Rroxscaffold_3G00233120

Chaperone protein dnaJ 20, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0016939
Physical Location & Seq
Forward (+)
18987206 .. 18987796
591 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00233120.1

Sequence Viewer

Length: 591 bp
ATGAATACTACTTGGATGACCACCTCAAACCTAAACACCAGCTCGGCTGCTAATAAGCTTTCTGTTCTTTGTCATAAATCATCACCAGCTACTATCACTTATAGTAAGTTTTCTGTTGCGTGCAGAGCTACTAATTATGATACACGAAACGAGAAAAAAACCAACTACTATAAGTTACTTAGTTTGAGTCCCAATAGTAATACAAGCATGGATGACATAAAGAGAGCTTACAGAAGCATGGCTCTCCGGTACCACCCTGACGTTGTGGATCATCGAGCTGCTGATTCTTCGATGAAGGAGTCGACTCAGTTGTTTGTTCTGCTGAATGAGGCATACAAGACGCTGTCGGATCCCGTGTTGCGTGAGGAGTACGACTATGAATTGGGTTTGACAGCGAATTCATCATGCAGTTCATCTTGTAGGAGTACTAACTATAAATTCAGGAAGGATATGAGTACTGTTGGACCAAATTTGCAATGGCGACAACAGATTCTCGAGTTGAAGAGAAGATCAACCGGCCGTATGGCACGAAAGGAAGGATCCTGGGCTAGCAGAGTCAGGAGGGCAGAACATGTTTCAACCATGGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

196

Amino Acids

22.41

Weight (kDa)

9.62

Isoelectric Point (pI)

55.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 55 - 125 3.8e-17 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016939)

Species Orthologous Gene IDs
malus_domestica MD08G1221200.v1.1
prunus_persica Prupe.1G558200_v2.0.a1
pyrus_communis pycom15g36670
rosa_chinensis RchiOBHm_Chr7g0226211
rosa_laevigata RLG00000001758
rosa_multiflora Rmu_sc0001350.1_g000004 Rmu_sc0013267.1_g000006
rosa_roxburghii Rroxscaffold_3G00233120
rosa_rugosa Rorug07G0235700
rosa_samantha Rh7AG382600 Rh7BG369800 Rh7CG402100 Rh7DG381000
rosa_wichuraiana Rw7G032060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 249
AccB1I GGYRCC 1 cut(s) 249
AccI GTMKAC 1 cut(s) 302
AclWI GGATC 5 cut(s) 276, 344, 357, 534, 547
AcoI YGGCCR 1 cut(s) 517
AcsI RAATTY 3 cut(s) 397, 437, 469
AfaI GTAC 4 cut(s) 251, 371, 427, 457
AflIII ACRYGT 1 cut(s) 571
AgsI TTSAA 2 cut(s) 502, 579
AjnI CCWGG 1 cut(s) 542
AluBI AGCT 6 cut(s) 42, 58, 89, 128, 227, 278
AluI AGCT 6 cut(s) 42, 58, 89, 128, 227, 278
AlwI GGATC 5 cut(s) 276, 344, 357, 534, 547
Ama87I CYCGRG 1 cut(s) 494
AoxI GGCC 1 cut(s) 517
ApeKI GCWGC 2 cut(s) 47, 278
ApoI RAATTY 3 cut(s) 397, 437, 469
Asp718I GGTACC 1 cut(s) 249
AspS9I GGNCC 1 cut(s) 464
AsuHPI GGTGA 1 cut(s) 75
AsuNHI GCTAGC 1 cut(s) 548
AvaI CYCGRG 1 cut(s) 494
AvaII GGWCC 1 cut(s) 464
BamHI GGATCC 2 cut(s) 349, 539
BanI GGYRCC 1 cut(s) 249
BbvI GCAGC 2 cut(s) 34, 265
BceAI ACGGC 1 cut(s) 504
BciT130I CCWGG 1 cut(s) 544
BfaI CTAG 2 cut(s) 549, 589
BisI GCNGC 2 cut(s) 48, 279
BlsI GCNGC 2 cut(s) 49, 280
BmcAI AGTACT 2 cut(s) 427, 457
Bme1390I CCNGG 1 cut(s) 544
Bme18I GGWCC 1 cut(s) 464
BmeT110I CYCGRG 1 cut(s) 494
BmgT120I GGNCC 1 cut(s) 464
BmiI GGNNCC 3 cut(s) 251, 351, 541
BmrFI CCNGG 1 cut(s) 544
BmtI GCTAGC 1 cut(s) 552
BsaJI CCNNGG 2 cut(s) 543, 582
BsaWI WCCGGW 1 cut(s) 246
Bse118I RCCGGY 1 cut(s) 515
Bse3DI GCAATG 1 cut(s) 482
BseBI CCWGG 1 cut(s) 544
BseDI CCNNGG 2 cut(s) 543, 582
BseGI GGATG 2 cut(s) 21, 217
BseMI GCAATG 1 cut(s) 482
BseMII CTCAG 1 cut(s) 320
BseRI GAGGAG 1 cut(s) 380
BseX3I CGGCCG 1 cut(s) 517
BseXI GCAGC 2 cut(s) 34, 265
BsgI GTGCAG 1 cut(s) 142
Bsh1285I CGRYCG 1 cut(s) 520
BshFI GGCC 1 cut(s) 519
BshNI GGYRCC 1 cut(s) 249
BsiEI CGRYCG 1 cut(s) 520
BsiHKCI CYCGRG 1 cut(s) 494
BsiSI CCGG 2 cut(s) 247, 516
BslFI GGGAC 1 cut(s) 174
BsmFI GGGAC 1 cut(s) 174
BsnI GGCC 1 cut(s) 519
BsoBI CYCGRG 1 cut(s) 494
Bsp143I GATC 4 cut(s) 268, 349, 509, 539
Bsp19I CCATGG 1 cut(s) 582
BspANI GGCC 1 cut(s) 519
BspCNI CTCAG 1 cut(s) 319
BspLI GGNNCC 3 cut(s) 251, 351, 541
BspOI GCTAGC 1 cut(s) 552
BspPI GGATC 5 cut(s) 276, 344, 357, 534, 547
BspT107I GGYRCC 1 cut(s) 249
BsrDI GCAATG 1 cut(s) 482
BsrFI RCCGGY 1 cut(s) 515
BssAI RCCGGY 1 cut(s) 515
BssECI CCNNGG 2 cut(s) 543, 582
BssMI GATC 4 cut(s) 268, 349, 509, 539
BssT1I CCWWGG 1 cut(s) 582
Bst2UI CCWGG 1 cut(s) 544
Bst4CI ACNGT 1 cut(s) 460
Bst6I CTCTTC 1 cut(s) 497
BstC8I GCNNGC 2 cut(s) 121, 550
BstDEI CTNAG 2 cut(s) 179, 306
BstDSI CCRYGG 1 cut(s) 582
BstF5I GGATG 2 cut(s) 21, 217
BstKTI GATC 4 cut(s) 271, 352, 512, 542
BstMBI GATC 4 cut(s) 268, 349, 509, 539
BstMCI CGRYCG 1 cut(s) 520
BstMWI GCNNNNNNNGC 1 cut(s) 125
BstNI CCWGG 1 cut(s) 544
BstNSI RCATGY 1 cut(s) 575
BstSCI CCNGG 1 cut(s) 542
BstV1I GCAGC 2 cut(s) 34, 265
BstX2I RGATCY 2 cut(s) 349, 539
BstYI RGATCY 2 cut(s) 349, 539
BstZI CGGCCG 1 cut(s) 517
BsuRI GGCC 1 cut(s) 519
BtgI CCRYGG 1 cut(s) 582
BtsCI GGATG 2 cut(s) 21, 217
Cac8I GCNNGC 2 cut(s) 121, 550
Cfr10I RCCGGY 1 cut(s) 515
Cfr13I GGNCC 1 cut(s) 464
CseI GACGC 1 cut(s) 349
Csp6I GTAC 4 cut(s) 250, 370, 426, 456
CviAII CATG 5 cut(s) 208, 238, 405, 572, 583
CviQI GTAC 4 cut(s) 250, 370, 426, 456
DdeI CTNAG 2 cut(s) 179, 306
DpnI GATC 4 cut(s) 270, 351, 511, 541
DpnII GATC 4 cut(s) 268, 349, 509, 539
EaeI YGGCCR 1 cut(s) 517
EagI CGGCCG 1 cut(s) 517
Eam1104I CTCTTC 1 cut(s) 497
EarI CTCTTC 1 cut(s) 497
EclXI CGGCCG 1 cut(s) 517
Eco130I CCWWGG 1 cut(s) 582
Eco47I GGWCC 1 cut(s) 464
Eco52I CGGCCG 1 cut(s) 517
Eco88I CYCGRG 1 cut(s) 494
EcoRI GAATTC 1 cut(s) 397
EcoRII CCWGG 1 cut(s) 542
EcoT14I CCWWGG 1 cut(s) 582
ErhI CCWWGG 1 cut(s) 582
FaeI CATG 5 cut(s) 211, 241, 408, 575, 586
FaqI GGGAC 1 cut(s) 174
FatI CATG 5 cut(s) 207, 237, 404, 571, 582
FblI GTMKAC 1 cut(s) 302
Fnu4HI GCNGC 2 cut(s) 48, 279
FokI GGATG 2 cut(s) 28, 224
Fsp4HI GCNGC 2 cut(s) 48, 279
FspBI CTAG 2 cut(s) 549, 589
GluI GCNGC 2 cut(s) 48, 279
HaeIII GGCC 1 cut(s) 519
HapII CCGG 2 cut(s) 247, 516
HgaI GACGC 1 cut(s) 349
Hin1II CATG 5 cut(s) 211, 241, 408, 575, 586
HincII GTYRAC 1 cut(s) 303
HindII GTYRAC 1 cut(s) 303
HindIII AAGCTT 1 cut(s) 56
HinfI GANTC 6 cut(s) 187, 284, 299, 304, 490, 555
HpaII CCGG 2 cut(s) 247, 516
HphI GGTGA 1 cut(s) 75
Hpy166II GTNNAC 1 cut(s) 303
Hpy188I TCNGA 1 cut(s) 349
Hpy188III TCNNGA 3 cut(s) 442, 494, 559
Hpy8I GTNNAC 1 cut(s) 303
HpyAV CCTTC 3 cut(s) 289, 439, 530
HpyCH4III ACNGT 1 cut(s) 460
HpyCH4IV ACGT 1 cut(s) 261
HpyCH4V TGCA 3 cut(s) 123, 408, 475
HpyF10VI GCNNNNNNNGC 1 cut(s) 125
HpyF3I CTNAG 2 cut(s) 179, 306
HpySE526I ACGT 1 cut(s) 261
Hsp92II CATG 5 cut(s) 211, 241, 408, 575, 586
KpnI GGTACC 1 cut(s) 253
Kzo9I GATC 4 cut(s) 268, 349, 509, 539
LpnPI CCDG 9 cut(s) 52, 99, 260, 270, 427, 529, 529, 544, 556
Lsp1109I GCAGC 2 cut(s) 34, 265
MaeI CTAG 2 cut(s) 549, 589
MaeII ACGT 1 cut(s) 261
MaeIII GTNAC 1 cut(s) 174
MalI GATC 4 cut(s) 270, 351, 511, 541
MboI GATC 4 cut(s) 268, 349, 509, 539
MboII GAAGA 3 cut(s) 279, 514, 519
MflI RGATCY 2 cut(s) 349, 539
MluCI AATT 5 cut(s) 133, 380, 397, 437, 469
MlyI GAGTC 4 cut(s) 196, 298, 308, 564
MmeI TCCRAC 2 cut(s) 327, 442
MnlI CCTC 4 cut(s) 34, 322, 358, 555
MspI CCGG 2 cut(s) 247, 516
MspR9I CCNGG 1 cut(s) 544
MvaI CCWGG 1 cut(s) 544
MwoI GCNNNNNNNGC 1 cut(s) 125
NcoI CCATGG 1 cut(s) 582
NdeII GATC 4 cut(s) 268, 349, 509, 539
NheI GCTAGC 1 cut(s) 548
NlaIII CATG 5 cut(s) 211, 241, 408, 575, 586
NlaIV GGNNCC 3 cut(s) 251, 351, 541
NmeAIII GCCGAG 1 cut(s) 23
NspI RCATGY 1 cut(s) 575
PaeR7I CTCGAG 1 cut(s) 494
PciI ACATGT 1 cut(s) 571
PfeI GAWTC 2 cut(s) 284, 490
PflFI GACNNNGTC 1 cut(s) 343
PkrI GCNGC 2 cut(s) 49, 280
PleI GAGTC 4 cut(s) 195, 298, 307, 563
PpsI GAGTC 4 cut(s) 195, 298, 307, 563
PscI ACATGT 1 cut(s) 571
Psp6I CCWGG 1 cut(s) 542
PspGI CCWGG 1 cut(s) 542
PspN4I GGNNCC 3 cut(s) 251, 351, 541
PspPI GGNCC 1 cut(s) 464
PsuI RGATCY 2 cut(s) 349, 539
PsyI GACNNNGTC 1 cut(s) 343
RsaI GTAC 4 cut(s) 251, 371, 427, 457
RsaNI GTAC 4 cut(s) 250, 370, 426, 456
SalI GTCGAC 1 cut(s) 301
SatI GCNGC 2 cut(s) 48, 279
Sau3AI GATC 4 cut(s) 268, 349, 509, 539
Sau96I GGNCC 1 cut(s) 464
ScaI AGTACT 2 cut(s) 427, 457
SchI GAGTC 4 cut(s) 196, 298, 308, 564
ScrFI CCNGG 1 cut(s) 544
SetI ASST 9 cut(s) 26, 33, 44, 60, 91, 130, 229, 264, 280
Sfr274I CTCGAG 1 cut(s) 494
SinI GGWCC 1 cut(s) 464
SlaI CTCGAG 1 cut(s) 494
SmlI CTYRAG 1 cut(s) 494
SmoI CTYRAG 1 cut(s) 494
Sse9I AATT 5 cut(s) 133, 380, 397, 437, 469
SspMI CTAG 2 cut(s) 549, 589
StyD4I CCNGG 1 cut(s) 542
StyI CCWWGG 1 cut(s) 582
TaaI ACNGT 1 cut(s) 460
TaiI ACGT 1 cut(s) 264
TaqI TCGA 4 cut(s) 274, 290, 302, 495
TasI AATT 5 cut(s) 133, 380, 397, 437, 469
TatI WGTACW 2 cut(s) 425, 455
TfiI GAWTC 2 cut(s) 284, 490
TseI GCWGC 2 cut(s) 47, 278
TspDTI ATGAA 5 cut(s) 17, 308, 390, 393, 402
Tth111I GACNNNGTC 1 cut(s) 343
VpaK11BI GGWCC 1 cut(s) 464
XapI RAATTY 3 cut(s) 397, 437, 469
XceI RCATGY 1 cut(s) 575
XcmI CCANNNNNNNNNTGG 1 cut(s) 474
XhoI CTCGAG 1 cut(s) 494
XmiI GTMKAC 1 cut(s) 302
XspI CTAG 2 cut(s) 549, 589
ZrmI AGTACT 2 cut(s) 427, 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.