pycom16g12620

30S ribosomal protein S1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0013214
Physical Location & Seq
Forward (+)
8877516 .. 8878425
910 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 489 bp
ATGGCAGACAACCAAGCACAGCTTGGAATTGGATCAGTTGTCATTGGAACTGTCCAGAGCCTGAAGCCATATGGTGCCTTTATCGACATTGGTGGAATCAATGGTCTTCTTCATGTTAGCCAGATCAGTCATGACCGAGTCTCAGATATTGCAACAGTTCTCCAACCTGGTGACACTCTCAAGGTCATGATATTGAGCCATGACCGTGAGAGAGGCCGTGTAAGTCTATCTACCAAGAAGTTAGAACCTACTCCTGGTGACATGATTCGCAACCCAACGCTTGTTTTCGAGAAGGCGGAGGAAATGGCACAGACATTCAGGCAGAGAATTGCTCAAGCAGAAGCTATGGCTCGTGCGGACATGCTCCGTTTCCAACCAGAGAGCGGATTAACCCTCAGCTCCGACGGAATCTTGGGTCCTCTTACTTCAGACTTGCCAGTAGAGGGTTTAGATTTGAGCGATGTGCCTCCGGCTGAAGTGACTGACTGA

Protein Analysis

163

Amino Acids

17.41

Weight (kDa)

4.79

Isoelectric Point (pI)

28.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1 PF00575 10 - 79 2.6e-18 S1 RNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013214)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G30510
fragaria_vesca FvH4_4g20780 FvH4_4g20780
malus_domestica MD13G1147900.v1.1 MD16G1147700.v1.1
prunus_persica Prupe.1G111400_v2.0.a1
pyrus_communis pycom16g12610 pycom16g12620
rosa_chinensis RchiOBHm_Chr4g0426221
rosa_laevigata RLG00000007309
rosa_multiflora Rmu_sc0000730.1_g000026
rosa_roxburghii Rroxscaffold_5G00368240
rosa_rugosa Rorug04G0209700
rosa_samantha Rh4AG266600 Rh4BG272600 Rh4CG285800 Rh4DG268900
rosa_wichuraiana Rw4G023050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 74
AccBSI CCGCTC 1 cut(s) 384
AciI CCGC 3 cut(s) 296, 356, 384
AclWI GGATC 1 cut(s) 40
AcuI CTGAAG 2 cut(s) 83, 411
AfiI CCNNNNNNNGG 3 cut(s) 254, 383, 443
AjnI CCWGG 2 cut(s) 166, 253
AluBI AGCT 3 cut(s) 22, 344, 399
AluI AGCT 3 cut(s) 22, 344, 399
Alw26I GTCTC 1 cut(s) 145
AlwI GGATC 1 cut(s) 40
AlwNI CAGNNNCTG 1 cut(s) 61
AoxI GGCC 1 cut(s) 214
AspS9I GGNCC 1 cut(s) 416
AsuHPI GGTGA 2 cut(s) 182, 269
AvaII GGWCC 1 cut(s) 416
BanI GGYRCC 1 cut(s) 74
BauI CACGAG 1 cut(s) 351
BbsI GAAGAC 1 cut(s) 98
BbvCI CCTCAGC 1 cut(s) 395
BceAI ACGGC 1 cut(s) 201
BciT130I CCWGG 2 cut(s) 168, 255
BcoDI GTCTC 1 cut(s) 145
Bme1390I CCNGG 2 cut(s) 168, 255
Bme18I GGWCC 1 cut(s) 416
BmgT120I GGNCC 1 cut(s) 416
BmiI GGNNCC 2 cut(s) 76, 417
BmrFI CCNGG 2 cut(s) 168, 255
BpiI GAAGAC 1 cut(s) 98
BplI GAGNNNNNCTC 2 cut(s) 316, 348
Bpu10I CCTNAGC 1 cut(s) 395
BpuEI CTTGAG 2 cut(s) 164, 318
Bsc4I CCNNNNNNNGG 3 cut(s) 254, 383, 443
Bse1I ACTGG 1 cut(s) 437
BseBI CCWGG 2 cut(s) 168, 255
BseLI CCNNNNNNNGG 3 cut(s) 254, 383, 443
BseMII CTCAG 2 cut(s) 156, 409
BseNI ACTGG 1 cut(s) 437
BshFI GGCC 1 cut(s) 216
BshNI GGYRCC 1 cut(s) 74
BsiSI CCGG 1 cut(s) 470
BslI CCNNNNNNNGG 3 cut(s) 254, 383, 443
BsmAI GTCTC 1 cut(s) 145
BsnI GGCC 1 cut(s) 216
Bsp143I GATC 2 cut(s) 32, 123
BspACI CCGC 3 cut(s) 296, 356, 384
BspANI GGCC 1 cut(s) 216
BspCNI CTCAG 2 cut(s) 155, 408
BspHI TCATGA 2 cut(s) 130, 186
BspLI GGNNCC 2 cut(s) 76, 417
BspPI GGATC 1 cut(s) 40
BspT107I GGYRCC 1 cut(s) 74
BsrBI CCGCTC 1 cut(s) 384
BsrI ACTGG 1 cut(s) 437
BssMI GATC 2 cut(s) 32, 123
BssSI CACGAG 1 cut(s) 351
Bst2BI CACGAG 1 cut(s) 351
Bst2UI CCWGG 2 cut(s) 168, 255
Bst4CI ACNGT 3 cut(s) 52, 157, 206
BstDEI CTNAG 2 cut(s) 142, 395
BstKTI GATC 2 cut(s) 35, 126
BstMAI GTCTC 1 cut(s) 145
BstMBI GATC 2 cut(s) 32, 123
BstNI CCWGG 2 cut(s) 168, 255
BstNSI RCATGY 1 cut(s) 364
BstSCI CCNGG 2 cut(s) 166, 253
BstV2I GAAGAC 1 cut(s) 98
BsuRI GGCC 1 cut(s) 216
BtgZI GCGATG 1 cut(s) 474
CaiI CAGNNNCTG 1 cut(s) 61
CciI TCATGA 2 cut(s) 130, 186
Cfr13I GGNCC 1 cut(s) 416
CsiI ACCWGGT 1 cut(s) 166
CviAII CATG 6 cut(s) 113, 131, 187, 200, 262, 361
DdeI CTNAG 2 cut(s) 142, 395
DpnI GATC 2 cut(s) 34, 125
DpnII GATC 2 cut(s) 32, 123
EciI GGCGGA 1 cut(s) 311
Eco47I GGWCC 1 cut(s) 416
Eco57I CTGAAG 2 cut(s) 83, 411
EcoO109I RGGNCCY 1 cut(s) 416
EcoRII CCWGG 2 cut(s) 166, 253
FaeI CATG 6 cut(s) 116, 134, 190, 203, 265, 364
FaiI YATR 9 cut(s) 70, 72, 114, 132, 188, 201, 263, 347, 362
FalI AAGNNNNNCTT 1 cut(s) 38
FatI CATG 6 cut(s) 112, 130, 186, 199, 261, 360
FauNDI CATATG 1 cut(s) 70
HaeIII GGCC 1 cut(s) 216
HapII CCGG 1 cut(s) 470
Hin1II CATG 6 cut(s) 116, 134, 190, 203, 265, 364
HinfI GANTC 4 cut(s) 96, 138, 265, 408
HpaII CCGG 1 cut(s) 470
HphI GGTGA 2 cut(s) 182, 269
Hpy188I TCNGA 3 cut(s) 145, 403, 430
Hpy188III TCNNGA 4 cut(s) 55, 131, 187, 289
Hpy99I CGWCG 1 cut(s) 407
HpyAV CCTTC 1 cut(s) 286
HpyCH4III ACNGT 3 cut(s) 52, 157, 206
HpyCH4V TGCA 1 cut(s) 152
HpyF3I CTNAG 2 cut(s) 142, 395
Hsp92II CATG 6 cut(s) 116, 134, 190, 203, 265, 364
Kzo9I GATC 2 cut(s) 32, 123
LmnI GCTCC 2 cut(s) 369, 404
MabI ACCWGGT 1 cut(s) 166
MaeIII GTNAC 3 cut(s) 170, 257, 478
MalI GATC 2 cut(s) 34, 125
MbiI CCGCTC 1 cut(s) 384
MboI GATC 2 cut(s) 32, 123
MboII GAAGA 2 cut(s) 98, 101
MluCI AATT 2 cut(s) 27, 327
MlyI GAGTC 1 cut(s) 147
MmeI TCCRAC 3 cut(s) 187, 397, 426
MnlI CCTC 6 cut(s) 206, 292, 404, 429, 436, 477
MseI TTAA 1 cut(s) 389
MslI CAYNNNNRTG 1 cut(s) 204
MspI CCGG 1 cut(s) 470
MspR9I CCNGG 2 cut(s) 168, 255
MvaI CCWGG 2 cut(s) 168, 255
NdeI CATATG 1 cut(s) 70
NdeII GATC 2 cut(s) 32, 123
NlaIII CATG 6 cut(s) 116, 134, 190, 203, 265, 364
NlaIV GGNNCC 2 cut(s) 76, 417
NmuCI GTSAC 3 cut(s) 170, 257, 478
NspI RCATGY 1 cut(s) 364
PagI TCATGA 2 cut(s) 130, 186
PfeI GAWTC 3 cut(s) 96, 265, 408
PflFI GACNNNGTC 1 cut(s) 137
PleI GAGTC 1 cut(s) 146
PpsI GAGTC 1 cut(s) 146
PpuMI RGGWCCY 1 cut(s) 416
Psp5II RGGWCCY 1 cut(s) 416
Psp6I CCWGG 2 cut(s) 166, 253
PspGI CCWGG 2 cut(s) 166, 253
PspN4I GGNNCC 2 cut(s) 76, 417
PspPI GGNCC 1 cut(s) 416
PspPPI RGGWCCY 1 cut(s) 416
PstNI CAGNNNCTG 1 cut(s) 61
PsyI GACNNNGTC 1 cut(s) 137
RseI CAYNNNNRTG 1 cut(s) 204
SaqAI TTAA 1 cut(s) 389
Sau3AI GATC 2 cut(s) 32, 123
Sau96I GGNCC 1 cut(s) 416
SchI GAGTC 1 cut(s) 147
ScrFI CCNGG 2 cut(s) 168, 255
SetI ASST 6 cut(s) 24, 169, 186, 250, 346, 401
SexAI ACCWGGT 1 cut(s) 166
SinI GGWCC 1 cut(s) 416
SmiMI CAYNNNNRTG 1 cut(s) 204
SmlI CTYRAG 2 cut(s) 179, 333
SmoI CTYRAG 2 cut(s) 179, 333
Sse9I AATT 2 cut(s) 27, 327
SsiI CCGC 3 cut(s) 296, 356, 384
StyD4I CCNGG 2 cut(s) 166, 253
TaaI ACNGT 3 cut(s) 52, 157, 206
TaqI TCGA 2 cut(s) 84, 288
TaqII GACCGA 1 cut(s) 150
TasI AATT 2 cut(s) 27, 327
TfiI GAWTC 3 cut(s) 96, 265, 408
Tru1I TTAA 1 cut(s) 389
Tru9I TTAA 1 cut(s) 389
TseFI GTSAC 3 cut(s) 170, 257, 478
Tsp45I GTSAC 3 cut(s) 170, 257, 478
TspDTI ATGAA 1 cut(s) 101
TspGWI ACGGA 2 cut(s) 356, 420
Tth111I GACNNNGTC 1 cut(s) 137
VpaK11BI GGWCC 1 cut(s) 416
XceI RCATGY 1 cut(s) 364
XcmI CCANNNNNNNNNTGG 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.