pycom16g05940

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0011117
Physical Location & Seq
Forward (+)
3784939 .. 3786666
1728 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1008 bp
ATGTTTAGTAGAAAATATGGATATAAACCGCTCCAGATGCCTCCAATTAGTTGGTCAAACGAAAAATACTACCTGGAACAGATTTTGCCACTTATTAGCAAGCATAAGGTGGTGCACTTTAACAGAACAGATGCACGCTTGGCAAACAATCGGATACCTCCTGATCTTCAGAAACTCCGGTGTCGTGTTAATTTCCAGGCACTGAAGTTCACTCCCGAGATCGAGGCTTTAGGGTACAAATTGGTTCGTATACTTCAAGAAAAGGGACCTTATGTGGCTTTGCATCTAAGGTACGAGATGGACATGTTGGCTTTCTCAGGTTGTACTCATGGCTGCACTGAGGAAGAAGCTGACGAGCTCAAACGATTGAGATACGCATATCCTTGGTGGAGAGAGAAAGAGATAGTGTCTGAAGAGAGGAGATCTCAAGGTCTTTGTCCTCTAACACCCGAGGAGAGTGCATTAATTTTGCAAGCTTTGGGTTTTAGTACGGATACACAAATCTATATAGCAGCTGGTGAGATTTATGGTAGTGAAAGGAGACTGGGTCCGCTACGGGCTGCATTTCCACAGATTGTTAAGAAGGAAACACTTCTTGCTCCGGAGGAGTTGCAACAGTTTCAGAATCATTCATCTCAAATGGCAGCTTTGGATTTTATAGTTTCGGTAGCTAGTAATACTTTTGTTCCTACATATGATGGGAACATGGCGAAACTTGTTGAAGGTCACCGAAGATACCTTGGGTTTAAAAAAAGCATATTGCTGGATAGGAAAAGACTTGTGGAGTTGCTGGACTTGCATCTTAATGGAACACATACATGGAATGAATTTTCAGATGCCGTGCGATCCGTACACGAGAAAAGAATGGGACAGCCAACTCGACGCAGAGTTATTGCAGACAGGCCAAAGGAGGAAGACTATTTTTATGCAAATCCGCAGGAGTGCCTCTGCGAGGAAACAAATTGCGACGGCTTGCTTAGCCTTGGCAACTCAAGTAAAACTGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

336

Amino Acids

38.7

Weight (kDa)

8.36

Isoelectric Point (pI)

59.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 15 - 247 1.4e-75 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011117)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14020 AT2G03280
fragaria_vesca FvH4_4g30810
malus_domestica MD00G1080900.v1.1 MD16G1067800.v1.1
prunus_persica Prupe.1G279000_v2.0.a1
pyrus_communis pycom13g06080 pycom16g05930 pycom16g05940
rosa_chinensis RchiOBHm_Chr4g0439201
rosa_laevigata RLG00000006288
rosa_multiflora Rmu_sc0002236.1_g000012
rosa_roxburghii Rroxscaffold_5G00380190
rosa_rugosa Rorug04G0317000
rosa_samantha Rh4AG367500 Rh4CG393500 Rh4DG373700
rosa_wichuraiana Rw0G005660 Rw0G011650 Rw5G028530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 31
AccI GTMKAC 1 cut(s) 250
AccIII TCCGGA 1 cut(s) 601
AciI CCGC 3 cut(s) 29, 551, 935
AclWI GGATC 1 cut(s) 840
AcsI RAATTY 1 cut(s) 827
AcuI CTGAAG 3 cut(s) 152, 224, 432
AfaI GTAC 5 cut(s) 236, 293, 325, 490, 852
AfiI CCNNNNNNNGG 1 cut(s) 952
AflIII ACRYGT 1 cut(s) 303
AgsI TTSAA 2 cut(s) 257, 722
AjnI CCWGG 2 cut(s) 72, 195
AluBI AGCT 6 cut(s) 350, 358, 476, 515, 647, 671
AluI AGCT 6 cut(s) 350, 358, 476, 515, 647, 671
Alw21I GWGCWC 2 cut(s) 117, 360
Alw26I GTCTC 1 cut(s) 535
Alw44I GTGCAC 1 cut(s) 113
AlwI GGATC 1 cut(s) 840
AlwNI CAGNNNCTG 1 cut(s) 202
Ama87I CYCGRG 2 cut(s) 215, 449
Aor13HI TCCGGA 1 cut(s) 601
AoxI GGCC 1 cut(s) 902
ApaLI GTGCAC 1 cut(s) 113
ApeKI GCWGC 4 cut(s) 333, 512, 560, 644
ApoI RAATTY 1 cut(s) 827
AseI ATTAAT 1 cut(s) 464
Asp700I GAANNNNTTC 1 cut(s) 591
AspS9I GGNCC 2 cut(s) 266, 548
AsuHPI GGTGA 2 cut(s) 530, 719
AvaI CYCGRG 2 cut(s) 215, 449
AvaII GGWCC 2 cut(s) 266, 548
BaeGI GKGCMC 1 cut(s) 117
BanII GRGCYC 1 cut(s) 360
BauI CACGAG 1 cut(s) 854
BbsI GAAGAC 1 cut(s) 921
Bbv12I GWGCWC 2 cut(s) 117, 360
BbvI GCAGC 4 cut(s) 320, 524, 547, 656
BccI CCATC 2 cut(s) 292, 692
BceAI ACGGC 2 cut(s) 824, 985
BcgI CGANNNNNNTGC 2 cut(s) 440, 474
BciT130I CCWGG 2 cut(s) 74, 197
BciVI GTATCC 2 cut(s) 147, 487
BcoDI GTCTC 1 cut(s) 535
BfaI CTAG 1 cut(s) 672
BfuI GTATCC 2 cut(s) 147, 487
BglII AGATCT 1 cut(s) 422
BisI GCNGC 4 cut(s) 334, 513, 561, 645
BlpI GCTNAGC 1 cut(s) 977
BlsI GCNGC 4 cut(s) 335, 514, 562, 646
Bme1390I CCNGG 2 cut(s) 74, 197
Bme18I GGWCC 2 cut(s) 266, 548
BmeT110I CYCGRG 2 cut(s) 215, 449
BmgT120I GGNCC 2 cut(s) 266, 548
BmiI GGNNCC 2 cut(s) 267, 549
BmrFI CCNGG 2 cut(s) 74, 197
BmrI ACTGGG 1 cut(s) 554
BmsI GCATC 5 cut(s) 27, 121, 292, 808, 826
BmuI ACTGGG 1 cut(s) 554
BpiI GAAGAC 1 cut(s) 921
BplI GAGNNNNNCTC 2 cut(s) 409, 441
BpmI CTGGAG 1 cut(s) 17
Bpu1102I GCTNAGC 1 cut(s) 977
BpuEI CTTGAG 2 cut(s) 411, 976
BsaJI CCNNGG 4 cut(s) 383, 450, 739, 982
BsaWI WCCGGW 2 cut(s) 177, 601
BsaXI ACNNNNNCTCC 2 cut(s) 532, 562
Bsc4I CCNNNNNNNGG 1 cut(s) 952
Bse1I ACTGG 2 cut(s) 549, 1006
BseAI TCCGGA 1 cut(s) 601
BseBI CCWGG 2 cut(s) 74, 197
BseDI CCNNGG 4 cut(s) 383, 450, 739, 982
BseLI CCNNNNNNNGG 1 cut(s) 952
BseMII CTCAG 2 cut(s) 330, 330
BseNI ACTGG 2 cut(s) 549, 1006
BseRI GAGGAG 3 cut(s) 433, 467, 620
BseSI GKGCMC 1 cut(s) 117
BseXI GCAGC 4 cut(s) 320, 524, 547, 656
BsgI GTGCAG 1 cut(s) 319
BshFI GGCC 1 cut(s) 904
BsiHKAI GWGCWC 2 cut(s) 117, 360
BsiHKCI CYCGRG 2 cut(s) 215, 449
BsiSI CCGG 2 cut(s) 178, 602
BslFI GGGAC 2 cut(s) 279, 882
BslI CCNNNNNNNGG 1 cut(s) 952
BsmAI GTCTC 1 cut(s) 535
BsmFI GGGAC 2 cut(s) 279, 882
BsnI GGCC 1 cut(s) 904
BsoBI CYCGRG 2 cut(s) 215, 449
Bsp1286I GDGCHC 2 cut(s) 117, 360
Bsp13I TCCGGA 1 cut(s) 601
Bsp143I GATC 4 cut(s) 163, 219, 422, 845
Bsp1720I GCTNAGC 1 cut(s) 977
BspACI CCGC 3 cut(s) 29, 551, 935
BspANI GGCC 1 cut(s) 904
BspCNI CTCAG 2 cut(s) 329, 331
BspEI TCCGGA 1 cut(s) 601
BspLI GGNNCC 2 cut(s) 267, 549
BspPI GGATC 1 cut(s) 840
BsrBI CCGCTC 1 cut(s) 31
BsrI ACTGG 2 cut(s) 549, 1006
BssECI CCNNGG 4 cut(s) 383, 450, 739, 982
BssMI GATC 4 cut(s) 163, 219, 422, 845
BssNAI GTATAC 1 cut(s) 251
BssSI CACGAG 1 cut(s) 854
BssT1I CCWWGG 3 cut(s) 383, 739, 982
Bst1107I GTATAC 1 cut(s) 251
Bst2BI CACGAG 1 cut(s) 854
Bst2UI CCWGG 2 cut(s) 74, 197
Bst4CI ACNGT 1 cut(s) 618
Bst6I CTCTTC 1 cut(s) 408
BstC8I GCNNGC 4 cut(s) 101, 136, 474, 974
BstDEI CTNAG 4 cut(s) 287, 316, 339, 977
BstEII GGTNACC 1 cut(s) 725
BstENI CCTNNNNNAGG 1 cut(s) 950
BstKTI GATC 4 cut(s) 166, 222, 425, 848
BstMAI GTCTC 1 cut(s) 535
BstMBI GATC 4 cut(s) 163, 219, 422, 845
BstMWI GCNNNNNNNGC 4 cut(s) 37, 140, 796, 978
BstNI CCWGG 2 cut(s) 74, 197
BstNSI RCATGY 1 cut(s) 307
BstPI GGTNACC 1 cut(s) 725
BstSCI CCNGG 2 cut(s) 72, 195
BstSLI GKGCMC 1 cut(s) 117
BstV1I GCAGC 4 cut(s) 320, 524, 547, 656
BstV2I GAAGAC 1 cut(s) 921
BstX2I RGATCY 1 cut(s) 422
BstXI CCANNNNNNTGG 1 cut(s) 51
BstYI RGATCY 1 cut(s) 422
BstZ17I GTATAC 1 cut(s) 251
BsuI GTATCC 2 cut(s) 147, 487
BsuRI GGCC 1 cut(s) 904
BtsIMutI CAGTG 2 cut(s) 200, 336
Cac8I GCNNGC 4 cut(s) 101, 136, 474, 974
CaiI CAGNNNCTG 1 cut(s) 202
Cfr13I GGNCC 2 cut(s) 266, 548
CseI GACGC 1 cut(s) 891
Csp6I GTAC 5 cut(s) 235, 292, 324, 489, 851
CviAII CATG 4 cut(s) 304, 329, 706, 819
CviQI GTAC 5 cut(s) 235, 292, 324, 489, 851
DdeI CTNAG 4 cut(s) 287, 316, 339, 977
DpnI GATC 4 cut(s) 165, 221, 424, 847
DpnII GATC 4 cut(s) 163, 219, 422, 845
DraI TTTAAA 1 cut(s) 748
Eam1104I CTCTTC 1 cut(s) 408
EarI CTCTTC 1 cut(s) 408
Ecl136II GAGCTC 1 cut(s) 358
Eco130I CCWWGG 3 cut(s) 383, 739, 982
Eco24I GRGCYC 1 cut(s) 360
Eco47I GGWCC 2 cut(s) 266, 548
Eco53kI GAGCTC 1 cut(s) 358
Eco57I CTGAAG 3 cut(s) 152, 224, 432
Eco88I CYCGRG 2 cut(s) 215, 449
Eco91I GGTNACC 1 cut(s) 725
EcoICRI GAGCTC 1 cut(s) 358
EcoNI CCTNNNNNAGG 1 cut(s) 950
EcoO109I RGGNCCY 1 cut(s) 266
EcoO65I GGTNACC 1 cut(s) 725
EcoRII CCWGG 2 cut(s) 72, 195
EcoT14I CCWWGG 3 cut(s) 383, 739, 982
EcoT38I GRGCYC 1 cut(s) 360
ErhI CCWWGG 3 cut(s) 383, 739, 982
FaeI CATG 4 cut(s) 307, 332, 709, 822
FaqI GGGAC 2 cut(s) 279, 882
FatI CATG 4 cut(s) 303, 328, 705, 818
FauNDI CATATG 1 cut(s) 694
FblI GTMKAC 1 cut(s) 250
Fnu4HI GCNGC 4 cut(s) 334, 513, 561, 645
FriOI GRGCYC 1 cut(s) 360
Fsp4HI GCNGC 4 cut(s) 334, 513, 561, 645
FspBI CTAG 1 cut(s) 672
GluI GCNGC 4 cut(s) 334, 513, 561, 645
GsuI CTGGAG 1 cut(s) 17
HaeIII GGCC 1 cut(s) 904
HapII CCGG 2 cut(s) 178, 602
HgaI GACGC 1 cut(s) 891
Hin1II CATG 4 cut(s) 307, 332, 709, 822
HindIII AAGCTT 1 cut(s) 474
HinfI GANTC 1 cut(s) 625
HpaII CCGG 2 cut(s) 178, 602
HphI GGTGA 2 cut(s) 530, 719
Hpy166II GTNNAC 4 cut(s) 115, 210, 251, 853
Hpy188I TCNGA 5 cut(s) 153, 171, 412, 624, 835
Hpy188III TCNNGA 5 cut(s) 34, 161, 215, 257, 602
Hpy8I GTNNAC 4 cut(s) 115, 210, 251, 853
Hpy99I CGWCG 2 cut(s) 885, 971
HpyAV CCTTC 2 cut(s) 577, 716
HpyCH4III ACNGT 1 cut(s) 618
HpyF10VI GCNNNNNNNGC 4 cut(s) 37, 140, 796, 978
HpyF3I CTNAG 4 cut(s) 287, 316, 339, 977
Hsp92II CATG 4 cut(s) 307, 332, 709, 822
Kpn2I TCCGGA 1 cut(s) 601
Kzo9I GATC 4 cut(s) 163, 219, 422, 845
LmnI GCTCC 2 cut(s) 36, 604
Lsp1109I GCAGC 4 cut(s) 320, 524, 547, 656
LweI GCATC 5 cut(s) 27, 121, 292, 808, 826
MaeI CTAG 1 cut(s) 672
MaeIII GTNAC 1 cut(s) 725
MalI GATC 4 cut(s) 165, 221, 424, 847
MbiI CCGCTC 1 cut(s) 31
MboI GATC 4 cut(s) 163, 219, 422, 845
MboII GAAGA 5 cut(s) 158, 356, 425, 744, 926
MflI RGATCY 1 cut(s) 422
MhlI GDGCHC 2 cut(s) 117, 360
MluCI AATT 6 cut(s) 45, 190, 239, 465, 827, 961
MroI TCCGGA 1 cut(s) 601
MroXI GAANNNNTTC 1 cut(s) 591
MseI TTAA 6 cut(s) 120, 189, 464, 579, 747, 804
MslI CAYNNNNRTG 2 cut(s) 804, 817
MspA1I CMGCKG 1 cut(s) 515
MspI CCGG 2 cut(s) 178, 602
MspR9I CCNGG 2 cut(s) 74, 197
MvaI CCWGG 2 cut(s) 74, 197
MwoI GCNNNNNNNGC 4 cut(s) 37, 140, 796, 978
NdeI CATATG 1 cut(s) 694
NdeII GATC 4 cut(s) 163, 219, 422, 845
NlaIII CATG 4 cut(s) 307, 332, 709, 822
NlaIV GGNNCC 2 cut(s) 267, 549
NmuCI GTSAC 1 cut(s) 725
NspI RCATGY 1 cut(s) 307
PciI ACATGT 1 cut(s) 303
PdmI GAANNNNTTC 1 cut(s) 591
PfeI GAWTC 1 cut(s) 625
PflFI GACNNNGTC 1 cut(s) 546
PkrI GCNGC 4 cut(s) 335, 514, 562, 646
PpuMI RGGWCCY 1 cut(s) 266
PscI ACATGT 1 cut(s) 303
PshBI ATTAAT 1 cut(s) 464
Psp124BI GAGCTC 1 cut(s) 360
Psp5II RGGWCCY 1 cut(s) 266
Psp6I CCWGG 2 cut(s) 72, 195
PspEI GGTNACC 1 cut(s) 725
PspGI CCWGG 2 cut(s) 72, 195
PspN4I GGNNCC 2 cut(s) 267, 549
PspPI GGNCC 2 cut(s) 266, 548
PspPPI RGGWCCY 1 cut(s) 266
PstNI CAGNNNCTG 1 cut(s) 202
PsuI RGATCY 1 cut(s) 422
PsyI GACNNNGTC 1 cut(s) 546
PvuII CAGCTG 1 cut(s) 515
RsaI GTAC 5 cut(s) 236, 293, 325, 490, 852
RsaNI GTAC 5 cut(s) 235, 292, 324, 489, 851
RseI CAYNNNNRTG 2 cut(s) 804, 817
SacI GAGCTC 1 cut(s) 360
SaqAI TTAA 6 cut(s) 120, 189, 464, 579, 747, 804
SatI GCNGC 4 cut(s) 334, 513, 561, 645
Sau3AI GATC 4 cut(s) 163, 219, 422, 845
Sau96I GGNCC 2 cut(s) 266, 548
ScrFI CCNGG 2 cut(s) 74, 197
SduI GDGCHC 2 cut(s) 117, 360
SfaNI GCATC 5 cut(s) 27, 121, 292, 808, 826
SinI GGWCC 2 cut(s) 266, 548
SmiMI CAYNNNNRTG 2 cut(s) 804, 817
SmlI CTYRAG 2 cut(s) 426, 991
SmoI CTYRAG 2 cut(s) 426, 991
Sse9I AATT 6 cut(s) 45, 190, 239, 465, 827, 961
SsiI CCGC 3 cut(s) 29, 551, 935
SspMI CTAG 1 cut(s) 672
SstI GAGCTC 1 cut(s) 360
StyD4I CCNGG 2 cut(s) 72, 195
StyI CCWWGG 3 cut(s) 383, 739, 982
TaaI ACNGT 1 cut(s) 618
TaqI TCGA 2 cut(s) 222, 880
TasI AATT 6 cut(s) 45, 190, 239, 465, 827, 961
TatI WGTACW 1 cut(s) 323
TfiI GAWTC 1 cut(s) 625
Tru1I TTAA 6 cut(s) 120, 189, 464, 579, 747, 804
Tru9I TTAA 6 cut(s) 120, 189, 464, 579, 747, 804
TscAI CASTG 2 cut(s) 207, 343
TseFI GTSAC 1 cut(s) 725
TseI GCWGC 4 cut(s) 333, 512, 560, 644
Tsp45I GTSAC 1 cut(s) 725
TspDTI ATGAA 2 cut(s) 621, 840
TspGWI ACGGA 2 cut(s) 506, 838
TspRI CASTG 2 cut(s) 207, 343
Tth111I GACNNNGTC 1 cut(s) 546
VneI GTGCAC 1 cut(s) 113
VpaK11BI GGWCC 2 cut(s) 266, 548
VspI ATTAAT 1 cut(s) 464
XagI CCTNNNNNAGG 1 cut(s) 950
XapI RAATTY 1 cut(s) 827
XceI RCATGY 1 cut(s) 307
XmiI GTMKAC 1 cut(s) 250
XmnI GAANNNNTTC 1 cut(s) 591
XspI CTAG 1 cut(s) 672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.