pycom16g02240

Phytanoyl-CoA dioxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0014313
Physical Location & Seq
Reverse (-)
1439568 .. 1441977
2410 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 795 bp
ATGGAATCGTTTGCGAGCGCGGAGGACATCGAGGCCATGAGGAGCAGAATGGACCAATTGCTCCAAGACTTTGACTGCTCTTCCACCGCCTCTATTTTCTCCACCAAGAACCAGCAACAGACTACTGATAATTACTTCTATGATAGCGTCGACAACATTTCGTTTTTCTTCGAAGAGAAAGCCTTTGGGAGTGATGGAGAACTGCAACAGCCGAAGGAACTTTCGATTAACAAAGTTGGCCATGCGTTACATGAGCTCGATCCAGTATTTAAAGGGATCACGTATTCTGAGAAAATTTCAGGCTTGTTCTCCTCCTTGGGTTACAAAAGGCCCATTGTCATTCAGTCTATGTACATTTTCAAGCAACCAGGAATCGGAGGTGAGGTAGTGCCACATCAGGATAATTCATTTCTTTATACCGATCCACCAACTTGCACAGGGTTGTGGCTGGCTTTAGAAGATGCAACAATAACGAATGGCTGCCTATGGGCTATTCCTGGATCTCAAAAGAATGGGCTTGTACGACGGTTCATTAGAGGAGAAGACGGTGTGACCTTTGATCGGCCTTCCCCTGTGTATGATCAAAAAGATTTTATTCCCATCGAAGTGAAAGCTGGGTCTTTAGTTGTTATTCACGGTGATCTTATTCATCAAAGCTTCGAGAATCAGTCCTCGAAATCAAGGCATGCGTACAGCTTGCATGTGGTGGATACCGATGGCTGCAAATGGTCAAAAGATAATTGGATCAGGAGAAAAGTGGAGCCAGAGCCTCTCTATGTATCTTCTTTGACCTAA

Protein Analysis

265

Amino Acids

29.75

Weight (kDa)

5.08

Isoelectric Point (pI)

54.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PhyH PF05721 2 - 230 5e-50 Phytanoyl-CoA dioxygenase (PhyH)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014313)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G01490 AT2G01490
fragaria_vesca FvH4_4g32640
malus_domestica MD13G1023900.v1.1 MD16G1026600.v1.1
prunus_persica Prupe.1G326800_v2.0.a1
pyrus_communis pycom16g02240
rosa_chinensis RchiOBHm_Chr4g0441521
rosa_laevigata RLG00000006088
rosa_roxburghii Rroxscaffold_5G00382370 Rroxscaffold_7G00185860
rosa_rugosa Rorug04G0334400 Rorug06G0150600
rosa_samantha Rh4BG399900 Rh4CG414900
rosa_wichuraiana Rw4G033320 Rw6G022810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 150
AccII CGCG 1 cut(s) 20
AciI CCGC 2 cut(s) 20, 87
AclWI GGATC 5 cut(s) 254, 284, 416, 508, 752
AcoI YGGCCR 1 cut(s) 238
AcsI RAATTY 1 cut(s) 294
AfaI GTAC 3 cut(s) 353, 522, 692
AfiI CCNNNNNNNGG 2 cut(s) 374, 561
AgsI TTSAA 1 cut(s) 361
AjnI CCWGG 2 cut(s) 367, 496
AluBI AGCT 4 cut(s) 256, 614, 657, 696
AluI AGCT 4 cut(s) 256, 614, 657, 696
Alw21I GWGCWC 1 cut(s) 258
AlwI GGATC 5 cut(s) 254, 284, 416, 508, 752
AoxI GGCC 4 cut(s) 33, 238, 329, 563
ApeKI GCWGC 2 cut(s) 480, 720
ApoI RAATTY 1 cut(s) 294
AspLEI GCGC 1 cut(s) 20
AspS9I GGNCC 2 cut(s) 52, 330
AsuHPI GGTGA 2 cut(s) 392, 650
AsuII TTCGAA 1 cut(s) 171
AvaII GGWCC 1 cut(s) 52
BalI TGGCCA 1 cut(s) 240
BanII GRGCYC 1 cut(s) 258
BbsI GAAGAC 1 cut(s) 549
Bbv12I GWGCWC 1 cut(s) 258
BbvI GCAGC 2 cut(s) 467, 707
BccI CCATC 3 cut(s) 188, 608, 710
BciT130I CCWGG 2 cut(s) 369, 498
BciVI GTATCC 1 cut(s) 703
BclI TGATCA 1 cut(s) 580
BfuI GTATCC 1 cut(s) 703
BisI GCNGC 2 cut(s) 481, 721
BlsI GCNGC 2 cut(s) 482, 722
Bme1390I CCNGG 2 cut(s) 369, 498
Bme18I GGWCC 1 cut(s) 52
BmgT120I GGNCC 2 cut(s) 52, 330
BmiI GGNNCC 1 cut(s) 762
BmrFI CCNGG 2 cut(s) 369, 498
BmsI GCATC 1 cut(s) 451
BpiI GAAGAC 1 cut(s) 549
Bpu14I TTCGAA 1 cut(s) 171
BsaAI YACGTR 1 cut(s) 282
BsaJI CCNNGG 1 cut(s) 315
BsaXI ACNNNNNCTCC 2 cut(s) 369, 399
Bsc4I CCNNNNNNNGG 2 cut(s) 374, 561
Bse1I ACTGG 1 cut(s) 263
BseBI CCWGG 2 cut(s) 369, 498
BseDI CCNNGG 1 cut(s) 315
BseLI CCNNNNNNNGG 2 cut(s) 374, 561
BseMII CTCAG 1 cut(s) 279
BseNI ACTGG 1 cut(s) 263
BseRI GAGGAG 3 cut(s) 55, 301, 552
BseXI GCAGC 2 cut(s) 467, 707
BseYI CCCAGC 1 cut(s) 614
Bsh1236I CGCG 1 cut(s) 20
BshFI GGCC 4 cut(s) 35, 240, 331, 565
BsiHKAI GWGCWC 1 cut(s) 258
BslI CCNNNNNNNGG 2 cut(s) 374, 561
BsnI GGCC 4 cut(s) 35, 240, 331, 565
Bsp119I TTCGAA 1 cut(s) 171
Bsp1286I GDGCHC 1 cut(s) 258
Bsp1407I TGTACA 1 cut(s) 351
Bsp143I GATC 8 cut(s) 259, 276, 421, 500, 559, 580, 640, 744
BspACI CCGC 2 cut(s) 20, 87
BspANI GGCC 4 cut(s) 35, 240, 331, 565
BspCNI CTCAG 1 cut(s) 280
BspFNI CGCG 1 cut(s) 20
BspLI GGNNCC 1 cut(s) 762
BspPI GGATC 5 cut(s) 254, 284, 416, 508, 752
BspQI GCTCTTC 1 cut(s) 85
BspT104I TTCGAA 1 cut(s) 171
BsrGI TGTACA 1 cut(s) 351
BsrI ACTGG 1 cut(s) 263
BssECI CCNNGG 1 cut(s) 315
BssMI GATC 8 cut(s) 259, 276, 421, 500, 559, 580, 640, 744
BssT1I CCWWGG 1 cut(s) 315
Bst2UI CCWGG 2 cut(s) 369, 498
Bst4CI ACNGT 3 cut(s) 528, 548, 638
Bst6I CTCTTC 2 cut(s) 85, 168
BstAUI TGTACA 1 cut(s) 351
BstBAI YACGTR 1 cut(s) 282
BstBI TTCGAA 1 cut(s) 171
BstC8I GCNNGC 4 cut(s) 16, 450, 687, 698
BstDEI CTNAG 1 cut(s) 288
BstFNI CGCG 1 cut(s) 20
BstHHI GCGC 1 cut(s) 20
BstKTI GATC 8 cut(s) 262, 279, 424, 503, 562, 583, 643, 747
BstMBI GATC 8 cut(s) 259, 276, 421, 500, 559, 580, 640, 744
BstNI CCWGG 2 cut(s) 369, 498
BstNSI RCATGY 2 cut(s) 689, 704
BstSCI CCNGG 2 cut(s) 367, 496
BstUI CGCG 1 cut(s) 20
BstV1I GCAGC 2 cut(s) 467, 707
BstV2I GAAGAC 1 cut(s) 549
BstX2I RGATCY 1 cut(s) 500
BstYI RGATCY 1 cut(s) 500
BsuI GTATCC 1 cut(s) 703
BsuRI GGCC 4 cut(s) 35, 240, 331, 565
Cac8I GCNNGC 4 cut(s) 16, 450, 687, 698
CfoI GCGC 1 cut(s) 20
Cfr13I GGNCC 2 cut(s) 52, 330
CseI GACGC 1 cut(s) 136
Csp6I GTAC 3 cut(s) 352, 521, 691
CviAII CATG 5 cut(s) 37, 242, 251, 686, 701
CviQI GTAC 3 cut(s) 352, 521, 691
DdeI CTNAG 1 cut(s) 288
DpnI GATC 8 cut(s) 261, 278, 423, 502, 561, 582, 642, 746
DpnII GATC 8 cut(s) 259, 276, 421, 500, 559, 580, 640, 744
DraI TTTAAA 1 cut(s) 271
EaeI YGGCCR 1 cut(s) 238
Eam1104I CTCTTC 2 cut(s) 85, 168
EarI CTCTTC 2 cut(s) 85, 168
Ecl136II GAGCTC 1 cut(s) 256
Eco130I CCWWGG 1 cut(s) 315
Eco24I GRGCYC 1 cut(s) 258
Eco47I GGWCC 1 cut(s) 52
Eco53kI GAGCTC 1 cut(s) 256
EcoICRI GAGCTC 1 cut(s) 256
EcoRII CCWGG 2 cut(s) 367, 496
EcoT14I CCWWGG 1 cut(s) 315
EcoT38I GRGCYC 1 cut(s) 258
ErhI CCWWGG 1 cut(s) 315
FaeI CATG 5 cut(s) 40, 245, 254, 689, 704
FatI CATG 5 cut(s) 36, 241, 250, 685, 700
FbaI TGATCA 1 cut(s) 580
FblI GTMKAC 1 cut(s) 150
Fnu4HI GCNGC 2 cut(s) 481, 721
FriOI GRGCYC 1 cut(s) 258
Fsp4HI GCNGC 2 cut(s) 481, 721
GlaI GCGC 1 cut(s) 19
GluI GCNGC 2 cut(s) 481, 721
GsaI CCCAGC 1 cut(s) 618
HaeIII GGCC 4 cut(s) 35, 240, 331, 565
HgaI GACGC 1 cut(s) 136
HhaI GCGC 1 cut(s) 20
Hin1II CATG 5 cut(s) 40, 245, 254, 689, 704
Hin6I GCGC 1 cut(s) 18
HinP1I GCGC 1 cut(s) 18
HincII GTYRAC 1 cut(s) 151
HindII GTYRAC 1 cut(s) 151
HindIII AAGCTT 1 cut(s) 655
HinfI GANTC 3 cut(s) 5, 372, 664
HphI GGTGA 2 cut(s) 392, 650
Hpy166II GTNNAC 1 cut(s) 151
Hpy188I TCNGA 2 cut(s) 289, 377
Hpy188III TCNNGA 3 cut(s) 398, 661, 748
Hpy8I GTNNAC 1 cut(s) 151
Hpy99I CGWCG 2 cut(s) 152, 528
HpyAV CCTTC 2 cut(s) 208, 576
HpyCH4III ACNGT 3 cut(s) 528, 548, 638
HpyCH4IV ACGT 1 cut(s) 281
HpyCH4V TGCA 5 cut(s) 205, 435, 464, 700, 723
HpyF3I CTNAG 1 cut(s) 288
HpySE526I ACGT 1 cut(s) 281
Hsp92II CATG 5 cut(s) 40, 245, 254, 689, 704
HspAI GCGC 1 cut(s) 18
Ksp22I TGATCA 1 cut(s) 580
Kzo9I GATC 8 cut(s) 259, 276, 421, 500, 559, 580, 640, 744
LguI GCTCTTC 1 cut(s) 85
LmnI GCTCC 3 cut(s) 42, 66, 760
Lsp1109I GCAGC 2 cut(s) 467, 707
LweI GCATC 1 cut(s) 451
MaeII ACGT 1 cut(s) 281
MaeIII GTNAC 3 cut(s) 246, 320, 550
MalI GATC 8 cut(s) 261, 278, 423, 502, 561, 582, 642, 746
MboI GATC 8 cut(s) 259, 276, 421, 500, 559, 580, 640, 744
MboII GAAGA 6 cut(s) 72, 160, 185, 470, 554, 774
MfeI CAATTG 1 cut(s) 56
MflI RGATCY 1 cut(s) 500
MhlI GDGCHC 1 cut(s) 258
MlsI TGGCCA 1 cut(s) 240
MluCI AATT 5 cut(s) 56, 130, 294, 403, 739
MluNI TGGCCA 1 cut(s) 240
Mox20I TGGCCA 1 cut(s) 240
MscI TGGCCA 1 cut(s) 240
MseI TTAA 2 cut(s) 228, 270
MslI CAYNNNNRTG 1 cut(s) 605
Msp20I TGGCCA 1 cut(s) 240
MspR9I CCNGG 2 cut(s) 369, 498
MunI CAATTG 1 cut(s) 56
MvaI CCWGG 2 cut(s) 369, 498
MvnI CGCG 1 cut(s) 20
NdeII GATC 8 cut(s) 259, 276, 421, 500, 559, 580, 640, 744
NlaIII CATG 5 cut(s) 40, 245, 254, 689, 704
NlaIV GGNNCC 1 cut(s) 762
NmuCI GTSAC 1 cut(s) 550
NspI RCATGY 2 cut(s) 689, 704
NspV TTCGAA 1 cut(s) 171
PaeI GCATGC 1 cut(s) 689
PciSI GCTCTTC 1 cut(s) 85
PfeI GAWTC 3 cut(s) 5, 372, 664
PfoI TCCNGGA 1 cut(s) 496
PkrI GCNGC 2 cut(s) 482, 722
Ppu21I YACGTR 1 cut(s) 282
Psp124BI GAGCTC 1 cut(s) 258
Psp6I CCWGG 2 cut(s) 367, 496
PspFI CCCAGC 1 cut(s) 614
PspGI CCWGG 2 cut(s) 367, 496
PspN4I GGNNCC 1 cut(s) 762
PspPI GGNCC 2 cut(s) 52, 330
PsuI RGATCY 1 cut(s) 500
RsaI GTAC 3 cut(s) 353, 522, 692
RsaNI GTAC 3 cut(s) 352, 521, 691
RseI CAYNNNNRTG 1 cut(s) 605
SacI GAGCTC 1 cut(s) 258
SalI GTCGAC 1 cut(s) 149
SapI GCTCTTC 1 cut(s) 85
SaqAI TTAA 2 cut(s) 228, 270
SatI GCNGC 2 cut(s) 481, 721
Sau3AI GATC 8 cut(s) 259, 276, 421, 500, 559, 580, 640, 744
Sau96I GGNCC 2 cut(s) 52, 330
ScrFI CCNGG 2 cut(s) 369, 498
SduI GDGCHC 1 cut(s) 258
SetI ASST 9 cut(s) 258, 284, 382, 387, 557, 616, 659, 698, 794
SfaNI GCATC 1 cut(s) 451
SfuI TTCGAA 1 cut(s) 171
SinI GGWCC 1 cut(s) 52
SmiMI CAYNNNNRTG 1 cut(s) 605
SphI GCATGC 1 cut(s) 689
Sse9I AATT 5 cut(s) 56, 130, 294, 403, 739
SsiI CCGC 2 cut(s) 20, 87
SstI GAGCTC 1 cut(s) 258
StyD4I CCNGG 2 cut(s) 367, 496
StyI CCWWGG 1 cut(s) 315
TaaI ACNGT 3 cut(s) 528, 548, 638
TaiI ACGT 1 cut(s) 284
TaqI TCGA 8 cut(s) 30, 150, 171, 224, 258, 603, 660, 674
TasI AATT 5 cut(s) 56, 130, 294, 403, 739
TatI WGTACW 1 cut(s) 351
TfiI GAWTC 3 cut(s) 5, 372, 664
Tru1I TTAA 2 cut(s) 228, 270
Tru9I TTAA 2 cut(s) 228, 270
TseFI GTSAC 1 cut(s) 550
TseI GCWGC 2 cut(s) 480, 720
Tsp45I GTSAC 1 cut(s) 550
TspDTI ATGAA 3 cut(s) 396, 520, 638
VpaK11BI GGWCC 1 cut(s) 52
XapI RAATTY 1 cut(s) 294
XceI RCATGY 2 cut(s) 689, 704
XmiI GTMKAC 1 cut(s) 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.