pycom16g00570

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0008096
Physical Location & Seq
Reverse (-)
348948 .. 352645
3698 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1692 bp
ATGGTTGTGATCATGATGCAGCAGCAGACTTCTGCAGCACCCCCGTCGCACTCAATCACTACTCCCCCTTCCTCCACCACCACCCCCACTGCCATTAGATCACCCACGGCAACCCCTACACACTGCCAGCAGCACCCCTCGCCTCTCCACCGCCATGCTCTTCCTGTCCCACCGCCACCTCTCGACCACGTGCTGCCGATTGCGGCCGCCCACCCGTCCGGCGAGCCTGCGCCGACAATTCAGTGCCAGCTCGCGAGGGTCCGGCTCTCAGATATTGCCCCGTACCACGGGGCACCCGGTGGGTCGTATGTGAGGGCGGTGGAGGCGCTCTCGGGGTCTTTAACGAGGCACAATGCGGCTGTCATCGAATTGGGGAGCGAAGACGCGGCGCTCCTGCGGTGCGGCTTGGAAGCCTCGAGGTTGTATTTTAGGAGTAGAGCTCAGCTCGGTGTTGGGAAAGGAAGCCGTGGAGTTCACATGTACAGAGCTGGCAGGGCTTTGGAAGATTGGGACTCATCCCCACCGTGCATGGCGGATATTTTTAGGTGCATGGGCAAGGCGGCCCATGTGGCTTTGTGTGCAGTAGCTAGGCATCTTCGTTTGCGAAGCGATGTTTTCAACCATTTGCTTGACGATACTCCATTGCCTGCTAATGAGGTGTCGTCGTCAGTTCTGGTTGCAACATACTCAAATGCTTCCCTGCAAAATGGAAAAGGTGCTATTGGGGGAGGGAAGCCAGGAATGAATGCTGAAGTTGAGAAGGGATTATTAACATTGGTTTCCTCAGATGGTCCTGGTATACAGGTATGTGACCCCAACGGTCGTTGGTACCTAGCAGATGGTGGGTCATCTCCTGGGGATCTTTTACTACTTACAGGTAAGGCACTCAGCCATGCTACTGCTGGCCTCCGCCCAACTGCACCGTATAAAGCTGCCCCCGATTACTCTTCAGGCATTAGTAGTGGTGGGAGGACATCGCTTGCATTTAGGCTCATGCCACAAGGTAACGCTATATTAGATTGTTCTCCAGTTGCAGCAGCTGGTCATGTTATTCCCCACAGCTATGTCCCAATATCTGTAAGCCAGTTTATGGATGATCTTTCTGCTGAGGAAGATGTATTGGGCAGTCGTTCTGACAACGCTTATGTAGCTCGAAACAATTTAAACAAGGAACCATCTTTAAGAAGCGTTCTCTCAGATCCCTTATCTGGGTCATTCCTCGAAGATGCGATGTTAGTCTCTTGTGGGCATTCATTTGGTGGTCTCACGTTGAGAAGAGTCATTGAAACAACAAGATGCACCCTCTGTGGTGCAGATATTGAGACGGGGTCTTTGGTTCCTAATCATGCGCTTAGAGCTGCAGCTGCAGCAGTCAAGCATGAGGATGATCGAAGGCTATTTCATAATGCGACTGTCCGAAAGCGTCGGAAAGAATTAGTTGACCAAATGGATTCATCAAGAAGACCAAACAAGGAAAATGGAGATGTTGGTGCAGATGGGATTCGTGGGGTGCAGTACCCATTTTCTGTGAATGAAAAAGTGGTAATTAAGGGGAACAGGAGGACTCCAGAAAAATTTGTTGGCAAGGAAGCGGTCATCACATCCCAGTGTCTCAATGGCTGGTACTTGCTCAAGATTGTTGGAACTGGAGAGAATGTTCGGTTGCAATACCGTTCACTGAGGAAGATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

564

Amino Acids

60.0

Weight (kDa)

8.93

Isoelectric Point (pI)

52.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUB62-63_C PF23112 504 - 562 2.3e-36 U-box protein 62/63 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 415
Acc65I GGTACC 1 cut(s) 828
AccB1I GGYRCC 2 cut(s) 292, 828
AccB7I CCANNNNNTGG 1 cut(s) 1090
AccI GTMKAC 1 cut(s) 799
AccII CGCG 2 cut(s) 254, 386
AclWI GGATC 2 cut(s) 867, 1193
AcoI YGGCCR 1 cut(s) 204
AcsI RAATTY 1 cut(s) 1574
AcuI CTGAAG 2 cut(s) 771, 933
AcvI CACGTG 1 cut(s) 190
AdeI CACNNNGTG 1 cut(s) 299
AfaI GTAC 5 cut(s) 284, 482, 830, 1517, 1625
AfiI CCNNNNNNNGG 4 cut(s) 287, 1090, 1208, 1209
AflIII ACRYGT 1 cut(s) 477
AgsI TTSAA 2 cut(s) 619, 1286
AjnI CCWGG 3 cut(s) 736, 793, 853
AjuI GAANNNNNNNTTGG 2 cut(s) 1563, 1595
AloI GAACNNNNNNTCC 2 cut(s) 1641, 1673
Alw21I GWGCWC 1 cut(s) 442
Alw26I GTCTC 4 cut(s) 1243, 1268, 1316, 1616
AlwI GGATC 2 cut(s) 867, 1193
AlwNI CAGNNNCTG 1 cut(s) 1040
Ama87I CYCGRG 2 cut(s) 331, 415
AoxI GGCC 3 cut(s) 204, 561, 904
ApoI RAATTY 1 cut(s) 1574
ArsI GACNNNNNNTTYG 2 cut(s) 1315, 1347
Asp718I GGTACC 1 cut(s) 828
AspLEI GCGC 4 cut(s) 232, 328, 391, 1351
AspS9I GGNCC 3 cut(s) 259, 562, 791
AsuC2I CCSGG 1 cut(s) 297
AsuHPI GGTGA 1 cut(s) 93
AvaI CYCGRG 2 cut(s) 331, 415
AvaII GGWCC 2 cut(s) 259, 791
BaeGI GKGCMC 1 cut(s) 295
BanI GGYRCC 2 cut(s) 292, 828
BanII GRGCYC 1 cut(s) 442
BbrPI CACGTG 1 cut(s) 190
BbsI GAAGAC 2 cut(s) 387, 1468
Bbv12I GWGCWC 1 cut(s) 442
BbvCI CCTCAGC 1 cut(s) 1107
BccI CCATC 4 cut(s) 782, 833, 1183, 1490
BceAI ACGGC 2 cut(s) 123, 450
BcgI CGANNNNNNTGC 2 cut(s) 27, 61
BciT130I CCWGG 3 cut(s) 738, 795, 855
BclI TGATCA 1 cut(s) 9
BcnI CCSGG 1 cut(s) 297
BcoDI GTCTC 4 cut(s) 1243, 1268, 1316, 1616
BfaI CTAG 2 cut(s) 588, 833
BfmI CTRYAG 3 cut(s) 33, 1359, 1365
BfoI RGCGCY 2 cut(s) 329, 392
BglI GCCNNNNNGGC 1 cut(s) 569
BlpI GCTNAGC 1 cut(s) 441
Bme1390I CCNGG 4 cut(s) 297, 738, 795, 855
Bme18I GGWCC 2 cut(s) 259, 791
BmeT110I CYCGRG 2 cut(s) 331, 415
BmgT120I GGNCC 3 cut(s) 259, 562, 791
BmiI GGNNCC 5 cut(s) 260, 294, 830, 1173, 1338
BmrFI CCNGG 4 cut(s) 297, 738, 795, 855
BmrI ACTGGG 1 cut(s) 1600
BmsI GCATC 4 cut(s) 6, 601, 1216, 1286
BmuI ACTGGG 1 cut(s) 1600
BpiI GAAGAC 2 cut(s) 387, 1468
BplI GAGNNNNNCTC 6 cut(s) 314, 346, 424, 456, 429, 461
BpmI CTGGAG 3 cut(s) 1011, 1551, 1668
Bpu10I CCTNAGC 1 cut(s) 1107
Bpu1102I GCTNAGC 1 cut(s) 441
BpuEI CTTGAG 1 cut(s) 1616
BpuMI CCSGG 1 cut(s) 297
BsaAI YACGTR 1 cut(s) 190
BsaI GGTCTC 1 cut(s) 1268
BsaJI CCNNGG 4 cut(s) 105, 286, 466, 854
BsaXI ACNNNNNCTCC 2 cut(s) 1641, 1671
Bsc4I CCNNNNNNNGG 4 cut(s) 287, 1090, 1208, 1209
Bse1I ACTGG 4 cut(s) 1028, 1084, 1606, 1651
Bse3DI GCAATG 1 cut(s) 641
BseBI CCWGG 3 cut(s) 738, 795, 855
BseDI CCNNGG 4 cut(s) 105, 286, 466, 854
BseGI GGATG 4 cut(s) 515, 1099, 1390, 1601
BseLI CCNNNNNNNGG 4 cut(s) 287, 1090, 1208, 1209
BseMI GCAATG 1 cut(s) 641
BseMII CTCAG 7 cut(s) 282, 455, 798, 901, 1098, 1209, 1670
BseNI ACTGG 4 cut(s) 1028, 1084, 1606, 1651
BseSI GKGCMC 1 cut(s) 295
BseX3I CGGCCG 1 cut(s) 204
BsgI GTGCAG 5 cut(s) 600, 903, 1332, 1512, 1532
Bsh1236I CGCG 2 cut(s) 254, 386
Bsh1285I CGRYCG 2 cut(s) 207, 823
BshFI GGCC 3 cut(s) 206, 563, 906
BshNI GGYRCC 2 cut(s) 292, 828
BsiEI CGRYCG 2 cut(s) 207, 823
BsiHKAI GWGCWC 1 cut(s) 442
BsiHKCI CYCGRG 2 cut(s) 331, 415
BsiSI CCGG 3 cut(s) 219, 262, 297
BslFI GGGAC 3 cut(s) 152, 524, 1052
BslI CCNNNNNNNGG 4 cut(s) 287, 1090, 1208, 1209
BsmAI GTCTC 4 cut(s) 1243, 1268, 1316, 1616
BsmBI CGTCTC 1 cut(s) 1316
BsmFI GGGAC 3 cut(s) 152, 524, 1052
BsmI GAATGC 2 cut(s) 751, 1249
BsnI GGCC 3 cut(s) 206, 563, 906
Bso31I GGTCTC 1 cut(s) 1268
BsoBI CYCGRG 2 cut(s) 331, 415
Bsp1286I GDGCHC 2 cut(s) 295, 442
Bsp1407I TGTACA 1 cut(s) 480
Bsp143I GATC 6 cut(s) 9, 98, 859, 1096, 1198, 1387
Bsp1720I GCTNAGC 1 cut(s) 441
Bsp68I TCGCGA 1 cut(s) 254
BspANI GGCC 3 cut(s) 206, 563, 906
BspCNI CTCAG 7 cut(s) 281, 454, 797, 900, 1099, 1208, 1671
BspFNI CGCG 2 cut(s) 254, 386
BspHI TCATGA 1 cut(s) 12
BspLI GGNNCC 5 cut(s) 260, 294, 830, 1173, 1338
BspMAI CTGCAG 3 cut(s) 37, 1363, 1369
BspPI GGATC 2 cut(s) 867, 1193
BspQI GCTCTTC 1 cut(s) 165
BspT107I GGYRCC 2 cut(s) 292, 828
BspTNI GGTCTC 1 cut(s) 1268
BsrDI GCAATG 1 cut(s) 641
BsrGI TGTACA 1 cut(s) 480
BsrI ACTGG 4 cut(s) 1028, 1084, 1606, 1651
BssECI CCNNGG 4 cut(s) 105, 286, 466, 854
BssMI GATC 6 cut(s) 9, 98, 859, 1096, 1198, 1387
BssNAI GTATAC 1 cut(s) 800
Bst1107I GTATAC 1 cut(s) 800
Bst2UI CCWGG 3 cut(s) 738, 795, 855
Bst4CI ACNGT 5 cut(s) 525, 821, 924, 1414, 1673
Bst6I CTCTTC 3 cut(s) 165, 952, 1270
BstAUI TGTACA 1 cut(s) 480
BstBAI YACGTR 1 cut(s) 190
BstC8I GCNNGC 9 cut(s) 128, 224, 228, 248, 252, 490, 648, 904, 981
BstDEI CTNAG 8 cut(s) 268, 441, 784, 887, 1107, 1195, 1352, 1679
BstDSI CCRYGG 3 cut(s) 105, 286, 466
BstF5I GGATG 4 cut(s) 515, 1099, 1390, 1601
BstFNI CGCG 2 cut(s) 254, 386
BstH2I RGCGCY 2 cut(s) 329, 392
BstHHI GCGC 4 cut(s) 232, 328, 391, 1351
BstKTI GATC 6 cut(s) 12, 101, 862, 1099, 1201, 1390
BstMAI GTCTC 4 cut(s) 1243, 1268, 1316, 1616
BstMBI GATC 6 cut(s) 9, 98, 859, 1096, 1198, 1387
BstMCI CGRYCG 2 cut(s) 207, 823
BstMWI GCNNNNNNNGC 9 cut(s) 139, 323, 494, 569, 578, 1148, 1355, 1364, 1367
BstNI CCWGG 3 cut(s) 738, 795, 855
BstNSI RCATGY 1 cut(s) 481
BstSCI CCNGG 4 cut(s) 295, 736, 793, 853
BstSFI CTRYAG 3 cut(s) 33, 1359, 1365
BstSLI GKGCMC 1 cut(s) 295
BstUI CGCG 2 cut(s) 254, 386
BstV2I GAAGAC 2 cut(s) 387, 1468
BstX2I RGATCY 2 cut(s) 859, 1198
BstYI RGATCY 2 cut(s) 859, 1198
BstZ17I GTATAC 1 cut(s) 800
BstZI CGGCCG 1 cut(s) 204
BsuRI GGCC 3 cut(s) 206, 563, 906
BtgI CCRYGG 3 cut(s) 105, 286, 466
BtgZI GCGATG 3 cut(s) 624, 960, 1244
BtsCI GGATG 4 cut(s) 515, 1099, 1390, 1601
BtsI GCAGTG 2 cut(s) 87, 121
BtsIMutI CAGTG 5 cut(s) 87, 121, 248, 1613, 1676
BtuMI TCGCGA 1 cut(s) 254
Cac8I GCNNGC 9 cut(s) 128, 224, 228, 248, 252, 490, 648, 904, 981
CaiI CAGNNNCTG 1 cut(s) 1040
CciI TCATGA 1 cut(s) 12
CciNI GCGGCCGC 1 cut(s) 204
CfoI GCGC 4 cut(s) 232, 328, 391, 1351
Cfr13I GGNCC 3 cut(s) 259, 562, 791
CseI GACGC 2 cut(s) 392, 1412
Csp6I GTAC 5 cut(s) 283, 481, 829, 1516, 1624
CviQI GTAC 5 cut(s) 283, 481, 829, 1516, 1624
DdeI CTNAG 8 cut(s) 268, 441, 784, 887, 1107, 1195, 1352, 1679
DpnI GATC 6 cut(s) 11, 100, 861, 1098, 1200, 1389
DpnII GATC 6 cut(s) 9, 98, 859, 1096, 1198, 1387
DraI TTTAAA 1 cut(s) 1164
DraIII CACNNNGTG 1 cut(s) 299
EaeI YGGCCR 1 cut(s) 204
EagI CGGCCG 1 cut(s) 204
Eam1104I CTCTTC 3 cut(s) 165, 952, 1270
EarI CTCTTC 3 cut(s) 165, 952, 1270
EciI GGCGGA 2 cut(s) 548, 899
Ecl136II GAGCTC 1 cut(s) 440
EclXI CGGCCG 1 cut(s) 204
Eco24I GRGCYC 1 cut(s) 442
Eco31I GGTCTC 1 cut(s) 1268
Eco47I GGWCC 2 cut(s) 259, 791
Eco52I CGGCCG 1 cut(s) 204
Eco53kI GAGCTC 1 cut(s) 440
Eco57I CTGAAG 2 cut(s) 771, 933
Eco72I CACGTG 1 cut(s) 190
Eco88I CYCGRG 2 cut(s) 331, 415
EcoICRI GAGCTC 1 cut(s) 440
EcoRII CCWGG 3 cut(s) 736, 793, 853
EcoT38I GRGCYC 1 cut(s) 442
Esp3I CGTCTC 1 cut(s) 1316
FaqI GGGAC 3 cut(s) 152, 524, 1052
FbaI TGATCA 1 cut(s) 9
FblI GTMKAC 1 cut(s) 799
FokI GGATG 4 cut(s) 502, 1106, 1397, 1588
FriOI GRGCYC 1 cut(s) 442
FspBI CTAG 2 cut(s) 588, 833
GlaI GCGC 4 cut(s) 231, 327, 390, 1350
GsuI CTGGAG 3 cut(s) 1011, 1551, 1668
HaeII RGCGCY 2 cut(s) 329, 392
HaeIII GGCC 3 cut(s) 206, 563, 906
HapII CCGG 3 cut(s) 219, 262, 297
HgaI GACGC 2 cut(s) 392, 1412
HhaI GCGC 4 cut(s) 232, 328, 391, 1351
Hin6I GCGC 4 cut(s) 230, 326, 389, 1349
HinP1I GCGC 4 cut(s) 230, 326, 389, 1349
HincII GTYRAC 1 cut(s) 1441
HindII GTYRAC 1 cut(s) 1441
HinfI GANTC 5 cut(s) 512, 1278, 1451, 1501, 1564
HpaII CCGG 3 cut(s) 219, 262, 297
HphI GGTGA 1 cut(s) 93
Hpy166II GTNNAC 4 cut(s) 475, 800, 1441, 1676
Hpy188I TCNGA 6 cut(s) 271, 787, 1135, 1198, 1418, 1428
Hpy188III TCNNGA 6 cut(s) 13, 182, 253, 1458, 1568, 1633
Hpy8I GTNNAC 4 cut(s) 475, 800, 1441, 1676
Hpy99I CGWCG 3 cut(s) 49, 667, 1428
HpyAV CCTTC 3 cut(s) 78, 754, 1386
HpyCH4III ACNGT 5 cut(s) 525, 821, 924, 1414, 1673
HpyCH4IV ACGT 2 cut(s) 189, 1268
HpyF10VI GCNNNNNNNGC 9 cut(s) 139, 323, 494, 569, 578, 1148, 1355, 1364, 1367
HpyF3I CTNAG 8 cut(s) 268, 441, 784, 887, 1107, 1195, 1352, 1679
HpySE526I ACGT 2 cut(s) 189, 1268
HspAI GCGC 4 cut(s) 230, 326, 389, 1349
KpnI GGTACC 1 cut(s) 832
Ksp22I TGATCA 1 cut(s) 9
Kzo9I GATC 6 cut(s) 9, 98, 859, 1096, 1198, 1387
LguI GCTCTTC 1 cut(s) 165
LmnI GCTCC 2 cut(s) 375, 396
LweI GCATC 4 cut(s) 6, 601, 1216, 1286
MaeI CTAG 2 cut(s) 588, 833
MaeII ACGT 2 cut(s) 189, 1268
MaeIII GTNAC 2 cut(s) 809, 1004
MalI GATC 6 cut(s) 11, 100, 861, 1098, 1200, 1389
MboI GATC 6 cut(s) 9, 98, 859, 1096, 1198, 1387
MboII GAAGA 9 cut(s) 152, 392, 515, 587, 939, 1124, 1235, 1287, 1473
MflI RGATCY 2 cut(s) 859, 1198
MhlI GDGCHC 2 cut(s) 295, 442
MluCI AATT 6 cut(s) 237, 368, 1159, 1433, 1545, 1574
MlyI GAGTC 3 cut(s) 506, 1287, 1558
MmeI TCCRAC 2 cut(s) 1406, 1621
MseI TTAA 5 cut(s) 341, 770, 1163, 1181, 1548
MslI CAYNNNNRTG 4 cut(s) 153, 1062, 1383, 1606
MspA1I CMGCKG 2 cut(s) 1040, 1364
MspI CCGG 3 cut(s) 219, 262, 297
MspR9I CCNGG 4 cut(s) 297, 738, 795, 855
Mva1269I GAATGC 2 cut(s) 751, 1249
MvaI CCWGG 3 cut(s) 738, 795, 855
MvnI CGCG 2 cut(s) 254, 386
MwoI GCNNNNNNNGC 9 cut(s) 139, 323, 494, 569, 578, 1148, 1355, 1364, 1367
NciI CCSGG 1 cut(s) 297
NdeII GATC 6 cut(s) 9, 98, 859, 1096, 1198, 1387
NlaIV GGNNCC 5 cut(s) 260, 294, 830, 1173, 1338
NmuCI GTSAC 1 cut(s) 809
NotI GCGGCCGC 1 cut(s) 204
NruI TCGCGA 1 cut(s) 254
NspI RCATGY 1 cut(s) 481
PaeR7I CTCGAG 1 cut(s) 415
PagI TCATGA 1 cut(s) 12
PciI ACATGT 1 cut(s) 477
PciSI GCTCTTC 1 cut(s) 165
PctI GAATGC 2 cut(s) 751, 1249
PfeI GAWTC 2 cut(s) 1451, 1501
PflFI GACNNNGTC 1 cut(s) 1327
PflMI CCANNNNNTGG 1 cut(s) 1090
PleI GAGTC 3 cut(s) 506, 1286, 1558
PmaCI CACGTG 1 cut(s) 190
PmlI CACGTG 1 cut(s) 190
PpsI GAGTC 3 cut(s) 506, 1286, 1558
Ppu21I YACGTR 1 cut(s) 190
PscI ACATGT 1 cut(s) 477
Psp124BI GAGCTC 1 cut(s) 442
Psp6I CCWGG 3 cut(s) 736, 793, 853
PspCI CACGTG 1 cut(s) 190
PspGI CCWGG 3 cut(s) 736, 793, 853
PspN4I GGNNCC 5 cut(s) 260, 294, 830, 1173, 1338
PspPI GGNCC 3 cut(s) 259, 562, 791
PspXI VCTCGAGB 1 cut(s) 415
PstI CTGCAG 3 cut(s) 37, 1363, 1369
PstNI CAGNNNCTG 1 cut(s) 1040
PsuI RGATCY 2 cut(s) 859, 1198
PsyI GACNNNGTC 1 cut(s) 1327
PvuII CAGCTG 2 cut(s) 1040, 1364
RruI TCGCGA 1 cut(s) 254
RsaI GTAC 5 cut(s) 284, 482, 830, 1517, 1625
RsaNI GTAC 5 cut(s) 283, 481, 829, 1516, 1624
RseI CAYNNNNRTG 4 cut(s) 153, 1062, 1383, 1606
SacI GAGCTC 1 cut(s) 442
SapI GCTCTTC 1 cut(s) 165
SaqAI TTAA 5 cut(s) 341, 770, 1163, 1181, 1548
Sau3AI GATC 6 cut(s) 9, 98, 859, 1096, 1198, 1387
Sau96I GGNCC 3 cut(s) 259, 562, 791
SchI GAGTC 3 cut(s) 506, 1287, 1558
ScrFI CCNGG 4 cut(s) 297, 738, 795, 855
SduI GDGCHC 2 cut(s) 295, 442
SfaNI GCATC 4 cut(s) 6, 601, 1216, 1286
SfcI CTRYAG 3 cut(s) 33, 1359, 1365
Sfr274I CTCGAG 1 cut(s) 415
SinI GGWCC 2 cut(s) 259, 791
SlaI CTCGAG 1 cut(s) 415
SmiMI CAYNNNNRTG 4 cut(s) 153, 1062, 1383, 1606
SmlI CTYRAG 2 cut(s) 415, 1631
SmoI CTYRAG 2 cut(s) 415, 1631
Sse9I AATT 6 cut(s) 237, 368, 1159, 1433, 1545, 1574
SspMI CTAG 2 cut(s) 588, 833
SstI GAGCTC 1 cut(s) 442
StyD4I CCNGG 4 cut(s) 295, 736, 793, 853
TaaI ACNGT 5 cut(s) 525, 821, 924, 1414, 1673
TaiI ACGT 2 cut(s) 192, 1271
TaqI TCGA 6 cut(s) 183, 366, 416, 1153, 1221, 1390
TasI AATT 6 cut(s) 237, 368, 1159, 1433, 1545, 1574
TatI WGTACW 1 cut(s) 480
TauI GCSGC 6 cut(s) 206, 209, 359, 389, 405, 563
TfiI GAWTC 2 cut(s) 1451, 1501
Tru1I TTAA 5 cut(s) 341, 770, 1163, 1181, 1548
Tru9I TTAA 5 cut(s) 341, 770, 1163, 1181, 1548
TscAI CASTG 5 cut(s) 94, 128, 248, 1613, 1683
TseFI GTSAC 1 cut(s) 809
Tsp45I GTSAC 1 cut(s) 809
TspDTI ATGAA 5 cut(s) 758, 1242, 1391, 1443, 1548
TspRI CASTG 5 cut(s) 94, 128, 248, 1613, 1683
Tth111I GACNNNGTC 1 cut(s) 1327
Van91I CCANNNNNTGG 1 cut(s) 1090
VpaK11BI GGWCC 2 cut(s) 259, 791
XapI RAATTY 1 cut(s) 1574
XceI RCATGY 1 cut(s) 481
XcmI CCANNNNNNNNNTGG 2 cut(s) 899, 1613
XhoI CTCGAG 1 cut(s) 415
XmiI GTMKAC 1 cut(s) 799
XspI CTAG 2 cut(s) 588, 833
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.