pycom16g01490

Vacuolar fusion protein CCZ1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0005810
Physical Location & Seq
Forward (+)
925745 .. 929275
3531 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1587 bp
ATGGGCCTGTCGTCGGCCACTACTACGGCTACCGCTCCAGAAGGGGTGCAATTGTGCATATTCGATTTGAGGAGGGGTCAGAACGAAGGGCAGGAGTTGGACAAGATACTCTTCTTCTTTCCAGCTGATTTGCCCTTCTCCGCCCAGTTCTCTGTGATCGGGCTCAGTGAAGGACTCATCACATTTACAAGAATCTTCTCTCCTGAGGCTGCTTGCGAGGCCATTGAAGCAGAGAGGCATTCCCATGTCTTTCACGAGGCGGAACCCGATATCTGGATGGTCATGGTAGTGGAGAAAAACAAGGAGTTCGAACCAATTTGGAGAAGTGATGCATTGAGAAAGGTTCTCAAGGAAGTACATTCGCTTTTCGTGATGTTTCATGGATCGATAAGAGCGTTGCTTGATAAGGACCCCGGTGGGGGACTCACTCGTTCCCATCTGTACCATTTCGTCATGGACTATCTCGGTGCATTTCTAAAGCGGTCTCCATTCGATGAGTGCTGCTGGGATTTTCTGGTTGGGAAGAAACTGCTGCTACCCTCATTCCGTGACTCTTTAAAGGAGCGTGGAACTGTACAGATGCTTACTGTAGGGCGGGAAGCGGCAATCGAAGTTCAGTCGCTTGTCAGGGTACTTGAATCGTGTGCTGGGAACATGCCATGTCACTCACTAATCTTGTTTCAGGACCTCTTGGTGTCCACAACACTTTCTCCTGATGATACCGTAAACCTCTTTGCATATGCTGTTTTAAGGCTGACTCCCCGTGCTTTATCCTCTGGAGTAAGTTCCTGGTCCTATTTACGCAAGGGAGCTACATCGCATGTCTCCTCAAGTTCTAGCTTGGCCCGTCCTGGTTCTGTTCCAGAACACTTGCATGGCTCACTTGGCAACTCTCCTGCTGGAGATAACAGTTCTCGAGTCGTACGGCCCTTGCAGCCAGATAAGTGGTCCAAAGGAAAGGACGGTTTTCTTGTCAGTGATATTTGGGGTGCAGACACTAGTACTGGAACGTCTGCCACCCCAACAGTTTTGCTCCATCCAACAGATGAGAGAATGTATCTGTGTGCCCATCAGCATAAGAGCCTTACCTTAATTTTTCTTCTTCCTGTTTCTTCGGTACTAAATGGTGATCAAGGTGTTTCTGCAGTGAAGCAGCAAGTTCTTGAAAATGTATCCCTAAAGCTGTTGAAAGTTGAAGAGAAATTATCAAAAGGATGGGGTGGTGAGAATGCTTATCATGTTAGTGGATATCGGTATTTACTAGCAGATGGAGACAGAAATGTATCCAGGGCTTCTCCACCAGGAAAAGTCACAACCCTTACCAAGGGTTCCTTACTTGCCTTAAATAAGGTCAGAGAAGAAGTTGATTTGGACAAAAGTAGAGCGAAATTGGATAATGCTGGTCACGAGAAAGAATTGGAAATTAGCATCAGGGCGAAAAACAATGCTTGGGTTATCGCTCGGGTTACAAGAGGGAAGGAGCTTTATATGGTTCTAGAGAAAGCCAACGAAACACTTCTTTATGCCTCCGATGCTGTTGAGAAGTTCAGCAACAGGTACTGCAATGGAGCTTTCTCGTTGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0006810 GO:0008150 GO:0016192 GO:0031982 GO:0043226 GO:0043227 GO:0051179 GO:0051234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1712

Protein Analysis

529

Amino Acids

58.26

Weight (kDa)

6.77

Isoelectric Point (pI)

38.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Intu_longin_1 PF19031 19 - 128 4.7e-19 First Longin domain of INTU, CCZ1 and HPS4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 35
AciI CCGC 6 cut(s) 33, 141, 260, 481, 595, 602
AclWI GGATC 1 cut(s) 391
AcoI YGGCCR 1 cut(s) 15
AfaI GTAC 8 cut(s) 357, 443, 576, 633, 924, 1003, 1119, 1559
AfiI CCNNNNNNNGG 5 cut(s) 13, 273, 418, 419, 1324
AgsI TTSAA 6 cut(s) 227, 638, 1166, 1189, 1196, 1582
AhlI ACTAGT 1 cut(s) 998
AjnI CCWGG 4 cut(s) 788, 850, 1286, 1300
AluBI AGCT 6 cut(s) 125, 812, 840, 1183, 1483, 1571
AluI AGCT 6 cut(s) 125, 812, 840, 1183, 1483, 1571
Alw26I GTCTC 3 cut(s) 489, 829, 1266
AlwI GGATC 1 cut(s) 391
AlwNI CAGNNNCTG 1 cut(s) 1560
Ama87I CYCGRG 2 cut(s) 915, 1461
AoxI GGCC 5 cut(s) 4, 15, 219, 843, 926
ApeKI GCWGC 5 cut(s) 209, 501, 532, 934, 1153
Asp700I GAANNNNTTC 1 cut(s) 1515
AspS9I GGNCC 7 cut(s) 4, 409, 685, 792, 844, 927, 948
AsuC2I CCSGG 1 cut(s) 414
AsuHPI GGTGA 2 cut(s) 1139, 1235
AsuII TTCGAA 1 cut(s) 309
AvaI CYCGRG 2 cut(s) 915, 1461
AvaII GGWCC 4 cut(s) 409, 685, 792, 948
AxyI CCTNAGG 1 cut(s) 204
BaeGI GKGCMC 1 cut(s) 1069
BanII GRGCYC 1 cut(s) 165
BauI CACGAG 2 cut(s) 254, 1406
BbvI GCAGC 5 cut(s) 196, 488, 519, 946, 1165
BccI CCATC 6 cut(s) 271, 444, 1044, 1077, 1209, 1262
BceAI ACGGC 2 cut(s) 42, 941
BciT130I CCWGG 4 cut(s) 790, 852, 1288, 1302
BciVI GTATCC 2 cut(s) 1183, 1294
BclI TGATCA 1 cut(s) 1129
BcnI CCSGG 1 cut(s) 414
BcoDI GTCTC 3 cut(s) 489, 829, 1266
BcuI ACTAGT 1 cut(s) 998
BfaI CTAG 4 cut(s) 837, 999, 1262, 1496
BfmI CTRYAG 2 cut(s) 588, 1143
BfuI GTATCC 2 cut(s) 1183, 1294
BisI GCNGC 6 cut(s) 210, 502, 533, 603, 935, 1154
BlsI GCNGC 6 cut(s) 211, 503, 534, 604, 936, 1155
BmcAI AGTACT 1 cut(s) 1003
Bme1390I CCNGG 5 cut(s) 414, 790, 852, 1288, 1302
Bme18I GGWCC 4 cut(s) 409, 685, 792, 948
BmeT110I CYCGRG 2 cut(s) 915, 1461
BmgT120I GGNCC 7 cut(s) 4, 409, 685, 792, 844, 927, 948
BmiI GGNNCC 3 cut(s) 264, 411, 1330
BmrFI CCNGG 5 cut(s) 414, 790, 852, 1288, 1302
BmrI ACTGGG 1 cut(s) 139
BmsI GCATC 4 cut(s) 319, 570, 1437, 1522
BmuI ACTGGG 1 cut(s) 139
BpmI CTGGAG 3 cut(s) 21, 798, 921
Bpu14I TTCGAA 1 cut(s) 309
BpuEI CTTGAG 2 cut(s) 332, 814
BpuMI CCSGG 1 cut(s) 414
Bsa29I ATCGAT 1 cut(s) 386
BsaI GGTCTC 1 cut(s) 489
BsaJI CCNNGG 3 cut(s) 412, 1287, 1323
BsaXI ACNNNNNCTCC 2 cut(s) 694, 724
Bsc4I CCNNNNNNNGG 5 cut(s) 13, 273, 418, 419, 1324
Bse1I ACTGG 2 cut(s) 145, 1009
Bse21I CCTNAGG 1 cut(s) 204
Bse3DI GCAATG 1 cut(s) 1570
BseBI CCWGG 4 cut(s) 790, 852, 1288, 1302
BseCI ATCGAT 1 cut(s) 386
BseDI CCNNGG 3 cut(s) 412, 1287, 1323
BseGI GGATG 3 cut(s) 282, 1036, 1220
BseLI CCNNNNNNNGG 5 cut(s) 13, 273, 418, 419, 1324
BseMI GCAATG 1 cut(s) 1570
BseMII CTCAG 2 cut(s) 178, 195
BseNI ACTGG 2 cut(s) 145, 1009
BseRI GAGGAG 2 cut(s) 85, 817
BseSI GKGCMC 1 cut(s) 1069
BseXI GCAGC 5 cut(s) 196, 488, 519, 946, 1165
BseYI CCCAGC 2 cut(s) 504, 647
BsgI GTGCAG 1 cut(s) 1011
BshFI GGCC 5 cut(s) 6, 17, 221, 845, 928
BshVI ATCGAT 1 cut(s) 386
BsiHKCI CYCGRG 2 cut(s) 915, 1461
BsiSI CCGG 1 cut(s) 414
BsiWI CGTACG 1 cut(s) 922
BslFI GGGAC 1 cut(s) 435
BslI CCNNNNNNNGG 5 cut(s) 13, 273, 418, 419, 1324
BsmAI GTCTC 3 cut(s) 489, 829, 1266
BsmFI GGGAC 1 cut(s) 435
BsmI GAATGC 2 cut(s) 238, 1234
BsnI GGCC 5 cut(s) 6, 17, 221, 845, 928
Bso31I GGTCTC 1 cut(s) 489
BsoBI CYCGRG 2 cut(s) 915, 1461
Bsp119I TTCGAA 1 cut(s) 309
Bsp1286I GDGCHC 2 cut(s) 165, 1069
Bsp1407I TGTACA 1 cut(s) 574
Bsp143I GATC 3 cut(s) 156, 383, 1129
BspACI CCGC 6 cut(s) 33, 141, 260, 481, 595, 602
BspANI GGCC 5 cut(s) 6, 17, 221, 845, 928
BspCNI CTCAG 2 cut(s) 177, 196
BspDI ATCGAT 1 cut(s) 386
BspLI GGNNCC 3 cut(s) 264, 411, 1330
BspMAI CTGCAG 1 cut(s) 1147
BspPI GGATC 1 cut(s) 391
BspT104I TTCGAA 1 cut(s) 309
BspTNI GGTCTC 1 cut(s) 489
BsrBI CCGCTC 1 cut(s) 35
BsrDI GCAATG 1 cut(s) 1570
BsrGI TGTACA 1 cut(s) 574
BsrI ACTGG 2 cut(s) 145, 1009
BssECI CCNNGG 3 cut(s) 412, 1287, 1323
BssMI GATC 3 cut(s) 156, 383, 1129
BssSI CACGAG 2 cut(s) 254, 1406
BssT1I CCWWGG 1 cut(s) 1323
Bst2BI CACGAG 2 cut(s) 254, 1406
Bst2UI CCWGG 4 cut(s) 790, 852, 1288, 1302
Bst4CI ACNGT 6 cut(s) 574, 589, 724, 911, 965, 1027
Bst6I CTCTTC 2 cut(s) 116, 1191
BstAUI TGTACA 1 cut(s) 574
BstBI TTCGAA 1 cut(s) 309
BstC8I GCNNGC 1 cut(s) 214
BstDEI CTNAG 2 cut(s) 164, 204
BstENI CCTNNNNNAGG 1 cut(s) 1322
BstF5I GGATG 3 cut(s) 282, 1036, 1220
BstKTI GATC 3 cut(s) 159, 386, 1132
BstMAI GTCTC 3 cut(s) 489, 829, 1266
BstMBI GATC 3 cut(s) 156, 383, 1129
BstMWI GCNNNNNNNGC 5 cut(s) 218, 227, 885, 934, 1532
BstNI CCWGG 4 cut(s) 790, 852, 1288, 1302
BstNSI RCATGY 2 cut(s) 658, 824
BstSCI CCNGG 5 cut(s) 412, 788, 850, 1286, 1300
BstSFI CTRYAG 2 cut(s) 588, 1143
BstSLI GKGCMC 1 cut(s) 1069
BstV1I GCAGC 5 cut(s) 196, 488, 519, 946, 1165
BstXI CCANNNNNNTGG 1 cut(s) 945
Bsu15I ATCGAT 1 cut(s) 386
Bsu36I CCTNAGG 1 cut(s) 204
BsuI GTATCC 2 cut(s) 1183, 1294
BsuRI GGCC 5 cut(s) 6, 17, 221, 845, 928
BsuTUI ATCGAT 1 cut(s) 386
BtgZI GCGATG 1 cut(s) 801
BtsCI GGATG 3 cut(s) 282, 1036, 1220
BtsI GCAGTG 1 cut(s) 1152
BtsIMutI CAGTG 3 cut(s) 172, 982, 1152
Cac8I GCNNGC 1 cut(s) 214
CaiI CAGNNNCTG 1 cut(s) 1560
Cfr13I GGNCC 7 cut(s) 4, 409, 685, 792, 844, 927, 948
ClaI ATCGAT 1 cut(s) 386
Csp6I GTAC 8 cut(s) 356, 442, 575, 632, 923, 1002, 1118, 1558
CviAII CATG 9 cut(s) 245, 283, 380, 454, 655, 660, 821, 875, 1238
CviQI GTAC 8 cut(s) 356, 442, 575, 632, 923, 1002, 1118, 1558
DdeI CTNAG 2 cut(s) 164, 204
DpnI GATC 3 cut(s) 158, 385, 1131
DpnII GATC 3 cut(s) 156, 383, 1129
DraI TTTAAA 1 cut(s) 558
EaeI YGGCCR 1 cut(s) 15
Eam1104I CTCTTC 2 cut(s) 116, 1191
EarI CTCTTC 2 cut(s) 116, 1191
EciI GGCGGA 2 cut(s) 130, 275
Eco130I CCWWGG 1 cut(s) 1323
Eco24I GRGCYC 1 cut(s) 165
Eco31I GGTCTC 1 cut(s) 489
Eco32I GATATC 2 cut(s) 271, 1250
Eco47I GGWCC 4 cut(s) 409, 685, 792, 948
Eco81I CCTNAGG 1 cut(s) 204
Eco88I CYCGRG 2 cut(s) 915, 1461
EcoNI CCTNNNNNAGG 1 cut(s) 1322
EcoO109I RGGNCCY 2 cut(s) 409, 685
EcoRII CCWGG 4 cut(s) 788, 850, 1286, 1300
EcoRV GATATC 2 cut(s) 271, 1250
EcoT14I CCWWGG 1 cut(s) 1323
EcoT22I ATGCAT 1 cut(s) 334
EcoT38I GRGCYC 1 cut(s) 165
ErhI CCWWGG 1 cut(s) 1323
FaeI CATG 9 cut(s) 248, 286, 383, 457, 658, 663, 824, 878, 1241
FalI AAGNNNNNCTT 4 cut(s) 95, 127, 1316, 1348
FaqI GGGAC 1 cut(s) 435
FatI CATG 9 cut(s) 244, 282, 379, 453, 654, 659, 820, 874, 1237
FauI CCCGC 1 cut(s) 588
FauNDI CATATG 1 cut(s) 739
FbaI TGATCA 1 cut(s) 1129
Fnu4HI GCNGC 6 cut(s) 210, 502, 533, 603, 935, 1154
FokI GGATG 3 cut(s) 289, 1023, 1227
FriOI GRGCYC 1 cut(s) 165
Fsp4HI GCNGC 6 cut(s) 210, 502, 533, 603, 935, 1154
FspBI CTAG 4 cut(s) 837, 999, 1262, 1496
GluI GCNGC 6 cut(s) 210, 502, 533, 603, 935, 1154
GsaI CCCAGC 2 cut(s) 508, 651
GsuI CTGGAG 3 cut(s) 21, 798, 921
HaeIII GGCC 5 cut(s) 6, 17, 221, 845, 928
HapII CCGG 1 cut(s) 414
Hin1II CATG 9 cut(s) 248, 286, 383, 457, 658, 663, 824, 878, 1241
HinfI GANTC 7 cut(s) 174, 192, 423, 551, 638, 757, 918
HpaII CCGG 1 cut(s) 414
HphI GGTGA 2 cut(s) 1139, 1235
Hpy166II GTNNAC 2 cut(s) 699, 727
Hpy188I TCNGA 3 cut(s) 81, 1355, 1531
Hpy8I GTNNAC 2 cut(s) 699, 727
Hpy99I CGWCG 1 cut(s) 16
HpyAV CCTTC 5 cut(s) 35, 80, 145, 164, 1471
HpyCH4III ACNGT 6 cut(s) 574, 589, 724, 911, 965, 1027
HpyCH4IV ACGT 1 cut(s) 1010
HpyF10VI GCNNNNNNNGC 5 cut(s) 218, 227, 885, 934, 1532
HpyF3I CTNAG 2 cut(s) 164, 204
HpySE526I ACGT 1 cut(s) 1010
Hsp92II CATG 9 cut(s) 248, 286, 383, 457, 658, 663, 824, 878, 1241
Ksp22I TGATCA 1 cut(s) 1129
Kzo9I GATC 3 cut(s) 156, 383, 1129
LmnI GCTCC 6 cut(s) 40, 562, 809, 1038, 1480, 1568
Lsp1109I GCAGC 5 cut(s) 196, 488, 519, 946, 1165
LweI GCATC 4 cut(s) 319, 570, 1437, 1522
MaeI CTAG 4 cut(s) 837, 999, 1262, 1496
MaeII ACGT 1 cut(s) 1010
MaeIII GTNAC 5 cut(s) 548, 662, 1309, 1403, 1465
MalI GATC 3 cut(s) 158, 385, 1131
MbiI CCGCTC 1 cut(s) 35
MboI GATC 3 cut(s) 156, 383, 1129
MboII GAAGA 9 cut(s) 103, 106, 187, 535, 1091, 1094, 1104, 1208, 1370
MfeI CAATTG 1 cut(s) 50
MhlI GDGCHC 2 cut(s) 165, 1069
MluCI AATT 8 cut(s) 50, 315, 1092, 1202, 1388, 1415, 1422, 1582
MlyI GAGTC 5 cut(s) 168, 417, 545, 751, 927
MmeI TCCRAC 2 cut(s) 78, 1064
Mph1103I ATGCAT 1 cut(s) 334
MroXI GAANNNNTTC 1 cut(s) 1515
MseI TTAA 4 cut(s) 557, 749, 1091, 1343
MslI CAYNNNNRTG 4 cut(s) 243, 287, 873, 1242
MspA1I CMGCKG 1 cut(s) 125
MspI CCGG 1 cut(s) 414
MspR9I CCNGG 5 cut(s) 414, 790, 852, 1288, 1302
MunI CAATTG 1 cut(s) 50
Mva1269I GAATGC 2 cut(s) 238, 1234
MvaI CCWGG 4 cut(s) 790, 852, 1288, 1302
MwoI GCNNNNNNNGC 5 cut(s) 218, 227, 885, 934, 1532
NciI CCSGG 1 cut(s) 414
NdeI CATATG 1 cut(s) 739
NdeII GATC 3 cut(s) 156, 383, 1129
NlaIII CATG 9 cut(s) 248, 286, 383, 457, 658, 663, 824, 878, 1241
NlaIV GGNNCC 3 cut(s) 264, 411, 1330
NmuCI GTSAC 4 cut(s) 548, 662, 1309, 1403
NsiI ATGCAT 1 cut(s) 334
NspI RCATGY 2 cut(s) 658, 824
NspV TTCGAA 1 cut(s) 309
PaeR7I CTCGAG 1 cut(s) 915
PcsI WCGNNNNNNNCGW 1 cut(s) 392
PctI GAATGC 2 cut(s) 238, 1234
PdmI GAANNNNTTC 1 cut(s) 1515
PfeI GAWTC 2 cut(s) 192, 638
Pfl23II CGTACG 1 cut(s) 922
PkrI GCNGC 6 cut(s) 211, 503, 534, 604, 936, 1155
PleI GAGTC 5 cut(s) 168, 417, 545, 751, 926
PpsI GAGTC 5 cut(s) 168, 417, 545, 751, 926
PpuMI RGGWCCY 2 cut(s) 409, 685
Psp5II RGGWCCY 2 cut(s) 409, 685
Psp6I CCWGG 4 cut(s) 788, 850, 1286, 1300
PspFI CCCAGC 2 cut(s) 504, 647
PspGI CCWGG 4 cut(s) 788, 850, 1286, 1300
PspLI CGTACG 1 cut(s) 922
PspN4I GGNNCC 3 cut(s) 264, 411, 1330
PspPI GGNCC 7 cut(s) 4, 409, 685, 792, 844, 927, 948
PspPPI RGGWCCY 2 cut(s) 409, 685
PstI CTGCAG 1 cut(s) 1147
PstNI CAGNNNCTG 1 cut(s) 1560
PvuII CAGCTG 1 cut(s) 125
RsaI GTAC 8 cut(s) 357, 443, 576, 633, 924, 1003, 1119, 1559
RsaNI GTAC 8 cut(s) 356, 442, 575, 632, 923, 1002, 1118, 1558
RseI CAYNNNNRTG 4 cut(s) 243, 287, 873, 1242
SaqAI TTAA 4 cut(s) 557, 749, 1091, 1343
SatI GCNGC 6 cut(s) 210, 502, 533, 603, 935, 1154
Sau3AI GATC 3 cut(s) 156, 383, 1129
Sau96I GGNCC 7 cut(s) 4, 409, 685, 792, 844, 927, 948
ScaI AGTACT 1 cut(s) 1003
SchI GAGTC 5 cut(s) 168, 417, 545, 751, 927
ScrFI CCNGG 5 cut(s) 414, 790, 852, 1288, 1302
SduI GDGCHC 2 cut(s) 165, 1069
SfaNI GCATC 4 cut(s) 319, 570, 1437, 1522
SfcI CTRYAG 2 cut(s) 588, 1143
Sfr274I CTCGAG 1 cut(s) 915
SfuI TTCGAA 1 cut(s) 309
SinI GGWCC 4 cut(s) 409, 685, 792, 948
SlaI CTCGAG 1 cut(s) 915
SmiMI CAYNNNNRTG 4 cut(s) 243, 287, 873, 1242
SmlI CTYRAG 3 cut(s) 347, 829, 915
SmoI CTYRAG 3 cut(s) 347, 829, 915
SpeI ACTAGT 1 cut(s) 998
Sse9I AATT 8 cut(s) 50, 315, 1092, 1202, 1388, 1415, 1422, 1582
SsiI CCGC 6 cut(s) 33, 141, 260, 481, 595, 602
SspMI CTAG 4 cut(s) 837, 999, 1262, 1496
StyD4I CCNGG 5 cut(s) 412, 788, 850, 1286, 1300
StyI CCWWGG 1 cut(s) 1323
TaaI ACNGT 6 cut(s) 574, 589, 724, 911, 965, 1027
TaiI ACGT 1 cut(s) 1013
TaqI TCGA 6 cut(s) 63, 309, 386, 492, 609, 916
TasI AATT 8 cut(s) 50, 315, 1092, 1202, 1388, 1415, 1422, 1582
TatI WGTACW 3 cut(s) 355, 574, 1001
TauI GCSGC 1 cut(s) 605
TfiI GAWTC 2 cut(s) 192, 638
Tru1I TTAA 4 cut(s) 557, 749, 1091, 1343
Tru9I TTAA 4 cut(s) 557, 749, 1091, 1343
TscAI CASTG 3 cut(s) 172, 982, 1152
TseFI GTSAC 4 cut(s) 548, 662, 1309, 1403
TseI GCWGC 5 cut(s) 209, 501, 532, 934, 1153
Tsp45I GTSAC 4 cut(s) 548, 662, 1309, 1403
TspDTI ATGAA 1 cut(s) 368
TspGWI ACGGA 1 cut(s) 536
TspRI CASTG 3 cut(s) 172, 982, 1152
VpaK11BI GGWCC 4 cut(s) 409, 685, 792, 948
XagI CCTNNNNNAGG 1 cut(s) 1322
XbaI TCTAGA 1 cut(s) 1495
XceI RCATGY 2 cut(s) 658, 824
XhoI CTCGAG 1 cut(s) 915
XmnI GAANNNNTTC 1 cut(s) 1515
XspI CTAG 4 cut(s) 837, 999, 1262, 1496
ZrmI AGTACT 1 cut(s) 1003
Zsp2I ATGCAT 1 cut(s) 334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.