pycom16g11150

Uncharacterized integral membrane protein (DUF2301)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0013259
Physical Location & Seq
Forward (+)
7743805 .. 7745641
1837 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 969 bp
ATGAACAGAGCAAATGGGATTTTTATGCATAATAAATTAACTGAAATTAGCAAAGCCGTAGGTTGCCTTAATTACCCAAAAATGGCTTCCAAAATACTTTGCGTGTGTGCAAGTGCAACCATCGTCCCTCCTCCTCCACCTCCTGTTGCAAAAGTTAGCAGTTCAAATAATATTTCACAGATTTTTCTTTCTTCTTCTGCAGATAAAGCTTCCGTTCGGCTTCAATCCTTCAAGTGCAAAGCTCTCGGAGAAAGCTCTCAGCCGCAAACCTCCTTGGCTGAAACCGTCTATCAGGGAGTTTACGGTCCTTGGTACGTCGACTCCACCGACGTTCGAGAGTTTCCAACTCTGGAAATTCACATTCTTCAACATTTATCGGTGTGGCAGGTTATTTTGTACAGGTCAGGGCTAGTGACTGCAGCTACATCATTTGTAGTTGCAGCTTCTGCTGCATTTTTGCCTGACTGCTTCTTCTTAACTGGTATTTTGAAACACAATCTCGATTTCGTGTATGCAATCGGAGCTGGTGGACTGGGGCTGTCATTGTTTCTGATTCATATATATTTGACTGAAATTAAGCGCACTCTGCAAGCATTTTGTGGGCTTGGTGTTGCCGGGTCTTTAGCAACATATGCATGCTTGGCTAGCCCAGCGGGAGAGGGTTTGGTGCAGTATGTTGTGGATAACCCAATTGCTATCTGGTTTGTTGGCCCTCTCTTTGCTTCTCTCACAGGACTAGTCTTCAAGGAAGAGTGGACTGGAGGACTGATGGACGATGGAGCAAAACTTTCCTTGCTCAGTTCTTGGATAGCCTTGTTTGTGATTTTTGCTGGAAGGAAGTTCACTCAACCGATCAAGGATGACATTGGCTACAAGTCCGTCTTTACATTCAATTCTCTGCCTGAAGATGAGAAGAAGGCCTTGATTCAGAAGCTTGAGCAGCAGAAGTTCAATCAGGATCTCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2301

Protein Analysis

323

Amino Acids

35.01

Weight (kDa)

6.94

Isoelectric Point (pI)

44.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2301 PF10063 180 - 297 2.3e-26 Uncharacterized integral membrane protein (DUF2301)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013259)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28140
fragaria_vesca FvH4_4g22640 FvH4_4g22640
malus_domestica MD13G1130900.v1.1
prunus_persica Prupe.1G214800_v2.0.a1
pyrus_communis pycom13g11420 pycom16g11150
rosa_chinensis RchiOBHm_Chr4g0428871
rosa_laevigata RLG00000007118
rosa_multiflora Rmu_co8417267.1_g000001 Rmu_sc0008612.1_g000007
rosa_roxburghii Rroxscaffold_5G00370550
rosa_rugosa Rorug04G0229900
rosa_samantha Rh4AG286600 Rh4BG292400 Rh4CG308400 Rh4DG289600
rosa_wichuraiana Rw4G024850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 376
AccI GTMKAC 1 cut(s) 318
AciI CCGC 2 cut(s) 263, 653
AclWI GGATC 1 cut(s) 966
AcsI RAATTY 1 cut(s) 354
AcuI CTGAAG 1 cut(s) 924
AfaI GTAC 2 cut(s) 314, 398
AfiI CCNNNNNNNGG 1 cut(s) 82
AgsI TTSAA 8 cut(s) 165, 224, 232, 368, 490, 745, 892, 952
AhlI ACTAGT 1 cut(s) 736
AjuI GAANNNNNNNTTGG 2 cut(s) 70, 102
AluBI AGCT 7 cut(s) 209, 242, 255, 422, 443, 524, 934
AluI AGCT 7 cut(s) 209, 242, 255, 422, 443, 524, 934
AlwI GGATC 1 cut(s) 966
AlwNI CAGNNNCTG 1 cut(s) 446
AoxI GGCC 2 cut(s) 709, 918
ApeKI GCWGC 4 cut(s) 419, 440, 449, 940
ApoI RAATTY 1 cut(s) 354
AspLEI GCGC 1 cut(s) 582
AspS9I GGNCC 2 cut(s) 305, 710
AsuC2I CCSGG 1 cut(s) 616
AsuNHI GCTAGC 1 cut(s) 644
AvaII GGWCC 1 cut(s) 305
BbsI GAAGAC 1 cut(s) 733
BbvI GCAGC 4 cut(s) 431, 436, 452, 952
BccI CCATC 3 cut(s) 128, 763, 770
BceAI ACGGC 1 cut(s) 41
BcgI CGANNNNNNTGC 2 cut(s) 226, 260
BcnI CCSGG 1 cut(s) 616
BcuI ACTAGT 1 cut(s) 736
BfaI CTAG 3 cut(s) 410, 645, 737
BfmI CTRYAG 2 cut(s) 198, 417
BfuAI ACCTGC 1 cut(s) 376
BisI GCNGC 5 cut(s) 263, 420, 441, 450, 941
BlsI GCNGC 5 cut(s) 264, 421, 442, 451, 942
Bme1390I CCNGG 1 cut(s) 616
Bme18I GGWCC 1 cut(s) 305
BmgT120I GGNCC 2 cut(s) 305, 710
BmrFI CCNGG 1 cut(s) 616
BmrI ACTGGG 1 cut(s) 542
BmtI GCTAGC 1 cut(s) 648
BmuI ACTGGG 1 cut(s) 542
BpiI GAAGAC 1 cut(s) 733
BpmI CTGGAG 1 cut(s) 780
BpuEI CTTGAG 1 cut(s) 956
BpuMI CCSGG 1 cut(s) 616
BsaJI CCNNGG 2 cut(s) 273, 308
BsaXI ACNNNNNCTCC 2 cut(s) 305, 335
Bsc4I CCNNNNNNNGG 1 cut(s) 82
Bse1I ACTGG 3 cut(s) 484, 537, 763
BseDI CCNNGG 2 cut(s) 273, 308
BseGI GGATG 1 cut(s) 865
BseLI CCNNNNNNNGG 1 cut(s) 82
BseMII CTCAG 2 cut(s) 272, 811
BseNI ACTGG 3 cut(s) 484, 537, 763
BseRI GAGGAG 2 cut(s) 120, 123
BseXI GCAGC 4 cut(s) 431, 436, 452, 952
BseYI CCCAGC 1 cut(s) 649
BsgI GTGCAG 1 cut(s) 689
BshFI GGCC 2 cut(s) 711, 920
BsiSI CCGG 1 cut(s) 615
BslFI GGGAC 1 cut(s) 110
BslI CCNNNNNNNGG 1 cut(s) 82
BsmFI GGGAC 1 cut(s) 110
BsnI GGCC 2 cut(s) 711, 920
Bsp1407I TGTACA 1 cut(s) 396
Bsp143I GATC 2 cut(s) 852, 958
BspACI CCGC 2 cut(s) 263, 653
BspANI GGCC 2 cut(s) 711, 920
BspCNI CTCAG 2 cut(s) 271, 810
BspMAI CTGCAG 2 cut(s) 202, 421
BspMI ACCTGC 1 cut(s) 376
BspOI GCTAGC 1 cut(s) 648
BspPI GGATC 1 cut(s) 966
BsrGI TGTACA 1 cut(s) 396
BsrI ACTGG 3 cut(s) 484, 537, 763
BssECI CCNNGG 2 cut(s) 273, 308
BssMI GATC 2 cut(s) 852, 958
BssT1I CCWWGG 2 cut(s) 273, 308
Bst4CI ACNGT 2 cut(s) 286, 305
Bst6I CTCTTC 1 cut(s) 744
BstAPI GCANNNNNTGC 2 cut(s) 446, 632
BstAUI TGTACA 1 cut(s) 396
BstC8I GCNNGC 3 cut(s) 591, 637, 646
BstDEI CTNAG 2 cut(s) 258, 797
BstF5I GGATG 1 cut(s) 865
BstHHI GCGC 1 cut(s) 582
BstKTI GATC 2 cut(s) 855, 961
BstMBI GATC 2 cut(s) 852, 958
BstNSI RCATGY 1 cut(s) 639
BstSCI CCNGG 1 cut(s) 614
BstSFI CTRYAG 2 cut(s) 198, 417
BstV1I GCAGC 4 cut(s) 431, 436, 452, 952
BstV2I GAAGAC 1 cut(s) 733
BstX2I RGATCY 1 cut(s) 958
BstYI RGATCY 1 cut(s) 958
BsuRI GGCC 2 cut(s) 711, 920
BtsCI GGATG 1 cut(s) 865
BveI ACCTGC 1 cut(s) 376
Cac8I GCNNGC 3 cut(s) 591, 637, 646
CaiI CAGNNNCTG 1 cut(s) 446
CfoI GCGC 1 cut(s) 582
Cfr13I GGNCC 2 cut(s) 305, 710
Csp6I GTAC 2 cut(s) 313, 397
CviAII CATG 1 cut(s) 636
CviQI GTAC 2 cut(s) 313, 397
DdeI CTNAG 2 cut(s) 258, 797
DpnI GATC 2 cut(s) 854, 960
DpnII GATC 2 cut(s) 852, 958
Eam1104I CTCTTC 1 cut(s) 744
EarI CTCTTC 1 cut(s) 744
Eco130I CCWWGG 2 cut(s) 273, 308
Eco147I AGGCCT 1 cut(s) 920
Eco47I GGWCC 1 cut(s) 305
Eco57I CTGAAG 1 cut(s) 924
EcoT14I CCWWGG 2 cut(s) 273, 308
EcoT22I ATGCAT 2 cut(s) 30, 637
ErhI CCWWGG 2 cut(s) 273, 308
FaeI CATG 1 cut(s) 639
FalI AAGNNNNNCTT 4 cut(s) 866, 898, 905, 937
FaqI GGGAC 1 cut(s) 110
FatI CATG 1 cut(s) 635
FauI CCCGC 1 cut(s) 646
FauNDI CATATG 1 cut(s) 631
FblI GTMKAC 1 cut(s) 318
Fnu4HI GCNGC 5 cut(s) 263, 420, 441, 450, 941
FokI GGATG 1 cut(s) 872
Fsp4HI GCNGC 5 cut(s) 263, 420, 441, 450, 941
FspBI CTAG 3 cut(s) 410, 645, 737
GlaI GCGC 1 cut(s) 581
GluI GCNGC 5 cut(s) 263, 420, 441, 450, 941
GsaI CCCAGC 1 cut(s) 653
GsuI CTGGAG 1 cut(s) 780
HaeIII GGCC 2 cut(s) 711, 920
HapII CCGG 1 cut(s) 615
HhaI GCGC 1 cut(s) 582
Hin1II CATG 1 cut(s) 639
Hin6I GCGC 1 cut(s) 580
HinP1I GCGC 1 cut(s) 580
HincII GTYRAC 1 cut(s) 319
HindII GTYRAC 1 cut(s) 319
HindIII AAGCTT 2 cut(s) 207, 932
HinfI GANTC 3 cut(s) 320, 553, 925
HpaII CCGG 1 cut(s) 615
Hpy166II GTNNAC 5 cut(s) 301, 319, 530, 756, 843
Hpy188I TCNGA 4 cut(s) 248, 521, 552, 930
Hpy188III TCNNGA 5 cut(s) 335, 350, 500, 956, 962
Hpy8I GTNNAC 5 cut(s) 301, 319, 530, 756, 843
Hpy99I CGWCG 2 cut(s) 320, 332
HpyAV CCTTC 3 cut(s) 238, 828, 910
HpyCH4III ACNGT 2 cut(s) 286, 305
HpyCH4IV ACGT 2 cut(s) 315, 330
HpyF3I CTNAG 2 cut(s) 258, 797
HpySE526I ACGT 2 cut(s) 315, 330
Hsp92II CATG 1 cut(s) 639
HspAI GCGC 1 cut(s) 580
Kzo9I GATC 2 cut(s) 852, 958
LmnI GCTCC 2 cut(s) 521, 779
Lsp1109I GCAGC 4 cut(s) 431, 436, 452, 952
MaeI CTAG 3 cut(s) 410, 645, 737
MaeII ACGT 2 cut(s) 315, 330
MaeIII GTNAC 1 cut(s) 412
MalI GATC 2 cut(s) 854, 960
MboI GATC 2 cut(s) 852, 958
MboII GAAGA 8 cut(s) 183, 186, 356, 463, 733, 761, 917, 925
MfeI CAATTG 1 cut(s) 690
MflI RGATCY 1 cut(s) 958
MluCI AATT 7 cut(s) 35, 45, 70, 354, 573, 690, 892
MlyI GAGTC 1 cut(s) 314
MmeI TCCRAC 1 cut(s) 368
MnlI CCTC 8 cut(s) 138, 141, 144, 150, 280, 652, 723, 755
Mph1103I ATGCAT 2 cut(s) 30, 637
MseI TTAA 4 cut(s) 38, 69, 476, 576
MslI CAYNNNNRTG 1 cut(s) 634
MspA1I CMGCKG 1 cut(s) 653
MspI CCGG 1 cut(s) 615
MspR9I CCNGG 1 cut(s) 616
MunI CAATTG 1 cut(s) 690
NciI CCSGG 1 cut(s) 616
NdeI CATATG 1 cut(s) 631
NdeII GATC 2 cut(s) 852, 958
NheI GCTAGC 1 cut(s) 644
NlaIII CATG 1 cut(s) 639
NmuCI GTSAC 1 cut(s) 412
NsiI ATGCAT 2 cut(s) 30, 637
NspI RCATGY 1 cut(s) 639
PaeI GCATGC 1 cut(s) 639
PceI AGGCCT 1 cut(s) 920
PcsI WCGNNNNNNNCGW 1 cut(s) 324
PfeI GAWTC 2 cut(s) 553, 925
PkrI GCNGC 5 cut(s) 264, 421, 442, 451, 942
PleI GAGTC 1 cut(s) 314
PpsI GAGTC 1 cut(s) 314
PspFI CCCAGC 1 cut(s) 649
PspPI GGNCC 2 cut(s) 305, 710
PstI CTGCAG 2 cut(s) 202, 421
PstNI CAGNNNCTG 1 cut(s) 446
PsuI RGATCY 1 cut(s) 958
RsaI GTAC 2 cut(s) 314, 398
RsaNI GTAC 2 cut(s) 313, 397
RseI CAYNNNNRTG 1 cut(s) 634
SalI GTCGAC 1 cut(s) 317
SaqAI TTAA 4 cut(s) 38, 69, 476, 576
SatI GCNGC 5 cut(s) 263, 420, 441, 450, 941
Sau3AI GATC 2 cut(s) 852, 958
Sau96I GGNCC 2 cut(s) 305, 710
SchI GAGTC 1 cut(s) 314
ScrFI CCNGG 1 cut(s) 616
SfcI CTRYAG 2 cut(s) 198, 417
SinI GGWCC 1 cut(s) 305
SmiMI CAYNNNNRTG 1 cut(s) 634
SmlI CTYRAG 1 cut(s) 935
SmoI CTYRAG 1 cut(s) 935
SpeI ACTAGT 1 cut(s) 736
SphI GCATGC 1 cut(s) 639
Sse9I AATT 7 cut(s) 35, 45, 70, 354, 573, 690, 892
SseBI AGGCCT 1 cut(s) 920
SsiI CCGC 2 cut(s) 263, 653
SspI AATATT 1 cut(s) 172
SspMI CTAG 3 cut(s) 410, 645, 737
StuI AGGCCT 1 cut(s) 920
StyD4I CCNGG 1 cut(s) 614
StyI CCWWGG 2 cut(s) 273, 308
TaaI ACNGT 2 cut(s) 286, 305
TaiI ACGT 2 cut(s) 318, 333
TaqI TCGA 4 cut(s) 318, 334, 501, 963
TasI AATT 7 cut(s) 35, 45, 70, 354, 573, 690, 892
TatI WGTACW 1 cut(s) 396
TauI GCSGC 1 cut(s) 265
TfiI GAWTC 2 cut(s) 553, 925
Tru1I TTAA 4 cut(s) 38, 69, 476, 576
Tru9I TTAA 4 cut(s) 38, 69, 476, 576
TseFI GTSAC 1 cut(s) 412
TseI GCWGC 4 cut(s) 419, 440, 449, 940
Tsp45I GTSAC 1 cut(s) 412
TspDTI ATGAA 2 cut(s) 17, 545
TspGWI ACGGA 2 cut(s) 202, 868
VpaK11BI GGWCC 1 cut(s) 305
XapI RAATTY 1 cut(s) 354
XceI RCATGY 1 cut(s) 639
XcmI CCANNNNNNNNNTGG 1 cut(s) 696
XmiI GTMKAC 1 cut(s) 318
XspI CTAG 3 cut(s) 410, 645, 737
Zsp2I ATGCAT 2 cut(s) 30, 637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.