pycom16g12610

30S ribosomal protein S1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0013214
Physical Location & Seq
Forward (+)
8875859 .. 8877442
1584 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 678 bp
ATGGCGTCTCTGGCTCAGCAATTCACAGGCTTAAGATGCTCACCGCTCTCCACCTCGCGGCTCTCGAAGCCCTCAATCCTTCCCAAGCAAAACAAAATGCCGGGTTTGCTCCCCATCGTCTCCGCCGCGGTCATCTCGAATGCGCAGACCAAAGAGCGCCTCAAGCTGAAGGAAATCTTCGAAGACGCCCACGAACGGTGTCGTACAGCCCCCATGGAAGGCGTCTCTTTCACTCTCGACTCCTTTTACAACGCTCTCGAGAAGTACGACTTCAATTCTGAGATTGGAACTAAGGTGAGGGGGACAGTTTTCAATATAGATAACAATGGAGCATTAGTTGACATTACTGCAAAGTCTTCAGCATACTTGCCTTTGCAAGAGGCATGCATTCACAAAATAAAGAGTGTAGAAGAAGTGGGCATAGTTCCCGGAGTGAGAGAGGAATTTGTGATTATAGGCGAAAATGAAGCTGACGACAGCTTGATCTTGAGCTTGAAGTCCATCCAGTATGACCTTGCGTGGGAAAGATGTAGACAGCTCCAAGCTGAGGATGTTGTTGTCAAGGGTAAGGTTGTTGGTGCGAACAAAGGTGGAGTGGTGGCTGTGGTGGAAGGCCTTAGAGGTTTTGTTCCTTTCTCACAGATATCAACAAAATCAACCGCAGAAGATCGTTCTTGA

Protein Analysis

226

Amino Acids

24.58

Weight (kDa)

6.2

Isoelectric Point (pI)

45.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013214)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G30510
fragaria_vesca FvH4_4g20780 FvH4_4g20780
malus_domestica MD13G1147900.v1.1 MD16G1147700.v1.1
prunus_persica Prupe.1G111400_v2.0.a1
pyrus_communis pycom16g12610 pycom16g12620
rosa_chinensis RchiOBHm_Chr4g0426221
rosa_laevigata RLG00000007309
rosa_multiflora Rmu_sc0000730.1_g000026
rosa_roxburghii Rroxscaffold_5G00368240
rosa_rugosa Rorug04G0209700
rosa_samantha Rh4AG266600 Rh4BG272600 Rh4CG285800 Rh4DG268900
rosa_wichuraiana Rw4G023050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 144
AccBSI CCGCTC 1 cut(s) 46
AccI GTMKAC 1 cut(s) 532
AccII CGCG 2 cut(s) 58, 128
AciI CCGC 6 cut(s) 44, 58, 123, 126, 128, 660
AcsI RAATTY 1 cut(s) 443
AcuI CTGAAG 2 cut(s) 188, 342
AcyI GRCGYC 3 cut(s) 5, 186, 222
AfaI GTAC 2 cut(s) 205, 266
AfiI CCNNNNNNNGG 5 cut(s) 57, 195, 218, 520, 547
AflII CTTAAG 1 cut(s) 31
AgsI TTSAA 3 cut(s) 274, 313, 496
AluBI AGCT 6 cut(s) 166, 470, 480, 492, 538, 545
AluI AGCT 6 cut(s) 166, 470, 480, 492, 538, 545
Alw26I GTCTC 3 cut(s) 12, 124, 229
Ama87I CYCGRG 1 cut(s) 257
AoxI GGCC 1 cut(s) 613
ApoI RAATTY 1 cut(s) 443
AspLEI GCGC 2 cut(s) 145, 159
AsuC2I CCSGG 2 cut(s) 102, 429
AsuHPI GGTGA 2 cut(s) 33, 307
AsuII TTCGAA 1 cut(s) 180
AvaI CYCGRG 1 cut(s) 257
BbsI GAAGAC 2 cut(s) 189, 348
BbvCI CCTCAGC 1 cut(s) 546
BccI CCATC 2 cut(s) 122, 509
BcnI CCSGG 2 cut(s) 102, 429
BcoDI GTCTC 3 cut(s) 12, 124, 229
BfoI RGCGCY 1 cut(s) 160
BfrI CTTAAG 1 cut(s) 31
BisI GCNGC 2 cut(s) 59, 126
BlpI GCTNAGC 1 cut(s) 15
BlsI GCNGC 2 cut(s) 60, 127
Bme1390I CCNGG 2 cut(s) 102, 429
BmeT110I CYCGRG 1 cut(s) 257
BmrFI CCNGG 2 cut(s) 102, 429
BmsI GCATC 1 cut(s) 26
BpiI GAAGAC 2 cut(s) 189, 348
Bpu10I CCTNAGC 1 cut(s) 546
Bpu1102I GCTNAGC 1 cut(s) 15
Bpu14I TTCGAA 1 cut(s) 180
BpuEI CTTGAG 2 cut(s) 146, 508
BpuMI CCSGG 2 cut(s) 102, 429
BsaHI GRCGYC 3 cut(s) 5, 186, 222
BsaJI CCNNGG 2 cut(s) 126, 213
BsaXI ACNNNNNCTCC 2 cut(s) 321, 351
Bsc4I CCNNNNNNNGG 5 cut(s) 57, 195, 218, 520, 547
Bse1I ACTGG 1 cut(s) 505
BseDI CCNNGG 2 cut(s) 126, 213
BseGI GGATG 2 cut(s) 501, 556
BseLI CCNNNNNNNGG 5 cut(s) 57, 195, 218, 520, 547
BseMII CTCAG 3 cut(s) 29, 270, 537
BseNI ACTGG 1 cut(s) 505
Bsh1236I CGCG 2 cut(s) 58, 128
BshFI GGCC 1 cut(s) 615
BsiHKCI CYCGRG 1 cut(s) 257
BsiSI CCGG 2 cut(s) 101, 429
BslFI GGGAC 1 cut(s) 316
BslI CCNNNNNNNGG 5 cut(s) 57, 195, 218, 520, 547
BsmAI GTCTC 3 cut(s) 12, 124, 229
BsmBI CGTCTC 3 cut(s) 12, 124, 229
BsmFI GGGAC 1 cut(s) 316
BsmI GAATGC 2 cut(s) 145, 387
BsnI GGCC 1 cut(s) 615
BsoBI CYCGRG 1 cut(s) 257
Bsp119I TTCGAA 1 cut(s) 180
Bsp143I GATC 2 cut(s) 483, 667
Bsp1720I GCTNAGC 1 cut(s) 15
Bsp19I CCATGG 1 cut(s) 213
BspACI CCGC 6 cut(s) 44, 58, 123, 126, 128, 660
BspANI GGCC 1 cut(s) 615
BspCNI CTCAG 3 cut(s) 28, 271, 538
BspFNI CGCG 2 cut(s) 58, 128
BspT104I TTCGAA 1 cut(s) 180
BspTI CTTAAG 1 cut(s) 31
BsrBI CCGCTC 1 cut(s) 46
BsrI ACTGG 1 cut(s) 505
BssECI CCNNGG 2 cut(s) 126, 213
BssMI GATC 2 cut(s) 483, 667
BssNI GRCGYC 3 cut(s) 5, 186, 222
BssT1I CCWWGG 1 cut(s) 213
Bst4CI ACNGT 2 cut(s) 198, 307
BstACI GRCGYC 3 cut(s) 5, 186, 222
BstAFI CTTAAG 1 cut(s) 31
BstBI TTCGAA 1 cut(s) 180
BstC8I GCNNGC 1 cut(s) 385
BstDEI CTNAG 5 cut(s) 15, 279, 291, 546, 617
BstDSI CCRYGG 2 cut(s) 126, 213
BstF5I GGATG 2 cut(s) 501, 556
BstFNI CGCG 2 cut(s) 58, 128
BstH2I RGCGCY 1 cut(s) 160
BstHHI GCGC 2 cut(s) 145, 159
BstKTI GATC 2 cut(s) 486, 670
BstMAI GTCTC 3 cut(s) 12, 124, 229
BstMBI GATC 2 cut(s) 483, 667
BstMWI GCNNNNNNNGC 5 cut(s) 11, 36, 67, 106, 163
BstNSI RCATGY 1 cut(s) 387
BstSCI CCNGG 2 cut(s) 100, 427
BstUI CGCG 2 cut(s) 58, 128
BstV2I GAAGAC 2 cut(s) 189, 348
BsuRI GGCC 1 cut(s) 615
BtgI CCRYGG 2 cut(s) 126, 213
BtsCI GGATG 2 cut(s) 501, 556
Cac8I GCNNGC 1 cut(s) 385
CfoI GCGC 2 cut(s) 145, 159
Cfr42I CCGCGG 1 cut(s) 129
CseI GACGC 2 cut(s) 194, 211
Csp6I GTAC 2 cut(s) 204, 265
CviAII CATG 2 cut(s) 214, 384
CviQI GTAC 2 cut(s) 204, 265
DdeI CTNAG 5 cut(s) 15, 279, 291, 546, 617
DpnI GATC 2 cut(s) 485, 669
DpnII GATC 2 cut(s) 483, 667
EciI GGCGGA 1 cut(s) 112
Eco130I CCWWGG 1 cut(s) 213
Eco147I AGGCCT 1 cut(s) 615
Eco32I GATATC 1 cut(s) 645
Eco57I CTGAAG 2 cut(s) 188, 342
Eco88I CYCGRG 1 cut(s) 257
EcoRV GATATC 1 cut(s) 645
EcoT14I CCWWGG 1 cut(s) 213
EcoT22I ATGCAT 1 cut(s) 389
ErhI CCWWGG 1 cut(s) 213
Esp3I CGTCTC 3 cut(s) 12, 124, 229
FaeI CATG 2 cut(s) 217, 387
FaiI YATR 7 cut(s) 215, 317, 364, 385, 422, 455, 510
FalI AAGNNNNNCTT 4 cut(s) 161, 193, 254, 286
FaqI GGGAC 1 cut(s) 316
FatI CATG 2 cut(s) 213, 383
FblI GTMKAC 1 cut(s) 532
Fnu4HI GCNGC 2 cut(s) 59, 126
FokI GGATG 2 cut(s) 488, 563
Fsp4HI GCNGC 2 cut(s) 59, 126
FspI TGCGCA 1 cut(s) 144
GlaI GCGC 2 cut(s) 144, 158
GluI GCNGC 2 cut(s) 59, 126
HaeII RGCGCY 1 cut(s) 160
HaeIII GGCC 1 cut(s) 615
HapII CCGG 2 cut(s) 101, 429
HgaI GACGC 2 cut(s) 194, 211
HhaI GCGC 2 cut(s) 145, 159
Hin1I GRCGYC 3 cut(s) 5, 186, 222
Hin1II CATG 2 cut(s) 217, 387
Hin6I GCGC 2 cut(s) 143, 157
HinP1I GCGC 2 cut(s) 143, 157
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HinfI GANTC 1 cut(s) 239
HpaII CCGG 2 cut(s) 101, 429
HphI GGTGA 2 cut(s) 33, 307
Hpy166II GTNNAC 2 cut(s) 340, 533
Hpy188I TCNGA 1 cut(s) 280
Hpy188III TCNNGA 7 cut(s) 64, 136, 236, 257, 259, 487, 675
Hpy8I GTNNAC 2 cut(s) 340, 533
HpyAV CCTTC 4 cut(s) 89, 163, 212, 605
HpyCH4III ACNGT 2 cut(s) 198, 307
HpyCH4V TGCA 3 cut(s) 350, 376, 387
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 36, 67, 106, 163
HpyF3I CTNAG 5 cut(s) 15, 279, 291, 546, 617
Hsp92I GRCGYC 3 cut(s) 5, 186, 222
Hsp92II CATG 2 cut(s) 217, 387
HspAI GCGC 2 cut(s) 143, 157
KspI CCGCGG 1 cut(s) 129
Kzo9I GATC 2 cut(s) 483, 667
LmnI GCTCC 3 cut(s) 114, 329, 543
LpnPI CCDG 4 cut(s) 12, 114, 442, 518
LweI GCATC 1 cut(s) 26
MalI GATC 2 cut(s) 485, 669
MbiI CCGCTC 1 cut(s) 46
MboI GATC 2 cut(s) 483, 667
MboII GAAGA 5 cut(s) 169, 194, 348, 422, 677
MluCI AATT 3 cut(s) 20, 274, 443
MlyI GAGTC 1 cut(s) 233
MnlI CCTC 8 cut(s) 64, 82, 170, 291, 373, 433, 541, 614
Mph1103I ATGCAT 1 cut(s) 389
MseI TTAA 1 cut(s) 32
MspA1I CMGCKG 1 cut(s) 128
MspCI CTTAAG 1 cut(s) 31
MspI CCGG 2 cut(s) 101, 429
MspR9I CCNGG 2 cut(s) 102, 429
Mva1269I GAATGC 2 cut(s) 145, 387
MvnI CGCG 2 cut(s) 58, 128
MwoI GCNNNNNNNGC 5 cut(s) 11, 36, 67, 106, 163
NciI CCSGG 2 cut(s) 102, 429
NcoI CCATGG 1 cut(s) 213
NdeII GATC 2 cut(s) 483, 667
NlaIII CATG 2 cut(s) 217, 387
NsbI TGCGCA 1 cut(s) 144
NsiI ATGCAT 1 cut(s) 389
NspI RCATGY 1 cut(s) 387
NspV TTCGAA 1 cut(s) 180
PaeI GCATGC 1 cut(s) 387
PaeR7I CTCGAG 1 cut(s) 257
PceI AGGCCT 1 cut(s) 615
PcsI WCGNNNNNNNCGW 2 cut(s) 62, 264
PctI GAATGC 2 cut(s) 145, 387
PfoI TCCNGGA 1 cut(s) 427
PkrI GCNGC 2 cut(s) 60, 127
PleI GAGTC 1 cut(s) 233
PpsI GAGTC 1 cut(s) 233
RsaI GTAC 2 cut(s) 205, 266
RsaNI GTAC 2 cut(s) 204, 265
SacII CCGCGG 1 cut(s) 129
SaqAI TTAA 1 cut(s) 32
SatI GCNGC 2 cut(s) 59, 126
Sau3AI GATC 2 cut(s) 483, 667
SchI GAGTC 1 cut(s) 233
ScrFI CCNGG 2 cut(s) 102, 429
SfaNI GCATC 1 cut(s) 26
Sfr274I CTCGAG 1 cut(s) 257
Sfr303I CCGCGG 1 cut(s) 129
SfuI TTCGAA 1 cut(s) 180
SgrBI CCGCGG 1 cut(s) 129
SlaI CTCGAG 1 cut(s) 257
SmlI CTYRAG 4 cut(s) 31, 161, 257, 487
SmoI CTYRAG 4 cut(s) 31, 161, 257, 487
SphI GCATGC 1 cut(s) 387
Sse9I AATT 3 cut(s) 20, 274, 443
SseBI AGGCCT 1 cut(s) 615
SsiI CCGC 6 cut(s) 44, 58, 123, 126, 128, 660
StuI AGGCCT 1 cut(s) 615
StyD4I CCNGG 2 cut(s) 100, 427
StyI CCWWGG 1 cut(s) 213
TaaI ACNGT 2 cut(s) 198, 307
TaqI TCGA 5 cut(s) 65, 137, 180, 237, 258
TasI AATT 3 cut(s) 20, 274, 443
TauI GCSGC 2 cut(s) 61, 128
Tru1I TTAA 1 cut(s) 32
Tru9I TTAA 1 cut(s) 32
TspDTI ATGAA 1 cut(s) 480
Vha464I CTTAAG 1 cut(s) 31
XapI RAATTY 1 cut(s) 443
XceI RCATGY 1 cut(s) 387
XhoI CTCGAG 1 cut(s) 257
XmiI GTMKAC 1 cut(s) 532
Zsp2I ATGCAT 1 cut(s) 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.