RchiOBHm_Chr4g0430621

Nuclear pore complex protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0016608
Physical Location & Seq
Forward (+)
55109658 .. 55113332
3675 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39934

Sequence Viewer

Length: 1218 bp
ATGTTCGGAGCTCAATCGTCGTCTTCTCCCTTCGGAGCTCAGACGTCCTCTTCTCCCTTCGGAGCCTTCGGCACTCCATCTTCAACCTTCGGCACTCCGGCTTCGACCTCCGCCTTCGGGACTCCATCCTCGACTCCAGCCTTCGGCACTCCCTCGACGACCCCGTCCTTCTTCACTCCTAGTTCCACCCCCGCCTTCGGAACTCCCACCACGTCGTCGTTTTCCACCGGCGGCTTCGGGACCTCCTTGTTCTCCAGCGCCTTCTCTACTCAGACTCAGCAGCAACAGCAAACGCCGTCGTTTCAGCAACAAGCTCCCTCCGCCGGTGGATTTGGGACTCAGAATCCGTTCGGTACGCCGCAGCAGCAGCAGCAGACGCCGTCGTTTCAGAGTCCCTTTCCTACTCAATTGACCACTCAGATGGCTCCCGTGGCTCCTCTCCCTTACTCTCTCGCCGATCGTGACATTCAGGCAATTGTTGACGCTTATAAGGATGAGCCTGGTAACCCTAAATATGCTTTTAAGCATCTGTTGTTTAGTGTAACTGATCCGCAGTTTAGGTTGAAGCCTGCTGGTGTATCGGATATAATGTGGGCAGAGGCTATGGGGAAGCTTGAAGGTCTGTCAAGTACTGACAGAGAACGGCTGTGGCCTGAGCTTGTTCAGGGTTTTAAGGATCTTTCACAACGCCTGAAGCTCCAAGATGAAGTCATACTTTCAGATGCTGAGAGACTGCAAATGACCCAAAGCAACGTGAAGATGCTTCAAAGACATTTTCAAGCTGACACTATTCCTCGGATTGAAAGAATGAGACAGAAAGAGCAAGGTCTTGAAAGGCGCCTGTTGAGGGTAATGAGAATACTGGAGGCACTGGAGGGCAAAGGTTTCCGATTGCCTTTAATGAAAGGGGAAGCTGAGTTGGCTGAGAAGTTAGCTTCAATAACTAGACAGTTGAAGGGACCTGGAGCTGAGCTTTCTAGCAGGGTACAAAACCTGCGAACTATATCCCGAGTCCAGGCAAATTCTATTGGTGCTGGTGGTTTTGTTTCTCTTCCAGGATCAACCAAAATACATGAACAGAGTCTTGCTGATATGCAAGAGGTCTTACAACAACAGACTGAGGCTATTGCAAGACTAGGCAATGTTTTGAAGCGTGATATTAGGGATATGGAGATTATAATGGCTGAGGAGCCAGACACTACAGATAATATGTGTTAG

Protein Analysis

405

Amino Acids

44.12

Weight (kDa)

6.37

Isoelectric Point (pI)

48.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nup54 PF13874 189 - 333 2.8e-32 Nucleoporin complex subunit 54
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016608)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G24310
fragaria_vesca FvH4_4g23970
malus_domestica MD13G1116800.v1.1 MD16G1117500.v1.1
prunus_persica Prupe.1G228700_v2.0.a1
pyrus_communis pycom13g10140 pycom16g10070
rosa_chinensis RchiOBHm_Chr4g0430621
rosa_laevigata RLG00000006962
rosa_multiflora Rmu_sc0000131.1_g000016
rosa_roxburghii Rroxscaffold_5G00372370
rosa_rugosa Rorug04G0244800
rosa_samantha Rh4DG305500
rosa_wichuraiana Rw4G026100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 489, 1178
AasI GACNNNNNNGTC 1 cut(s) 163
AatII GACGTC 1 cut(s) 47
Acc36I ACCTGC 1 cut(s) 1002
AccB1I GGYRCC 1 cut(s) 837
AciI CCGC 6 cut(s) 111, 192, 231, 321, 359, 551
AclWI GGATC 3 cut(s) 542, 684, 1066
AcsI RAATTY 1 cut(s) 1021
AcuI CTGAAG 1 cut(s) 713
AcyI GRCGYC 3 cut(s) 44, 377, 838
AfaI GTAC 3 cut(s) 355, 631, 987
AfiI CCNNNNNNNGG 6 cut(s) 117, 143, 197, 323, 847, 1015
AjiI CACGTC 1 cut(s) 213
AjnI CCWGG 4 cut(s) 499, 961, 1014, 1054
Alw21I GWGCWC 2 cut(s) 13, 40
Alw26I GTCTC 2 cut(s) 724, 805
AlwI GGATC 3 cut(s) 542, 684, 1066
AlwNI CAGNNNCTG 1 cut(s) 725
Ama87I CYCGRG 1 cut(s) 1008
AoxI GGCC 1 cut(s) 650
ApeKI GCWGC 5 cut(s) 280, 361, 364, 367, 370
ApoI RAATTY 1 cut(s) 1021
Asp700I GAANNNNTTC 1 cut(s) 347
AspLEI GCGC 2 cut(s) 260, 840
AspS9I GGNCC 2 cut(s) 240, 959
AvaI CYCGRG 1 cut(s) 1008
AvaII GGWCC 2 cut(s) 240, 959
BanI GGYRCC 1 cut(s) 837
BanII GRGCYC 2 cut(s) 13, 40
BbsI GAAGAC 1 cut(s) 15
Bbv12I GWGCWC 2 cut(s) 13, 40
BbvCI CCTCAGC 1 cut(s) 1185
BbvI GCAGC 5 cut(s) 292, 373, 376, 379, 382
BccI CCATC 3 cut(s) 85, 133, 415
BceAI ACGGC 3 cut(s) 280, 364, 659
BciT130I CCWGG 4 cut(s) 501, 963, 1016, 1056
BcoDI GTCTC 2 cut(s) 724, 805
BfaI CTAG 4 cut(s) 180, 945, 978, 1136
BfmI CTRYAG 1 cut(s) 1200
BfoI RGCGCY 2 cut(s) 261, 841
BfuAI ACCTGC 1 cut(s) 1002
BisI GCNGC 7 cut(s) 232, 281, 359, 362, 365, 368, 371
BlpI GCTNAGC 1 cut(s) 969
BlsI GCNGC 7 cut(s) 233, 282, 360, 363, 366, 369, 372
BmcAI AGTACT 1 cut(s) 631
Bme1390I CCNGG 4 cut(s) 501, 963, 1016, 1056
Bme18I GGWCC 2 cut(s) 240, 959
BmeT110I CYCGRG 1 cut(s) 1008
BmgBI CACGTC 1 cut(s) 213
BmgT120I GGNCC 2 cut(s) 240, 959
BmiI GGNNCC 7 cut(s) 64, 241, 426, 435, 839, 960, 1191
BmrFI CCNGG 4 cut(s) 501, 963, 1016, 1056
BmsI GCATC 3 cut(s) 535, 712, 750
BpiI GAAGAC 1 cut(s) 15
BpmI CTGGAG 5 cut(s) 120, 238, 884, 893, 984
Bpu10I CCTNAGC 2 cut(s) 654, 1185
Bpu1102I GCTNAGC 1 cut(s) 969
BsaHI GRCGYC 3 cut(s) 44, 377, 838
BsaJI CCNNGG 2 cut(s) 429, 794
Bsc4I CCNNNNNNNGG 6 cut(s) 117, 143, 197, 323, 847, 1015
Bse118I RCCGGY 2 cut(s) 227, 323
Bse1I ACTGG 2 cut(s) 867, 876
Bse3DI GCAATG 1 cut(s) 1147
BseBI CCWGG 4 cut(s) 501, 963, 1016, 1056
BseDI CCNNGG 2 cut(s) 429, 794
BseGI GGATG 2 cut(s) 125, 499
BseLI CCNNNNNNNGG 6 cut(s) 117, 143, 197, 323, 847, 1015
BseMI GCAATG 1 cut(s) 1147
BseNI ACTGG 2 cut(s) 867, 876
BseRI GAGGAG 2 cut(s) 426, 1202
BseXI GCAGC 5 cut(s) 292, 373, 376, 379, 382
Bsh1285I CGRYCG 1 cut(s) 460
BshFI GGCC 1 cut(s) 652
BshNI GGYRCC 1 cut(s) 837
BsiEI CGRYCG 1 cut(s) 460
BsiHKAI GWGCWC 2 cut(s) 13, 40
BsiHKCI CYCGRG 1 cut(s) 1008
BsiSI CCGG 3 cut(s) 98, 228, 324
BslFI GGGAC 5 cut(s) 133, 253, 349, 378, 972
BslI CCNNNNNNNGG 6 cut(s) 117, 143, 197, 323, 847, 1015
BsmAI GTCTC 2 cut(s) 724, 805
BsmFI GGGAC 5 cut(s) 133, 253, 349, 378, 972
BsnI GGCC 1 cut(s) 652
BsoBI CYCGRG 1 cut(s) 1008
Bsp1286I GDGCHC 2 cut(s) 13, 40
Bsp143I GATC 4 cut(s) 457, 547, 676, 1058
Bsp1720I GCTNAGC 1 cut(s) 969
BspACI CCGC 6 cut(s) 111, 192, 231, 321, 359, 551
BspANI GGCC 1 cut(s) 652
BspLI GGNNCC 7 cut(s) 64, 241, 426, 435, 839, 960, 1191
BspMI ACCTGC 1 cut(s) 1002
BspPI GGATC 3 cut(s) 542, 684, 1066
BspT107I GGYRCC 1 cut(s) 837
BsrDI GCAATG 1 cut(s) 1147
BsrFI RCCGGY 2 cut(s) 227, 323
BsrI ACTGG 2 cut(s) 867, 876
BssAI RCCGGY 2 cut(s) 227, 323
BssECI CCNNGG 2 cut(s) 429, 794
BssMI GATC 4 cut(s) 457, 547, 676, 1058
BssNI GRCGYC 3 cut(s) 44, 377, 838
Bst2UI CCWGG 4 cut(s) 501, 963, 1016, 1056
Bst4CI ACNGT 1 cut(s) 951
Bst6I CTCTTC 2 cut(s) 55, 1056
BstACI GRCGYC 3 cut(s) 44, 377, 838
BstC8I GCNNGC 1 cut(s) 570
BstDSI CCRYGG 1 cut(s) 429
BstEII GGTNACC 1 cut(s) 503
BstF5I GGATG 2 cut(s) 125, 499
BstH2I RGCGCY 2 cut(s) 261, 841
BstHHI GCGC 2 cut(s) 260, 840
BstKTI GATC 4 cut(s) 460, 550, 679, 1061
BstMAI GTCTC 2 cut(s) 724, 805
BstMBI GATC 4 cut(s) 457, 547, 676, 1058
BstMCI CGRYCG 1 cut(s) 460
BstMWI GCNNNNNNNGC 8 cut(s) 286, 320, 364, 367, 370, 376, 431, 920
BstNI CCWGG 4 cut(s) 501, 963, 1016, 1056
BstPI GGTNACC 1 cut(s) 503
BstSCI CCNGG 4 cut(s) 499, 961, 1014, 1054
BstSFI CTRYAG 1 cut(s) 1200
BstV1I GCAGC 5 cut(s) 292, 373, 376, 379, 382
BstV2I GAAGAC 1 cut(s) 15
BstX2I RGATCY 1 cut(s) 676
BstXI CCANNNNNNTGG 1 cut(s) 421
BstYI RGATCY 1 cut(s) 676
BsuRI GGCC 1 cut(s) 652
BtgI CCRYGG 1 cut(s) 429
BtrI CACGTC 1 cut(s) 213
BtsCI GGATG 2 cut(s) 125, 499
BtsIMutI CAGTG 1 cut(s) 869
BveI ACCTGC 1 cut(s) 1002
Cac8I GCNNGC 1 cut(s) 570
CaiI CAGNNNCTG 1 cut(s) 725
CfoI GCGC 2 cut(s) 260, 840
Cfr10I RCCGGY 2 cut(s) 227, 323
Cfr13I GGNCC 2 cut(s) 240, 959
CseI GACGC 2 cut(s) 385, 491
Csp6I GTAC 3 cut(s) 354, 630, 986
CviAII CATG 1 cut(s) 1073
CviQI GTAC 3 cut(s) 354, 630, 986
DinI GGCGCC 1 cut(s) 839
DpnI GATC 4 cut(s) 459, 549, 678, 1060
DpnII GATC 4 cut(s) 457, 547, 676, 1058
DrdI GACNNNNNNGTC 1 cut(s) 163
DseDI GACNNNNNNGTC 1 cut(s) 163
Eam1104I CTCTTC 2 cut(s) 55, 1056
EarI CTCTTC 2 cut(s) 55, 1056
EciI GGCGGA 2 cut(s) 100, 310
Ecl136II GAGCTC 2 cut(s) 11, 38
Eco24I GRGCYC 2 cut(s) 13, 40
Eco47I GGWCC 2 cut(s) 240, 959
Eco53kI GAGCTC 2 cut(s) 11, 38
Eco57I CTGAAG 1 cut(s) 713
Eco88I CYCGRG 1 cut(s) 1008
Eco91I GGTNACC 1 cut(s) 503
EcoICRI GAGCTC 2 cut(s) 11, 38
EcoO109I RGGNCCY 2 cut(s) 240, 959
EcoO65I GGTNACC 1 cut(s) 503
EcoRII CCWGG 4 cut(s) 499, 961, 1014, 1054
EcoT38I GRGCYC 2 cut(s) 13, 40
EgeI GGCGCC 1 cut(s) 839
EheI GGCGCC 1 cut(s) 839
FaeI CATG 1 cut(s) 1076
FalI AAGNNNNNCTT 2 cut(s) 699, 731
FaqI GGGAC 5 cut(s) 133, 253, 349, 378, 972
FatI CATG 1 cut(s) 1072
FauI CCCGC 1 cut(s) 199
Fnu4HI GCNGC 7 cut(s) 232, 281, 359, 362, 365, 368, 371
FokI GGATG 2 cut(s) 112, 506
FriOI GRGCYC 2 cut(s) 13, 40
Fsp4HI GCNGC 7 cut(s) 232, 281, 359, 362, 365, 368, 371
FspBI CTAG 4 cut(s) 180, 945, 978, 1136
GlaI GCGC 2 cut(s) 259, 839
GluI GCNGC 7 cut(s) 232, 281, 359, 362, 365, 368, 371
GsuI CTGGAG 5 cut(s) 120, 238, 884, 893, 984
HaeII RGCGCY 2 cut(s) 261, 841
HaeIII GGCC 1 cut(s) 652
HapII CCGG 3 cut(s) 98, 228, 324
HgaI GACGC 2 cut(s) 385, 491
HhaI GCGC 2 cut(s) 260, 840
Hin1I GRCGYC 3 cut(s) 44, 377, 838
Hin1II CATG 1 cut(s) 1076
Hin6I GCGC 2 cut(s) 258, 838
HinP1I GCGC 2 cut(s) 258, 838
HincII GTYRAC 1 cut(s) 481
HindII GTYRAC 1 cut(s) 481
HindIII AAGCTT 1 cut(s) 611
HinfI GANTC 8 cut(s) 121, 133, 274, 337, 343, 391, 1011, 1081
HpaII CCGG 3 cut(s) 98, 228, 324
Hpy166II GTNNAC 1 cut(s) 481
Hpy188III TCNNGA 5 cut(s) 118, 238, 461, 830, 1008
Hpy8I GTNNAC 1 cut(s) 481
Hpy99I CGWCG 6 cut(s) 22, 160, 217, 220, 301, 385
HpyCH4III ACNGT 1 cut(s) 951
HpyCH4IV ACGT 3 cut(s) 44, 212, 753
HpyCH4V TGCA 3 cut(s) 736, 1096, 1130
HpyF10VI GCNNNNNNNGC 8 cut(s) 286, 320, 364, 367, 370, 376, 431, 920
HpySE526I ACGT 3 cut(s) 44, 212, 753
Hsp92I GRCGYC 3 cut(s) 44, 377, 838
Hsp92II CATG 1 cut(s) 1076
HspAI GCGC 2 cut(s) 258, 838
KasI GGCGCC 1 cut(s) 837
Kzo9I GATC 4 cut(s) 457, 547, 676, 1058
LmnI GCTCC 9 cut(s) 8, 35, 62, 319, 430, 439, 702, 965, 1189
Lsp1109I GCAGC 5 cut(s) 292, 373, 376, 379, 382
LweI GCATC 3 cut(s) 535, 712, 750
MaeI CTAG 4 cut(s) 180, 945, 978, 1136
MaeII ACGT 3 cut(s) 44, 212, 753
MaeIII GTNAC 3 cut(s) 461, 503, 541
MalI GATC 4 cut(s) 459, 549, 678, 1060
MboI GATC 4 cut(s) 457, 547, 676, 1058
MboII GAAGA 6 cut(s) 15, 42, 72, 163, 769, 1043
MfeI CAATTG 2 cut(s) 407, 474
MflI RGATCY 1 cut(s) 676
MhlI GDGCHC 2 cut(s) 13, 40
MluCI AATT 3 cut(s) 407, 474, 1021
Mly113I GGCGCC 1 cut(s) 838
MlyI GAGTC 7 cut(s) 115, 127, 268, 331, 400, 1020, 1090
MroXI GAANNNNTTC 1 cut(s) 347
MseI TTAA 3 cut(s) 522, 672, 899
MslI CAYNNNNRTG 1 cut(s) 419
MspI CCGG 3 cut(s) 98, 228, 324
MspR9I CCNGG 4 cut(s) 501, 963, 1016, 1056
MunI CAATTG 2 cut(s) 407, 474
MvaI CCWGG 4 cut(s) 501, 963, 1016, 1056
MwoI GCNNNNNNNGC 8 cut(s) 286, 320, 364, 367, 370, 376, 431, 920
NarI GGCGCC 1 cut(s) 838
NdeII GATC 4 cut(s) 457, 547, 676, 1058
NlaIII CATG 1 cut(s) 1076
NlaIV GGNNCC 7 cut(s) 64, 241, 426, 435, 839, 960, 1191
NmuCI GTSAC 1 cut(s) 461
PcsI WCGNNNNNNNCGW 1 cut(s) 161
PdmI GAANNNNTTC 1 cut(s) 347
PfeI GAWTC 1 cut(s) 343
PflFI GACNNNGTC 2 cut(s) 163, 379
PfoI TCCNGGA 1 cut(s) 1054
PkrI GCNGC 7 cut(s) 233, 282, 360, 363, 366, 369, 372
Ple19I CGATCG 1 cut(s) 460
PleI GAGTC 7 cut(s) 115, 127, 268, 331, 399, 1019, 1089
PluTI GGCGCC 1 cut(s) 841
PpsI GAGTC 7 cut(s) 115, 127, 268, 331, 399, 1019, 1089
PpuMI RGGWCCY 2 cut(s) 240, 959
PsiI TTATAA 2 cut(s) 489, 1178
Psp124BI GAGCTC 2 cut(s) 13, 40
Psp5II RGGWCCY 2 cut(s) 240, 959
Psp6I CCWGG 4 cut(s) 499, 961, 1014, 1054
PspEI GGTNACC 1 cut(s) 503
PspGI CCWGG 4 cut(s) 499, 961, 1014, 1054
PspN4I GGNNCC 7 cut(s) 64, 241, 426, 435, 839, 960, 1191
PspPI GGNCC 2 cut(s) 240, 959
PspPPI RGGWCCY 2 cut(s) 240, 959
PstNI CAGNNNCTG 1 cut(s) 725
PsuI RGATCY 1 cut(s) 676
PsyI GACNNNGTC 2 cut(s) 163, 379
PvuI CGATCG 1 cut(s) 460
RsaI GTAC 3 cut(s) 355, 631, 987
RsaNI GTAC 3 cut(s) 354, 630, 986
RseI CAYNNNNRTG 1 cut(s) 419
SacI GAGCTC 2 cut(s) 13, 40
SaqAI TTAA 3 cut(s) 522, 672, 899
SatI GCNGC 7 cut(s) 232, 281, 359, 362, 365, 368, 371
Sau3AI GATC 4 cut(s) 457, 547, 676, 1058
Sau96I GGNCC 2 cut(s) 240, 959
ScaI AGTACT 1 cut(s) 631
SchI GAGTC 7 cut(s) 115, 127, 268, 331, 400, 1020, 1090
ScrFI CCNGG 4 cut(s) 501, 963, 1016, 1056
SduI GDGCHC 2 cut(s) 13, 40
SfaNI GCATC 3 cut(s) 535, 712, 750
SfcI CTRYAG 1 cut(s) 1200
SfoI GGCGCC 1 cut(s) 839
SgrAI CRCCGGYG 2 cut(s) 227, 323
SinI GGWCC 2 cut(s) 240, 959
SmiMI CAYNNNNRTG 1 cut(s) 419
Sse9I AATT 3 cut(s) 407, 474, 1021
SsiI CCGC 6 cut(s) 111, 192, 231, 321, 359, 551
SspDI GGCGCC 1 cut(s) 837
SspMI CTAG 4 cut(s) 180, 945, 978, 1136
SstI GAGCTC 2 cut(s) 13, 40
StyD4I CCNGG 4 cut(s) 499, 961, 1014, 1054
TaaI ACNGT 1 cut(s) 951
TaiI ACGT 3 cut(s) 47, 215, 756
TaqI TCGA 3 cut(s) 104, 131, 155
TasI AATT 3 cut(s) 407, 474, 1021
TatI WGTACW 1 cut(s) 629
TauI GCSGC 2 cut(s) 234, 361
TfiI GAWTC 1 cut(s) 343
Tru1I TTAA 3 cut(s) 522, 672, 899
Tru9I TTAA 3 cut(s) 522, 672, 899
TscAI CASTG 1 cut(s) 876
TseFI GTSAC 1 cut(s) 461
TseI GCWGC 5 cut(s) 280, 361, 364, 367, 370
Tsp45I GTSAC 1 cut(s) 461
TspDTI ATGAA 3 cut(s) 720, 917, 1089
TspGWI ACGGA 1 cut(s) 336
TspRI CASTG 1 cut(s) 876
Tth111I GACNNNGTC 2 cut(s) 163, 379
VpaK11BI GGWCC 2 cut(s) 240, 959
XapI RAATTY 1 cut(s) 1021
XmnI GAANNNNTTC 1 cut(s) 347
XspI CTAG 4 cut(s) 180, 945, 978, 1136
ZraI GACGTC 1 cut(s) 45
ZrmI AGTACT 1 cut(s) 631
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.