RLG00000001758

Chaperone protein dnaJ 20, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0016939
Physical Location & Seq
Forward (+)
20223331 .. 20223915
585 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001758

Sequence Viewer

Length: 510 bp
ATGAATACTACTTGGATGACCACCTCAACCCTAAACTTGGCTGCTAATAAGCTTTCTTTTCTTTCTCATAAATCATCACCAGCTACTATCAGCTATAGTAAGTTTTCTGTTGCATGCAGAGCTACTAATTATGATACAAAGAAGGAGGAAAAAACCAACTACTATAAGTTACTTAGTTTGAGTCCCAATAGTAATACAAGCATGGATGACATAAAGAGAGCTTACAGAAGCATGGCTCTCCGGTACCACCCTGACGTTGTGGATCATCGAGCTGATGATTCTTCGATGAAGGAGTCGACTCAGTTGTTTGTTCAGCTGAATGAGGCATACAAGACGCTGTCGGATCCCGTGTTGCGTGAGGAGAAGGATATGAGTACTGTTGGACCAAATTTGCAATGGCGACATCAGATTCTCGAGTTGAAGAGAAGATCAAACGGCCGCATGGCACGAAAGGAAGGATCCTGGGCTAGCAGAGTCAGGAGGGCAGAACATGTTTCAACCATGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

170

Amino Acids

19.5

Weight (kDa)

9.65

Isoelectric Point (pI)

49.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 53 - 123 4.6e-16 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016939)

Species Orthologous Gene IDs
malus_domestica MD08G1221200.v1.1
prunus_persica Prupe.1G558200_v2.0.a1
pyrus_communis pycom15g36670
rosa_chinensis RchiOBHm_Chr7g0226211
rosa_laevigata RLG00000001758
rosa_multiflora Rmu_sc0001350.1_g000004 Rmu_sc0013267.1_g000006
rosa_roxburghii Rroxscaffold_3G00233120
rosa_rugosa Rorug07G0235700
rosa_samantha Rh7AG382600 Rh7BG369800 Rh7CG402100 Rh7DG381000
rosa_wichuraiana Rw7G032060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 243
AccB1I GGYRCC 1 cut(s) 243
AccI GTMKAC 1 cut(s) 296
AciI CCGC 1 cut(s) 439
AclWI GGATC 5 cut(s) 270, 338, 351, 453, 466
AcoI YGGCCR 1 cut(s) 436
AcsI RAATTY 1 cut(s) 388
AfaI GTAC 2 cut(s) 245, 376
AfiI CCNNNNNNNGG 1 cut(s) 37
AflIII ACRYGT 1 cut(s) 490
AgsI TTSAA 2 cut(s) 421, 498
AjnI CCWGG 1 cut(s) 461
AluBI AGCT 7 cut(s) 52, 83, 93, 122, 221, 272, 316
AluI AGCT 7 cut(s) 52, 83, 93, 122, 221, 272, 316
AlwI GGATC 5 cut(s) 270, 338, 351, 453, 466
Ama87I CYCGRG 1 cut(s) 413
AoxI GGCC 1 cut(s) 436
ApeKI GCWGC 1 cut(s) 41
ApoI RAATTY 1 cut(s) 388
Asp718I GGTACC 1 cut(s) 243
AspS9I GGNCC 1 cut(s) 383
AsuHPI GGTGA 1 cut(s) 69
AsuNHI GCTAGC 1 cut(s) 467
AvaI CYCGRG 1 cut(s) 413
AvaII GGWCC 1 cut(s) 383
BamHI GGATCC 2 cut(s) 343, 458
BanI GGYRCC 1 cut(s) 243
BbvI GCAGC 1 cut(s) 28
BceAI ACGGC 1 cut(s) 451
BciT130I CCWGG 1 cut(s) 463
BfaI CTAG 1 cut(s) 468
BfmI CTRYAG 1 cut(s) 94
BisI GCNGC 2 cut(s) 42, 439
BlsI GCNGC 2 cut(s) 43, 440
BmcAI AGTACT 1 cut(s) 376
Bme1390I CCNGG 1 cut(s) 463
Bme18I GGWCC 1 cut(s) 383
BmeT110I CYCGRG 1 cut(s) 413
BmgT120I GGNCC 1 cut(s) 383
BmiI GGNNCC 3 cut(s) 245, 345, 460
BmrFI CCNGG 1 cut(s) 463
BmtI GCTAGC 1 cut(s) 471
BsaJI CCNNGG 2 cut(s) 462, 501
BsaWI WCCGGW 1 cut(s) 240
Bsc4I CCNNNNNNNGG 1 cut(s) 37
Bse3DI GCAATG 1 cut(s) 401
BseBI CCWGG 1 cut(s) 463
BseDI CCNNGG 2 cut(s) 462, 501
BseGI GGATG 2 cut(s) 21, 211
BseLI CCNNNNNNNGG 1 cut(s) 37
BseMI GCAATG 1 cut(s) 401
BseMII CTCAG 1 cut(s) 314
BseRI GAGGAG 1 cut(s) 374
BseX3I CGGCCG 1 cut(s) 436
BseXI GCAGC 1 cut(s) 28
Bsh1285I CGRYCG 1 cut(s) 439
BshFI GGCC 1 cut(s) 438
BshNI GGYRCC 1 cut(s) 243
BsiEI CGRYCG 1 cut(s) 439
BsiHKCI CYCGRG 1 cut(s) 413
BsiSI CCGG 1 cut(s) 241
BslFI GGGAC 1 cut(s) 168
BslI CCNNNNNNNGG 1 cut(s) 37
BsmFI GGGAC 1 cut(s) 168
BsnI GGCC 1 cut(s) 438
BsoBI CYCGRG 1 cut(s) 413
Bsp143I GATC 4 cut(s) 262, 343, 428, 458
Bsp19I CCATGG 1 cut(s) 501
BspACI CCGC 1 cut(s) 439
BspANI GGCC 1 cut(s) 438
BspCNI CTCAG 1 cut(s) 313
BspLI GGNNCC 3 cut(s) 245, 345, 460
BspOI GCTAGC 1 cut(s) 471
BspPI GGATC 5 cut(s) 270, 338, 351, 453, 466
BspT107I GGYRCC 1 cut(s) 243
BsrDI GCAATG 1 cut(s) 401
BssECI CCNNGG 2 cut(s) 462, 501
BssMI GATC 4 cut(s) 262, 343, 428, 458
BssT1I CCWWGG 1 cut(s) 501
Bst2UI CCWGG 1 cut(s) 463
Bst4CI ACNGT 1 cut(s) 379
Bst6I CTCTTC 1 cut(s) 416
BstC8I GCNNGC 2 cut(s) 115, 469
BstDEI CTNAG 2 cut(s) 173, 300
BstDSI CCRYGG 1 cut(s) 501
BstF5I GGATG 2 cut(s) 21, 211
BstKTI GATC 4 cut(s) 265, 346, 431, 461
BstMBI GATC 4 cut(s) 262, 343, 428, 458
BstMCI CGRYCG 1 cut(s) 439
BstMWI GCNNNNNNNGC 1 cut(s) 119
BstNI CCWGG 1 cut(s) 463
BstNSI RCATGY 2 cut(s) 117, 494
BstSCI CCNGG 1 cut(s) 461
BstSFI CTRYAG 1 cut(s) 94
BstV1I GCAGC 1 cut(s) 28
BstX2I RGATCY 2 cut(s) 343, 458
BstYI RGATCY 2 cut(s) 343, 458
BstZI CGGCCG 1 cut(s) 436
BsuRI GGCC 1 cut(s) 438
BtgI CCRYGG 1 cut(s) 501
BtsCI GGATG 2 cut(s) 21, 211
Cac8I GCNNGC 2 cut(s) 115, 469
Cfr13I GGNCC 1 cut(s) 383
CseI GACGC 1 cut(s) 343
Csp6I GTAC 2 cut(s) 244, 375
CviAII CATG 6 cut(s) 114, 202, 232, 442, 491, 502
CviQI GTAC 2 cut(s) 244, 375
DdeI CTNAG 2 cut(s) 173, 300
DpnI GATC 4 cut(s) 264, 345, 430, 460
DpnII GATC 4 cut(s) 262, 343, 428, 458
EaeI YGGCCR 1 cut(s) 436
EagI CGGCCG 1 cut(s) 436
Eam1104I CTCTTC 1 cut(s) 416
EarI CTCTTC 1 cut(s) 416
EclXI CGGCCG 1 cut(s) 436
Eco130I CCWWGG 1 cut(s) 501
Eco47I GGWCC 1 cut(s) 383
Eco52I CGGCCG 1 cut(s) 436
Eco88I CYCGRG 1 cut(s) 413
EcoRII CCWGG 1 cut(s) 461
EcoT14I CCWWGG 1 cut(s) 501
ErhI CCWWGG 1 cut(s) 501
FaeI CATG 6 cut(s) 117, 205, 235, 445, 494, 505
FaqI GGGAC 1 cut(s) 168
FatI CATG 6 cut(s) 113, 201, 231, 441, 490, 501
FblI GTMKAC 1 cut(s) 296
Fnu4HI GCNGC 2 cut(s) 42, 439
FokI GGATG 2 cut(s) 28, 218
Fsp4HI GCNGC 2 cut(s) 42, 439
FspBI CTAG 1 cut(s) 468
GluI GCNGC 2 cut(s) 42, 439
HaeIII GGCC 1 cut(s) 438
HapII CCGG 1 cut(s) 241
HgaI GACGC 1 cut(s) 343
Hin1II CATG 6 cut(s) 117, 205, 235, 445, 494, 505
HincII GTYRAC 1 cut(s) 297
HindII GTYRAC 1 cut(s) 297
HindIII AAGCTT 1 cut(s) 50
HinfI GANTC 6 cut(s) 181, 278, 293, 298, 409, 474
HpaII CCGG 1 cut(s) 241
HphI GGTGA 1 cut(s) 69
Hpy166II GTNNAC 1 cut(s) 297
Hpy188I TCNGA 2 cut(s) 343, 408
Hpy188III TCNNGA 2 cut(s) 413, 478
Hpy8I GTNNAC 1 cut(s) 297
HpyAV CCTTC 4 cut(s) 136, 283, 358, 449
HpyCH4III ACNGT 1 cut(s) 379
HpyCH4IV ACGT 1 cut(s) 255
HpyCH4V TGCA 3 cut(s) 113, 117, 394
HpyF10VI GCNNNNNNNGC 1 cut(s) 119
HpyF3I CTNAG 2 cut(s) 173, 300
HpySE526I ACGT 1 cut(s) 255
Hsp92II CATG 6 cut(s) 117, 205, 235, 445, 494, 505
KpnI GGTACC 1 cut(s) 247
Kzo9I GATC 4 cut(s) 262, 343, 428, 458
LpnPI CCDG 6 cut(s) 93, 254, 264, 448, 463, 475
Lsp1109I GCAGC 1 cut(s) 28
MaeI CTAG 1 cut(s) 468
MaeII ACGT 1 cut(s) 255
MaeIII GTNAC 1 cut(s) 168
MalI GATC 4 cut(s) 264, 345, 430, 460
MboI GATC 4 cut(s) 262, 343, 428, 458
MboII GAAGA 3 cut(s) 273, 433, 438
MflI RGATCY 2 cut(s) 343, 458
MluCI AATT 2 cut(s) 127, 388
MlyI GAGTC 4 cut(s) 190, 292, 302, 483
MmeI TCCRAC 2 cut(s) 321, 361
MnlI CCTC 5 cut(s) 34, 139, 316, 352, 474
MspA1I CMGCKG 1 cut(s) 316
MspI CCGG 1 cut(s) 241
MspR9I CCNGG 1 cut(s) 463
MvaI CCWGG 1 cut(s) 463
MwoI GCNNNNNNNGC 1 cut(s) 119
NcoI CCATGG 1 cut(s) 501
NdeII GATC 4 cut(s) 262, 343, 428, 458
NheI GCTAGC 1 cut(s) 467
NlaIII CATG 6 cut(s) 117, 205, 235, 445, 494, 505
NlaIV GGNNCC 3 cut(s) 245, 345, 460
NspI RCATGY 2 cut(s) 117, 494
PaeI GCATGC 1 cut(s) 117
PaeR7I CTCGAG 1 cut(s) 413
PciI ACATGT 1 cut(s) 490
PfeI GAWTC 2 cut(s) 278, 409
PflFI GACNNNGTC 1 cut(s) 337
PkrI GCNGC 2 cut(s) 43, 440
PleI GAGTC 4 cut(s) 189, 292, 301, 482
PpsI GAGTC 4 cut(s) 189, 292, 301, 482
PscI ACATGT 1 cut(s) 490
Psp6I CCWGG 1 cut(s) 461
PspGI CCWGG 1 cut(s) 461
PspN4I GGNNCC 3 cut(s) 245, 345, 460
PspPI GGNCC 1 cut(s) 383
PsuI RGATCY 2 cut(s) 343, 458
PsyI GACNNNGTC 1 cut(s) 337
PvuII CAGCTG 1 cut(s) 316
RsaI GTAC 2 cut(s) 245, 376
RsaNI GTAC 2 cut(s) 244, 375
SalI GTCGAC 1 cut(s) 295
SatI GCNGC 2 cut(s) 42, 439
Sau3AI GATC 4 cut(s) 262, 343, 428, 458
Sau96I GGNCC 1 cut(s) 383
ScaI AGTACT 1 cut(s) 376
SchI GAGTC 4 cut(s) 190, 292, 302, 483
ScrFI CCNGG 1 cut(s) 463
SetI ASST 9 cut(s) 26, 54, 85, 95, 124, 223, 258, 274, 318
SfcI CTRYAG 1 cut(s) 94
Sfr274I CTCGAG 1 cut(s) 413
SinI GGWCC 1 cut(s) 383
SlaI CTCGAG 1 cut(s) 413
SmlI CTYRAG 1 cut(s) 413
SmoI CTYRAG 1 cut(s) 413
SphI GCATGC 1 cut(s) 117
Sse9I AATT 2 cut(s) 127, 388
SsiI CCGC 1 cut(s) 439
SspMI CTAG 1 cut(s) 468
StyD4I CCNGG 1 cut(s) 461
StyI CCWWGG 1 cut(s) 501
TaaI ACNGT 1 cut(s) 379
TaiI ACGT 1 cut(s) 258
TaqI TCGA 4 cut(s) 268, 284, 296, 414
TasI AATT 2 cut(s) 127, 388
TatI WGTACW 1 cut(s) 374
TauI GCSGC 1 cut(s) 441
TfiI GAWTC 2 cut(s) 278, 409
TseI GCWGC 1 cut(s) 41
TspDTI ATGAA 2 cut(s) 17, 302
Tth111I GACNNNGTC 1 cut(s) 337
VpaK11BI GGWCC 1 cut(s) 383
XapI RAATTY 1 cut(s) 388
XceI RCATGY 2 cut(s) 117, 494
XcmI CCANNNNNNNNNTGG 1 cut(s) 393
XhoI CTCGAG 1 cut(s) 413
XmiI GTMKAC 1 cut(s) 296
XspI CTAG 1 cut(s) 468
ZrmI AGTACT 1 cut(s) 376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.