pycom15g32750

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0014594
Physical Location & Seq
Reverse (-)
31940906 .. 31946387
5482 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2460 bp
ATGAAAACTTACTCAAAAACGAACATTTTAACGGCTGCCCTTATAAACTCTCCCTCTCTTTCATTCCTTCTGCGTTCATTTCTGCATCTAACCAAACCACTCACAATGAAACTCCATTCACACGCTCCTCCTCCCTCAGCCCTTATCTTCTTCTTCACCACCGCCCTCCTCCTCCTCCACCCTACCGAGTCAACTCAGCCTCCATATTCCTGTGACTCGTCCCAACCCTCCACCAGCTCCTACCCTTTTTGCAAAACCACTCTCCCCATCAACCAACGAGTCCAGGACCTCATCTCCCGCCTCACATTGGACGAGAAAATCTCCCAACTCGTTAACTCGGCCCCTCCGATTCCCCGACTCGGTATCCCGAGTTACGAGTGGTGGTCCGAGGCTTTACACGGCGTCGCCGACGTCGGCAAAGGCATTAAACTTTACCCCACCATTCACAACGCCACGAGCTTCCCTCAAGTCATCCTCACCGCCGCTTCTTTCAACGACCATCTCTGGTACCGCATTGGCCAGGCGATTGGGACTGAAGCTCGAGCAGTGTACAATGCCGGACAAGCAACAGGGATGACATTTTGGGCACCTAATATCAACATCTTCAGGGACCCGAGATGGGGGAGAGGGCAAGAGACCCCTGGCGAAGATCCAACGGTCACAGGGAGATACGCCGTGTCGTATGTGAGAGGGGTTCAAGGTGATTCCTTCTATGGCGGGAAGCATAAGCTTGGTGCCGGTGGACGTCTTCAAGCTTCAGCTTGCTGCAAGCATTTCACAGCTTACGATTTGGACAATTGGAACAATATCACTCGATTTGGCTTCGACGCTCGTGTGACACAGCAAGACCTAGCAGACACATACCAGCCTCCGTTCCAAAAATGCGTGCAGGAAGGCCAAGCAAGTGGCTTAATGTGTGCCTATAACCGCATCAATGGGGTTCCAAGCTGTGCAGATCACAATCTCCTAACCAAGGTTGCAAGAGGACAATGGGACTTTCATGGGTACATCACGTCGGACTGCGATGCTGTCTCCATCATCTACGATGTACAAGGATACGCTAAAAAGCCTGAGGATGCAGTCGTTGATGTGCTCAAAGCAGGCATGGATGTGAACTGTGGATCCTACTTGAAGGATCACACAAAATCAGCAGTGGAGCAGAAAAAGCTTGAAGTGTCAAAAATAGACAGAGCACTCCACAACCTCTTCTCAGTGAGGATGAGGCTAGGCCTGTTTGATGGCAGTCCACTCAACCAACCCTATGGCAGCATTGGTCCAGACCAAGTGTGCTCCCAACTGCACCAGGCTCTAGCCCTAGAAGCTGCTCAAGATGGAATAGTGCTTTTGAAAAACTCTGGCAGGCTTCTCCCACTCCCAAAAGCAAAAGCCATTTCACTTGCTGTCATTGGTCCCCATGCCAATGCTTTAGAAGCACTTCTGGGAAACTACCATGGTCCTCCATGCAAATCCATCACCCCATTAGAAGCACTTCAAGGCTACGCTAAGAACATAAATTATCACCTGGGTTGTGATTCAGTTGGGTGTCCCCAAGCTGCTTTTGATGAAGCTGCTGGAACAGCAAAAGAAGCAGATTATGTGGTTTTGATTGTGGGATTGGACCAAAGTCAAGAGAGAGAGGAACTTGATAGATTGCAATTGGGATTGCCTGGGAAGCAGCAGGAGCTAATTAGGAGTGTGGCTAAAGTTGCCAAGAAACCTGTTATTCTCGTGATTCTTAGTGGGGGTCCAGTTGACATTTCTTTTGCTAAACATAACAAAAATATTGGAAGCATCTTGTGGGCTGGATATCCAGGTGAAGCTGGTGGAATTGCCCTTGCTGAAATCATCTTTGGTGAACACAACCCAGGTGGAAGATTGCCAATGGCTTGGTACACACAAGATTATGTCAAAATGCCAATGACAGACATGAGAATGAGGTGTGAACCGAAAACAGGCTATCCTGGTCGAACTTACAGATTCTACACAGGCAAAAAAGTTTTTGACTTTGGTTATGGCCTCAGCTACTCAAACTATGTTTATAAGTTTTCCTCTGCAGCATCCCAAAACAAGCTCTACTTAAATGAATCATCAATTAGTCCCACGGCTAAGTCATCGGACTCTGGGCGGTATCAGTTAGTCCAAGACCTAGGTGGAGAATTCTGCGAGAAAAAGAAGTTATCGGTCAAAGTGAGGGTTCACAATCATGGGGAGATGGCAGGCAAGCATCCGGTGCTGCTTTTTGTGGGTGAGAAAAAGCCCAAAAATGGAAGGCCAATGAAGCGGTTGGTTGGATTCAAAAGTGTGATACTGAGTGCTGGGGAAAGAGCTGAAATTGAATTTGATTTGAACCCTTGTGAGCACTTGAGCCATGCAAATGAGGAAGGTTTGATGGCGGTTGAAGAAGGTTCTTATTTCTTGGGCGTAGGAGATGCAAAACACCCTCTTGACATCGTAGTGTGA

Protein Analysis

820

Amino Acids

89.78

Weight (kDa)

8.47

Isoelectric Point (pI)

37.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_3 PF00933 148 - 403 6.1e-31 Glycosyl hydrolase family 3 N terminal domain
Glyco_hydro_3_C PF01915 446 - 675 1.3e-51 Glycosyl hydrolase family 3 C-terminal domain
Fn3-like PF14310 744 - 810 3.6e-11 Fibronectin type III-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014594)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G78060
fragaria_vesca FvH4_5g37840
malus_domestica MD08G1179900.v1.1 MD15G1365200.v1.1
prunus_persica Prupe.1G513200_v2.0.a1
pyrus_communis pycom08g15400 pycom15g32750
rosa_chinensis RchiOBHm_Chr7g0239821
rosa_laevigata RLG00000000728
rosa_multiflora Rmu_sc0007193.1_g000008
rosa_roxburghii Rroxscaffold_3G00220790
rosa_rugosa Rorug07G0329500
rosa_samantha Rh7AG483700 Rh7BG456700 Rh7CG501200 Rh7DG469400
rosa_wichuraiana Rw7G040570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 44, 2040
AatII GACGTC 2 cut(s) 414, 748
Acc65I GGTACC 1 cut(s) 507
AccB1I GGYRCC 3 cut(s) 507, 586, 734
AclWI GGATC 4 cut(s) 644, 1116, 1129, 1143
AcoI YGGCCR 1 cut(s) 517
AcsI RAATTY 2 cut(s) 2156, 2336
AcuI CTGAAG 3 cut(s) 555, 589, 741
AcyI GRCGYC 3 cut(s) 402, 411, 745
AfaI GTAC 5 cut(s) 509, 551, 1007, 1050, 1892
AfiI CCNNNNNNNGG 7 cut(s) 307, 359, 619, 620, 973, 1952, 2264
AjiI CACGTC 1 cut(s) 1014
AjnI CCWGG 9 cut(s) 282, 519, 640, 1302, 1521, 1666, 1810, 1864, 1960
AjuI GAANNNNNNNTTGG 2 cut(s) 1833, 1865
AloI GAACNNNNNNTCC 2 cut(s) 1106, 1138
Alw21I GWGCWC 4 cut(s) 1095, 1195, 1292, 2361
Alw26I GTCTC 2 cut(s) 629, 1036
AlwI GGATC 4 cut(s) 644, 1116, 1129, 1143
Ama87I CYCGRG 3 cut(s) 367, 540, 613
AoxI GGCC 6 cut(s) 339, 517, 895, 1228, 2014, 2270
ApeKI GCWGC 9 cut(s) 35, 765, 1266, 1322, 1553, 1568, 1675, 2054, 2233
ApoI RAATTY 2 cut(s) 2156, 2336
ArsI GACNNNNNNTTYG 2 cut(s) 1745, 1777
Asp700I GAANNNNTTC 2 cut(s) 1434, 1488
Asp718I GGTACC 1 cut(s) 507
AspA2I CCTAGG 1 cut(s) 2146
AspS9I GGNCC 9 cut(s) 286, 340, 384, 610, 1274, 1409, 1454, 1618, 1745
AsuHPI GGTGA 8 cut(s) 148, 469, 713, 1465, 1511, 1826, 1865, 2258
AvaI CYCGRG 3 cut(s) 367, 540, 613
AvaII GGWCC 8 cut(s) 286, 384, 610, 1274, 1409, 1454, 1618, 1745
AvrII CCTAGG 1 cut(s) 2146
AxyI CCTNAGG 1 cut(s) 1071
BaeGI GKGCMC 1 cut(s) 589
BalI TGGCCA 1 cut(s) 519
BamHI GGATCC 1 cut(s) 1121
BanI GGYRCC 3 cut(s) 507, 586, 734
BauI CACGAG 3 cut(s) 454, 831, 1727
BbsI GAAGAC 1 cut(s) 740
Bbv12I GWGCWC 4 cut(s) 1095, 1195, 1292, 2361
BbvCI CCTCAGC 2 cut(s) 136, 2018
BbvI GCAGC 9 cut(s) 22, 752, 1278, 1309, 1540, 1555, 1687, 2066, 2220
BccI CCATC 9 cut(s) 275, 507, 612, 1043, 1232, 1325, 1478, 2206, 2383
BceAI ACGGC 4 cut(s) 48, 415, 659, 2118
BciT130I CCWGG 9 cut(s) 284, 521, 642, 1304, 1523, 1668, 1812, 1866, 1962
BciVI GTATCC 2 cut(s) 374, 1049
BcoDI GTCTC 2 cut(s) 629, 1036
BfaI CTAG 5 cut(s) 851, 1226, 1310, 1316, 2147
BfmI CTRYAG 1 cut(s) 2052
BfuI GTATCC 2 cut(s) 374, 1049
BlnI CCTAGG 1 cut(s) 2146
Bme1390I CCNGG 9 cut(s) 284, 521, 642, 1304, 1523, 1668, 1812, 1866, 1962
Bme18I GGWCC 8 cut(s) 286, 384, 610, 1274, 1409, 1454, 1618, 1745
BmeT110I CYCGRG 3 cut(s) 367, 540, 613
BmgBI CACGTC 1 cut(s) 1014
BmgT120I GGNCC 9 cut(s) 286, 340, 384, 610, 1274, 1409, 1454, 1618, 1745
BmrFI CCNGG 9 cut(s) 284, 521, 642, 1304, 1523, 1668, 1812, 1866, 1962
BmsI GCATC 8 cut(s) 94, 939, 1015, 1066, 1800, 2066, 2233, 2419
BoxI GACNNNNGTC 1 cut(s) 1623
BpiI GAAGAC 1 cut(s) 740
BplI GAGNNNNNCTC 4 cut(s) 305, 337, 448, 480
Bpu10I CCTNAGC 2 cut(s) 136, 2018
BpuEI CTTGAG 3 cut(s) 450, 1311, 2383
BsaBI GATNNNNATC 1 cut(s) 960
BsaHI GRCGYC 3 cut(s) 402, 411, 745
BsaI GGTCTC 1 cut(s) 629
BsaJI CCNNGG 9 cut(s) 387, 640, 972, 1450, 1522, 1667, 1864, 2100, 2146
BsaWI WCCGGW 1 cut(s) 2227
BsaXI ACNNNNNCTCC 4 cut(s) 184, 214, 278, 308
Bsc4I CCNNNNNNNGG 7 cut(s) 307, 359, 619, 620, 973, 1952, 2264
Bse118I RCCGGY 1 cut(s) 737
Bse1I ACTGG 1 cut(s) 1748
Bse21I CCTNAGG 1 cut(s) 1071
Bse8I GATNNNNATC 1 cut(s) 960
BseBI CCWGG 9 cut(s) 284, 521, 642, 1304, 1523, 1668, 1812, 1866, 1962
BseDI CCNNGG 9 cut(s) 387, 640, 972, 1450, 1522, 1667, 1864, 2100, 2146
BseGI GGATG 7 cut(s) 471, 579, 1081, 1114, 1224, 2057, 2224
BseJI GATNNNNATC 1 cut(s) 960
BseLI CCNNNNNNNGG 7 cut(s) 307, 359, 619, 620, 973, 1952, 2264
BseMII CTCAG 6 cut(s) 150, 209, 1062, 1224, 2032, 2300
BseNI ACTGG 1 cut(s) 1748
BseRI GAGGAG 5 cut(s) 117, 120, 158, 161, 164
BseSI GKGCMC 1 cut(s) 589
BseXI GCAGC 9 cut(s) 22, 752, 1278, 1309, 1540, 1555, 1687, 2066, 2220
BseYI CCCAGC 1 cut(s) 2315
BsgI GTGCAG 3 cut(s) 908, 972, 1283
BshFI GGCC 6 cut(s) 341, 519, 897, 1230, 2016, 2272
BshNI GGYRCC 3 cut(s) 507, 586, 734
BsiHKAI GWGCWC 4 cut(s) 1095, 1195, 1292, 2361
BsiHKCI CYCGRG 3 cut(s) 367, 540, 613
BsiSI CCGG 3 cut(s) 558, 738, 2228
BslFI GGGAC 7 cut(s) 205, 544, 623, 1007, 1395, 1530, 2082
BslI CCNNNNNNNGG 7 cut(s) 307, 359, 619, 620, 973, 1952, 2264
BsmAI GTCTC 2 cut(s) 629, 1036
BsmFI GGGAC 7 cut(s) 205, 544, 623, 1007, 1395, 1530, 2082
BsnI GGCC 6 cut(s) 341, 519, 897, 1230, 2016, 2272
Bso31I GGTCTC 1 cut(s) 629
BsoBI CYCGRG 3 cut(s) 367, 540, 613
Bsp1286I GDGCHC 5 cut(s) 589, 1095, 1195, 1292, 2361
Bsp1407I TGTACA 2 cut(s) 549, 1048
Bsp143I GATC 4 cut(s) 649, 955, 1121, 1135
Bsp19I CCATGG 1 cut(s) 1450
BspANI GGCC 6 cut(s) 341, 519, 897, 1230, 2016, 2272
BspCNI CTCAG 6 cut(s) 149, 208, 1063, 1223, 2031, 2301
BspMAI CTGCAG 1 cut(s) 2056
BspPI GGATC 4 cut(s) 644, 1116, 1129, 1143
BspT107I GGYRCC 3 cut(s) 507, 586, 734
BspTNI GGTCTC 1 cut(s) 629
BsrFI RCCGGY 1 cut(s) 737
BsrGI TGTACA 2 cut(s) 549, 1048
BsrI ACTGG 1 cut(s) 1748
BssAI RCCGGY 1 cut(s) 737
BssECI CCNNGG 9 cut(s) 387, 640, 972, 1450, 1522, 1667, 1864, 2100, 2146
BssMI GATC 4 cut(s) 649, 955, 1121, 1135
BssNI GRCGYC 3 cut(s) 402, 411, 745
BssSI CACGAG 3 cut(s) 454, 831, 1727
BssT1I CCWWGG 3 cut(s) 972, 1450, 2146
Bst2BI CACGAG 3 cut(s) 454, 831, 1727
Bst2UI CCWGG 9 cut(s) 284, 521, 642, 1304, 1523, 1668, 1812, 1866, 1962
Bst4CI ACNGT 2 cut(s) 658, 1118
Bst6I CTCTTC 1 cut(s) 1211
BstACI GRCGYC 3 cut(s) 402, 411, 745
BstAPI GCANNNNNTGC 1 cut(s) 2230
BstAUI TGTACA 2 cut(s) 549, 1048
BstC8I GCNNGC 7 cut(s) 763, 770, 887, 1102, 1361, 2218, 2222
BstDEI CTNAG 9 cut(s) 136, 195, 1071, 1210, 1503, 1736, 2018, 2106, 2309
BstDSI CCRYGG 2 cut(s) 1450, 2100
BstENI CCTNNNNNAGG 1 cut(s) 971
BstF5I GGATG 7 cut(s) 471, 579, 1081, 1114, 1224, 2057, 2224
BstKTI GATC 4 cut(s) 652, 958, 1124, 1138
BstMAI GTCTC 2 cut(s) 629, 1036
BstMBI GATC 4 cut(s) 649, 955, 1121, 1135
BstNI CCWGG 9 cut(s) 284, 521, 642, 1304, 1523, 1668, 1812, 1866, 1962
BstPAI GACNNNNGTC 1 cut(s) 1623
BstSCI CCNGG 9 cut(s) 282, 519, 640, 1302, 1521, 1666, 1810, 1864, 1960
BstSFI CTRYAG 1 cut(s) 2052
BstSLI GKGCMC 1 cut(s) 589
BstV1I GCAGC 9 cut(s) 22, 752, 1278, 1309, 1540, 1555, 1687, 2066, 2220
BstV2I GAAGAC 1 cut(s) 740
BstX2I RGATCY 2 cut(s) 649, 1121
BstXI CCANNNNNNTGG 4 cut(s) 527, 905, 1262, 1887
BstYI RGATCY 2 cut(s) 649, 1121
Bsu36I CCTNAGG 1 cut(s) 1071
BsuI GTATCC 2 cut(s) 374, 1049
BsuRI GGCC 6 cut(s) 341, 519, 897, 1230, 2016, 2272
BtgI CCRYGG 2 cut(s) 1450, 2100
BtgZI GCGATG 1 cut(s) 1038
BtrI CACGTC 1 cut(s) 1014
BtsCI GGATG 7 cut(s) 471, 579, 1081, 1114, 1224, 2057, 2224
BtsI GCAGTG 2 cut(s) 552, 1158
BtsIMutI CAGTG 3 cut(s) 552, 1158, 1218
Cac8I GCNNGC 7 cut(s) 763, 770, 887, 1102, 1361, 2218, 2222
Cfr10I RCCGGY 1 cut(s) 737
Cfr13I GGNCC 9 cut(s) 286, 340, 384, 610, 1274, 1409, 1454, 1618, 1745
CseI GACGC 2 cut(s) 391, 836
Csp6I GTAC 5 cut(s) 508, 550, 1006, 1049, 1891
CspCI CAANNNNNGTGG 2 cut(s) 1849, 1884
CviAII CATG 8 cut(s) 1001, 1105, 1415, 1451, 1461, 1927, 2204, 2369
CviQI GTAC 5 cut(s) 508, 550, 1006, 1049, 1891
DdeI CTNAG 9 cut(s) 136, 195, 1071, 1210, 1503, 1736, 2018, 2106, 2309
DpnI GATC 4 cut(s) 651, 957, 1123, 1137
DpnII GATC 4 cut(s) 649, 955, 1121, 1135
EaeI YGGCCR 1 cut(s) 517
Eam1104I CTCTTC 1 cut(s) 1211
EarI CTCTTC 1 cut(s) 1211
Eco130I CCWWGG 3 cut(s) 972, 1450, 2146
Eco147I AGGCCT 1 cut(s) 1230
Eco31I GGTCTC 1 cut(s) 629
Eco32I GATATC 1 cut(s) 1808
Eco47I GGWCC 8 cut(s) 286, 384, 610, 1274, 1409, 1454, 1618, 1745
Eco57I CTGAAG 3 cut(s) 555, 589, 741
Eco81I CCTNAGG 1 cut(s) 1071
Eco88I CYCGRG 3 cut(s) 367, 540, 613
EcoNI CCTNNNNNAGG 1 cut(s) 971
EcoO109I RGGNCCY 2 cut(s) 286, 610
EcoRI GAATTC 1 cut(s) 2156
EcoRII CCWGG 9 cut(s) 282, 519, 640, 1302, 1521, 1666, 1810, 1864, 1960
EcoRV GATATC 1 cut(s) 1808
EcoT14I CCWWGG 3 cut(s) 972, 1450, 2146
ErhI CCWWGG 3 cut(s) 972, 1450, 2146
FaeI CATG 8 cut(s) 1004, 1108, 1418, 1454, 1464, 1930, 2207, 2372
FalI AAGNNNNNCTT 2 cut(s) 2060, 2092
FaqI GGGAC 7 cut(s) 205, 544, 623, 1007, 1395, 1530, 2082
FatI CATG 8 cut(s) 1000, 1104, 1414, 1450, 1460, 1926, 2203, 2368
FauI CCCGC 2 cut(s) 305, 710
FokI GGATG 7 cut(s) 458, 586, 1088, 1121, 1231, 2044, 2211
FspBI CTAG 5 cut(s) 851, 1226, 1310, 1316, 2147
GsaI CCCAGC 1 cut(s) 2319
HaeIII GGCC 6 cut(s) 341, 519, 897, 1230, 2016, 2272
HapII CCGG 3 cut(s) 558, 738, 2228
HgaI GACGC 2 cut(s) 391, 836
Hin1I GRCGYC 3 cut(s) 402, 411, 745
Hin1II CATG 8 cut(s) 1004, 1108, 1418, 1454, 1464, 1930, 2207, 2372
HincII GTYRAC 3 cut(s) 192, 334, 1753
HindII GTYRAC 3 cut(s) 192, 334, 1753
HindIII AAGCTT 3 cut(s) 728, 753, 1166
HpaI GTTAAC 1 cut(s) 334
HpaII CCGG 3 cut(s) 558, 738, 2228
HphI GGTGA 8 cut(s) 148, 469, 713, 1465, 1511, 1826, 1865, 2258
Hpy188I TCNGA 4 cut(s) 348, 388, 1018, 2116
Hpy188III TCNNGA 6 cut(s) 367, 1277, 1328, 1628, 1729, 2444
Hpy99I CGWCG 5 cut(s) 407, 413, 416, 830, 1018
HpyAV CCTTC 7 cut(s) 77, 718, 887, 1126, 2262, 2375, 2396
HpyCH4III ACNGT 2 cut(s) 658, 1118
HpyCH4IV ACGT 3 cut(s) 411, 745, 1013
HpyF3I CTNAG 9 cut(s) 136, 195, 1071, 1210, 1503, 1736, 2018, 2106, 2309
HpySE526I ACGT 3 cut(s) 411, 745, 1013
Hsp92I GRCGYC 3 cut(s) 402, 411, 745
Hsp92II CATG 8 cut(s) 1004, 1108, 1418, 1454, 1464, 1930, 2207, 2372
KflI GGGWCCC 1 cut(s) 610
KpnI GGTACC 1 cut(s) 511
KspAI GTTAAC 1 cut(s) 334
Kzo9I GATC 4 cut(s) 649, 955, 1121, 1135
LmnI GCTCC 5 cut(s) 130, 242, 1156, 1295, 1681
Lsp1109I GCAGC 9 cut(s) 22, 752, 1278, 1309, 1540, 1555, 1687, 2066, 2220
LweI GCATC 8 cut(s) 94, 939, 1015, 1066, 1800, 2066, 2233, 2419
MaeI CTAG 5 cut(s) 851, 1226, 1310, 1316, 2147
MaeII ACGT 3 cut(s) 411, 745, 1013
MaeIII GTNAC 4 cut(s) 212, 371, 658, 835
MalI GATC 4 cut(s) 651, 957, 1123, 1137
MboI GATC 4 cut(s) 649, 955, 1121, 1135
MboII GAAGA 9 cut(s) 139, 142, 145, 595, 659, 740, 1198, 1884, 2411
MfeI CAATTG 2 cut(s) 796, 1655
MflI RGATCY 2 cut(s) 649, 1121
MhlI GDGCHC 5 cut(s) 589, 1095, 1195, 1292, 2361
MlsI TGGCCA 1 cut(s) 519
MluCI AATT 9 cut(s) 796, 1513, 1655, 1686, 1827, 2091, 2156, 2331, 2336
MluNI TGGCCA 1 cut(s) 519
MlyI GAGTC 5 cut(s) 197, 209, 288, 351, 2111
MmeI TCCRAC 3 cut(s) 677, 996, 2269
Mox20I TGGCCA 1 cut(s) 519
MroXI GAANNNNTTC 2 cut(s) 1434, 1488
MscI TGGCCA 1 cut(s) 519
MseI TTAA 5 cut(s) 29, 333, 426, 911, 2078
MslI CAYNNNNRTG 6 cut(s) 1109, 1419, 1931, 2202, 2373, 2453
Msp20I TGGCCA 1 cut(s) 519
MspI CCGG 3 cut(s) 558, 738, 2228
MspR9I CCNGG 9 cut(s) 284, 521, 642, 1304, 1523, 1668, 1812, 1866, 1962
MunI CAATTG 2 cut(s) 796, 1655
MvaI CCWGG 9 cut(s) 284, 521, 642, 1304, 1523, 1668, 1812, 1866, 1962
NcoI CCATGG 1 cut(s) 1450
NdeII GATC 4 cut(s) 649, 955, 1121, 1135
NlaIII CATG 8 cut(s) 1004, 1108, 1418, 1454, 1464, 1930, 2207, 2372
NmeAIII GCCGAG 1 cut(s) 317
NmuCI GTSAC 3 cut(s) 212, 658, 835
PaeR7I CTCGAG 1 cut(s) 540
PceI AGGCCT 1 cut(s) 1230
PcsI WCGNNNNNNNCGW 2 cut(s) 344, 405
PdmI GAANNNNTTC 2 cut(s) 1434, 1488
PfeI GAWTC 7 cut(s) 349, 704, 1532, 1732, 1977, 2084, 2292
PfoI TCCNGGA 1 cut(s) 282
PleI GAGTC 5 cut(s) 196, 209, 287, 351, 2111
PpsI GAGTC 5 cut(s) 196, 209, 287, 351, 2111
PpuMI RGGWCCY 2 cut(s) 286, 610
PshAI GACNNNNGTC 1 cut(s) 1623
PsiI TTATAA 2 cut(s) 44, 2040
Psp5II RGGWCCY 2 cut(s) 286, 610
Psp6I CCWGG 9 cut(s) 282, 519, 640, 1302, 1521, 1666, 1810, 1864, 1960
PspFI CCCAGC 1 cut(s) 2315
PspGI CCWGG 9 cut(s) 282, 519, 640, 1302, 1521, 1666, 1810, 1864, 1960
PspPI GGNCC 9 cut(s) 286, 340, 384, 610, 1274, 1409, 1454, 1618, 1745
PspPPI RGGWCCY 2 cut(s) 286, 610
PspXI VCTCGAGB 1 cut(s) 540
PstI CTGCAG 1 cut(s) 2056
PsuI RGATCY 2 cut(s) 649, 1121
RsaI GTAC 5 cut(s) 509, 551, 1007, 1050, 1892
RsaNI GTAC 5 cut(s) 508, 550, 1006, 1049, 1891
RseI CAYNNNNRTG 6 cut(s) 1109, 1419, 1931, 2202, 2373, 2453
SaqAI TTAA 5 cut(s) 29, 333, 426, 911, 2078
Sau3AI GATC 4 cut(s) 649, 955, 1121, 1135
Sau96I GGNCC 9 cut(s) 286, 340, 384, 610, 1274, 1409, 1454, 1618, 1745
SchI GAGTC 5 cut(s) 197, 209, 288, 351, 2111
ScrFI CCNGG 9 cut(s) 284, 521, 642, 1304, 1523, 1668, 1812, 1866, 1962
SduI GDGCHC 5 cut(s) 589, 1095, 1195, 1292, 2361
SfaNI GCATC 8 cut(s) 94, 939, 1015, 1066, 1800, 2066, 2233, 2419
SfcI CTRYAG 1 cut(s) 2052
Sfr274I CTCGAG 1 cut(s) 540
SinI GGWCC 8 cut(s) 286, 384, 610, 1274, 1409, 1454, 1618, 1745
SlaI CTCGAG 1 cut(s) 540
SmiMI CAYNNNNRTG 6 cut(s) 1109, 1419, 1931, 2202, 2373, 2453
SmlI CTYRAG 4 cut(s) 465, 540, 1326, 2362
SmoI CTYRAG 4 cut(s) 465, 540, 1326, 2362
Sse9I AATT 9 cut(s) 796, 1513, 1655, 1686, 1827, 2091, 2156, 2331, 2336
SseBI AGGCCT 1 cut(s) 1230
SspI AATATT 1 cut(s) 1783
SspMI CTAG 5 cut(s) 851, 1226, 1310, 1316, 2147
StuI AGGCCT 1 cut(s) 1230
StyD4I CCNGG 9 cut(s) 282, 519, 640, 1302, 1521, 1666, 1810, 1864, 1960
StyI CCWWGG 3 cut(s) 972, 1450, 2146
TaaI ACNGT 2 cut(s) 658, 1118
TaiI ACGT 3 cut(s) 414, 748, 1016
TaqI TCGA 4 cut(s) 541, 814, 825, 1966
TaqII GACCGA 1 cut(s) 2170
TasI AATT 9 cut(s) 796, 1513, 1655, 1686, 1827, 2091, 2156, 2331, 2336
TatI WGTACW 2 cut(s) 549, 1048
TauI GCSGC 1 cut(s) 485
TfiI GAWTC 7 cut(s) 349, 704, 1532, 1732, 1977, 2084, 2292
Tru1I TTAA 5 cut(s) 29, 333, 426, 911, 2078
Tru9I TTAA 5 cut(s) 29, 333, 426, 911, 2078
TscAI CASTG 3 cut(s) 552, 1158, 1218
TseFI GTSAC 3 cut(s) 212, 658, 835
TseI GCWGC 9 cut(s) 35, 765, 1266, 1322, 1553, 1568, 1675, 2054, 2233
Tsp45I GTSAC 3 cut(s) 212, 658, 835
TspDTI ATGAA 8 cut(s) 17, 51, 66, 122, 989, 1578, 2097, 2291
TspGWI ACGGA 1 cut(s) 861
TspRI CASTG 3 cut(s) 552, 1158, 1218
VpaK11BI GGWCC 8 cut(s) 286, 384, 610, 1274, 1409, 1454, 1618, 1745
XagI CCTNNNNNAGG 1 cut(s) 971
XapI RAATTY 2 cut(s) 2156, 2336
XcmI CCANNNNNNNNNTGG 1 cut(s) 2147
XhoI CTCGAG 1 cut(s) 540
XmaJI CCTAGG 1 cut(s) 2146
XmnI GAANNNNTTC 2 cut(s) 1434, 1488
XspI CTAG 5 cut(s) 851, 1226, 1310, 1316, 2147
ZraI GACGTC 2 cut(s) 412, 746
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.