pycom15g32150

Transmembrane protein 50 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0015086
Physical Location & Seq
Forward (+)
31061046 .. 31062336
1291 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 408 bp
ATGGATTTGTCGGAGCTCTGGGGGATCTTCGGTCCTGGCGTTGCCGGAGCCGTGTTCGGGGCCGGCTGGTGGTTCTGGGTTGACGCTGTCGTTTGCAGTTCTGTCAAGGTCGCCTTCCTCCACTACCTCCCTGGCATATTTGCGTCGATAGCGGCGCTGATGTTCAATTGCGTCAAAAAGGAGGACATTGATTACTCCCCTTATGAAGAAGGCGAGTGGAGATTGAAGCTTTGGCTGTTCATTGCTTATGTTGTTGCATTTGTATCTCTAGCTGCATCAGTGGGTCTGTTAATACAAGATTCTTTAGTGAAGACTGGCCCTTCAGTGTGGACTGGAACTGCTGGTGTGTTGCAAGCAGTTTTTGTGTTGATCAGTGGGCTAATTTATTGGACTGCTCACCCGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UPF0220

Protein Analysis

136

Amino Acids

14.77

Weight (kDa)

4.79

Isoelectric Point (pI)

20.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPF0220 PF05255 13 - 133 5.5e-24 Uncharacterised protein family (UPF0220)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015086)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G36980 AT1G36980
fragaria_vesca FvH4_5g38950
malus_domestica MD15G1359200.v1.1
prunus_persica Prupe.1G506000_v2.0.a1 Prupe.1G506000_v2.0.a1
pyrus_communis pycom15g32150
rosa_chinensis RchiOBHm_Chr7g0240921
rosa_multiflora Rmu_sc0004821.1_g000025
rosa_roxburghii Rroxscaffold_3G00219420
rosa_rugosa Rorug07G0340100 Rorug07G0340200
rosa_samantha Rh7BG466300 Rh7CG512800 Rh7DG479600
rosa_wichuraiana Rw7G041840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 152
AclWI GGATC 1 cut(s) 32
AcuI CTGAAG 1 cut(s) 306
AfiI CCNNNNNNNGG 2 cut(s) 57, 69
AgsI TTSAA 2 cut(s) 166, 226
AjnI CCWGG 2 cut(s) 34, 130
AluBI AGCT 3 cut(s) 16, 229, 272
AluI AGCT 3 cut(s) 16, 229, 272
Alw21I GWGCWC 1 cut(s) 18
AlwI GGATC 1 cut(s) 32
AoxI GGCC 2 cut(s) 60, 316
ApeKI GCWGC 1 cut(s) 272
AspLEI GCGC 1 cut(s) 157
AspS9I GGNCC 3 cut(s) 32, 60, 317
AsuC2I CCSGG 1 cut(s) 401
AsuHPI GGTGA 1 cut(s) 389
AvaII GGWCC 1 cut(s) 32
BanII GRGCYC 1 cut(s) 18
BbsI GAAGAC 1 cut(s) 317
Bbv12I GWGCWC 1 cut(s) 18
BbvI GCAGC 1 cut(s) 259
BceAI ACGGC 1 cut(s) 35
BciT130I CCWGG 2 cut(s) 36, 132
BclI TGATCA 1 cut(s) 369
BcnI CCSGG 1 cut(s) 401
BfaI CTAG 1 cut(s) 269
BfoI RGCGCY 1 cut(s) 158
BisI GCNGC 2 cut(s) 153, 273
BlsI GCNGC 2 cut(s) 154, 274
Bme1390I CCNGG 3 cut(s) 36, 132, 401
Bme18I GGWCC 1 cut(s) 32
BmgT120I GGNCC 3 cut(s) 32, 60, 317
BmiI GGNNCC 2 cut(s) 49, 61
BmrFI CCNGG 3 cut(s) 36, 132, 401
BmsI GCATC 1 cut(s) 284
BpiI GAAGAC 1 cut(s) 317
BpuMI CCSGG 1 cut(s) 401
BsaJI CCNNGG 1 cut(s) 130
Bsc4I CCNNNNNNNGG 2 cut(s) 57, 69
Bse118I RCCGGY 1 cut(s) 62
Bse1I ACTGG 2 cut(s) 319, 337
Bse3DI GCAATG 1 cut(s) 240
BseBI CCWGG 2 cut(s) 36, 132
BseDI CCNNGG 1 cut(s) 130
BseLI CCNNNNNNNGG 2 cut(s) 57, 69
BseMI GCAATG 1 cut(s) 240
BseNI ACTGG 2 cut(s) 319, 337
BseXI GCAGC 1 cut(s) 259
BshFI GGCC 2 cut(s) 62, 318
BsiHKAI GWGCWC 1 cut(s) 18
BsiSI CCGG 3 cut(s) 45, 63, 401
BslI CCNNNNNNNGG 2 cut(s) 57, 69
BsnI GGCC 2 cut(s) 62, 318
Bsp1286I GDGCHC 1 cut(s) 18
Bsp143I GATC 2 cut(s) 24, 369
BspACI CCGC 1 cut(s) 152
BspANI GGCC 2 cut(s) 62, 318
BspLI GGNNCC 2 cut(s) 49, 61
BspPI GGATC 1 cut(s) 32
BsrDI GCAATG 1 cut(s) 240
BsrFI RCCGGY 1 cut(s) 62
BsrI ACTGG 2 cut(s) 319, 337
BssAI RCCGGY 1 cut(s) 62
BssECI CCNNGG 1 cut(s) 130
BssMI GATC 2 cut(s) 24, 369
Bst2UI CCWGG 2 cut(s) 36, 132
BstC8I GCNNGC 2 cut(s) 64, 354
BstH2I RGCGCY 1 cut(s) 158
BstHHI GCGC 1 cut(s) 157
BstKTI GATC 2 cut(s) 27, 372
BstMBI GATC 2 cut(s) 24, 369
BstMWI GCNNNNNNNGC 1 cut(s) 149
BstNI CCWGG 2 cut(s) 36, 132
BstSCI CCNGG 3 cut(s) 34, 130, 399
BstV1I GCAGC 1 cut(s) 259
BstV2I GAAGAC 1 cut(s) 317
BstX2I RGATCY 1 cut(s) 24
BstYI RGATCY 1 cut(s) 24
BsuRI GGCC 2 cut(s) 62, 318
BtsIMutI CAGTG 3 cut(s) 285, 330, 379
Cac8I GCNNGC 2 cut(s) 64, 354
CfoI GCGC 1 cut(s) 157
Cfr10I RCCGGY 1 cut(s) 62
Cfr13I GGNCC 3 cut(s) 32, 60, 317
CseI GACGC 3 cut(s) 92, 132, 160
CviJI RGCY 9 cut(s) 16, 50, 62, 66, 229, 235, 272, 318, 379
CviKI_1 RGCY 9 cut(s) 16, 50, 62, 66, 229, 235, 272, 318, 379
DpnI GATC 2 cut(s) 26, 371
DpnII GATC 2 cut(s) 24, 369
Ecl136II GAGCTC 1 cut(s) 16
Eco24I GRGCYC 1 cut(s) 18
Eco47I GGWCC 1 cut(s) 32
Eco53kI GAGCTC 1 cut(s) 16
Eco57I CTGAAG 1 cut(s) 306
EcoICRI GAGCTC 1 cut(s) 16
EcoRII CCWGG 2 cut(s) 34, 130
EcoT38I GRGCYC 1 cut(s) 18
FaiI YATR 3 cut(s) 137, 204, 249
FalI AAGNNNNNCTT 2 cut(s) 98, 130
FbaI TGATCA 1 cut(s) 369
Fnu4HI GCNGC 2 cut(s) 153, 273
FriOI GRGCYC 1 cut(s) 18
Fsp4HI GCNGC 2 cut(s) 153, 273
FspBI CTAG 1 cut(s) 269
GlaI GCGC 1 cut(s) 156
GluI GCNGC 2 cut(s) 153, 273
HaeII RGCGCY 1 cut(s) 158
HaeIII GGCC 2 cut(s) 62, 318
HapII CCGG 3 cut(s) 45, 63, 401
HgaI GACGC 3 cut(s) 92, 132, 160
HhaI GCGC 1 cut(s) 157
Hin6I GCGC 1 cut(s) 155
HinP1I GCGC 1 cut(s) 155
HincII GTYRAC 1 cut(s) 82
HindII GTYRAC 1 cut(s) 82
HindIII AAGCTT 1 cut(s) 227
HinfI GANTC 1 cut(s) 299
HpaII CCGG 3 cut(s) 45, 63, 401
HphI GGTGA 1 cut(s) 389
Hpy166II GTNNAC 2 cut(s) 82, 330
Hpy188I TCNGA 1 cut(s) 13
Hpy8I GTNNAC 2 cut(s) 82, 330
Hpy99I CGWCG 1 cut(s) 148
HpyAV CCTTC 3 cut(s) 124, 203, 330
HpyCH4V TGCA 4 cut(s) 96, 257, 275, 352
HpyF10VI GCNNNNNNNGC 1 cut(s) 149
HspAI GCGC 1 cut(s) 155
KroI GCCGGC 1 cut(s) 62
KroNI GCCGGC 1 cut(s) 64
Ksp22I TGATCA 1 cut(s) 369
Kzo9I GATC 2 cut(s) 24, 369
LmnI GCTCC 2 cut(s) 13, 47
Lsp1109I GCAGC 1 cut(s) 259
LweI GCATC 1 cut(s) 284
MaeI CTAG 1 cut(s) 269
MalI GATC 2 cut(s) 26, 371
MboI GATC 2 cut(s) 24, 369
MboII GAAGA 3 cut(s) 19, 218, 322
MfeI CAATTG 1 cut(s) 166
MflI RGATCY 1 cut(s) 24
MhlI GDGCHC 1 cut(s) 18
MluCI AATT 2 cut(s) 166, 381
MnlI CCTC 3 cut(s) 128, 137, 175
MroNI GCCGGC 1 cut(s) 62
MseI TTAA 1 cut(s) 290
MspI CCGG 3 cut(s) 45, 63, 401
MspR9I CCNGG 3 cut(s) 36, 132, 401
MunI CAATTG 1 cut(s) 166
MvaI CCWGG 2 cut(s) 36, 132
MwoI GCNNNNNNNGC 1 cut(s) 149
NaeI GCCGGC 1 cut(s) 64
NciI CCSGG 1 cut(s) 401
NdeII GATC 2 cut(s) 24, 369
NgoMIV GCCGGC 1 cut(s) 62
NlaIV GGNNCC 2 cut(s) 49, 61
PcsI WCGNNNNNNNCGW 1 cut(s) 36
PdiI GCCGGC 1 cut(s) 64
PfeI GAWTC 1 cut(s) 299
PflFI GACNNNGTC 1 cut(s) 86
PkrI GCNGC 2 cut(s) 154, 274
Psp124BI GAGCTC 1 cut(s) 18
Psp6I CCWGG 2 cut(s) 34, 130
PspGI CCWGG 2 cut(s) 34, 130
PspN4I GGNNCC 2 cut(s) 49, 61
PspPI GGNCC 3 cut(s) 32, 60, 317
PsuI RGATCY 1 cut(s) 24
PsyI GACNNNGTC 1 cut(s) 86
SacI GAGCTC 1 cut(s) 18
SaqAI TTAA 1 cut(s) 290
SatI GCNGC 2 cut(s) 153, 273
Sau3AI GATC 2 cut(s) 24, 369
Sau96I GGNCC 3 cut(s) 32, 60, 317
ScrFI CCNGG 3 cut(s) 36, 132, 401
SduI GDGCHC 1 cut(s) 18
SetI ASST 5 cut(s) 18, 111, 129, 231, 274
SfaNI GCATC 1 cut(s) 284
SinI GGWCC 1 cut(s) 32
Sse9I AATT 2 cut(s) 166, 381
SsiI CCGC 1 cut(s) 152
SspMI CTAG 1 cut(s) 269
SstI GAGCTC 1 cut(s) 18
StyD4I CCNGG 3 cut(s) 34, 130, 399
TaqI TCGA 1 cut(s) 146
TaqII GACCGA 1 cut(s) 20
TasI AATT 2 cut(s) 166, 381
TauI GCSGC 1 cut(s) 155
TfiI GAWTC 1 cut(s) 299
Tru1I TTAA 1 cut(s) 290
Tru9I TTAA 1 cut(s) 290
TscAI CASTG 3 cut(s) 285, 330, 379
TseI GCWGC 1 cut(s) 272
TspDTI ATGAA 2 cut(s) 219, 229
TspRI CASTG 3 cut(s) 285, 330, 379
Tth111I GACNNNGTC 1 cut(s) 86
VpaK11BI GGWCC 1 cut(s) 32
XcmI CCANNNNNNNNNTGG 1 cut(s) 128
XspI CTAG 1 cut(s) 269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.