pycom15g36670

Chaperone protein dnaJ 20, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0016939
Physical Location & Seq
Forward (+)
36634239 .. 36634880
642 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g36670.1

Sequence Viewer

Length: 642 bp
ATGTTCCTTGTATATATTAGCCTCAGTCCGCATAACTCTTTCCCATGCATTTTGCAGAACATTCACATAGCCATGAACACCTCTCTGCTGACTTCTTCAAGCTGCATAAACCCTAGTACCAATGCTGCAGCTGCTAAACCTAAACCTTCTTTTCTAAATTGTCATGCACGCAAAGCCACTAAAATTACATGCAGATCCACGAATAGTTACGAGAGCGGTAGTGCTAGTCATACCAATTTCTACAACCTACTTAGTTTGAATCCGAATACTAACACGAGCAAAGACGAGATCAAGCGAGCTTATAGAAGCATGGCGCTTCGCTATCATCCAGATGTATGTTATGATCAACAGAATTCTAATATTTTCACCGAGCGATTTGTGCAACTGAACGAGGCTTACAAGACGCTATTAGATCCTCTGTTGCGCGATGAATATGATTACGAGTTGGGTTTGAAAGATTATTGTAACAATAGTTGTAATAGGAGGACCACCTTCACCTACAGTATGGATAATTCTGTCAGAAATAATCAAGGTGCACAAAACAGATGGAGTCAACAAATTCTTGAGTTGAAGAGAAGGTCACAAAAGGAAGGATCATGGGCCAGCAGAATGAGGAGAGCTCGCGATATATCAAACCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

214

Amino Acids

24.56

Weight (kDa)

9.38

Isoelectric Point (pI)

54.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 79 - 146 1.5e-16 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016939)

Species Orthologous Gene IDs
malus_domestica MD08G1221200.v1.1
prunus_persica Prupe.1G558200_v2.0.a1
pyrus_communis pycom15g36670
rosa_chinensis RchiOBHm_Chr7g0226211
rosa_laevigata RLG00000001758
rosa_multiflora Rmu_sc0001350.1_g000004 Rmu_sc0013267.1_g000006
rosa_roxburghii Rroxscaffold_3G00233120
rosa_rugosa Rorug07G0235700
rosa_samantha Rh7AG382600 Rh7BG369800 Rh7CG402100 Rh7DG381000
rosa_wichuraiana Rw7G032060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 216
AccII CGCG 2 cut(s) 426, 624
AciI CCGC 2 cut(s) 29, 216
AclWI GGATC 3 cut(s) 189, 407, 601
AcsI RAATTY 2 cut(s) 352, 558
AfaI GTAC 1 cut(s) 118
AgsI TTSAA 4 cut(s) 99, 259, 454, 571
AluBI AGCT 4 cut(s) 102, 131, 299, 620
AluI AGCT 4 cut(s) 102, 131, 299, 620
Alw21I GWGCWC 2 cut(s) 538, 622
Alw44I GTGCAC 1 cut(s) 534
AlwI GGATC 3 cut(s) 189, 407, 601
AoxI GGCC 1 cut(s) 600
ApaLI GTGCAC 1 cut(s) 534
ApeKI GCWGC 4 cut(s) 102, 125, 128, 131
ApoI RAATTY 2 cut(s) 352, 558
AspLEI GCGC 2 cut(s) 316, 426
AspS9I GGNCC 2 cut(s) 486, 600
AsuHPI GGTGA 2 cut(s) 358, 487
AvaII GGWCC 1 cut(s) 486
BaeGI GKGCMC 1 cut(s) 538
BaeI ACNNNNGTAYC 2 cut(s) 100, 133
BanII GRGCYC 1 cut(s) 622
BauI CACGAG 1 cut(s) 274
Bbv12I GWGCWC 2 cut(s) 538, 622
BbvI GCAGC 4 cut(s) 89, 112, 118, 140
BccI CCATC 1 cut(s) 540
BclI TGATCA 1 cut(s) 343
BfaI CTAG 2 cut(s) 114, 225
BfmI CTRYAG 2 cut(s) 126, 499
BfoI RGCGCY 1 cut(s) 317
BisI GCNGC 4 cut(s) 103, 126, 129, 132
BlsI GCNGC 4 cut(s) 104, 127, 130, 133
Bme18I GGWCC 1 cut(s) 486
BmgT120I GGNCC 2 cut(s) 486, 600
BplI GAGNNNNNCTC 2 cut(s) 604, 636
BpuEI CTTGAG 1 cut(s) 584
BseGI GGATG 1 cut(s) 325
BseMII CTCAG 1 cut(s) 37
BseRI GAGGAG 1 cut(s) 628
BseSI GKGCMC 1 cut(s) 538
BseXI GCAGC 4 cut(s) 89, 112, 118, 140
Bsh1236I CGCG 2 cut(s) 426, 624
BshFI GGCC 1 cut(s) 602
BsiHKAI GWGCWC 2 cut(s) 538, 622
BsnI GGCC 1 cut(s) 602
Bsp1286I GDGCHC 2 cut(s) 538, 622
Bsp143I GATC 5 cut(s) 194, 288, 343, 412, 593
Bsp68I TCGCGA 1 cut(s) 624
BspACI CCGC 2 cut(s) 29, 216
BspANI GGCC 1 cut(s) 602
BspCNI CTCAG 1 cut(s) 36
BspFNI CGCG 2 cut(s) 426, 624
BspMAI CTGCAG 1 cut(s) 130
BspPI GGATC 3 cut(s) 189, 407, 601
BsrBI CCGCTC 1 cut(s) 216
BssMI GATC 5 cut(s) 194, 288, 343, 412, 593
BssSI CACGAG 1 cut(s) 274
Bst2BI CACGAG 1 cut(s) 274
Bst4CI ACNGT 1 cut(s) 503
Bst6I CTCTTC 1 cut(s) 566
BstC8I GCNNGC 4 cut(s) 169, 297, 604, 622
BstDEI CTNAG 2 cut(s) 23, 251
BstF5I GGATG 1 cut(s) 325
BstFNI CGCG 2 cut(s) 426, 624
BstH2I RGCGCY 1 cut(s) 317
BstHHI GCGC 2 cut(s) 316, 426
BstKTI GATC 5 cut(s) 197, 291, 346, 415, 596
BstMBI GATC 5 cut(s) 194, 288, 343, 412, 593
BstMWI GCNNNNNNNGC 3 cut(s) 131, 173, 379
BstNSI RCATGY 1 cut(s) 192
BstSFI CTRYAG 2 cut(s) 126, 499
BstSLI GKGCMC 1 cut(s) 538
BstUI CGCG 2 cut(s) 426, 624
BstV1I GCAGC 4 cut(s) 89, 112, 118, 140
BstX2I RGATCY 2 cut(s) 194, 412
BstYI RGATCY 2 cut(s) 194, 412
BsuRI GGCC 1 cut(s) 602
BtgZI GCGATG 1 cut(s) 441
BtsCI GGATG 1 cut(s) 325
BtuMI TCGCGA 1 cut(s) 624
Cac8I GCNNGC 4 cut(s) 169, 297, 604, 622
CfoI GCGC 2 cut(s) 316, 426
Cfr13I GGNCC 2 cut(s) 486, 600
CseI GACGC 1 cut(s) 412
Csp6I GTAC 1 cut(s) 117
CviAII CATG 6 cut(s) 45, 73, 164, 189, 310, 597
CviJI RGCY 9 cut(s) 21, 71, 102, 131, 176, 299, 395, 602, 620
CviKI_1 RGCY 9 cut(s) 21, 71, 102, 131, 176, 299, 395, 602, 620
CviQI GTAC 1 cut(s) 117
DdeI CTNAG 2 cut(s) 23, 251
DpnI GATC 5 cut(s) 196, 290, 345, 414, 595
DpnII GATC 5 cut(s) 194, 288, 343, 412, 593
Eam1104I CTCTTC 1 cut(s) 566
EarI CTCTTC 1 cut(s) 566
Ecl136II GAGCTC 1 cut(s) 620
Eco24I GRGCYC 1 cut(s) 622
Eco47I GGWCC 1 cut(s) 486
Eco53kI GAGCTC 1 cut(s) 620
EcoICRI GAGCTC 1 cut(s) 620
EcoRI GAATTC 1 cut(s) 352
EcoT22I ATGCAT 1 cut(s) 50
EcoT38I GRGCYC 1 cut(s) 622
FaeI CATG 6 cut(s) 48, 76, 167, 192, 313, 600
FatI CATG 6 cut(s) 44, 72, 163, 188, 309, 596
FbaI TGATCA 1 cut(s) 343
Fnu4HI GCNGC 4 cut(s) 103, 126, 129, 132
FokI GGATG 1 cut(s) 312
FriOI GRGCYC 1 cut(s) 622
Fsp4HI GCNGC 4 cut(s) 103, 126, 129, 132
FspBI CTAG 2 cut(s) 114, 225
GlaI GCGC 2 cut(s) 315, 425
GluI GCNGC 4 cut(s) 103, 126, 129, 132
HaeII RGCGCY 1 cut(s) 317
HaeIII GGCC 1 cut(s) 602
HgaI GACGC 1 cut(s) 412
HhaI GCGC 2 cut(s) 316, 426
Hin1II CATG 6 cut(s) 48, 76, 167, 192, 313, 600
Hin6I GCGC 2 cut(s) 314, 424
HinP1I GCGC 2 cut(s) 314, 424
HincII GTYRAC 1 cut(s) 554
HindII GTYRAC 1 cut(s) 554
HinfI GANTC 2 cut(s) 259, 550
HphI GGTGA 2 cut(s) 358, 487
Hpy166II GTNNAC 2 cut(s) 536, 554
Hpy188I TCNGA 2 cut(s) 264, 521
Hpy188III TCNNGA 3 cut(s) 329, 563, 623
Hpy8I GTNNAC 2 cut(s) 536, 554
HpyAV CCTTC 4 cut(s) 156, 502, 570, 584
HpyCH4III ACNGT 1 cut(s) 503
HpyCH4V TGCA 8 cut(s) 48, 55, 105, 128, 167, 192, 382, 536
HpyF10VI GCNNNNNNNGC 3 cut(s) 131, 173, 379
HpyF3I CTNAG 2 cut(s) 23, 251
Hsp92II CATG 6 cut(s) 48, 76, 167, 192, 313, 600
HspAI GCGC 2 cut(s) 314, 424
Ksp22I TGATCA 1 cut(s) 343
Kzo9I GATC 5 cut(s) 194, 288, 343, 412, 593
LpnPI CCDG 2 cut(s) 342, 616
Lsp1109I GCAGC 4 cut(s) 89, 112, 118, 140
MaeI CTAG 2 cut(s) 114, 225
MaeIII GTNAC 3 cut(s) 206, 464, 579
MalI GATC 5 cut(s) 196, 290, 345, 414, 595
MbiI CCGCTC 1 cut(s) 216
MboI GATC 5 cut(s) 194, 288, 343, 412, 593
MboII GAAGA 2 cut(s) 87, 583
MflI RGATCY 2 cut(s) 194, 412
MhlI GDGCHC 2 cut(s) 538, 622
MluCI AATT 6 cut(s) 157, 183, 235, 352, 511, 558
MlyI GAGTC 1 cut(s) 559
MnlI CCTC 6 cut(s) 32, 91, 385, 426, 477, 606
Mph1103I ATGCAT 1 cut(s) 50
MslI CAYNNNNRTG 2 cut(s) 71, 330
MspA1I CMGCKG 1 cut(s) 131
MvnI CGCG 2 cut(s) 426, 624
MwoI GCNNNNNNNGC 3 cut(s) 131, 173, 379
NdeII GATC 5 cut(s) 194, 288, 343, 412, 593
NlaIII CATG 6 cut(s) 48, 76, 167, 192, 313, 600
NmuCI GTSAC 1 cut(s) 579
NruI TCGCGA 1 cut(s) 624
NsiI ATGCAT 1 cut(s) 50
NspI RCATGY 1 cut(s) 192
PfeI GAWTC 1 cut(s) 259
PkrI GCNGC 4 cut(s) 104, 127, 130, 133
PleI GAGTC 1 cut(s) 558
PpsI GAGTC 1 cut(s) 558
Psp124BI GAGCTC 1 cut(s) 622
PspPI GGNCC 2 cut(s) 486, 600
PsrI GAACNNNNNNTAC 2 cut(s) 380, 412
PstI CTGCAG 1 cut(s) 130
PsuI RGATCY 2 cut(s) 194, 412
PvuII CAGCTG 1 cut(s) 131
RruI TCGCGA 1 cut(s) 624
RsaI GTAC 1 cut(s) 118
RsaNI GTAC 1 cut(s) 117
RseI CAYNNNNRTG 2 cut(s) 71, 330
SacI GAGCTC 1 cut(s) 622
SatI GCNGC 4 cut(s) 103, 126, 129, 132
Sau3AI GATC 5 cut(s) 194, 288, 343, 412, 593
Sau96I GGNCC 2 cut(s) 486, 600
SchI GAGTC 1 cut(s) 559
SduI GDGCHC 2 cut(s) 538, 622
SfcI CTRYAG 2 cut(s) 126, 499
SinI GGWCC 1 cut(s) 486
SmiMI CAYNNNNRTG 2 cut(s) 71, 330
SmlI CTYRAG 1 cut(s) 563
SmoI CTYRAG 1 cut(s) 563
Sse9I AATT 6 cut(s) 157, 183, 235, 352, 511, 558
SsiI CCGC 2 cut(s) 29, 216
SspI AATATT 1 cut(s) 361
SspMI CTAG 2 cut(s) 114, 225
SstI GAGCTC 1 cut(s) 622
TaaI ACNGT 1 cut(s) 503
TasI AATT 6 cut(s) 157, 183, 235, 352, 511, 558
TfiI GAWTC 1 cut(s) 259
TseFI GTSAC 1 cut(s) 579
TseI GCWGC 4 cut(s) 102, 125, 128, 131
Tsp45I GTSAC 1 cut(s) 579
TspDTI ATGAA 2 cut(s) 89, 444
VneI GTGCAC 1 cut(s) 534
VpaK11BI GGWCC 1 cut(s) 486
XapI RAATTY 2 cut(s) 352, 558
XceI RCATGY 1 cut(s) 192
XspI CTAG 2 cut(s) 114, 225
Zsp2I ATGCAT 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.