pycom16g10430

Squamosa promoter-binding-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0008130
Physical Location & Seq
Reverse (-)
7114844 .. 7115997
1154 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 939 bp
ATGGAAGATGGATCAAGGGGCAAGTTGAAGGATCGATCTGGGGTCTCTACGAGGAAGCCATCTTCGAGTACTACATCTTTGAAAAGACCGCGACAACCCAATAACGAAACCAAGATTGCTGCATGCTTGGTTGATGGGTGCAATTCGGACCTTAGCAAATGCCGGGATTATCATCGACGGCATAAAGTATGTGAGCTCCATTCAAAGACCTCAAAAGTTACAATCAAGGGCCAGGAACGGCGGTTCTGTCAGCAGTGCAGCAGGTTCCATTCGTTGGTGGAATTCGATGAAGGAAAAAGAAGCTGCAGAAGACGTCTTGATGGACACAACAAACGTAGGAGGAAGCGTCAGTCAGAATCCATGTCCACAAATTTGGGAAGATTTCTTTCCAATTACCAAGGCACAAGATTCTTATCATTTGGTAATCCACAAATGTGTGCAGCAAATGCTGTCATGAACTCTCCGTGGGGCTACGCATTGAAACCGGAGAATGATTCTGTGCTGCTTGATAACCACTCACAATTAAATGAGACAAGAAGAAGCTCATATACTTACCGAGAAAAACAAATCCCATTTGTGCAAGGCTTTGAATACGCGCACTCAGAAGCTTCTGTCTACCAACCATTTACAAATGCCAACTTTGCACTGGGAAATTCTAGCAACAGCCATAGAATGGTTGCCAATGGATTATACCGCGACAGAGAATCTAATTGTGCTCTCTCTCTTCTGTCATGGACACGAGCAACACAAGAGGAGATTGGGCTGAGCCACACAGCGCAGTCCAGTTCAGTATGCCCGGCTCAGTCCATGATCCCAAGAATCCACTACAATAATGTGGGAGTGCAGGATGAGGCTGCAATTTCTAATTTGGTTAGTGATGGCAACAGCATTGGCAATCTCCACTGCCAGGGCAAGTTTCAGAATTCAAATTATTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SBP

Protein Analysis

313

Amino Acids

35.25

Weight (kDa)

9.58

Isoelectric Point (pI)

55.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SBP PF03110 42 - 116 5.8e-30 SBP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 316
Acc36I ACCTGC 1 cut(s) 252
AccB7I CCANNNNNTGG 2 cut(s) 274, 673
AccI GTMKAC 1 cut(s) 615
AccII CGCG 3 cut(s) 91, 596, 696
AciI CCGC 3 cut(s) 89, 241, 694
AclWI GGATC 3 cut(s) 19, 39, 805
AcsI RAATTY 4 cut(s) 281, 370, 652, 922
AcyI GRCGYC 1 cut(s) 313
AfaI GTAC 1 cut(s) 70
AfiI CCNNNNNNNGG 3 cut(s) 274, 673, 907
AgsI TTSAA 6 cut(s) 28, 82, 204, 481, 590, 927
AjnI CCWGG 2 cut(s) 231, 906
AleI CACNNNNGTG 1 cut(s) 433
AloI GAACNNNNNNTCC 2 cut(s) 227, 259
AluBI AGCT 4 cut(s) 196, 303, 543, 608
AluI AGCT 4 cut(s) 196, 303, 543, 608
Alw21I GWGCWC 2 cut(s) 198, 718
Alw26I GTCTC 2 cut(s) 49, 524
AlwI GGATC 3 cut(s) 19, 39, 805
AoxI GGCC 1 cut(s) 229
ApeKI GCWGC 6 cut(s) 119, 258, 303, 440, 502, 854
ApoI RAATTY 4 cut(s) 281, 370, 652, 922
AspLEI GCGC 2 cut(s) 598, 778
AspS9I GGNCC 2 cut(s) 148, 229
AsuC2I CCSGG 2 cut(s) 164, 797
AvaII GGWCC 1 cut(s) 148
BanII GRGCYC 1 cut(s) 198
BarI GAAGNNNNNNTAC 2 cut(s) 532, 564
BauI CACGAG 1 cut(s) 738
BbsI GAAGAC 1 cut(s) 316
Bbv12I GWGCWC 2 cut(s) 198, 718
BbvI GCAGC 6 cut(s) 106, 270, 290, 452, 489, 841
BccI CCATC 5 cut(s) 2, 67, 128, 314, 872
BceAI ACGGC 2 cut(s) 194, 254
BciT130I CCWGG 2 cut(s) 233, 908
BcnI CCSGG 2 cut(s) 164, 797
BcoDI GTCTC 2 cut(s) 49, 524
BfaI CTAG 1 cut(s) 657
BfmI CTRYAG 1 cut(s) 304
BfuAI ACCTGC 1 cut(s) 252
BisI GCNGC 6 cut(s) 120, 259, 304, 441, 503, 855
BlpI GCTNAGC 1 cut(s) 764
BlsI GCNGC 6 cut(s) 121, 260, 305, 442, 504, 856
BmcAI AGTACT 1 cut(s) 70
Bme1390I CCNGG 4 cut(s) 164, 233, 797, 908
Bme18I GGWCC 1 cut(s) 148
BmgT120I GGNCC 2 cut(s) 148, 229
BmiI GGNNCC 1 cut(s) 266
BmrFI CCNGG 4 cut(s) 164, 233, 797, 908
BmrI ACTGGG 1 cut(s) 656
BmuI ACTGGG 1 cut(s) 656
BpiI GAAGAC 1 cut(s) 316
Bpu10I CCTNAGC 1 cut(s) 152
Bpu1102I GCTNAGC 1 cut(s) 764
BpuMI CCSGG 2 cut(s) 164, 797
Bsa29I ATCGAT 1 cut(s) 34
BsaBI GATNNNNATC 2 cut(s) 171, 412
BsaHI GRCGYC 1 cut(s) 313
BsaI GGTCTC 1 cut(s) 49
BsaJI CCNNGG 3 cut(s) 397, 464, 907
BsaWI WCCGGW 1 cut(s) 484
BsaXI ACNNNNNCTCC 2 cut(s) 331, 361
Bsc4I CCNNNNNNNGG 3 cut(s) 274, 673, 907
Bse1I ACTGG 2 cut(s) 651, 783
Bse8I GATNNNNATC 2 cut(s) 171, 412
BseBI CCWGG 2 cut(s) 233, 908
BseCI ATCGAT 1 cut(s) 34
BseDI CCNNGG 3 cut(s) 397, 464, 907
BseGI GGATG 1 cut(s) 853
BseJI GATNNNNATC 2 cut(s) 171, 412
BseLI CCNNNNNNNGG 3 cut(s) 274, 673, 907
BseMII CTCAG 3 cut(s) 615, 755, 815
BseNI ACTGG 2 cut(s) 651, 783
BseRI GAGGAG 1 cut(s) 767
BseXI GCAGC 6 cut(s) 106, 270, 290, 452, 489, 841
BsgI GTGCAG 3 cut(s) 277, 459, 863
Bsh1236I CGCG 3 cut(s) 91, 596, 696
BshFI GGCC 1 cut(s) 231
BshVI ATCGAT 1 cut(s) 34
BsiHKAI GWGCWC 2 cut(s) 198, 718
BsiSI CCGG 3 cut(s) 163, 485, 797
BslI CCNNNNNNNGG 3 cut(s) 274, 673, 907
BsmAI GTCTC 2 cut(s) 49, 524
BsnI GGCC 1 cut(s) 231
Bso31I GGTCTC 1 cut(s) 49
Bsp1286I GDGCHC 2 cut(s) 198, 718
Bsp143I GATC 4 cut(s) 11, 31, 35, 810
Bsp1720I GCTNAGC 1 cut(s) 764
BspACI CCGC 3 cut(s) 89, 241, 694
BspANI GGCC 1 cut(s) 231
BspCNI CTCAG 3 cut(s) 614, 756, 814
BspDI ATCGAT 1 cut(s) 34
BspFNI CGCG 3 cut(s) 91, 596, 696
BspHI TCATGA 1 cut(s) 453
BspLI GGNNCC 1 cut(s) 266
BspMAI CTGCAG 1 cut(s) 308
BspMI ACCTGC 1 cut(s) 252
BspPI GGATC 3 cut(s) 19, 39, 805
BspTNI GGTCTC 1 cut(s) 49
BsrI ACTGG 2 cut(s) 651, 783
BssECI CCNNGG 3 cut(s) 397, 464, 907
BssMI GATC 4 cut(s) 11, 31, 35, 810
BssNI GRCGYC 1 cut(s) 313
BssSI CACGAG 1 cut(s) 738
BssT1I CCWWGG 1 cut(s) 397
Bst2BI CACGAG 1 cut(s) 738
Bst2UI CCWGG 2 cut(s) 233, 908
Bst6I CTCTTC 1 cut(s) 729
BstACI GRCGYC 1 cut(s) 313
BstAPI GCANNNNNTGC 1 cut(s) 446
BstC8I GCNNGC 1 cut(s) 124
BstDEI CTNAG 4 cut(s) 152, 601, 764, 801
BstDSI CCRYGG 1 cut(s) 464
BstF5I GGATG 1 cut(s) 853
BstFNI CGCG 3 cut(s) 91, 596, 696
BstHHI GCGC 2 cut(s) 598, 778
BstKTI GATC 4 cut(s) 14, 34, 38, 813
BstMAI GTCTC 2 cut(s) 49, 524
BstMBI GATC 4 cut(s) 11, 31, 35, 810
BstMWI GCNNNNNNNGC 2 cut(s) 446, 641
BstNI CCWGG 2 cut(s) 233, 908
BstNSI RCATGY 1 cut(s) 126
BstSCI CCNGG 4 cut(s) 162, 231, 795, 906
BstSFI CTRYAG 1 cut(s) 304
BstUI CGCG 3 cut(s) 91, 596, 696
BstV1I GCAGC 6 cut(s) 106, 270, 290, 452, 489, 841
BstV2I GAAGAC 1 cut(s) 316
BstXI CCANNNNNNTGG 1 cut(s) 373
Bsu15I ATCGAT 1 cut(s) 34
BsuRI GGCC 1 cut(s) 231
BsuTUI ATCGAT 1 cut(s) 34
BtgI CCRYGG 1 cut(s) 464
BtsCI GGATG 1 cut(s) 853
BtsI GCAGTG 2 cut(s) 260, 901
BtsIMutI CAGTG 3 cut(s) 260, 644, 901
BveI ACCTGC 1 cut(s) 252
Cac8I GCNNGC 1 cut(s) 124
CciI TCATGA 1 cut(s) 453
CfoI GCGC 2 cut(s) 598, 778
Cfr13I GGNCC 2 cut(s) 148, 229
ClaI ATCGAT 1 cut(s) 34
CseI GACGC 1 cut(s) 335
Csp6I GTAC 1 cut(s) 69
CviAII CATG 5 cut(s) 123, 361, 454, 732, 808
CviQI GTAC 1 cut(s) 69
DdeI CTNAG 4 cut(s) 152, 601, 764, 801
DpnI GATC 4 cut(s) 13, 33, 37, 812
DpnII GATC 4 cut(s) 11, 31, 35, 810
Eam1104I CTCTTC 1 cut(s) 729
EarI CTCTTC 1 cut(s) 729
Ecl136II GAGCTC 1 cut(s) 196
Eco130I CCWWGG 1 cut(s) 397
Eco24I GRGCYC 1 cut(s) 198
Eco31I GGTCTC 1 cut(s) 49
Eco47I GGWCC 1 cut(s) 148
Eco53kI GAGCTC 1 cut(s) 196
EcoICRI GAGCTC 1 cut(s) 196
EcoRI GAATTC 2 cut(s) 281, 922
EcoRII CCWGG 2 cut(s) 231, 906
EcoT14I CCWWGG 1 cut(s) 397
EcoT38I GRGCYC 1 cut(s) 198
ErhI CCWWGG 1 cut(s) 397
FaeI CATG 5 cut(s) 126, 364, 457, 735, 811
FatI CATG 5 cut(s) 122, 360, 453, 731, 807
FblI GTMKAC 1 cut(s) 615
Fnu4HI GCNGC 6 cut(s) 120, 259, 304, 441, 503, 855
FokI GGATG 1 cut(s) 860
FriOI GRGCYC 1 cut(s) 198
Fsp4HI GCNGC 6 cut(s) 120, 259, 304, 441, 503, 855
FspBI CTAG 1 cut(s) 657
GlaI GCGC 2 cut(s) 597, 777
GluI GCNGC 6 cut(s) 120, 259, 304, 441, 503, 855
HaeIII GGCC 1 cut(s) 231
HapII CCGG 3 cut(s) 163, 485, 797
HgaI GACGC 1 cut(s) 335
HhaI GCGC 2 cut(s) 598, 778
Hin1I GRCGYC 1 cut(s) 313
Hin1II CATG 5 cut(s) 126, 364, 457, 735, 811
Hin6I GCGC 2 cut(s) 596, 776
HinP1I GCGC 2 cut(s) 596, 776
HindIII AAGCTT 1 cut(s) 606
HinfI GANTC 5 cut(s) 356, 408, 494, 704, 819
HpaII CCGG 3 cut(s) 163, 485, 797
Hpy166II GTNNAC 2 cut(s) 366, 616
Hpy188I TCNGA 4 cut(s) 148, 355, 604, 921
Hpy188III TCNNGA 3 cut(s) 317, 454, 936
Hpy8I GTNNAC 2 cut(s) 366, 616
Hpy99I CGWCG 1 cut(s) 180
HpyAV CCTTC 2 cut(s) 22, 284
HpyCH4IV ACGT 2 cut(s) 313, 334
HpyCH4V TGCA 9 cut(s) 122, 141, 258, 306, 440, 580, 644, 844, 857
HpyF10VI GCNNNNNNNGC 2 cut(s) 446, 641
HpyF3I CTNAG 4 cut(s) 152, 601, 764, 801
HpySE526I ACGT 2 cut(s) 313, 334
Hsp92I GRCGYC 1 cut(s) 313
Hsp92II CATG 5 cut(s) 126, 364, 457, 735, 811
HspAI GCGC 2 cut(s) 596, 776
Kzo9I GATC 4 cut(s) 11, 31, 35, 810
LmnI GCTCC 1 cut(s) 201
Lsp1109I GCAGC 6 cut(s) 106, 270, 290, 452, 489, 841
MaeI CTAG 1 cut(s) 657
MaeII ACGT 2 cut(s) 313, 334
MaeIII GTNAC 1 cut(s) 217
MalI GATC 4 cut(s) 13, 33, 37, 812
MboI GATC 4 cut(s) 11, 31, 35, 810
MboII GAAGA 6 cut(s) 17, 54, 321, 390, 549, 716
MhlI GDGCHC 2 cut(s) 198, 718
MnlI CCTC 5 cut(s) 45, 220, 333, 745, 844
MseI TTAA 1 cut(s) 524
MslI CAYNNNNRTG 1 cut(s) 433
MspI CCGG 3 cut(s) 163, 485, 797
MspR9I CCNGG 4 cut(s) 164, 233, 797, 908
MvaI CCWGG 2 cut(s) 233, 908
MvnI CGCG 3 cut(s) 91, 596, 696
MwoI GCNNNNNNNGC 2 cut(s) 446, 641
NciI CCSGG 2 cut(s) 164, 797
NdeII GATC 4 cut(s) 11, 31, 35, 810
NlaIII CATG 5 cut(s) 126, 364, 457, 735, 811
NlaIV GGNNCC 1 cut(s) 266
NspI RCATGY 1 cut(s) 126
OliI CACNNNNGTG 1 cut(s) 433
PaeI GCATGC 1 cut(s) 126
PagI TCATGA 1 cut(s) 453
PfeI GAWTC 5 cut(s) 356, 408, 494, 704, 819
PflMI CCANNNNNTGG 2 cut(s) 274, 673
PkrI GCNGC 6 cut(s) 121, 260, 305, 442, 504, 856
Psp124BI GAGCTC 1 cut(s) 198
Psp6I CCWGG 2 cut(s) 231, 906
PspGI CCWGG 2 cut(s) 231, 906
PspN4I GGNNCC 1 cut(s) 266
PspPI GGNCC 2 cut(s) 148, 229
PstI CTGCAG 1 cut(s) 308
RsaI GTAC 1 cut(s) 70
RsaNI GTAC 1 cut(s) 69
RseI CAYNNNNRTG 1 cut(s) 433
SacI GAGCTC 1 cut(s) 198
SaqAI TTAA 1 cut(s) 524
SatI GCNGC 6 cut(s) 120, 259, 304, 441, 503, 855
Sau3AI GATC 4 cut(s) 11, 31, 35, 810
Sau96I GGNCC 2 cut(s) 148, 229
ScaI AGTACT 1 cut(s) 70
ScrFI CCNGG 4 cut(s) 164, 233, 797, 908
SduI GDGCHC 2 cut(s) 198, 718
SetI ASST 9 cut(s) 153, 198, 212, 266, 305, 316, 337, 545, 610
SfcI CTRYAG 1 cut(s) 304
SinI GGWCC 1 cut(s) 148
SmiMI CAYNNNNRTG 1 cut(s) 433
SphI GCATGC 1 cut(s) 126
SsiI CCGC 3 cut(s) 89, 241, 694
SspMI CTAG 1 cut(s) 657
SstI GAGCTC 1 cut(s) 198
StyD4I CCNGG 4 cut(s) 162, 231, 795, 906
StyI CCWWGG 1 cut(s) 397
TaiI ACGT 2 cut(s) 316, 337
TaqI TCGA 4 cut(s) 34, 65, 175, 285
TatI WGTACW 1 cut(s) 68
TfiI GAWTC 5 cut(s) 356, 408, 494, 704, 819
Tru1I TTAA 1 cut(s) 524
Tru9I TTAA 1 cut(s) 524
TscAI CASTG 3 cut(s) 260, 651, 908
TseI GCWGC 6 cut(s) 119, 258, 303, 440, 502, 854
TspDTI ATGAA 2 cut(s) 303, 470
TspGWI ACGGA 1 cut(s) 453
TspRI CASTG 3 cut(s) 260, 651, 908
Van91I CCANNNNNTGG 2 cut(s) 274, 673
VpaK11BI GGWCC 1 cut(s) 148
XapI RAATTY 4 cut(s) 281, 370, 652, 922
XceI RCATGY 1 cut(s) 126
XcmI CCANNNNNNNNNTGG 1 cut(s) 643
XmiI GTMKAC 1 cut(s) 615
XspI CTAG 1 cut(s) 657
ZraI GACGTC 1 cut(s) 314
ZrmI AGTACT 1 cut(s) 70
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.