Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0016608

Physical Location & Seq

Genomic Coordinates Reverse (-)

6841650 .. 6844367

Gene Structure 2718 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1200 bp

ATGTTCGGAGCTCAATCTTCGTCGTCCCCCTTCGGCACGCCTTCGTCAACCCCAGCGTTCGGGACGCCGTCGTCAACCCCAGCCTTCGGGACGCCGTCGTCGACCCCGGCTTTCGGGACGCCGTCGTCGACCCCGGCATTCGGGACGCCATCTTCGACGCCATCGTTCTTTACTCCTTCGACCCCGGCCTTTGGAACTCCGTCGACGTCTGCATTTTCCGCCGGTGGCTTCGGCGGCTCCTTATTCAACACTCCGTTCTCTTCTCAAACTCAGCAGCAACAACAACAGCAGCAAACGCCGTCGTTTCAGCAGCCTCAGTCTGCTGGCGGCTTTGGGTTTCAGGCTCCGTTCTCTGCGCCGCAGCAGCCGACGCCCTTCCCTAATTCTCAATTGACGACTCAGATGGCTCCTGTCGCTCCTCTCCCTTTCTCTCTCGCCGATCGCGATATTCAAGCAATTGTTGATGCTTACAAGGACGAGCCTGGAAACCCTAAATATGCTTTTAAGCATTTGCTGTTTAGCGTAACTGATCCGCAGTTCAGGGTGAAGCCTGCTGGTGTATCGGATATAATGTGGGCAGAGGCTATGGCGAAGCTTGAAGGTCTGGAAAGTACTGACAGGGAACGCCTGTGGCCTCAGCTTGTTCAGGGTTTTAAAGATCTCTCTCAACGCCTGAAGCTCCAAGATGAAGTCCTTCTTTCAGATGCTGAGAGATTGCAAATGACCCAAAGCAATGTCAAGATGCTTCAAAGGCATTTTCAAGCTGACACTCTTCCTTGGATCGAAAGAATGAGGCAAAAAGAACAAGGTCTTCAAAGGCGCCTTTTGAGGGTGATGAGAATACTGGAGGCACTAGAGGGAAAGGGTTTCCGACTGCCTTTAATGAAAGGTGAAGTAGAATTGGCCGAGAAGTTAGCTGCAGTTACAAGACAGCTGAAAGGATCGGGAGCTGAACTTTCTAGGAGGGTACAAAACCTATTAACCATATCCCGTGTCCAAGAGAATTCTATTGGTGGCGGTGGTTTAGGTTATCTTCCAGGATCAACCAAAATACACGAACAGAGTCTTGCTGCTATGCAAGAGGTGTTACAGCAGCAGACGGAAGCTATTGCGAGACTCGGCAATGTTTTGAAGCGAGATATTAGGGATATGGAGATTATAATGGCTGAGGATATGGAAACAACACAGAACGGGAGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0005730 GO:0006606 GO:0006607 GO:0006810 GO:0006886 GO:0006913 GO:0006996 GO:0006997 GO:0006999 GO:0008104 GO:0008150 GO:0009987 GO:0012505 GO:0015031 GO:0015833 GO:0016043 GO:0017038 GO:0017056 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032991 GO:0033036 GO:0033365 GO:0034504 GO:0034613 GO:0036228 GO:0042886 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044613 GO:0045184 GO:0046907 GO:0051169 GO:0051170 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070013 GO:0070727 GO:0071702 GO:0071705 GO:0071840 GO:0072594 GO:0072657 GO:0090435

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

Nup54

Protein Analysis

400

Amino Acids

43.48

Weight (kDa)

6.88

Isoelectric Point (pI)

55.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Nup54	PF13874	183 - 327	3.5e-31	Nucleoporin complex subunit 54

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0016608)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G24310
fragaria_vesca	FvH4_4g23970
malus_domestica	MD13G1116800.v1.1 MD16G1117500.v1.1
prunus_persica	Prupe.1G228700_v2.0.a1
pyrus_communis	pycom13g10140 pycom16g10070
rosa_chinensis	RchiOBHm_Chr4g0430621
rosa_laevigata	RLG00000006962
rosa_multiflora	Rmu_sc0000131.1_g000016
rosa_roxburghii	Rroxscaffold_5G00372370
rosa_rugosa	Rorug04G0244800
rosa_samantha	Rh4DG305500
rosa_wichuraiana	Rw4G026100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1160
AasI	GACNNNNNNGTC	3 cut(s)	70, 97, 124
AatII	GACGTC	1 cut(s)	209
AccB1I	GGYRCC	1 cut(s)	819
AccI	GTMKAC	3 cut(s)	101, 128, 203
AccII	CGCG	1 cut(s)	444
AciI	CCGC	6 cut(s)	219, 234, 327, 359, 533, 1017
AclWI	GGATC	4 cut(s)	524, 788, 949, 1048
AcoI	YGGCCR	1 cut(s)	903
AcsI	RAATTY	1 cut(s)	1003
AcuI	CTGAAG	1 cut(s)	695
AcyI	GRCGYC	8 cut(s)	65, 92, 119, 146, 158, 206, 371, 820
AfaI	GTAC	2 cut(s)	613, 969
AfiI	CCNNNNNNNGG	6 cut(s)	59, 86, 113, 140, 191, 829
AgsI	TTSAA	7 cut(s)	247, 452, 599, 749, 761, 815, 1132
AjnI	CCWGG	2 cut(s)	481, 1036
Alw21I	GWGCWC	1 cut(s)	13
Alw26I	GTCTC	1 cut(s)	1108
AlwI	GGATC	4 cut(s)	524, 788, 949, 1048
AlwNI	CAGNNNCTG	1 cut(s)	707
AoxI	GGCC	3 cut(s)	186, 632, 903
ApeKI	GCWGC	8 cut(s)	274, 289, 310, 361, 364, 917, 1070, 1093
ApoI	RAATTY	1 cut(s)	1003
ArsI	GACNNNNNNTTYG	2 cut(s)	136, 168
Asp700I	GAANNNNTTC	1 cut(s)	693
AspLEI	GCGC	2 cut(s)	358, 822
AsuC2I	CCSGG	3 cut(s)	107, 134, 185
AsuHPI	GGTGA	3 cut(s)	556, 844, 902
BanI	GGYRCC	1 cut(s)	819
BanII	GRGCYC	1 cut(s)	13
BbsI	GAAGAC	1 cut(s)	803
Bbv12I	GWGCWC	1 cut(s)	13
BbvCI	CCTCAGC	2 cut(s)	636, 1167
BbvI	GCAGC	8 cut(s)	286, 301, 322, 373, 376, 904, 1057, 1105
BccI	CCATC	3 cut(s)	157, 169, 397
BceAI	ACGGC	4 cut(s)	52, 79, 106, 283
BciT130I	CCWGG	2 cut(s)	483, 1038
BcnI	CCSGG	3 cut(s)	107, 134, 185
BcoDI	GTCTC	1 cut(s)	1108
BfaI	CTAG	2 cut(s)	854, 960
BfmI	CTRYAG	1 cut(s)	918
BfoI	RGCGCY	1 cut(s)	823
BglII	AGATCT	1 cut(s)	658
BmcAI	AGTACT	1 cut(s)	613
Bme1390I	CCNGG	5 cut(s)	107, 134, 185, 483, 1038
BmiI	GGNNCC	4 cut(s)	238, 345, 408, 821
BmrFI	CCNGG	5 cut(s)	107, 134, 185, 483, 1038
BmsI	GCATC	3 cut(s)	454, 694, 732
BpiI	GAAGAC	1 cut(s)	803
BpmI	CTGGAG	1 cut(s)	866
Bpu10I	CCTNAGC	2 cut(s)	636, 1167
BpuMI	CCSGG	3 cut(s)	107, 134, 185
BsaHI	GRCGYC	8 cut(s)	65, 92, 119, 146, 158, 206, 371, 820
BsaJI	CCNNGG	4 cut(s)	105, 132, 183, 776
Bsc4I	CCNNNNNNNGG	6 cut(s)	59, 86, 113, 140, 191, 829
Bse118I	RCCGGY	1 cut(s)	221
Bse1I	ACTGG	1 cut(s)	849
Bse3DI	GCAATG	2 cut(s)	739, 1129
BseBI	CCWGG	2 cut(s)	483, 1038
BseDI	CCNNGG	4 cut(s)	105, 132, 183, 776
BseLI	CCNNNNNNNGG	6 cut(s)	59, 86, 113, 140, 191, 829
BseMI	GCAATG	2 cut(s)	739, 1129
BseMII	CTCAG	6 cut(s)	284, 329, 413, 650, 699, 1158
BseNI	ACTGG	1 cut(s)	849
BseRI	GAGGAG	1 cut(s)	408
BseXI	GCAGC	8 cut(s)	286, 301, 322, 373, 376, 904, 1057, 1105
BseYI	CCCAGC	2 cut(s)	52, 79
Bsh1236I	CGCG	1 cut(s)	444
Bsh1285I	CGRYCG	1 cut(s)	442
BshFI	GGCC	3 cut(s)	188, 634, 905
BshNI	GGYRCC	1 cut(s)	819
BsiEI	CGRYCG	1 cut(s)	442
BsiHKAI	GWGCWC	1 cut(s)	13
BsiSI	CCGG	4 cut(s)	107, 134, 185, 222
BslFI	GGGAC	5 cut(s)	10, 76, 103, 130, 157
BslI	CCNNNNNNNGG	6 cut(s)	59, 86, 113, 140, 191, 829
BsmAI	GTCTC	1 cut(s)	1108
BsmFI	GGGAC	5 cut(s)	10, 76, 103, 130, 157
BsmI	GAATGC	1 cut(s)	137
BsnI	GGCC	3 cut(s)	188, 634, 905
Bsp1286I	GDGCHC	1 cut(s)	13
Bsp143I	GATC	6 cut(s)	439, 529, 658, 780, 941, 1040
Bsp68I	TCGCGA	1 cut(s)	444
BspACI	CCGC	6 cut(s)	219, 234, 327, 359, 533, 1017
BspANI	GGCC	3 cut(s)	188, 634, 905
BspCNI	CTCAG	6 cut(s)	283, 328, 412, 649, 700, 1159
BspFNI	CGCG	1 cut(s)	444
BspLI	GGNNCC	4 cut(s)	238, 345, 408, 821
BspMAI	CTGCAG	1 cut(s)	922
BspPI	GGATC	4 cut(s)	524, 788, 949, 1048
BspT107I	GGYRCC	1 cut(s)	819
BsrDI	GCAATG	2 cut(s)	739, 1129
BsrFI	RCCGGY	1 cut(s)	221
BsrI	ACTGG	1 cut(s)	849
BssAI	RCCGGY	1 cut(s)	221
BssECI	CCNNGG	4 cut(s)	105, 132, 183, 776
BssMI	GATC	6 cut(s)	439, 529, 658, 780, 941, 1040
BssNI	GRCGYC	8 cut(s)	65, 92, 119, 146, 158, 206, 371, 820
BssT1I	CCWWGG	1 cut(s)	776
Bst2UI	CCWGG	2 cut(s)	483, 1038
Bst6I	CTCTTC	2 cut(s)	265, 777
BstACI	GRCGYC	8 cut(s)	65, 92, 119, 146, 158, 206, 371, 820
BstC8I	GCNNGC	3 cut(s)	38, 325, 552
BstDEI	CTNAG	6 cut(s)	270, 315, 399, 636, 708, 1167
BstFNI	CGCG	1 cut(s)	444
BstH2I	RGCGCY	1 cut(s)	823
BstHHI	GCGC	2 cut(s)	358, 822
BstKTI	GATC	6 cut(s)	442, 532, 661, 783, 944, 1043
BstMAI	GTCTC	1 cut(s)	1108
BstMBI	GATC	6 cut(s)	439, 529, 658, 780, 941, 1040
BstMCI	CGRYCG	1 cut(s)	442
BstMWI	GCNNNNNNNGC	7 cut(s)	218, 234, 295, 364, 370, 413, 751
BstNI	CCWGG	2 cut(s)	483, 1038
BstSCI	CCNGG	5 cut(s)	105, 132, 183, 481, 1036
BstSFI	CTRYAG	1 cut(s)	918
BstUI	CGCG	1 cut(s)	444
BstV1I	GCAGC	8 cut(s)	286, 301, 322, 373, 376, 904, 1057, 1105
BstV2I	GAAGAC	1 cut(s)	803
BstX2I	RGATCY	1 cut(s)	658
BstYI	RGATCY	1 cut(s)	658
BsuRI	GGCC	3 cut(s)	188, 634, 905
BtuMI	TCGCGA	1 cut(s)	444
Cac8I	GCNNGC	3 cut(s)	38, 325, 552
CaiI	CAGNNNCTG	1 cut(s)	707
CfoI	GCGC	2 cut(s)	358, 822
Cfr10I	RCCGGY	1 cut(s)	221
CseI	GACGC	6 cut(s)	73, 100, 127, 154, 166, 379
Csp6I	GTAC	2 cut(s)	612, 968
CviQI	GTAC	2 cut(s)	612, 968
DdeI	CTNAG	6 cut(s)	270, 315, 399, 636, 708, 1167
DinI	GGCGCC	1 cut(s)	821
DpnI	GATC	6 cut(s)	441, 531, 660, 782, 943, 1042
DpnII	GATC	6 cut(s)	439, 529, 658, 780, 941, 1040
DraI	TTTAAA	1 cut(s)	655
DrdI	GACNNNNNNGTC	3 cut(s)	70, 97, 124
DseDI	GACNNNNNNGTC	3 cut(s)	70, 97, 124
EaeI	YGGCCR	1 cut(s)	903
Eam1104I	CTCTTC	2 cut(s)	265, 777
EarI	CTCTTC	2 cut(s)	265, 777
EciI	GGCGGA	1 cut(s)	208
Ecl136II	GAGCTC	1 cut(s)	11
Eco130I	CCWWGG	1 cut(s)	776
Eco24I	GRGCYC	1 cut(s)	13
Eco53kI	GAGCTC	1 cut(s)	11
Eco57I	CTGAAG	1 cut(s)	695
EcoICRI	GAGCTC	1 cut(s)	11
EcoRI	GAATTC	1 cut(s)	1003
EcoRII	CCWGG	2 cut(s)	481, 1036
EcoT14I	CCWWGG	1 cut(s)	776
EcoT38I	GRGCYC	1 cut(s)	13
EgeI	GGCGCC	1 cut(s)	821
EheI	GGCGCC	1 cut(s)	821
ErhI	CCWWGG	1 cut(s)	776
FaiI	YATR	8 cut(s)	498, 569, 587, 986, 1076, 1151, 1160, 1175
FalI	AAGNNNNNCTT	2 cut(s)	681, 713
FaqI	GGGAC	5 cut(s)	10, 76, 103, 130, 157
FblI	GTMKAC	3 cut(s)	101, 128, 203
FriOI	GRGCYC	1 cut(s)	13
FspBI	CTAG	2 cut(s)	854, 960
GlaI	GCGC	2 cut(s)	357, 821
GsaI	CCCAGC	2 cut(s)	56, 83
GsuI	CTGGAG	1 cut(s)	866
HaeII	RGCGCY	1 cut(s)	823
HaeIII	GGCC	3 cut(s)	188, 634, 905
HapII	CCGG	4 cut(s)	107, 134, 185, 222
HgaI	GACGC	6 cut(s)	73, 100, 127, 154, 166, 379
HhaI	GCGC	2 cut(s)	358, 822
Hin1I	GRCGYC	8 cut(s)	65, 92, 119, 146, 158, 206, 371, 820
Hin6I	GCGC	2 cut(s)	356, 820
HinP1I	GCGC	2 cut(s)	356, 820
HincII	GTYRAC	5 cut(s)	48, 75, 102, 129, 204
HindII	GTYRAC	5 cut(s)	48, 75, 102, 129, 204
HindIII	AAGCTT	1 cut(s)	593
HinfI	GANTC	3 cut(s)	397, 1063, 1116
HpaII	CCGG	4 cut(s)	107, 134, 185, 222
HphI	GGTGA	3 cut(s)	556, 844, 902
Hpy166II	GTNNAC	5 cut(s)	48, 75, 102, 129, 204
Hpy188I	TCNGA	5 cut(s)	8, 402, 565, 703, 872
Hpy188III	TCNNGA	8 cut(s)	61, 88, 115, 142, 443, 605, 739, 945
Hpy8I	GTNNAC	5 cut(s)	48, 75, 102, 129, 204
HpyAV	CCTTC	7 cut(s)	40, 51, 94, 186, 385, 593, 704
HpyCH4IV	ACGT	1 cut(s)	206
HpyCH4V	TGCA	4 cut(s)	212, 718, 920, 1078
HpyF10VI	GCNNNNNNNGC	7 cut(s)	218, 234, 295, 364, 370, 413, 751
HpyF3I	CTNAG	6 cut(s)	270, 315, 399, 636, 708, 1167
HpySE526I	ACGT	1 cut(s)	206
Hsp92I	GRCGYC	8 cut(s)	65, 92, 119, 146, 158, 206, 371, 820
HspAI	GCGC	2 cut(s)	356, 820
KasI	GGCGCC	1 cut(s)	819
Kzo9I	GATC	6 cut(s)	439, 529, 658, 780, 941, 1040
LmnI	GCTCC	8 cut(s)	8, 242, 349, 412, 421, 684, 947, 1194
Lsp1109I	GCAGC	8 cut(s)	286, 301, 322, 373, 376, 904, 1057, 1105
LweI	GCATC	3 cut(s)	454, 694, 732
MaeI	CTAG	2 cut(s)	854, 960
MaeII	ACGT	1 cut(s)	206
MaeIII	GTNAC	3 cut(s)	523, 922, 1086
MalI	GATC	6 cut(s)	441, 531, 660, 782, 943, 1042
MboI	GATC	6 cut(s)	439, 529, 658, 780, 941, 1040
MboII	GAAGA	6 cut(s)	9, 144, 252, 764, 803, 1025
MfeI	CAATTG	2 cut(s)	389, 456
MflI	RGATCY	1 cut(s)	658
MhlI	GDGCHC	1 cut(s)	13
MluCI	AATT	5 cut(s)	382, 389, 456, 899, 1003
Mly113I	GGCGCC	1 cut(s)	820
MlyI	GAGTC	3 cut(s)	391, 1072, 1110
MmeI	TCCRAC	1 cut(s)	895
MroXI	GAANNNNTTC	1 cut(s)	693
MseI	TTAA	4 cut(s)	504, 654, 881, 980
MspA1I	CMGCKG	1 cut(s)	934
MspI	CCGG	4 cut(s)	107, 134, 185, 222
MspR9I	CCNGG	5 cut(s)	107, 134, 185, 483, 1038
MunI	CAATTG	2 cut(s)	389, 456
Mva1269I	GAATGC	1 cut(s)	137
MvaI	CCWGG	2 cut(s)	483, 1038
MvnI	CGCG	1 cut(s)	444
MwoI	GCNNNNNNNGC	7 cut(s)	218, 234, 295, 364, 370, 413, 751
NarI	GGCGCC	1 cut(s)	820
NciI	CCSGG	3 cut(s)	107, 134, 185
NdeII	GATC	6 cut(s)	439, 529, 658, 780, 941, 1040
NlaIV	GGNNCC	4 cut(s)	238, 345, 408, 821
NmeAIII	GCCGAG	2 cut(s)	931, 1098
NruI	TCGCGA	1 cut(s)	444
PcsI	WCGNNNNNNNCGW	5 cut(s)	98, 125, 152, 161, 441
PctI	GAATGC	1 cut(s)	137
PdmI	GAANNNNTTC	1 cut(s)	693
PflFI	GACNNNGTC	3 cut(s)	67, 94, 121
PfoI	TCCNGGA	1 cut(s)	1036
Ple19I	CGATCG	1 cut(s)	442
PleI	GAGTC	3 cut(s)	391, 1071, 1110
PluTI	GGCGCC	1 cut(s)	823
PpsI	GAGTC	3 cut(s)	391, 1071, 1110
PsiI	TTATAA	1 cut(s)	1160
Psp124BI	GAGCTC	1 cut(s)	13
Psp6I	CCWGG	2 cut(s)	481, 1036
PspFI	CCCAGC	2 cut(s)	52, 79
PspGI	CCWGG	2 cut(s)	481, 1036
PspN4I	GGNNCC	4 cut(s)	238, 345, 408, 821
PstI	CTGCAG	1 cut(s)	922
PstNI	CAGNNNCTG	1 cut(s)	707
PsuI	RGATCY	1 cut(s)	658
PsyI	GACNNNGTC	3 cut(s)	67, 94, 121
PvuI	CGATCG	1 cut(s)	442
PvuII	CAGCTG	1 cut(s)	934
RruI	TCGCGA	1 cut(s)	444
RsaI	GTAC	2 cut(s)	613, 969
RsaNI	GTAC	2 cut(s)	612, 968
SacI	GAGCTC	1 cut(s)	13
SalI	GTCGAC	3 cut(s)	100, 127, 202
SaqAI	TTAA	4 cut(s)	504, 654, 881, 980
Sau3AI	GATC	6 cut(s)	439, 529, 658, 780, 941, 1040
ScaI	AGTACT	1 cut(s)	613
SchI	GAGTC	3 cut(s)	391, 1072, 1110
ScrFI	CCNGG	5 cut(s)	107, 134, 185, 483, 1038
SduI	GDGCHC	1 cut(s)	13
SfaNI	GCATC	3 cut(s)	454, 694, 732
SfcI	CTRYAG	1 cut(s)	918
SfoI	GGCGCC	1 cut(s)	821
SgrAI	CRCCGGYG	1 cut(s)	221
SgrDI	CGTCGACG	1 cut(s)	202
Sse9I	AATT	5 cut(s)	382, 389, 456, 899, 1003
SsiI	CCGC	6 cut(s)	219, 234, 327, 359, 533, 1017
SspDI	GGCGCC	1 cut(s)	819
SspMI	CTAG	2 cut(s)	854, 960
SstI	GAGCTC	1 cut(s)	13
StyD4I	CCNGG	5 cut(s)	105, 132, 183, 481, 1036
StyI	CCWWGG	1 cut(s)	776
TaiI	ACGT	1 cut(s)	209
TaqI	TCGA	6 cut(s)	101, 128, 155, 179, 203, 783
TasI	AATT	5 cut(s)	382, 389, 456, 899, 1003
TatI	WGTACW	1 cut(s)	611
TauI	GCSGC	3 cut(s)	237, 330, 361
Tru1I	TTAA	4 cut(s)	504, 654, 881, 980
Tru9I	TTAA	4 cut(s)	504, 654, 881, 980
TseI	GCWGC	8 cut(s)	274, 289, 310, 361, 364, 917, 1070, 1093
TspDTI	ATGAA	2 cut(s)	702, 899
TspGWI	ACGGA	4 cut(s)	189, 243, 336, 1115
Tth111I	GACNNNGTC	3 cut(s)	67, 94, 121
XapI	RAATTY	1 cut(s)	1003
XmiI	GTMKAC	3 cut(s)	101, 128, 203
XmnI	GAANNNNTTC	1 cut(s)	693
XspI	CTAG	2 cut(s)	854, 960
ZraI	GACGTC	1 cut(s)	207
ZrmI	AGTACT	1 cut(s)	613

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.