pycom16g09340

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0011393
Physical Location & Seq
Reverse (-)
6260325 .. 6262013
1689 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1491 bp
ATGGCCTTGAATTTTTCTCACCGACCAATCTTTCCGGGGCATCTGTCTGAGGACAATTTGGTTTCTCCAATGAGGATTGCCAATGGGTACCTTGTGGAGGGTATGCCAGAGAGGAATGGAGATGGTTTCGGTAGGCCGTGGCATTCAAATCGAGAAGTGGATGGTTGCTTTGATTATGGAAGAGATAGATGTGATAGGGGTTGCTCACAGGATCCCGTTTCGAATGACATTCTTGATCTATTGCCTTCGGACCCTTTTGGCATGGATATAAGTACCACGTTTACGGCTATCACAGGCTGGCTTGAGGATTTGGAAGTGGACTACAGTGGTTACAGAAGGGATGAGGTGGGGACAAGTAACGGTAATTATGAACTTTTTGCAGGGTTGAATTTTATCTGGAATAATGCTATGAAGTTCCAGGCATTTCCAGGGAATATTGGGGTCGATCACAAGTCTAATGTAGCCAGTGGATTCGGTGGGTGCTCAAAAGAGAAGGGAGTTGATGATTCGCCCTTACACAGTGGTTTTGGATCAGCATGTCCTGTCGCTGACGTTTTGAATTTTGGCAATGAAAGTTGTGGTTTTTCCAATCGGCAAAATGTAGAGGTCCAGCATGGCGATGGGATTTGTGGTGGCGGTGATGGAGGAGCTCCTCATGGGGCTTTGATGTTTGCCCTTAGTTATCTTGGGGTGCAGGATCTTCTTGTTGTAGAAGGTGTTTGCAGATCTCTCCATTCTACAGTTCGCGGTGATCCCCTTTTGTGGAGAAATATTCACATAGATCAACCACTGAATGAGAAGATTACCGATGATGTTCTTTTGCAATTAACTGATAGGGCTCAAGGAAATCTGCAGAGCTTGAGCCTGGTGGAGTGCCCAAGGATCACTGATGATGGTCTGAAGCGTGTGCTTGAAAGTAATCCCAGGCTAGCCAAGTTGAGCGTGCCTGGATGTACTAGACTCAGTATTGAGGGCATTGTAAATAGCTTAAAGGTGTTCAATTCCAAGGGCGCACAAGGTGTGAAGCATTTACGAATTGGTGGACTCTATGGTGTGACACAGAAGCATTTTGAAGAGTTGAAGTTCTTGTTGGGAACTGATGGGCAGATGCAGCCTAATACACGCAAGCCACATTTTTATCAAAGAGGGAACTTTTATCTGGCCTCTGATGATGATAGTGACATTGATATTGAAATGTGCCCTCGATGCCAGAACCTGAGGCTTGTCTATGATTGCCCTGCTGAGGCTTGTCAAGGGAAAGAACATCCAACTCAGGTATGCAGGGCCTGCACACTTTGCATAGGGAGGTGTATTGAGTGTGGTCGGTGCATTAATGATAGTGAATATGAGGAAACGTTTTGTTTGGAGTTGCTTTGCTCAGATTGTTGGAAGCAGCTACTTAAGTCTCAAGAGACACAGGATGGAACCTACGGTCCCTCCAAACCTTCTGTTCTCCATGAACGGAATGATACTCTTAGTCGACATGGCTAG

Protein Analysis

497

Amino Acids

54.66

Weight (kDa)

5.13

Isoelectric Point (pI)

40.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 222 - 260 1e-05 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 87
AccB1I GGYRCC 1 cut(s) 87
AccI GTMKAC 1 cut(s) 1480
AccII CGCG 1 cut(s) 747
AciI CCGC 2 cut(s) 636, 747
AclI AACGTT 1 cut(s) 1355
AclWI GGATC 6 cut(s) 206, 219, 538, 705, 746, 890
AcsI RAATTY 3 cut(s) 10, 388, 559
AcuI CTGAAG 1 cut(s) 920
AdeI CACNNNGTG 1 cut(s) 1019
AfaI GTAC 3 cut(s) 89, 274, 955
AfiI CCNNNNNNNGG 3 cut(s) 97, 762, 1243
AflII CTTAAG 1 cut(s) 1400
AgsI TTSAA 9 cut(s) 10, 147, 388, 559, 914, 1000, 1073, 1081, 1193
AjnI CCWGG 5 cut(s) 417, 427, 864, 923, 946
AjuI GAANNNNNNNTTGG 2 cut(s) 1073, 1105
AloI GAACNNNNNNTCC 2 cut(s) 1417, 1449
AluBI AGCT 4 cut(s) 650, 858, 987, 1396
AluI AGCT 4 cut(s) 650, 858, 987, 1396
Alw21I GWGCWC 2 cut(s) 485, 652
Alw26I GTCTC 2 cut(s) 1406, 1410
AlwI GGATC 6 cut(s) 206, 219, 538, 705, 746, 890
AlwNI CAGNNNCTG 2 cut(s) 1216, 1287
AoxI GGCC 4 cut(s) 3, 134, 1161, 1284
ApeKI GCWGC 2 cut(s) 1111, 1393
ApoI RAATTY 3 cut(s) 10, 388, 559
AseI ATTAAT 1 cut(s) 1332
Asp718I GGTACC 1 cut(s) 87
AspLEI GCGC 1 cut(s) 1013
AspS9I GGNCC 4 cut(s) 250, 607, 1284, 1433
AsuC2I CCSGG 1 cut(s) 36
AsuHPI GGTGA 3 cut(s) 11, 650, 761
AsuII TTCGAA 1 cut(s) 221
AsuNHI GCTAGC 1 cut(s) 928
AvaII GGWCC 3 cut(s) 250, 607, 1433
AxyI CCTNAGG 1 cut(s) 1217
BaeGI GKGCMC 2 cut(s) 878, 1202
BamHI GGATCC 1 cut(s) 211
BanI GGYRCC 1 cut(s) 87
BanII GRGCYC 2 cut(s) 652, 841
Bbv12I GWGCWC 2 cut(s) 485, 652
BbvCI CCTCAGC 1 cut(s) 1242
BbvI GCAGC 2 cut(s) 1123, 1405
BccI CCATC 7 cut(s) 116, 155, 614, 635, 887, 1094, 1415
BceAI ACGGC 2 cut(s) 121, 300
BcgI CGANNNNNNTGC 2 cut(s) 131, 165
BciT130I CCWGG 5 cut(s) 419, 429, 866, 925, 948
BcnI CCSGG 1 cut(s) 36
BcoDI GTCTC 2 cut(s) 1406, 1410
BfaI CTAG 3 cut(s) 929, 957, 1489
BfmI CTRYAG 3 cut(s) 322, 738, 851
BfrI CTTAAG 1 cut(s) 1400
BglII AGATCT 1 cut(s) 725
BisI GCNGC 2 cut(s) 1112, 1394
BlsI GCNGC 2 cut(s) 1113, 1395
Bme1390I CCNGG 6 cut(s) 36, 419, 429, 866, 925, 948
Bme18I GGWCC 3 cut(s) 250, 607, 1433
BmgT120I GGNCC 4 cut(s) 250, 607, 1284, 1433
BmiI GGNNCC 5 cut(s) 89, 213, 252, 1426, 1435
BmrFI CCNGG 6 cut(s) 36, 419, 429, 866, 925, 948
BmsI GCATC 3 cut(s) 49, 1098, 1196
BmtI GCTAGC 1 cut(s) 932
Bpu10I CCTNAGC 1 cut(s) 1242
Bpu14I TTCGAA 1 cut(s) 221
BpuEI CTTGAG 4 cut(s) 323, 825, 880, 1392
BpuMI CCSGG 1 cut(s) 36
BsaJI CCNNGG 6 cut(s) 35, 137, 428, 878, 923, 1005
BsaXI ACNNNNNCTCC 2 cut(s) 1421, 1451
Bsc4I CCNNNNNNNGG 3 cut(s) 97, 762, 1243
Bse1I ACTGG 1 cut(s) 465
Bse21I CCTNAGG 1 cut(s) 1217
Bse3DI GCAATG 1 cut(s) 574
BseBI CCWGG 5 cut(s) 419, 429, 866, 925, 948
BseDI CCNNGG 6 cut(s) 35, 137, 428, 878, 923, 1005
BseGI GGATG 5 cut(s) 166, 346, 956, 1264, 1426
BseLI CCNNNNNNNGG 3 cut(s) 97, 762, 1243
BseMI GCAATG 1 cut(s) 574
BseMII CTCAG 6 cut(s) 39, 976, 1208, 1233, 1286, 1392
BseNI ACTGG 1 cut(s) 465
BseRI GAGGAG 2 cut(s) 642, 660
BseSI GKGCMC 2 cut(s) 878, 1202
BseXI GCAGC 2 cut(s) 1123, 1405
BsgI GTGCAG 2 cut(s) 713, 1273
Bsh1236I CGCG 1 cut(s) 747
BshFI GGCC 4 cut(s) 5, 136, 1163, 1286
BshNI GGYRCC 1 cut(s) 87
BsiHKAI GWGCWC 2 cut(s) 485, 652
BsiSI CCGG 1 cut(s) 35
BslFI GGGAC 2 cut(s) 364, 1419
BslI CCNNNNNNNGG 3 cut(s) 97, 762, 1243
BsmAI GTCTC 2 cut(s) 1406, 1410
BsmFI GGGAC 2 cut(s) 364, 1419
BsmI GAATGC 1 cut(s) 142
BsnI GGCC 4 cut(s) 5, 136, 1163, 1286
Bsp119I TTCGAA 1 cut(s) 221
Bsp1286I GDGCHC 5 cut(s) 485, 652, 841, 878, 1202
Bsp143I GATC 9 cut(s) 211, 235, 445, 530, 697, 725, 751, 781, 882
BspACI CCGC 2 cut(s) 636, 747
BspANI GGCC 4 cut(s) 5, 136, 1163, 1286
BspCNI CTCAG 6 cut(s) 40, 975, 1209, 1234, 1285, 1391
BspFNI CGCG 1 cut(s) 747
BspLI GGNNCC 5 cut(s) 89, 213, 252, 1426, 1435
BspMAI CTGCAG 1 cut(s) 855
BspOI GCTAGC 1 cut(s) 932
BspPI GGATC 6 cut(s) 206, 219, 538, 705, 746, 890
BspT104I TTCGAA 1 cut(s) 221
BspT107I GGYRCC 1 cut(s) 87
BspTI CTTAAG 1 cut(s) 1400
BsrDI GCAATG 1 cut(s) 574
BsrI ACTGG 1 cut(s) 465
BssECI CCNNGG 6 cut(s) 35, 137, 428, 878, 923, 1005
BssMI GATC 9 cut(s) 211, 235, 445, 530, 697, 725, 751, 781, 882
BssT1I CCWWGG 2 cut(s) 878, 1005
Bst2UI CCWGG 5 cut(s) 419, 429, 866, 925, 948
Bst4CI ACNGT 5 cut(s) 326, 362, 521, 742, 1433
Bst6I CTCTTC 2 cut(s) 175, 1068
BstAFI CTTAAG 1 cut(s) 1400
BstAPI GCANNNNNTGC 2 cut(s) 1287, 1296
BstBI TTCGAA 1 cut(s) 221
BstC8I GCNNGC 5 cut(s) 299, 930, 944, 1127, 1288
BstDEI CTNAG 8 cut(s) 48, 677, 962, 1217, 1242, 1272, 1378, 1475
BstDSI CCRYGG 1 cut(s) 137
BstENI CCTNNNNNAGG 1 cut(s) 95
BstF5I GGATG 5 cut(s) 166, 346, 956, 1264, 1426
BstFNI CGCG 1 cut(s) 747
BstHHI GCGC 1 cut(s) 1013
BstKTI GATC 9 cut(s) 214, 238, 448, 533, 700, 728, 754, 784, 885
BstMAI GTCTC 2 cut(s) 1406, 1410
BstMBI GATC 9 cut(s) 211, 235, 445, 530, 697, 725, 751, 781, 882
BstMWI GCNNNNNNNGC 4 cut(s) 1111, 1206, 1287, 1296
BstNI CCWGG 5 cut(s) 419, 429, 866, 925, 948
BstNSI RCATGY 1 cut(s) 540
BstSCI CCNGG 6 cut(s) 34, 417, 427, 864, 923, 946
BstSFI CTRYAG 3 cut(s) 322, 738, 851
BstSLI GKGCMC 2 cut(s) 878, 1202
BstUI CGCG 1 cut(s) 747
BstV1I GCAGC 2 cut(s) 1123, 1405
BstX2I RGATCY 3 cut(s) 211, 697, 725
BstYI RGATCY 3 cut(s) 211, 697, 725
Bsu36I CCTNAGG 1 cut(s) 1217
BsuRI GGCC 4 cut(s) 5, 136, 1163, 1286
BtgI CCRYGG 1 cut(s) 137
BtgZI GCGATG 1 cut(s) 633
BtsCI GGATG 5 cut(s) 166, 346, 956, 1264, 1426
BtsIMutI CAGTG 5 cut(s) 331, 472, 526, 788, 885
Cac8I GCNNGC 5 cut(s) 299, 930, 944, 1127, 1288
CaiI CAGNNNCTG 2 cut(s) 1216, 1287
CfoI GCGC 1 cut(s) 1013
Cfr13I GGNCC 4 cut(s) 250, 607, 1284, 1433
Csp6I GTAC 3 cut(s) 88, 273, 954
CviAII CATG 6 cut(s) 262, 537, 614, 656, 1457, 1484
CviQI GTAC 3 cut(s) 88, 273, 954
DdeI CTNAG 8 cut(s) 48, 677, 962, 1217, 1242, 1272, 1378, 1475
DpnI GATC 9 cut(s) 213, 237, 447, 532, 699, 727, 753, 783, 884
DpnII GATC 9 cut(s) 211, 235, 445, 530, 697, 725, 751, 781, 882
DraIII CACNNNGTG 1 cut(s) 1019
Eam1104I CTCTTC 2 cut(s) 175, 1068
EarI CTCTTC 2 cut(s) 175, 1068
Ecl136II GAGCTC 1 cut(s) 650
Eco130I CCWWGG 2 cut(s) 878, 1005
Eco24I GRGCYC 2 cut(s) 652, 841
Eco47I GGWCC 3 cut(s) 250, 607, 1433
Eco53kI GAGCTC 1 cut(s) 650
Eco57I CTGAAG 1 cut(s) 920
Eco81I CCTNAGG 1 cut(s) 1217
EcoICRI GAGCTC 1 cut(s) 650
EcoNI CCTNNNNNAGG 1 cut(s) 95
EcoO109I RGGNCCY 1 cut(s) 1284
EcoRII CCWGG 5 cut(s) 417, 427, 864, 923, 946
EcoT14I CCWWGG 2 cut(s) 878, 1005
EcoT38I GRGCYC 2 cut(s) 652, 841
ErhI CCWWGG 2 cut(s) 878, 1005
FaeI CATG 6 cut(s) 265, 540, 617, 659, 1460, 1487
FaqI GGGAC 2 cut(s) 364, 1419
FatI CATG 6 cut(s) 261, 536, 613, 655, 1456, 1483
FblI GTMKAC 1 cut(s) 1480
Fnu4HI GCNGC 2 cut(s) 1112, 1394
FokI GGATG 5 cut(s) 173, 353, 963, 1251, 1433
FriOI GRGCYC 2 cut(s) 652, 841
Fsp4HI GCNGC 2 cut(s) 1112, 1394
FspBI CTAG 3 cut(s) 929, 957, 1489
GlaI GCGC 1 cut(s) 1012
GluI GCNGC 2 cut(s) 1112, 1394
HaeIII GGCC 4 cut(s) 5, 136, 1163, 1286
HapII CCGG 1 cut(s) 35
HhaI GCGC 1 cut(s) 1013
Hin1II CATG 6 cut(s) 265, 540, 617, 659, 1460, 1487
Hin6I GCGC 1 cut(s) 1011
HinP1I GCGC 1 cut(s) 1011
HincII GTYRAC 1 cut(s) 1481
HindII GTYRAC 1 cut(s) 1481
HinfI GANTC 4 cut(s) 471, 506, 960, 1044
HpaII CCGG 1 cut(s) 35
HphI GGTGA 3 cut(s) 11, 650, 761
Hpy166II GTNNAC 4 cut(s) 282, 319, 1043, 1481
Hpy188I TCNGA 5 cut(s) 49, 250, 900, 1168, 1381
Hpy188III TCNNGA 4 cut(s) 152, 233, 397, 1409
Hpy8I GTNNAC 4 cut(s) 282, 319, 1043, 1481
HpyAV CCTTC 5 cut(s) 255, 330, 487, 707, 1455
HpyCH4III ACNGT 5 cut(s) 326, 362, 521, 742, 1433
HpyCH4IV ACGT 3 cut(s) 278, 552, 1355
HpyF10VI GCNNNNNNNGC 4 cut(s) 1111, 1206, 1287, 1296
HpyF3I CTNAG 8 cut(s) 48, 677, 962, 1217, 1242, 1272, 1378, 1475
HpySE526I ACGT 3 cut(s) 278, 552, 1355
Hsp92II CATG 6 cut(s) 265, 540, 617, 659, 1460, 1487
HspAI GCGC 1 cut(s) 1011
KpnI GGTACC 1 cut(s) 91
Kzo9I GATC 9 cut(s) 211, 235, 445, 530, 697, 725, 751, 781, 882
LmnI GCTCC 2 cut(s) 647, 655
Lsp1109I GCAGC 2 cut(s) 1123, 1405
LweI GCATC 3 cut(s) 49, 1098, 1196
MaeI CTAG 3 cut(s) 929, 957, 1489
MaeII ACGT 3 cut(s) 278, 552, 1355
MaeIII GTNAC 4 cut(s) 329, 356, 1054, 1178
MalI GATC 9 cut(s) 213, 237, 447, 532, 699, 727, 753, 783, 884
MboI GATC 9 cut(s) 211, 235, 445, 530, 697, 725, 751, 781, 882
MboII GAAGA 4 cut(s) 192, 692, 811, 1085
MflI RGATCY 3 cut(s) 211, 697, 725
MhlI GDGCHC 5 cut(s) 485, 652, 841, 878, 1202
MluCI AATT 8 cut(s) 10, 55, 364, 388, 559, 824, 1000, 1035
MlyI GAGTC 2 cut(s) 954, 1038
MmeI TCCRAC 2 cut(s) 1292, 1367
MseI TTAA 4 cut(s) 827, 989, 1332, 1401
MslI CAYNNNNRTG 1 cut(s) 618
MspCI CTTAAG 1 cut(s) 1400
MspI CCGG 1 cut(s) 35
MspR9I CCNGG 6 cut(s) 36, 419, 429, 866, 925, 948
Mva1269I GAATGC 1 cut(s) 142
MvaI CCWGG 5 cut(s) 419, 429, 866, 925, 948
MvnI CGCG 1 cut(s) 747
MwoI GCNNNNNNNGC 4 cut(s) 1111, 1206, 1287, 1296
NciI CCSGG 1 cut(s) 36
NdeII GATC 9 cut(s) 211, 235, 445, 530, 697, 725, 751, 781, 882
NheI GCTAGC 1 cut(s) 928
NlaIII CATG 6 cut(s) 265, 540, 617, 659, 1460, 1487
NlaIV GGNNCC 5 cut(s) 89, 213, 252, 1426, 1435
NmuCI GTSAC 2 cut(s) 1054, 1178
NspI RCATGY 1 cut(s) 540
NspV TTCGAA 1 cut(s) 221
PctI GAATGC 1 cut(s) 142
PfeI GAWTC 2 cut(s) 471, 506
PkrI GCNGC 2 cut(s) 1113, 1395
PleI GAGTC 2 cut(s) 954, 1038
PpsI GAGTC 2 cut(s) 954, 1038
PshBI ATTAAT 1 cut(s) 1332
Psp124BI GAGCTC 1 cut(s) 652
Psp1406I AACGTT 1 cut(s) 1355
Psp6I CCWGG 5 cut(s) 417, 427, 864, 923, 946
PspGI CCWGG 5 cut(s) 417, 427, 864, 923, 946
PspN4I GGNNCC 5 cut(s) 89, 213, 252, 1426, 1435
PspPI GGNCC 4 cut(s) 250, 607, 1284, 1433
PstI CTGCAG 1 cut(s) 855
PstNI CAGNNNCTG 2 cut(s) 1216, 1287
PsuI RGATCY 3 cut(s) 211, 697, 725
RsaI GTAC 3 cut(s) 89, 274, 955
RsaNI GTAC 3 cut(s) 88, 273, 954
RseI CAYNNNNRTG 1 cut(s) 618
SacI GAGCTC 1 cut(s) 652
SalI GTCGAC 1 cut(s) 1479
SaqAI TTAA 4 cut(s) 827, 989, 1332, 1401
SatI GCNGC 2 cut(s) 1112, 1394
Sau3AI GATC 9 cut(s) 211, 235, 445, 530, 697, 725, 751, 781, 882
Sau96I GGNCC 4 cut(s) 250, 607, 1284, 1433
SchI GAGTC 2 cut(s) 954, 1038
ScrFI CCNGG 6 cut(s) 36, 419, 429, 866, 925, 948
SduI GDGCHC 5 cut(s) 485, 652, 841, 878, 1202
SfaNI GCATC 3 cut(s) 49, 1098, 1196
SfcI CTRYAG 3 cut(s) 322, 738, 851
SfuI TTCGAA 1 cut(s) 221
SinI GGWCC 3 cut(s) 250, 607, 1433
SmiMI CAYNNNNRTG 1 cut(s) 618
SmlI CTYRAG 5 cut(s) 302, 840, 859, 1400, 1407
SmoI CTYRAG 5 cut(s) 302, 840, 859, 1400, 1407
Sse9I AATT 8 cut(s) 10, 55, 364, 388, 559, 824, 1000, 1035
SsiI CCGC 2 cut(s) 636, 747
SspI AATATT 2 cut(s) 436, 772
SspMI CTAG 3 cut(s) 929, 957, 1489
SstI GAGCTC 1 cut(s) 652
StyD4I CCNGG 6 cut(s) 34, 417, 427, 864, 923, 946
StyI CCWWGG 2 cut(s) 878, 1005
TaaI ACNGT 5 cut(s) 326, 362, 521, 742, 1433
TaiI ACGT 3 cut(s) 281, 555, 1358
TaqI TCGA 5 cut(s) 151, 221, 444, 1204, 1480
TasI AATT 8 cut(s) 10, 55, 364, 388, 559, 824, 1000, 1035
TatI WGTACW 1 cut(s) 953
TfiI GAWTC 2 cut(s) 471, 506
Tru1I TTAA 4 cut(s) 827, 989, 1332, 1401
Tru9I TTAA 4 cut(s) 827, 989, 1332, 1401
TscAI CASTG 5 cut(s) 331, 472, 526, 795, 892
TseFI GTSAC 2 cut(s) 1054, 1178
TseI GCWGC 2 cut(s) 1111, 1393
Tsp45I GTSAC 2 cut(s) 1054, 1178
TspDTI ATGAA 4 cut(s) 384, 425, 585, 1473
TspGWI ACGGA 1 cut(s) 1477
TspRI CASTG 5 cut(s) 331, 472, 526, 795, 892
Vha464I CTTAAG 1 cut(s) 1400
VpaK11BI GGWCC 3 cut(s) 250, 607, 1433
VspI ATTAAT 1 cut(s) 1332
XagI CCTNNNNNAGG 1 cut(s) 95
XapI RAATTY 3 cut(s) 10, 388, 559
XceI RCATGY 1 cut(s) 540
XcmI CCANNNNNNNNNTGG 1 cut(s) 617
XmiI GTMKAC 1 cut(s) 1480
XspI CTAG 3 cut(s) 929, 957, 1489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.