pycom16g07070

Protein of unknown function (DUF1336)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0008071
Physical Location & Seq
Reverse (-)
4583759 .. 4585881
2123 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1008 bp
ATGGGGGCGTGCGTGTCAAAGCCGGAGGGGTGCGTCGGAGGGAGATTGAGCTCGTCGAAGAGGAAGAGAACCCGGAAGAGGAGGAGAGACGGGCGGGCTAAACAGACTCCGGTTCTGGCCGGTCGGTTGTCGGAAGGATCGCCGGACAAGTTTAATCGGTCCACCCCGCCTGATCGCTCCACCTTCAACAACCGCACCTTCCAAGAAAGTACTGAAGACGCATGGTTTGATCCGGTTGCAAGATTTGAGTCTGACTGCGACGAAGATTTCCATAGTGTTCAGGATGAGGTGTTATCTGTGAATGGATTTGAACGCGTATCCGTATCAAGCAATTTGTCATTGAGAGATGCCAATTGCGGAGAGTATAATATTATTGACCTGCATGCCTCATCGACTGATCAGATGCATAAGAGAGGGGATTCTGCAAACAACTCTGTCAGTGTGGTTTCTCAAAAGAGCATTAATCATGTCATGAGTGGTAATGACGTTGATGGACATTCGACAGCTGAAGCAAACCAACCTGTGTTCCTCGATGAAATCTCCTCTTCTGTGGATGAATCTTCTACCAAGGAGGAAGGAATATTGGACAATTGTGGGATTCTTCCAAGCAATTGTCTGCCTTGTCTTGCTTCAACTGTTCCCTCGGTTGAAAAGAGAAGGTCACTGATTTCTAGCCCGCCAAGTGCAAGGAAAAAGGCTGCCATAAAACTTCCCTTCAAATGGAAAGAAGGACATCCTAACGCAAGTTTATTGTCCTCAAAGATGCTTCTGCAAAGACCAATAGCAGGTTCTCAAGTTCCTTTCTGCCCTATGGAAAAGAAAATGTTTGATTCTTGGTCACATATTGAGCCAAATTCTTTCAAAGTTCGGGGACCGAATTATTTTAAGGACAGAAAGAAGGAACATGCTCCCAGCTATGCTGCATATTATCCATTTGGTCTCGACGTATTTTTATCTCAGCGAAAGATAGATCGATATAGCTCGGTTTGTCGAACTGCCTGTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1336

Protein Analysis

336

Amino Acids

37.18

Weight (kDa)

8.8

Isoelectric Point (pI)

66.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR2_C PF07059 279 - 328 4e-10 Protein ENHANCED DISEASE RESISTANCE 2, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 387, 776
AccII CGCG 1 cut(s) 315
AciI CCGC 5 cut(s) 94, 167, 193, 357, 677
AclWI GGATC 2 cut(s) 145, 224
AcoI YGGCCR 1 cut(s) 117
AcsI RAATTY 1 cut(s) 853
AcuI CTGAAG 2 cut(s) 234, 528
AfaI GTAC 1 cut(s) 211
AfiI CCNNNNNNNGG 3 cut(s) 78, 720, 785
AflIII ACRYGT 1 cut(s) 313
AgsI TTSAA 6 cut(s) 187, 311, 633, 650, 718, 862
AjuI GAANNNNNNNTTGG 2 cut(s) 772, 804
AluBI AGCT 4 cut(s) 51, 506, 915, 981
AluI AGCT 4 cut(s) 51, 506, 915, 981
Alw21I GWGCWC 1 cut(s) 53
Alw26I GTCTC 2 cut(s) 81, 944
AlwI GGATC 2 cut(s) 145, 224
AoxI GGCC 1 cut(s) 117
ApeKI GCWGC 2 cut(s) 698, 920
ApoI RAATTY 1 cut(s) 853
ArsI GACNNNNNNTTYG 2 cut(s) 209, 241
AseI ATTAAT 1 cut(s) 462
AspS9I GGNCC 2 cut(s) 159, 872
AsuC2I CCSGG 1 cut(s) 73
AvaII GGWCC 2 cut(s) 159, 872
BanII GRGCYC 1 cut(s) 53
BbsI GAAGAC 1 cut(s) 222
Bbv12I GWGCWC 1 cut(s) 53
BbvI GCAGC 2 cut(s) 685, 907
BccI CCATC 1 cut(s) 485
BciVI GTATCC 1 cut(s) 328
BclI TGATCA 1 cut(s) 397
BcnI CCSGG 1 cut(s) 73
BcoDI GTCTC 2 cut(s) 81, 944
BfaI CTAG 1 cut(s) 672
BfuAI ACCTGC 2 cut(s) 387, 776
BfuI GTATCC 1 cut(s) 328
BisI GCNGC 2 cut(s) 699, 921
BlsI GCNGC 2 cut(s) 700, 922
BmcAI AGTACT 1 cut(s) 211
Bme1390I CCNGG 1 cut(s) 73
Bme18I GGWCC 2 cut(s) 159, 872
BmgT120I GGNCC 2 cut(s) 159, 872
BmiI GGNNCC 1 cut(s) 873
BmrFI CCNGG 1 cut(s) 73
BmsI GCATC 3 cut(s) 337, 393, 753
BpiI GAAGAC 1 cut(s) 222
BpuEI CTTGAG 1 cut(s) 777
BpuMI CCSGG 1 cut(s) 73
Bsa29I ATCGAT 1 cut(s) 973
BsaI GGTCTC 1 cut(s) 944
BsaJI CCNNGG 2 cut(s) 567, 642
BsaWI WCCGGW 2 cut(s) 109, 232
Bsc4I CCNNNNNNNGG 3 cut(s) 78, 720, 785
Bse118I RCCGGY 1 cut(s) 119
BseCI ATCGAT 1 cut(s) 973
BseDI CCNNGG 2 cut(s) 567, 642
BseGI GGATG 3 cut(s) 289, 559, 733
BseLI CCNNNNNNNGG 3 cut(s) 78, 720, 785
BseMII CTCAG 1 cut(s) 971
BseRI GAGGAG 3 cut(s) 94, 97, 532
BseXI GCAGC 2 cut(s) 685, 907
BseYI CCCAGC 1 cut(s) 911
Bsh1236I CGCG 1 cut(s) 315
Bsh1285I CGRYCG 1 cut(s) 124
BshFI GGCC 1 cut(s) 119
BshVI ATCGAT 1 cut(s) 973
BsiEI CGRYCG 1 cut(s) 124
BsiHKAI GWGCWC 1 cut(s) 53
BsiSI CCGG 6 cut(s) 23, 73, 110, 120, 143, 233
BslFI GGGAC 1 cut(s) 885
BslI CCNNNNNNNGG 3 cut(s) 78, 720, 785
BsmAI GTCTC 2 cut(s) 81, 944
BsmBI CGTCTC 1 cut(s) 81
BsmFI GGGAC 1 cut(s) 885
BsnI GGCC 1 cut(s) 119
Bso31I GGTCTC 1 cut(s) 944
Bsp1286I GDGCHC 1 cut(s) 53
Bsp143I GATC 5 cut(s) 137, 172, 229, 397, 970
BspACI CCGC 5 cut(s) 94, 167, 193, 357, 677
BspANI GGCC 1 cut(s) 119
BspCNI CTCAG 1 cut(s) 970
BspDI ATCGAT 1 cut(s) 973
BspFNI CGCG 1 cut(s) 315
BspHI TCATGA 1 cut(s) 471
BspLI GGNNCC 1 cut(s) 873
BspMI ACCTGC 2 cut(s) 387, 776
BspPI GGATC 2 cut(s) 145, 224
BspTNI GGTCTC 1 cut(s) 944
BsrFI RCCGGY 1 cut(s) 119
BssAI RCCGGY 1 cut(s) 119
BssECI CCNNGG 2 cut(s) 567, 642
BssMI GATC 5 cut(s) 137, 172, 229, 397, 970
BssT1I CCWWGG 1 cut(s) 567
Bst4CI ACNGT 1 cut(s) 637
Bst6I CTCTTC 4 cut(s) 53, 59, 71, 550
BstC8I GCNNGC 4 cut(s) 10, 96, 384, 677
BstDEI CTNAG 1 cut(s) 957
BstF5I GGATG 3 cut(s) 289, 559, 733
BstFNI CGCG 1 cut(s) 315
BstKTI GATC 5 cut(s) 140, 175, 232, 400, 973
BstMAI GTCTC 2 cut(s) 81, 944
BstMBI GATC 5 cut(s) 137, 172, 229, 397, 970
BstMCI CGRYCG 1 cut(s) 124
BstNSI RCATGY 2 cut(s) 386, 908
BstSCI CCNGG 1 cut(s) 71
BstUI CGCG 1 cut(s) 315
BstV1I GCAGC 2 cut(s) 685, 907
BstV2I GAAGAC 1 cut(s) 222
Bsu15I ATCGAT 1 cut(s) 973
BsuI GTATCC 1 cut(s) 328
BsuRI GGCC 1 cut(s) 119
BsuTUI ATCGAT 1 cut(s) 973
BtsCI GGATG 3 cut(s) 289, 559, 733
BtsIMutI CAGTG 2 cut(s) 445, 662
BveI ACCTGC 2 cut(s) 387, 776
Cac8I GCNNGC 4 cut(s) 10, 96, 384, 677
CciI TCATGA 1 cut(s) 471
Cfr10I RCCGGY 1 cut(s) 119
Cfr13I GGNCC 2 cut(s) 159, 872
ClaI ATCGAT 1 cut(s) 973
CseI GACGC 2 cut(s) 22, 227
Csp6I GTAC 1 cut(s) 210
CviAII CATG 5 cut(s) 222, 383, 467, 472, 905
CviQI GTAC 1 cut(s) 210
DdeI CTNAG 1 cut(s) 957
DpnI GATC 5 cut(s) 139, 174, 231, 399, 972
DpnII GATC 5 cut(s) 137, 172, 229, 397, 970
EaeI YGGCCR 1 cut(s) 117
Eam1104I CTCTTC 4 cut(s) 53, 59, 71, 550
EarI CTCTTC 4 cut(s) 53, 59, 71, 550
Ecl136II GAGCTC 1 cut(s) 51
Eco130I CCWWGG 1 cut(s) 567
Eco24I GRGCYC 1 cut(s) 53
Eco31I GGTCTC 1 cut(s) 944
Eco47I GGWCC 2 cut(s) 159, 872
Eco53kI GAGCTC 1 cut(s) 51
Eco57I CTGAAG 2 cut(s) 234, 528
EcoICRI GAGCTC 1 cut(s) 51
EcoT14I CCWWGG 1 cut(s) 567
EcoT22I ATGCAT 1 cut(s) 408
EcoT38I GRGCYC 1 cut(s) 53
ErhI CCWWGG 1 cut(s) 567
Esp3I CGTCTC 1 cut(s) 81
FaeI CATG 5 cut(s) 225, 386, 470, 475, 908
FaqI GGGAC 1 cut(s) 885
FatI CATG 5 cut(s) 221, 382, 466, 471, 904
FauI CCCGC 3 cut(s) 87, 174, 684
FbaI TGATCA 1 cut(s) 397
Fnu4HI GCNGC 2 cut(s) 699, 921
FokI GGATG 3 cut(s) 296, 566, 720
FriOI GRGCYC 1 cut(s) 53
Fsp4HI GCNGC 2 cut(s) 699, 921
FspBI CTAG 1 cut(s) 672
GluI GCNGC 2 cut(s) 699, 921
GsaI CCCAGC 1 cut(s) 915
HaeIII GGCC 1 cut(s) 119
HapII CCGG 6 cut(s) 23, 73, 110, 120, 143, 233
HgaI GACGC 2 cut(s) 22, 227
Hin1II CATG 5 cut(s) 225, 386, 470, 475, 908
HinfI GANTC 6 cut(s) 106, 248, 419, 557, 598, 830
HpaII CCGG 6 cut(s) 23, 73, 110, 120, 143, 233
Hpy166II GTNNAC 1 cut(s) 162
Hpy188I TCNGA 4 cut(s) 38, 133, 253, 402
Hpy188III TCNNGA 3 cut(s) 281, 472, 941
Hpy8I GTNNAC 1 cut(s) 162
Hpy99I CGWCG 4 cut(s) 38, 58, 263, 947
HpyAV CCTTC 8 cut(s) 128, 193, 208, 569, 651, 722, 724, 892
HpyCH4III ACNGT 1 cut(s) 637
HpyCH4IV ACGT 2 cut(s) 486, 945
HpyCH4V TGCA 7 cut(s) 239, 382, 406, 425, 686, 772, 923
HpyF3I CTNAG 1 cut(s) 957
HpySE526I ACGT 2 cut(s) 486, 945
Hsp92II CATG 5 cut(s) 225, 386, 470, 475, 908
Ksp22I TGATCA 1 cut(s) 397
Kzo9I GATC 5 cut(s) 137, 172, 229, 397, 970
LmnI GCTCC 2 cut(s) 182, 913
Lsp1109I GCAGC 2 cut(s) 685, 907
LweI GCATC 3 cut(s) 337, 393, 753
MaeI CTAG 1 cut(s) 672
MaeII ACGT 2 cut(s) 486, 945
MaeIII GTNAC 2 cut(s) 660, 837
MalI GATC 5 cut(s) 139, 174, 231, 399, 972
MboI GATC 5 cut(s) 137, 172, 229, 397, 970
MboII GAAGA 8 cut(s) 70, 76, 88, 227, 275, 537, 552, 593
MfeI CAATTG 3 cut(s) 352, 589, 610
MhlI GDGCHC 1 cut(s) 53
MluCI AATT 6 cut(s) 331, 352, 589, 610, 853, 877
MluI ACGCGT 1 cut(s) 313
MlyI GAGTC 2 cut(s) 100, 257
MmeI TCCRAC 2 cut(s) 16, 111
Mph1103I ATGCAT 1 cut(s) 408
MseI TTAA 4 cut(s) 153, 462, 885, 1006
MspA1I CMGCKG 1 cut(s) 506
MspI CCGG 6 cut(s) 23, 73, 110, 120, 143, 233
MspR9I CCNGG 1 cut(s) 73
MunI CAATTG 3 cut(s) 352, 589, 610
MvnI CGCG 1 cut(s) 315
NciI CCSGG 1 cut(s) 73
NdeII GATC 5 cut(s) 137, 172, 229, 397, 970
NlaIII CATG 5 cut(s) 225, 386, 470, 475, 908
NlaIV GGNNCC 1 cut(s) 873
NmuCI GTSAC 2 cut(s) 660, 837
NsiI ATGCAT 1 cut(s) 408
NspI RCATGY 2 cut(s) 386, 908
PaeI GCATGC 1 cut(s) 386
PagI TCATGA 1 cut(s) 471
PfeI GAWTC 4 cut(s) 419, 557, 598, 830
PkrI GCNGC 2 cut(s) 700, 922
PleI GAGTC 2 cut(s) 100, 256
PpsI GAGTC 2 cut(s) 100, 256
PshBI ATTAAT 1 cut(s) 462
Psp124BI GAGCTC 1 cut(s) 53
PspFI CCCAGC 1 cut(s) 911
PspN4I GGNNCC 1 cut(s) 873
PspPI GGNCC 2 cut(s) 159, 872
PvuII CAGCTG 1 cut(s) 506
RsaI GTAC 1 cut(s) 211
RsaNI GTAC 1 cut(s) 210
SacI GAGCTC 1 cut(s) 53
SaqAI TTAA 4 cut(s) 153, 462, 885, 1006
SatI GCNGC 2 cut(s) 699, 921
Sau3AI GATC 5 cut(s) 137, 172, 229, 397, 970
Sau96I GGNCC 2 cut(s) 159, 872
ScaI AGTACT 1 cut(s) 211
SchI GAGTC 2 cut(s) 100, 257
ScrFI CCNGG 1 cut(s) 73
SduI GDGCHC 1 cut(s) 53
SfaNI GCATC 3 cut(s) 337, 393, 753
SinI GGWCC 2 cut(s) 159, 872
SmlI CTYRAG 1 cut(s) 792
SmoI CTYRAG 1 cut(s) 792
SphI GCATGC 1 cut(s) 386
Sse9I AATT 6 cut(s) 331, 352, 589, 610, 853, 877
SsiI CCGC 5 cut(s) 94, 167, 193, 357, 677
SspI AATATT 2 cut(s) 370, 582
SspMI CTAG 1 cut(s) 672
SstI GAGCTC 1 cut(s) 53
StyD4I CCNGG 1 cut(s) 71
StyI CCWWGG 1 cut(s) 567
TaaI ACNGT 1 cut(s) 637
TaiI ACGT 2 cut(s) 489, 948
TaqI TCGA 7 cut(s) 56, 392, 500, 531, 942, 973, 991
TaqII GACCGA 2 cut(s) 147, 889
TasI AATT 6 cut(s) 331, 352, 589, 610, 853, 877
TatI WGTACW 1 cut(s) 209
TfiI GAWTC 4 cut(s) 419, 557, 598, 830
Tru1I TTAA 4 cut(s) 153, 462, 885, 1006
Tru9I TTAA 4 cut(s) 153, 462, 885, 1006
TscAI CASTG 2 cut(s) 445, 669
TseFI GTSAC 2 cut(s) 660, 837
TseI GCWGC 2 cut(s) 698, 920
Tsp45I GTSAC 2 cut(s) 660, 837
TspDTI ATGAA 2 cut(s) 549, 570
TspGWI ACGGA 1 cut(s) 310
TspRI CASTG 2 cut(s) 445, 669
VpaK11BI GGWCC 2 cut(s) 159, 872
VspI ATTAAT 1 cut(s) 462
XapI RAATTY 1 cut(s) 853
XceI RCATGY 2 cut(s) 386, 908
XspI CTAG 1 cut(s) 672
ZrmI AGTACT 1 cut(s) 211
Zsp2I ATGCAT 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.