1443
BcnI	CCSGG	1 cut(s)	946
BcoDI	GTCTC	3 cut(s)	407, 605, 878
BfaI	CTAG	1 cut(s)	957
BfmI	CTRYAG	2 cut(s)	133, 1029
BfoI	RGCGCY	2 cut(s)	156, 1234
BfuI	GTATCC	1 cut(s)	1443
BglI	GCCNNNNNGGC	1 cut(s)	209
Bme1390I	CCNGG	1 cut(s)	946
Bme18I	GGWCC	2 cut(s)	1113, 1249
BmeT110I	CYCGRG	1 cut(s)	615
BmgT120I	GGNCC	3 cut(s)	386, 1113, 1249
BmiI	GGNNCC	5 cut(s)	70, 699, 857, 1114, 1251
BmrFI	CCNGG	1 cut(s)	946
BmsI	GCATC	6 cut(s)	13, 27, 102, 441, 1015, 1496
BpiI	GAAGAC	2 cut(s)	1131, 1322
BpmI	CTGGAG	1 cut(s)	55
BpuEI	CTTGAG	2 cut(s)	509, 824
BpuMI	CCSGG	1 cut(s)	946
Bsa29I	ATCGAT	1 cut(s)	449
BsaBI	GATNNNNATC	1 cut(s)	1486
BsaHI	GRCGYC	2 cut(s)	166, 255
BsaJI	CCNNGG	6 cut(s)	183, 207, 231, 335, 671, 945
BsaWI	WCCGGW	2 cut(s)	290, 700
BsaXI	ACNNNNNCTCC	6 cut(s)	33, 44, 63, 74, 979, 1009
Bsc4I	CCNNNNNNNGG	7 cut(s)	237, 526, 1122, 1178, 1196, 1529, 1562
Bse118I	RCCGGY	1 cut(s)	247
Bse3DI	GCAATG	1 cut(s)	145
Bse8I	GATNNNNATC	1 cut(s)	1486
BseAI	TCCGGA	1 cut(s)	290
BseCI	ATCGAT	1 cut(s)	449
BseDI	CCNNGG	6 cut(s)	183, 207, 231, 335, 671, 945
BseGI	GGATG	3 cut(s)	4, 562, 1009
BseJI	GATNNNNATC	1 cut(s)	1486
BseLI	CCNNNNNNNGG	7 cut(s)	237, 526, 1122, 1178, 1196, 1529, 1562
BseMI	GCAATG	1 cut(s)	145
BseMII	CTCAG	4 cut(s)	184, 408, 795, 1593
BseRI	GAGGAG	1 cut(s)	1391
BseX3I	CGGCCG	1 cut(s)	332
Bsh1236I	CGCG	3 cut(s)	543, 673, 815
Bsh1285I	CGRYCG	4 cut(s)	252, 335, 705, 1408
BshFI	GGCC	4 cut(s)	226, 334, 387, 1566
BshVI	ATCGAT	1 cut(s)	449
BsiEI	CGRYCG	4 cut(s)	252, 335, 705, 1408
BsiHKCI	CYCGRG	1 cut(s)	615
BsiSI	CCGG	5 cut(s)	248, 291, 701, 945, 1247
BslI	CCNNNNNNNGG	7 cut(s)	237, 526, 1122, 1178, 1196, 1529, 1562
BsmAI	GTCTC	3 cut(s)	407, 605, 878
BsmI	GAATGC	1 cut(s)	480
BsnI	GGCC	4 cut(s)	226, 334, 387, 1566
BsoBI	CYCGRG	1 cut(s)	615
Bsp13I	TCCGGA	1 cut(s)	290
Bsp1407I	TGTACA	1 cut(s)	300
BspANI	GGCC	4 cut(s)	226, 334, 387, 1566
BspCNI	CTCAG	4 cut(s)	183, 409, 796, 1592
BspDI	ATCGAT	1 cut(s)	449
BspEI	TCCGGA	1 cut(s)	290
BspFNI	CGCG	3 cut(s)	543, 673, 815
BspLI	GGNNCC	5 cut(s)	70, 699, 857, 1114, 1251
BspMAI	CTGCAG	1 cut(s)	1033
BspPI	GGATC	7 cut(s)	69, 404, 433, 683, 1181, 1207, 1320
BsrDI	GCAATG	1 cut(s)	145
BsrFI	RCCGGY	1 cut(s)	247
BsrGI	TGTACA	1 cut(s)	300
BssAI	RCCGGY	1 cut(s)	247
BssECI	CCNNGG	6 cut(s)	183, 207, 231, 335, 671, 945
BssNI	GRCGYC	2 cut(s)	166, 255
BssSI	CACGAG	1 cut(s)	1074
Bst2BI	CACGAG	1 cut(s)	1074
Bst4CI	ACNGT	4 cut(s)	409, 879, 913, 933
Bst6I	CTCTTC	2 cut(s)	1044, 1262
BstACI	GRCGYC	2 cut(s)	166, 255
BstAUI	TGTACA	1 cut(s)	300
BstC8I	GCNNGC	3 cut(s)	503, 651, 1511
BstDEI	CTNAG	4 cut(s)	170, 417, 804, 1579
BstDSI	CCRYGG	3 cut(s)	183, 207, 671
BstENI	CCTNNNNNAGG	1 cut(s)	235
BstF5I	GGATG	3 cut(s)	4, 562, 1009
BstFNI	CGCG	3 cut(s)	543, 673, 815
BstH2I	RGCGCY	2 cut(s)	156, 1234
BstHHI	GCGC	3 cut(s)	155, 180, 1233
BstMAI	GTCTC	3 cut(s)	407, 605, 878
BstMCI	CGRYCG	4 cut(s)	252, 335, 705, 1408
BstNSI	RCATGY	1 cut(s)	667
BstSCI	CCNGG	1 cut(s)	944
BstSFI	CTRYAG	2 cut(s)	133, 1029
BstUI	CGCG	3 cut(s)	543, 673, 815
BstV2I	GAAGAC	2 cut(s)	1131, 1322
BstX2I	RGATCY	4 cut(s)	74, 675, 1199, 1312
BstYI	RGATCY	4 cut(s)	74, 675, 1199, 1312
BstZI	CGGCCG	1 cut(s)	332
Bsu15I	ATCGAT	1 cut(s)	449
BsuI	GTATCC	1 cut(s)	1443
BsuRI	GGCC	4 cut(s)	226, 334, 387, 1566
BsuTUI	ATCGAT	1 cut(s)	449
BtgI	CCRYGG	3 cut(s)	183, 207, 671
BtgZI	GCGATG	1 cut(s)	79
BtsCI	GGATG	3 cut(s)	4, 562, 1009
BtsI	GCAGTG	1 cut(s)	857
BtsIMutI	CAGTG	3 cut(s)	857, 918, 985
Cac8I	GCNNGC	3 cut(s)	503, 651, 1511
CfoI	GCGC	3 cut(s)	155, 180, 1233
Cfr10I	RCCGGY	1 cut(s)	247
Cfr13I	GGNCC	3 cut(s)	386, 1113, 1249
Cfr42I	CCGCGG	1 cut(s)	674
ClaI	ATCGAT	1 cut(s)	449
CseI	GACGC	3 cut(s)	106, 174, 526
Csp6I	GTAC	1 cut(s)	301
CspCI	CAANNNNNGTGG	2 cut(s)	128, 163
CviAII	CATG	3 cut(s)	55, 664, 1605
CviQI	GTAC	1 cut(s)	301
DdeI	CTNAG	4 cut(s)	170, 417, 804, 1579
DraI	TTTAAA	1 cut(s)	1102
DrdI	GACNNNNNNGTC	1 cut(s)	171
DseDI	GACNNNNNNGTC	1 cut(s)	171
EaeI	YGGCCR	2 cut(s)	224, 332
EagI	CGGCCG	1 cut(s)	332
Eam1104I	CTCTTC	2 cut(s)	1044, 1262
EarI	CTCTTC	2 cut(s)	1044, 1262
EciI	GGCGGA	2 cut(s)	472, 485
EclXI	CGGCCG	1 cut(s)	332
Eco147I	AGGCCT	1 cut(s)	1566
Eco32I	GATATC	1 cut(s)	1128
Eco47I	GGWCC	2 cut(s)	1113, 1249
Eco47III	AGCGCT	1 cut(s)	154
Eco52I	CGGCCG	1 cut(s)	332
Eco57I	CTGAAG	2 cut(s)	1125, 1515
Eco88I	CYCGRG	1 cut(s)	615
EcoNI	CCTNNNNNAGG	1 cut(s)	235
EcoRI	GAATTC	1 cut(s)	294
EcoRV	GATATC	1 cut(s)	1128
EcoT22I	ATGCAT	2 cut(s)	6, 1489
FaeI	CATG	3 cut(s)	58, 667, 1608
FalI	AAGNNNNNCTT	2 cut(s)	1167, 1199
FatI	CATG	3 cut(s)	54, 663, 1604
FauI	CCCGC	2 cut(s)	98, 678
FauNDI	CATATG	1 cut(s)	1414
FblI	GTMKAC	2 cut(s)	252, 705
FokI	GGATG	2 cut(s)	569, 1016
FspBI	CTAG	1 cut(s)	957
GlaI	GCGC	3 cut(s)	154, 179, 1232
GsuI	CTGGAG	1 cut(s)	55
HaeII	RGCGCY	2 cut(s)	156, 1234
HaeIII	GGCC	4 cut(s)	226, 334, 387, 1566
HapII	CCGG	5 cut(s)	248, 291, 701, 945, 1247
HgaI	GACGC	3 cut(s)	106, 174, 526
HhaI	GCGC	3 cut(s)	155, 180, 1233
Hin1I	GRCGYC	2 cut(s)	166, 255
Hin1II	CATG	3 cut(s)	58, 667, 1608
Hin6I	GCGC	3 cut(s)	153, 178, 1231
HinP1I	GCGC	3 cut(s)	153, 178, 1231
HincII	GTYRAC	3 cut(s)	121, 253, 706
HindII	GTYRAC	3 cut(s)	121, 253, 706
HinfI	GANTC	7 cut(s)	379, 446, 613, 941, 950, 998, 1572
HpaII	CCGG	5 cut(s)	248, 291, 701, 945, 1247
HphI	GGTGA	5 cut(s)	33, 322, 479, 745, 1591
Hpy166II	GTNNAC	4 cut(s)	121, 253, 706, 1339
Hpy188I	TCNGA	9 cut(s)	34, 79, 556, 1003, 1117, 1125, 1144, 1571, 1582
Hpy8I	GTNNAC	4 cut(s)	121, 253, 706, 1339
Hpy99I	CGWCG	4 cut(s)	257, 278, 404, 446
HpyAV	CCTTC	8 cut(s)	19, 73, 749, 977, 1145, 1303, 1490, 1579
HpyCH4III	ACNGT	4 cut(s)	409, 879, 913, 933
HpyCH4IV	ACGT	1 cut(s)	255
HpyF3I	CTNAG	4 cut(s)	170, 417, 804, 1579
HpySE526I	ACGT	1 cut(s)	255
Hsp92I	GRCGYC	2 cut(s)	166, 255
Hsp92II	CATG	3 cut(s)	58, 667, 1608
HspAI	GCGC	3 cut(s)	153, 178, 1231
Kpn2I	TCCGGA	1 cut(s)	290
KspI	CCGCGG	1 cut(s)	674
LmnI	GCTCC	6 cut(s)	74, 241, 498, 703, 787, 861
LweI	GCATC	6 cut(s)	13, 27, 102, 441, 1015, 1496
MaeI	CTAG	1 cut(s)	957
MaeII	ACGT	1 cut(s)	255
MaeIII	GTNAC	1 cut(s)	403
MflI	RGATCY	4 cut(s)	74, 675, 1199, 1312
MluCI	AATT	4 cut(s)	294, 313, 974, 1503
MlyI	GAGTC	1 cut(s)	607
MmeI	TCCRAC	3 cut(s)	102, 705, 1186
Mph1103I	ATGCAT	2 cut(s)	6, 1489
MroI	TCCGGA	1 cut(s)	290
MseI	TTAA	3 cut(s)	1101, 1293, 1344
MslI	CAYNNNNRTG	1 cut(s)	1417
MspA1I	CMGCKG	2 cut(s)	673, 742
MspI	CCGG	5 cut(s)	248, 291, 701, 945, 1247
MspR9I	CCNGG	1 cut(s)	946
Mva1269I	GAATGC	1 cut(s)	480
MvnI	CGCG	3 cut(s)	543, 673, 815
NciI	CCSGG	1 cut(s)	946
NdeI	CATATG	1 cut(s)	1414
NlaIII	CATG	3 cut(s)	58, 667, 1608
NlaIV	GGNNCC	5 cut(s)	70, 699, 857, 1114, 1251
NmeAIII	GCCGAG	3 cut(s)	202, 212, 360
NsiI	ATGCAT	2 cut(s)	6, 1489
NspI	RCATGY	1 cut(s)	667
PaeR7I	CTCGAG	1 cut(s)	615
PceI	AGGCCT	1 cut(s)	1566
PciI	ACATGT	1 cut(s)	663
PcsI	WCGNNNNNNNCGW	3 cut(s)	102, 282, 622
PctI	GAATGC	1 cut(s)	480
PfeI	GAWTC	6 cut(s)	379, 446, 941, 950, 998, 1572
PflFI	GACNNNGTC	2 cut(s)	272, 710
PflMI	CCANNNNNTGG	1 cut(s)	1178
Ple19I	CGATCG	1 cut(s)	1408
PleI	GAGTC	1 cut(s)	607
PpsI	GAGTC	1 cut(s)	607
PscI	ACATGT	1 cut(s)	663
PsiI	TTATAA	1 cut(s)	375
PspN4I	GGNNCC	5 cut(s)	70, 699, 857, 1114, 1251
PspPI	GGNCC	3 cut(s)	386, 1113, 1249
PstI	CTGCAG	1 cut(s)	1033
PsuI	RGATCY	4 cut(s)	74, 675, 1199, 1312
PsyI	GACNNNGTC	2 cut(s)	272, 710
PvuI	CGATCG	1 cut(s)	1408
RsaI	GTAC	1 cut(s)	302
RsaNI	GTAC	1 cut(s)	301
RseI	CAYNNNNRTG	1 cut(s)	1417
SacII	CCGCGG	1 cut(s)	674
SalI	GTCGAC	2 cut(s)	251, 704
SaqAI	TTAA	3 cut(s)	1101, 1293, 1344
Sau96I	GGNCC	3 cut(s)	386, 1113, 1249
SchI	GAGTC	1 cut(s)	607
ScrFI	CCNGG	1 cut(s)	946
SfaNI	GCATC	6 cut(s)	13, 27, 102, 441, 1015, 1496
SfcI	CTRYAG	2 cut(s)	133, 1029
Sfr274I	CTCGAG	1 cut(s)	615
Sfr303I	CCGCGG	1 cut(s)	674
SgrBI	CCGCGG	1 cut(s)	674
SinI	GGWCC	2 cut(s)	1113, 1249
SlaI	CTCGAG	1 cut(s)	615
SmiMI	CAYNNNNRTG	1 cut(s)	1417
SmlI	CTYRAG	3 cut(s)	524, 615, 839
SmoI	CTYRAG	3 cut(s)	524, 615, 839
Sse9I	AATT	4 cut(s)	294, 313, 974, 1503
SseBI	AGGCCT	1 cut(s)	1566
SspMI	CTAG	1 cut(s)	957
StuI	AGGCCT	1 cut(s)	1566
StyD4I	CCNGG	1 cut(s)	944
TaaI	ACNGT	4 cut(s)	409, 879, 913, 933
TaiI	ACGT	1 cut(s)	258
TasI	AATT	4 cut(s)	294, 313, 974, 1503
TatI	WGTACW	1 cut(s)	300
TauI	GCSGC	7 cut(s)	191, 206, 334, 457, 501, 745, 815
TfiI	GAWTC	6 cut(s)	379, 446, 941, 950, 998, 1572
Tru1I	TTAA	3 cut(s)	1101, 1293, 1344
Tru9I	TTAA	3 cut(s)	1101, 1293, 1344
TscAI	CASTG	3 cut(s)	857, 918, 985
TspDTI	ATGAA	4 cut(s)	1029, 1062, 1164, 1431
TspGWI	ACGGA	3 cut(s)	177, 372, 800
TspRI	CASTG	3 cut(s)	857, 918, 985
Tth111I	GACNNNGTC	2 cut(s)	272, 710
Van91I	CCANNNNNTGG	1 cut(s)	1178
VpaK11BI	GGWCC	2 cut(s)	1113, 1249
XagI	CCTNNNNNAGG	1 cut(s)	235
XapI	RAATTY	3 cut(s)	294, 974, 1503
XbaI	TCTAGA	1 cut(s)	956
XceI	RCATGY	1 cut(s)	667
XhoI	CTCGAG	1 cut(s)	615
XmiI	GTMKAC	2 cut(s)	252, 705
XspI	CTAG	1 cut(s)	957
ZraI	GACGTC	1 cut(s)	256
Zsp2I	ATGCAT	2 cut(s)	6, 1489

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.