pycom16g00190
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0005277
Physical Location & Seq
Reverse (-)
104253 .. 111794
7542 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2985 bp
ATGCAACTTAGTTACCTAAATGAGCCATCAGTGTTGTACAATCTTCAGTATAGATACAATCAAGATATGATTTATACAAAAGCAGGACCAGTGTTGGTTGCTGTCAATCCCTTTAAGAGAGTTTCTTTATATGGGAATGAATACATTGAAGCATACAAGCGTAAAGCCGTTGAAAATCCACATGTGTATGCCATTGCAGACACAGCCATTCGGGAAATGGTTCGAGATGAAGTAAATCAGTCTATAATCATAAGTGGGGAAAGTGGAGCAGGAAAAACTGAAACTGCAAAGATAGCAATGCAATATCTGGCTGCACTTGGAGGTGGAAGTGGAATAGAGCATGAAATTTTGAAAACTAATCCTATACTGGAAGCCTTTGGTAATGCGAAAACATTGAGAAATGACAACTCAAGTCGCTTTGGAAAATTGATTGAAATCCACTTCAGTGAAACTGGAAAAATTTCTGGTGCCAATATTCAAACTTTTTTGCTTGAAAAGTCTAGAGTGGTCCAATGCACTGAAGGAGAAAGGTCCTATCATATATTTTATCAGCTTTGTGCTGGAGCTCCTCCTGCTCTTAGAGAGATGCTGAACTTGAAGAGTGCAGATGAGTATCAATATCTGAAGCAAAGCAATTGTTATTCCATTACTGGGGTTAATGATGCTGAAGAATTCAGTGTGGTGAAGGAAGCTCTAGATGTTGTCCATATAAACAAGGAAGACCAACAAAGTGTATTTGCAATGCTTGCTGCAGTTTTGTGGCTGGGAAACATCTCATTTAGTGTGATTGATAACGAAAATCATGTTGAACCTGTAGAAGATGAAGGTCTGTTCAATGTCGCAAAATTGATTGGATGTGGTGTGGATGAACTTAAGCTTGCTTTATCGACTCGAAAAATGAGAGTTGGTAATGACAGTATCGTCCAGAAGCTAACGCAATCTCAGGCCATCGACACAAGAGATGCTTTGTCAAAGTCAATTTATGCATGTTTGTTTGAGTGGTTGGTTGAACAAATTAACAAATCACTTGCAGTAGGAAAACGGCGTAATGGCAGATCTATCAGCATTCTTGATATCTATGGTTTTGAATCATTTGATAGAAATAGTTTTGAACAGTTCTGCATTAATTATGCAAATGAGAGATTGCAACAACACTTCAATCGTCACTTATTCAAGTTAGAGCAGGATGAATATATCCAAGACGGAATTGATTGGACAAAAGTTGAATTTGAAGACAACCAAGATTGTCTTGGTCTTTTCGAAAAGAGACCGTTAGGATTACTGTCTCTGTTAGATGAGGAGTCCACCTTCCCAAATGGTACTGATTTGACCTTTGCCAACAAGCTCAAGCAACATCTGAGCTCTAATTCTTGTTTCAAAGGAGAACGAGGCAAATTGTTTGCTGTCAGTCATTATGCAGGAGAGGTTTCATATGACACAACAGGATTTTTGGAAAAGAACAGGGACTTGCTGCATTTAGATTCTATTCAGCTTCTTTCCTCATGCTCATGCCACCTTCCTCAGATATTTGCATCTAGTATGCTTAATCGACCTGAGAAGCCTTTAGTTGGTCCCTTGTATAAAGTAGGTGGTGGAGTAGACTCCCAGAAGCTGAGTGTTGCAACAAAATTTAAGAGTCAATTGTTCCTATTGATGAAACGTCTAGAAAACACCACTCCACATTTCATACGTTGTATCAAGCCCAACAATCTTCAATCCCCTGGACTATATGAGCAAGGACTTGTACTGCAGCAGCTGAGATGTTGTGGAGTTCTAGAAGTGGTTCGAATATCAAGATCTGGCTTTCCTACCAGAATGTCACACCAGAAGTTTGCAAGAAGATATGGTTTTCTTCTGCTGGAGAATGTTGCATCGCAGGATCCACTTAGTGTTTCAGTTGCTATTCTTCATCAGTTCAACATATTACCAGAGGTGTATCAAGTGGGCTATACAAAGTTATTTTTCCGAACTGGACAGATTGGGGTGCTTGAGGACACCAGAAACCGTACACTCCATGGTATTTTACGTGTTCAAAGCTGCTTTAGAGGACACCAAGCTCGGTGTTACCTCAAGGAGCTGAGGAGAGGAATCACCACTCTCCAGTCATTTATCAGGGGACAGAAAACCAGGAAGGAGTACTCAATATTACTAGAGAGGCATAGAGCTGCTGTTGTCATACAAAAGCAGGTGAAAAGCAGGTTTGCAAGGAAGAAATTCAACAACATTTATGATGCATCAATCGTGATACAATCAGTTCTTCGTGGCTGGTTGGTCAGAAGATGCTCAGGAAGTATAGGATTGTTGAAGCCCGGAAGCATGAAGGCTAACGAGTCAGATGATGTGCTGGTAAAGGCATCTTTCCTTGCTGAATTACAGCGCCGCGTTCTTAAGGCTGAAGCTGGCCTGCGAGAGAAGGAAGAGGAGAATGACATCCTCCACCAACGTCTCCAACAGTATGAGAGCCGTTGGTCGGAATATGAGCAGAAAATGAAATCCATGGAAGAAGTATGGCAGAAACAAATGAGATCCCTACAATCCAGCCTCTCAATTGCAAAGAAGAGCTTAGCCATTGATGATTCCGAGAGAAATTCTGATGCATCAGTGAATGCAAGTGATGACCGTGAGTACAGTTGGGATACAGGAAGTAACCATAGAGGCCAAGATAGCAATGGGGTGAGACCAATGAGTGCAGGTTTGAGCGTCATAAGCCGGTTAACTGAAGAGTTTGATCAGAGGAGTCAAGTTTTTGGGGACGATGCCAAGTTCTTGGTGGAGGTAAAGTCTGGTCAAGTGGAAGCGAGTCTGAATCCAGACCGAGAGCTAAGAACGTTGAAGCAGATGTTTGAGGCTTGGAAGAAGGATTACGGGGCAAGACTAAGGGAGACAAAGGTGATTTTGCATAAGATTGGAAATGAAGAGGGAGGCAGTGCTGACAGGGTGAAAAAGAAGTGGTGGGGAAGGCGGAACAGCTCGAGGATTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000146 GO:0000166 GO:0000278 GO:0000281 GO:0000302 GO:0000910 GO:0001891 GO:0001931 GO:0003674 GO:0003774 GO:0003779 GO:0003824 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005826 GO:0005829 GO:0005856 GO:0005886 GO:0005938 GO:0006928 GO:0006935 GO:0006950 GO:0006970 GO:0006971 GO:0006979 GO:0006996 GO:0007010 GO:0007015 GO:0007033 GO:0007049 GO:0007154 GO:0008064 GO:0008092 GO:0008104 GO:0008144 GO:0008150 GO:0008360 GO:0009267 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009636 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010243 GO:0010639 GO:0014070 GO:0014074 GO:0015629 GO:0016020 GO:0016043 GO:0016050 GO:0016459 GO:0016460 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0022402 GO:0022603 GO:0022604 GO:0022607 GO:0030029 GO:0030030 GO:0030031 GO:0030036 GO:0030038 GO:0030048 GO:0030139 GO:0030554 GO:0030587 GO:0030832 GO:0030833 GO:0030837 GO:0030863 GO:0030864 GO:0030865 GO:0030866 GO:0030898 GO:0031033 GO:0031034 GO:0031152 GO:0031154 GO:0031254 GO:0031268 GO:0031270 GO:0031333 GO:0031410 GO:0031667 GO:0031668 GO:0031669 GO:0031982 GO:0032009 GO:0032060 GO:0032153 GO:0032155 GO:0032271 GO:0032272 GO:0032502 GO:0032535 GO:0032553 GO:0032555 GO:0032559 GO:0032796 GO:0032956 GO:0032970 GO:0032982 GO:0032991 GO:0033036 GO:0033043 GO:0033275 GO:0033298 GO:0033554 GO:0034461 GO:0034622 GO:0035639 GO:0036094 GO:0040011 GO:0042221 GO:0042330 GO:0042493 GO:0042542 GO:0042594 GO:0042623 GO:0042641 GO:0042802 GO:0042803 GO:0042995 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043232 GO:0043254 GO:0043933 GO:0044085 GO:0044087 GO:0044422 GO:0044424 GO:0044425 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044459 GO:0044464 GO:0044764 GO:0044877 GO:0045177 GO:0045179 GO:0045335 GO:0046677 GO:0046683 GO:0046847 GO:0046983 GO:0048519 GO:0048523 GO:0048856 GO:0048870 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0050982 GO:0051015 GO:0051017 GO:0051128 GO:0051129 GO:0051179 GO:0051301 GO:0051493 GO:0051494 GO:0051591 GO:0051606 GO:0051674 GO:0051703 GO:0051704 GO:0051716 GO:0060327 GO:0060328 GO:0061572 GO:0061640 GO:0065003 GO:0065007 GO:0065008 GO:0070252 GO:0070938 GO:0071496 GO:0071840 GO:0071889 GO:0071944 GO:0090066 GO:0090702 GO:0097159 GO:0097204 GO:0097367 GO:0097435 GO:0097708 GO:0098630 GO:0098743 GO:0099120 GO:0099568 GO:0099738 GO:0110053 GO:0120025 GO:0120031 GO:0120036 GO:1901265 GO:1901363 GO:1901698 GO:1901700 GO:1902903 GO:1902904 GO:1903047 GO:1990753
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IQ Myosin_head

Protein Analysis

995

Amino Acids

113.02

Weight (kDa)

8.8

Isoelectric Point (pI)

48.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myosin_head PF00063 2 - 656 8e-241 Myosin head (motor domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2176
AasI GACNNNNNNGTC 1 cut(s) 920
Acc36I ACCTGC 3 cut(s) 2176, 2187, 2681
AccB1I GGYRCC 1 cut(s) 467
AccI GTMKAC 1 cut(s) 1599
AccII CGCG 1 cut(s) 2382
AciI CCGC 2 cut(s) 2380, 2962
AclI AACGTT 1 cut(s) 2828
AclWI GGATC 3 cut(s) 1874, 1887, 2520
AcsI RAATTY 7 cut(s) 345, 459, 671, 1226, 1628, 2213, 2587
AcuI CTGAAG 7 cut(s) 29, 427, 540, 644, 687, 2415, 2739
AdeI CACNNNGTG 1 cut(s) 1889
AfaI GTAC 6 cut(s) 38, 1321, 1746, 2008, 2138, 2627
AfiI CCNNNNNNNGG 3 cut(s) 651, 1568, 2470
AflII CTTAAG 2 cut(s) 872, 2387
AflIII ACRYGT 2 cut(s) 181, 2026
AjnI CCWGG 2 cut(s) 1720, 2126
AleI CACNNNNGTG 1 cut(s) 444
Alw21I GWGCWC 2 cut(s) 568, 1364
Alw26I GTCTC 5 cut(s) 1261, 1290, 2450, 2671, 2876
AlwI GGATC 3 cut(s) 1874, 1887, 2520
AlwNI CAGNNNCTG 2 cut(s) 1612, 1756
Ama87I CYCGRG 1 cut(s) 2971
AoxI GGCC 3 cut(s) 945, 2401, 2656
ApeKI GCWGC 7 cut(s) 311, 749, 1471, 1750, 1753, 2037, 2165
ApoI RAATTY 7 cut(s) 345, 459, 671, 1226, 1628, 2213, 2587
AseI ATTAAT 2 cut(s) 1125, 2979
Asp700I GAANNNNTTC 4 cut(s) 219, 460, 1782, 2213
AspLEI GCGC 1 cut(s) 2379
AspS9I GGNCC 4 cut(s) 86, 508, 531, 1571
AsuC2I CCSGG 1 cut(s) 2310
AsuHPI GGTGA 6 cut(s) 694, 2083, 2200, 2686, 2902, 2950
AsuII TTCGAA 2 cut(s) 1260, 1786
AvaI CYCGRG 1 cut(s) 2971
AvaII GGWCC 4 cut(s) 86, 508, 531, 1571
BamHI GGATCC 1 cut(s) 1879
BanI GGYRCC 1 cut(s) 467
BanII GRGCYC 2 cut(s) 568, 1364
BarI GAAGNNNNNNTAC 2 cut(s) 2846, 2878
BbsI GAAGAC 2 cut(s) 726, 1239
Bbv12I GWGCWC 2 cut(s) 568, 1364
BbvCI CCTCAGC 1 cut(s) 2078
BbvI GCAGC 7 cut(s) 298, 736, 1458, 1762, 1765, 2024, 2152
BccI CCATC 2 cut(s) 34, 956
BceAI ACGGC 3 cut(s) 152, 1058, 2448
BciT130I CCWGG 2 cut(s) 1722, 2128
BciVI GTATCC 1 cut(s) 2629
BclI TGATCA 1 cut(s) 2728
BcnI CCSGG 1 cut(s) 2310
BcoDI GTCTC 5 cut(s) 1261, 1290, 2450, 2671, 2876
BfaI CTAG 6 cut(s) 501, 695, 1536, 1664, 1775, 2150
BfmI CTRYAG 3 cut(s) 750, 813, 1748
BfoI RGCGCY 1 cut(s) 2380
BfrI CTTAAG 2 cut(s) 872, 2387
BfuAI ACCTGC 3 cut(s) 2176, 2187, 2681
BfuI GTATCC 1 cut(s) 2629
BglII AGATCT 2 cut(s) 1055, 1796
BisI GCNGC 8 cut(s) 312, 750, 1472, 1751, 1754, 2038, 2166, 2380
BlpI GCTNAGC 1 cut(s) 2563
BlsI GCNGC 8 cut(s) 313, 751, 1473, 1752, 1755, 2039, 2167, 2381
BmcAI AGTACT 1 cut(s) 2138
Bme1390I CCNGG 3 cut(s) 1722, 2128, 2310
Bme18I GGWCC 4 cut(s) 86, 508, 531, 1571
BmeT110I CYCGRG 1 cut(s) 2971
BmgT120I GGNCC 4 cut(s) 86, 508, 531, 1571
BmiI GGNNCC 3 cut(s) 469, 1573, 1881
BmrFI CCNGG 3 cut(s) 1722, 2128, 2310
BmrI ACTGGG 1 cut(s) 660
BmuI ACTGGG 1 cut(s) 660
BpiI GAAGAC 2 cut(s) 726, 1239
BpmI CTGGAG 3 cut(s) 582, 1880, 2084
Bpu10I CCTNAGC 2 cut(s) 2078, 2284
Bpu1102I GCTNAGC 1 cut(s) 2563
Bpu14I TTCGAA 2 cut(s) 1260, 1786
BpuEI CTTGAG 4 cut(s) 394, 1331, 2009, 2054
BpuMI CCSGG 1 cut(s) 2310
BsaAI YACGTR 1 cut(s) 2027
BsaBI GATNNNNATC 2 cut(s) 434, 612
BsaI GGTCTC 2 cut(s) 1261, 2671
BsaJI CCNNGG 3 cut(s) 1720, 2014, 2496
BsaXI ACNNNNNCTCC 2 cut(s) 2765, 2795
Bsc4I CCNNNNNNNGG 3 cut(s) 651, 1568, 2470
Bse118I RCCGGY 1 cut(s) 2709
Bse1I ACTGG 6 cut(s) 89, 372, 457, 655, 1975, 2101
Bse3DI GCAATG 4 cut(s) 192, 303, 747, 2674
Bse8I GATNNNNATC 2 cut(s) 434, 612
BseBI CCWGG 2 cut(s) 1722, 2128
BseDI CCNNGG 3 cut(s) 1720, 2014, 2496
BseGI GGATG 4 cut(s) 860, 871, 1192, 2430
BseJI GATNNNNATC 2 cut(s) 434, 612
BseLI CCNNNNNNNGG 3 cut(s) 651, 1568, 2470
BseMI GCAATG 4 cut(s) 192, 303, 747, 2674
BseMII CTCAG 8 cut(s) 956, 1349, 1535, 1545, 1604, 1748, 2069, 2298
BseNI ACTGG 6 cut(s) 89, 372, 457, 655, 1975, 2101
BseRI GAGGAG 5 cut(s) 558, 1313, 2095, 2435, 2749
BseXI GCAGC 7 cut(s) 298, 736, 1458, 1762, 1765, 2024, 2152
BseYI CCCAGC 1 cut(s) 763
BsgI GTGCAG 3 cut(s) 297, 624, 2709
Bsh1236I CGCG 1 cut(s) 2382
BshFI GGCC 3 cut(s) 947, 2403, 2658
BshNI GGYRCC 1 cut(s) 467
BsiHKAI GWGCWC 2 cut(s) 568, 1364
BsiHKCI CYCGRG 1 cut(s) 2971
BsiSI CCGG 2 cut(s) 2310, 2710
BslFI GGGAC 4 cut(s) 1478, 1557, 2130, 2765
BslI CCNNNNNNNGG 3 cut(s) 651, 1568, 2470
BsmAI GTCTC 5 cut(s) 1261, 1290, 2450, 2671, 2876
BsmBI CGTCTC 1 cut(s) 2450
BsmFI GGGAC 4 cut(s) 1478, 1557, 2130, 2765
BsmI GAATGC 2 cut(s) 1065, 2611
BsnI GGCC 3 cut(s) 947, 2403, 2658
Bso31I GGTCTC 2 cut(s) 1261, 2671
BsoBI CYCGRG 1 cut(s) 2971
Bsp119I TTCGAA 2 cut(s) 1260, 1786
Bsp1286I GDGCHC 2 cut(s) 568, 1364
Bsp1407I TGTACA 1 cut(s) 36
Bsp143I GATC 5 cut(s) 1055, 1796, 1879, 2525, 2728
Bsp1720I GCTNAGC 1 cut(s) 2563
Bsp19I CCATGG 2 cut(s) 2014, 2496
BspACI CCGC 2 cut(s) 2380, 2962
BspANI GGCC 3 cut(s) 947, 2403, 2658
BspCNI CTCAG 8 cut(s) 955, 1350, 1534, 1546, 1605, 1749, 2070, 2297
BspFNI CGCG 1 cut(s) 2382
BspLI GGNNCC 3 cut(s) 469, 1573, 1881
BspMAI CTGCAG 2 cut(s) 754, 1752
BspMI ACCTGC 3 cut(s) 2176, 2187, 2681
BspPI GGATC 3 cut(s) 1874, 1887, 2520
BspQI GCTCTTC 1 cut(s) 2552
BspT104I TTCGAA 2 cut(s) 1260, 1786
BspT107I GGYRCC 1 cut(s) 467
BspTI CTTAAG 2 cut(s) 872, 2387
BspTNI GGTCTC 2 cut(s) 1261, 2671
BsrDI GCAATG 4 cut(s) 192, 303, 747, 2674
BsrFI RCCGGY 1 cut(s) 2709
BsrGI TGTACA 1 cut(s) 36
BsrI ACTGG 6 cut(s) 89, 372, 457, 655, 1975, 2101
BssAI RCCGGY 1 cut(s) 2709
BssECI CCNNGG 3 cut(s) 1720, 2014, 2496
BssMI GATC 5 cut(s) 1055, 1796, 1879, 2525, 2728
BssT1I CCWWGG 2 cut(s) 2014, 2496
Bst2UI CCWGG 2 cut(s) 1722, 2128
Bst4CI ACNGT 8 cut(s) 917, 1116, 1272, 1284, 2006, 2454, 2621, 2630
Bst6I CTCTTC 5 cut(s) 593, 2412, 2552, 2715, 2910
BstAFI CTTAAG 2 cut(s) 872, 2387
BstAPI GCANNNNNTGC 1 cut(s) 746
BstAUI TGTACA 1 cut(s) 36
BstBAI YACGTR 1 cut(s) 2027
BstBI TTCGAA 2 cut(s) 1260, 1786
BstC8I GCNNGC 4 cut(s) 747, 879, 2401, 2405
BstDSI CCRYGG 2 cut(s) 2014, 2496
BstF5I GGATG 4 cut(s) 860, 871, 1192, 2430
BstFNI CGCG 1 cut(s) 2382
BstH2I RGCGCY 1 cut(s) 2380
BstHHI GCGC 1 cut(s) 2379
BstKTI GATC 5 cut(s) 1058, 1799, 1882, 2528, 2731
BstMAI GTCTC 5 cut(s) 1261, 1290, 2450, 2671, 2876
BstMBI GATC 5 cut(s) 1055, 1796, 1879, 2525, 2728
BstMWI GCNNNNNNNGC 6 cut(s) 203, 293, 572, 746, 2664, 2706
BstNI CCWGG 2 cut(s) 1722, 2128
BstNSI RCATGY 2 cut(s) 185, 990
BstSCI CCNGG 3 cut(s) 1720, 2126, 2308
BstSFI CTRYAG 3 cut(s) 750, 813, 1748
BstUI CGCG 1 cut(s) 2382
BstV1I GCAGC 7 cut(s) 298, 736, 1458, 1762, 1765, 2024, 2152
BstV2I GAAGAC 2 cut(s) 726, 1239
BstX2I RGATCY 4 cut(s) 1055, 1796, 1879, 2525
BstXI CCANNNNNNTGG 1 cut(s) 2767
BstYI RGATCY 4 cut(s) 1055, 1796, 1879, 2525
BsuI GTATCC 1 cut(s) 2629
BsuRI GGCC 3 cut(s) 947, 2403, 2658
BtgI CCRYGG 2 cut(s) 2014, 2496
BtgZI GCGATG 1 cut(s) 1857
BtsCI GGATG 4 cut(s) 860, 871, 1192, 2430
BtsI GCAGTG 1 cut(s) 2932
BtsIMutI CAGTG 7 cut(s) 36, 96, 451, 516, 682, 2607, 2932
BveI ACCTGC 3 cut(s) 2176, 2187, 2681
Cac8I GCNNGC 4 cut(s) 747, 879, 2401, 2405
CaiI CAGNNNCTG 2 cut(s) 1612, 1756
CfoI GCGC 1 cut(s) 2379
Cfr10I RCCGGY 1 cut(s) 2709
Cfr13I GGNCC 4 cut(s) 86, 508, 531, 1571
CseI GACGC 1 cut(s) 2689
Csp6I GTAC 6 cut(s) 37, 1320, 1745, 2007, 2137, 2626
CviAII CATG 9 cut(s) 182, 341, 803, 987, 1503, 1509, 2015, 2317, 2497
CviQI GTAC 6 cut(s) 37, 1320, 1745, 2007, 2137, 2626
DpnI GATC 5 cut(s) 1057, 1798, 1881, 2527, 2730
DpnII GATC 5 cut(s) 1055, 1796, 1879, 2525, 2728
DraIII CACNNNGTG 1 cut(s) 1889
DrdI GACNNNNNNGTC 1 cut(s) 920
DseDI GACNNNNNNGTC 1 cut(s) 920
Eam1104I CTCTTC 5 cut(s) 593, 2412, 2552, 2715, 2910
EarI CTCTTC 5 cut(s) 593, 2412, 2552, 2715, 2910
EciI GGCGGA 1 cut(s) 2977
Ecl136II GAGCTC 2 cut(s) 566, 1362
Eco130I CCWWGG 2 cut(s) 2014, 2496
Eco24I GRGCYC 2 cut(s) 568, 1364
Eco31I GGTCTC 2 cut(s) 1261, 2671
Eco32I GATATC 1 cut(s) 1075
Eco47I GGWCC 4 cut(s) 86, 508, 531, 1571
Eco53kI GAGCTC 2 cut(s) 566, 1362
Eco57I CTGAAG 7 cut(s) 29, 427, 540, 644, 687, 2415, 2739
Eco88I CYCGRG 1 cut(s) 2971
EcoICRI GAGCTC 2 cut(s) 566, 1362
EcoO109I RGGNCCY 1 cut(s) 531
EcoRI GAATTC 1 cut(s) 671
EcoRII CCWGG 2 cut(s) 1720, 2126
EcoRV GATATC 1 cut(s) 1075
EcoT14I CCWWGG 2 cut(s) 2014, 2496
EcoT22I ATGCAT 3 cut(s) 988, 2236, 2599
EcoT38I GRGCYC 2 cut(s) 568, 1364
ErhI CCWWGG 2 cut(s) 2014, 2496
Esp3I CGTCTC 1 cut(s) 2450
FaeI CATG 9 cut(s) 185, 344, 806, 990, 1506, 1512, 2018, 2320, 2500
FalI AAGNNNNNCTT 6 cut(s) 949, 981, 1233, 1265, 2546, 2578
FaqI GGGAC 4 cut(s) 1478, 1557, 2130, 2765
FatI CATG 9 cut(s) 181, 340, 802, 986, 1502, 1508, 2014, 2316, 2496
FauNDI CATATG 1 cut(s) 1432
FbaI TGATCA 1 cut(s) 2728
FblI GTMKAC 1 cut(s) 1599
Fnu4HI GCNGC 8 cut(s) 312, 750, 1472, 1751, 1754, 2038, 2166, 2380
FokI GGATG 4 cut(s) 867, 878, 1199, 2417
FriOI GRGCYC 2 cut(s) 568, 1364
Fsp4HI GCNGC 8 cut(s) 312, 750, 1472, 1751, 1754, 2038, 2166, 2380
FspBI CTAG 6 cut(s) 501, 695, 1536, 1664, 1775, 2150
GlaI GCGC 1 cut(s) 2378
GluI GCNGC 8 cut(s) 312, 750, 1472, 1751, 1754, 2038, 2166, 2380
GsaI CCCAGC 1 cut(s) 767
GsuI CTGGAG 3 cut(s) 582, 1880, 2084
HaeII RGCGCY 1 cut(s) 2380
HaeIII GGCC 3 cut(s) 947, 2403, 2658
HapII CCGG 2 cut(s) 2310, 2710
HgaI GACGC 1 cut(s) 2689
HhaI GCGC 1 cut(s) 2379
Hin1II CATG 9 cut(s) 185, 344, 806, 990, 1506, 1512, 2018, 2320, 2500
Hin6I GCGC 1 cut(s) 2377
HinP1I GCGC 1 cut(s) 2377
HincII GTYRAC 1 cut(s) 2715
HindII GTYRAC 1 cut(s) 2715
HindIII AAGCTT 1 cut(s) 875
HpaI GTTAAC 1 cut(s) 2715
HpaII CCGG 2 cut(s) 2310, 2710
HphI GGTGA 6 cut(s) 694, 2083, 2200, 2686, 2902, 2950
Hpy166II GTNNAC 4 cut(s) 1305, 1600, 2009, 2715
Hpy8I GTNNAC 4 cut(s) 1305, 1600, 2009, 2715
HpyCH4III ACNGT 8 cut(s) 917, 1116, 1272, 1284, 2006, 2454, 2621, 2630
HpyCH4IV ACGT 5 cut(s) 1660, 1690, 2026, 2443, 2828
HpyF10VI GCNNNNNNNGC 6 cut(s) 203, 293, 572, 746, 2664, 2706
HpySE526I ACGT 5 cut(s) 1660, 1690, 2026, 2443, 2828
Hsp92II CATG 9 cut(s) 185, 344, 806, 990, 1506, 1512, 2018, 2320, 2500
HspAI GCGC 1 cut(s) 2377
Ksp22I TGATCA 1 cut(s) 2728
KspAI GTTAAC 1 cut(s) 2715
Kzo9I GATC 5 cut(s) 1055, 1796, 1879, 2525, 2728
LguI GCTCTTC 1 cut(s) 2552
LmnI GCTCC 4 cut(s) 266, 563, 571, 2074
Lsp1109I GCAGC 7 cut(s) 298, 736, 1458, 1762, 1765, 2024, 2152
MaeI CTAG 6 cut(s) 501, 695, 1536, 1664, 1775, 2150
MaeII ACGT 5 cut(s) 1660, 1690, 2026, 2443, 2828
MaeIII GTNAC 5 cut(s) 11, 1163, 1818, 2063, 2645
MalI GATC 5 cut(s) 1057, 1798, 1881, 2527, 2730
MboI GATC 5 cut(s) 1055, 1796, 1879, 2525, 2728
MfeI CAATTG 3 cut(s) 634, 1640, 2547
MflI RGATCY 4 cut(s) 1055, 1796, 1879, 2525
MhlI GDGCHC 2 cut(s) 568, 1364
MlyI GAGTC 7 cut(s) 883, 1310, 1595, 1645, 2339, 2746, 2809
MmeI TCCRAC 2 cut(s) 2451, 2473
Mph1103I ATGCAT 3 cut(s) 988, 2236, 2599
MroXI GAANNNNTTC 4 cut(s) 219, 460, 1782, 2213
MslI CAYNNNNRTG 3 cut(s) 186, 444, 1507
MspA1I CMGCKG 1 cut(s) 1756
MspCI CTTAAG 2 cut(s) 872, 2387
MspI CCGG 2 cut(s) 2310, 2710
MspR9I CCNGG 3 cut(s) 1722, 2128, 2310
MunI CAATTG 3 cut(s) 634, 1640, 2547
Mva1269I GAATGC 2 cut(s) 1065, 2611
MvaI CCWGG 2 cut(s) 1722, 2128
MvnI CGCG 1 cut(s) 2382
MwoI GCNNNNNNNGC 6 cut(s) 203, 293, 572, 746, 2664, 2706
NciI CCSGG 1 cut(s) 2310
NcoI CCATGG 2 cut(s) 2014, 2496
NdeI CATATG 1 cut(s) 1432
NdeII GATC 5 cut(s) 1055, 1796, 1879, 2525, 2728
NlaIII CATG 9 cut(s) 185, 344, 806, 990, 1506, 1512, 2018, 2320, 2500
NlaIV GGNNCC 3 cut(s) 469, 1573, 1881
NmuCI GTSAC 2 cut(s) 1163, 1818
NsiI ATGCAT 3 cut(s) 988, 2236, 2599
NspI RCATGY 2 cut(s) 185, 990
NspV TTCGAA 2 cut(s) 1260, 1786
OliI CACNNNNGTG 1 cut(s) 444
PacI TTAATTAA 1 cut(s) 2983
PaeR7I CTCGAG 1 cut(s) 2971
PaqCI CACCTGC 1 cut(s) 2176
PciI ACATGT 1 cut(s) 181
PciSI GCTCTTC 1 cut(s) 2552
PctI GAATGC 2 cut(s) 1065, 2611
PdmI GAANNNNTTC 4 cut(s) 219, 460, 1782, 2213
PfeI GAWTC 5 cut(s) 1088, 1481, 2088, 2576, 2806
PkrI GCNGC 8 cut(s) 313, 751, 1473, 1752, 1755, 2039, 2167, 2381
PleI GAGTC 7 cut(s) 883, 1309, 1595, 1644, 2338, 2745, 2808
PpsI GAGTC 7 cut(s) 883, 1309, 1595, 1644, 2338, 2745, 2808
Ppu21I YACGTR 1 cut(s) 2027
PpuMI RGGWCCY 1 cut(s) 531
PscI ACATGT 1 cut(s) 181
PshBI ATTAAT 2 cut(s) 1125, 2979
Psp124BI GAGCTC 2 cut(s) 568, 1364
Psp1406I AACGTT 1 cut(s) 2828
Psp5II RGGWCCY 1 cut(s) 531
Psp6I CCWGG 2 cut(s) 1720, 2126
PspFI CCCAGC 1 cut(s) 763
PspGI CCWGG 2 cut(s) 1720, 2126
PspN4I GGNNCC 3 cut(s) 469, 1573, 1881
PspPI GGNCC 4 cut(s) 86, 508, 531, 1571
PspPPI RGGWCCY 1 cut(s) 531
PspXI VCTCGAGB 1 cut(s) 2971
PstI CTGCAG 2 cut(s) 754, 1752
PstNI CAGNNNCTG 2 cut(s) 1612, 1756
PsuI RGATCY 4 cut(s) 1055, 1796, 1879, 2525
PvuII CAGCTG 1 cut(s) 1756
RsaI GTAC 6 cut(s) 38, 1321, 1746, 2008, 2138, 2627
RsaNI GTAC 6 cut(s) 37, 1320, 1745, 2007, 2137, 2626
RseI CAYNNNNRTG 3 cut(s) 186, 444, 1507
SacI GAGCTC 2 cut(s) 568, 1364
SapI GCTCTTC 1 cut(s) 2552
SatI GCNGC 8 cut(s) 312, 750, 1472, 1751, 1754, 2038, 2166, 2380
Sau3AI GATC 5 cut(s) 1055, 1796, 1879, 2525, 2728
Sau96I GGNCC 4 cut(s) 86, 508, 531, 1571
ScaI AGTACT 1 cut(s) 2138
SchI GAGTC 7 cut(s) 883, 1310, 1595, 1645, 2339, 2746, 2809
ScrFI CCNGG 3 cut(s) 1722, 2128, 2310
SduI GDGCHC 2 cut(s) 568, 1364
SfcI CTRYAG 3 cut(s) 750, 813, 1748
Sfr274I CTCGAG 1 cut(s) 2971
SfuI TTCGAA 2 cut(s) 1260, 1786
SinI GGWCC 4 cut(s) 86, 508, 531, 1571
SlaI CTCGAG 1 cut(s) 2971
SmiMI CAYNNNNRTG 3 cut(s) 186, 444, 1507
SmlI CTYRAG 7 cut(s) 409, 872, 1346, 1988, 2069, 2387, 2971
SmoI CTYRAG 7 cut(s) 409, 872, 1346, 1988, 2069, 2387, 2971
SsiI CCGC 2 cut(s) 2380, 2962
SspI AATATT 2 cut(s) 475, 2145
SspMI CTAG 6 cut(s) 501, 695, 1536, 1664, 1775, 2150
SstI GAGCTC 2 cut(s) 568, 1364
StyD4I CCNGG 3 cut(s) 1720, 2126, 2308
StyI CCWWGG 2 cut(s) 2014, 2496
TaaI ACNGT 8 cut(s) 917, 1116, 1272, 1284, 2006, 2454, 2621, 2630
TaiI ACGT 5 cut(s) 1663, 1693, 2029, 2446, 2831
TaqI TCGA 8 cut(s) 223, 887, 892, 951, 1260, 1549, 1786, 2972
TaqII GACCGA 1 cut(s) 2829
TatI WGTACW 4 cut(s) 36, 1744, 2136, 2625
TauI GCSGC 1 cut(s) 2382
TfiI GAWTC 5 cut(s) 1088, 1481, 2088, 2576, 2806
TscAI CASTG 7 cut(s) 36, 96, 451, 523, 682, 2607, 2932
TseFI GTSAC 2 cut(s) 1163, 1818
TseI GCWGC 7 cut(s) 311, 749, 1471, 1750, 1753, 2037, 2165
Tsp45I GTSAC 2 cut(s) 1163, 1818
TspGWI ACGGA 1 cut(s) 1218
TspRI CASTG 7 cut(s) 36, 96, 451, 523, 682, 2607, 2932
Vha464I CTTAAG 2 cut(s) 872, 2387
VpaK11BI GGWCC 4 cut(s) 86, 508, 531, 1571
VspI ATTAAT 2 cut(s) 1125, 2979
XapI RAATTY 7 cut(s) 345, 459, 671, 1226, 1628, 2213, 2587
XbaI TCTAGA 4 cut(s) 500, 694, 1663, 1774
XceI RCATGY 2 cut(s) 185, 990
XcmI CCANNNNNNNNNTGG 4 cut(s) 214, 1247, 2666, 2767
XhoI CTCGAG 1 cut(s) 2971
XmiI GTMKAC 1 cut(s) 1599
XmnI GAANNNNTTC 4 cut(s) 219, 460, 1782, 2213
XspI CTAG 6 cut(s) 501, 695, 1536, 1664, 1775, 2150
ZrmI AGTACT 1 cut(s) 2138
Zsp2I ATGCAT 3 cut(s) 988, 2236, 2599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.