pycom16g02060

Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain III

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0008691
Physical Location & Seq
Forward (+)
1305966 .. 1308708
2743 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1857 bp
ATGGCTTCCACTTCAACTTCTTCAACAATTTATTTGCAAACCAATGCTCAAAAACTATCTTTCCCCTCAAAGCCGGCAACCAAAACATCCCACACAAACCTTAGCTTTCCGTACTCATTGCGATTCGCTACTAAATTTGTACGTGTGAAGTCTTCTAGCACTGCCTACAATGAAGTTGTGGATGAAGAGATGGACAGAATCAGAAGGCTCCAGAACGGGTCTGATGTTCGCGGGGTAGCCTTGGAAGGCGAGAAGGGGCGGATTGTGGACCTAACACCACCGGCTGTGGAGGCCATTGCGGAGAGTTTTGGTGAGTGGGTTATCGACGGCTTGGAGATGGAGCAAGGACAACTTGTGGAGAAAGTTAGGGTGTCCCTTGGCAAGGACCCAAGGATTTCAGGACCAAATTTGAGTGTAGCTGTATTTTCGGGACTAGGTCGTGCAGGTTGTTTGGTATTCGATATGGGACTTGCAACTACACCGGCCTGCTTCATGAGTACAATTTTGCCTCCATTTTCCTACGACGCTTCAATCATGATGACCGCATCTCACTTACCCTACACCCGAAATGGTCTCAAATTTTTCACCAAGAAAGGAGGACTAAGCTCGCCAGAAGTCGAAGACATATGCAATAGAGCAGCCCGAAAGTATGCCAATAGGCTCGCCAAAGTGTCAACATTGCTCAATGTCCCTCCATCAAGAGTTGATTTCATGAGCTCGTACGCAAAGCACCTCCGAGACATTATCAAGGAGAGAGTGAACCATCCGTTGCATTACGAAACTCCACTCCAAGGATTCCAGATAATAGTGAATGCAGGTAATGGATCAGGAGGCTTTTTCACATGGGATGTGTTGGATAAACTTGGGGCAGACACCTTTGGCTCTCTATACCTTAATCCAGACGGAATGTTTCCGAACCACATTCCCAACCCTGAGGACAAAACCGCTATGGCGGTAACCAGAGCTGCAGTGCTAGAAAACTCTGCTGATCTTGGGATTGTATTTGACACTGATGTGGATCGCAGCGGCATTGTGGATAAAAAGGGCAACCCCATCAATGGCGACAAGCTCATTGCTTTGATTTCTGCTATCGTATTGAGGGAACACCCTGGAACCACCATAGTGACCGATGCGCGTACAAGTATGGCGCTTACAAGATTCATCACGAATCGGGGGGGTCATCATTGCTTATATCGTGTTGGTTATCGAAATGTGATTGATAAGGGAGTGCAGCTTAACAATGAAGGCATTGAAACTCATCTCATGATGGAAACATCTGGACACGGTGCTCTTAAAGAGAACCATTTCCTTGATGATGGAGCTTACATGGTGGTAAAGATCATCATCGATATGGTGCGAATGAAGCTTGCAGGATCAGACGAAGGGGTTGGAAACATCATAAAAGACCTTGAAGAACCGTCAGAGTCCATAGAGCTGAGGATGAATGTAATTTCTGAGCCTAGATATGCAAAAGAAAAGGCAGTGGAGGCCATTGAAACGTTCCGAGAGTACGTTGAGGAGGGGCGGCTTGAAGGGTGGGAGTTAGATTCGTGCGGAGATTGTTGGGTCAGCGAGGGCTGTTTGGTAGACTCAAATGATGCGTCAGCTGCTGCCGTTGACGCACACATGTACAGAGCAAAAGTATCAGATGAGGAACATGGACAGCATGGTTGGGTGCACATTAGGCAGAGCATTCACAACCCGAATATTGCAGTGAACATGCAATCAACTGCGCCTGGCTGTTGCCGAACAATGACAAGAGTTCTTCTAGATAAATTTCTCATAGCAAGCGGAATGGATAGAATTCTTGACATTAACCAGATTGATAAGTATGCCAAGAGTGGAATTGTAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004615 GO:0005975 GO:0008150 GO:0008152 GO:0016853 GO:0016866 GO:0016868 GO:0044238 GO:0071704
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PGM_PMM_I PGM_PMM_II PGM_PMM_III

Protein Analysis

619

Amino Acids

67.9

Weight (kDa)

5.84

Isoelectric Point (pI)

30.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGM_PMM_I PF02878 70 - 214 1.5e-21 Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain I
PGM_PMM_II PF02879 241 - 349 9e-20 Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain II
PGM_PMM_III PF02880 353 - 448 1.1e-10 Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 434, 806
AccI GTMKAC 1 cut(s) 1587
AccII CGCG 2 cut(s) 231, 1135
AclI AACGTT 1 cut(s) 1499
AclWI GGATC 3 cut(s) 832, 1026, 1381
AcsI RAATTY 5 cut(s) 134, 406, 578, 1775, 1803
AfaI GTAC 7 cut(s) 113, 141, 499, 722, 1138, 1511, 1631
AfiI CCNNNNNNNGG 3 cut(s) 382, 791, 1058
AflIII ACRYGT 2 cut(s) 142, 1626
AgsI TTSAA 7 cut(s) 15, 24, 531, 1253, 1412, 1496, 1532
AjnI CCWGG 2 cut(s) 1108, 1735
AleI CACNNNNGTG 2 cut(s) 1013, 1121
Alw21I GWGCWC 3 cut(s) 719, 1291, 1680
Alw26I GTCTC 2 cut(s) 578, 732
Alw44I GTGCAC 1 cut(s) 1676
AlwI GGATC 3 cut(s) 832, 1026, 1381
AlwNI CAGNNNCTG 1 cut(s) 1610
AoxI GGCC 3 cut(s) 291, 483, 1488
ApaLI GTGCAC 1 cut(s) 1676
ApeKI GCWGC 6 cut(s) 638, 965, 1023, 1231, 1607, 1610
ApoI RAATTY 5 cut(s) 134, 406, 578, 1775, 1803
ArsI GACNNNNNNTTYG 2 cut(s) 1586, 1618
Asp700I GAANNNNTTC 1 cut(s) 1304
AspLEI GCGC 3 cut(s) 1135, 1150, 1735
AspS9I GGNCC 3 cut(s) 268, 385, 401
AsuHPI GGTGA 2 cut(s) 323, 577
AvaII GGWCC 3 cut(s) 268, 385, 401
AxyI CCTNAGG 1 cut(s) 933
BaeGI GKGCMC 1 cut(s) 1680
BanII GRGCYC 1 cut(s) 719
BbsI GAAGAC 2 cut(s) 144, 627
Bbv12I GWGCWC 3 cut(s) 719, 1291, 1680
BbvCI CCTCAGC 1 cut(s) 1436
BbvI GCAGC 6 cut(s) 650, 952, 1035, 1243, 1594, 1597
BccI CCATC 7 cut(s) 184, 331, 703, 771, 1061, 1261, 1310
BceAI ACGGC 2 cut(s) 343, 1598
BciT130I CCWGG 2 cut(s) 1110, 1737
BcoDI GTCTC 2 cut(s) 578, 732
BfaI CTAG 5 cut(s) 156, 434, 974, 1461, 1769
BfmI CTRYAG 1 cut(s) 966
BfoI RGCGCY 1 cut(s) 1151
BfuAI ACCTGC 2 cut(s) 434, 806
BisI GCNGC 8 cut(s) 639, 966, 1024, 1027, 1232, 1526, 1608, 1611
BlsI GCNGC 8 cut(s) 640, 967, 1025, 1028, 1233, 1527, 1609, 1612
Bme1390I CCNGG 2 cut(s) 1110, 1737
Bme18I GGWCC 3 cut(s) 268, 385, 401
BmgT120I GGNCC 3 cut(s) 268, 385, 401
BmiI GGNNCC 3 cut(s) 209, 387, 1114
BmrFI CCNGG 2 cut(s) 1110, 1737
BmsI GCATC 3 cut(s) 554, 1120, 1588
BpiI GAAGAC 2 cut(s) 144, 627
BpmI CTGGAG 1 cut(s) 194
Bpu10I CCTNAGC 2 cut(s) 101, 1436
Bsa29I ATCGAT 1 cut(s) 1347
BsaAI YACGTR 1 cut(s) 143
BsaBI GATNNNNATC 2 cut(s) 1017, 1343
BsaI GGTCTC 1 cut(s) 578
BsaJI CCNNGG 5 cut(s) 240, 376, 389, 790, 1108
Bsc4I CCNNNNNNNGG 3 cut(s) 382, 791, 1058
Bse118I RCCGGY 3 cut(s) 73, 280, 481
Bse21I CCTNAGG 1 cut(s) 933
Bse3DI GCAATG 5 cut(s) 116, 294, 677, 1071, 1183
Bse8I GATNNNNATC 2 cut(s) 1017, 1343
BseBI CCWGG 2 cut(s) 1110, 1737
BseCI ATCGAT 1 cut(s) 1347
BseDI CCNNGG 5 cut(s) 240, 376, 389, 790, 1108
BseGI GGATG 5 cut(s) 86, 187, 763, 853, 1446
BseJI GATNNNNATC 2 cut(s) 1017, 1343
BseLI CCNNNNNNNGG 3 cut(s) 382, 791, 1058
BseMI GCAATG 5 cut(s) 116, 294, 677, 1071, 1183
BseMII CTCAG 3 cut(s) 924, 1427, 1446
BseRI GAGGAG 1 cut(s) 1532
BseSI GKGCMC 1 cut(s) 1680
BseXI GCAGC 6 cut(s) 650, 952, 1035, 1243, 1594, 1597
BsgI GTGCAG 2 cut(s) 462, 1250
Bsh1236I CGCG 2 cut(s) 231, 1135
BshFI GGCC 3 cut(s) 293, 485, 1490
BshVI ATCGAT 1 cut(s) 1347
BsiHKAI GWGCWC 3 cut(s) 719, 1291, 1680
BsiSI CCGG 3 cut(s) 74, 281, 482
BsiWI CGTACG 1 cut(s) 720
BslFI GGGAC 4 cut(s) 358, 444, 480, 674
BslI CCNNNNNNNGG 3 cut(s) 382, 791, 1058
BsmAI GTCTC 2 cut(s) 578, 732
BsmFI GGGAC 4 cut(s) 358, 444, 480, 674
BsmI GAATGC 2 cut(s) 817, 1692
BsnI GGCC 3 cut(s) 293, 485, 1490
Bso31I GGTCTC 1 cut(s) 578
Bsp1286I GDGCHC 3 cut(s) 719, 1291, 1680
Bsp1407I TGTACA 1 cut(s) 1629
Bsp143I GATC 5 cut(s) 824, 988, 1018, 1338, 1373
BspANI GGCC 3 cut(s) 293, 485, 1490
BspCNI CTCAG 3 cut(s) 925, 1428, 1447
BspDI ATCGAT 1 cut(s) 1347
BspFNI CGCG 2 cut(s) 231, 1135
BspHI TCATGA 4 cut(s) 492, 534, 711, 1263
BspLI GGNNCC 3 cut(s) 209, 387, 1114
BspMAI CTGCAG 1 cut(s) 970
BspMI ACCTGC 2 cut(s) 434, 806
BspPI GGATC 3 cut(s) 832, 1026, 1381
BspTNI GGTCTC 1 cut(s) 578
BsrDI GCAATG 5 cut(s) 116, 294, 677, 1071, 1183
BsrFI RCCGGY 3 cut(s) 73, 280, 481
BsrGI TGTACA 1 cut(s) 1629
BssAI RCCGGY 3 cut(s) 73, 280, 481
BssECI CCNNGG 5 cut(s) 240, 376, 389, 790, 1108
BssMI GATC 5 cut(s) 824, 988, 1018, 1338, 1373
BssT1I CCWWGG 4 cut(s) 240, 376, 389, 790
Bst2UI CCWGG 2 cut(s) 1110, 1737
Bst4CI ACNGT 2 cut(s) 1286, 1419
Bst6I CTCTTC 1 cut(s) 180
BstAUI TGTACA 1 cut(s) 1629
BstBAI YACGTR 1 cut(s) 143
BstC8I GCNNGC 6 cut(s) 75, 487, 608, 663, 1368, 1789
BstDEI CTNAG 5 cut(s) 101, 602, 933, 1436, 1455
BstEII GGTNACC 1 cut(s) 955
BstENI CCTNNNNNAGG 1 cut(s) 380
BstF5I GGATG 5 cut(s) 86, 187, 763, 853, 1446
BstFNI CGCG 2 cut(s) 231, 1135
BstH2I RGCGCY 1 cut(s) 1151
BstHHI GCGC 3 cut(s) 1135, 1150, 1735
BstKTI GATC 5 cut(s) 827, 991, 1021, 1341, 1376
BstMAI GTCTC 2 cut(s) 578, 732
BstMBI GATC 5 cut(s) 824, 988, 1018, 1338, 1373
BstMWI GCNNNNNNNGC 6 cut(s) 290, 1363, 1487, 1607, 1619, 1684
BstNI CCWGG 2 cut(s) 1110, 1737
BstNSI RCATGY 2 cut(s) 1630, 1723
BstPI GGTNACC 1 cut(s) 955
BstSCI CCNGG 2 cut(s) 1108, 1735
BstSFI CTRYAG 1 cut(s) 966
BstSLI GKGCMC 1 cut(s) 1680
BstUI CGCG 2 cut(s) 231, 1135
BstV1I GCAGC 6 cut(s) 650, 952, 1035, 1243, 1594, 1597
BstV2I GAAGAC 2 cut(s) 144, 627
Bsu15I ATCGAT 1 cut(s) 1347
Bsu36I CCTNAGG 1 cut(s) 933
BsuRI GGCC 3 cut(s) 293, 485, 1490
BsuTUI ATCGAT 1 cut(s) 1347
BtsCI GGATG 5 cut(s) 86, 187, 763, 853, 1446
BtsI GCAGTG 4 cut(s) 159, 975, 1488, 1719
BtsIMutI CAGTG 5 cut(s) 159, 975, 1008, 1488, 1719
BveI ACCTGC 2 cut(s) 434, 806
Cac8I GCNNGC 6 cut(s) 75, 487, 608, 663, 1368, 1789
CaiI CAGNNNCTG 1 cut(s) 1610
CciI TCATGA 4 cut(s) 492, 534, 711, 1263
CfoI GCGC 3 cut(s) 1135, 1150, 1735
Cfr10I RCCGGY 3 cut(s) 73, 280, 481
Cfr13I GGNCC 3 cut(s) 268, 385, 401
ClaI ATCGAT 1 cut(s) 1347
CseI GACGC 3 cut(s) 533, 1590, 1628
Csp6I GTAC 7 cut(s) 112, 140, 498, 721, 1137, 1510, 1630
CviQI GTAC 7 cut(s) 112, 140, 498, 721, 1137, 1510, 1630
DdeI CTNAG 5 cut(s) 101, 602, 933, 1436, 1455
DpnI GATC 5 cut(s) 826, 990, 1020, 1340, 1375
DpnII GATC 5 cut(s) 824, 988, 1018, 1338, 1373
Eam1104I CTCTTC 1 cut(s) 180
EarI CTCTTC 1 cut(s) 180
EciI GGCGGA 1 cut(s) 274
Ecl136II GAGCTC 1 cut(s) 717
Eco130I CCWWGG 4 cut(s) 240, 376, 389, 790
Eco24I GRGCYC 1 cut(s) 719
Eco31I GGTCTC 1 cut(s) 578
Eco47I GGWCC 3 cut(s) 268, 385, 401
Eco53kI GAGCTC 1 cut(s) 717
Eco81I CCTNAGG 1 cut(s) 933
Eco91I GGTNACC 1 cut(s) 955
EcoICRI GAGCTC 1 cut(s) 717
EcoNI CCTNNNNNAGG 1 cut(s) 380
EcoO109I RGGNCCY 1 cut(s) 385
EcoO65I GGTNACC 1 cut(s) 955
EcoRI GAATTC 1 cut(s) 1803
EcoRII CCWGG 2 cut(s) 1108, 1735
EcoT14I CCWWGG 4 cut(s) 240, 376, 389, 790
EcoT38I GRGCYC 1 cut(s) 719
ErhI CCWWGG 4 cut(s) 240, 376, 389, 790
FalI AAGNNNNNCTT 2 cut(s) 336, 368
FaqI GGGAC 4 cut(s) 358, 444, 480, 674
FauI CCCGC 1 cut(s) 224
FauNDI CATATG 1 cut(s) 626
FblI GTMKAC 1 cut(s) 1587
Fnu4HI GCNGC 8 cut(s) 639, 966, 1024, 1027, 1232, 1526, 1608, 1611
FokI GGATG 5 cut(s) 73, 194, 750, 860, 1453
FriOI GRGCYC 1 cut(s) 719
Fsp4HI GCNGC 8 cut(s) 639, 966, 1024, 1027, 1232, 1526, 1608, 1611
FspBI CTAG 5 cut(s) 156, 434, 974, 1461, 1769
GlaI GCGC 3 cut(s) 1134, 1149, 1734
GluI GCNGC 8 cut(s) 639, 966, 1024, 1027, 1232, 1526, 1608, 1611
GsuI CTGGAG 1 cut(s) 194
HaeII RGCGCY 1 cut(s) 1151
HaeIII GGCC 3 cut(s) 293, 485, 1490
HapII CCGG 3 cut(s) 74, 281, 482
HgaI GACGC 3 cut(s) 533, 1590, 1628
HhaI GCGC 3 cut(s) 1135, 1150, 1735
Hin6I GCGC 3 cut(s) 1133, 1148, 1733
HinP1I GCGC 3 cut(s) 1133, 1148, 1733
HincII GTYRAC 2 cut(s) 675, 1618
HindII GTYRAC 2 cut(s) 675, 1618
HindIII AAGCTT 1 cut(s) 1364
HinfI GANTC 8 cut(s) 123, 198, 795, 1158, 1168, 1424, 1547, 1589
HpaII CCGG 3 cut(s) 74, 281, 482
HphI GGTGA 2 cut(s) 323, 577
Hpy166II GTNNAC 7 cut(s) 268, 675, 760, 1588, 1618, 1678, 1717
Hpy188I TCNGA 9 cut(s) 203, 223, 737, 915, 1378, 1423, 1456, 1505, 1648
Hpy8I GTNNAC 7 cut(s) 268, 675, 760, 1588, 1618, 1678, 1717
Hpy99I CGWCG 2 cut(s) 329, 527
HpyAV CCTTC 6 cut(s) 198, 239, 247, 1238, 1376, 1526
HpyCH4III ACNGT 2 cut(s) 1286, 1419
HpyCH4IV ACGT 3 cut(s) 142, 1499, 1512
HpyF10VI GCNNNNNNNGC 6 cut(s) 290, 1363, 1487, 1607, 1619, 1684
HpyF3I CTNAG 5 cut(s) 101, 602, 933, 1436, 1455
HpySE526I ACGT 3 cut(s) 142, 1499, 1512
HspAI GCGC 3 cut(s) 1133, 1148, 1733
KroI GCCGGC 1 cut(s) 73
KroNI GCCGGC 1 cut(s) 75
Kzo9I GATC 5 cut(s) 824, 988, 1018, 1338, 1373
LmnI GCTCC 3 cut(s) 213, 340, 1319
Lsp1109I GCAGC 6 cut(s) 650, 952, 1035, 1243, 1594, 1597
LweI GCATC 3 cut(s) 554, 1120, 1588
MaeI CTAG 5 cut(s) 156, 434, 974, 1461, 1769
MaeII ACGT 3 cut(s) 142, 1499, 1512
MaeIII GTNAC 2 cut(s) 955, 1123
MalI GATC 5 cut(s) 826, 990, 1020, 1340, 1375
MboI GATC 5 cut(s) 824, 988, 1018, 1338, 1373
MboII GAAGA 6 cut(s) 12, 144, 197, 632, 1424, 1757
MhlI GDGCHC 3 cut(s) 719, 1291, 1680
MluCI AATT 9 cut(s) 27, 134, 406, 501, 578, 1449, 1775, 1803, 1845
MlyI GAGTC 2 cut(s) 1433, 1583
MmeI TCCRAC 2 cut(s) 834, 1369
MroNI GCCGGC 1 cut(s) 73
MroXI GAANNNNTTC 1 cut(s) 1304
MseI TTAA 4 cut(s) 894, 1236, 1293, 1815
MslI CAYNNNNRTG 3 cut(s) 1013, 1121, 1349
MspA1I CMGCKG 2 cut(s) 1026, 1607
MspI CCGG 3 cut(s) 74, 281, 482
MspR9I CCNGG 2 cut(s) 1110, 1737
Mva1269I GAATGC 2 cut(s) 817, 1692
MvaI CCWGG 2 cut(s) 1110, 1737
MvnI CGCG 2 cut(s) 231, 1135
MwoI GCNNNNNNNGC 6 cut(s) 290, 1363, 1487, 1607, 1619, 1684
NaeI GCCGGC 1 cut(s) 75
NdeI CATATG 1 cut(s) 626
NdeII GATC 5 cut(s) 824, 988, 1018, 1338, 1373
NgoMIV GCCGGC 1 cut(s) 73
NlaIV GGNNCC 3 cut(s) 209, 387, 1114
NmuCI GTSAC 1 cut(s) 1123
NspI RCATGY 2 cut(s) 1630, 1723
OliI CACNNNNGTG 2 cut(s) 1013, 1121
PagI TCATGA 4 cut(s) 492, 534, 711, 1263
PciI ACATGT 1 cut(s) 1626
PctI GAATGC 2 cut(s) 817, 1692
PdiI GCCGGC 1 cut(s) 75
PdmI GAANNNNTTC 1 cut(s) 1304
PfeI GAWTC 6 cut(s) 123, 198, 795, 1158, 1168, 1547
Pfl23II CGTACG 1 cut(s) 720
PflFI GACNNNGTC 1 cut(s) 435
PkrI GCNGC 8 cut(s) 640, 967, 1025, 1028, 1233, 1527, 1609, 1612
PleI GAGTC 2 cut(s) 1432, 1583
PpsI GAGTC 2 cut(s) 1432, 1583
Ppu21I YACGTR 1 cut(s) 143
PpuMI RGGWCCY 1 cut(s) 385
PscI ACATGT 1 cut(s) 1626
Psp124BI GAGCTC 1 cut(s) 719
Psp1406I AACGTT 1 cut(s) 1499
Psp5II RGGWCCY 1 cut(s) 385
Psp6I CCWGG 2 cut(s) 1108, 1735
PspEI GGTNACC 1 cut(s) 955
PspGI CCWGG 2 cut(s) 1108, 1735
PspLI CGTACG 1 cut(s) 720
PspN4I GGNNCC 3 cut(s) 209, 387, 1114
PspPI GGNCC 3 cut(s) 268, 385, 401
PspPPI RGGWCCY 1 cut(s) 385
PstI CTGCAG 1 cut(s) 970
PstNI CAGNNNCTG 1 cut(s) 1610
PsyI GACNNNGTC 1 cut(s) 435
PvuII CAGCTG 1 cut(s) 1607
RsaI GTAC 7 cut(s) 113, 141, 499, 722, 1138, 1511, 1631
RsaNI GTAC 7 cut(s) 112, 140, 498, 721, 1137, 1510, 1630
RseI CAYNNNNRTG 3 cut(s) 1013, 1121, 1349
SacI GAGCTC 1 cut(s) 719
SaqAI TTAA 4 cut(s) 894, 1236, 1293, 1815
SatI GCNGC 8 cut(s) 639, 966, 1024, 1027, 1232, 1526, 1608, 1611
Sau3AI GATC 5 cut(s) 824, 988, 1018, 1338, 1373
Sau96I GGNCC 3 cut(s) 268, 385, 401
SchI GAGTC 2 cut(s) 1433, 1583
ScrFI CCNGG 2 cut(s) 1110, 1737
SduI GDGCHC 3 cut(s) 719, 1291, 1680
SfaNI GCATC 3 cut(s) 554, 1120, 1588
SfcI CTRYAG 1 cut(s) 966
SinI GGWCC 3 cut(s) 268, 385, 401
SmiMI CAYNNNNRTG 3 cut(s) 1013, 1121, 1349
Sse9I AATT 9 cut(s) 27, 134, 406, 501, 578, 1449, 1775, 1803, 1845
SspI AATATT 1 cut(s) 1708
SspMI CTAG 5 cut(s) 156, 434, 974, 1461, 1769
SstI GAGCTC 1 cut(s) 719
StyD4I CCNGG 2 cut(s) 1108, 1735
StyI CCWWGG 4 cut(s) 240, 376, 389, 790
TaaI ACNGT 2 cut(s) 1286, 1419
TaiI ACGT 3 cut(s) 145, 1502, 1515
TaqI TCGA 5 cut(s) 324, 459, 618, 1207, 1347
TaqII GACCGA 1 cut(s) 1142
TasI AATT 9 cut(s) 27, 134, 406, 501, 578, 1449, 1775, 1803, 1845
TatI WGTACW 2 cut(s) 497, 1629
TauI GCSGC 2 cut(s) 1029, 1528
TfiI GAWTC 6 cut(s) 123, 198, 795, 1158, 1168, 1547
Tru1I TTAA 4 cut(s) 894, 1236, 1293, 1815
Tru9I TTAA 4 cut(s) 894, 1236, 1293, 1815
TscAI CASTG 5 cut(s) 166, 975, 1015, 1488, 1719
TseFI GTSAC 1 cut(s) 1123
TseI GCWGC 6 cut(s) 638, 965, 1023, 1231, 1607, 1610
Tsp45I GTSAC 1 cut(s) 1123
TspDTI ATGAA 8 cut(s) 186, 198, 481, 700, 1150, 1257, 1376, 1457
TspGWI ACGGA 3 cut(s) 99, 756, 918
TspRI CASTG 5 cut(s) 166, 975, 1015, 1488, 1719
Tth111I GACNNNGTC 1 cut(s) 435
VneI GTGCAC 1 cut(s) 1676
VpaK11BI GGWCC 3 cut(s) 268, 385, 401
XagI CCTNNNNNAGG 1 cut(s) 380
XapI RAATTY 5 cut(s) 134, 406, 578, 1775, 1803
XbaI TCTAGA 1 cut(s) 1768
XceI RCATGY 2 cut(s) 1630, 1723
XmiI GTMKAC 1 cut(s) 1587
XmnI GAANNNNTTC 1 cut(s) 1304
XspI CTAG 5 cut(s) 156, 434, 974, 1461, 1769
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.