RchiOBHm_Chr7g0226211

Chaperone protein dnaJ 20, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0016939
Physical Location & Seq
Reverse (-)
49364621 .. 49365205
585 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20261

Sequence Viewer

Length: 585 bp
ATGAATACTACTTGGGTGACCACCTCAACCCTAAACACTAGCTTGGCTGCTAATACTTTTCTTTCTCATAAATCATCACCAGCTACTATCACTTATAGTAAGTTTTCCGTTGCGTGCAGAGCTACTAATTATGATACAAGAAACGAGAAAAAAACCAACTACTATAAGTTACTTAGTTTGAGTCCCAATAGTAATACAAGCATGGATGACATAAAGAGAGCTTACAGAAGCATGGCTCTCCGGTACCACCCTGACGTTGTGGATCATCGAGCTGATGATTCTTCGATGAAGGAGTCGACTCAGTTGTTTGTTCAGCTGAATGAGGCATACAAGACGCTGTCGGATCCCGTGTTGCGTGAGGAGTACGATTATGAATTGGGTTTGACAGCGAATTCATCATGCAGTTCATCTTGTAGGCGTACTACCTATAAATTCAGGAAGGATATGAGTACCGTTGGACCAAATTTGCAATGGCGACAACAGATTCTCGAGTTGAAGAGAAGATCGAACGGCCGTATGGCACGAAAGGAAGGATCCTGGGCTAGCAGAGTCAGGAGGGCAGAACATGTTTCAACCATGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

194

Amino Acids

22.34

Weight (kDa)

9.62

Isoelectric Point (pI)

51.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 53 - 123 1.3e-17 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016939)

Species Orthologous Gene IDs
malus_domestica MD08G1221200.v1.1
prunus_persica Prupe.1G558200_v2.0.a1
pyrus_communis pycom15g36670
rosa_chinensis RchiOBHm_Chr7g0226211
rosa_laevigata RLG00000001758
rosa_multiflora Rmu_sc0001350.1_g000004 Rmu_sc0013267.1_g000006
rosa_roxburghii Rroxscaffold_3G00233120
rosa_rugosa Rorug07G0235700
rosa_samantha Rh7AG382600 Rh7BG369800 Rh7CG402100 Rh7DG381000
rosa_wichuraiana Rw7G032060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 243
AccB1I GGYRCC 1 cut(s) 243
AccI GTMKAC 1 cut(s) 296
AclWI GGATC 5 cut(s) 270, 338, 351, 528, 541
AcoI YGGCCR 1 cut(s) 511
AcsI RAATTY 3 cut(s) 391, 431, 463
AfaI GTAC 4 cut(s) 245, 365, 421, 451
AflIII ACRYGT 1 cut(s) 565
AgsI TTSAA 2 cut(s) 496, 573
AjnI CCWGG 1 cut(s) 536
AluBI AGCT 6 cut(s) 42, 83, 122, 221, 272, 316
AluI AGCT 6 cut(s) 42, 83, 122, 221, 272, 316
AlwI GGATC 5 cut(s) 270, 338, 351, 528, 541
Ama87I CYCGRG 1 cut(s) 488
AoxI GGCC 1 cut(s) 511
ApeKI GCWGC 1 cut(s) 47
ApoI RAATTY 3 cut(s) 391, 431, 463
Asp718I GGTACC 1 cut(s) 243
AspS9I GGNCC 1 cut(s) 458
AsuHPI GGTGA 2 cut(s) 28, 69
AsuNHI GCTAGC 1 cut(s) 542
AvaI CYCGRG 1 cut(s) 488
AvaII GGWCC 1 cut(s) 458
BamHI GGATCC 2 cut(s) 343, 533
BanI GGYRCC 1 cut(s) 243
BbvI GCAGC 1 cut(s) 34
BceAI ACGGC 2 cut(s) 498, 526
BciT130I CCWGG 1 cut(s) 538
BfaI CTAG 2 cut(s) 39, 543
BisI GCNGC 1 cut(s) 48
BlsI GCNGC 1 cut(s) 49
Bme1390I CCNGG 1 cut(s) 538
Bme18I GGWCC 1 cut(s) 458
BmeT110I CYCGRG 1 cut(s) 488
BmgT120I GGNCC 1 cut(s) 458
BmiI GGNNCC 3 cut(s) 245, 345, 535
BmrFI CCNGG 1 cut(s) 538
BmtI GCTAGC 1 cut(s) 546
BsaJI CCNNGG 2 cut(s) 537, 576
BsaWI WCCGGW 1 cut(s) 240
Bse3DI GCAATG 1 cut(s) 476
BseBI CCWGG 1 cut(s) 538
BseDI CCNNGG 2 cut(s) 537, 576
BseGI GGATG 1 cut(s) 211
BseMI GCAATG 1 cut(s) 476
BseMII CTCAG 1 cut(s) 314
BseRI GAGGAG 1 cut(s) 374
BseX3I CGGCCG 1 cut(s) 511
BseXI GCAGC 1 cut(s) 34
BsgI GTGCAG 1 cut(s) 136
Bsh1285I CGRYCG 1 cut(s) 514
BshFI GGCC 1 cut(s) 513
BshNI GGYRCC 1 cut(s) 243
BsiEI CGRYCG 1 cut(s) 514
BsiHKCI CYCGRG 1 cut(s) 488
BsiSI CCGG 1 cut(s) 241
BslFI GGGAC 1 cut(s) 168
BsmFI GGGAC 1 cut(s) 168
BsnI GGCC 1 cut(s) 513
BsoBI CYCGRG 1 cut(s) 488
Bsp143I GATC 4 cut(s) 262, 343, 503, 533
Bsp19I CCATGG 1 cut(s) 576
BspANI GGCC 1 cut(s) 513
BspCNI CTCAG 1 cut(s) 313
BspLI GGNNCC 3 cut(s) 245, 345, 535
BspOI GCTAGC 1 cut(s) 546
BspPI GGATC 5 cut(s) 270, 338, 351, 528, 541
BspT107I GGYRCC 1 cut(s) 243
BsrDI GCAATG 1 cut(s) 476
BssECI CCNNGG 2 cut(s) 537, 576
BssMI GATC 4 cut(s) 262, 343, 503, 533
BssT1I CCWWGG 1 cut(s) 576
Bst2UI CCWGG 1 cut(s) 538
Bst4CI ACNGT 1 cut(s) 454
Bst6I CTCTTC 1 cut(s) 491
BstC8I GCNNGC 2 cut(s) 115, 544
BstDEI CTNAG 2 cut(s) 173, 300
BstDSI CCRYGG 1 cut(s) 576
BstEII GGTNACC 1 cut(s) 16
BstF5I GGATG 1 cut(s) 211
BstKTI GATC 4 cut(s) 265, 346, 506, 536
BstMBI GATC 4 cut(s) 262, 343, 503, 533
BstMCI CGRYCG 1 cut(s) 514
BstMWI GCNNNNNNNGC 1 cut(s) 119
BstNI CCWGG 1 cut(s) 538
BstNSI RCATGY 1 cut(s) 569
BstPI GGTNACC 1 cut(s) 16
BstSCI CCNGG 1 cut(s) 536
BstV1I GCAGC 1 cut(s) 34
BstX2I RGATCY 2 cut(s) 343, 533
BstYI RGATCY 2 cut(s) 343, 533
BstZI CGGCCG 1 cut(s) 511
BsuRI GGCC 1 cut(s) 513
BtgI CCRYGG 1 cut(s) 576
BtsCI GGATG 1 cut(s) 211
Cac8I GCNNGC 2 cut(s) 115, 544
Cfr13I GGNCC 1 cut(s) 458
CseI GACGC 1 cut(s) 343
Csp6I GTAC 4 cut(s) 244, 364, 420, 450
CviAII CATG 5 cut(s) 202, 232, 399, 566, 577
CviQI GTAC 4 cut(s) 244, 364, 420, 450
DdeI CTNAG 2 cut(s) 173, 300
DpnI GATC 4 cut(s) 264, 345, 505, 535
DpnII GATC 4 cut(s) 262, 343, 503, 533
EaeI YGGCCR 1 cut(s) 511
EagI CGGCCG 1 cut(s) 511
Eam1104I CTCTTC 1 cut(s) 491
EarI CTCTTC 1 cut(s) 491
EclXI CGGCCG 1 cut(s) 511
Eco130I CCWWGG 1 cut(s) 576
Eco47I GGWCC 1 cut(s) 458
Eco52I CGGCCG 1 cut(s) 511
Eco88I CYCGRG 1 cut(s) 488
Eco91I GGTNACC 1 cut(s) 16
EcoO65I GGTNACC 1 cut(s) 16
EcoRI GAATTC 1 cut(s) 391
EcoRII CCWGG 1 cut(s) 536
EcoT14I CCWWGG 1 cut(s) 576
ErhI CCWWGG 1 cut(s) 576
FaeI CATG 5 cut(s) 205, 235, 402, 569, 580
FaqI GGGAC 1 cut(s) 168
FatI CATG 5 cut(s) 201, 231, 398, 565, 576
FblI GTMKAC 1 cut(s) 296
Fnu4HI GCNGC 1 cut(s) 48
FokI GGATG 1 cut(s) 218
Fsp4HI GCNGC 1 cut(s) 48
FspBI CTAG 2 cut(s) 39, 543
GluI GCNGC 1 cut(s) 48
HaeIII GGCC 1 cut(s) 513
HapII CCGG 1 cut(s) 241
HgaI GACGC 1 cut(s) 343
Hin1II CATG 5 cut(s) 205, 235, 402, 569, 580
HincII GTYRAC 1 cut(s) 297
HindII GTYRAC 1 cut(s) 297
HinfI GANTC 6 cut(s) 181, 278, 293, 298, 484, 549
HpaII CCGG 1 cut(s) 241
HphI GGTGA 2 cut(s) 28, 69
Hpy166II GTNNAC 1 cut(s) 297
Hpy188I TCNGA 1 cut(s) 343
Hpy188III TCNNGA 3 cut(s) 436, 488, 553
Hpy8I GTNNAC 1 cut(s) 297
HpyAV CCTTC 3 cut(s) 283, 433, 524
HpyCH4III ACNGT 1 cut(s) 454
HpyCH4IV ACGT 1 cut(s) 255
HpyCH4V TGCA 3 cut(s) 117, 402, 469
HpyF10VI GCNNNNNNNGC 1 cut(s) 119
HpyF3I CTNAG 2 cut(s) 173, 300
HpySE526I ACGT 1 cut(s) 255
Hsp92II CATG 5 cut(s) 205, 235, 402, 569, 580
KpnI GGTACC 1 cut(s) 247
Kzo9I GATC 4 cut(s) 262, 343, 503, 533
LpnPI CCDG 7 cut(s) 93, 254, 264, 421, 523, 538, 550
Lsp1109I GCAGC 1 cut(s) 34
MaeI CTAG 2 cut(s) 39, 543
MaeII ACGT 1 cut(s) 255
MaeIII GTNAC 2 cut(s) 16, 168
MalI GATC 4 cut(s) 264, 345, 505, 535
MboI GATC 4 cut(s) 262, 343, 503, 533
MboII GAAGA 3 cut(s) 273, 508, 513
MflI RGATCY 2 cut(s) 343, 533
MluCI AATT 5 cut(s) 127, 374, 391, 431, 463
MlyI GAGTC 4 cut(s) 190, 292, 302, 558
MmeI TCCRAC 2 cut(s) 321, 436
MnlI CCTC 4 cut(s) 34, 316, 352, 549
MspA1I CMGCKG 1 cut(s) 316
MspI CCGG 1 cut(s) 241
MspR9I CCNGG 1 cut(s) 538
MvaI CCWGG 1 cut(s) 538
MwoI GCNNNNNNNGC 1 cut(s) 119
NcoI CCATGG 1 cut(s) 576
NdeII GATC 4 cut(s) 262, 343, 503, 533
NheI GCTAGC 1 cut(s) 542
NlaIII CATG 5 cut(s) 205, 235, 402, 569, 580
NlaIV GGNNCC 3 cut(s) 245, 345, 535
NmuCI GTSAC 1 cut(s) 16
NspI RCATGY 1 cut(s) 569
PaeR7I CTCGAG 1 cut(s) 488
PciI ACATGT 1 cut(s) 565
PfeI GAWTC 2 cut(s) 278, 484
PflFI GACNNNGTC 1 cut(s) 337
PkrI GCNGC 1 cut(s) 49
PleI GAGTC 4 cut(s) 189, 292, 301, 557
PpsI GAGTC 4 cut(s) 189, 292, 301, 557
PscI ACATGT 1 cut(s) 565
Psp6I CCWGG 1 cut(s) 536
PspEI GGTNACC 1 cut(s) 16
PspGI CCWGG 1 cut(s) 536
PspN4I GGNNCC 3 cut(s) 245, 345, 535
PspPI GGNCC 1 cut(s) 458
PsuI RGATCY 2 cut(s) 343, 533
PsyI GACNNNGTC 1 cut(s) 337
PvuII CAGCTG 1 cut(s) 316
RsaI GTAC 4 cut(s) 245, 365, 421, 451
RsaNI GTAC 4 cut(s) 244, 364, 420, 450
SalI GTCGAC 1 cut(s) 295
SatI GCNGC 1 cut(s) 48
Sau3AI GATC 4 cut(s) 262, 343, 503, 533
Sau96I GGNCC 1 cut(s) 458
SchI GAGTC 4 cut(s) 190, 292, 302, 558
ScrFI CCNGG 1 cut(s) 538
SetI ASST 9 cut(s) 26, 44, 85, 124, 223, 258, 274, 318, 428
Sfr274I CTCGAG 1 cut(s) 488
SinI GGWCC 1 cut(s) 458
SlaI CTCGAG 1 cut(s) 488
SmlI CTYRAG 1 cut(s) 488
SmoI CTYRAG 1 cut(s) 488
Sse9I AATT 5 cut(s) 127, 374, 391, 431, 463
SspMI CTAG 2 cut(s) 39, 543
StyD4I CCNGG 1 cut(s) 536
StyI CCWWGG 1 cut(s) 576
TaaI ACNGT 1 cut(s) 454
TaiI ACGT 1 cut(s) 258
TaqI TCGA 5 cut(s) 268, 284, 296, 489, 506
TasI AATT 5 cut(s) 127, 374, 391, 431, 463
TfiI GAWTC 2 cut(s) 278, 484
TseFI GTSAC 1 cut(s) 16
TseI GCWGC 1 cut(s) 47
Tsp45I GTSAC 1 cut(s) 16
TspDTI ATGAA 5 cut(s) 17, 302, 384, 387, 396
TspGWI ACGGA 1 cut(s) 97
Tth111I GACNNNGTC 1 cut(s) 337
VpaK11BI GGWCC 1 cut(s) 458
XapI RAATTY 3 cut(s) 391, 431, 463
XceI RCATGY 1 cut(s) 569
XcmI CCANNNNNNNNNTGG 1 cut(s) 468
XhoI CTCGAG 1 cut(s) 488
XmiI GTMKAC 1 cut(s) 296
XspI CTAG 2 cut(s) 39, 543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.