pycom16g11630

GPI ethanolamine phosphate transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0009386
Physical Location & Seq
Forward (+)
8081696 .. 8086236
4541 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1635 bp
ATGGGTAAAGGAATATGGATTCACTGGGCGATCATGGCGCTTCACGTCGTCGCCATTCTCTTATTCACCAGAGGTTTTCTTCTCACCCGGACGGAGCTTCCCTTTTACAGCAACTGCTCCGACGCTTCCCAATCGCCTTGCTCATACTCCGTTCCAGAAACCCAGAACCGGAATGACACCGTTGAGCCGAATCAGCAGCGTTGCTGGAGCAAGCCTGCTGTTGGGCGGCTTGTAATCATCGTTTTCGACGCTCTCAGGTTTGATTTTGTAGCTCCGAGTACTTTCTTTCAGGAATTGAAGCCATGGATGAACAAATTACAGCTTATACAAGACCTAGCAGCTAGAAATGCATCGTCTGCTCTGATTTTCAAAGCCATAGCTGATCCGCCCACGACGAGTTTGCAGCGTTTGAAGGGCTTGACAACCGGTGGATTGCCGACTTTTATTGATGTTGGGAACAGCTTTGGTGCTCCAGCTATAGTTGAAGATAACTTGATACATCAGTTGGCTAAAAATGGAAAGCGAGTTGTGATGATGGGGGATGATACATGGACGCAGTTGTTTCCTCATCACTTTGAAAAATCTTTTCCTTACCCCTCTTTTAATGTTAGAGATCTTGATACGGTGGACAACGGATGCATTGACCACCTTCTTCCATTCTTGTACCAAGAAGATTGGGATGTTCTTATCGCACACTTTCTTGGAGTGGATCATGCTGGGCACATATTTGGGGTTGATTCTGTGCAAATGATAGAAAAGTTGGAGCAATATAACAACGTCTTGCTGAAAGTAGTTGAAGCATTGGAGAGGCAGTCTGCGCCAGGGGGCATACATGACAATACTCTTCTACTTGTGATGGGCGATCATGGTCAGACTGTGAATGGAGATCATGGTGGAGGAAGTGCTGAAGAGGTTGAGACATCCATGTTTGCTGTGAGCTTTAAGAATCCTCCATCTCCCATACCATTAGAGTTTGATACTTCATCTTGTGAACTAGACTTGGATGGGAGGAATATATGTGCCAGTTCCTTCCAGCAGCTTGACTTTGCAGTGACAATATCGGCGCTTCTTGGTATCCCCTTTCCTTTTGGAAGTATTGGGCGAGTTAATCCTCAGCTATACGCTTTAGGTGCTGGAACATGGAATTTTGAAGATAGTGTGGGCAATTCCCAAAGTCATTCAAAGTTGGAACAATGGATGCTGAATTATGCCAATGTACTCTGCACCAATTCTTGGCAGGTAAAAAGATATATAGATATTTATTCAGCTTCATCAGTTATTGGATTTTCCCACGAAGACTTGTTACACATAGCAGACATGTATGCTAAAGCAGAAGAGAAATGGTCACATACTACAAAGAAATTGCTGTCACATGAAAAGGAAAGCCAGAATGAATTATTGCCTGCTCTTAAGAGGCAAATTGATTTGTATTCTGATTTCCTGGCAAGTGTTGCTGAACTTGCACGCTCCAAATGGACCGAGTTTAATTTAAAGATGATGGGTGCTGGACTTGGCATTATGTTGATGTCACTTCTTATGCATTTTATTGCAATTAAGAAAGCAGCTTCCTCTCGAATAGTTTTATCTTGGAAGAAGGAAAAGCTGCATGCTTTCTTTTGGCCACAACTGGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
Phosphodiest

Protein Analysis

545

Amino Acids

60.87

Weight (kDa)

5.67

Isoelectric Point (pI)

39.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Phosphodiest PF01663 214 - 293 6.9e-09 Type I phosphodiesterase / nucleotide pyrophosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1228
AccB7I CCANNNNNTGG 1 cut(s) 1233
AciI CCGC 2 cut(s) 226, 386
AclWI GGATC 2 cut(s) 377, 717
AcoI YGGCCR 1 cut(s) 1619
AcsI RAATTY 1 cut(s) 1144
AcuI CTGAAG 1 cut(s) 927
AfaI GTAC 3 cut(s) 280, 665, 1218
AfiI CCNNNNNNNGG 3 cut(s) 168, 221, 1233
AflII CTTAAG 1 cut(s) 1409
AflIII ACRYGT 1 cut(s) 1317
AgeI ACCGGT 1 cut(s) 425
AgsI TTSAA 8 cut(s) 298, 370, 412, 485, 578, 797, 1151, 1182
AjiI CACGTC 1 cut(s) 46
AjnI CCWGG 2 cut(s) 820, 1440
Alw21I GWGCWC 1 cut(s) 472
Alw26I GTCTC 1 cut(s) 911
AlwI GGATC 2 cut(s) 377, 717
AlwNI CAGNNNCTG 1 cut(s) 114
AoxI GGCC 1 cut(s) 1619
ApeKI GCWGC 6 cut(s) 196, 338, 403, 1036, 1562, 1603
ApoI RAATTY 1 cut(s) 1144
AsiGI ACCGGT 1 cut(s) 425
AspLEI GCGC 3 cut(s) 40, 820, 1066
AspS9I GGNCC 1 cut(s) 1476
AsuC2I CCSGG 1 cut(s) 88
AsuHPI GGTGA 2 cut(s) 58, 76
AvaII GGWCC 1 cut(s) 1476
BaeGI GKGCMC 1 cut(s) 723
BaeI ACNNNNGTAYC 2 cut(s) 488, 521
BalI TGGCCA 1 cut(s) 1621
BarI GAAGNNNNNNTAC 2 cut(s) 1287, 1319
BbsI GAAGAC 1 cut(s) 1302
Bbv12I GWGCWC 1 cut(s) 472
BbvCI CCTCAGC 1 cut(s) 1113
BbvI GCAGC 6 cut(s) 208, 350, 415, 1048, 1574, 1590
BccI CCATC 5 cut(s) 529, 850, 961, 998, 1492
BcgI CGANNNNNNTGC 2 cut(s) 382, 416
BciT130I CCWGG 2 cut(s) 822, 1442
BciVI GTATCC 1 cut(s) 1085
BcnI CCSGG 1 cut(s) 88
BcoDI GTCTC 1 cut(s) 911
BfaI CTAG 3 cut(s) 335, 342, 995
BfmI CTRYAG 1 cut(s) 477
BfoI RGCGCY 2 cut(s) 41, 1067
BfrI CTTAAG 1 cut(s) 1409
BfuAI ACCTGC 1 cut(s) 1228
BfuI GTATCC 1 cut(s) 1085
BglII AGATCT 1 cut(s) 613
BisI GCNGC 7 cut(s) 197, 227, 339, 404, 1037, 1563, 1604
BlsI GCNGC 7 cut(s) 198, 228, 340, 405, 1038, 1564, 1605
BmcAI AGTACT 1 cut(s) 280
Bme1390I CCNGG 3 cut(s) 88, 822, 1442
Bme18I GGWCC 1 cut(s) 1476
BmgBI CACGTC 1 cut(s) 46
BmgT120I GGNCC 1 cut(s) 1476
BmrFI CCNGG 3 cut(s) 88, 822, 1442
BmrI ACTGGG 1 cut(s) 34
BmsI GCATC 3 cut(s) 359, 626, 1188
BmuI ACTGGG 1 cut(s) 34
BpiI GAAGAC 1 cut(s) 1302
BpmI CTGGAG 2 cut(s) 226, 456
Bpu10I CCTNAGC 1 cut(s) 1113
BpuMI CCSGG 1 cut(s) 88
BsaJI CCNNGG 2 cut(s) 302, 821
BsaWI WCCGGW 2 cut(s) 168, 425
BsaXI ACNNNNNCTCC 2 cut(s) 797, 827
Bsc4I CCNNNNNNNGG 3 cut(s) 168, 221, 1233
Bse118I RCCGGY 1 cut(s) 425
Bse1I ACTGG 3 cut(s) 29, 1023, 1632
BseBI CCWGG 2 cut(s) 822, 1442
BseDI CCNNGG 2 cut(s) 302, 821
BseGI GGATG 7 cut(s) 312, 547, 641, 685, 920, 1009, 1203
BseLI CCNNNNNNNGG 3 cut(s) 168, 221, 1233
BseMII CTCAG 2 cut(s) 268, 1127
BseNI ACTGG 3 cut(s) 29, 1023, 1632
BseSI GKGCMC 1 cut(s) 723
BseXI GCAGC 6 cut(s) 208, 350, 415, 1048, 1574, 1590
BseYI CCCAGC 1 cut(s) 716
BsgI GTGCAG 1 cut(s) 1207
BshFI GGCC 1 cut(s) 1621
BshTI ACCGGT 1 cut(s) 425
BsiHKAI GWGCWC 1 cut(s) 472
BsiSI CCGG 3 cut(s) 88, 169, 426
BslI CCNNNNNNNGG 3 cut(s) 168, 221, 1233
BsmAI GTCTC 1 cut(s) 911
BsnI GGCC 1 cut(s) 1621
Bsp1286I GDGCHC 2 cut(s) 472, 723
Bsp143I GATC 6 cut(s) 30, 382, 613, 709, 862, 886
Bsp19I CCATGG 1 cut(s) 302
BspACI CCGC 2 cut(s) 226, 386
BspANI GGCC 1 cut(s) 1621
BspCNI CTCAG 2 cut(s) 267, 1126
BspMI ACCTGC 1 cut(s) 1228
BspPI GGATC 2 cut(s) 377, 717
BspTI CTTAAG 1 cut(s) 1409
BsrFI RCCGGY 1 cut(s) 425
BsrI ACTGG 3 cut(s) 29, 1023, 1632
BssAI RCCGGY 1 cut(s) 425
BssECI CCNNGG 2 cut(s) 302, 821
BssMI GATC 6 cut(s) 30, 382, 613, 709, 862, 886
BssT1I CCWWGG 1 cut(s) 302
Bst2UI CCWGG 2 cut(s) 822, 1442
Bst4CI ACNGT 3 cut(s) 181, 625, 877
Bst6I CTCTTC 3 cut(s) 849, 903, 1329
BstAFI CTTAAG 1 cut(s) 1409
BstAPI GCANNNNNTGC 2 cut(s) 356, 1451
BstC8I GCNNGC 5 cut(s) 212, 216, 1404, 1465, 1608
BstDEI CTNAG 2 cut(s) 254, 1113
BstDSI CCRYGG 1 cut(s) 302
BstF5I GGATG 7 cut(s) 312, 547, 641, 685, 920, 1009, 1203
BstH2I RGCGCY 2 cut(s) 41, 1067
BstHHI GCGC 3 cut(s) 40, 820, 1066
BstKTI GATC 6 cut(s) 33, 385, 616, 712, 865, 889
BstMAI GTCTC 1 cut(s) 911
BstMBI GATC 6 cut(s) 30, 382, 613, 709, 862, 886
BstMWI GCNNNNNNNGC 8 cut(s) 35, 193, 347, 356, 817, 1130, 1451, 1460
BstNI CCWGG 2 cut(s) 822, 1442
BstNSI RCATGY 2 cut(s) 1321, 1610
BstSCI CCNGG 3 cut(s) 86, 820, 1440
BstSFI CTRYAG 1 cut(s) 477
BstSLI GKGCMC 1 cut(s) 723
BstV1I GCAGC 6 cut(s) 208, 350, 415, 1048, 1574, 1590
BstV2I GAAGAC 1 cut(s) 1302
BstX2I RGATCY 1 cut(s) 613
BstYI RGATCY 1 cut(s) 613
BsuI GTATCC 1 cut(s) 1085
BsuRI GGCC 1 cut(s) 1621
BtgI CCRYGG 1 cut(s) 302
BtrI CACGTC 1 cut(s) 46
BtsCI GGATG 7 cut(s) 312, 547, 641, 685, 920, 1009, 1203
BtsI GCAGTG 1 cut(s) 1056
BtsIMutI CAGTG 2 cut(s) 22, 1056
BveI ACCTGC 1 cut(s) 1228
Cac8I GCNNGC 5 cut(s) 212, 216, 1404, 1465, 1608
CaiI CAGNNNCTG 1 cut(s) 114
CfoI GCGC 3 cut(s) 40, 820, 1066
Cfr10I RCCGGY 1 cut(s) 425
Cfr13I GGNCC 1 cut(s) 1476
CseI GACGC 3 cut(s) 131, 257, 562
Csp6I GTAC 3 cut(s) 279, 664, 1217
CspAI ACCGGT 1 cut(s) 425
CviQI GTAC 3 cut(s) 279, 664, 1217
DdeI CTNAG 2 cut(s) 254, 1113
DpnI GATC 6 cut(s) 32, 384, 615, 711, 864, 888
DpnII GATC 6 cut(s) 30, 382, 613, 709, 862, 886
DraI TTTAAA 1 cut(s) 1491
EaeI YGGCCR 1 cut(s) 1619
Eam1104I CTCTTC 3 cut(s) 849, 903, 1329
EarI CTCTTC 3 cut(s) 849, 903, 1329
EciI GGCGGA 1 cut(s) 375
Eco130I CCWWGG 1 cut(s) 302
Eco47I GGWCC 1 cut(s) 1476
Eco57I CTGAAG 1 cut(s) 927
EcoRII CCWGG 2 cut(s) 820, 1440
EcoT14I CCWWGG 1 cut(s) 302
EcoT22I ATGCAT 3 cut(s) 352, 641, 1542
ErhI CCWWGG 1 cut(s) 302
Fnu4HI GCNGC 7 cut(s) 197, 227, 339, 404, 1037, 1563, 1604
FokI GGATG 7 cut(s) 319, 554, 648, 692, 907, 1016, 1210
Fsp4HI GCNGC 7 cut(s) 197, 227, 339, 404, 1037, 1563, 1604
FspBI CTAG 3 cut(s) 335, 342, 995
GlaI GCGC 3 cut(s) 39, 819, 1065
GluI GCNGC 7 cut(s) 197, 227, 339, 404, 1037, 1563, 1604
GsaI CCCAGC 1 cut(s) 720
GsuI CTGGAG 2 cut(s) 226, 456
HaeII RGCGCY 2 cut(s) 41, 1067
HaeIII GGCC 1 cut(s) 1621
HapII CCGG 3 cut(s) 88, 169, 426
HgaI GACGC 3 cut(s) 131, 257, 562
HhaI GCGC 3 cut(s) 40, 820, 1066
Hin6I GCGC 3 cut(s) 38, 818, 1064
HinP1I GCGC 3 cut(s) 38, 818, 1064
HinfI GANTC 4 cut(s) 19, 190, 737, 946
HpaII CCGG 3 cut(s) 88, 169, 426
HphI GGTGA 2 cut(s) 58, 76
Hpy166II GTNNAC 2 cut(s) 628, 992
Hpy188I TCNGA 5 cut(s) 121, 276, 363, 873, 1435
Hpy188III TCNNGA 4 cut(s) 155, 290, 617, 1572
Hpy8I GTNNAC 2 cut(s) 628, 992
Hpy99I CGWCG 5 cut(s) 50, 53, 125, 251, 397
HpyAV CCTTC 4 cut(s) 406, 659, 1039, 1588
HpyCH4III ACNGT 3 cut(s) 181, 625, 877
HpyCH4IV ACGT 2 cut(s) 45, 777
HpyF10VI GCNNNNNNNGC 8 cut(s) 35, 193, 347, 356, 817, 1130, 1451, 1460
HpyF3I CTNAG 2 cut(s) 254, 1113
HpySE526I ACGT 2 cut(s) 45, 777
HspAI GCGC 3 cut(s) 38, 818, 1064
Kzo9I GATC 6 cut(s) 30, 382, 613, 709, 862, 886
LmnI GCTCC 7 cut(s) 94, 122, 207, 277, 475, 763, 1472
Lsp1109I GCAGC 6 cut(s) 208, 350, 415, 1048, 1574, 1590
LweI GCATC 3 cut(s) 359, 626, 1188
MaeI CTAG 3 cut(s) 335, 342, 995
MaeII ACGT 2 cut(s) 45, 777
MaeIII GTNAC 5 cut(s) 1051, 1302, 1344, 1368, 1527
MalI GATC 6 cut(s) 32, 384, 615, 711, 864, 888
MboI GATC 6 cut(s) 30, 382, 613, 709, 862, 886
MflI RGATCY 1 cut(s) 613
MhlI GDGCHC 2 cut(s) 472, 723
MlsI TGGCCA 1 cut(s) 1621
MluNI TGGCCA 1 cut(s) 1621
MmeI TCCRAC 3 cut(s) 144, 741, 1167
Mox20I TGGCCA 1 cut(s) 1621
Mph1103I ATGCAT 3 cut(s) 352, 641, 1542
MscI TGGCCA 1 cut(s) 1621
MseI TTAA 7 cut(s) 603, 942, 1107, 1410, 1485, 1490, 1554
Msp20I TGGCCA 1 cut(s) 1621
MspCI CTTAAG 1 cut(s) 1409
MspI CCGG 3 cut(s) 88, 169, 426
MspR9I CCNGG 3 cut(s) 88, 822, 1442
MvaI CCWGG 2 cut(s) 822, 1442
MwoI GCNNNNNNNGC 8 cut(s) 35, 193, 347, 356, 817, 1130, 1451, 1460
NciI CCSGG 1 cut(s) 88
NcoI CCATGG 1 cut(s) 302
NdeII GATC 6 cut(s) 30, 382, 613, 709, 862, 886
NmuCI GTSAC 4 cut(s) 1051, 1344, 1368, 1527
NsiI ATGCAT 3 cut(s) 352, 641, 1542
NspI RCATGY 2 cut(s) 1321, 1610
PaeI GCATGC 1 cut(s) 1610
PciI ACATGT 1 cut(s) 1317
PfeI GAWTC 4 cut(s) 19, 190, 737, 946
PflMI CCANNNNNTGG 1 cut(s) 1233
PinAI ACCGGT 1 cut(s) 425
PkrI GCNGC 7 cut(s) 198, 228, 340, 405, 1038, 1564, 1605
PscI ACATGT 1 cut(s) 1317
Psp6I CCWGG 2 cut(s) 820, 1440
PspFI CCCAGC 1 cut(s) 716
PspGI CCWGG 2 cut(s) 820, 1440
PspPI GGNCC 1 cut(s) 1476
PstNI CAGNNNCTG 1 cut(s) 114
PsuI RGATCY 1 cut(s) 613
RsaI GTAC 3 cut(s) 280, 665, 1218
RsaNI GTAC 3 cut(s) 279, 664, 1217
SaqAI TTAA 7 cut(s) 603, 942, 1107, 1410, 1485, 1490, 1554
SatI GCNGC 7 cut(s) 197, 227, 339, 404, 1037, 1563, 1604
Sau3AI GATC 6 cut(s) 30, 382, 613, 709, 862, 886
Sau96I GGNCC 1 cut(s) 1476
ScaI AGTACT 1 cut(s) 280
ScrFI CCNGG 3 cut(s) 88, 822, 1442
SduI GDGCHC 2 cut(s) 472, 723
SfaNI GCATC 3 cut(s) 359, 626, 1188
SfcI CTRYAG 1 cut(s) 477
SinI GGWCC 1 cut(s) 1476
SmlI CTYRAG 1 cut(s) 1409
SmoI CTYRAG 1 cut(s) 1409
SphI GCATGC 1 cut(s) 1610
SsiI CCGC 2 cut(s) 226, 386
SspMI CTAG 3 cut(s) 335, 342, 995
StyD4I CCNGG 3 cut(s) 86, 820, 1440
StyI CCWWGG 1 cut(s) 302
TaaI ACNGT 3 cut(s) 181, 625, 877
TaiI ACGT 2 cut(s) 48, 780
TaqI TCGA 2 cut(s) 246, 1573
TaqII GACCGA 1 cut(s) 1493
TatI WGTACW 2 cut(s) 278, 1216
TauI GCSGC 1 cut(s) 229
TfiI GAWTC 4 cut(s) 19, 190, 737, 946
Tru1I TTAA 7 cut(s) 603, 942, 1107, 1410, 1485, 1490, 1554
Tru9I TTAA 7 cut(s) 603, 942, 1107, 1410, 1485, 1490, 1554
TscAI CASTG 2 cut(s) 29, 1056
TseFI GTSAC 4 cut(s) 1051, 1344, 1368, 1527
TseI GCWGC 6 cut(s) 196, 338, 403, 1036, 1562, 1603
Tsp45I GTSAC 4 cut(s) 1051, 1344, 1368, 1527
TspDTI ATGAA 5 cut(s) 323, 972, 1260, 1389, 1407
TspGWI ACGGA 3 cut(s) 107, 139, 648
TspRI CASTG 2 cut(s) 29, 1056
Van91I CCANNNNNTGG 1 cut(s) 1233
Vha464I CTTAAG 1 cut(s) 1409
VpaK11BI GGWCC 1 cut(s) 1476
XapI RAATTY 1 cut(s) 1144
XceI RCATGY 2 cut(s) 1321, 1610
XspI CTAG 3 cut(s) 335, 342, 995
ZrmI AGTACT 1 cut(s) 280
Zsp2I ATGCAT 3 cut(s) 352, 641, 1542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.