Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0005119

Physical Location & Seq

Genomic Coordinates Reverse (-)

6866653 .. 6873865

Gene Structure 7213 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2955 bp

ATGGTTGGATCCGGAGGGGATCCGTCCCCACTTTCCGACTTCCTTCACCTCTCCGAACCAAAAGCCACCACAACGATCACAAGTTCCCAACGTCCCGTATCTGGGGAAGGTAGTTTTTTGCGTACGCACTACCCCAACTCTCCGACTTCCTTCACCTCTCCGAACCAAAAGCCACCGCAACGATCACAAGTTCCCAACGTCCCGTATCTGGGGAAGGTAGTTCTTTGCGTACGCACTTCCCCTCTCTCCGACTTCCTTCACCTCTCCGATTCAAAAGCCACCATTTCCAATCGTCTCTTCACAAATCCGCACACCCACCAACTGCATAGAGATCGGGAAAAAATGGCGTTGGGATTTAGCTCCGGCGACGCGCCTGGCTTCAAGTTGCTGCTCATTCTGGTAGCGACGTACGGCCTCATGGCGGCGCTGGTTCACTCCGTACTGTACATGCGCTTCATCAAGCCGCTCGACATCGACGCCCCTCTCGATCGCTTCTCCGAAGCCAGAGCCGTCGAGCACGTTCGAGTGTTGGCCCATGAGATCGACGGCCGCCAGGAAGGGCGTCCTGGCTTGACCGAAGCTGCTCGGTACATTACAGGGCAGTTGGAAATGATAAAGGAGCGAGCTGGGTCCAATGTTAGAATTGAGATAGAAGAGTCCGTTGTTAATGGCACCTTTAATATGATGTTTTTGGGCCATGGCATTTCTCTTGGTTACAGAAACCACACAAATATTGTGATGAGGATATCGTCAATACATTCACAAGACAATGAACCATCAGTTTTGTTAAATGGACATTTTGACAGTCCACTTGCTTCCCCAGGTGCTGCTGATTGCGGTTCATGTGTAGCATCAATGCTGGAAATAGCAAGGCTAATGGTGGATTCTGGATGGGTTCCTCCTCGGCCTGTTATTTTTCTTTTTAATGGGGCTGAAGAGCTTTTCTTGTTGGGTTCTCATGGCTTCATGAAGACACATCAATGGCGTGAGACTATTGGGGCTTTTATAAATGTGGAGGCATCAGGGACCCGCGGTCCTGATTTAGTTTGTCAATCAGGACCTGGTTCTTGGCCCTCTCAGGTCTATGCTCAATCAGCCGTATATCCTATGGCACATAGCGCAGCTCAGGATGTTTTTCCTGTCATTCCTGGAGATACAGATTTCCGTATATTTTCTCAAGATTATGGCAACATTCCAGGCTTGGATATTATCTTTCTCCTTGGTGGTTATTTTTATCATACATCCTATGATACAATGGAGAGATTATTACCTGGAAGTATGCAAGCTCGGGGAGAGAATTTGGTTAGCGTAATTAAGGCCTTCACCAATTCTTCTAAGCTACAAGTTAAACATGAAAGAGAGTCGAATGCATATCACTATGACGGAGAACGTGCTGTTTTCTTTGATTATTTATCATTGTTTATGATTTACTATACGCGGAAGGTAGCCAGGCTACTTCACAGTATTCCTATTGCCATTTTCTTAGCTATGCCATTTTTTTCACACAAGCAGAATCGTGGGCTTGTTTCTTGGTTTTCAACTTTCTGCGATTTTACGAAAGGAATGATTTTCCATGCTACTGGGATTTTTCTGGCAATTGTATTTCCAATTATCTTTGCCATCCTGAGATTGCTGTTCACTAGTTGTGCTATGAATTGGTTTGCTCATCCATATTTGGCTTACTTGATGTTCGTCCCCTGCTCACTTGTTGGTATGTTGATTCCTAGAATTATTTGGAGTAGCTTTCCCCTCTCTCAAGATGCATCAGCTCTCAAGTCTTTAAAAGAGGCTTTATCTGATGAAGCGAGGTTTTGGGGAGCATTTGGATTATACGCTATATCAACTTTGGCTTACCTTTTGGCTGGGCTTAGCGGAGGCTTCTTGACTTTTTCAATATCTACCTCTATGCTTCCTGGATGGATTTCATACTGCTTATCAGTCAAATTATTTGGTCACCAGTCACTCAGGTCAACATTATTTTACGTGTTACCTGTACTTCCATGCCTTGCATACTCTGTATATTTTGGTGGATTTCTTGTACAATTTGTGGTTGAAAAGCTGGGTATGATGGGTGCTTTGCCACCTCCATATGGTTATTTTGTTCCTGATGTTGTGATGGCAACAACAGTGGGAGCTGTGACTGGTTTGTGTGTAGGCCCTCTGATACCAATCTGTGGTCGCTGGTTAGCTAGGTCATCCATTTTGCAGGTTTTGCTGCACATTAGTGTGCTAGGCTTGGCTTTGTCGTCTCAGTTCTTTCCATACAGTGTGGACGCTCCTAAGCGGATTCTTTTCCAGCATACGTTTCTTACTGCAGATGCAAATCAGGTTATGGACTCCAGCTATGATTTTTCTGTTGTAGATTCCAATTCTTTACTTTTTGTATTCAAACATGCACCGGAGGTGGCAAAGGAATTGCACATTAGTTCAGAGTTATCTTTTGAAACTGCAAAGTTATCTCAGCCGGAAAATTTTATGGGAATTTTTCCTGTGTCTTTTTTGTTCTCAAGAAGCTTGAAGTTCCCTGCAAAAAGTGACAGTATTTTGAAGCAATACAGGCAGTTCCCCCATCTGTCTACTTATGAGCCGCATACGGTTTTCATTGAGGGATCTCGCAGAGTATACTTGCAACTGTCTTTAGGTTCCTTAAAGGAGGTTTGGGTTACCGTCCTCAACATAACAGGCCCTTTATCCAGTTGGTCATTTGCAGACAATACACTTCCAGCTACTGTAACGAATGACGGAGGTCCACCATCATACATATGCCGGCTTAGTGGAGCTAGCTCTGAGAATTGGAACTTCTGGTTAGAGGCTAGGGGATCCGACGGTTTGAGGGTTGACATAGCTGTCATAGACCAATATATGGTTGACGAAACAAAGAAGCTGAGAGGCCTTTTTCCAGAGTGGGTGGATGTCGTTGCTTATTCGAGCTTCTTGTCAAGTTATATCTTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001541 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0007275 GO:0007548 GO:0008150 GO:0008406 GO:0008585 GO:0012505 GO:0022414 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045137 GO:0046545 GO:0046660 GO:0048513 GO:0048608 GO:0048731 GO:0048856 GO:0061458

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_M28

Protein Analysis

985

Amino Acids

109.16

Weight (kDa)

6.67

Isoelectric Point (pI)

39.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M28	PF04389	244 - 436	5.2e-45	Peptidase family M28
ERMP1_C	PF22248	759 - 982	3.3e-32	Endoplasmic reticulum metallopeptidase 1, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0005119)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G67420 AT1G67420 AT1G67420 AT1G67420 AT1G67420 AT1G67420
fragaria_vesca	FvH4_4g23910 FvH4_4g23910 FvH4_4g23910 FvH4_4g23910 FvH4_4g23910 FvH4_4g23910
malus_domestica	MD13G1117700.v1.1 MD16G1117900.v1.1
prunus_persica	Prupe.1G227300_v2.0.a1 Prupe.1G227300_v2.0.a1 Prupe.1G227300_v2.0.a1
pyrus_communis	pycom13g10180 pycom16g10100
rosa_chinensis	RchiOBHm_Chr4g0430551
rosa_laevigata	RLG00000006970
rosa_multiflora	Rmu_sc0011782.1_g000005
rosa_roxburghii	Rroxscaffold_5G00372320
rosa_rugosa	Rorug04G0244100 Rorug04G0244200
rosa_samantha	Rh4AG300200 Rh4BG307300 Rh4CG324000 Rh4DG304600
rosa_wichuraiana	Rw4G026050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1007
AasI	GACNNNNNNGTC	1 cut(s)	2846
Acc36I	ACCTGC	1 cut(s)	2197
AccB1I	GGYRCC	1 cut(s)	671
AccB7I	CCANNNNNTGG	2 cut(s)	2174, 2863
AccBSI	CCGCTC	1 cut(s)	466
AccI	GTMKAC	2 cut(s)	2576, 2622
AccII	CGCG	3 cut(s)	371, 1032, 1438
AccIII	TCCGGA	1 cut(s)	11
AclWI	GGATC	7 cut(s)	3, 14, 16, 27, 2617, 2814, 2827
AcoI	YGGCCR	1 cut(s)	547
AcsI	RAATTY	3 cut(s)	1297, 2470, 2481
AcuI	CTGAAG	1 cut(s)	954
AcyI	GRCGYC	2 cut(s)	477, 562
AfaI	GTAC	8 cut(s)	124, 231, 410, 441, 446, 590, 1995, 2040
AfiI	CCNNNNNNNGG	8 cut(s)	101, 102, 208, 209, 421, 2090, 2174, 2863
AflIII	ACRYGT	1 cut(s)	1983
AgsI	TTSAA	9 cut(s)	273, 382, 1539, 1893, 2054, 2389, 2444, 2518, 2548
AhdI	GACNNNNNGTC	1 cut(s)	1032
AhlI	ACTAGT	1 cut(s)	1640
AjuI	GAANNNNNNNTTGG	2 cut(s)	281, 313
AleI	CACNNNNGTG	1 cut(s)	2223
Alw21I	GWGCWC	1 cut(s)	519
Alw26I	GTCTC	3 cut(s)	299, 983, 2253
AlwI	GGATC	7 cut(s)	3, 14, 16, 27, 2617, 2814, 2827
AlwNI	CAGNNNCTG	3 cut(s)	827, 1061, 2729
Ama87I	CYCGRG	1 cut(s)	1287
Aor13HI	TCCGGA	1 cut(s)	11
ApeKI	GCWGC	5 cut(s)	388, 581, 827, 1121, 2215
ApoI	RAATTY	3 cut(s)	1297, 2470, 2481
AspLEI	GCGC	4 cut(s)	373, 427, 453, 1121
AsuHPI	GGTGA	5 cut(s)	38, 145, 251, 1315, 1946
AsuNHI	GCTAGC	1 cut(s)	2780
AvaI	CYCGRG	1 cut(s)	1287
AvaII	GGWCC	5 cut(s)	630, 1026, 1034, 1058, 2747
BamHI	GGATCC	3 cut(s)	8, 19, 2819
BanI	GGYRCC	1 cut(s)	671
BarI	GAAGNNNNNNTAC	2 cut(s)	437, 469
BbsI	GAAGAC	1 cut(s)	977
Bbv12I	GWGCWC	1 cut(s)	519
BbvI	GCAGC	5 cut(s)	375, 568, 814, 1133, 2202
BccI	CCATC	8 cut(s)	784, 885, 1628, 1911, 2062, 2110, 2577, 2761
BceAI	ACGGC	4 cut(s)	427, 494, 562, 1082
BcgI	CGANNNNNNTGC	2 cut(s)	314, 348
BcoDI	GTCTC	3 cut(s)	299, 983, 2253
BcuI	ACTAGT	1 cut(s)	1640
BfaI	CTAG	7 cut(s)	1641, 1725, 2190, 2231, 2781, 2814, 2953
BfmI	CTRYAG	1 cut(s)	2313
BfoI	RGCGCY	1 cut(s)	428
BfuAI	ACCTGC	1 cut(s)	2197
BisI	GCNGC	9 cut(s)	389, 423, 464, 550, 582, 828, 1122, 2216, 2588
BlpI	GCTNAGC	1 cut(s)	1868
BlsI	GCNGC	9 cut(s)	390, 424, 465, 551, 583, 829, 1123, 2217, 2589
Bme18I	GGWCC	5 cut(s)	630, 1026, 1034, 1058, 2747
BmeRI	GACNNNNNGTC	1 cut(s)	1032
BmeT110I	CYCGRG	1 cut(s)	1287
BmiI	GGNNCC	9 cut(s)	10, 21, 631, 673, 897, 1027, 1028, 2644, 2821
BmrI	ACTGGG	1 cut(s)	1590
BmsI	GCATC	5 cut(s)	860, 1028, 1750, 1772, 2308
BmtI	GCTAGC	1 cut(s)	2784
BmuI	ACTGGG	1 cut(s)	1590
BoxI	GACNNNNGTC	1 cut(s)	2745
BpiI	GAAGAC	1 cut(s)	977
BpmI	CTGGAG	2 cut(s)	1170, 2323
Bpu10I	CCTNAGC	2 cut(s)	1125, 2280
Bpu1102I	GCTNAGC	1 cut(s)	1868
BpuEI	CTTGAG	4 cut(s)	1161, 1740, 1757, 2491
BsaAI	YACGTR	1 cut(s)	1984
BsaBI	GATNNNNATC	2 cut(s)	2168, 2322
BsaHI	GRCGYC	2 cut(s)	477, 562
BsaJI	CCNNGG	5 cut(s)	697, 820, 902, 1030, 1219
BsaWI	WCCGGW	2 cut(s)	11, 2398
Bsc4I	CCNNNNNNNGG	8 cut(s)	101, 102, 208, 209, 421, 2090, 2174, 2863
Bse118I	RCCGGY	1 cut(s)	2766
Bse1I	ACTGG	4 cut(s)	1585, 1957, 2146, 2694
Bse8I	GATNNNNATC	2 cut(s)	2168, 2322
BseAI	TCCGGA	1 cut(s)	11
BseDI	CCNNGG	5 cut(s)	697, 820, 902, 1030, 1219
BseGI	GGATG	8 cut(s)	896, 1135, 1241, 1620, 1666, 1922, 2195, 2917
BseJI	GATNNNNATC	2 cut(s)	2168, 2322
BseLI	CCNNNNNNNGG	8 cut(s)	101, 102, 208, 209, 421, 2090, 2174, 2863
BseMII	CTCAG	8 cut(s)	1091, 1139, 1616, 1978, 2264, 2474, 2778, 2876
BseNI	ACTGG	4 cut(s)	1585, 1957, 2146, 2694
BseRI	GAGGAG	1 cut(s)	891
BseX3I	CGGCCG	1 cut(s)	547
BseXI	GCAGC	5 cut(s)	375, 568, 814, 1133, 2202
BseYI	CCCAGC	3 cut(s)	626, 1862, 2059
BsgI	GTGCAG	1 cut(s)	2201
Bsh1236I	CGCG	3 cut(s)	371, 1032, 1438
Bsh1285I	CGRYCG	2 cut(s)	490, 550
BshNI	GGYRCC	1 cut(s)	671
BsiEI	CGRYCG	2 cut(s)	490, 550
BsiHKAI	GWGCWC	1 cut(s)	519
BsiHKCI	CYCGRG	1 cut(s)	1287
BsiSI	CCGG	5 cut(s)	12, 363, 2399, 2465, 2767
BsiWI	CGTACG	3 cut(s)	122, 229, 408
BslFI	GGGAC	5 cut(s)	10, 78, 185, 1039, 1679
BslI	CCNNNNNNNGG	8 cut(s)	101, 102, 208, 209, 421, 2090, 2174, 2863
BsmAI	GTCTC	3 cut(s)	299, 983, 2253
BsmBI	CGTCTC	2 cut(s)	299, 2253
BsmFI	GGGAC	5 cut(s)	10, 78, 185, 1039, 1679
BsmI	GAATGC	1 cut(s)	1372
BsoBI	CYCGRG	1 cut(s)	1287
Bsp1286I	GDGCHC	1 cut(s)	519
Bsp13I	TCCGGA	1 cut(s)	11
Bsp1407I	TGTACA	2 cut(s)	444, 2038
Bsp143I	GATC	9 cut(s)	8, 19, 75, 182, 331, 487, 540, 2609, 2819
Bsp1720I	GCTNAGC	1 cut(s)	1868
Bsp19I	CCATGG	1 cut(s)	697
BspCNI	CTCAG	8 cut(s)	1090, 1138, 1617, 1977, 2263, 2473, 2779, 2877
BspEI	TCCGGA	1 cut(s)	11
BspFNI	CGCG	3 cut(s)	371, 1032, 1438
BspHI	TCATGA	1 cut(s)	966
BspLI	GGNNCC	9 cut(s)	10, 21, 631, 673, 897, 1027, 1028, 2644, 2821
BspMAI	CTGCAG	1 cut(s)	2317
BspMI	ACCTGC	1 cut(s)	2197
BspOI	GCTAGC	1 cut(s)	2784
BspPI	GGATC	7 cut(s)	3, 14, 16, 27, 2617, 2814, 2827
BspQI	GCTCTTC	1 cut(s)	930
BspT107I	GGYRCC	1 cut(s)	671
BsrBI	CCGCTC	1 cut(s)	466
BsrFI	RCCGGY	1 cut(s)	2766
BsrGI	TGTACA	2 cut(s)	444, 2038
BsrI	ACTGG	4 cut(s)	1585, 1957, 2146, 2694
BssAI	RCCGGY	1 cut(s)	2766
BssECI	CCNNGG	5 cut(s)	697, 820, 902, 1030, 1219
BssMI	GATC	9 cut(s)	8, 19, 75, 182, 331, 487, 540, 2609, 2819
BssNAI	GTATAC	1 cut(s)	2623
BssNI	GRCGYC	2 cut(s)	477, 562
BssT1I	CCWWGG	2 cut(s)	697, 1219
Bst1107I	GTATAC	1 cut(s)	2623
Bst6I	CTCTTC	3 cut(s)	302, 648, 930
BstACI	GRCGYC	2 cut(s)	477, 562
BstAPI	GCANNNNNTGC	1 cut(s)	2212
BstAUI	TGTACA	2 cut(s)	444, 2038
BstBAI	YACGTR	1 cut(s)	1984
BstC8I	GCNNGC	4 cut(s)	624, 1284, 2768, 2782
BstDSI	CCRYGG	2 cut(s)	697, 1030
BstEII	GGTNACC	2 cut(s)	1952, 2662
BstF5I	GGATG	8 cut(s)	896, 1135, 1241, 1620, 1666, 1922, 2195, 2917
BstFNI	CGCG	3 cut(s)	371, 1032, 1438
BstH2I	RGCGCY	1 cut(s)	428
BstHHI	GCGC	4 cut(s)	373, 427, 453, 1121
BstKTI	GATC	9 cut(s)	11, 22, 78, 185, 334, 490, 543, 2612, 2822
BstMAI	GTCTC	3 cut(s)	299, 983, 2253
BstMBI	GATC	9 cut(s)	8, 19, 75, 182, 331, 487, 540, 2609, 2819
BstMCI	CGRYCG	2 cut(s)	490, 550
BstMWI	GCNNNNNNNGC	4 cut(s)	1094, 1118, 2212, 2557
BstNSI	RCATGY	2 cut(s)	451, 2396
BstPAI	GACNNNNGTC	1 cut(s)	2745
BstPI	GGTNACC	2 cut(s)	1952, 2662
BstSFI	CTRYAG	1 cut(s)	2313
BstUI	CGCG	3 cut(s)	371, 1032, 1438
BstV1I	GCAGC	5 cut(s)	375, 568, 814, 1133, 2202
BstV2I	GAAGAC	1 cut(s)	977
BstX2I	RGATCY	4 cut(s)	8, 19, 2609, 2819
BstXI	CCANNNNNNTGG	1 cut(s)	1580
BstYI	RGATCY	4 cut(s)	8, 19, 2609, 2819
BstZ17I	GTATAC	1 cut(s)	2623
BstZI	CGGCCG	1 cut(s)	547
BtgI	CCRYGG	2 cut(s)	697, 1030
BtsCI	GGATG	8 cut(s)	896, 1135, 1241, 1620, 1666, 1922, 2195, 2917
BtsIMutI	CAGTG	2 cut(s)	2133, 2272
BveI	ACCTGC	1 cut(s)	2197
Cac8I	GCNNGC	4 cut(s)	624, 1284, 2768, 2782
CaiI	CAGNNNCTG	3 cut(s)	827, 1061, 2729
CciI	TCATGA	1 cut(s)	966
CfoI	GCGC	4 cut(s)	373, 427, 453, 1121
Cfr10I	RCCGGY	1 cut(s)	2766
Cfr42I	CCGCGG	1 cut(s)	1033
CseI	GACGC	4 cut(s)	377, 485, 551, 2282
CsiI	ACCWGGT	1 cut(s)	1060
Csp6I	GTAC	8 cut(s)	123, 230, 409, 440, 445, 589, 1994, 2039
CspCI	CAANNNNNGTGG	2 cut(s)	2109, 2144
CviQI	GTAC	8 cut(s)	123, 230, 409, 440, 445, 589, 1994, 2039
DpnI	GATC	9 cut(s)	10, 21, 77, 184, 333, 489, 542, 2611, 2821
DpnII	GATC	9 cut(s)	8, 19, 75, 182, 331, 487, 540, 2609, 2819
DraI	TTTAAA	1 cut(s)	1782
DrdI	GACNNNNNNGTC	1 cut(s)	2846
DriI	GACNNNNNGTC	1 cut(s)	1032
DseDI	GACNNNNNNGTC	1 cut(s)	2846
EaeI	YGGCCR	1 cut(s)	547
EagI	CGGCCG	1 cut(s)	547
Eam1104I	CTCTTC	3 cut(s)	302, 648, 930
Eam1105I	GACNNNNNGTC	1 cut(s)	1032
EarI	CTCTTC	3 cut(s)	302, 648, 930
EclXI	CGGCCG	1 cut(s)	547
Eco130I	CCWWGG	2 cut(s)	697, 1219
Eco147I	AGGCCT	2 cut(s)	1319, 2892
Eco32I	GATATC	1 cut(s)	747
Eco47I	GGWCC	5 cut(s)	630, 1026, 1034, 1058, 2747
Eco52I	CGGCCG	1 cut(s)	547
Eco57I	CTGAAG	1 cut(s)	954
Eco88I	CYCGRG	1 cut(s)	1287
Eco91I	GGTNACC	2 cut(s)	1952, 2662
EcoO109I	RGGNCCY	4 cut(s)	1026, 1058, 2156, 2684
EcoO65I	GGTNACC	2 cut(s)	1952, 2662
EcoRV	GATATC	1 cut(s)	747
EcoT14I	CCWWGG	2 cut(s)	697, 1219
EcoT22I	ATGCAT	2 cut(s)	1372, 1765
ErhI	CCWWGG	2 cut(s)	697, 1219
Esp3I	CGTCTC	2 cut(s)	299, 2253
FaqI	GGGAC	5 cut(s)	10, 78, 185, 1039, 1679
FauI	CCCGC	1 cut(s)	1037
FauNDI	CATATG	2 cut(s)	2089, 2762
FblI	GTMKAC	2 cut(s)	2576, 2622
Fnu4HI	GCNGC	9 cut(s)	389, 423, 464, 550, 582, 828, 1122, 2216, 2588
FokI	GGATG	8 cut(s)	903, 1142, 1228, 1607, 1653, 1929, 2182, 2924
Fsp4HI	GCNGC	9 cut(s)	389, 423, 464, 550, 582, 828, 1122, 2216, 2588
FspBI	CTAG	7 cut(s)	1641, 1725, 2190, 2231, 2781, 2814, 2953
GlaI	GCGC	4 cut(s)	372, 426, 452, 1120
GluI	GCNGC	9 cut(s)	389, 423, 464, 550, 582, 828, 1122, 2216, 2588
GsaI	CCCAGC	3 cut(s)	630, 1866, 2063
GsuI	CTGGAG	2 cut(s)	1170, 2323
HaeII	RGCGCY	1 cut(s)	428
HapII	CCGG	5 cut(s)	12, 363, 2399, 2465, 2767
HgaI	GACGC	4 cut(s)	377, 485, 551, 2282
HhaI	GCGC	4 cut(s)	373, 427, 453, 1121
Hin1I	GRCGYC	2 cut(s)	477, 562
Hin6I	GCGC	4 cut(s)	371, 425, 451, 1119
HinP1I	GCGC	4 cut(s)	371, 425, 451, 1119
HincII	GTYRAC	3 cut(s)	1971, 2839, 2869
HindII	GTYRAC	3 cut(s)	1971, 2839, 2869
HindIII	AAGCTT	1 cut(s)	2512
HinfI	GANTC	9 cut(s)	269, 656, 884, 1361, 1513, 1720, 2287, 2336, 2363
HpaII	CCGG	5 cut(s)	12, 363, 2399, 2465, 2767
HphI	GGTGA	5 cut(s)	38, 145, 251, 1315, 1946
Hpy99I	CGWCG	6 cut(s)	371, 409, 479, 515, 548, 2828
HpyAV	CCTTC	8 cut(s)	53, 101, 160, 208, 266, 551, 1330, 1435
HpyCH4IV	ACGT	7 cut(s)	91, 198, 407, 519, 1390, 1983, 2303
HpyF10VI	GCNNNNNNNGC	4 cut(s)	1094, 1118, 2212, 2557
HpySE526I	ACGT	7 cut(s)	91, 198, 407, 519, 1390, 1983, 2303
Hsp92I	GRCGYC	2 cut(s)	477, 562
HspAI	GCGC	4 cut(s)	371, 425, 451, 1119
KflI	GGGWCCC	1 cut(s)	1026
Kpn2I	TCCGGA	1 cut(s)	11
KroI	GCCGGC	1 cut(s)	2766
KroNI	GCCGGC	1 cut(s)	2768
KspI	CCGCGG	1 cut(s)	1033
Kzo9I	GATC	9 cut(s)	8, 19, 75, 182, 331, 487, 540, 2609, 2819
LguI	GCTCTTC	1 cut(s)	930
LmnI	GCTCC	6 cut(s)	365, 619, 1817, 2132, 2281, 2777
Lsp1109I	GCAGC	5 cut(s)	375, 568, 814, 1133, 2202
LweI	GCATC	5 cut(s)	860, 1028, 1750, 1772, 2308
MabI	ACCWGGT	1 cut(s)	1060
MaeI	CTAG	7 cut(s)	1641, 1725, 2190, 2231, 2781, 2814, 2953
MaeII	ACGT	7 cut(s)	91, 198, 407, 519, 1390, 1983, 2303
MaeIII	GTNAC	8 cut(s)	713, 1952, 1959, 1986, 2137, 2534, 2662, 2731
MalI	GATC	9 cut(s)	10, 21, 77, 184, 333, 489, 542, 2611, 2821
MbiI	CCGCTC	1 cut(s)	466
MboI	GATC	9 cut(s)	8, 19, 75, 182, 331, 487, 540, 2609, 2819
MboII	GAAGA	6 cut(s)	289, 665, 947, 982, 1323, 2941
MfeI	CAATTG	1 cut(s)	1596
MflI	RGATCY	4 cut(s)	8, 19, 2609, 2819
MhlI	GDGCHC	1 cut(s)	519
MlyI	GAGTC	3 cut(s)	665, 1370, 2330
MmeI	TCCRAC	5 cut(s)	60, 167, 273, 585, 2847
Mph1103I	ATGCAT	2 cut(s)	1372, 1765
MroI	TCCGGA	1 cut(s)	11
MroNI	GCCGGC	1 cut(s)	2766
MseI	TTAA	8 cut(s)	666, 678, 788, 924, 1314, 1347, 1781, 2648
MslI	CAYNNNNRTG	4 cut(s)	979, 2223, 2225, 2761
MspA1I	CMGCKG	1 cut(s)	1032
MspI	CCGG	5 cut(s)	12, 363, 2399, 2465, 2767
MunI	CAATTG	1 cut(s)	1596
Mva1269I	GAATGC	1 cut(s)	1372
MvnI	CGCG	3 cut(s)	371, 1032, 1438
MwoI	GCNNNNNNNGC	4 cut(s)	1094, 1118, 2212, 2557
NaeI	GCCGGC	1 cut(s)	2768
NcoI	CCATGG	1 cut(s)	697
NdeI	CATATG	2 cut(s)	2089, 2762
NdeII	GATC	9 cut(s)	8, 19, 75, 182, 331, 487, 540, 2609, 2819
NgoMIV	GCCGGC	1 cut(s)	2766
NheI	GCTAGC	1 cut(s)	2780
NlaIV	GGNNCC	9 cut(s)	10, 21, 631, 673, 897, 1027, 1028, 2644, 2821
NmeAIII	GCCGAG	1 cut(s)	883
NmuCI	GTSAC	4 cut(s)	1952, 1959, 2137, 2534
NsiI	ATGCAT	2 cut(s)	1372, 1765
NspI	RCATGY	2 cut(s)	451, 2396
OliI	CACNNNNGTG	1 cut(s)	2223
PagI	TCATGA	1 cut(s)	966
PceI	AGGCCT	2 cut(s)	1319, 2892
PciSI	GCTCTTC	1 cut(s)	930
PcsI	WCGNNNNNNNCGW	1 cut(s)	483
PctI	GAATGC	1 cut(s)	1372
PdiI	GCCGGC	1 cut(s)	2768
PfeI	GAWTC	6 cut(s)	269, 884, 1513, 1720, 2287, 2363
Pfl23II	CGTACG	3 cut(s)	122, 229, 408
PflMI	CCANNNNNTGG	2 cut(s)	2174, 2863
PfoI	TCCNGGA	2 cut(s)	1147, 1912
PkrI	GCNGC	9 cut(s)	390, 424, 465, 551, 583, 829, 1123, 2217, 2589
Ple19I	CGATCG	1 cut(s)	490
PleI	GAGTC	3 cut(s)	664, 1369, 2330
PpsI	GAGTC	3 cut(s)	664, 1369, 2330
Ppu21I	YACGTR	1 cut(s)	1984
PpuMI	RGGWCCY	2 cut(s)	1026, 1058
PshAI	GACNNNNGTC	1 cut(s)	2745
PsiI	TTATAA	1 cut(s)	1007
Psp5II	RGGWCCY	2 cut(s)	1026, 1058
PspEI	GGTNACC	2 cut(s)	1952, 2662
PspFI	CCCAGC	3 cut(s)	626, 1862, 2059
PspLI	CGTACG	3 cut(s)	122, 229, 408
PspN4I	GGNNCC	9 cut(s)	10, 21, 631, 673, 897, 1027, 1028, 2644, 2821
PspPPI	RGGWCCY	2 cut(s)	1026, 1058
PstI	CTGCAG	1 cut(s)	2317
PstNI	CAGNNNCTG	3 cut(s)	827, 1061, 2729
PsuI	RGATCY	4 cut(s)	8, 19, 2609, 2819
PvuI	CGATCG	1 cut(s)	490
RsaI	GTAC	8 cut(s)	124, 231, 410, 441, 446, 590, 1995, 2040
RsaNI	GTAC	8 cut(s)	123, 230, 409, 440, 445, 589, 1994, 2039
RseI	CAYNNNNRTG	4 cut(s)	979, 2223, 2225, 2761
SacII	CCGCGG	1 cut(s)	1033
SapI	GCTCTTC	1 cut(s)	930
SaqAI	TTAA	8 cut(s)	666, 678, 788, 924, 1314, 1347, 1781, 2648
SatI	GCNGC	9 cut(s)	389, 423, 464, 550, 582, 828, 1122, 2216, 2588
Sau3AI	GATC	9 cut(s)	8, 19, 75, 182, 331, 487, 540, 2609, 2819
SchI	GAGTC	3 cut(s)	665, 1370, 2330
SduI	GDGCHC	1 cut(s)	519
SexAI	ACCWGGT	1 cut(s)	1060
SfaNI	GCATC	5 cut(s)	860, 1028, 1750, 1772, 2308
SfcI	CTRYAG	1 cut(s)	2313
Sfr303I	CCGCGG	1 cut(s)	1033
SgrBI	CCGCGG	1 cut(s)	1033
SinI	GGWCC	5 cut(s)	630, 1026, 1034, 1058, 2747
SmiMI	CAYNNNNRTG	4 cut(s)	979, 2223, 2225, 2761
SmlI	CTYRAG	4 cut(s)	1176, 1755, 1772, 2506
SmoI	CTYRAG	4 cut(s)	1176, 1755, 1772, 2506
SpeI	ACTAGT	1 cut(s)	1640
SseBI	AGGCCT	2 cut(s)	1319, 2892
SspI	AATATT	1 cut(s)	733
SspMI	CTAG	7 cut(s)	1641, 1725, 2190, 2231, 2781, 2814, 2953
StuI	AGGCCT	2 cut(s)	1319, 2892
StyI	CCWWGG	2 cut(s)	697, 1219
TaiI	ACGT	7 cut(s)	94, 201, 410, 522, 1393, 1986, 2306
TaqI	TCGA	8 cut(s)	468, 474, 486, 513, 523, 543, 1364, 2927
TaqII	GACCGA	1 cut(s)	590
TatI	WGTACW	3 cut(s)	444, 1993, 2038
TauI	GCSGC	4 cut(s)	425, 466, 552, 2590
TfiI	GAWTC	6 cut(s)	269, 884, 1513, 1720, 2287, 2363
Tru1I	TTAA	8 cut(s)	666, 678, 788, 924, 1314, 1347, 1781, 2648
Tru9I	TTAA	8 cut(s)	666, 678, 788, 924, 1314, 1347, 1781, 2648
TscAI	CASTG	2 cut(s)	2133, 2272
TseFI	GTSAC	4 cut(s)	1952, 1959, 2137, 2534
TseI	GCWGC	5 cut(s)	388, 581, 827, 1121, 2215
Tsp45I	GTSAC	4 cut(s)	1952, 1959, 2137, 2534
TspGWI	ACGGA	6 cut(s)	12, 427, 649, 1154, 1398, 2757
TspRI	CASTG	2 cut(s)	2133, 2272
Van91I	CCANNNNNTGG	2 cut(s)	2174, 2863
VpaK11BI	GGWCC	5 cut(s)	630, 1026, 1034, 1058, 2747
XapI	RAATTY	3 cut(s)	1297, 2470, 2481
XceI	RCATGY	2 cut(s)	451, 2396
XmiI	GTMKAC	2 cut(s)	2576, 2622
XspI	CTAG	7 cut(s)	1641, 1725, 2190, 2231, 2781, 2814, 2953
Zsp2I	ATGCAT	2 cut(s)	1372, 1765

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.