pycom16g00530

Transcription initiation factor TFIID subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0007884
Physical Location & Seq
Forward (+)
326371 .. 329567
3197 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1170 bp
ATGGCAACCTATACAGGAAAAGGAGCCCCTTCAAATGGGTCTGTTTATGTTTGCAACTTGCCTGAGGGGACTGATGAAAATATGTTGGCTGAACATTTTGGTACCATTGGCTTGTTAAAGAAAGACAAGCGAACAGGTCGACCAAAAATTTGGCTATACCGAGACAAATTAACCAACGAACCCAAGGGAGATGCTACTGTAACATATGAAGATCCACATGCTGCATCAGCGGCCGTTGAATGGTTTAATGACAAGGAATTCCATGGTAATACCATTGGGGTTTTTATAGCTGAGTCCAAAAGCAAAGACGATCAAACTCATAACACTGTGGTTGATCCAAGTTTTGGCAATGATTTTGATGGACCGGAGGAAGCTGCTCAGGATACGAATGGGGGTGGCAGCGGCGGTAGAGGTCGAGGTGATGCTACTGGGAAAGCATGGCAACAAGAAGGAGACTGGACGTGCCCGAATACAAGCAGCTGTGCCAATGTGAATTTTGCATTTCGTGGTGTGTGCAATCGCTGTGGAAGTGCTCGACCTACCGGTGCTTCTGGTGGCGCAGGGGGTGGTGGTCGTGGCAGGGGTCGTGGCGTTGATTCTGGGGGCCGTGGTGGTCCTGTTGGTGCTCCTACAGGTGGACTCTTTGGTCCAAATGACTGGCCTTGTCCAATGTGTGCCAACATCAATTGGGCAAAGCGTACAAAATGCAATATATGCAACACAAATAAACCTGGTCACAATGAAGGGGGTGTAAGGGGGGGACGTGGTGGTGGATACAAGGAACTTGATGAAGAAGAATTAGAGGAAATCAAGCGACGTAGACGGGAGGCAGAACAAGATGATGGAGAGATGTATGATGAGTTTGGCAACCTCAAGAAAAAATTTCGCGCTAAAACACAGCCAACTGAAACTGGACGAGCTCTTCCAGGTGCTGGACGTGCTGGATGGGAGGTGGAGGAAATAGGTGCTATTGACAGAGATGGCAGAGAGAGAAATAGAGAGAGGGGAAGGGAACGCGATGATAGACCGAGCAGCAAGAATAGAGACAGAGATGATGGTTATAGGCGTCGCAGTCGAAGTAGAGAAAGGGATAGGGATAGGGATAGGGGGAGAGATAGACCTCGAGACTATGATTATGATCGAGAAAAAGAATACGGGAGGGACTACTAG

Protein Analysis

390

Amino Acids

42.85

Weight (kDa)

7.53

Isoelectric Point (pI)

35.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 15 - 92 1e-08 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 645
Acc65I GGTACC 1 cut(s) 101
AccB1I GGYRCC 1 cut(s) 101
AccB7I CCANNNNNTGG 2 cut(s) 344, 932
AccI GTMKAC 2 cut(s) 139, 820
AccII CGCG 2 cut(s) 888, 1017
AciI CCGC 3 cut(s) 230, 402, 405
AclWI GGATC 2 cut(s) 206, 329
AcoI YGGCCR 1 cut(s) 231
AcsI RAATTY 4 cut(s) 147, 257, 493, 881
AcyI GRCGYC 1 cut(s) 1066
AfaI GTAC 2 cut(s) 103, 700
AfiI CCNNNNNNNGG 5 cut(s) 35, 240, 344, 635, 932
AgeI ACCGGT 1 cut(s) 542
AgsI TTSAA 2 cut(s) 33, 239
AjiI CACGTC 3 cut(s) 462, 764, 938
AjnI CCWGG 2 cut(s) 730, 925
AluBI AGCT 4 cut(s) 290, 374, 480, 920
AluI AGCT 4 cut(s) 290, 374, 480, 920
Alw21I GWGCWC 3 cut(s) 535, 628, 922
Alw26I GTCTC 4 cut(s) 156, 447, 1038, 1119
AlwI GGATC 2 cut(s) 206, 329
AlwNI CAGNNNCTG 1 cut(s) 932
Ama87I CYCGRG 1 cut(s) 1122
AoxI GGCC 3 cut(s) 231, 604, 659
ApeKI GCWGC 5 cut(s) 221, 374, 399, 477, 1032
ApoI RAATTY 4 cut(s) 147, 257, 493, 881
ArsI GACNNNNNNTTYG 2 cut(s) 132, 164
AsiGI ACCGGT 1 cut(s) 542
Asp718I GGTACC 1 cut(s) 101
AspLEI GCGC 2 cut(s) 560, 890
AspS9I GGNCC 4 cut(s) 362, 604, 614, 647
AsuHPI GGTGA 1 cut(s) 431
AvaI CYCGRG 1 cut(s) 1122
AvaII GGWCC 3 cut(s) 362, 614, 647
AxyI CCTNAGG 1 cut(s) 63
BaeGI GKGCMC 1 cut(s) 467
BanI GGYRCC 1 cut(s) 101
BanII GRGCYC 2 cut(s) 28, 922
Bbv12I GWGCWC 3 cut(s) 535, 628, 922
BbvI GCAGC 5 cut(s) 208, 361, 411, 489, 1044
BccI CCATC 5 cut(s) 353, 836, 939, 974, 1049
BceAI ACGGC 2 cut(s) 218, 591
BciT130I CCWGG 2 cut(s) 732, 927
BciVI GTATCC 2 cut(s) 376, 767
BcoDI GTCTC 4 cut(s) 156, 447, 1038, 1119
BfaI CTAG 1 cut(s) 1168
BfmI CTRYAG 1 cut(s) 630
BfuI GTATCC 2 cut(s) 376, 767
BisI GCNGC 7 cut(s) 222, 231, 375, 400, 403, 478, 1033
BlsI GCNGC 7 cut(s) 223, 232, 376, 401, 404, 479, 1034
Bme1390I CCNGG 2 cut(s) 732, 927
Bme18I GGWCC 3 cut(s) 362, 614, 647
BmeT110I CYCGRG 1 cut(s) 1122
BmgBI CACGTC 3 cut(s) 462, 764, 938
BmgT120I GGNCC 4 cut(s) 362, 604, 614, 647
BmiI GGNNCC 3 cut(s) 25, 103, 605
BmrFI CCNGG 2 cut(s) 732, 927
BmrI ACTGGG 1 cut(s) 438
BmsI GCATC 3 cut(s) 181, 233, 412
BmuI ACTGGG 1 cut(s) 438
Bpu10I CCTNAGC 1 cut(s) 378
BpuEI CTTGAG 1 cut(s) 857
BsaBI GATNNNNATC 1 cut(s) 1137
BsaHI GRCGYC 1 cut(s) 1066
BsaJI CCNNGG 3 cut(s) 183, 262, 607
BsaWI WCCGGW 2 cut(s) 364, 542
Bsc4I CCNNNNNNNGG 5 cut(s) 35, 240, 344, 635, 932
Bse118I RCCGGY 1 cut(s) 542
Bse1I ACTGG 4 cut(s) 433, 461, 662, 916
Bse21I CCTNAGG 1 cut(s) 63
Bse3DI GCAATG 1 cut(s) 355
Bse8I GATNNNNATC 1 cut(s) 1137
BseBI CCWGG 2 cut(s) 732, 927
BseDI CCNNGG 3 cut(s) 183, 262, 607
BseGI GGATG 1 cut(s) 950
BseJI GATNNNNATC 1 cut(s) 1137
BseLI CCNNNNNNNGG 5 cut(s) 35, 240, 344, 635, 932
BseMI GCAATG 1 cut(s) 355
BseMII CTCAG 3 cut(s) 54, 282, 392
BseNI ACTGG 4 cut(s) 433, 461, 662, 916
BseSI GKGCMC 1 cut(s) 467
BseX3I CGGCCG 1 cut(s) 231
BseXI GCAGC 5 cut(s) 208, 361, 411, 489, 1044
Bsh1236I CGCG 2 cut(s) 888, 1017
Bsh1285I CGRYCG 1 cut(s) 234
BshFI GGCC 3 cut(s) 233, 606, 661
BshNI GGYRCC 1 cut(s) 101
BshTI ACCGGT 1 cut(s) 542
BsiEI CGRYCG 1 cut(s) 234
BsiHKAI GWGCWC 3 cut(s) 535, 628, 922
BsiHKCI CYCGRG 1 cut(s) 1122
BsiSI CCGG 2 cut(s) 365, 543
BslFI GGGAC 2 cut(s) 82, 774
BslI CCNNNNNNNGG 5 cut(s) 35, 240, 344, 635, 932
BsmAI GTCTC 4 cut(s) 156, 447, 1038, 1119
BsmFI GGGAC 2 cut(s) 82, 774
BsnI GGCC 3 cut(s) 233, 606, 661
BsoBI CYCGRG 1 cut(s) 1122
Bsp1286I GDGCHC 5 cut(s) 28, 467, 535, 628, 922
Bsp143I GATC 4 cut(s) 211, 310, 334, 1138
Bsp19I CCATGG 1 cut(s) 262
BspACI CCGC 3 cut(s) 230, 402, 405
BspANI GGCC 3 cut(s) 233, 606, 661
BspCNI CTCAG 3 cut(s) 55, 283, 391
BspFNI CGCG 2 cut(s) 888, 1017
BspLI GGNNCC 3 cut(s) 25, 103, 605
BspPI GGATC 2 cut(s) 206, 329
BspQI GCTCTTC 1 cut(s) 927
BspT107I GGYRCC 1 cut(s) 101
BsrDI GCAATG 1 cut(s) 355
BsrFI RCCGGY 1 cut(s) 542
BsrI ACTGG 4 cut(s) 433, 461, 662, 916
BssAI RCCGGY 1 cut(s) 542
BssECI CCNNGG 3 cut(s) 183, 262, 607
BssMI GATC 4 cut(s) 211, 310, 334, 1138
BssNI GRCGYC 1 cut(s) 1066
BssT1I CCWWGG 2 cut(s) 183, 262
Bst2UI CCWGG 2 cut(s) 732, 927
Bst4CI ACNGT 2 cut(s) 199, 328
Bst6I CTCTTC 1 cut(s) 927
BstACI GRCGYC 1 cut(s) 1066
BstAPI GCANNNNNTGC 1 cut(s) 714
BstDEI CTNAG 3 cut(s) 63, 291, 378
BstDSI CCRYGG 2 cut(s) 262, 607
BstF5I GGATG 1 cut(s) 950
BstFNI CGCG 2 cut(s) 888, 1017
BstHHI GCGC 2 cut(s) 560, 890
BstKTI GATC 4 cut(s) 214, 313, 337, 1141
BstMAI GTCTC 4 cut(s) 156, 447, 1038, 1119
BstMBI GATC 4 cut(s) 211, 310, 334, 1138
BstMCI CGRYCG 1 cut(s) 234
BstMWI GCNNNNNNNGC 4 cut(s) 227, 230, 714, 938
BstNI CCWGG 2 cut(s) 732, 927
BstNSI RCATGY 1 cut(s) 221
BstSCI CCNGG 2 cut(s) 730, 925
BstSFI CTRYAG 1 cut(s) 630
BstSLI GKGCMC 1 cut(s) 467
BstUI CGCG 2 cut(s) 888, 1017
BstV1I GCAGC 5 cut(s) 208, 361, 411, 489, 1044
BstX2I RGATCY 1 cut(s) 211
BstXI CCANNNNNNTGG 2 cut(s) 150, 657
BstYI RGATCY 1 cut(s) 211
BstZI CGGCCG 1 cut(s) 231
Bsu36I CCTNAGG 1 cut(s) 63
BsuI GTATCC 2 cut(s) 376, 767
BsuRI GGCC 3 cut(s) 233, 606, 661
BtgI CCRYGG 2 cut(s) 262, 607
BtgZI GCGATG 1 cut(s) 1032
BtrI CACGTC 3 cut(s) 462, 764, 938
BtsCI GGATG 1 cut(s) 950
BtsIMutI CAGTG 1 cut(s) 324
CaiI CAGNNNCTG 1 cut(s) 932
CfoI GCGC 2 cut(s) 560, 890
Cfr10I RCCGGY 1 cut(s) 542
Cfr13I GGNCC 4 cut(s) 362, 604, 614, 647
CseI GACGC 1 cut(s) 1055
CsiI ACCWGGT 1 cut(s) 730
Csp6I GTAC 2 cut(s) 102, 699
CspAI ACCGGT 1 cut(s) 542
CspCI CAANNNNNGTGG 2 cut(s) 505, 540
CviAII CATG 3 cut(s) 218, 263, 438
CviQI GTAC 2 cut(s) 102, 699
DdeI CTNAG 3 cut(s) 63, 291, 378
DpnI GATC 4 cut(s) 213, 312, 336, 1140
DpnII GATC 4 cut(s) 211, 310, 334, 1138
DrdI GACNNNNNNGTC 1 cut(s) 645
DseDI GACNNNNNNGTC 1 cut(s) 645
EaeI YGGCCR 1 cut(s) 231
EagI CGGCCG 1 cut(s) 231
Eam1104I CTCTTC 1 cut(s) 927
EarI CTCTTC 1 cut(s) 927
Ecl136II GAGCTC 1 cut(s) 920
EclXI CGGCCG 1 cut(s) 231
Eco130I CCWWGG 2 cut(s) 183, 262
Eco24I GRGCYC 2 cut(s) 28, 922
Eco47I GGWCC 3 cut(s) 362, 614, 647
Eco52I CGGCCG 1 cut(s) 231
Eco53kI GAGCTC 1 cut(s) 920
Eco81I CCTNAGG 1 cut(s) 63
Eco88I CYCGRG 1 cut(s) 1122
EcoICRI GAGCTC 1 cut(s) 920
EcoRI GAATTC 1 cut(s) 257
EcoRII CCWGG 2 cut(s) 730, 925
EcoT14I CCWWGG 2 cut(s) 183, 262
EcoT38I GRGCYC 2 cut(s) 28, 922
ErhI CCWWGG 2 cut(s) 183, 262
FaeI CATG 3 cut(s) 221, 266, 441
FaqI GGGAC 2 cut(s) 82, 774
FatI CATG 3 cut(s) 217, 262, 437
FauNDI CATATG 1 cut(s) 205
FblI GTMKAC 2 cut(s) 139, 820
Fnu4HI GCNGC 7 cut(s) 222, 231, 375, 400, 403, 478, 1033
FokI GGATG 1 cut(s) 957
FriOI GRGCYC 2 cut(s) 28, 922
Fsp4HI GCNGC 7 cut(s) 222, 231, 375, 400, 403, 478, 1033
FspBI CTAG 1 cut(s) 1168
GlaI GCGC 2 cut(s) 559, 889
GluI GCNGC 7 cut(s) 222, 231, 375, 400, 403, 478, 1033
HaeIII GGCC 3 cut(s) 233, 606, 661
HapII CCGG 2 cut(s) 365, 543
HgaI GACGC 1 cut(s) 1055
HhaI GCGC 2 cut(s) 560, 890
Hin1I GRCGYC 1 cut(s) 1066
Hin1II CATG 3 cut(s) 221, 266, 441
Hin6I GCGC 2 cut(s) 558, 888
HinP1I GCGC 2 cut(s) 558, 888
HincII GTYRAC 1 cut(s) 140
HindII GTYRAC 1 cut(s) 140
HinfI GANTC 3 cut(s) 293, 596, 639
HpaII CCGG 2 cut(s) 365, 543
HphI GGTGA 1 cut(s) 431
Hpy166II GTNNAC 3 cut(s) 140, 638, 821
Hpy188III TCNNGA 4 cut(s) 380, 874, 1124, 1142
Hpy8I GTNNAC 3 cut(s) 140, 638, 821
Hpy99I CGWCG 2 cut(s) 819, 1071
HpyAV CCTTC 4 cut(s) 39, 443, 737, 1002
HpyCH4III ACNGT 2 cut(s) 199, 328
HpyCH4IV ACGT 4 cut(s) 461, 763, 817, 937
HpyCH4V TGCA 6 cut(s) 54, 224, 500, 516, 708, 717
HpyF10VI GCNNNNNNNGC 4 cut(s) 227, 230, 714, 938
HpyF3I CTNAG 3 cut(s) 63, 291, 378
HpySE526I ACGT 4 cut(s) 461, 763, 817, 937
Hsp92I GRCGYC 1 cut(s) 1066
Hsp92II CATG 3 cut(s) 221, 266, 441
HspAI GCGC 2 cut(s) 558, 888
KpnI GGTACC 1 cut(s) 105
Kzo9I GATC 4 cut(s) 211, 310, 334, 1138
LguI GCTCTTC 1 cut(s) 927
LmnI GCTCC 2 cut(s) 23, 631
Lsp1109I GCAGC 5 cut(s) 208, 361, 411, 489, 1044
LweI GCATC 3 cut(s) 181, 233, 412
MabI ACCWGGT 1 cut(s) 730
MaeI CTAG 1 cut(s) 1168
MaeII ACGT 4 cut(s) 461, 763, 817, 937
MaeIII GTNAC 2 cut(s) 199, 734
MalI GATC 4 cut(s) 213, 312, 336, 1140
MboI GATC 4 cut(s) 211, 310, 334, 1138
MboII GAAGA 4 cut(s) 221, 803, 806, 914
MfeI CAATTG 1 cut(s) 685
MflI RGATCY 1 cut(s) 211
MhlI GDGCHC 5 cut(s) 28, 467, 535, 628, 922
MluCI AATT 7 cut(s) 147, 167, 257, 493, 685, 797, 881
MlyI GAGTC 2 cut(s) 302, 633
MseI TTAA 3 cut(s) 116, 170, 246
MspA1I CMGCKG 3 cut(s) 230, 402, 480
MspI CCGG 2 cut(s) 365, 543
MspR9I CCNGG 2 cut(s) 732, 927
MunI CAATTG 1 cut(s) 685
MvaI CCWGG 2 cut(s) 732, 927
MvnI CGCG 2 cut(s) 888, 1017
MwoI GCNNNNNNNGC 4 cut(s) 227, 230, 714, 938
NcoI CCATGG 1 cut(s) 262
NdeI CATATG 1 cut(s) 205
NdeII GATC 4 cut(s) 211, 310, 334, 1138
NlaIII CATG 3 cut(s) 221, 266, 441
NlaIV GGNNCC 3 cut(s) 25, 103, 605
NmuCI GTSAC 1 cut(s) 734
NspI RCATGY 1 cut(s) 221
PaeR7I CTCGAG 1 cut(s) 1122
PciSI GCTCTTC 1 cut(s) 927
PfeI GAWTC 1 cut(s) 596
PflMI CCANNNNNTGG 2 cut(s) 344, 932
PinAI ACCGGT 1 cut(s) 542
PkrI GCNGC 7 cut(s) 223, 232, 376, 401, 404, 479, 1034
PleI GAGTC 2 cut(s) 301, 633
PpsI GAGTC 2 cut(s) 301, 633
Psp124BI GAGCTC 1 cut(s) 922
Psp6I CCWGG 2 cut(s) 730, 925
PspGI CCWGG 2 cut(s) 730, 925
PspN4I GGNNCC 3 cut(s) 25, 103, 605
PspPI GGNCC 4 cut(s) 362, 604, 614, 647
PstNI CAGNNNCTG 1 cut(s) 932
PsuI RGATCY 1 cut(s) 211
PvuII CAGCTG 1 cut(s) 480
RsaI GTAC 2 cut(s) 103, 700
RsaNI GTAC 2 cut(s) 102, 699
SacI GAGCTC 1 cut(s) 922
SalI GTCGAC 1 cut(s) 138
SapI GCTCTTC 1 cut(s) 927
SaqAI TTAA 3 cut(s) 116, 170, 246
SatI GCNGC 7 cut(s) 222, 231, 375, 400, 403, 478, 1033
Sau3AI GATC 4 cut(s) 211, 310, 334, 1138
Sau96I GGNCC 4 cut(s) 362, 604, 614, 647
SchI GAGTC 2 cut(s) 302, 633
ScrFI CCNGG 2 cut(s) 732, 927
SduI GDGCHC 5 cut(s) 28, 467, 535, 628, 922
SexAI ACCWGGT 1 cut(s) 730
SfaNI GCATC 3 cut(s) 181, 233, 412
SfcI CTRYAG 1 cut(s) 630
Sfr274I CTCGAG 1 cut(s) 1122
SinI GGWCC 3 cut(s) 362, 614, 647
SlaI CTCGAG 1 cut(s) 1122
SmlI CTYRAG 2 cut(s) 872, 1122
SmoI CTYRAG 2 cut(s) 872, 1122
Sse9I AATT 7 cut(s) 147, 167, 257, 493, 685, 797, 881
SsiI CCGC 3 cut(s) 230, 402, 405
SspMI CTAG 1 cut(s) 1168
SstI GAGCTC 1 cut(s) 922
StyD4I CCNGG 2 cut(s) 730, 925
StyI CCWWGG 2 cut(s) 183, 262
TaaI ACNGT 2 cut(s) 199, 328
TaiI ACGT 4 cut(s) 464, 766, 820, 940
TaqI TCGA 6 cut(s) 139, 415, 535, 1075, 1123, 1141
TaqII GACCGA 1 cut(s) 1042
TasI AATT 7 cut(s) 147, 167, 257, 493, 685, 797, 881
TauI GCSGC 2 cut(s) 233, 405
TfiI GAWTC 1 cut(s) 596
Tru1I TTAA 3 cut(s) 116, 170, 246
Tru9I TTAA 3 cut(s) 116, 170, 246
TscAI CASTG 1 cut(s) 331
TseFI GTSAC 1 cut(s) 734
TseI GCWGC 5 cut(s) 221, 374, 399, 477, 1032
Tsp45I GTSAC 1 cut(s) 734
TspDTI ATGAA 4 cut(s) 90, 222, 756, 804
TspRI CASTG 1 cut(s) 331
Van91I CCANNNNNTGG 2 cut(s) 344, 932
VpaK11BI GGWCC 3 cut(s) 362, 614, 647
XapI RAATTY 4 cut(s) 147, 257, 493, 881
XceI RCATGY 1 cut(s) 221
XhoI CTCGAG 1 cut(s) 1122
XmiI GTMKAC 2 cut(s) 139, 820
XspI CTAG 1 cut(s) 1168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.